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mapping consistent rice (oryza sativa l.) yield qtls under drought stress in target rainfed environments.drought stress is a major limitation to rainfed rice production and yield stability. identifying yield-associated quantitative trait loci (qtls) that are consistent under drought stress predominant in target production environments, as well as across different genetic backgrounds, will help to develop high-yielding rice cultivars suitable for water-limited environments through marker-assisted breeding (mab). considerable progress has been made in mapping qtls for drought resistance traits in ric ...201526206756
physiological characteristics and related gene expression of after-ripening on seed dormancy release in rice.after-ripening is a common method used for dormancy release in rice. in this study, the rice variety jiucaiqing (oryza sativa l. subsp. japonica) was used to determine dormancy release following different after-ripening times (1, 2 and 3 months). germination speed, germination percentage and seedling emergence increased with after-ripening; more than 95% germination and 85% seedling emergence were observed following 1 month of after-ripening within 10 days of imbibition, compared with <45% germi ...201526205956
differential antioxidant responses and perturbed porphyrin biosynthesis after exposure to oxyfluorfen and methyl viologen in oryza sativa.we compared antioxidant responses and regulation of porphyrin metabolism in rice plants treated with oxyfluorfen (of) or methyl viologen (mv). plants treated with mv exhibited not only greater increases in conductivity and malondialdehyde but also a greater decline in fv/fm, compared to plants treated with of. mv-treated plants had greater increases in activities of superoxide dismutase (sod) and catalase (cat) as well as transcript levels of soda and cata than of-treated plants after 28 h of th ...201526197316
identification and expression analysis of pin-like (pils) gene family of rice treated with auxin and cytokinin.the phytohormone auxin is one of the most important signaling molecules that undergo accumulation or depletion in a temporal or spatial manner due to wide arrays of changes in developmental or stress programs. proper distribution, maintenance and homeostasis of auxin molecules across the plant systems are one of the most important phenomena required for proper growth and development of plant. the distribution and homeostasis of auxin is maintained by auxin transport systems across the plant. the ...201526193322
brassinosteroid signaling regulates leaf erectness in oryza sativa via the control of a specific u-type cyclin and cell proliferation.leaf erectness is key in determining plant architecture and yield, particularly in cereal crops. brassinosteroids (brs) play a unique role in controlling this trait in monocots, but the underlying cellular and molecular mechanisms remain big mysteries. here we report that the abaxial sclerenchyma cell number of rice lamina joints (ljs) is closely related to leaf erectness, and br signaling tightly regulates their proliferation. we identified a rice u-type cyclin cyc u4;1 enriched in rice ljs, wi ...201526190148
ethylene biosynthesis is promoted by very-long-chain fatty acids during lysigenous aerenchyma formation in rice roots.in rice (oryza sativa) roots, lysigenous aerenchyma, which is created by programmed cell death and lysis of cortical cells, is constitutively formed under aerobic conditions, and its formation is further induced under oxygen-deficient conditions. ethylene is involved in the induction of aerenchyma formation. reduced culm number1 (rcn1) is a rice mutant in which the gene encoding the atp-binding cassette transporter rcn1/osabcg5 is defective. here, we report that the induction of aerenchyma forma ...201526036614
effect of iron plaque on antimony uptake by rice (oryza sativa l.).although iron (fe) plaque has been shown to significantly affect the uptake of toxic antimony (sb) by rice, knowledge about the influence of iron plaque on antimony (sb) (amount, mechanisms, etc) is, however, limited. here, the effect of fe plaque on sb(iii) and sb(v) (nominal oxidation states) uptake by rice (oryza sativa l.) was investigated using hydroponic experiments and synchrotron-based techniques. the results showed that iron plaque immobilized sb on the surface of rice roots. although t ...201525947970
rice (oryza sativa l) plantation affects the stability of biochar in paddy soil.conversion of rice straw into biochar for soil amendment appears to be a promising method to increase long-term carbon sequestration and reduce greenhouse gas (ghg) emissions. the stability of biochar in paddy soil, which is the major determining factor of carbon sequestration effect, depends mainly on soil properties and plant functions. however, the influence of plants on biochar stability in paddy soil remains unclear. in this study, bulk and surface characteristics of the biochars incubated ...201525944542
accumulation of long-lived mrnas associated with germination in embryos during seed development of rice.mature dry seeds contain translatable mrnas called long-lived mrnas. early studies have shown that protein synthesis during the initial phase of seed germination occurs from long-lived mrnas, without de novo transcription. however, the gene expression systems that generate long-lived mrnas in seeds are not well understood. to examine the accumulation of long-lived mrnas in developing rice embryos, germination tests using the transcriptional inhibitor actinomycin d (act d) were performed with the ...201525941326
genomic distribution and possible functional roles of putative g-quadruplex motifs in two subspecies of oryza sativa.g-quadruplex is a stable, four-stranded dna or rna structure formed from guanine-rich regions and implicated in telomere maintenance, replication, gene regulation at transcription level or translation level, etc. based on bioinformatics methods, we analyzed different putative g-quadruplex motifs (pgqms) patterns in various genomic regions of two subspecies (indica and japonica) of oryza sativa and the whole genomes of other 8 species. in total, in the 10 species we discussed, the pgqms densities ...201525935116
transcriptome changes in rice (oryza sativa l.) in response to high night temperature stress at the early milky stage.rice yield and quality are adversely affected by high temperatures, especially at night; high nighttime temperatures are more harmful to grain weight than high daytime temperatures. unfortunately, global temperatures are consistently increasing at an alarming rate and the minimum nighttime temperature has increased three times as much as the corresponding maximum daytime temperature over the past few decades.201525928563
endophytic infection alleviates pb(2+) stress effects on photosystem ii functioning of oryza sativa leaves.the aims of this study were to examine the effect of pb(2+) stress on the primary reaction of photosynthesis and to assess the potential benefits of endophytic infection on the pb(2+) tolerance of rice seedlings. rice inoculated with an endophytic fungus (e+) and non-inoculated (e-) were subjected to 0, 50, 100, 150 and 200 μm pb(2+). the responses to pb(2+) stress were characterized by the analysis of chl a fluorescence. a comparison of e- with e+ rice seedlings, as evaluated by their performan ...201525885166
