Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| an uncommon case of hepatopulmonary amoebiasis. | amoebiasis is an uncommon infection in developed countries caused by the protozoan entamoeba histolytica. amoebic liver abscess is the most frequent extraintestinal presentation of the disease; pleuropulmonary involvement is rare, occurring mostly by rupture of the abscess into the pleural space or lung parenchyma. we describe a case of a 48-year-old migrant from são tomé e príncipe, with fever, wasting, dry cough and right upper abdominal pain for the past 2 months. the ct scan revealed a volum ... | 2014 | 25155487 |
| flavodiiron oxygen reductase from entamoeba histolytica: modulation of substrate preference by tyrosine 271 and lysine 53. | flavodiiron proteins (fdps) are a family of enzymes endowed with bona fide oxygen- and/or nitric-oxide reductase activity, although their substrate specificity determinants remain elusive. after a comprehensive comparison of available three-dimensional structures, particularly of fdps with a clear preference toward either o2 or no, two main differences were identified near the diiron active site, which led to the construction of site-directed mutants of tyr(271) and lys(53) in the oxygen reducin ... | 2014 | 25151360 |
| in vitro antiamoebic activity evaluation and docking studies of metronidazole-triazole hybrids. | an in-house database of 520 compounds was docked against entamoeba histolytica thioredoxin reductase (ehtrr), a promising target for the treatment of amoebiasis. amongst these, some metronidazole (mtz)-triazole hybrids were ranked high, with docking scores from -10.23 to -7.56. studies of the binding orientations and conformations show that the head groups of mtz-triazole hybrids interact with the arginine residues within the binding pocket of ehtrr, making it clear that such is the optimal and ... | 2014 | 25146853 |
| genetic analysis of enterobius vermicularis isolated from a chimpanzee with lethal hemorrhagic colitis and pathology of the associated lesions. | human pinworms, enterobius vermicularis, are normally recognized as minor pathogens. however, a fatal case of human pinworm infection has been reported in a nonhuman primate, a zoo reared chimpanzee. here, we histopathologically examined the lesions in tissues from the deceased chimpanzee and genetically characterized the isolated worms to investigate the pathogenicity and determine the phylogeny. we identified ulcers deep in the submucosa where many parasites were found to have invaded the lami ... | 2014 | 25138069 |
| genetic variability of the serine-rich entamoeba histolytica protein gene in clinical isolates from the united arab emirates. | the genetic diversity of 20 entamoeba histolytica isolates from asymptomatic individuals from the uae was investigated by analyzing polymorphism in the serine-rich e. histolytica gene (srehp) by nested polymerase chain reaction (pcr) amplification followed by restriction fragment length polymorphism (rflp) on dna extracted directly from stool samples. the srehp gene was successfully amplified in 15 out of 20 e. histolytica-positive samples. four out of the remaining five isolates did not amplify ... | 2014 | 25134907 |
| proteomic analysis of entamoeba histolytica in vivo assembled pre-mrna splicing complexes. | the genome of the human intestinal parasite entamoeba histolytica contains nearly 3000 introns and bioinformatic predictions indicate that major and minor spliceosomes occur in entamoeba. however, except for the u2-, u4-, u5- and u6 snrnas, no other splicing factor has been cloned and characterized. here, we ha-tagged cloned the snrnp component u1a and assessed its expression and nuclear localization. because the snrnp-free u1a form interacts with polyadenylate-binding protein, ha-u1a immunoprec ... | 2014 | 25109466 |
| recent advances in molecular biology of parasitic viruses. | the numerous protozoa that can inhabit the human gastro-intestinal tract are known, yet little is understood of the viruses which infect these protozoa. the discovery, morphologic details, purification methods of virus-like particles, genome and proteome of the parasitic viruses, entamoeba histolytica, giardia lamblia, trichomonas vaginalis, and the eimeria sp. are described in this review. the protozoan viruses share many common features: most of them are rna or double-stranded rna viruses, ran ... | 2014 | 25019235 |
| entamoeba histolytica adaptation to glucose starvation: a matter of life and death. | parasites are often challenged by constant changes of the glucose concentration in their different hosts and/or within the different biotopes in the same host. during its life cycle, entamoeba histolytica, the causative protozoan parasite of human amoebiasis, is exposed to both a glucose-poor environment in the colon and a glucose-rich environment in the liver. high-throughput 'omics' technologies are now widely used to characterize the cell's global response to various stresses and these techno ... | 2014 | 25016247 |
| differential expression and immunolocalization of antioxidant enzymes in entamoeba histolytica isolates during metronidazole stress. | entamoeba histolytica infections are endemic in the indian subcontinent. five to eight percent of urban population residing under poor sanitary conditions suffers from entamoeba infections. metronidazole is the most widely prescribed drug used for amoebiasis. in order to understand the impact of metronidazole stress on the parasite, we evaluated the expression of two antioxidant enzymes, peroxiredoxin and fesod, in entamoeba histolytica isolates during metronidazole stress. the results reveal th ... | 2014 | 25013795 |
| crystallization and preliminary x-ray analysis of rabx3, a tandem gtpase from entamoeba histolytica. | ras superfamily gtpases regulate signalling pathways that control multiple biological processes by modulating the gtp/gdp cycle. various rab gtpases, which are the key regulators of vesicular trafficking pathways, play a vital role in the survival and virulence of the enteric parasite entamoeba histolytica. the rab gtpases act as binary molecular switches that utilize the conformational changes associated with the gtp/gdp cycle to elicit responses from target proteins and thereby regulate a broa ... | 2014 | 25005092 |
| structural elucidation of a dual-activity pap phosphatase-1 from entamoeba histolytica capable of hydrolysing both 3'-phosphoadenosine 5'-phosphate and inositol 1,4-bisphosphate. | the enzyme 3'-phosphoadenosine 5'-phosphatase-1 (pap phosphatase-1) is a member of the li(+)-sensitive mg(2+)-dependent phosphatase superfamily, or inositol monophosphatase (impase) superfamily, and is an important regulator of the sulfate-activation pathway in all living organisms. inhibition of this enzyme leads to accumulation of the toxic byproduct 3'-phosphoadenosine 5'-phosphate (pap), which could be lethal to the organism. genomic analysis of entamoeba histolytica suggests the presence of ... | 2014 | 25004978 |
| regulation of virulence of entamoeba histolytica. | entamoeba histolytica is the third-leading cause of parasitic mortality globally. e. histolytica infection generally does not cause symptoms, but the parasite has potent pathogenic potential. the origins, benefits, and triggers of amoebic virulence are complex. amoebic pathogenesis entails depletion of the host mucosal barrier, adherence to the colonic lumen, cytotoxicity, and invasion of the colonic epithelium. parasite damage results in colitis and, in some cases, disseminated disease. both ho ... | 2014 | 25002094 |
