Publications

[significance of determination of shigella antibiotic resistance in bacteriological diagnosis of dysentery].	the results of the shigella antibiotic susceptibility assay within 1995-2002 are presented. 1472 cultures from 1158 patients with intestinal infections and bacteria carriers were isolated. the isolates were tested for their susceptibility to tetracycline, chloramphenicol, gentamicin, kanamycin, ampicillin and ofloxacin. it was shown that s. flexneri and s. sonnei were resistant to tetracycline. the s. flexneri isolates were highly resistant to chloramphenicol (73.3 to 96.0%) while resistance to ...	2005	16308937
fur regulates acid resistance in shigella flexneri via ryhb and ydep.	shigella flexneri requires iron for survival, and the genes for iron uptake and homeostasis are regulated by the fur protein. microarrays were used to identify genes regulated by fur and to study the physiological effects of iron availability in s. flexneri. these assays showed that the expression of genes involved in iron acquisition and acid response was induced by low-iron availability and by inactivation of fur. a fur null mutant was acid sensitive in media at ph 2.5, and acid sensitivity wa ...	2005	16313621
[construction and characterization of a live attenuated shigella flexneri 2a vaccine strain, sf301 delta virg and dsba33g].	construction and characterization of a live attenuated shigella flexneria 2a sf301 vaccine strain to prevent the endemic of shigellosis. using chinese majority epidemic shigella flexneri 2a serotype sf301 as the target, p delta virg, a deletion derivation of the virg gene in the sacb suicide vector pcvd442 and pdsba33g, an mutant of a disulfide bond catalyst dsba, replaced its 33 amiano acid cystine by glycerin in pcvd442, were used to generate a attenuated mutant strain sf301: delta virg: dsba3 ...	2005	16342769
quinine sulfate inhibits invasion of salmonella typhimurium and shigella flexneri: a preliminary study.	the incidence of typhoid fever and shigellosis parallels that of malaria, so many individuals who are on antimalarial drugs can be found in areas where these diseases are widespread. we investigated the effect of quinine sulfate on the growth and invasion of salmonella typhimurium and shigella flexneri m90t to determine whether people on antimalarials can have secondary gain from some protection against typhoid fever and shigellosis.	2005	16343387
[effects of different compositions of gegenqinlian decoction on experimental shigellosis in rabbits].	to investigate the relations between different compositions of gegenqinlian decoction and their effects on experimental shigellosis in rabbits to explore the composition protocol of gegenqinlian decoction.	2005	16174580
an unusual case of diarrhea.	the clue to this patient's correct diagnosis was his recent return from india. he usually visits there on an annual basis and the occurrence of posttravel infection has been noted in his medical records. this case study shows the importance of assessing patients for fever, abdominal cramps and diarrhea after recent travel outside of the united states. shigella gastroenteritis should be in the differential diagnosis for patients with the above clinical presentation. the keys for diagnosis and pro ...	2005	16180784
a bacterial group ii intron-encoded reverse transcriptase localizes to cellular poles.	the lactococcus lactis ll.ltrb group ii intron encodes a reverse transcriptase (ltra protein) that binds the intron rna to promote rna splicing and intron mobility. here, we used ltra-gfp fusions and immunofluorescence microscopy to show that ltra localizes to cellular poles in escherichia coli and lactococcus lactis. this polar localization occurs with or without coexpression of ll.ltrb intron rna, is observed over a wide range of cellular growth rates and expression levels, and is independent ...	2005	16186487
[dysentery in poland in 2003].	the notified number of dysentery cases is the lowest in this century and even every year decreasing. only 75 cases were notified in the year 2003 (incidence rate 0.20/100 000 population) while 220 cases were registered in 2002 (incidence 0.58/100 000 population). no one death case was notified. the last three death cases were notified in 1999. only one outbreak of dysentery (due to s. flexneri 2a) was registered involving 23 patients/111 residents of social home for mentally disabled men. source ...	2005	16190529