aquaporins in developing rice grains.during rice grain filling, grain moisture content and weight show dynamic changes. we focused on the expression of all 33 rice aquaporins in developing grains. only two aquaporin genes, ospip2;1 and ostip3;1, were highly expressed in the period 10-25 days after heading (dah). high-temperature treatment from 7 to 21 dah abolished the dynamic up-regulation of ospip2;1 in the period 15-20 dah, whereas ostip3;1 expression was not affected. immunohistochemical analysis revealed that ospip2;1 was pres ...201525884499
rice tissue-specific promoters and condition-dependent promoters for effective translational application.rice (oryza sativa) is one of the most important staple food crops for more than half of the world's population. the demand is increasing for food security because of population growth and environmental challenges triggered by climate changes. this scenario has led to more interest in developing crops with greater productivity and sustainability. the process of genetic transformation, a major tool for crop improvement, utilizes promoters as one of its key elements. those promoters are generally ...201525882130
variation and impact of drought-stress patterns across upland rice target population of environments in brazil.the upland rice (ur) cropped area in brazil has decreased in the last decade. importantly, a portion of this decrease can be attributed to the current ur breeding programme strategy, according to which direct grain yield selection is targeted primarily to the most favourable areas. new strategies for more-efficient crop breeding under non-optimal conditions are needed for brazil's ur regions. such strategies should include a classification of spatio-temporal yield variations in environmental gro ...201525873681
increased tolerance to salt stress in opda-deficient rice allene oxide cyclase mutants is linked to an increased ros-scavenging activity.salinity stress represents a global constraint for rice, the most important staple food worldwide. therefore the role of the central stress signal jasmonate for the salt response was analysed in rice comparing the responses to salt stress for two jasmonic acid (ja) biosynthesis rice mutants (cpm2 and hebiba) impaired in the function of allene oxide cyclase (aoc) and their wild type. the aoc mutants were less sensitive to salt stress. interestingly, both mutants accumulated smaller amounts of na( ...201525873666
impact of industrial effluent on growth and yield of rice (oryza sativa l.) in silty clay loam soil.degradation of soil and water from discharge of untreated industrial effluent is alarming in bangladesh. therefore, buildup of heavy metals in soil from contaminated effluent, their entry into the food chain and effects on rice yield were quantified in a pot experiment. the treatments were comprised of 0, 25%, 50%, 75% and 100% industrial effluents applied as irrigation water. effluents, initial soil, different parts of rice plants and post-harvest pot soil were analyzed for various elements, in ...201525872732
natural organic matter-induced alleviation of the phytotoxicity to rice (oryza sativa l.) caused by copper oxide nanoparticles.natural organic matter (nom) can interact with engineered nanoparticles (nps) in the environment and modify their behavior and toxicity to organisms. in the present study, the phytotoxicity of copper oxide (cuo) nps to rice seedlings in the presence of humic acid as a model nom was investigated. the results showed that cuo nps induced the inhibition of root elongation, aberrations in root morphology and ultrastructure, and losses of cell viability and membrane integrity. the adverse effects part ...201525868010
evaluation of reference genes for rt-qpcr studies in the leaves of rice seedlings under salt stress.to obtain accurate and reliable results for the expression of genes of interest using quantitative real-time polymerase chain reaction (rt-qpcr) techniques, it is necessary to normalize the data by comparing them to constitutive genes that exhibit uniform expression levels under experimental conditions. in this study, the stability of expression was evaluated for the following ten candidate reference genes in rice leaves (oryza sativa l.) from the brs bojuru and brs ligeirinho genotypes that wer ...201525867385
active site cleft mutants of os9bglu31 transglucosidase modify acceptor substrate specificity and allow production of multiple kaempferol glycosides.rice os9bglu31 is a transglucosidase that can transfer glucose to phenolic acids, flavonoids, and phytohormones. os9bglu31 displays a broad specificity with phenolic 1-o-β-d-glucose esters acting as better glucose donors than glucosides, whereas the free phenolic acids of these esters are also excellent acceptor substrates.201525863286
effect of different germination conditions on antioxidative properties and bioactive compounds of germinated brown rice.this study investigates antioxidative activity and bioactive compounds of ungerminated brown rice (ubr) and germinated brown rice (gbr). we used two rice cultivars (oryza sativa l.), taiwan japonica 9 (tj-9) and taichung indica 10 (tci-10), as the materials in our experiments. the conditions for inducing germination are soaking time in water 24, 48, or 72 h; temperature 26 or 36°c; incubation in light or darkness; and open or closed vessels, in which the antioxidative activities and bioactive co ...201525861637
morf-related gene702, a reader protein of trimethylated histone h3 lysine 4 and histone h3 lysine 36, is involved in brassinosteroid-regulated growth and flowering time control in rice.the methylation of histone h3 lysine 36 (h3k36) plays critical roles in brassinosteroid (br)-related processes and is involved in controlling flowering time in rice (oryza sativa). although enzymes that catalyze this methylation reaction have been described, little is known about the recognition mechanisms to decipher h3k36 methylation information in rice. in this study, biochemical characterizations showed that morf-related gene702 (mrg702) binds to trimethylated h3k4 and h3k36 (h3k4me3 and h3k ...201525855537
microrna399 is involved in multiple nutrient starvation responses in rice.the increasing evidences have revealed that micrornas (mirnas) play significant role in nutrient stress response. previously, mir399 was documented to be induced by phosphorus (p) starvation and involved in regulating p starvation responses. to further investigate the function of mir399 in rice (oryza sativa l.), we performed genechip analysis with osmir399 over-expressing plants. interestingly, our results showed that, besides p starvation responsive genes, the expression of a number of genes i ...201525852730
genetic analysis of fertility restoration under cgms system in rice (oryza sativa l.) using three-way test-cross method.we studied the genetics of fertility restoration by producing three-way test cross (twtc) hybrids involved different combinations of restorers, maintainers and partial restorers of rice. pollen and spikelet fertility of 16 twtc hybrids were studied. six twtc involving restorer/restorer combinations as male parents produced progenies with fertility levels ranging from complete to zero. no specific ratio of segregation was observed. the crosses involving maintainer/maintainer combinations as male ...201525846872
abiotic stress and genome dynamics: specific genes and transposable elements response to iron excess in rice.iron toxicity is a root related abiotic stress, occurring frequently in flooded soils. it can affect the yield of rice in lowland production systems. this toxicity is associated with high concentrations of reduced iron (fe(2+)) in the soil solution. although the first interface of the element is in the roots, the consequences of an excessive uptake can be observed in several rice tissues. in an original attempt to find both genes and transposable elements involved in the response to an iron toxi ...201525844118