| proteomic analysis identifies endoribouclease ehl-psp and ehrrp41 exosome protein as novel interactors of ehcaf1 deadenylase. | in higher eukaryotic cells mrna degradation initiates by poly(a) tail shortening catalyzed by deadenylases caf1 and ccr4. in spite of the key role of mrna turnover in gene expression regulation, the underlying mechanisms remain poorly understood in parasites. here, we aimed to study the function of ehcaf1 and identify associated proteins in entamoeba histolytica. by biochemical assays, we evidenced that ehcaf1 has both rna binding and deadenylase activities in vitro. ehcaf1 was located in cytopl ... | 2014 | 24998979 |
| liver abscess in the tropics: an experience from jammu. | to study the various types of liver abscesses. this prospective study was conducted over a period of one year, from november 2011 to october 2012, at the department of general surgery in acharya shri chander college of medical sciences and hospital sidhra, jammu. | 2014 | 24996785 |
| balantidiosis: a rare accidental finding in the urine of a patient with acute renal failure. | balantidium coli is the only ciliated protozoan which is known to infect human and nonhuman primates. route of infection is faecal-oral route. it is actively motile and causes mostly asymptomatic infections, or it may develop dysentery which is similar to that which is caused by entamoeba histolytica. here, we are describing a case of an accidental finding of b.coli in the urine of a patient who presented with acute renal failure, based on its characteristic morphology and motility which were se ... | 2014 | 24995185 |
| modified pehps medium as an alternative for the in vitro culture of giardia lamblia. | commercial culture media present interlot variations in biological activity. we have previously designed a homemade and economic culture medium, pehps medium, for the axenic cultivation of entamoeba histolytica and trichomonas vaginalis. trophozoites of amoebae and trichomonads grow well in this medium. furthermore, the medium is stable for several months when stored frozen or refrigerated. the objective of this work was to modify pehps medium to support the in vitro growth of giardia lamblia. i ... | 2014 | 24982905 |
| antiprotozoal activity of 8-acyl and 8-alkyl incomptine a analogs. | the activities of 11 c-8-o-acyl and alkyl analogs of the antiprotozoal sesquiterpene lactone, incomptine a (1) against entamoeba histolytica and giardia lamblia, were determined. here, the effects of different lengths and amounts of branching of the acyl and alkyl groups on the antiprotozoal activity of the synthesized incomptine a-analogs are reported. | 2014 | 24974343 |
| in vitro genotoxic and mutagenic evaluation of the aqueous extract of codiaeum variegatum and its amoebicidal sub-fraction. | codiaeum variegatum, grown in many varieties, has been widely used as a houseplant based on its brightly decorative foliage. in addition, a variety of this plant has been used for a long time against bloody diarrhea by the local population in cameroon. | 2014 | 24971796 |
| analysis of the epithelial damage produced by entamoeba histolytica infection. | entamoeba histolytica is the causative agent of human amoebiasis, a major cause of diarrhea and hepatic abscess in tropical countries. infection is initiated by interaction of the pathogen with intestinal epithelial cells. this interaction leads to disruption of intercellular structures such as tight junctions (tj). tj ensure sealing of the epithelial layer to separate host tissue from gut lumen. recent studies provide evidence that disruption of tj by the parasitic protein ehcpadh112 is a prere ... | 2014 | 24962382 |
| ip6k structure and the molecular determinants of catalytic specificity in an inositol phosphate kinase family. | inositol trisphosphate kinases (ip3ks) and inositol hexakisphosphate kinases (ip6ks) each regulate specialized signalling activities by phosphorylating either insp3 or insp6 respectively. the molecular basis for these different kinase activities can be illuminated by a structural description of ip6k. here we describe the crystal structure of an entamoeba histolytica hybrid ip6k/ip3k, an enzymatic parallel to a 'living fossil'. through molecular modelling and mutagenesis, we extrapolated our find ... | 2014 | 24956979 |
| metabolomic analysis of entamoeba: applications and implications. | entamoeba histolytica is an enteric protozoan parasite that causes hemorrhagic dysentery and extraintestinal abscesses in millions of inhabitants of endemic areas. the genome of e. histolytica has already been sequenced and used to predict the metabolic potential of the organism. since nearly 56% of the e. histolytica genes remain unannotated, correlative 'omics' analyses of genomics, transcriptomics, proteomics, and biochemical metabolic profiling are essential in uncovering new, or poorly unde ... | 2014 | 24950028 |
| inferior vena cava thrombosis in a case of amoebic liver abscess: is hypercomplementemia responsible for this rare entity? | among the liver abscesses, thrombosis of the inferior vena cava (ivc) has been reported mainly in amoebic liver abscess (ala) caused by entamoeba histolytica (e.h). it is an unusual complication especially in paediatric age group. association of hypercomplementemia and ivc thrombosis has not been discussed previously. published data suggest that e.h can activate the complement system and can cause hypercomplementemia. a very few studies suggest that complement activation and hypercomplementemia ... | 2014 | 24943781 |
| destabilization domain approach adapted for regulated protein expression in the protozoan parasite entamoeba histolytica. | a plethora of information has been gained by sequencing the genome of the human parasite entamoeba histolytica, however a lack of robust genetic tools hampers experimental elucidation of gene functions. we adapted the destabilization domain approach for modulation of protein levels in e. histolytica using the destabilization domains of fk506 binding protein (ddfkbp) and dihydrofolate reductase (dddhfr), respectively. in our studies, the ddfkbp appears to be more tightly regulated than dddhfr, wi ... | 2014 | 24929134 |
| atomic resolution crystal structure of vclmwptp-1 from vibrio cholerae o395: insights into a novel mode of dimerization in the low molecular weight protein tyrosine phosphatase family. | low molecular weight protein tyrosine phosphatase (lmwptp) is a group of phosphotyrosine phosphatase ubiquitously found in a wide range of organisms ranging from bacteria to mammals. dimerization in the lmwptp family has been reported earlier which follows a common mechanism involving active site residues leading to an enzymatically inactive species. here we report a novel form of dimerization in a lmwptp from vibrio cholera 0395 (vclmwptp-1). studies in solution reveal the existence of the dime ... | 2014 | 24909685 |
| ligand heterogeneity of the cysteine protease binding protein family in the parasitic protist entamoeba histolytica. | lysosomal soluble proteins are targeted to endosomes and lysosomes by specific receptors resident in the endoplasmic reticulum and/or the golgi apparatus. the enteric protozoan parasite entamoeba histolytica has a novel class of lysosomal targeting receptors, named the cysteine protease binding protein family (cpbf). among 11 cpbfs (cpbf1-11), ligands for three members, cpbf1, cpbf6 and cpbf8, were previously shown to be cysteine proteases, α- and γ- amylases, and β-hexosaminidase and lysozymes, ... | 2014 | 24907554 |