abl kinases regulate actin comet tail elongation via an n-wasp-dependent pathway.	microbial pathogens have evolved diverse strategies to modulate the host cell cytoskeleton to achieve a productive infection and have proven instrumental for unraveling the molecular machinery that regulates actin polymerization. here we uncover a mechanism for shigella flexneri-induced actin comet tail elongation that links abl family kinases to n-wasp-dependent actin polymerization. we show that the abl kinases are required for shigella actin comet tail formation, maximal intracellular motilit ...	2005	16199863
the needle component of the type iii secreton of shigella regulates the activity of the secretion apparatus.	gram-negative bacteria commonly interact with eukaryotic host cells by using type iii secretion systems (ttsss or secretons). ttsss serve to transfer bacterial proteins into host cells. two translocators, ipab and ipac, are first inserted with the aid of ipad by shigella into the host cell membrane. then at least two supplementary effectors of cell invasion, ipaa and ipgd, are transferred into the host cytoplasm. how ttsss are induced to secrete is unknown, but their activation appears to requir ...	2005	16227202
the epidemiology of travel-associated shigellosis--regional risks, seasonality and serogroups.	to give a detailed risk estimate of contracting travel-associated shigellosis in various regions of the world.	2005	16230220
the burden of diarrhoea, shigellosis, and cholera in north jakarta, indonesia: findings from 24 months surveillance.	in preparation of vaccines trials to estimate protection against shigellosis and cholera we conducted a two-year community-based surveillance study in an impoverished area of north jakarta which provided updated information on the disease burden in the area.	2005	16242013
functional effects of japanese style fermented soy sauce (shoyu) and its components.	the functional effects of japanese style fermented soy sauce (shoyu) have been studied. soy sauce promotes digestion, because the consumption of a cup of clear soup containing soy sauce enhances gastric juice secretion in humans. soy sauce possesses antimicrobial activity against bacteria such as staphylococcus aureus, shigella flexneri, vibrio cholera, salmonella enteritidis, nonpathogenic escherichia coli and pathogenic e. coli o157:h7. soy sauce also contains an antihypertensive component. an ...	2005	16243270
helical packing of needles from functionally altered shigella type iii secretion systems.	gram-negative bacteria commonly interact with eukaryotic host cells using type iii secretion systems (ttsss or secretons), which comprise cytoplasmic, transmembrane and extracellular domains. the extracellular domain is a hollow needle-like structure protruding 60 nm beyond the bacterial surface. the ttss is activated to transfer bacterial proteins directly into a host cell only upon physical contact with the target cell. we showed previously that the monomer of the shigella flexneri needle, mxi ...	2005	16243352
risk factors for mortality due to shigellosis: a case-control study among severely-malnourished children in bangladesh.	to determine the risk factors for death of severely-malnourished bangladeshi children with shigellosis, a case-control study was conducted at the clinical research and service centre of icddr,b: centre for health and population research in dhaka, bangladesh. one hundred severely-malnourished children (weight-for-age <60% of median of the national center for health statistics), with a positive stool culture for shigella dysenteriae type 1 or s. flexneri, who died during hospitalization, were comp ...	2005	16262023
the mreb and min cytoskeletal-like systems play independent roles in prokaryotic polar differentiation.	establishment of an axis of cell polarity and differentiation of the cell poles are fundamental aspects of cellular development in many organisms. we compared the effects of two bacterial cytoskeletal-like systems, the mreb and mincde systems, on these processes in escherichia coli. we report that the min proteins are capable of establishing an axis of oscillation that is the initial step in establishment of polarity in spherical cells, in a process that is independent of the mreb cytoskeleton. ...	2005	16262780
construction of prophylactic human papillomavirus type 16 l1 capsid protein vaccine delivered by live attenuated shigella flexneri strain sh42.	to express human papillomavirus (hpv) l1 capsid protein in the recombinant strain of shigella and study the potential of a live attenuated shigella-based hpv prophylactic vaccine in preventing hpv infection, the icsa/virg fragment of shigella-based prokaryotic expression plasmid phs3199 was constructed. hpv type 16 l1 (hpv16l1) gene was inserted into plasmid phs3199 to form the phs3199-hpv16l1 construct, and phs3199-hpv16l1 was electroporated into a live attenuated shigella strain sh42. western ...	2005	16270153
