Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
comparison of the microbiological quality and safety between conventional and organic vegetables sold in malaysia.given the remarkable increase of public interest in organic food products, it is indeed critical to evaluate the microbiological risk associated with consumption of fresh organic produce. organic farming practices including the use of animal manures may increase the risk of microbiological contamination as manure can act as a vehicle for transmission of foodborne pathogens. this study aimed to determine and compare the microbiological status between organic and conventional fresh produce at the ...201728824567
short communication: detection of stx2 and elt genes in bovine milk by using a multiplex pcr system.the aim of this study was to detect 2 important toxin genes from diarrheagenic escherichia coli (dec) in bovine milk using a new multiplex pcr. to standardize the multiplex pcr, the stx2 and elt genes were investigated for the detection of shiga toxin-producing escherichia coli (stec) and enterotoxigenic e. coli (etec), respectively. the dna template was prepared with a thermal procedure (boiling) and a commercial kit. samples consisted of uht and pasteurized milk, both skimmed, and stec and ete ...201728803013
synthesis and in vitro evaluation of peracetyl and deacetyl glycosides of eugenol, isoeugenol and dihydroeugenol acting against food-contaminating bacteria.essential oils, as well as their separate components, have shown promise as alternatives to synthetic preservatives. therefore, it would be interesting to optimize the effect of these compounds and to evaluate their applicability as additives in food. to this end, six peracetyl and deacetyl glycosides were synthesized from eugenol, isoeugenol and dihydroeugenol. all of the glycosides were characterized by ir and nmr. the synthesized compounds and their aglycones were evaluated to determine their ...201728763946
super-cationic carbon quantum dots synthesized from spermidine as an eye drop formulation for topical treatment of bacterial keratitis.we have developed a one-step method to synthesize carbon quantum dots (cqdpas) from biogenic polyamines (pas) as an antibacterial agent for topical treatment of bacterial keratitis (bk). cqds synthesized by direct pyrolysis of spermidine (spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. we demonstrate that cqds obtained from spds (cqdspds) possess effective antibacterial activities against non-multidrug-resista ...201728677399
antibacterial and detoxifying activity of nz17074 analogues with multi-layers of selective antimicrobial actions against escherichia coli and salmonella enteritidis.nz17074 (n1), an arenicin-3 derivative isolated from the lugworm, has potent antibacterial activity and is cytotoxic. to reduce its cytotoxicity, seven n1 analogues with different structures were designed by changing their disulfide bonds, hydrophobicity, or charge. the "rocket" analogue-n2 and the "kite" analogue-n6 have potent activity and showed lower cytotoxicity in raw264.7 cells than n1. the nmr spectra revealed that n1, n2, and n6 adopt β-sheet structures stabilized by one or two disulfid ...201728611436
effects of sorbic acid-chitosan microcapsules as antimicrobial agent on the properties of ethylene vinyl alcohol copolymer film for food packaging.this paper discusses the possibility of using sorbic acid-chitosan microcapsules (s-mps) as an antibacterial component of active ethylene vinyl alcohol copolymer (evoh) film. s-mps with a diameter of approximately 1 to 4 μm showed a sorbic acid loading capacity of 46.5%. addition of s-mps (3%, w/w) increased the tensile strength, haze, oxygen, and water vapor barrier properties, as well as reduced the elongations at break and transmittance of s-mps-evoh (s-mp-evoh) film. antibacterial tests show ...201728488775
assessment of consumer exposure to salmonella spp., campylobacter spp., and shiga toxin-producing escherichia coli in meat products at retail in the city of sao paulo, brazil.meat products may be vehicles of bacterial pathogens to humans, and salmonella spp., campylobacter spp., and shiga toxin-producing escherichia coli (stec) are the most relevant. the aim of this study was to generate data on prevalence of these three pathogens in 552 samples of meat products (hot dogs, pork sausages, raw ground beef, and raw chicken legs) sold at retail in the city of sao paulo, brazil. salmonella spp. was detected in 5.8% (32/552) of samples, comprising pork sausages 62.5% (20/3 ...201728475359