| ultrastructural characteristics and molecular identification of entamoeba suis isolated from pigs with hemorrhagic colitis: implications for pathogenicity. | protozoan parasites of the genus entamoeba infect many classes of vertebrates and are primarily classified based on morphological criteria. to date, only a few species have been proven to cause disease. here, we examined the pathology of infected pigs with hemorrhage and detected entamoeba parasites. isolates were characterized genetically and ultrastructurally to identify the species. histopathologically, bleeding and thrombus formation were seen only in the large intestine mucosa, where a larg ... | 2014 | 24894081 |
| the flavonoid (-)-epicatechin affects cytoskeleton proteins and functions in entamoeba histolytica. | human amoebiasis is an intestinal disease with a global distribution. due to reports of parasite resistance or susceptibility reduction to metronidazole treatment, there is a renewed interest for the search of new molecules with antiamoebic activity. the flavonoid (-)-epicatechin that was isolated from the mexican medicinal plant geranium mexicanum hbk has an in vitro activity against e. histolytica trophozoites, however its molecular effects have been poorly documented. using a proteomic approa ... | 2014 | 24887480 |
| structural and mutational analysis of amino acid residues involved in atp specificity of escherichia coli acetate kinase. | acetate kinase (ak) generally utilizes atp as a phosphoryl donor, but ak from entamoeba histolytica (ppi-ehiak) uses pyrophosphate (ppi), not atp, and is ppi-specific. the determinants of the phosphoryl donor specificity are unknown. here, we inferred 5 candidate amino acid residues associated with this specificity, based on structural information. each candidate residue in escherichia coli atp-specific ak (atp-ecoak), which is unable to use ppi, was substituted with the respective ppi-ehiak ami ... | 2014 | 24856051 |
| epidemiological features of intestinal infection with entamoeba histolytica in taiwan, 2002-2010. | amebiasis remains an important public health problem worldwide, and immigration and an increase in international travel have affected the incident cases of the disease. the purpose of this study was to assess the prevalence of entamoeba histolytica in taiwan between 2002 and 2010. we analyzed data from surveillance programs run by the centers for disease control, taiwan (taiwan cdc), and only laboratory-confirmed cases were analyzed. in total, 1796 cases with e. histolytica infections were inclu ... | 2014 | 24837854 |
| peroxynitrite and peroxiredoxin in the pathogenesis of experimental amebic liver abscess. | the molecular mechanisms by which entamoeba histolytica causes amebic liver abscess (ala) are still not fully understood. amebic mechanisms of adherence and cytotoxic activity are pivotal for amebic survival but apparently do not directly cause liver abscess. abundant evidence indicates that chronic inflammation (resulting from an inadequate immune response) is probably the main cause of ala. reports referring to inflammatory mechanisms of liver damage mention a repertoire of toxic molecules by ... | 2014 | 24822193 |
| hsp90 inhibitors as new leads to target parasitic diarrheal diseases. | entamoeba histolytica and giardia lamblia are anaerobic protozoan parasites that cause amebiasis and giardiasis, two of the most common diarrheal diseases worldwide. current therapy relies on metronidazole, but resistance has been reported and the drug has significant adverse effects. therefore, it is critical to search for effective, better-tolerated antiamebic and antigiardial drugs. we synthesized several examples of a recently reported class of hsp90 inhibitors and evaluated these compounds ... | 2014 | 24820073 |
| the cell surface proteome of entamoeba histolytica. | surface molecules are of major importance for host-parasite interactions. during entamoeba histolytica infections, these interactions are predicted to be of prime importance for tissue invasion, induction of colitis and liver abscess formation. to date, however, little is known about the molecules involved in these processes, with only about 20 proteins or protein families found exposed on the e. histolytica surface. we have therefore analyzed the complete surface proteome of e. histolytica. usi ... | 2014 | 24136294 |
| leptin signaling protects the gut from entamoeba histolytica infection. | the role of the adipose-derived hormone leptin, and leptin receptors, in signaling satiety to the central nervous system and regulating energy balance is well recognized. but leptin also acts on peripheral tissues such as skeletal muscles, adipose tissues, pancreas, liver, intestine and the immune system. the existence of different splice variants of leptin receptor and the numerous intracellular signaling pathways triggered by leptin make this a truly versatile system. two recent studies explor ... | 2014 | 22356851 |
| amoebic cervicitis mimicking posterior wall fibroid: a rare presentation. | amoebiasis of the uterine cervix is an extremely rare entity, and presentation as fibroid uterus has not been reported, to the best of our knowledge in our extensive search of the english literature. it can clinically simulate cervical malignancy by virtue of surface papillomatous and overall ulcerated and necrotic appearance. we present a case of amoebic infection of the cervix in a 45-year-old female which was suspected to be a posterior wall fibroid with degeneration until a histopathological ... | 2014 | 22743593 |
| [clinical case of the month. amoebic liver abscess contracted in india with diagnosis confirmed by pcr]. | amoebiasis is a disease of parasitic origin responsible for dysentery and extra-intestinal complications. the infection is by entamoeba histolytica, an amoeba whose geographical distribution is cosmopolitan, but that is more prevalent in tropical areas. only a few infections are symptomatic and some of them may cause extra-intestinal complications. hepatic amoebiasis is the most frequently observed. we report the case of a belgian woman who developed an amoebic liver abscess after returning from ... | 2015 | 24053104 |
| an amebic anti-inflammatory peptide down-regulates ex vivo il-1β expression in patients with rheumatoid arthritis. | the monocyte locomotion inhibitory factor (mlif) is a heat-stable pentapeptide produced by entamoeba histolytica in culture. this factor displays several anti-inflammatory properties (i.e., inhibition of locomotion and respiratory burst in monocytes, reduction of skin hypersensitivity and delay of mononuclear cells in human rebuck skin windows) with inhibition of adhesion molecules, chemokines, and other genes including interleukin-1β (il-1β). in animal models, it reduces carragenin-induced infl ... | 2015 | 22749729 |
| vagotomy induces deregulation of the inflammatory response during the development of amoebic liver abscess in hamsters. | the parasympathetic nervous system modulates the immune response in the abdominal-pelvic gut through the vagus nerve, which releases acetylcholine. this endogenous ligand acts on α7 nicotinic receptors expressed on immune cells. | 2015 | 24819982 |