shigella flexneri perinephric abscess and bacteremia.		2005	16270768
genome dynamics and diversity of shigella species, the etiologic agents of bacillary dysentery.	the shigella bacteria cause bacillary dysentery, which remains a significant threat to public health. the genus status and species classification appear no longer valid, as compelling evidence indicates that shigella, as well as enteroinvasive escherichia coli, are derived from multiple origins of e.coli and form a single pathovar. nevertheless, shigella dysenteriae serotype 1 causes deadly epidemics but shigella boydii is restricted to the indian subcontinent, while shigella flexneri and shigel ...	2005	16275786
immunoproteomics of membrane proteins of shigella flexneri 2a 2457t.	to screen the immunogenic membrane proteins of shigella flexneri 2a 2457t.	2005	16425402
preparation, crystallization and preliminary x-ray crystallographic studies of diadenosine tetraphosphate hydrolase from shigella flexneri 2a.	diadenosine tetraphosphate (ap4a) hydrolase (ec 3.6.1.41) hydrolyzes ap4a symmetrically in prokaryotes. it plays a potential role in organisms by regulating the concentration of ap4a in vivo. to date, no three-dimensional structures of proteins with significant sequence homology to this protein have been determined. the 31.3 kda ap4a hydrolase from shigella flexneri 2a has been cloned, expressed and purified using an escherichia coli expression system. crystals of ap4a hydrolase have been obtain ...	2005	16511239
[the colicynogenic activity and anthibiotic sensitivity of collected and newly isolated strains of shigella flexneri and shigella sonnei].	among the collection strains of s. flexneri 10.5; 5.5; 2.6; 2.6 and 7.9% of strains were resistant to ampicillin, tetracycline, ceftryaxon, cefasoline, chloramphenycol, respectively. the maximum number of r-determinants of one strain was 4. the strains resistant to cefalosporynes were not revealed; among newly isolated strains of this agent, these strains resistant to ampicilline, tetracycline and chloramphenycol made 20%. among the collection and newly isolated strains of s. flexneri, isolated ...	2005	16396114
cephalosporin resistant shigella flexneri from a clinical isolate--a rare finding.	shigellosis is an important public health problem, especially in developing countries. antibiotic treatment of dysentery aimed at resolving diarrhea or reducing its duration, and to prevent transmission to other close contacts. isolates with resistance to first line drugs have been reported throughout world, third generation cephalosporins and quinolones are mainstay of treatment. shigella flexneri resistant to third generation cephalosporins in a clinical isolate is a rare finding, and this has ...	2005	16438280
viability of escherichia coli topa mutants lacking dna topoisomerase i.	the viability of the topa mutants lacking dna topoisomerase i was thought to depend on the presence of compensatory mutations in escherichia coli but not salmonella typhimurium or shigella flexneri. this apparent discrepancy in topa requirements in different bacteria prompted us to reexamine the topa requirements in e. coli. we find that e. coli strains bearing topa mutations, introduced into the strains by dna-mediated gene replacement, are viable at 37 or 42 degrees c without any compensatory ...	2005	15522872
the generalized transducing salmonella bacteriophage es18: complete genome sequence and dna packaging strategy.	the generalized transducing double-stranded dna bacteriophage es18 has an icosahedral head and a long noncontractile tail, and it infects both rough and smooth salmonella enterica strains. we report here the complete 46,900-bp genome nucleotide sequence and provide an analysis of the sequence. its 79 genes and their organization clearly show that es18 is a member of the lambda-like (lambdoid) phage group; however, it contains a novel set of genes that program assembly of the virion head. most of ...	2005	15659686
a novel intranasal protollin-based measles vaccine induces mucosal and systemic neutralizing antibody responses and cell-mediated immunity in mice.	protollin-mv is a vaccine produced by mixing split measles virus (mv) antigen with the novel adjuvant protollin (neisseria meningitidis outer membrane proteins non-covalently complexed with shigella flexneri 2a lipopolysaccharide). intranasal immunization of mice with two or three doses of protollin-mv induces both serum igg and mucosal iga with strong neutralizing activity. there is a dose-dependent shift towards lower igg1:igg2a ratios and mv-specific ifngamma production in splenocytes. intran ...	2005	15661386
