Publications

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haematological effects of 2-phenoxyethanol and etomidate in carp (cyprinus carpio l.).to evaluate the effects of handling alone versus handling under anaesthesia with 2-phenoxyethanol or etomidate on haematological parameters in carp.201525545607
the in vitro immune modulatory effect of bisphenol a on fish macrophages via estrogen receptor α and nuclear factor-κb signaling.bisphenol a (bpa) is a well-known environmental endocrine-disrupting chemical. employing primary macrophages from head kidney of red common carp (cyprinus carpio), the present study aimed to evaluate the immune modulatory effect of bpa and to explore its potential action mechanism associated with estrogen receptor (er) and nuclear factor-κb (nf-κb) pathways. a dynamic response process was observed in macrophages upon various concentrations of bpa exposure, which significantly enhanced the antiba ...201525565130
fullerene-associated phenanthrene contributes to bioaccumulation but is not toxic to fish.the present study investigated the effects of aqueous fullerene suspensions (nc60 ) on the bioavailability and toxicity of phenanthrene (phe) to junior carp (cyprinus carpio). bioaccumulation factors (bafs) were calculated based on total as well as free concentrations of phe. equal baf values were obtained with and without nc60 based on the total concentrations, whereas greater bafs were found in the presence of nc60 when free phe concentrations were applied. the results demonstrated that nc60 c ...201525564991
copper exposure induces toxicity to the antioxidant system via the destruction of nrf2/are signaling and caspase-3-regulated dna damage in fish muscle: amelioration by myo-inositol.the muscle is the main portion of fish that is consumed by humans. copper (cu) can induce oxidative damage in fish muscle. however, the effects of cu exposure on the muscle antioxidant system and molecular patterns and preventive measures against these effects remain unclear. in this study, ros production, enzymatic and mrna levels of antioxidant enzymes and nf-e2-related factor 2 (nrf2) signaling-related molecules, antioxidant response element (are) binding ability, dna fragmentation and caspas ...201525562835
the effect of reactive oxygen species on motility parameters, dna integrity, tyrosine phosphorylation and phosphatase activity of common carp (cyprinus carpio l.) spermatozoa.the effect of reactive oxygen species production on the motility parameters, dna integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (cyprinus carpio) was investigated. spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (x-xo) either in the presence or absence of antioxidants for 15 and 60 min. a dose- and time-dependent reduction in spermatozoa motility and velocity was observed. comet assays showed a dr ...201525556750
assessment of pesticide residues and gene expression in common carp exposed to atrazine and chlorpyrifos: health risk assessments.this study assessed the impacts of atrazine (atr), chlorpyrifos (cpf) and combined atr/cpf exposure on the kidney of common carp (cyprinus carpio l.). the carp were sampled after a 40-d exposure to cpf and atr, individually or in combination, followed by a 40-d recovery to measure the expression levels of heat shock proteins genes (hsp60, hsp70 and hsp90) and pesticide residues in the kidney tissue. the results revealed that the mrna and protein levels of hsp60, hsp70 and hsp90 were induced in t ...201525568939
temporal dynamics of a local fish community are strongly affected by immigration from the surrounding metacommunity.a 5-year time series of annual censuses was collected from a large floodplain lake to determine how dynamics of the local fish community were affected by changes in hydrological connectivity with the surrounding metacommunity. the lake was disconnected from the metacommunity for 1 year prior to our study and remained disconnected until 3 months before our third annual census, when a flood reconnected the lake to the metacommunity. we determined how changes in connectivity affected temporal dynam ...201525628877
effect of heat treatment on the n-3/n-6 ratio and content of polyunsaturated fatty acids in fish tissues.the aim of this study was to compare the effect of different heat treatments (pan-frying, oven-baking, and grilling) on the contents of polyunsaturated fatty acids (pufas) in fish tissue. four fish species were examined: pike, carp, cod, and herring. high performance liquid chromatography, coupled with electrospray ionization and mass spectrometric detection (hplc/esi/ms), was employed for determination of intact lipid molecules containing n-3 and n-6 pufas. although mostly non-polar lipids (tri ...201525624225
preparation of nanoemulsions containing unsaturated fatty acid concentrate-chitosan capsules.fish oil is rich in unsaturated fatty acids (ω-3), which are highly susceptible to oxidative degradation. the use of encapsulation process to retard the fatty acid oxidation is an interesting alternative. in this work, nanoemulsions containing capsules of unsaturated fatty acids concentrate (ufac) using chitosan as wall material were prepared and characterized.201525617613
examining pyrethroids, carbamates and neonicotenoids in fish, water and sediments from the indus river for potential health risks.this 3 × 3 factorial study assessed pyrethroids, carbamates and neonicotenoids groups of pesticides in replicated samples of three fish species from low (s1, reference), medium (s2) and heavy (s3) polluted sites receiving agricultural run-offs around the indus river. water and sediment samples from the same sites were also analysed for these pesticides by using high-performance liquid chromatography. out of nine investigated pesticides, only three pesticides (deltamethrin, carbofuran and cyperme ...201525632902
differential effects of alloherpesvirus cyhv-3 and rhabdovirus svcv on apoptosis in fish cells.whilst herpesviridae, which infect higher vertebrates, actively influence host immune responses to ensure viral replication, it is mostly unknown if alloherpesviridae, which infect lower vertebrates, possess similar abilities. an important antiviral response is clearance of infected cells via apoptosis, which in mammals influences the outcome of infection. here, we utilise common carp infected with cyhv-3 to determine the effect on the expression of genes encoding apoptosis-related proteins (p53 ...201525596969
anti-carbamylated protein antibodies are present prior to rheumatoid arthritis and are associated with its future diagnosis.anti-carbamylated protein (anti-carp) antibodies could further elucidate early rheumatoid arthritis (ra) pathogenesis and predict clinical disease. we compared the diagnostic accuracy of anti-carp antibodies for future ra to other ra-related antibodies in military personnel.201525593232
chemical composition and immunomodulatory effects of enzymatic protein hydrolysates from common carp (cyprinus carpio) egg.the aim of this study was to prepare protein hydrolysates from underutilized common carp (cyprinus carpio) egg and to investigate their immunomodulatory effects in vivo.201525592018
oxidative stress and histopathological alterations in liver of cyprinus carpio l. induced by intraperitoneal injection of microcystin-lr.microcystins (mcs) are a group of cyclic heptapeptide hepatotoxic peptides produced by cyanobacteria. microcystins-lr (mc-lr) can inhibit the activities of protein phosphatase type 1 and type 2a (pp1 and pp2a) and induce excessive production of reactive oxygen species (ros). however, the detailed toxicological mechanism involving oxidative stress in carp (cyprinus carpio l.) remains largely unclear. in our present study, the effects of sublethal intraperitoneal doses of mc-lr on the oxidative st ...201525586193