| cellular and proteomics analysis of the endomembrane system from the unicellular entamoeba histolytica. | entamoeba histolytica is the protozoan parasite agent of amoebiasis, an infectious disease of the human intestine and liver. specific active pathogenic factors are secreted toward the external milieu upon interaction of the parasite with human tissue. trafficking dynamics and secretion of these factors is not known and characterization of the dynamics interplay of subcellular compartments such as the er or golgi apparatus is still pending. in this work, we took advantage of cell fractionation an ... | 2015 | 25109464 |
| prevalence of entamoeba histolytica -like cysts compared to e. histolytica antigens detected by elisa in the stools of 600 patients from three socioeconomic communities in the metropolitan city of lahore, pakistan. | amoebiasis, caused by entamoeba histolytica , has a worldwide distribution and is of public health significance in many developing countries. it has a fecal-oral transmission cycle and is most prevalent in developing countries in regions where substandard sanitary conditions exist due to poverty. little is known about the epidemiology of e. histolytica infection and its presence in different socioeconomic communities in developing countries. we undertook the present study in the city of lahore, ... | 2015 | 25189631 |
| headache following occipital brain lesion: a case of migraine triggered by occipital spikes? | this study describes the case of an 8-year-old boy who developed a genuine migraine after the surgical excision, from the right occipital lobe, of brain abscesses due to selective infestation of the cerebrum by entamoeba histolytica. after the surgical treatment, the boy presented daily headaches with typical migraine features, including right-side parieto-temporal pain, nausea, vomiting, and photophobia. electroencephalography (eeg) showed epileptiform discharges in the right occipital lobe, al ... | 2015 | 25406125 |
| in vivo identification of the steps that control energy metabolism and survival of entamoeba histolytica. | the steps that control the entamoeba histolytica glycolytic flux were here identified by elasticity analysis, an experimental approach of metabolic control analysis. the concentrations of glycolytic metabolites were gradually varied in live trophozoites by (a) feeding with different glucose concentrations and (b) inhibiting the final pathway steps; in parallel, the changes in the pathway flux were determined. from the metabolite concentration-flux relationship, the elasticity coefficients of ind ... | 2015 | 25350227 |
| colonoscopic diagnosis of amebiasis: a case series and systematic review. | nonspecific gastrointestinal symptoms make diagnosis of amebiasis difficult. certain colonoscopic findings predict amebic colitis while others suggest different diagnoses. we aimed to evaluate the diagnostic capability of colonic evaluation of amebiasis. | 2015 | 25346004 |
| [ameboma: possible therapeutic decisions in an amebiasis-endemic region]. | amebiasis can mimic cecal tumors. unless this infection is diagnosed in a timely manner, affected individuals may undergo extensive surgery. | 2015 | 25195079 |
| multisite clinical evaluation of a rapid test for entamoeba histolytica in stool. | rapid point-of-care detection of enteric protozoa in diarrheal stool is desirable in clinical and research settings to efficiently determine the etiology of diarrhea. we analyzed the ability of the third-generation e. histolytica quik chek assay developed by techlab to detect amebic antigens in fecal samples collected from independent study populations in south africa and bangladesh. we compared the performance of this recently released rapid test to that of the commercially available prospect e ... | 2015 | 25428152 |
| immunization with the entamoeba histolytica surface metalloprotease ehmsp-1 protects hamsters from amebic liver abscess. | diarrhea and amebic liver abscesses due to invasive entamoeba histolytica infections are an important cause of morbidity and mortality in the developing world. entamoeba histolytica adherence and cell migration, two phenotypes linked to virulence, are both aberrant in trophozoites deficient in the metallosurface protease ehmsp-1, which is a homologue of the leishmania vaccine candidate leishmanolysin (gp63). we examined the potential of ehmsp-1 for use as a vaccine antigen to protect against ame ... | 2015 | 25452550 |
| ascaris spp. and capillaria caudinflata infections in captive-bred crested ibis (nipponia nippon) in china. | crested ibis (nipponia nippon), an endan gered native bird, was called the "precious stone" of oriental birds. n. nippon was considered a critically endangered species in the iucn red list of threatened species and a first-class national protected animal in china. the chinese government had exerted considerable effort to protect the n. nippon population. an effective approach to increase the number of these birds was captive breeding. however, several pathogens, including parasites, could jeopar ... | 2015 | 25486916 |
| pulmonary thromboembolism complicating amebic liver abscess: first reported case in the united states-case report and literature review. | even in the absence of amebic colitis, amebic liver abscess (ala) is the most common extraintestinal complication of entamoeba histolytica infection. in the usa, it is most prevalent in middle aged immigrant males from endemic countries such as africa, mexico, and india. one of the complications of ala is inferior vena cava (ivc) thrombosis, which is believed to result from the mechanical compression of the ivc and the consequent thrombogenic nidus elicited from the resultant inflammatory respon ... | 2015 | 26294987 |
| synthesis and characterization with antineoplastic, biochemical, cytotoxic, and antimicrobial studies of schiff base cu(ii) ion complexes. | copper(ii) complexes containing two schiff base ligands derived from 2-hydroxybenzaldehyde with 2-aminophenol and 3-aminophenol have been synthesized and characterized by means of analytical, magnetic, and spectroscopic methods. bacteria, fungus, entamoeba histolytica, and antineoplastic activities of the synthesized complexes have been determined by monitoring the parameters cell growth inhibition, survival time of tumour mice, time-body relation, causing of intraperitoneal cells and macrophage ... | 2015 | 26294901 |
| modulation of endogenous cysteine protease inhibitor (icp) 1 expression in entamoeba histolytica affects amoebic adhesion to extracellular matrix proteins. | entamoeba histolytica is an enteric tissue-invading protozoan parasite that causes amoebic colitis and occasionally liver abscess in humans. during tissue invasion, amoebic adhesion to host components is an important event for host cell death leading to successful invasion and infection. among amoebic virulence factors, gal/galnac lectin is known to be major adhesion factor to host cells. in this study, we investigated the role of amoebic secreted cp (cysteine proteases) in amoebic adhesion to e ... | 2015 | 25500214 |
| a new anthraquinone from morinda elliptica ridl. | a new anthraquinone, morinquinone, together with 18 known anthraquinones were isolated from the stems of morinda elliptica ridl. their structures were elucidated on the basis of spectroscopic data. they each showed weak inhibitory activity against a susceptible strain of staphylococcus aureus and a methicillin-resistant s. aureus. damnacanthal was effective against microsporum gypseum (mic 1 μg/ml). lucidin was active against entamoeba histolytica (mic 31.25 μg/ml) and giardia intestinalis (mic ... | 2015 | 25686628 |