modulation of an outer membrane protease contributes to the virulence defect of shigella flexneri strains carrying a mutation in the virk locus.	the shigella actin assembly protein icsa is removed from the bacterial surface by the protease icsp. we show that decreased intracellular spreading of virk::tn10 mutants is due in part to significant increases in icsp and icsp-mediated cleavage of icsa and that icsp expression is a critical determinant of shigella virulence.	2005	15664968
sensitive and rapid detection of shigella and enteroinvasive escherichia coli by a loop-mediated isothermal amplification method.	here we report a loop-mediated isothermal amplification (lamp) method for detecting shigella and enteroinvasive escherichia coli. the target for this lamp method is the ipah gene which is carried by both of the pathogens. the lamp method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 cfu) per reaction.	2005	15668027
synthesis of two linear padre conjugates bearing a deca- or pentadecasaccharide b epitope as potential synthetic vaccines against shigella flexneri serotype 2a infection.	the blockwise synthesis of the 2-aminoethyl glycosides of a deca- and a pentadecasaccharide made of two and three repeating units, respectively, of the shigella flexneri serotype 2a specific polysaccharide is reported. the strategy relies on trifluoromethanesulfonic acid mediated glycosylation of a pentasaccharide building block acting as a glycosyl donor and a potential glycoside acceptor. both targets were made available in amounts large enough for their subsequent conversion into glycoconjuga ...	2005	15669066
physiological stress of intracellular shigella flexneri visualized with a metabolic sensor fused to a surface-reporter system.	when deleted of its n-terminal signal-reception domain, the broad host range sigma54-dependent transcriptional regulator xylr, along with its cognate promoter pu, becomes a sensor of the metabolic stress of the carrier bacteria. we have employed a surface reporter system to visualize the physiological status of intracellular shigella flexneri during infection of henle 407 cells in culture. to this end, the xylrdeltaa gene has been engineered adjacent to a bicistronic transcriptional fusion of pu ...	2005	15670852
cloning of a novel gene for quinolone resistance from a transferable plasmid in shigella flexneri 2b.	a novel gene for quinolone resistance was cloned from a transferable plasmid carried by a clinical isolate of shigella flexneri 2b that was resistant to fluoroquinolones. the plasmid conferred low-level resistance to quinolones on escherichia coli hb101. the protein encoded by the gene showed 59% amino acid identity with qnr.	2005	15673773
occurrence of integrons and resistance genes among sulphonamide-resistant shigella spp. from brazil.	to determine the occurrence of class 1 and 2 integrons and antimicrobial resistance genes among sulphonamide-resistant shigella strains isolated in brazil during 1999-2003.	2005	15681578
a novel caspase-1/toll-like receptor 4-independent pathway of cell death induced by cytosolic shigella in infected macrophages.	shigella-induced macrophage cell death is an important step in the induction of acute inflammatory responses that ultimately lead to bacillary dysentery. cell death was previously reported to be dependent upon the activation of caspase-1 via interaction with ipab secreted by intracellular shigella, but in this study, we show that shigella infection of macrophages can also induce cell death independent of caspase-1 or ipab activity. time-lapse imaging and electron microscopic analyses indicated t ...	2005	15695506
a novel shigella dysenteriae serovar isolated in canada.	the etiological agent most commonly associated with bacillary dysentery is shigella. as part of its mandate, the bacteriology and enteric disease program of health canada identifies and serotypes unusual isolates of shigella received from provincial laboratories of public health. in this report, six unusual isolates from three provinces were analyzed biochemically and serologically using slide and tube agglutinations and molecularly using standard pulsed-filed gel electrophoresis (pfge), pcr, an ...	2005	15695673
effects of zinc supplementation as adjunct therapy on the systemic immune responses in shigellosis.	zinc is lost during diarrheal diseases, and zinc deficiency induces intestinal morphology-altering inflammatory responses that zinc supplementation can correct.	2005	15699240
etiology of diarrhea in pediatric outpatient settings.	the frequency with which bacteria cause diarrhea evaluated in ambulatory settings is often unknown. we attempted to determine the microbiologic etiology of diarrhea in a private pediatric practice (site a) and a clinic serving largely immigrant children (site b) and to establish guidelines for bacterial culture.	2005	15702043