profiling of skeletal muscle ankrd2 protein in human cardiac tissue and neonatal rat cardiomyocytes.muscle-specific mechanosensors ankrd2/arpp (ankyrin repeat protein 2) and ankrd1/carp (cardiac ankyrin repeat protein) have an important role in transcriptional regulation, myofibrillar assembly, cardiogenesis and myogenesis. in skeletal muscle myofibrils, ankrd2 has a structural role as a component of a titin associated stretch-sensing complex, while in the nucleus it exerts regulatory function as transcriptional co-factor. it is also involved in myogenic differentiation and coordination of myo ...201525585647
novel associations between contaminant body burdens and biomarkers of reproductive condition in male common carp along multiple gradients of contaminant exposure in lake mead national recreation area, usa.adult male common carp were sampled in 2007/08 over a full reproductive cycle at lake mead national recreation area. sites sampled included a stream dominated by treated wastewater effluent, a lake basin receiving the streamflow, an upstream lake basin (reference), and a site below hoover dam. individual body burdens for 252 contaminants were measured, and biological variables assessed included physiological [plasma vitellogenin (vtg), estradiol-17β (e2), 11-ketotestosterone (11kt)] and organ [g ...201525583583
chronic exposure to pollutants in madín reservoir (mexico) alters oxidative stress status and flesh quality in the common carp cyprinus carpio.madín reservoir (mr) is located on the río tlalnepantla in mexico. previous studies seeking to identify pollutants at this site evidence that mr water contains a considerable metal load as well as nonsteroidal anti-inflammatory drugs (nsaids) at concentrations above those determined suitable for aquatic life. this study aimed to evaluate whether chronic exposure to pollutants in mr alters oxidative stress status and flesh quality in muscle of the common carp cyprinus carpio. the following biomar ...201525583264
responses of the hepatic glutathione antioxidant defense system and related gene expression in juvenile common carp after chronic treatment with tributyltin.recently, residual organotin compounds have generally been recognised as relevant sources of aquatic environmental pollutants. however, the effects of these contaminants on the glutathione (gsh)-antioxidant system of fishes have not been adequately studied. in the current study, the chronic effects of tributyltin (tbt) found within antifouling paints for ships, on the gsh antioxidant system and related gene expression in the liver of juvenile common carp (cyprinus carpio) were investigated. fish ...201525582114
effects of nano-particles on histo-pathological changes of the fish.regarding fast development of the nanotechnology and the probably of it's side effects on aquatic body organs, this study investigate the effects of nanosilver administration on histology of gill, kidney and biochemical parameters in common carp. the silver nanoparticles were synthesized in a one-step reduction process in an aqueous solution. 60 o. mykiss were obtained from a local commercial hatchery. fish were divided randomly into four groups. control group was kept in dechlorinated tap water ...201526322234
mercury in sediment, water, and fish in a managed tropical wetland-lake ecosystem.mercury pollution has not been well documented in the inland lakes or fishes of mexico, despite the importance of freshwater fish as a source of protein in local diets. total mercury and methylmercury in waters, sediments, and the commercial fish catch were investigated in lake zapotlán, mexico. concentrations of total and methylmercury were very high in runoff and wastewater inputs, but very low in sediments and surface waters of the open water area of the lake. concentrations of total mercury ...201525909268
genetic connectivity and phenotypic plasticity in the cyprinodont aphanius farsicus from the maharlu basin, south-western iran.meristic and morphometric characteristics, including otolith data, of the farsi tooth-carp aphanius farsicus, which is endemic to the endorheic maharlu basin in south-western iran, were analysed for a sample of 92 individuals from four spring-streams; dna sequence data (cytochrome b gene) are presented for 29 specimens. some phenotypic variation was detected but the genetic data clearly indicate connectivity between the populations. possible links between phenotypic variation and environmental v ...201525644025
the resurrection of myxobolus toyamai with a validation of a stunted polar capsule based on morphological evidence.myxobolus toyamai has long been known as a gill parasite of the common carp cyprinus carpio in europe, asia and north america. recently, a controversy was raised regarding whether this organism was synonymous with thelohanellus toyamai because of ambiguity regarding the validity of the second stunted polar capsule. in the present study, detailed morphological examinations were conducted to reconfirm the presence of the small polar capsule of m. toyamai. the addition of potassium hydroxide or the ...201525640192
effects of cylindrospermopsin on the phagocytic cells of the common carp (cyprinus carpio l.).cylindrospermopsin is a cyanotoxin with cytotoxic activity. it is released into water during and after cyanobacterial water blooms and thus poses a threat to the health of fish. there is very little information available concerning the effects of the toxin on fish immune cells. in this study, we assessed the potential impact of cylindrospermopsin on the basic functions of phagocytic cells from common carp (cyprinus carpio l.), including phagocytosis, reactive oxygen and nitrogen species producti ...201525639895
anti-inflammatory and hepatoprotective effects of ganoderma lucidum polysaccharides on carbon tetrachloride-induced hepatocyte damage in common carp (cyprinus carpio l.).the aim of this study was to investigate the anti-inflammatory and hepatoprotective effects of ganoderma lucidum polysaccharides (glps) on carbon tetrachloride (ccl4)-induced hepatocyte damage in common carp (cyprinus carpio l.). glps (0.1, 0.3, 0.6mg/ml) were added to the primary hepatocytes before (pre-treatment), after (post-treatment) and both before and after (pre- and post-treatment) the incubation of the hepatocytes with ccl4 at the concentration of 8mm in the culture medium. the supernat ...201525639226
reorganization of low-molecular-weight fraction of plasma proteins in the annual cycle of cyprinidae.reorganization of the low-molecular-weight fraction of cyprinid plasma was analyzed using various electrophoretic techniques (disc electrophoresis, electrophoresis in polyacrylamide concentration gradient, in polyacrylamide with urea, and in sds-polyacrylamide). the study revealed coordinated changes in the low-molecular-weight protein fractions with seasonal dynamics and related reproductive rhythms of fishes. we used cultured species of the cyprinidae family with sequenced genomes for the dete ...201525756535
effects of prometryne on early life stages of common carp (cyprinus carpio l.).toxicity of prometryne to early life stages of common carp was assessed. on the basis of accumulated mortality in the experimental groups lowest observed-effect concentration (loec) was estimated as 1100 µg/l; and no observed-effect concentration (noec) was 850 µg/l. fulton's condition factor was significantly lower than in controls in fish exposed to 4000 µg/l after 7, 14, and 21 days. by day 14, fish exposed to 4000 µg/l prometryne showed significantly lower mass and total length compared to c ...201525752431