| molecular and functional characterisation of a stress responsive cysteine protease, ehcp6 from entamoeba histolytica. | entamoeba histolytica cysteine protease 6 (ehcp6) is a stress responsive cysteine protease that is upregulated in response to heat shock and during pathogen invasion of the host tissue. in the present study an attempt has been made to express and purify recombinant ehcp6 in order to gain insights into its biochemical properties. the recombinant and refolded protein has been shown to undergo autoproteolysis in the presence of dtt and sds to give rise to ∼25kda mature form. the mature form of the ... | 2015 | 25680771 |
| a pcr-rflp method for the simultaneous differentiation of three entamoeba species. | amoebiasis caused by entamoeba histolytica continues to be one of the most common parasitic diseases in the developing world. despite its relevance, due to the lack of accurate diagnostic methods, the true clinical and public health importance of this parasite remains uncertain. the aim of this study was to develop a new diagnostic tool to differentiate e.histolytica from the morphologically undistinguishable e.dispar and e.moshkovskii. we developed a specific, fast and simple pcr-rflp method th ... | 2015 | 25680279 |
| importance of microscopic stool examination in patients with diarrhoea. | loose motion is a common symptom in patients reporting to our hospital. as it is a small set up where only facility for microscopic stool examination is available, we designed this study to know how much microscopic stool examination can help us in management of patients with diarrhoea. | 2015 | 25672169 |
| entamoeba histolytica: adhesins and lectins in the trophozoite surface. | entamoeba histolytica is the causative agent of amebiasis in humans and is responsible for 100,000 deaths annually, making it the third leading cause of death due to a protozoan parasite. pathogenesis appears to result from the potent cytotoxic activity of the parasite, which kills host cells within minutes. although the mechanism is unknown, it is well established to be contact-dependent. the life cycle of the parasite alternates with two forms: the resistant cyst and the invasive trophozoite. ... | 2015 | 25671365 |
| comparison of four rapid diagnostic tests, elisa, microscopy and pcr for the detection of giardia lamblia, cryptosporidium spp. and entamoeba histolytica in feces. | microscopy is the diagnostic reference standard for the detection of parasites, but it is labor-intensive and requires experience. rapid diagnostic tests (rdts) can provide an alternative to microscopy. | 2015 | 25615719 |
| maintenance of intracellular hypoxia and adequate heat shock response are essential requirements for pathogenicity and virulence of entamoeba histolytica. | adhesion to cells, cytotoxicity and proteolysis are functions required for virulence and pathogenicity of entamoeba histolytica. however, there was no correlation between these in vitro functions and the early elimination of non-pathogenic e. dispar and non-virulent e. histolytica (nveh) in experimental amoebic liver abscesses developed in hamsters. thus, additional functions may be involved in amoebic pathogenicity and virulence. in the present study, an integral experimental assessment, includ ... | 2015 | 25611463 |
| is the evaluation of entamoeba histolytica infection in hiv-positive patients of any clinical significance? | amoebiasis caused by entamoeba histolytica (e. histolytica) is one of the most problematic parasitic infections worldwide. data regarding the effect of hiv-induced immunodeficiency on the status of e. histolytica infection are sparse in iran. this study aimed to assess the seroprevalence of anti-e. histolytica igg among iranian hiv patients. further, it determined whether the advancement of immunodeficiency accompanies an increased risk of amoebiasis. a total of 91 hiv-infected patients and 91 c ... | 2015 | 25597605 |
| prevalence of hiv infection and risky sexual practices among adult males with travel-associated entamoeba histolytica infection in taiwan, 2006-2013. | 2015 | 25593040 | |
| notes from the field: outbreak of diarrheal illness caused by shigella flexneri - american samoa, may-june 2014. | on may 9, 2014, a physician at hospital a in american samoa noticed an abnormally high number of children presenting to the emergency department with bloody diarrhea. based on preliminary testing of stool specimens, entamoeba histolytica infection was suspected as a possible cause. shigella was also suspected in a subset of samples. on may 22, the american samoa department of health requested assistance from cdc with the outbreak investigation. the goals of the investigation were to establish th ... | 2015 | 25590684 |
| copro prevalence and estimated risk of entamoeba histolytica in diarrheic patients at beni-suef, egypt. | amoebiasis diagnosis is usually based on microscopy that cannot differentiate pathogenic e. histolytica from morphologically identical non-pathogenic species. 194 fecal samples were collected from diarrheic &/or dysenteric patients and examined for entamoeba complex microscopically, e. histolytica/e. dispar coproantigen using ict and e. histolytica coproantigen using tech lab e. histolytica ii elisa test. entamoeba complex trophozoites/cysts, e. histolytica/e. dispar coproantigen and e. histolyt ... | 2015 | 25542044 |
| entamoeba histolytica racc selectively engages p21-activated kinase effectors. | rho family gtpases modulate actin cytoskeleton dynamics by signaling through multiple effectors, including the p21-activated kinases (paks). the intestinal parasite entamoeba histolytica expresses ∼20 rho family gtpases and seven isoforms of pak, two of which have been implicated in pathogenesis-related processes such as amoebic motility and invasion and host cell phagocytosis. here, we describe two previously unstudied pak isoforms, ehpak4 and ehpak5, as highly specific effectors of ehracc. a s ... | 2015 | 25529118 |
| ethanol and isopropanol in concentrations present in hand sanitizers sharply reduce excystation of giardia and entamoeba and eliminate oral infectivity of giardia cysts in gerbils. | enteric protozoan parasites, which are spread by the fecal-oral route, are important causes of diarrhea (giardia duodenalis) and amebic dysentery (entamoeba histolytica). cyst walls of giardia and entamoeba have a single layer composed of fibrils of β-1,3-linked galnac and β-1,4-linked glcnac (chitin), respectively. the goal here was to determine whether hand sanitizers that contain ethanol or isopropanol as the active microbicide might reduce transmission of these parasites. we found that treat ... | 2015 | 26282413 |
| taking a bite: amoebic trogocytosis in entamoeba histolytica and beyond. | entamoeba histolytica is a diarrheal pathogen with the ability to cause profound host tissue damage. this organism possesses contact-dependent cell killing activity, which is likely to be a major contributor to tissue damage. e. histolytica trophozoites were recently shown to ingest fragments of living human cells. it was demonstrated that this process, termed amoebic trogocytosis, contributes to cell killing. recent advances in ex vivo and 3-d cell culture approaches have shed light on mechanis ... | 2015 | 26277085 |