serum amyloid a protein binds to outer membrane protein a of gram-negative bacteria.	serum amyloid a (saa) is the major acute phase protein in man and most mammals. we observed saa binding to a surprisingly large number of gram-negative bacteria, including escherichia coli, salmonella typhimurium, shigella flexneri, klebsiella pneumoniae, vibrio cholerae, and pseudomonas aeruginosa. the binding was found to be high affinity and rapid. importantly, this binding was not inhibited by high density lipoprotein with which saa is normally complexed in serum. binding was also observed w ...	2005	15705572
detection of virulence genes in malaysian shigella species by multiplex pcr assay.	in malaysia, shigella spp. was reported to be the third commonest bacterial agent responsible for childhood diarrhoea. currently, isolation of the bacterium and confirmation of the disease by microbiological and biochemical methods remain as the "gold standard". this study aimed to detect the prevalence of four shigella virulence genes present concurrently, in randomly selected malaysian strains via a rapid multiplex pcr (mpcr) assay.	2005	15707504
topological analysis of gtra and gtrb proteins encoded by the serotype-converting cassette of shigella flexneri.	serotype conversion (o-antigen glucosylation) in shigella flexneri is mediated by temperate bacteriophages, which encode a three-gene cluster that contains gtra, gtrb, and gtr([type]). sequence analysis has revealed that gtra and gtrb are conserved and readily interchangeable between serotypes. the gtr([type]) is unique in each serotype and responsible for specifically mediating conversion by the addition of a glucosyl group to the o-antigen units. analysis of the gtra and gtrb amino acid sequen ...	2005	15708010
gastroenteritis in us marines during operation iraqi freedom.	approximately 83,000 us marines participated in the opening phase of operation iraqi freedom in spring 2003. a navy preventive medicine laboratory was set up in ad diwaniyah, iraq, to provide clinical diagnostic support for marine medical units during a period of repositioning in south-central iraq.	2005	15712073
transcriptional adaptation of shigella flexneri during infection of macrophages and epithelial cells: insights into the strategies of a cytosolic bacterial pathogen.	shigella flexneri, the etiologic agent of bacillary dysentery, invades epithelial cells as well as macrophages and dendritic cells and escapes into the cytosol soon after invasion. dissection of the global gene expression profile of the bacterium in its intracellular niche is essential to fully understand the biology of shigella infection. we have determined the complete gene expression profiles for s. flexneri infecting human epithelial hela cells and human macrophage-like u937 cells. approxima ...	2005	15618144
construction and characterization of bivalent shigella flexneri 2a vaccine strains sc608(pcfai) and sc608(pcfai/ltb) that express antigens from enterotoxigenic escherichia coli.	an invasive strain of shigella flexneri 2a (sc608) has been developed as a vector for the expression and delivery of heterologous antigens. sc608 is an aspartate semialdehyde dehydrogenase (asd) derivative of sc602 (icsa iuc), a well-characterized live attenuated vaccine strain which has undergone several clinical trials in human volunteers. when administered orally at a single 10(4) (cfu) dose, sc602 is both immunogenic and efficacious against shigellosis. using asd-based plasmid vectors, we de ...	2005	15618162
virulent shigella flexneri causes damage to mitochondria and triggers necrosis in infected human monocyte-derived macrophages.	shigella flexneri is a gram-negative bacterium that causes bacillary dysentery in humans that is characterized by an acute inflammatory response of the colon. the fate of phagocytes that are infected in vitro with virulent shigella has been the subject of some investigation and debate. in this study we found that virulent shigella caused a rapid increase in the cell membrane permeability of infected human monocyte-derived macrophages (hmdm) but not in the cell membrane permeability of monocytes, ...	2005	15618190
crystal structure of the copper homeostasis protein (cutcm) from shigella flexneri at 1.7 a resolution: the first structure of a new sequence family of tim barrels.		2005	15624211
bacteria spurned by self-absorbed cells.		2005	15635436