correction: the muscle ankyrin repeat proteins carp, ankrd2, and darp are not essential for normal cardiac development and function at basal conditions and in response to pressure overload. 201525747160
alteration in the cytokine levels and histopathological damage in common carp induced by glyphosate.glyphosate is one of the most frequently used herbicides, and it has been demonstrated to generate a series of toxicological problems in animals and humans. however, relatively little is known about the effects of glyphosate on the immune system of fish. in the present study, the acute toxicity of glyphosate on common carp was first determined; then, the contents of interferon-γ (ifn-γ), interleukin-1β (il-1β), and tumor necrosis factor -α (tnf-α) and histopathological alterations in the liver, ...201525747155
optimization of demineralization on cyprinus carpio haematopterus scale by response surface methodology.fish scale, separated during fish mechanical processing, can serve as an additional source of proteins, especially of collagen proteins. to obtain high purity native collagen, it is required to carry out deproteinization and demineralization of fish scales. therefore, the aim of this study was to determine the optimal conditions for demineralization of cyprinus carpio haematopterus scale without loss of collagen content in hcl solution. here, the demineralization of cyprinus carpio haematopterus ...201525745241
effect of blend ratio and ph on the physical properties of edible composite films prepared from silver carp surimi and skin gelatin.the effect of blend ratio and ph on the physical properties of surimi-gelatin composite films was investigated. tensile strength (ts), film water solubility and soluble proteins of composite films increased with the increasing proportion of gelatin, while elongation at break (eab) decreased. the ts of neutral films with the same ratio of surimi and gelatin were lowest, while increased at acidic or alkaline conditions. similar tendency was also found in protein solubility and surface hydrophobici ...201525745232
study on heavy metal levels and its health risk assessment in some edible fishes from nansi lake, china.eight heavy metals, namely cu, zn, fe, mn, cd, ni, pb, and as in the muscles of nine fish species collected from nansi lake, china. were determined, and the potential health risks to local residents via consumption of the fishes were estimated. the results of two-way anova that showed the concentrations of heavy metals in the investigated fish samples were influenced significantly by fish species and sampling sites. correlation analysis indicated that sampling sites had significant effects on th ...201525740685
polymorphism analysis of the intron one of insulin-like growth factor 2 receptor gene (igf2r) in ffrc strain common carp (cyprinus carpio l.) and its relationship with growth performance.the insulin-like growth factor 2 receptor gene (igf2r) encodes a transmembrane protein receptor and acts to sequester and degrade excess circulating insulin-like growth factor 2, which is critical for normal mammalian growth and development. thus, igf2r may serve as a candidate gene underlying growth trait in the common carp. in this study, we isolated the intron one of common carp igf2r and detected the diversity in 3 continuous generations of ffrc strain common carp. a total of 8 loci were det ...201525729973
cue-based and algorithmic learning in common carp: a possible link to stress coping style.common carp that had been screened for stress coping style using a standard behavioural test (response to a novel environment) were given a learning task in which food was concealed in one of two compartments, its location randomised between trials and its presence in a given compartment signalled by either a red or a yellow light. all the fish learned to find food quickly, but did so in different ways. fifty five percent learned to use the light cue to locate food; the remainder achieved the sa ...201525725347
[investigation of membrane permeability of carp spermatozoa for water molecules].the fundamentals of a photometry method for determination of membrane permeability of some fish spermatozoa for water molecules are presented. osmotic tolerance of carp spermatozoa membranes was studied using epr-spectroscopy and photometric analysis methods. it was shown that carp spermatozoa look like the ideal osmometers in their reaction on media of different osmolarity. the value of membrane permeability of carp spermatozoa for water molecules was determined. data obtained can be used in cr ...201525715589
phosphorylation-independent suppression of light-activated visual pigment by arrestin in carp rods and cones.visual pigment in photoreceptors is activated by light. activated visual pigment (r*) is believed to be inactivated by phosphorylation of r* with subsequent binding of arrestin. there are two types of photoreceptors, rods and cones, in the vertebrate retina, and they express different subtypes of arrestin, rod and cone type. to understand the difference in the function between rod- and cone-type arrestin, we first identified the subtype of arrestins expressed in rods and cones in carp retina. we ...201525713141
mitochondrial genome sequencing and development of genetic markers for the detection of dna of invasive bighead and silver carp (hypophthalmichthys nobilis and h. molitrix) in environmental water samples from the united states.invasive asian bighead and silver carp (hypophthalmichthys nobilis and h. molitrix) pose a substantial threat to north american aquatic ecosystems. recently, environmental dna (edna), genetic material shed by organisms into their environment that can be detected by non-invasive sampling strategies and genetic assays, has gained recognition as a tool for tracking the invasion front of these species toward the great lakes. the goal of this study was to develop new species-specific conventional pcr ...201525706532
bioconcentration and trophic transfer of polychlorinated biphenyls and polychlorinated dibenzo-p-dioxins and dibenzofurans in aquatic animals from an e-waste dismantling area in east china.eight aquatic biota species were collected from an e-waste dismantling area in east china to investigate bioconcentration and trophic transfer of polychlorinated biphenyls (pcbs), polychlorinated dibenzo-p-dioxins (pcdds) and polychlorinated dibenzofurans (pcdfs). the mean concentrations of pcbs varied widely from 6.01 × 10(4) to 2.27 × 10(6) pg per g dry weight (dw). the ∑25pcb concentrations in eels were significantly higher than those in other species. the levels of pcdd/fs changed from 8.13 ...201525689167
effect of dietary glutamine on growth performance, non-specific immunity, expression of cytokine genes, phosphorylation of target of rapamycin (tor), and anti-oxidative system in spleen and head kidney of jian carp (cyprinus carpio var. jian).this study was designed to investigate the effects of dietary glutamine on the growth performance, cytokines, target of rapamycin (tor), and antioxidant-related parameters in the spleen and head kidney of juvenile jian carp (cyprinus carpio var. jian). fish were fed the basal (control) and glutamine-supplemented (12.0 g glutamine kg(-1) diet) diets for 6 weeks. results indicated that the dietary glutamine supplementation improved the growth performance, spleen protein content, serum complement 3 ...201525675866