| multiple amoebic liver abscess as initial manifestation in hiv sero-positive male. | amoebic liver abscess is the most frequent extra-intestinal manifestation of entamoeba histolytica infection. immunosuppression is known to predispose to amoebic liver abscess. although amoebic liver abscess is seen more commonly in patients of human-immunodeficiency virus (hiv), first presentation of hiv sero-positive patient as multiple liver abscess is quite uncommon.the author reports an unusual case of multiple liver abscesses in an hiv seropositive patient. this middle aged male with histo ... | 2015 | 26266151 |
| magnetic removal of entamoeba cysts from water using chitosan oligosaccharide-coated iron oxide nanoparticles. | amebiasis, a major health problem in developing countries, is the second most common cause of death due to parasitic infection. amebiasis is usually transmitted by the ingestion of entamoeba histolytica cysts through oral-fecal route. herein, we report on the use of chitosan oligosaccharide-functionalized iron oxide nanoparticles for efficient capture and removal of pathogenic protozoan cysts under the influence of an external magnetic field. these nanoparticles were synthesized through a chemic ... | 2015 | 26261417 |
| the effect of ifn-γ and tgf-β in the functional activity of mononuclear cells in the presence of entamoeba histolytica. | entamoeba histolytica (e. histolytica) causes amoebiasis, which is a disease with significant morbidity and mortality. phagocytic cells and cytokines appear to be important in amoebiasis, but very little is known about the influence of these cells and cytokines in protozoan infections. the aim of this study was to analyse the supernatant of cultures of mononuclear (mn) cells with e. histolytica to determine: 1) the levels of the cytokines ifn-γ and tgf-β, and 2) the amoebicidal activity of mn ce ... | 2015 | 26249205 |
| high throughput sequencing of entamoeba 27nt small rna population reveals role in permanent gene silencing but no effect on regulating gene expression changes during stage conversion, oxidative, or heat shock stress. | the human parasite entamoeba histolytica has an active rna interference (rnai) pathway with an extensive repertoire of 27nt small rnas that silence genes. however the role of this pathway in regulating amebic biology remains unknown. in this study, we address whether silencing via 27nt small rnas may be a mechanism for controlling gene expression changes during conversion between the trophozoite and cyst stages of the parasite. we sequenced small rna libraries generated from trophozoites, early ... | 2015 | 26248204 |
| post-transcriptional regulation of ribosomal protein genes during serum starvation in entamoeba histolytica. | ribosome synthesis involves all three rna polymerases which are co-ordinately regulated to produce equimolar amounts of rrnas and ribosomal proteins (rps). unlike model organisms where transcription of rrna and rp genes slows down during stress, in e. histolytica rdna transcription continues but pre-rrna processing slows down and unprocessed pre-rrna accumulates during serum starvation. to investigate the regulation of rp genes under stress we measured transcription of six selected rp genes from ... | 2015 | 26247142 |
| ehvps32 is a vacuole-associated protein involved in pinocytosis and phagocytosis of entamoeaba histolytica. | here, we investigated the role of ehvps32 protein (a member of the endosomal-sorting complex required for transport) in endocytosis of entamoeba histolytica, a professional phagocyte. confocal microscopy, tem and cell fractionation revealed ehvps32 in cytoplasmic vesicles and also located adjacent to the plasma membrane. between 5 to 30 min of phagocytosis, ehvps32 was detected on some erythrocytes-containing phagosomes of acidic nature, and at 60 min it returned to cytoplasmic vesicles and also ... | 2015 | 26230715 |
| a single rnaseiii domain protein from entamoeba histolytica has dsrna cleavage activity and can help mediate rnai gene silencing in a heterologous system. | dicer enzymes process double-stranded rna (dsrna) into small rnas that target gene silencing through the rna interference (rnai) pathway. dicer enzymes are complex, multi-domain rnaseiii proteins, however structural minimalism of this protein has recently emerged in parasitic and fungal systems. the most minimal dicer, saccharomyces castellii dicer1, has a single rnaseiii domain and two double stranded rna binding domains. in the protozoan parasite entamoeba histolytica 27nt small rnas are abund ... | 2015 | 26230096 |
| kaempferol inhibits entamoeba histolytica growth by altering cytoskeletal functions. | the flavonoid kaempferol obtained from helianthemum glomeratum, an endemic mexican medicinal herb used to treat gastrointestinal disorders, has been shown to inhibit growth of entamoeba histolytica trophozoites in vitro; however, the mechanisms associated with this activity have not been documented. several works reported that kaempferol affects cytoskeleton in mammalian cells. in order to gain insights into the action mechanisms involved in the anti-amoebic effect of kaempferol, here we evaluat ... | 2015 | 26620675 |
| enhancing protein capture using a combination of nanoyeast single-chain fragment affinity reagents and alternating current electrohydrodynamic forces. | new high-performance detection technologies and more robust protein capture agents can be combined to both rapidly and specifically capture and detect protein biomarkers associated with disease in complex biological samples. here we demonstrate the use of recently developed recombinant affinity reagents, namely nanoyeast-scfv, in combination with alternating current electrohydrodynamic (ac-ehd)-induced shear forces, to enhance capture performance during protein biomarker analysis. the use of ac- ... | 2015 | 26551436 |
| intestinal parasitic infections among children under five years of age presenting with diarrhoeal diseases to two public health facilities in hawassa, south ethiopia. | diarrhoea is the leading cause of morbidity and mortality in children under 5 years of age in developing countries, including ethiopia. it is caused by a wide range of pathogens, including parasites, bacteria and viruses. the aim of this study was to determine the prevalence of infection with intestinal parasites (ips) (and types) among children under 5 years of age with diarrhoeal diseases. | 2015 | 26530964 |
| heat shock protein 90 regulates encystation in entamoeba. | enteric protozoan entamoeba histolytica is a major cause of debilitating diarrheal infection worldwide with high morbidity and mortality. even though the clinical burden of this parasite is very high, this infection is categorized as a neglected disease. parasite is transmitted through feco-oral route and exhibit two distinct stages namely - trophozoites and cysts. mechanism and regulation of encystation is not clearly understood. previous studies have established the role of heat shock protein ... | 2015 | 26528271 |
| methodology for preservation of yeast-bound single chain fragment variable antibody affinity reagents. | readily accessible affinity reagents are critical to the validation of biomarkers and to the development of new diagnostic tests. as alternatives to monoclonal antibodies, yeast-bound single chain fragment variable antibody (yeast-scfv) can be rapidly selected from yeast display libraries. an important characteristic for any diagnostic reagent is its stability or ability to store it. a lyophilization procedure that has extended the shelf life of yeast-scfv by a factor of ≥10-fold relative to pre ... | 2015 | 26571425 |