multiple tissue-specific expression of rice seed-shattering gene sh4 regulated by its promoter psh4.rice seed shattering is an important domestication syndrome encoded by a gene named as sh4. the coding region of sh4 has been well studied regarding its function and roles in evolution. however, its promoter has not been identified, which limited our understanding of the detailed regulatory mechanisms of this gene. it is therefore critical to characterize the promoter and study its expression pattern.201525844117
ethylene responses in rice roots and coleoptiles are differentially regulated by a carotenoid isomerase-mediated abscisic acid pathway.ethylene and abscisic acid (aba) act synergistically or antagonistically to regulate plant growth and development. aba is derived from the carotenoid biosynthesis pathway. here, we analyzed the interplay among ethylene, carotenoid biogenesis, and aba in rice (oryza sativa) using the rice ethylene response mutant mhz5, which displays a reduced ethylene response in roots but an enhanced ethylene response in coleoptiles. we found that mhz5 encodes a carotenoid isomerase and that the mutation in mhz ...201525841037
characterization and immunomodulatory activity of rice hull polysaccharides.rice hulls (oryza sativa) are high in carbohydrate content and have been utilized as dietary fiber. the immunomodulatory bioactivity of rice hull polysaccharides (rhps) has rarely been reported. this study demonstrated the structural characteristics and immunomodulating of rhps. the rhps were fractioned using deae-650m column, producing one neutral and 3 acidic polysaccharide fractions. rhps were examined using hpaec-pad, hp-sec, nmr and gc-ms for structural characteristics. the results showed t ...201525839805
metabolic and transcriptomic signatures of rice floral organs reveal sugar starvation as a factor in reproductive failure under heat and drought stress.heat and drought stress are projected to become major challenges to sustain rice (oryza sativa l.) yields with global climate change. both stresses lead to yield losses when they coincide with flowering. a significant knowledge gap exists in the mechanistic understanding of the responses of rice floral organs that determine reproductive success under stress. our work connects the metabolomic and transcriptomic changes in anthers, pistils before pollination and pollinated pistils in a heat-tolera ...201525828772
genome wide association mapping for arabinoxylan content in a collection of tetraploid wheats.arabinoxylans (axs) are major components of plant cell walls in bread wheat and are important in bread-making and starch extraction. furthermore, arabinoxylans are components of soluble dietary fibre that has potential health-promoting effects in human nutrition. despite their high value for human health, few studies have been carried out on the genetics of ax content in durum wheat.201526176552
genome-wide indel markers shared by diverse asian rice cultivars compared to japanese rice cultivar 'koshihikari'.insertion-deletion (indel) polymorphisms, such as simple sequence repeats, have been widely used as dna markers to identify qtls and genes and to facilitate rice breeding. recently, next-generation sequencing has produced deep sequences that allow genome-wide detection of indels. these polymorphisms can potentially be used to develop high-accuracy polymerase chain reaction (pcr)-based markers. here, re-sequencing of 5 indica, 2 aus, and 3 tropical japonica cultivars and japanese elite cultivar ' ...201526175622
a practical protocol to accelerate the breeding process of rice in semitropical and tropical regions.breeding of excellent rice varieties is essential for modern rice production. typical breeding procedures to introduce and maintain valuable agricultural traits require at least 8 generations from crossing to stabilization, always taking more than 4-5 years of work. this long and tedious process is the rate-limiting step in the development of new varieties, and therefore fast culturing methods are in urgent need. taking advantage of early flowering characteristics of light-sensitive rice under s ...201526175620
quantitative trait loci for whiteness of cooked rice detected in improved rice cultivars in hokkaido.improving the eating quality of cooked rice has been one of the most important objectives in rice breeding programs. eating quality of cooked rice is a complex trait including several components, such as external appearance, taste, aroma, and texture. therefore, dissection of these components followed by marker-assisted selection of detected qtl(s) may be a useful approach for achieving desirable eating quality in rice breeding. whiteness of cooked rice (wcr) is an important factor related to th ...201526175616
effects of silicon (si) on arsenic (as) accumulation and speciation in rice (oryza sativa l.) genotypes with different radial oxygen loss (rol).arsenic (as) contamination of paddy soils has adversely affected the health of millions of people those consuming rice for staple food. the present study was aimed at investigating the effects of silicon (si) fertilization on as uptake, speciation in rice plants with different radial oxygen loss (rol). six genotypes were planted in pot soils under greenhouse conditions until late tillering state. the results showed that the rates of rol were higher in hybrid rice genotypes varying from 19.76 to ...201526171731
o2 dynamics in the rhizosphere of young rice plants (oryza sativa l.) as studied by planar optodes.radial o2 loss (rol) strongly affect the o2 availability in the rhizosphere of rice. the rol create an oxic zone around the roots, protecting the plant from toxic reduced chemical species and regulates the redox chemistry in the soil. this study investigates the spatio-temporal variability in o2 dynamics in the rice rhizosphere.201526166902
[effect of boron-antimony interaction on the uptake and accumulation of antimony and boron by rice seedling].effect of interactions between boron (b) and antimony on the uptake and accumulation by rice (oryza sativa l.) seedling was investigated in solution culture. the results showed that sb(iii) and sb(v) could inhibit rice growth and sb(iii) was more toxic than sb(v). concentrations of b in rice roots and shoots were significantly affected by the addition of sb(iii) and sb(v). the addition of 30 μmol x l(-1) sb(iii) could significantly decrease b of rice shoots and roots by 57.6% and 75.6%, and 30 μ ...201526164929
ss1 (nal1)- and ss2-mediated genetic networks underlying source-sink and yield traits in rice (oryza sativa l.).source leaf/sink capacity (ss) traits are important determinants of grain yield (gy) of rice. to understand the genetic basis of the ss relationship in rice, five ss and gy traits of rice were genetically dissected using two reciprocal introgression populations. seventy-three qtl affecting the ss and gy traits were identified, most of which were detected in one of the parental genetic backgrounds (gbs). two major qtl at bins 4.7 (ss1) and 3.12 (ss2) were associated consistently with all measured ...201526162098
examining two sets of introgression lines in rice (oryza sativa l.) reveals favorable alleles that improve grain zn and fe concentrations.in the modern world, the grain mineral concentration (gmc) in rice (oryza sativa l.) not only includes important micronutrient elements such as iron (fe) and zinc (zn), but it also includes toxic heavy metal elements, especially cadmium (cd) and lead (pb). to date, the genetic mechanisms underlying the regulation of gmc, especially the genetic background and g × e effects of gmc, remain largely unknown. in this study, we adopted two sets of backcross introgression lines (bils) derived from ir758 ...201526161553