effect of cholesterol-loaded cyclodextrin on cryosurvival and fertility of cryopreserved carp (cyprinus carpio) sperm.addition of cholesterol-loaded cyclodextrin (clc) to the diluents of mammalian semen increased stability and rigidity of phospholipid hydrocarbon chains of plasma membrane during sperm cryopreservation process. clc has been tested successfully as cryoprotectant in various livestock sperm cryopreservation protocols but its efficacy for cryopreserving of fish sperm has not previously been tested. in the present study, different cholesterol loaded cyclodextrin concentrations were evaluated for the ...201525661710
immunomodulatory effect of arsenic on cytokine and hsp gene expression in labeo rohita fingerlings.immune system is fundamental for survival of an organism against invading pathogens and other harmful agents. cytokines, the signaling proteins that are produced transiently after cell activation and exert pleiotropic effects on cells of the immune system, are important mediators of cell mediated immune response. when expressed in a dysregulated fashion cytokines can underlie either immunodeficient or immunopathologic states. heat shock proteins (stress proteins, hsps) are also key proteins, whi ...201525652292
effects of atrazine and chlorpyrifos on oxidative stress-induced autophagy in the immune organs of common carp (cyprinus carpio l.).atrazine (atr) and chlorpyrifos (cpf) are the most common agrochemical in the freshwater ecosystems of the world. this study assessed the effects of atr (4.28, 42.8 and 428 μg/l), cpf (1.16, 11.6 and 116 μg/l) and combined atr/cpf (1.13, 11.3 and 113 μg/l) on common carp head kidneys and spleens following 40 d exposure and 40 d recovery treatments. nitric oxide (no) content, activities of anti hydroxyl radical (ahr), anti superoxide anion (asa), peroxidase (pod) and inducible nitric oxide syntha ...201525652291
a role for melatonin in maintaining the pro- and anti-inflammatory balance by influencing leukocyte migration and apoptosis in carp.melatonin is responsible for the synchronization of many physiological processes, including the immune response. here we focus on the expression of melatonin mt1 receptors in/on leukocytes, and on the effects of melatonin administration on the inflammatory processes of carp. for the first time, we showed that fish leukocytes express mt1 receptors, implicating direct responsiveness to melatonin stimulation. moreover, both in vitro and in vivo, melatonin modulated the immune response. the most pot ...201526188098
effect of salicylic acid on early life stages of common carp (cyprinus carpio).environmental concentrations of pharmaceutical residues are often low; nevertheless, they are designed to have biological effects at low doses. the aim of this study was to assess the effects of salicylic acid on the growth and development of common carp (cyprinus carpio) early life stages with respect to antioxidant defence enzymes. an embryo-larval toxicity test lasting 34 days was performed according to oecd guidelines 210 (fish, early-life stage toxicity test). the tested concentrations were ...201526183809
preliminary genetic linkage map of indian major carp, labeo rohita (hamilton 1822) based on microsatellite markers.linkage map with wide marker coverage is an essential resource for genetic improvement study for any species. sex-averaged genetic linkage map of labeo rohita, popularly known as 'rohu', widely cultured in the indian subcontinent, was developed by placing 68 microsatellite markers generated by a simplified method. the parents and their f1 progeny (92 individuals) were used as segregating populations. the genetic linkage map spans a sex-averaged total length of 1462.2 cm, in 25 linkage groups. th ...201526174674
characterization of igm-binding protein: a pigr-like molecule expressed by intestinal epithelial cells in the common carp (cyprinus carpio l.).the adaptive mucosal immune system seems to be an important defence mechanism for fish, but the binding of immunoglobulin m (igm) in mucosal organs has yet to be clarified in fish. the present study was designed to search for the protein that binds igm in the intestinal epithelium and determine its distribution in mucosa-associated lymphoid tissues of the common carp (cyprinus carpio l.). the serum-derived carp igm fraction was isolated by sephadex g-200 and assessed for purity by sds-page under ...201526166176
effects of different spawning agents on serum levels of reproductive steroid hormones and cortisol level in adult female barbus sharpeyi (gunther, 1874).the question of whether, as hormone therapies, spawning agents differ from each other to induce physiological pathways of gametogenesis and oocyte maturation in fish remains important, because it could modify undesirable changes, regulated by endocrine systems of individual fish. a series of experimental treatments were applied to investigate the underlying mechanism(s) in which female bunnei (barbus sharpeyi) fish respond differently to hormone therapies. female broodstocks were injected twice ...201526164861
rheumatoid arthritis-associated autoantibodies in non-rheumatoid arthritis patients with mucosal inflammation: a case-control study.rheumatoid arthritis-associated autoantibodies (ra-aab) can be present in serum years before clinical onset of rheumatoid arthritis (ra). it has been hypothesized that initiation of ra-aab generation occurs at inflamed mucosal surfaces, such as in the oral cavity or lungs. the aim of this study was to assess systemic presence of ra-aab in patients without ra who had oral or lung mucosal inflammation.201526155788
construction, de-novo assembly and analysis of transcriptome for identification of reproduction-related genes and pathways from rohu, labeo rohita (hamilton).rohu is a leading candidate species for freshwater aquaculture in south-east asia. unlike common carp the monsoon breeding habit of rohu restricts its seed production beyond season indicating strong genetic control over spawning. genetic information is limited in this regard. the problem is exacerbated by the lack of genomic-resources. we identified 182 reproduction-related genes previously by sanger-sequencing which were less to address the issue of seasonal spawning behaviour of this important ...201526148098
neuroanatomical localization of nitric oxide synthase (nnos) in the central nervous system of carp, labeo rohita during post-embryonic development.nitric oxide (no) is a chemically diffusible molecular messenger playing various roles in both vertebrates and invertebrates. nitric oxide synthase (nos) is the key enzyme in synthesis of no. the neuroanatomical distribution pattern of neuronal nitric oxide synthase (nnos) was studied and developing stages of labeo rohita such as hatchlings (10-15mm), frys (15-35mm), semi-fingerlings (35-65mm), fingerlings (65-100mm) and adults (350-370mm) were used. in the telencephalon, nitrergic cells were ob ...201526143285
dexamethasone action on caudal fin regeneration of carp cyprinus carpio (linnaeus, 1758).studies have demonstrated that the prolonged use of corticoids can delay the healing process, affecting re-epithelialization, neovascularization and collagen synthesis. as the fins of teleost fish contain a large amount of collagen, the aim of the present study was to investigate the effect of dexamethasone (anti-inflammatory and glucocorticoid steroid widely used in the treatment of rheumatic diseases) during the regeneration process in the caudal fin of specimens of carp (cyprinus carpio). for ...201526132030