| fatal case of amoebic liver abscess in a child. | we reported a case of amoebic liver abscess (ala) in a 6-year-old malaysian boy who presented with fever, lethargy, diarrhoea and right hypochondriac pain. on admission he was diagnosed with perforated acute appendicitis and a laparotomy was done. after surgery he developed acute respiratory distress. ultrasonography, chest x-ray and ct scan revealed two alas in the posterior segment of right lobe of liver, pleural effusion and collapsed consolidation of lungs bilaterally. percutaneous liver abs ... | 2015 | 26522307 |
| the first case of huge amebic intra-abdominal tumor with asymptomatic amebic colitis. | we report a rare case of huge amebic intra-abdominal tumor with asymptomatic amebic colitis. this appears to represent the first report of amebic intra-abdominal tumor. a 31-year-old woman presented to a local doctor with only a sensation of abdominal fullness. abdominal computed tomography (ct) showed a huge intra-abdominal tumor in the left abdominal cavity, and she was referred to our hospital. colonofiberscopy for detailed examination showed multiple slight, discrete ulcers in the cecum. ame ... | 2015 | 26366345 |
| novel hemagglutinating, hemolytic and cytotoxic activities of the intermediate subunit of entamoeba histolytica lectin. | galactose and n-acetyl-d-galactosamine (gal/galnac) inhibitable lectin of entamoeba histolytica, a common protozoan parasite, has roles in pathogenicity and induction of protective immunity in mouse models of amoebiasis. the lectin consists of heavy (hgl), light (lgl), and intermediate (igl) subunits. hgl has lectin activity and lgl does not, but little is known about the activity of igl. in this study, we assessed various regions of igl for hemagglutinating activity using recombinant proteins e ... | 2015 | 26354528 |
| a whole-genome rnai screen uncovers a novel role for human potassium channels in cell killing by the parasite entamoeba histolytica. | the parasite entamoeba histolytica kills human cells resulting in ulceration, inflammation and invasion of the colonic epithelium. we used the cytotoxic properties of ameba to select a genome-wide rnai library to reveal novel host factors that control susceptibility to amebic killing. we identified 281 candidate susceptibility genes and bioinformatics analyses revealed that ion transporters were significantly enriched among susceptibility genes. potassium (k(+)) channels were the most common tra ... | 2015 | 26346926 |
| a surface membrane protein of entamoeba histolytica functions as a receptor for human chemokine il-8: its role in the attraction of trophozoites to inflammation sites. | entamoeba histolytica trophozoites respond to the presence of il-8, moving by chemotaxis towards the source of the chemokine. il-8 binds to the trophozoite membrane and triggers a response that activates signaling pathways that in turn regulate actin/myosin cytoskeleton organisation to initiate migration towards the chemokine, suggesting the presence of a receptor for il-8 in the parasite. antibodies directed to the human il-8 receptor (cxcr1) specifically recognised a 29 kda protein in trophozo ... | 2015 | 26343219 |
| entamoeba invadens: identification of a serca protein and effect of serca inhibitors on encystation. | calcium has an important role on signaling of different cellular processes, including growth and differentiation. signaling by calcium also has an essential function in pathogenesis and differentiation of the protozoan parasites entamoeba histolytica and entamoeba invadens. however, the proteins of these parasites that regulate the cytoplasmic concentration of this ion are poorly studied. in eukaryotic cells, the calcium-atpase of the serca type plays an important role in calcium homeostasis by ... | 2015 | 26318877 |
| serosurvey of entamoeba histolytica exposure among tepehuanos population in durango, mexico. | the seroepidemiology of entamoeba histolytica infection in tepehuanos population in mexico is largely unknown. this study aimed to study the seroprevalence and correlates of e. histolytica antibodies in tepehuanos in durango, mexico. through a cross-sectional study, we determined the frequency of e. histolytica igg antibodies in 156 tepehuanos people in durango, mexico using an enzyme-linked immunoassay. furthermore, we studied the association of e. histolytica seroprevalence with the socio-demo ... | 2015 | 26199578 |
| antiamoebic and antigiardial activity of clerodane diterpenes from mexican salvia species used for the treatment of diarrhea. | terpenoids from salvia species have been identified to possess biological properties as antiprotozoal agents. here, we evaluated the antiamoebic and antigiardial activities of 14 known clerodane and modified clerodane-type diterpenes isolated from five mexican salvia species against entamoeba histolytica and giardia lamblia, and analyzed the effects of the functionalities in decalin ring or in the whole clerodane framework to visualize the structural requirements necessary to produce an antiprot ... | 2015 | 26190323 |
| metronidazole hydrazone conjugates: design, synthesis, antiamoebic and molecular docking studies. | metronidazole hydrazone conjugates (2-13) were synthesized and screened in vitro for antiamoebic activity against hm1: imss strain of entamoeba histolytica. six compounds were found to be better inhibitors of e. histolytica than the reference drug metronidazole. these compounds showed greater than 50-60% viability against hela cervical cancer cell line after 72 h treatment. also, molecular docking study was undertaken on e. histolytica thioredoxin reductase (ehthrase) protein which showed signif ... | 2015 | 26174554 |
| molecular biology research to benefit patients with entamoeba histolytica infection. | the development of molecular microbiology has made it possible for us to deepen our understanding of the pathogenesis of amebiasis. research using the trophozoite form of entamoeba histolytica has clearly shown us the importance of the interface between the parasite and host cells in vitro. immuno-pathogenesis after excystation was similarly well advanced by the use of a novel murine model of amebic colitis. however, it is still challenging to apply these findings to clinical and epidemiological ... | 2015 | 26173474 |
| dimethylated h3k27 is a repressive epigenetic histone mark in the protist entamoeba histolytica and is significantly enriched in genes silenced via the rnai pathway. | rna interference (rnai) is a fundamental biological process that plays a crucial role in regulation of gene expression in many organisms. transcriptional gene silencing (tgs) is one of the important nuclear roles of rnai. our previous data show that entamoeba histolytica has a robust rnai pathway that links to tgs via argonaute 2-2 (ago2-2) associated 27-nucleotide small rnas with 5'-polyphosphate termini. here, we report the first repressive histone mark to be identified in e. histolytica, dime ... | 2015 | 26149683 |
| in entamoeba histolytica, a bspa family protein is required for chemotaxis toward tumour necrosis factor. | entamoeba histolytica cell migration is essential for the development of human amoebiasis (an infectious disease characterized by tissue invasion and destruction). the tissue inflammation associated with tumour necrosis factor (tnf) secretion by host cells is a well-documented feature of amoebiasis. tumour necrosis factor is a chemoattractant for e. histolytica, and the parasite may have a tnf receptor at its cell surface. | 2015 | 28357299 |