in ovo evaluation of floramax®-b11 on marek´s disease hvt vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and salmonella enteritidis infection in broiler chickens.three experiments were conducted to evaluate the effect of in ovo administration of floramax®-b11 (fm) on marek´s disease (md) herpesvirus of turkeys (hvt) vaccine protective efficacy, hatchability, microbiota composition, morphometric analysis, and salmonella enteritidis (se) infection in chickens. experiment 1 consisted of 3 trials. in trials 1 and 2, d 18 white leghorn 15i5x71 embryos were randomly distributed in 4 groups: 1) hvt vaccinated in ovo and no marek's disease virus (mdv) challenge; ...201728160004
immune response against salmonella enteritidis is unsettled by hiv infection.the human immunodeficiency virus (hiv) is responsible for more than 2 million new infections per year and opportunistic infections such as salmonella spp. gastroenteritis is an important cause of mortality and morbidity in developing countries. monocytes and macrophages play a critical role in the innate immune response against bacterial infections. however during hiv infection the virus can infect these cells and although they are more resistant to the cytopathic effects, they represent an impo ...201728447329
effect of infectious bursal disease (ibd) vaccine on salmonella enteritidis infected chickens.chickens infected with both infectious bursal disease virus (ibdv) and salmonella had higher mortality. in this work, we investigated the effect of ibdv vaccine (modified live-virus bursal disease vaccine, nobilis strain 228e®) on experimentally infected chickens with salmonella enteritidis (se).201728495316
the effect of lactobacillus acidophilus ptcc 1643 on cultured intestinal epithelial cells infected with salmonella enterica serovar enteritidis.gastrointestinal disorders caused by salmonella enterica serovar enteritidis (sese) are a significant health problem around the globe. probiotic bacteria have been shown to have positive effects on the immune responses. lactobacillus acidophilus was examined for its capability to influence the innate immune response of ht29 intestinal epithelial cells towards sese. the purpose of this work was to assess the effect of l. acidophilus ptcc 1643 on cultured intestinal epithelial cells infected with ...201728443224
selection of potential probiotic lactobacillus with inhibitory activity against salmonella and fecal coliform bacteria.three hundred and sixty presumptive lactic acid bacteria (lab) isolated from pregnant sows, newborn, suckling, and weaned piglets were preliminarily screened for anti-salmonella activity. fifty-eight isolates consisting of lactobacillus reuteri (n = 32), lactobacillus salivarius (n = 10), lactobacillus mucosae (n = 8), lactobacillus johnsonii (n = 5), and lactobacillus crispatus (n = 3) were selected and further characterized for probiotic properties including production of antimicrobial substan ...201728712023
population-based incidence, seasonality and serotype distribution of invasive salmonellosis among children in nanoro, rural burkina faso.bloodstream infections (bsi) caused by salmonella typhi and invasive non-typhoidal salmonella (ints) frequently affect children living in rural sub-saharan africa but data about incidence and serotype distribution are rare.201728692655
cxcr3 plays a critical role for host protection against salmonellosis.cxcr3 and its ligands are heavily associated with inflammation and have been implicated in numerous inflammatory diseases. cxcr3 plays an important role in recruiting pro-inflammatory cells, specifically neutrophils, in a model of sterile colitis whereby cxcr3(-/-) mice showed an attenuated course of colitis with markedly reduced host-tissue damage in the inflamed caecum. the role of cxcr3 during infectious colitis, however, is unclear and therefore in this study, we investigated the role of cxc ...201728860493
biochips for direct detection and identification of bacteria in blood culture-like conditions.bloodstream bacterial infections are life-threatening conditions necessitating prompt medical care. rapid pathogen identification is essential for early setting of the best anti-infectious therapy. however, the bacterial load in blood samples from patients with bacteremia is too low and under the limit of detection of most methods for direct identification of bacteria. therefore, a preliminary step enabling the bacterial multiplication is required. to do so, blood cultures still remain the gold ...201728842712
ground water as the source of an outbreak of salmonella enteritidis.in september 2014, an outbreak of gastroenteritis was reported to the public health institute of šibenik and knin county in croatia. the outbreak occurred in the county center of šibenik, a town with 50,000 inhabitants, and it lasted for 12days. an epidemiological investigation suggested a nearby water spring as the source of the outbreak. due to the temporary closure of the public water supply system, the inhabitants started to use untreated water from a nearby spring. microbiological analysis ...201728756827