interleukin-1 inhibits pge2 binding to macrophage-like p388d1 cells by a cyclic amp-independent process.	the interaction between interleukin il-1 alpha and pge2 on p388d1 cells has been investigated. preincubation of murine macrophage-like cells, p388d1, with il-1 alpha (0-73 pm) reduced the binding of pge2 to these cells in a concentration-dependent manner. scatchard analysis showed that il-1 alpha decreased the pge2 binding by lowering both the high and low affinity receptor binding capacities (from 0.31 +/- 0.02 to 0.12 +/- 0.01 fmol/10(6) cells for the high affinity receptor binding sites and f ...	2005	1310627
diphtheria toxin entry: protein translocation in the reverse direction.		2005	3072713
intracellular recordings of rod responses during dark-adaptation.	1. dark-adaptation of rod photoreceptors has been studied in the isolated axolotl (ambystoma mexicanum) retina by intracellular recordings. rod responsiveness was greatly reduced immediately after a 30 sec partial bleach, but partially recovered with time in the dark. 2. in parallel spectrophotometric measurements using isolated retinas, regeneration of the rod pigment could not be detected after a 30 sec bleach. 3. during rod dark-adaptation, the response of a rod to a given stimulus increased ...	2005	1151778
transient response of a solid-liquid model biological fluidised bed to a step change in fluid superficial velocity.	the evolution of a solid-liquid model biological fluidised bed under a step change in fluid superficial velocity is described. during a transient step change, the fluidised bed divides into a top zone which remains at the initial porosity and a bottom zone which settles at the final porosity. the interface of discontinuity in porosity moves progressively upwards through the fluidised bed. the velocity at which the top of the fluidised bed expands or contracts and the upward velocity of the poros ...	2005	1366699
[spontaneous closure of the interventricular septal defects in children].	a total of 214 patients with a defect of the interventricular septum have been examined starting from the first days of their life and to the age of 7 years. in 50 of them the defect closed spontaneously (23.4 per cent). twenty of these patients had a complicated course of the defect (circulatory insufficiency, colds and pneumonia). the spontaneous closure of the interventricular septum defect occurred most frequently at the age of from 1 to 4 years (86 per cent).	2005	1142609
[emergency treatment of acute promyelocytic leukemia and its hemorrhagic complications. apropos of 30 cases].		2005	1065249
lymphocyte markers and circulating immune complexes in primary glomerulonephritis.		2005	549018
[outpatient care in industrial communities as a means of reducing periodontal diseases].		2005	545607
[aspects of culture-media research within the scope of industrial vaccine production. summary of twenty-year development work in the institute for vaccines dessau].		2005	4725026
stroke rehabilitation. part i. general considerations.		2005	1214691
[the candida prostato-urethritis].		2005	1196083
the chemical use, abuse, and dependence scale (cuad). rationale, reliability, and validity.	this article describes the rationale for the development of the chemical use, abuse, and dependence scale (cuad). the instrument is in a semistructured interview format; it derives both substance use severity scores and dsm-iii-r substance use disorder diagnoses and can be administered in a short period with minimal training. the reliability and validity of the cuad are reported and appear satisfactory. the cuad is recommended for use as a detection, diagnostic, and treatment selection index in ...	2005	1317464
myelin specific, autoaggressive t cell clones in the normal immune repertoire: their nature and their regulation.		2005	1285063
part ii: the photic environment of clear tropical seas during the day.		2005	1166605
projections from the retina to the lateral geniculate nucleus and mesencephalic tectum in a reptile (tupinambis nigropunctatus): a comparison of anterograde transport and anterograde degeneration.		2005	1111840
[massive lower gastrointestinal hemorrhage of diverticular origin. diagnostic and therapeutic problems].		2005	1082809
inflammatory response linked to oxazolone-indued cutaneous basophil hypersensitivity: effect of different immunomodulator and anti-inflammatory drugs.	the potency of cytostatic and anti-inflammatory drugs was tested on the oxazolone-induced cutaneous basophil hypersensitivity (cbh) in mice. the challenge reaction was performed early after sensitization in order to minimize b-cell expression; exudative and cellular infiltration was estimated 6 h, 24 h and 48 h after challenge. the potency of drugs was tested at three different periods of immunization: 2 days before or after sensitization or before challenge. cytostatics act mainly when cells ar ...	2005	546176