diversification of the duplicated rab1a genes in a hypoxia-tolerant fish, common carp (cyprinus carpio).common carp is a widely cultivated fish with longer than 2,000 years domestication history, due to its strong environmental adaptabilities, especially hypoxia tolerance. the common carp genome has experienced a very recent whole genome duplication (wgd) event. among a large number of highly similar duplicated genes, a pair of ras-associated binding-gtpase 1a (rab1a) genes were found fast diverging. four analogous rab1a genes were identified in the common carp genome. comparisons of gene structur ...201526129846
proteomic analysis of extracellular medium of cryopreserved carp (cyprinus carpio l.) semen.during freezing and thawing, spermatozoa are exposed to physical and chemical stressors that result in adverse changes in sperm structures and physiological functions. the present study provides, for the first time, a comprehensive description of protein changes in the extracellular medium of cryopreserved semen. using 2d-dige and a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography electrospray ionization tandem mass spectromet ...201526125334
increased acute toxicity to fish caused by pharmaceuticals in hospital effluents in a pharmaceutical mixture and after solar irradiation.hospital effluents are an important source of residual drugs and other classes of pharmaceuticals in aquatic environments. the raw wastewater from the studied hospital exhibited acute toxicity to vertebrate organisms, and cyprinus carpio was the most sensitive organism tested. a mixture of 19 commonly used pharmaceuticals caused acute toxicity to c. carpio with an lc50 value of 60.68mgl(-1) after 96h. this study demonstrated that irradiation for 1-5days significantly increased the acute toxicity ...201526121604
shared segment analysis and next-generation sequencing implicates the retinoic acid signaling pathway in total anomalous pulmonary venous return (tapvr).most isolated congenital heart defects are thought to be sporadic and are often ascribed to multifactorial mechanisms with poorly understood genetics. total anomalous pulmonary venous return (tapvr) occurs in 1 in 15,000 live-born infants and occurs either in isolation or as part of a syndrome involving aberrant left-right development. previously, we reported causative links between tavpr and the pdgfra gene. tapvr has also been linked to the ankrd1/carp genes. however, these genes only explain ...201526121141
transcriptome profiling analysis of naked carp (gymnocypris przewalskii) provides insights into the immune-related genes in highland fish.the naked carp, gymnocypris przewalskii, is one of the dominant aquaculture fish species in qinghai province, china. its wild stocks have severely suffered from overfishing, and the farming species are vulnerable to various pathogens infections. here we report the first immune-related tissues transcriptome of a wild naked carp using a deep sequencing approach. a total of 158,087 unigenes are generated, 2687 gill-specific gene and 3215 kidney-specific genes are identified, respectively. gene onto ...201526117731
exploring erythropoiesis of common carp (cyprinus carpio) using an in vitro colony assay in the presence of recombinant carp kit ligand a and erythropoietin.the use of in vitro colony assays in mammals has contributed to identification of erythroid progenitor cells such as burst-forming unit-erythroid (bfu-e) and colony-forming unit-erythroid (cfu-e) progenitors, and serves to examine functions of erythropoietic growth factors like erythropoietin (epo) and kit ligand. here, we established an in vitro colony-forming assay capable of investigating erythropoiesis in carp (cyprinus carpio), cloned and functionally characterized recombinant homologous mo ...201526111997
effects of nitric oxide on blood-brain barrier permeability in common carp (cyprinus carpio l.).to determine in vivo effects of nitric oxide (no) on blood-brain barrier (bbb) permeability in common carp (cyprinus carpio l.).201526111091
yy super sperm lead to all male triploids and tetraploids.androgenesis is a unique and rarely encountered reproductive mode in which the offspring only inherit the paternal nuclear genome, resulting in relatively few viable individuals.201526108606
ca(2+) and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of jian carp (cyprinus carpio var. jian).there are young erythrocytes and mature erythrocytes in the peripheral blood of fish. the present study explored the apoptosis in hydroxyl radical ((·)oh)-induced young and mature erythrocytes of jian carp (cyprinus carpio var. jian). carp erythrocytes from the peripheral blood were separated into the young fraction, the intermediate fraction and the mature fraction using fixed-angle centrifugation. the erythrocytes in three age fractions were treated with the caspase inhibitors (zvad-fmk) in ph ...201526080678
complex responses to si quantum dots accumulation in carp liver tissue: beyond oxidative stress.the use of quantum dots (qds) in biomedical applications is limited due to their inherent toxicity caused by the heavy metal core of the particles. consequently, silicon-based qds are expected to display diminished toxicity. we investigated the in vivo effects induced by si/sio2 qds intraperitoneally injected in crucian carp liver. the qds contained a crystalline si core encased in a sio2 shell, with a size between 2.75 and 11.25nm and possess intrinsic fluorescence (ex 325nm/em ∼690nm). tissue ...201526079203
assessing joint toxicity of four organophosphate and carbamate insecticides in common carp (cyprinus carpio) using acetylcholinesterase activity as an endpoint.mixtures of organophosphate (op) and carbamate (cb) pesticides are commonly detected in freshwater ecosystems. these pesticides inhibit the activity of acetylcholinesterase (ache) and have potential to interfere with behaviors that may be essential for the survival of species. although the effects of individual anticholinesterase insecticides on aquatic species have been studied for decades, the neurotoxicity of mixtures is still poorly understood. in the present study, brain ache inhibition in ...201526071811
toxicity and molecular effects of di-n-butyl phthalate (dbp) on cyp1a, sod, and gpx in cyprinus carpio (common carp).di-n-butyl phthalate (dbp), a widely used plasticizer in the plastic industry, affects regulation of the endocrine system and causes toxicity in animals. in the present study, we evaluated a series of ecotoxicological stress biomarkers in the common carp (cyprinus carpio) as an experimental model to test for alterations in gene expression at a sublethal concentration of 1 mg/l dbp for 4, 24, and 96 h. in gills, an immediate increase in cyp1a messenger rna (mrna) levels was observed within the fi ...201526065888
prevalence of ectoparasites in carp fry and fingerlings of rajshahi district, bangladesh.the study has been carried out to find out the prevalence of ectoparasites of carp fry and fingerlings during june 2010 to may 2011 from rajshahi district, bangladesh. four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from 560 species of endemic and exotic carps collected from rajshahi district, bangladesh. the highest ectoparasitic prevalence (37.5 %) has been recorded for ciliophoran group in winter and the lowest prevalence in crustacean (5 %) in ...201526063985
phylogeny and evolution of multiple common carp (cyprinus carpio l.) populations clarified by phylogenetic analysis based on complete mitochondrial genomes.common carp (cyprinus carpio l.) is one of the oldest, most widely farmed commercially important freshwater fish in the world. however, many undetermined phylogenetic relationships and origins of common carp lineages remain, which are obstacles to conservation and genetic breeding of this species. phylogenetic analyses based on molecular tools are helpful to distinguish the origin of species, understand and clarify their evolutionary history, and provide a genetic basis for selective breeding. i ...201526054828