development of a novel, rapid multiplex polymerase chain reaction assay for the detection and differentiation of salmonella enterica serovars enteritidis and typhimurium using ultra-fast convection polymerase chain reaction.salmonella enterica serovars enteritidis and typhimurium are the most common causative agents of human nontyphoidal salmonellosis. the rapid detection and timely treatment of salmonellosis are important to increase the curative ratio and prevent spreading of the disease. in this study, we developed a rapid multiplex convection polymerase chain reaction (pcr) method to detect salmonella spp. and differentiate salmonella enteritidis and salmonella typhimurium. we used the inva gene for salmonella ...201728696782
differences in expression of genes in the myd88 and trif signalling pathways and methylation of tlr4 and trif in tibetan chickens and daheng s03 chickens infected with salmonella enterica serovar enteritidis.salmonella enterica serovar (s. enteritidis) is a pathogenic bacterium that can cause symptoms of food poisoning, leading to death of poultry, resulting in serious economic losses. the myd88 and trif signalling pathways play important roles in activating innate and adaptive immunity in chickens infected with s. enteritidis. the objective of the present study was to characterize in vivo mrna expressions, protein levels and methylation levels of genes in the above two pathways in both tibetan chic ...201728669384
immunogenicity and protective efficacy of a salmonella enteritidis sptp mutant as a live attenuated vaccine candidate.salmonella enterica serovar enteritidis (s. enteritidis) is a highly adaptive pathogen in both humans and animals. as a salmonella type iii secretion system (t3ss) effector, salmonella protein tyrosine phosphatase (sptp) is critical for virulence in this genus. to investigate the feasibility of using c50336δsptp as a live attenuated oral vaccine in mice, we generated the sptp gene deletion mutant c50336δsptp in s. enteritidis strain c50336 by λ-red mediated recombination and evaluated the protec ...201728646853
sugar sulfates are not hydrolyzed by the acid-inducible sulfatase asla from salmonella enterica enteritidis nal(r) and kentucky 3795 at ph 5.5.the open reading frames sen0085 and seka_a4361, from salmonella enterica serovar enteritidis nal(r) and serovar kentucky 3795, respectively, corresponding to the acid-inducible sulfatase gene asla from salmonella enterica serovar typhimurium, were previously suggested by microarray analysis to be differentially expressed under acid conditions. however, growth and enzyme activity tests in the present study demonstrated that both wild-type strains exhibited sulfatase activity with 4-nitrophenyl su ...201728558253
global gene-expression analysis of the response of salmonella enteritidis to egg white exposure reveals multiple egg white-imposed stress responses.chicken egg white protects the embryo from bacterial invaders by presenting an assortment of antagonistic activities that combine together to both kill and inhibit growth. the key features of the egg white anti-bacterial system are iron restriction, high ph, antibacterial peptides and proteins, and viscosity. salmonella enterica serovar enteritidis is the major pathogen responsible for egg-borne infection in humans, which is partly explained by its exceptional capacity for survival under the har ...201728553268
first detection of a fosfomycin resistance gene, fosa7, in salmonella enterica serovar heidelberg isolated from broiler chickens.we previously described salmonella enterica serovar heidelberg isolates harboring a chromosomal gene cluster similar to the glutathione s-transferase gene, a putative fosa gene conferring resistance to fosfomycin. here, we show that this new gene, named fosa7, confers resistance to fosfomycin. the introduction of fosa7 into the fosfomycin-susceptible salmonella enterica serovar enteritidis resulted in a substantial increase in the fosfomycin mic. this finding increases the awareness of antibioti ...201728533247
genetic basis for loss of immuno-reactive o-chain in salmonella enterica serovar enteritidis veterinary isolates.fifty-two rough salmonella enterica serovar enteritidis (s. enteritidis) isolates from broilers and the environment were characterized for their serological and genotypic properties. under routine diagnostic serotyping methods such isolates lack the immuno-reactivity of the o-chain of the lipopolysaccharide (lps), and are referred to as non-typeable. using a modified slide agglutination method, the isolates could be differentiated into three different serological variants. twenty-six isolates (5 ...201728532797