response difference of transgenic and conventional rice (oryza sativa) to nanoparticles (γfe₂o₃).nanoparticles (nps) are an increasingly common contaminant in agro-environments, and their potential effect on genetically modified (gm) crops has been largely unexplored. gm crop exposure to nps is likely to increase as both technologies develop. to better understand the implications of nanoparticles on gm plants in agriculture, we performed a glasshouse study to quantify the uptake of fe2o3 nps on transgenic and non-transgenic rice plants. we measured nutrient concentrations, biomass, enzyme a ...201526154040
loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (oryza sativa l.).a genome-wide association study in rice yielded loci and candidate genes associated with tolerance to iron toxicity, and revealed biochemical mechanisms associated with tolerance in contrasting haplotypes. iron toxicity is a major nutrient disorder affecting rice. therefore, understanding the genetic and physiological mechanisms associated with iron toxicity tolerance is crucial in adaptive breeding and biofortification. we conducted a genome-wide association study (gwas) by exposing a populatio ...201526152574
global analysis reveals the crucial roles of dna methylation during rice seed development.seed development is an important process of reproductive development and consists of embryo and endosperm development; both comprise several key processes. to determine and investigate the functions of the dynamic dna methylome during seed development, we profiled the dna methylation genome wide in a series of developmental stages of rice (oryza sativa) embryo and endosperm by methylcytosine immunoprecipitation followed by illumina sequencing. the results showed that embryo is hypermethylated pr ...201526145151
a universal positive-negative selection system for gene targeting in plants combining an antibiotic resistance gene and its antisense rna.gene targeting (gt) is a useful technology for accurate genome engineering in plants. a reproducible approach based on a positive-negative selection system using hygromycin resistance and the diphtheria toxin a subunit gene as positive and negative selection markers, respectively, is now available. however, to date, this selection system has been applied exclusively in rice (oryza sativa). to establish a universally applicable positive-negative gt system in plants, we designed a selection system ...201526143254
the auxin transporter, osaux1, is involved in primary root and root hair elongation and in cd stress responses in rice (oryza sativa l.).auxin and cadmium (cd) stress play critical roles during root development. there are only a few reports on the mechanisms by which cd stress influences auxin homeostasis and affects primary root (pr) and lateral root (lr) development, and almost nothing is known about how auxin and cd interfere with root hair (rh) development. here, we characterize rice osaux1 mutants that have a longer pr and shorter rhs in hydroponic culture, and that are more sensitive to cd stress compared to wild-type (dong ...201526140668
integration of the proteome and transcriptome reveals multiple levels of gene regulation in the rice dl2 mutant.leaf vascular system differentiation and venation patterns play a key role in transporting nutrients and maintaining the plant shape, which is an important agronomic trait for improving photosynthetic efficiency. however, there is little knowledge about the regulation of leaf vascular specification and development. here we utilized the rice midribless mutant (dl2) to investigate the molecular changes in transcriptome and proteome profiles during leaf vascular specification and differentiation. u ...201526136752
increases in the longevity of desiccation-phase developing rice seeds: response to high-temperature drying depends on harvest moisture content.previous studies have suggested that the drying conditions routinely used by genebanks may not be optimal for subsequent seed longevity. the aim of this study was to compare the effect of hot-air drying and low-temperature drying on subsequent seed longevity for 20 diverse rice accessions and to consider how factors related to seed production history might influence the results.201526133688
ascorbate biosynthesis and its involvement in stress tolerance and plant development in rice (oryza sativa l.).ascorbic acid (asa) biosynthesis and its implications for stress tolerance and plant development were investigated in a set of rice knock-out (ko) mutants for asa biosynthetic genes and their wild-types. ko of two isoforms of gdp-d-mannose epimerase (osgme) reduced the foliar asa level by 20-30%, and ko of gdp-l-galactose phosphorylase (osggp) by 80%, while ko of myo-inositol oxygenase (osmiox) did not affect foliar asa levels. asa concentration was negatively correlated with lipid peroxidation ...201526129988
saline-induced changes of epicuticular waxy layer on the puccinellia tenuiflora and oryza sativa leave surfaces.the epicuticular waxy layer of plant leaves enhances the extreme environmental stress tolerance. however, the relationship between waxy layer and saline tolerance was not established well. the epicuticular waxy layer of rice (oryza sativa l.) was studied under the nahco3 stresses. in addition, strong saline tolerance puccinellia tenuiflora was chosen for comparative studies.201526129955
correction: genomic selection and association mapping in rice (oryza sativa): effect of trait genetic architecture, training population composition, marker number and statistical model on accuracy of rice genomic selection in elite, tropical rice breeding lines. 201526125618
genetic mapping of a qtl controlling source-sink size and heading date in rice.source size, sink size and heading date (hd) are three important classes of traits that determine the productivity of rice. in this study, a set of recombinant inbred lines (rils) derived from the cross between an elite indica line big grain1 (bg1) and a japonica line xiaolijing (xlj) were used to map quantitative trait loci (qtls) for source-sink size and heading date. totally, thirty-one qtls for source size, twenty-two for sink size, four for heading date and seven qtl clusters which included ...201526123916
assessment of genetic heterogeneity in structured plant populations using multivariate whole-genome regression models.plant breeding populations exhibit varying levels of structure and admixture; these features are likely to induce heterogeneity of marker effects across subpopulations. traditionally, structure has been dealt with as a potential confounder, and various methods exist to "correct" for population stratification. however, these methods induce a mean correction that does not account for heterogeneity of marker effects. the animal breeding literature offers a few recent studies that consider modeling ...201526122758
osarid3, an at-rich interaction domain-containing protein, is required for shoot meristem development in rice.the shoot apical meristem (sam) produces all of the plant's aerial organs. the sam is established either during embryogenesis or experimentally in in vitro tissue culture. although several factors including the class i knotted1-like homeobox (knoxi) proteins, auxin, and cytokinin are known to play essential roles in sam development, the underlying mechanisms of sam formation and maintenance are still largely not understood. herein we demonstrate that osarid3, a member of the rice (oryza sativa) ...201526121094