in vivo and in vitro isomer-specific biotransformation of perfluorooctane sulfonamide in common carp (cyprinus carpio).biotransformation of pfos-precursors (prefos) may contribute significantly to the level of perfluorooctanesulfonate (pfos) in the environment. perfluorooctane sulfonamide (pfosa) is one of the major intermediates of higher molecular weight prefos. its further degradation to pfos could be isomer specific and thereby explain unexpected high percentages of branched (br-) pfos isomers observed in wildlife. in this study, isomeric degradation of pfosa was concomitantly investigated by in vivo and in ...201526053759
activity of the hypothalamus-pituitary-interrenal axis (hpi axis) and immune response in carp lines with different susceptibility to disease.the stress response transmitted by the hpa axis is one of the best examples of neuroendocrine-immune interactions that are critical for survival. analogous to the situation in mammals, the stress response in fish is characterized by the activation of the hypothalamo-pituitary-interrenal axis (hpi). effects of cortisol on the fish immune system comply with findings in mammals and suggest that the differences in sensitivity to stress will influence the immune response and as a consequence of survi ...201526041250
biochemical responses over time in common carp cyprinus carpio (teleostei, cyprinidae) during fed supplementation with α-lipoic acid.the current study aimed to evaluate the influence of lipoic acid (la) supplementation (439.84±6.71 mg la/kg feed) on antioxidants responses throughout the time in intestine, liver and muscle of juvenile common carp cyprinus carpio. two experimental groups were fed during four weeks with a diet with or without la. glutathione-s-transferase (gst) activity, glutathione (gsh) content, antioxidant capacity against peroxyl radicals (acap) and lipid peroxidation (tbars) were evaluated in these organs. ...201526037328
trichlorfon-induced haematological and biochemical changes in cyprinus carpio: ameliorative effect of propolis.trichlorfon is among the most commonly used products to treat fish parasites in aquaculture. we investigated the effectiveness of propolis in alleviating the toxicity of trichlorfon on haematological and oxidant/antioxidant parameters in carp cyprinus carpio. fish were exposed to sublethal concentrations (11 and 22 mg l-1) of trichlorfon, and propolis (10 mg kg-1 of fish weight) was simultaneously administered. at the end of 14 d administration, blood and tissue (liver, kidney, gill) samples wer ...201526036828
risk factors for development of internal neoplasms in koi carp cyprinus carpio koi.fish, like mammals, can be affected by neoplastic proliferations. as yet, there are only a very small number of studies reporting on the occurrence of tumours in koi carp cyprinus carpio koi and only sporadic reports on the nature of the tumours or on risk factors associated with their development. between 2008 and 2012, koi with abdominal swelling were examined pathologically: neoplastic lesions were diagnosed and classified histologically. we evaluated possible risk factors for the development ...201526036827
interactions between water temperature and contaminant toxicity to freshwater fish.warming of freshwaters as a result of climate change is expected to have complex interactions with the toxicity of contaminants to aquatic organisms. the present study evaluated the effects of temperature on the acute toxicity of endosulfan, chlorpyrifos, and phenol to 3 warm water species of fish-silver perch, rainbowfish, and western carp gudgeon-and 1 cold water species, rainbow trout. endosulfan was more toxic to silver perch at 30 °c and 35 °c than at 15 °c, 20 °c and 25 °c during short exp ...201526033197
[the bipolarity of the genetic structure of communities of the crucian carp (carassius linnaeus, 1758) as a reflection of paradoxal reproductive relationships].an analysis of 133 populations of carasiius spp. from water bodies in ukraine showed their polyspecific structure: 62% of individuals of the studied 3453 specimens. turned out to be bisexual goldfishes c. auratus, 25%. were unisexual prissian carps c. gibelio, 6% accounted for the aboriginal species crucian carp c. carassius, and 6% were hybrids c. auratus x c. carassius. in this case a clear trend has been revealed towards the formation of homogeneous alternative populations, specifically: eith ...201526030976
nsaid-manufacturing plant effluent induces geno- and cytotoxicity in common carp (cyprinus carpio).the pharmaceutical industry generates wastewater discharges of varying characteristics and contaminant concentrations depending on the nature of the production process. the main chemicals present in these effluents are solvents, detergents, disinfectants - such as sodium hypochlorite (naclo) - and pharmaceutical products, all of which are potentially ecotoxic. therefore, this study aimed to evaluate the geno- and cytotoxicity induced in the common carp cyprinus carpio by the effluent emanating f ...201526026403
genome-wide identification, evolution and expression analysis of nuclear receptor superfamily in nile tilapia, oreochromis niloticus.the nuclear receptor (nr) superfamily, which is divided into 7 subfamilies, constitutes one of the largest classes of transcription factors. in this study, through comprehensive database search, we identified all nrs (including 4 novel members) from the tilapia (75), common carp (137), zebrafish (73), fugu (73), tetraodon (72), stickleback (70), medaka (69), coelacanth (55), spotted gar (51) and elephant shark (50). for 21 nrs, two duplicates were found in teleosts, while only one in tetrapods. ...201526024593
effects of glutamate on growth, antioxidant capacity, and antioxidant-related signaling molecule expression in primary cultures of fish enterocytes.the present study explored the effects of glutamate (glu) on the growth, antioxidant capacity, and gene expression of nf-e2-related nuclear factor 2 (nrf2) signaling molecule in enterocytes of jian carp (cyprinus carpio var. jian). the enterocytes were incubated in media containing 0, 2, 4, 6, 8, and 10 mm/l glu for 96 h. the results showed that glu could promote fish enterocytes proliferation and differentiation. additionally, activities of alkaline phosphatase, na(+), k(+)-atpase, γ-glutamyl t ...201525999304
compression anastomotic ring-locking procedure (carp) is a safe and effective method for intestinal anastomoses following left-sided colonic resection.compression anastomotic ring-locking procedure (carp) is a novel procedure for creating colonic anastomoses. the surgical procedure allows perioperative quantification of the compression pressure between the intestinal ends within the anastomosis and postoperative monitoring of the anastomotic integrity. we have recently shown that carp is a safe and effective method for colonic anastomoses in pigs, and the purpose of the present study was to evaluate carp for colonic anastomoses in humans.201525989929
acute and subchronic effects on immune responses of carp (cyprinus carpio l.) after exposure to deoxynivalenol (don) in feed.the mycotoxin deoxynivalenol (don) has been shown to regularly occur at relevant concentrations in feed designed for aquaculture use, but little is known about the consequences of its presence on the organisms that consume the don-contaminated feed. previous studies indicated a down-regulation of pro-inflammatory responses in carp (cyprinus carpio l.) after 4 weeks of feeding don. the present study examined the time course of innate immune responses of carp to orally administered don. changes in ...201525989849