real-time pcr method for detection of salmonella spp. in environmental samples.the methods currently used for detecting salmonella in environmental samples require 2 days to produce results and have limited sensitivity. here, we describe the development and validation of a real-time pcr salmonella screening method that produces results in 18 to 24 h. primers and probes specific to the gene inva, group d, and salmonella enterica serovar enteritidis organisms were designed and evaluated for inclusivity and exclusivity using a panel of 329 salmonella isolates representing 126 ...201728500041
urine disinfection and in situ pathogen killing using a microbial fuel cell cascade system.microbial fuel cells (mfcs) are emerging as an effective means of treating different types of waste including urine and wastewater. however, the fate of pathogens in an mfc-based system remains unknown, and in this study we investigated the effect of introducing the enteric pathogen salmonella enterica serovar enteritidis in an mfc cascade system. the mfcs continuously fed with urine showed high disinfecting potential. as part of two independent trials, during which the bioluminescent s. enterit ...201728463976
isolation and characterization of salmonella enteritidis and salmonella typhimurium from chicken meat in egypt.salmonella enterica serovars enteritidis and typhimurium represent the major serovars associated with human salmonellosis. contamination of meat products with these serovars is considered the main source of infection.201728459222
subtyping of canadian isolates of salmonella enteritidis using multiple locus variable number tandem repeat analysis (mlva) alone and in combination with pulsed-field gel electrophoresis (pfge) and phage typing.salmonella enterica subspecies enterica serovar enteritidis (se) is one of the most common causes of human salmonellosis and in canada currently accounts for over 40% of human cases. reliable subtyping of isolates is required for outbreak detection and source attribution. however, pulsed-field gel electrophoresis (pfge), the current standard subtyping method for salmonella spp., is compromised by the high genetic homogeneity of se. multiple locus variable number tandem repeat analysis (mlva) was ...201728456552
effect of dietary bacteriophage supplementation on internal organs, fecal excretion, and ileal immune response in laying hens challenged by salmonella enteritidis.with the current researches on replacing antibiotics with different dietary interventions, bacteriophages (bp) are potential antimicrobial intervention because of their ability to affect specific bacteria. a study was conducted to evaluate the role of bp against salmonella enterica serovar enteritidis (se) on se internal organs colonization and ileum immune response in laying hens. hens were challenged both orally and intracloacally with 108 cfu/ml cells of nalidixic acid resistant salmonella en ...201728453774
salmonella enterica serovar enteritidis liver abscess after blunt abdominal trauma.salmonella enterica serovar enteritidis is among the most reported serotypes of salmonella species worldwide, but rarely reported as the causative agent of a liver abscess.201728443586
an association between decreasing incidence of invasive non-typhoidal salmonellosis and increased use of antiretroviral therapy, gauteng province, south africa, 2003-2013.hiv-infected persons are at increased risk of opportunistic infections, including invasive nontyphoidal salmonella (ints) infections; antiretroviral therapy (art) reduces this risk. we explored changing ints incidence associated with increasing art availability in south africa.201728264046
sensitive detection of staphylococcus aureus with vancomycin-conjugated magnetic beads as enrichment carriers combined with flow cytometry.a novel sandwich strategy was designed to detect staphylococcus aureus. the strategy is based on an antibacterial agent that captures bacterial cells and a fluorescein-labeled antibody that acts as the signal-output probe. vancomycin (van), which exerts a strong antibacterial effect on gram-positive bacteria, was utilized as a molecular recognition agent to detect pathogenic bacteria. to effectively concentrate s. aureus, we used bovine serum albumin (bsa) as the amplification carrier to modify ...201728590745