[rupture of the distal tendon of the biceps brachii].		2005	538842
the molecular mechanisms of cardiovascular and renal regulation by endothelin peptides.	in summary, we have seen that endothelins are potent cardiovascular and renal regulatory peptides. cardiovascular regulation by endothelin requires complex spatial and temporal regulation of endothelin gene expression and the coordinated interplay of numerous signaling pathways. although the precise physiologic role for endothelin peptides in the cardiovascular and renal systems remains uncertain, two general models can be proposed, as follows. (1) endothelin appears to act as an autocrine or pa ...	2005	1317401
study of lipid changes in freeze-dried fish during storage. i. the interaction of relative humidity and tissue lipids.	several studies on lipid stability of freeze-dried fish pointed out the relationship between relative humidity (rh) and autoxidation of highly unsaturated fatty acids. the present study shows the changes of tissue lipids in two different fish, freeze-dried and stored under various rh conditions. fillets of scomber scomber l and sardina pilchardus sardina were freeze-dried and stored for 1, 3, 6 and 9 months. periodically, the sample of both fish were analyzed to evaluate the autoxidation of lipi ...	2005	1307019
feeling good affects your work. a team approach to health and productivity.		2005	1291285
cancer metastasis--a multistep process.	dermatologists are confronted daily with proliferative growths which can reach considerable size, but mean no danger to the patient. seborrheic keratoses are one example. other growths, such as basal cell carcinoma, are locally destructive. normally, however, we do not hesitate to reassure the patient about the benignity of the tumor once excised. the malignancy of squamous cell carcinomas and melanoma, however, is undoubted. thus, excessive proliferation and even local invasiveness are not suff ...	2005	1280176
[cryosurgery in the treatment of skin and mouth mucosa neoplasms].		2005	1272974
[psychiatric side-effects of levodopa treatment].		2005	1265908
circadian properties of the rhythmic system in individual nucleated and enucleated cells of acetabularia mediterranea.		2005	1248525
[epidemic haemorrhagic conjunctivitis in the middle east].		2005	1230937
fibrinolytic activity in malignant disease.	resting fibrinolytic activity and fibrinolytic capacity were compared in 31 patients with malignant disease and in 24 control subjects without malignant disease. patients with malignant disease had a lower mean fibrinolytic activity: this was particularly marked in those with disseminated disease. in contrast, patients with malignant disease had a fibrinolytic capacity which did not differ from that of the control subjects.	2005	1236626
[drug pulverizer with a spatula].		2005	1231142
is poliomyelitis a genetically-determined disease? ii. a critical examination of the epidemiological data.		2005	1196158
[pre- and postoperative radiotherapy of laryngeal cancer].		2005	1196026
[letter: adverse effects of antiepileptic drugs].		2005	1183340
[extraction of amitriptyline with organic solvents].		2005	1175818
hearing loss of acquired syphilis: diagnosis confirmed by incudectomy.	syphilis, both congenital and acquired, may cause sensori-neural hearing loss. congenital syphilis presents in two forms: early (infantile), and late (tardive). acquired syphilis, both secondary and tertiary, may cause deafness. the clinical course of the acquired and congenital forms are similar. sensori-neural hearing loss with low discrimination scores may affect both ears simultaneously or sequentially, and vestibular complaints are common. a clinical diagnosis can be made on the basis of hi ...	2005	1195972
[obstructive cardiomyopathy. a new surgical method of treatment].	a case of treatment of obstructive cardiomyopathy by insertion of a disc mistral prosthesis (cooley's technique) is reported. the result obtained was satisfactory from a clinical, haemodynamic and angiocardiographic standpoint. the mode of action of this technique is discussed.	2005	1168899
my years in local government.		2005	1111872
lactase insufficiency in chinese children detected by oral milk and lactose challenge.	a study to determine the incidence of late-onset lactose intolerance was undertaken in 68 normal chinese children in singapore using measurements of breath hydrogen concentrations, following a lactose or milk challenge, as indicator of lactose malabsorption. evidence of lactose malabsorption was detected in 26% of children given a lactose challenge, and in 21% of those given a milk challenge. it is concluded that late-onset lactase insufficiency is common in chinese children in singapore.	2005	2441643