inhibition of abscission layer formation by an interaction of two seed-shattering loci, sh4 and qsh3, in rice.loss of seed shattering was one of the key phenotypic changes selected for in the domestication of many crop species. asian cultivated rice, oryza sativa l., was domesticated from its wild ancestor, o. rufipogon, and three seed-shattering loci, qsh1, sh4 and qsh3, have been reported to be involved in the loss of seed shattering in cultivated rice. here, we analysed the seed-shattering behaviour of wild rice using introgression lines carrying the cultivated alleles from o. sativa nipponbare in th ...201526119661
integrating image-based phenomics and association analysis to dissect the genetic architecture of temporal salinity responses in rice.salinity affects a significant portion of arable land and is particularly detrimental for irrigated agriculture, which provides one-third of the global food supply. rice (oryza sativa), the most important food crop, is salt sensitive. the genetic resources for salt tolerance in rice germplasm exist but are underutilized due to the difficulty in capturing the dynamic nature of physiological responses to salt stress. the genetic basis of these physiological responses is predicted to be polygenic. ...201526111541
aluminium alleviates manganese toxicity to rice by decreasing root symplastic mn uptake and reducing availability to shoots of mn stored in roots.manganese (mn) and aluminium (al) phytotoxicities occur mainly in acid soils. in some plant species, al alleviates mn toxicity, but the mechanisms underlying this effect are obscure.201526105187
rice stripe1-2 and stripe1-3 mutants encoding the small subunit of ribonucleotide reductase are temperature sensitive and are required for chlorophyll biosynthesis.we induced mutants, stripe1-2 (st1-2) and stripe1-3 (st1-3), from rice (oryza sativa l.) indica 9311 using ethyl methanesulfonate (ems). both st1-2 and st1-3 mutants encoded the small subunit of ribonucleotide reductase 1 (rnrs1), differed in the location of the mutated base, and displayed white-stripe from the l2 stage through maturity. the mutants were sensitive to temperature, and their chlorophyll content increased with the increase in temperature; however, they did not revert to normal gree ...201526102588
expression of oscas (calcium-sensing receptor) in an arabidopsis mutant increases drought tolerance.the calcium-sensing receptor (cas), which is localized in the chloroplasts, is a crucial regulator of extracellular calcium-induced stomatal closure in arabidopsis. it has homologs in oryza sativa and other plants. these sequences all have a rhodanese-like protein domain, which has been demonstrated to be associated with specific stress conditions. in this study, we cloned the oryza sativa calcium-sensing receptor gene (oscas) and demonstrated that oscas could sense an increase of extracellular ...201526098425
genetic interactions between diverged alleles of early heading date 1 (ehd1) and heading date 3a (hd3a)/ rice flowering locus t1 (rft1) control differential heading and contribute to regional adaptation in rice (oryza sativa).initiation of flowering, also called heading, in rice (oryza sativa) is determined by the florigens encoded by heading date 3a (hd3a) and rice flowering locus t1 (rft1). early heading date 1 (ehd1) regulates hd3a and rft1. however, different rice varieties have diverged alleles of ehd1 and hd3a/rft1 and their genetic interactions remain largely unclear. here we generated three segregating populations for different combinations of diverged ehd1 and hd3a/rft1 alleles, and analyzed their genetic in ...201526096631
pay1 improves plant architecture and enhances grain yield in rice.plant architecture, a complex of the important agronomic traits that determine grain yield, is a primary target of artificial selection of rice domestication and improvement. some important genes affecting plant architecture and grain yield have been isolated and characterized in recent decades; however, their underlying mechanism remains to be elucidated. here, we report genetic identification and functional analysis of the plant architecture and yield 1 (pay1) gene in rice, which affects plant ...201526095647
comparative analysis of plant lycopene cyclases.carotenoids are essential isoprenoid pigments produced by plants, algae, fungi and bacteria. lycopene cyclase (lyc) commonly cyclize carotenoids, which is an important branching step in the carotenogenesis, at one or both end of the backbone. plants have two types of lyc (β-lcy and ϵ-lcy). in this study, plant lycs were analyzed. based on domain analysis, all lycs accommodate lycopene cyclase domain (pf05834). furthermore, motif analysis indicated that motifs were conserved among the plants. on ...201526092704
rice microrna osa-mir1848 targets the obtusifoliol 14α-demethylase gene oscyp51g3 and mediates the biosynthesis of phytosterols and brassinosteroids during development and in response to stress.phytosterols are membrane components or precursors for brassinosteroid (br) biosynthesis. as they cannot be transported long distances, their homeostasis is tightly controlled through their biosynthesis and metabolism. however, it is unknown whether micrornas are involved in their homeostatic regulation. rice (oryza sativa) plants transformed with microrna osa-mir1848 and its target, the obtusifoliol 14α-demethylase gene, oscyp51g3, were used to investigate the role of osa-mir1848 in the regulat ...201526083975
molecular characterization of oryza sativa wrky6, which binds to w-box-like element 1 of the oryza sativa pathogenesis-related (pr) 10a promoter and confers reduced susceptibility to pathogens.wrky proteins are transcription factors (tfs) that regulate the expression of defense-related genes. the salicylic acid (sa)-inducible oryza sativa wrky6 (oswrky6) was identified as a positive regulator of oryza sativa pathogenesis-related 10a (ospr10a) by transient expression assays. a physical interaction between oswrky6 and w-box-like element 1 (wle1), which positively regulates ospr10a/probenazole induced protein 1 expression, was verified in vitro. several pathogenesis-related (pr) genes we ...201526083148
comprehensive phenotypic analysis of rice (oryza sativa) response to salinity stress.increase in soil salinity levels is becoming a major cause of crop yield losses worldwide. rice (oryza sativa) is the most salt-sensitive cereal crop, and many studies have focused on rice salinity tolerance, but a global understanding of this crop's response to salinity is still lacking. we systematically analyzed phenotypic data previously collected for 56 rice genotypes to assess the extent to which rice uses three known salinity tolerance mechanisms: shoot-ion independent tolerance (or osmot ...201526082319
identification of cis-elements and evaluation of upstream regulatory region of a rice anther-specific gene, osipp3, conferring pollen-specific expression in oryza sativa (l.) ssp. indica.pollen-specific expression. promoters comprise of various cis-regulatory elements which control development and physiology of plants by regulating gene expression. to understand the promoter specificity and also identification of functional cis-acting elements, progressive 5' deletion analysis of the promoter fragments is widely used. we have evaluated the activity of regulatory elements of 5' promoter deletion sequences of anther-specific gene osipp3, viz. osipp3-∆1 (1504 bp), osipp3-∆2 (968 bp ...201526081459