draft genome sequences of 256 salmonella enterica subsp. enterica serovar enteritidis strains isolated from humans, food, chickens, and farm environments in brazil.salmonella enterica subsp. enterica serovar enteritidis emerged in the late 1980s as the most isolated salmonella serovar worldwide. here, we report the draft genomes of 256 s enteritidis strains isolated from humans, food, chickens, and farm environments in brazil. these draft genomes will help enhance our understanding of this serovar in brazil.201728705959
complete genome sequences of two salmonella enterica subsp. enterica serovar enteritidis strains isolated from egg products in the united states.egg-associated salmonellosis is an important public health problem in many countries. here, we report the genome sequences, including plasmids, of two strains of salmonella enterica subsp. enterica serovar enteritidis isolated from egg products in 2012 and 2013 in the united states. this will provide more information and insight into the research about egg-associated salmonellosis.201728663302
next generation sequencing-based multigene panel for high throughput detection of food-borne pathogens.contamination of food by chemicals or pathogenic bacteria may cause particular illnesses that are linked to food consumption, commonly referred to as foodborne diseases. bacteria are present in/on various foods products, such as fruits, vegetables and ready-to-eat products. bacteria that cause foodborne diseases are known as foodborne pathogens (fbps). accurate detection methods that are able to reveal the presence of fbps in food matrices are in constant demand, in order to ensure safe foods wi ...201728578266
safety, protective immunity, and diva capability of a rough mutant salmonella pullorum vaccine candidate in broilers.salmonella enterica subsp. enterica serovar gallinarum biovar pullorum (salmonella pullorum) is highly adapted to chickens causing an acute systemic disease that results in high mortality. vaccination represents one approach for promoting animal health, food safety and reducing environmental persistence in salmonella control. an important consideration is that salmonella vaccination in poultry should not interfere with the salmonellosis monitoring program. this is the basis of the diva (differen ...201728424675
dynamics of mono- and dual-species biofilm formation and interactions between staphylococcus aureus and gram-negative bacteria.microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. here, the development of mono- and dual-species biofilms formed by staphylococcus aureus and other foodborne pathogens such as salmonella enterica subsp. enterica serovar enteritidis, potentially pathogenic raoultella planticola a ...201728401747
resistance mechanisms and fitness of salmonella typhimurium and salmonella enteritidis mutants evolved under selection with ciprofloxacin in vitro.the aim of this study was to investigate the difference in resistance mechanisms and fitness of salmonella typhimurium (st) and salmonella enteritidis (se) mutants selected during the evolution of resistance under exposure to increasing ciprofloxacin concentrations in vitro. mutations in quinolone target genes were screened by pcr. phenotypic characterization included susceptibility testing by the broth dilution method, investigation of efflux activity and growth rate, and determination of the i ...201728831084
antibiotic resistance patterns and serotypes of salmonella spp. isolated at jeollanam-do in korea.few long-term studies have been conducted on the serotype and antibiotic resistance patterns of salmonella speices (spp.) the aim of this study was to determine the serotypes and antibiotic resistance patterns of salmonella spp. isolated at jeollanam-do in korea from 2004 to 2014.201728781944
outbreak of salmonella enteritidis phage type 1b associated with frozen pre-cooked chicken cubes, finland 2012.in august to october 2012, a nationwide outbreak of salmonella enteritidis phase type (pt) 1b with 53 cases occurred in finland. hypothesis generating interviews pointed toward ready-to-eat chicken salad from a finnish company and at the same time estonian authorities informed of a s. enteritidis pt 1b outbreak linked to chicken wrap prepared at an estonian restaurant. we found that chicken salad was associated with the infection (odds ratio (or) 16·1, 95% confidence interval (ci) 1·7-148·7 for ...201728770691
the novel type 1 fimbriae fimh receptor calreticulin plays a role in salmonella host specificity.it was suggested that minor differences in the structure of fimh are most likely associated with differences in its adhesion specificities and may determine the tropism of various salmonella serovars to different species and tissues. we have recently shown that fimh adhesins from host-adapted serovars, e.g., salmonella choleraesuis (sch), bind to other glycoprotein receptors compared to fimh from host-unrestricted salmonella enteritidis (se). here we identify porcine calreticulin expressed by sw ...201728770174