carp thrombocyte phagocytosis requires activation factors secreted from other leukocytes.thrombocytes are nucleated blood cells in non-mammalian vertebrates, which were recently focused on not only as hemostatic cells but also as immune cells with potent phagocytic activities. we have analyzed the phagocytic activation mechanisms in common carp (cyprinus carpio) thrombocytes. macs-sorted mab(+) thrombocytes showed no phagocytic activity even in the presence of several stimulants. however, remixing these thrombocytes with other anti-thrombocyte mab(-) leukocyte populations restored t ...201525978929
environmental degradation in a eutrophic shallow lake is not simply due to abundance of non-native cyprinus carpio.non-native species are often major drivers of the deterioration of natural ecosystems. the common carp cyprinus carpio are known to cause major changes in lentic systems, but may not be solely responsible for large scale changes in these ecosystems. we used data from extensive collection efforts to gain insight into the importance of carp as drivers of ecosystem change in lake patzcuaro, mexico. we compared the structure (fish density, biomass, diversity, and evenness) of fish assemblages from s ...201525975439
metabolic disposition and elimination of cyadox in pigs, chickens, carp, and rats.the metabolism, distribution, and elimination of cyadox (cya) is investigated in pigs, chickens, carp, and rats to identify the marker residue and target tissue of cya in food animals for food safety concerns. following a single oral gavage of [(3)h]-cya, the total radioactivity was rapidly excreted, with more than 95% of the dose excreted within 14 days in the four species. fecal excretion of the total radioactivity was 66.2% and 51.6%, and urinary excretion of the total radioactivity was 28.35 ...201525973850
genetic analysis of qtl for eye cross and eye diameter in common carp (cyprinus carpio l.) using microsatellites and snps.a group of 107 f1 hybrid common carp was used to construct a linkage map using joinmap 4.0. a total of 4877 microsatellite and single nucleotide polymorphism (snp) markers isolated from a genomic library (978 microsatellite and 3899 snp markers) were assigned to construct the genetic map, which comprised 50 linkage groups. the total length of the linkage map for the common carp was 4775.90 cm with an average distance between markers of 0.98 cm. ten quantitative trait loci (qtl) were associated w ...201525966124
distribution and age-related bioaccumulation of lead (pb), mercury (hg), cadmium (cd), and arsenic (as) in tissues of common carp (cyprinus carpio) and european catfish (sylurus glanis) from the buško blato reservoir (bosnia and herzegovina).the purpose of this study was to quantify the bioaccumulation of pb, hg, cd, and as in tissues of carp (cyprinus carpio) and catfish (silurus glanis) from buško blato in bosnia and herzegovina. arsenic concentrations were below the maximal admissible concentration (mac) for croatia and other countries. mercury concentrations were below 1 mg kg(-1), but in most muscle samples of both species and all catfish liver samples, the values were higher than 0.5 mg kg(-1) (higher than the mac for many cou ...201525966047
effect of cacl2 on denaturation and aggregation of silver carp myosin during setting.the effect of cacl2 on denaturation and aggregation of silver carp myosin incubated at 40 °c was investigated by circular dichroism spectroscopy, surface hydrophobicity (s0-ans), total sulfhydryl (sh) group content, zeta potential, turbidity, z-average diameter (dz), and dynamic rheological analysis. during setting at 40 °c, both cacl2 and heating induced conformational changes of the fish myosin, and exposure of more hydrophobic amino acid residues and free sh groups, followed by myosin aggrega ...201525952860
three-dimensional structure and ligand-binding site of carp fishelectin (fel).carp fel (fishelectin or fish-egg lectin) is a 238-amino-acid lectin that can be purified from fish eggs by exploiting its selective binding to sepharose followed by elution with n-acetylglucosamine. its amino-acid sequence and other biochemical properties have previously been reported. the glycoprotein has four disulfide bridges and the structure of the oligosaccharides linked to asn27 has been described. here, the three-dimensional structures of apo carp fel (cfel) and of its complex with n-ac ...201525945578
local adaptation of gymnocypris przewalskii (cyprinidae) on the tibetan plateau.divergent selection among environments affects species distributions and can lead to speciation. in this article, we investigated the transcriptomes of two ecotypes of scaleless carp (gymnocypris przewalskii przewalskii and g. p. ganzihonensis) from the tibetan plateau. we used a transcriptome sequencing approach to screen approximately 250,000 expressed sequence tags (ests) from the gill and kidney tissues of twelve individuals from the ganzi river and lake qinghai to understand how this freshw ...201525944748
relationships between climate and growth of gymnocypris selincuoensis in the tibetan plateau.the consequences of climate change are becoming increasingly evident in the tibetan plateau, represented by glaciers retreating and lakes expanding, but the biological response to climate change by plateau-lake ecosystems is poorly known. in this study, we applied dendrochronology methods to develop a growth index chronology with otolith increment widths of selincuo naked carp (gymnocypris selincuoensis), which is an endemic species in lake selincuo (4530 m), and investigated the relationships b ...201525937912
local and systemic adaptive immune responses toward viral infection via gills in ginbuna crucian carp.recent studies on fish immunity highlighted the significance of gills as mucosal immune tissues. to understand potential of gills as vaccination sites for inducing adaptive systemic immunity, we investigated virus-specific cell-mediated and humoral immune responses following a "per-gill infection method", which directly exposes virus only to gills. the viral load in crucian carp hematopoietic necrosis virus (chnv)-infected gills decreased after peaking at a particular time point. furthermore, th ...201525936589
scales tell a story on the stress history of fish.fish faced with stressful stimuli launch an endocrine stress response through activation of the hypothalamic-pituitary-interrenal (hpi-) axis to release cortisol into the blood. scientifically validated biomarkers to capture systemic cortisol exposure over longer periods of time are of utmost importance to assess chronic stress in governmental, wildlife, aquaculture and scientific settings. here we demonstrate that cortisol in scales of common carp (cyprinus carpio l.) is the long-sought biomark ...201525922947
sodium cyanide induced alteration in the whole animal oxygen consumption and behavioural pattern of freshwater fish labeo rohita.sodium cyanide is a common environmental pollutant which is mainly used in many industries such as mining, electroplating, steel manufacturing, pharmaceutical production and other specialized applications including dyes and agricultural products. it enters aquatic environment through effluents from these industries. static renewal bioassay test has been conducted to determine lc, of sodium cyanide on indigenous freshwater carp, labeo rohita. the behavioural pattern and oxygen consumption were ob ...201525895263