genetic variation in southern usa rice genotypes for seedling salinity tolerance.the success of a rice breeding program in developing salt tolerant varieties depends on genetic variation and the salt stress response of adapted and donor rice germplasm. in this study, we used a combination of morphological and physiological traits in multivariate analyses to elucidate the phenotypic and genetic variation in salinity tolerance of 30 southern usa rice genotypes, along with 19 donor genotypes with varying degree of tolerance. significant genotypic variation and correlations were ...201526074937
phloem-exudate proteome analysis of response to insect brown plant-hopper in rice.brown plant-hopper (nilaparvata lugens stål, bph), one of the most devastating agricultural insect pests of rice throughout asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. planting resistant variety is an efficient and economical way to control this pest. understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. here, we used an itraq-based quantitative proteomics approach to perform analysis of protein e ...201526072143
searching for common responsive parameters for ozone tolerance in 18 rice cultivars in india: results from ethylenediurea studies.eighteen rice (oryza sativa) cultivars were screened for ozone (o3) tolerance and for the most responsive parameters with ethylenediurea (edu) treatments at two experimental sites experiencing high ambient o3 conditions in the indo-gangetic plains (igp) of india. edu was applied at 15 day intervals until the final harvest phase as a foliar spray at 300 ppm in order to protect the plants from the adverse effects of o3. antioxidant activity, malondialdehyde content (mda), chlorophyll content, gas ...201526071964
involvement of phosphate supplies in different transcriptional regulation pathway of oryza sativa l.'s antioxidative system in response to arsenite and cadmium stress.the roles of different concentrations of arsenite (as(iii)) and cadmium (cd) (0, 25 and 50 μm) in the absence and presence (1.7 mm) of phosphate (p) in tolerance and antioxidant genes expression in oryza sativa l. were investigated. the growth parameters, metal accumulation, lipid peroxidation and soluble protein and 17 genes involved in metal accumulation and oxidative stress were measured. in our results, lsi6 (osnip2;2) could play an important role in as(iii) accumulation of shoots and roots ...201526070715
characterization and fine mapping of thermo-sensitive chlorophyll deficit mutant1 in rice (oryza sativa l.).chlorophyll content is one of the most important traits controlling crop biomass and economic yield in rice. here, we isolated a spontaneous rice mutant named thermo-sensitive chlorophyll deficit 1 (tscd1) derived from a backcross recombinant inbred line population. tscd1 plants grown normally from the seedling to tiller stages showed yellow leaves with reduced chlorophyll content, but showed no significant differences after the booting stage. at temperatures below 22°c, the tscd1 mutant showed ...201526069446
genetic diversity and population structure in aromatic and quality rice (oryza sativa l.) landraces from north-eastern india.the north-eastern (ne) india, comprising of arunachal pradesh, assam, manipur, meghalaya, mizoram, nagaland, sikkim and tripura, possess diverse array of locally adapted non-basmati aromatic germplasm. the germplasm collections from this region could serve as valuable resources in breeding for abiotic stress tolerance, grain yield and cooking/eating quality. to utilize such collections, however, breeders need information about the extent and distribution of genetic diversity present within colle ...201526067999
population genetic structure of oryza sativa in east and southeast asia and the discovery of elite alleles for grain traits.we investigated the nuclear simple sequence repeat (ssr) genotypes of 532 rice (oryza sativa l.) accessions collected from east and southeast asia and detected abundant genetic diversity within the population. we identified 6 subpopulations and found a tendency towards directional evolution in o. sativa from low to high latitudes, with levels of linkage disequilibrium (ld) in the 6 subpopulations ranging from 10 to 30 cm. we then investigated the phenotypic data for grain length, grain width, gr ...201526059752
short-term effect of caffeine on purine, pyrimidine and pyridine metabolism in rice (oryza sativa) seedlings.as part of our studies on the physiological and ecological function of caffeine, we investigated the effect of exogenously supplied caffeine on purine, pyrimidine and pyridine metabolism in rice seedlings. we examined the effect of 1 mm caffeine on the in situ metabolism of 14c-labelled adenine, guanine, inosine, uridine, uracil, nicotinamide and nicotinic acid. the segments of 4-day-old dark-grown seedlings were incubated with these labelled compounds for 6 h. for purines, the incorporation of ...201526058147
effect of caffeine on the expression pattern of water-soluble proteins in rice (oryza sativa) seedlings.it has been suggested that caffeine acts as an allelochemical which influences the germination and growth of plants. the effect of caffeine on the expression profiles of proteins was investigated in shoot-root axes of rice (oryza sativa) seedlings. two-dimensional difference gel electrophoresis combined with matrix-assisted laser desorption/ionization time of flight/time of flight mass spectrometry was employed for the separation and identification of proteins. the results indicated that amounts ...201526058146
construction of regulatory networks mediated by small rnas responsive to abiotic stresses in rice (oryza sativa).plants have evolved exquisite molecular mechanisms to adapt to diverse abiotic stresses. micrornas play an important role in stress response in plants. however, whether the other small rnas (srnas) possess stress-related roles remains elusive. in this study, thousands of srnas responsive to cold, drought and salt stresses were identified in rice seedlings and panicles by using high-throughput sequencing data. these srnas were classified into 12 categories, including "panicle_cold_down", "panicle ...201526057839
variation in nrt1.1b contributes to nitrate-use divergence between rice subspecies.asian cultivated rice (oryza sativa l.) consists of two main subspecies, indica and japonica. indica has higher nitrate-absorption activity than japonica, but the molecular mechanisms underlying that activity remain elusive. here we show that variation in a nitrate-transporter gene, nrt1.1b (osnpf6.5), may contribute to this divergence in nitrate use. phylogenetic analysis revealed that nrt1.1b diverges between indica and japonica. nrt1.1b-indica variation was associated with enhanced nitrate up ...201526053497
aba-induced ccch tandem zinc finger protein osc3h47 decreases aba sensitivity and promotes drought tolerance in oryza sativa.water deficit causes multiple negative impacts on plants, such as reactive oxygen species (ros) accumulation, abscisic acid (aba) induction, stomatal closure, and decreased photosynthesis. here, we characterized osc3h47, which belongs to ccch zinc-finger families, as a drought-stress response gene. it can be strongly induced by nacl, peg, aba, and drought conditions. overexpression of osc3h47 significantly enhanced tolerance to drought and salt stresses in rice seedlings, which indicates that os ...201526047696
rice potassium transporter oshak1 is essential for maintaining potassium-mediated growth and functions in salt tolerance over low and high potassium concentration ranges.potassium (k) absorption and translocation in plants rely upon multiple k transporters for adapting varied k supply and saline conditions. here, we report the expression patterns and physiological roles of oshak1, a member belonging to the kt/kup/hak gene family in rice (oryza sativa l.). the expression of oshak1 is up-regulated by k deficiency or salt stress in various tissues, particularly in the root and shoot apical meristem, the epidermises and steles of root, and vascular bundles of shoot. ...201526046301