thoracic aortitis due to salmonella enteritidis. 201728754550
characterization of integrons and resistance genes in salmonella isolates from farm animals in shandong province, china.a total of 154 non-duplicate salmonella isolates were recovered from 1,105 rectal swabs collected from three large-scale chicken farms (78/325, 24.0%), three large-scale duck farms (56/600, 9.3%) and three large-scale pig farms (20/180, 11.1%) between april and july 2016. seven serotypes were identified among the 154 isolates, with the most common serotype in chickens and ducks being salmonella enteritidis and in pigs salmonella typhimurium. antimicrobial susceptibility testing revealed that hig ...201728747906
production of recombinant flagellin to develop elisa-based detection of salmonella enteritidis.food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. salmonella is one of the most important bacterial genera responsible for this. salmonella enteritidis (se) is one of the non-typhoid salmonellae that can be transmitted to human from poultry products, water, and contaminated food. in recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (elisa) and polymerase chain reaction (pcr) have been developed. in this study, recombi ...201728739413
outbreak of salmonella enteritidis pt14b gastroenteritis at a restaurant in england: the use of molecular typing to achieve a successful prosecution.to describe an outbreak of salmonella enteritidis phage type (pt) 14b in people who had eaten at a restaurant, and the investigation and subsequent prosecution of the food business operator (fbo).201728732304
phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by salmonella enteritidis in mice.this work aims to isolate and perform comparative studies of a phages active against a salmonella enteritidis strain from iran. also, suitable phage candidates for therapy of mice will be selected.201728702137
chronic salmonella osteomyelitis in a diabetic patient.salmonella osteomyelitis in patients without hemoglobulinopathy is quite uncommon. osteal involvement is seen in only 0.8% of all salmonella infection cases. we describe the case of a 67-year-old diabetic woman who developed salmonella osteomyelitis and subsequently underwent a surgical excision of a tibial lesion followed by two months of intravenous (iv) antibiotic therapy. the patient responded very well to the treatment. this case is exemplary for the successful treatment of chronic salmonel ...201728680773
contribution of flagella and motility to gut colonisation and pathogenicity of salmonella enteritidis in the chicken.salmonella enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. the role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. in this study, one-day-old chickens were challenged orally with a wild-type strain salmonella enteritidis, a non-motile but fully flagellated (se δmotb) or non-flagellated (se δflic) strain to evaluate their ability to coloni ...201728648636
dna aptamer-based colorimetric detection platform for salmonella enteritidis.food safety is a major issue to protect public health and a key challenge is to find detection methods for identification of hazards in food. food borne infections affects millions of people each year and among pathogens, salmonella enteritidis is most widely found bacteria causing food borne diseases. therefore, simple, rapid, and specific detection methods are needed for food safety. in this study, we demonstrated the selection of dna aptamers with high affinity and specificity against s. ente ...201728646719
differential protein expression in chicken macrophages and heterophils in vivo following infection with salmonella enteritidis.in this study we compared the proteomes of macrophages and heterophils isolated from the spleen 4 days after intravenous infection of chickens with salmonella enteritidis. heterophils were characterized by expression of mmp9, mrp126, lect2, cathl1, cathl2, cathl3, lyg2, lyz and rsfr. macrophages specifically expressed receptor proteins, e.g. mrc1l, lrp1, lgals1, lrpap1 and a dmbt1l. following infection, heterophils decreased alb and fn1, and released mmp9 to enable their translocation to the sit ...201728623956
different roles of cd4, cd8 and γδ t-lymphocytes in naive and vaccinated chickens during salmonella enteritidis infection.lymphocytes represent the key antigen-specific leukocyte subpopulation. despite their importance in mounting an immune response, an unbiased description of proteins expressed by chicken lymphocytes has not been presented. in this study, we therefore intravenously infected chickens with salmonella enteritidis, sorted cd4, cd8 and γδ t-lymphocytes from the spleen by flow cytometry and determined the proteome of each population by lc-ms/ms. cd4 t-lymphocyte characteristic proteins included ubiquiti ...201728621911