[immunoblastic lymphadenopathy and toxacariasis. report of a case].	this is a report of a case a larval granulomatosis associated with immunoblastic lymphadenopathy, corroborated by laboratory and histologic studies compatible with that. the etiopathogenesis, clinical picture and diagnosis of both diseases are commented, and the possible relationship between them is stressed.	2005	1087413
[diagnosis of kartagener's syndrome].		2005	1081308
dna synthesis and turnover in the bullfrog tadpole during metamorphosis.	125i-labeled deoxyuridine (idurd) has been used to estimate the turnover of dna in liver, tail, and hind limb during spontaneous and triiodothyronine-induced metamorphosis. it was found that the total amount of liver dna remained constant and there was no significant loss of the label from the liver dna, which would be expected if there was an increase in dna turnover during metamorphosis. also, the change in specific activity of liver dna parallels that of tail dna during spontaneous metamorpho ...	2005	1084345
[lupins - a new source of food for andean countries. 5. traditional methods of debittering of lupins by water].	the bitter taste of lupin alkaloids can be sensorially detected in water within ppm-range. the strength of the taste diminishes as follows: sparteine, d-lupanin-perchlorate, lupinine, isolupinine and hydroxylupanine. the swelling capacity of lupin seeds presents different characteristics according to the species. the swelling rapidity is in l. albus somehow inferior to that of l. mutabilis. the absorption of water decreases in hot water, and the swelling rapidity increases. for domestic debitter ...	2005	556411
etiopathogenesis of degenerative joint diseases.		2005	1064949
dental treatment in the hemophiliac.		2005	1064933
[naevobasaliomatosis (gorlin-goltz syndrome)].		2005	551607
chromosomal aberrations defining uveal melanoma of poor prognosis.		2005	1347396
chromosomal aberrations defining uveal melanoma of poor prognosis.		2005	1347396
[diagnosis of bladder cancer by cytogenetic method].		2005	3437647
[diagnosis of bladder cancer by cytogenetic method].		2005	3437647
hazards of combined beta-blocker/diuretic tablets.		2005	2860426
determination of cerebral perfusion by means of planar brain scintigraphy and 99mtc-hmpao in brain death, persistent vegetative state and severe coma.	a total of 24 patients with clinical evidence of brain death (n = 17), severe coma (n = 2; gcs approximately 3) and apallic syndrome (n = 4) underwent a comparative investigation with 99mtc-hmpao brain scintigraphy, eeg, auditory and somatosensory evoked potentials. accompanied by eeg and evoked potentials, brain scintigraphy enabled confirmation of cerebral death in 15/17 patients. in one case clinical examination and evoked potentials suggest brain death, but cerebral perfusion and eeg were no ...	2005	1613202
ovulation induction and neural tube defects.		2005	1978153
neonatal tetanus: a developmental follow-up study.		2005	1278947
new algorithm to model protein-protein recognition based on surface complementarity. applications to antibody-antigen docking.	a novel algorithm is presented which models protein-protein interactions using surface complementarity. the method is applied to antibody-antigen docking. a steric scoring scheme, based upon a soft potential, is used to assess complementarity, and a simple electrostatic model is then used to remove infeasible interactions. the soft potential allows for structural changes that occur during docking. biochemical knowledge is necessary to reduce the number of docking orientations produced by the met ...	2005	1280302
potent block of cl- channels by antiallergic drugs.	two antiallergic drugs, cromolyn and ru 31156, were examined for their potency to block intermediate-conductance cl- channels. for this, single cl- channel activities of mucosal-type mast cells (rbl-2h3) and alternatively, of colonic carcinoma cells (ht29) were monitored employing the patch-clamp technique in the inside-out patch configuration. here we show that intermediate-conductance cl- channels of either cell type were blocked by both compounds. cl- channel inhibition occurred by a slow rat ...	2005	1280136
primary nerve repair in the upper limb. our preferred methods: theory and practical applications.	after years of controversy, it is now generally agreed that primary nerve repair by end-to-end coaptation, whenever feasible, yields better results than secondary procedures. we reviewed the theoretic basis of current methods of repair and described our preferred techniques and indications.	2005	1400607
letter: vaginal candidosis.		2005	1276796