salicylic acid modulates arsenic toxicity by reducing its root to shoot translocation in rice (oryza sativa l.).arsenic (as) is posing serious health concerns in south east asia where rice, an efficient accumulator of as, is prominent crop. salicylic acid (sa) is an important signaling molecule and plays a crucial role in resistance against biotic and abiotic stress in plants. in present study, ameliorative effect of sa against arsenate (as(v)) toxicity has been investigated in rice (oryza sativa l.). arsenate stress hampered the plant growth in terms of root, shoots length, and biomass as well as it enha ...201526042132
carmo: a comprehensive annotation platform for functional exploration of rice multi-omics data.high-throughput technology is gradually becoming a powerful tool for routine research in rice. interpretation of biological significance from the huge amount of data is a critical but non-trivial task, especially for rice, for which gene annotations rely heavily on sequence similarity rather than direct experimental evidence. here we describe the annotation platform for comprehensive annotation of rice multi-omics data (carmo), which provides multiple web-based analysis tools for in-depth data m ...201526040787
mads-box transcription factor osmads25 regulates root development through affection of nitrate accumulation in rice.mads-box transcription factors are vital regulators participating in plant growth and development process and the functions of most of them are still unknown. anr1 was reported to play a key role in controlling lateral root development through nitrate signal in arabidopsis. osmads25 is one of five anr1-like genes in oryza sativa and belongs to the anr1 clade. here we have investigated the role of osmads25 in the plant's responses to external nitrate in oryza sativa. our results showed that osmad ...201526258667
resequencing reveals different domestication rate for badh1 and badh2 in rice (oryza sativa).badh1 and badh2 are two homologous genes, encoding betaine aldehyde dehydrogenase in rice. in the present study, we scanned badhs sequences of 295 rice cultivars, and 10 wild rice accessions to determine the polymorphisms, gene functions and domestication of these two genes. a total of 16 alleles for badh1 and 10 alleles for badh2 were detected in transcribed region of cultivars and wild species. association study showed that badh1 has significant correlation with salt tolerance in rice during g ...201526258482
genetic variation, heritability, and diversity analysis of upland rice (oryza sativa l.) genotypes based on quantitative traits.upland rice is important for sustainable crop production to meet future food demands. the expansion in area of irrigated rice faces limitations due to water scarcity resulting from climate change. therefore, this research aimed to identify potential genotypes and suitable traits of upland rice germplasm for breeding programmes. forty-three genotypes were evaluated in a randomised complete block design with three replications. all genotypes exhibited a wide and significant variation for 22 traits ...201526258135
blsspeller: exhaustive comparative discovery of conserved cis-regulatory elements.the accurate discovery and annotation of regulatory elements remains a challenging problem. the growing number of sequenced genomes creates new opportunities for comparative approaches to motif discovery. putative binding sites are then considered to be functional if they are conserved in orthologous promoter sequences of multiple related species. existing methods for comparative motif discovery usually rely on pregenerated multiple sequence alignments, which are difficult to obtain for more div ...201526254488
overexpressing ospin2 enhances aluminium internalization by elevating vesicular trafficking in rice root apex.aluminium (al) sequestration is required for internal detoxification of al in plant cells. in this study, it was found that the rice ospin2 overexpression line (ox1) had significantly reduced al content in its cell wall and increased al concentration in cell sap only in rice root tips relative to the wild-type (wt). in comparison with wt, ox1 reduced morin staining of cytosolic al, enhanced fm 4-64 staining of membrane vesicular trafficking in root tip sections (0-1mm), and showed morin-fm 4-64 ...201526254327
analysis of modes of heat transfer in baking indian rice pan cake (dosa,) a breakfast food.heat transfer by individual modes is estimated during baking of rice (oryza sativa) pan cake (dosa), a traditional food. the mathematical expressions proposed could be used to modify the baking oven for controlling the individual modes of heat transfer to obtain the desired product texture, colour and flavour. conduction from the rotating hot plate is found to be the most prominent mode of heat transfer and is critical for obtaining the desired product characteristics such as texture and flavour ...201526243966
early senescence1 encodes a scar-like protein2 that affects water loss in rice.the global problem of drought threatens agricultural production and constrains the development of sustainable agricultural practices. in plants, excessive water loss causes drought stress and induces early senescence. in this study, we isolated a rice (oryza sativa) mutant, designated as early senescence1 (es1), which exhibits early leaf senescence. the es1-1 leaves undergo water loss at the seedling stage (as reflected by whitening of the leaf margin and wilting) and display early senescence at ...201526243619
rice tutou1 encodes a suppressor of camp receptor-like protein that is important for actin organization and panicle development.panicle development, a key event in rice (oryza sativa) reproduction and a critical determinant of grain yield, forms a branched structure containing multiple spikelets. genetic and environmental factors can perturb panicle development, causing panicles to degenerate and producing characteristic whitish, small spikelets with severely reduced fertility and yield; however, little is known about the molecular basis of the formation of degenerating panicles in rice. here, we report the identificatio ...201526243616
mapping of quantitative trait loci for contents of macro- and microelements in milled rice (oryza sativa l.).macro- and microelement contents are important traits for nutritional quality in rice. in this study, quantitative trait loci (qtls) for the contents of seven mineral elements in milled rice were detected using recombinant inbred lines (rils) of the indica rice cross zhenshan 97/milyang 46, followed by the validation and fine mapping of a qtl region on the short arm of chromosome 6. a total of 20 qtls distributed on chromosomes 1, 3, 5, 6, 10, and 11 were detected in the ril population. co-local ...201526301991
the ring-finger ubiquitin ligase haf1 mediates heading date 1 degradation during photoperiodic flowering in rice.the photoperiodic response is one of the most important factors determining heading date in rice (oryza sativa). although rhythmic expression patterns of flowering time genes have been reported to fine-tune the photoperiodic response, posttranslational regulation of key flowering regulators has seldom been elucidated in rice. heading date 1 (hd1) encodes a zinc finger transcription factor that plays a crucial role in the photoperiodic response, which determines rice regional adaptability. howeve ...201526296966