carp-1/ccar1: a biphasic regulator of cancer cell growth and apoptosis.targeted cancer therapy using small molecule inhibitors (smis) has been useful in targeting the tumor cells while sparing the normal cells. despite clinical success of many targeted therapies, their off-target effects and development of resistance are emerging as significant and challenging problems. thus, there is an urgent need to identify targets to devise new means to treat cancers and their drug-resistant phenotypes. carp-1/ccar1 (cell division cycle and apoptosis regulator 1), a peri-nucle ...201525894788
anti-carbamylated protein antibodies in the pre-symptomatic phase of rheumatoid arthritis, their relationship with multiple anti-citrulline peptide antibodies and association with radiological damage.the presence of a new autoantibody system, anti-carbamylated protein (anti-carp) antibodies, has been identified in rheumatoid arthritis (ra). the presence of anti-carp antibodies was evaluated in samples taken from individuals who subsequently developed ra before and after onset of symptoms and related to previously analysed antibodies against citrullinated peptides (acpa specificities) and anti-ccp2.201525889922
native and non-native plants provide similar refuge to invertebrate prey, but less than artificial plants.non-native species introductions are widespread and can affect ecosystem functioning by altering the structure of food webs. invading plants often modify habitat structure, which may affect the suitability of vegetation as refuge and could thus impact predator-prey dynamics. yet little is known about how the replacement of native by non-native vegetation affects predator-prey dynamics. we hypothesize that plant refuge provisioning depends on (1) the plant's native status, (2) plant structural co ...201525885967
temperature and species-specific effects on ß3-adrenergic receptor cardiac regulation in two freshwater teleosts: channel catfish (ictalurus punctatus) and common carp (cyprinus carpio).β₃-adrenergic receptors (ar) are important in teleost cardiovascular regulation. to date, it is unknown whether temperature acclimation changes ß₃-ar functionality and consequently the involvement of this ar subtype in teleost cardiac regulation. common carp (cyprinus carpio) were acclimated at 12 °c or 23 °c (minimum 3 weeks) after which cardiovascular variables (cardiac output (q), stroke volume (sv) and heart rate (fh)) were measured upon injection of the ß₃-ar agonist, brl(37344), and antago ...201525882086
chronic exposure to tributyltin induces brain functional damage in juvenile common carp (cyprinus carpio).the aim of the present study was to investigate the effect of tributyltin (tbt) on brain function and neurotoxicity of freshwater teleost. the effects of long-term exposure to tbt on antioxidant related indices (mda, malondialdehyde; sod, superoxide dismutase; cat, catalase; gr, glutathione reductase; gpx, glutathione peroxidase), na+-k+-atpase and neurological parameters (ache, acetylcholinesterase; mao, monoamine oxidase; no, nitric oxide) in the brain of common carp were evaluated. fish were ...201525879203
evaluation of an extended-release formulation of ceftiofur crystalline-free acid in koi (cyprinus carpio).the use of an extended release ceftiofur crystalline-free acid formulation (ccfa, excede for swine(®) , pfizer animal health) in koi was evaluated after administration of single intramuscular (i.m.) or intracoelomic (i.c.) doses. twenty koi were divided randomly into a control group and four treatment groups (20 mg/kg i.m., 60 mg/kg i.m., 30 mg/kg i.c., and 60 mg/kg i.c.). serum ceftiofur-free acid equivalents (cfae) concentrations were quantified. the pharmacokinetic data were analyzed using a ...201525876672
transcriptome analysis of the plateau fish (triplophysa dalaica): implications for adaptation to hypoxia in fishes.triplophysa dalaica, endemic species of qinghai-tibetan plateau, is informative for understanding the genetic basis of adaptation to hypoxic conditions of high altitude habitats. here, a comprehensive gene repertoire for this plateau fish was generated using the illumina deep paired-end high-throughput sequencing technology. de novo assembly yielded 145, 256 unigenes with an average length of 1632 bp. blast searches against genbank non-redundant database annotated 74,594 (51.4%) unigenes encodin ...201525869933
alkylphenol ethoxylates and brominated flame retardants in water, fish (carp) and sediment samples from the vaal river, south africa.alkylphenol ethoxylates (apes) and brominated flame retardants (bfrs) are known to be bio-accumulative, persistent, and endocrine disruptors and can cause adverse health effects in animals and humans. in this study, environmental samples were collected from sites along the vaal river, south africa in order to determine the concentrations of apes and bfrs in water, sediment, and fish samples. the highest concentrations of these pollutants were observed from discharge of the rietspruit wwtw. measu ...201525869432
involvement of the mitfa gene in the development of pigment cell in japanese ornamental (koi) carp (cyprinus carpio l.).a colored phenotype is an important feature of ornamental fish. in mammals, microphthalmia-associated transcription factor (mitf) was found to regulate the development of melanocytes. in this study, the mitfa cdna was first cloned from the japanese ornamental (koi) carp (cyprinus carpio l.), an important ornamental freshwater fish. the full-length cdna of the mitfa gene contains 1634 bp, coding for 412 amino acids in koi. the identity degree of mitfa amino acid sequences between the koi carp and ...201525867426
adaptive expression of uncoupling protein 1 in the carp liver and kidney in response to changes in ambient temperature.the expression of uncoupling protein (ucp1) is up-regulated in mammalian brown adipocytes during cold exposure. however, a previous study revealed that ucp1 was highly expressed in the liver of common carps, and that the hepatic expression of ucp1 was down-regulated during cold exposure. the present study examined the effects of temperature on the recovery of ucp1 expression levels and the expression of genes involved in ucp1 transcription in the livers and kidneys of common carps. the hepatic a ...201525865906
benzo(a)pyrene-induced cytochrome p4501a expression of four freshwater fishes (oryzias latipes, danio rerio, cyprinus carpio, and zacco platypus).oryzias latipes, danio rerio, cyprinus carpio, and zacco platypus are useful indicator species for cyp1a biomarker studies; however, comparative studies have not been performed. to compare susceptibility, dose- and time-dependent cyp1a induction at the mrna and protein levels in response to benzo(a)pyrene (bap) exposure was analyzed. at the mrna level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. c. carpio showed th ...201525863331
description of myxobolus balatonicus n. sp. (myxozoa: myxobolidae) from the common carp cyprinus carpio l. in lake balaton.myxobolus balatonicus n. sp. was detected in the gill filaments of the common carp cyprinus carpio l. collected in lake balaton, hungary. its oval plasmodia measuring 600-800 × 300-400 µm were located intravasally in the filamental arteries. the spores measured 11.2 ± 0.92 × 9.5 ± 0.41 × 7.4 ± 0.33 µm and had two equal polar capsules with six filamental turns. both morphology and dna sequence analysis revealed that m. balatonicus n. sp. is distinct from the ten species of myxobolus bütschli, 188 ...201525862034
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