| entamoeba histolytica - tumor necrosis factor: a fatal attraction. | 2015 | 28357296 | |
| magnetic resonance imaging of pathogenic protozoan parasite entamoeba histolytica labeled with superparamagnetic iron oxide nanoparticles. | the aim of this study was to establish a noninvasive tracking of the pathogenic parasite entamoeba histolytica (eh) after superparamagnetic iron oxide (spio) labeling by magnetic resonance imaging (mri) on a single-cell level in vitro and in vivo in a mouse model for amebic liver abscess (ala). | 2015 | 26135016 |
| detection of entamoeba histolytica by recombinase polymerase amplification. | amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. traditional microscopy approaches are neither sensitive nor specific for entamoeba histolytica. antigen assays are more specific, but many cases are missed unless tested by molecular methods. although polymerase chain reaction (pcr) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource- ... | 2015 | 26123960 |
| [drainage of amoebic liver abscess by single incision laparoscopic surgery. report of a case]. | single incision laparoscopic surgery has increased recently due to successful results, achieved in several procedures. the aim of the present work is to present the first case in which single incision laparoscopy is used for the drainage of an amoebic liver abscess. | 2015 | 26118779 |
| molecular detection of the carriage rate of four intestinal protozoa with real-time polymerase chain reaction: possible overdiagnosis of entamoeba histolytica in nigeria. | diarrhea remains the second largest killer of children worldwide, and nigeria ranks number two on the list of global deaths attributable to diarrhea. meanwhile, prevalence studies on potentially diarrheagenic protozoa in asymptomatic carriers using molecular detection methods remain scarce in sub-saharan countries. to overcome sensitivity issues related to microscopic detection and identification of cysts in stool concentrates, real-time polymerase chain reaction (pcr) was used to analyze genomi ... | 2015 | 26101274 |
| insights into endosomal maturation of human holo-transferrin in the enteric parasite entamoeba histolytica: essential roles of rab7a and rab5 in biogenesis of giant early endocytic vacuoles. | the pathogenic amoeba entamoeba histolytica is one of the causative agents of health hazards in tropical countries. it causes amoebic dysentery, colitis and liver abscesses in human. iron is one of the essential nutritional resources for survival and chronic infection caused by the amoeba. the parasite has developed multiple ways to import, sequester and utilize iron from various iron-binding proteins from its host. in spite of its central role in pathogenesis, the mechanism of iron uptake by th ... | 2015 | 26096601 |
| protection against amoebic liver abscess in hamster by intramuscular immunization with an autographa californica baculovirus driving the expression of the gal-lectin lc3 fragment. | in a previous study, we demonstrated that oral immunization using autographa californica baculovirus driving the expression of the gal-lectin lc3 fragment (acnpv-lc3) of entamoeba histolytica conferred protection against ala development in hamsters. in this study, we determined the ability of acnpv-lc3 to protect against ala by the intramuscular route as well as the liver immune response associated with protection. results showed that 55% of hamsters im immunized with acnpv-lc3 showed sterile pr ... | 2015 | 26090442 |
| binding and endocytosis of bovine hololactoferrin by the parasite entamoeba histolytica. | entamoeba histolytica is a human parasite that requires iron (fe) for its metabolic function and virulence. bovine lactoferrin (b-lf) and its peptides can be found in the digestive tract after dairy products are ingested. the aim of this study was to compare virulent trophozoites recently isolated from hamster liver abscesses with nonvirulent trophozoites maintained for more than 30 years in cultures in vitro regarding their interaction with iron-charged b-lf (b-holo-lf). we performed growth kin ... | 2015 | 26090404 |
| cytotoxicity of protein-carbon nanotubes on j774 macrophages is a functionalization grade-dependent effect. | carbon nanotubes (cnts) are used as carriers in medicine due to their ability to be functionalized with chemical substances. however, cytotoxicity analysis is required prior to use for in vivo models. the aim of this study was to evaluate the cytotoxic effect of cnts functionalized with a 46 kda surface protein from entamoeba histolytica (p46-cnts) on j774a macrophages. with this purpose, cnts were synthesized by spray pyrolysis and purified (p-cnts) using sonication for 48 h. a 46 kda protein, ... | 2015 | 26075262 |
| chew on this: amoebic trogocytosis and host cell killing by entamoeba histolytica. | entamoeba histolytica was named 'histolytica' (from histo-, 'tissue'; lytic-, 'dissolving') for its ability to destroy host tissues. direct killing of host cells by the amoebae is likely to be the driving factor that underlies tissue destruction, but the mechanism was unclear. we recently showed that, after attaching to host cells, amoebae bite off and ingest distinct host cell fragments, and that this contributes to cell killing. we review this process, termed 'amoebic trogocytosis' (trogo-, 'n ... | 2015 | 26070402 |
| potential amoebicidal activity of hydrazone derivatives: synthesis, characterization, electrochemical behavior, theoretical study and evaluation of the biological activity. | four new hydrazones were synthesized by the condensation of the selected hydrazine and the appropriate nitrobenzaldehyde. a complete characterization was done employing 1h- and 13c-nmr, electrochemical techniques and theoretical studies. after the characterization and electrochemical analysis of each compound, amoebicidal activity was tested in vitro against the hm1:imss strain of entamoeba histolytica. the results showed the influence of the nitrobenzene group and the hydrazone linkage on the a ... | 2015 | 26035095 |
| heat shock protein 90 inhibitors repurposed against entamoeba histolytica. | hsp90 is an essential chaperone responsible for trafficking a vast array of client proteins, which are substrates that hsp90 regulates in eukaryotic cells under stress conditions. the atp-binding n-terminal domain of hsp90 (also known as a ghkl type atpase domain) can serve as a specific drug target, because sufficient structural diversity in the atp-binding pocket of hsp90 allows for ortholog selectivity of hsp90 inhibitors. the primary objective of this study is to identify inhibitors specific ... | 2015 | 26029171 |
| synthesis, characterization and antiamoebic activity of chalcones bearing n-substituted ethanamine tail. | a series of chalcones (4-21) possessing n-substituted ethanamine were synthesized by the aldol condensation reaction of 1-(4-(2-substituted ethoxy)phenyl)ethanones with different aldehydes preceded by the reaction of 2-chloro n-substituted ethanamine hydrochloride and 4-hydroxy acetophenone. the structure of all the synthesized compounds was elucidated by various spectral and x-ray diffraction studies. the compounds were screened against hm1: imss strain of entamoeba histolytica and cytotoxicity ... | 2015 | 26021707 |