orchestration of three transporters and distinct vascular structures in node for intervascular transfer of silicon in rice.requirement of mineral elements in different plant tissues is not often consistent with their transpiration rate; therefore, plants have developed systems for preferential distribution of mineral elements to the developing tissues with low transpiration. here we took silicon (si) as an example and revealed an efficient system for preferential distribution of si in the node of rice (oryza sativa). rice is able to accumulate more than 10% si of the dry weight in the husk, which is required for pro ...201526283388
evolution of structural and functional diversification among plant argonautes.argonautes (agos) are the effector proteins of the rna-induced silencing (risc) complex, formed during the phenomena of small-rna mediated post-transcriptional gene silencing. agos are a large family of proteins; their number varies from a few (4 in chlamydomonas reinhardtii) to many (18 in oryza sativa) in plants. genetics-guided analysis have demonstrated the roles of some of the agos during growth and development of plants. biochemical studies have further revealed differences in functional s ...201526237574
differential proteomic analysis using itraq reveals alterations in hull development in rice (oryza sativa l.).rice hull, the outer cover of the rice grain, determines grain shape and size. changes in the rice hull proteome in different growth stages may reflect the underlying mechanisms involved in grain development. to better understand these changes, isobaric tags for relative and absolute quantitative (itraq) ms/ms was used to detect statistically significant changes in the rice hull proteome in the booting, flowering, and milk-ripe growth stages. differentially expressed proteins were analyzed to pr ...201526230730
analysis of stress-responsive gene expression in cultivated and weedy rice differing in cold stress tolerance.rice (oryza sativa l.) cultivars show impairment of growth in response to environmental stresses such as cold at the early seedling stage. locally adapted weedy rice is able to survive under adverse environmental conditions, and can emerge in fields from greater soil depth. cold-tolerant weedy rice can be a good genetic source for developing cold-tolerant, weed-competitive rice cultivars. an in-depth analysis is presented here of diverse indica and japonica rice genotypes, mostly weedy rice, for ...201526230579
more than one way to evolve a weed: parallel evolution of us weedy rice through independent genetic mechanisms.many different crop species were selected for a common suite of 'domestication traits', which facilitates their use for studies of parallel evolution. within domesticated rice (oryza sativa), there has also been independent evolution of weedy strains from different cultivated varieties. this makes it possible to examine the genetic basis of parallel weed evolution and the extent to which this process occurs through shared genetic mechanisms. we performed comparative qtl mapping of weediness trai ...201526031196
[transformation and mobility of arsenic in the rhizosphere and non-rhizosphere soils at different growth stages of rice].speciation and bioavailability of arsenic in the rhizosphere and non-rhizosphere soils at different growth stages (tillering stage, jointing stage, booting stage, filling stage and maturing stage) of rice (oryza sativa l.) were studied using toxicity characteristic leaching procedure (tclp) and arsenic speciation analysis. pot experiments were conducted and the soil samples were taken from a certain paddy soil in hunan province contaminated by mining industry. the results showed that: (1) with t ...201526031100
fine mapping and candidate gene analysis of qstl3, a stigma length-conditioning locus in rice (oryza sativa l.).the efficiency of hybrid seed production can be improved by increasing the percentage of exserted stigma, which is closely related to the stigma length in rice. in the chromosome segment substitute line (cssl) population derived from nipponbare (recipient) and kasalath (donor), a single cssl (sssl14) was found to show a longer stigma length than that of nipponbare. the difference in stigma length between nipponbare and sssl14 was controlled by one locus (qstl3). using 7,917 individuals from the ...201526030903
influence of the nonexchangeable potassium of mica on radiocesium uptake by paddy rice.a pot cultivation experiment was conducted to elucidate the influence of the nonexchangeable potassium (k) of mica on radiocesium ((137)cs) uptake by paddy rice (oryza sativa l. cv. koshihikari), and to evaluate the potential of mica application as a countermeasure to reduce radiocesium transfer from soil to paddy rice. the increase in the exchangeable k concentrations of soils, measured before planting, due to mica (muscovite, biotite, and phlogopite) application was negligible. however, in tri ...201526026983
osiaa6, a member of the rice aux/iaa gene family, is involved in drought tolerance and tiller outgrowth.auxin signaling is a fundamental part of many plant growth processes and stress responses and operates through aux/iaa protein degradation and the transmission of the signal via auxin response factors (arfs). a total of 31 aux/iaa genes have been identified in rice (oryza sativa), some of which are induced by drought stress. however, the mechanistic link between aux/iaa expression and drought responses is not well understood. in this study we found that the rice aux/iaa gene osiaa6 is highly ind ...201526025543
the r2r3-type myb gene osmyb91 has a function in coordinating plant growth and salt stress tolerance in rice.plants have evolved a number of different mechanisms to respond and to adapt to abiotic stress for their survival. however, the regulatory mechanisms involved in coordinating abiotic stress tolerance and plant growth are not fully understood. here, the function of osmyb91, an r2r3-type myb transcription factor of rice was explored. osmyb91 was induced by abiotic stress, especially by salt stress. analysis of chromatin structure of the gene revealed that salt stress led to rapid removal of dna me ...201526025528
the rice rcn11 gene encodes β1,2-xylosyltransferase and is required for plant responses to abiotic stresses and phytohormones.seed germination rates and plant development and growth under abiotic stress are important aspects of crop productivity. here, our characterization of the rice (oryza sativa l.) mutant reduced culm number11 (rcn11) showed that rcn11 controls growth of plants exposed to abnormal temperature, salinity and drought conditions. rcn11 also mediates root aerenchyma formation under oxygen-deficient conditions and aba sensitivity during seed germination. molecular studies showed that the rcn11 mutation r ...201526025522
constitutive expression of dacbf7, an antarctic vascular plant deschampsia antarctica cbf homolog, resulted in improved cold tolerance in transgenic rice plants.deschampsia antarctica is an antarctic hairgrass that grows on the west coast of the antarctic peninsula. in this report, we have identified and characterized a transcription factor, d. antarctica c-repeat binding factor 7 (dacbf7), that is a member of the monocot group v cbf homologs. the protein contains a single ap2 domain, a putative nuclear localization signal, and the typical cbf signature. dacbf7, like other monocot group v homologs, contains a distinct polypeptide stretch composed of 43 ...201526025521
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