multi-country outbreak of salmonella enteritidis infection linked to the international ice hockey tournament.in april 2015, finnish public health authorities alerted european union member states of a possible multi-country salmonella enteritidis outbreak linked to an international youth ice-hockey tournament in latvia. the european centre for disease prevention and control (ecdc), finnish and latvian authorities initiated an outbreak investigation to identify the source. the investigation included a description of the outbreak, retrospective cohort study, microbiological investigation and trace-back. w ...201728610640
multilevel mycotic aneurysms due to salmonella infection: case report and review of the literature.infected or mycotic aneurysms (mas) of the aorta and adjacent arteries are rare and difficult to treat. we report a unique case of a salmonella serotype enteritidis-induced rapidly expanding aortic and iliac pseudoaneurysm during preoperative workup. based on the presented case, we postulate that the agressive nature of salmonella enteritidis mas should not be underestimated. if postponed intervention is chosen and the patient is managed conservatively with antibiotic therapy to create a window ...201728602900
outer membrane vesicles derived from salmonella enteritidis protect against the virulent wild-type strain infection in a mouse model.foodborne contamination and salmonellosis caused by salmonella enteritidis (s. enteritidis) are a significant threat to human health and poultry enterprises. outer membrane vesicles (omvs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (lps), outer membrane proteins (omps), and phospholipids, as well as periplasmic components. in the present study, we purified omvs derive ...201728539041
magnetic nanoparticle-enhanced pcr for the detection and identification of staphylococcus aureus and salmonella enteritidis.this paper evaluated magnetic nanoparticle-enhanced pcr for the detection and identification of staphylococcus aureus and salmonella enteritidis. two different types of magnetic nanoparticles designated mpio (iron concentration 2.5 mg/ml, size 1 μm) and np (iron concentration 8.7 mg/ml, size 60 nm), both conjugated with s. aureus or s. enteritidis antibodies were evaluated as an enrichment procedure for pcr-detection of the pathogens in trypticase soy broth, milk, blood and meat broth. bacterial ...201728513808
phenotypic and genotypic characterization of salmonella spp. isolated from foods and clinical samples in brazil.increasing antimicrobial resistance in salmonella species has been a serious problem for public health worldwide. this study examines salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and pfge genotypes. it identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. salmonella infantis (16.5%) and salmonella enteritidis (13.7%) are the most common among food isolates, whereas salmonella enteriti ...201728513778
phenolic composition, anti-inflammatory, antioxidant, and antimicrobial activities of alchemilla mollis (buser) rothm.the current study was designed to evaluate the antioxidant, anti-inflammatory and antimicrobial activities of alchemilla mollis (buser) rothm. (rosaceae) aerial parts extracts. chemical composition was analyzed by spectrophotometric and chromatographic (hplc) techniques. the antioxidant properties assessed included dpph(·) and abts(·+) radical scavenging, β-carotene-linoleic acid co-oxidation assay. antimicrobial activity was evaluated with disc diffusion and micro dilution method. in order to e ...201728502116
frequency and duration of fecal shedding of salmonella enteritidis by experimentally infected laying hens housed in enriched colony cages at different stocking densities.human infections with salmonella enteritidis are often attributed to the consumption of contaminated eggs, so the prevalence of this pathogen in egg-laying poultry is an important public health risk factor. numerous and complex environmental influences on salmonella persistence and transmission are exerted by management practices and housing facilities used in commercial egg production. in recent years, the animal welfare implications of poultry housing systems have guided the development of alt ...201728443289
quinolone resistance determinants of clinical salmonella enteritidis in thailand.salmonella enteritidis has emerged as a global concern regarding quinolone resistance and invasive potential. although quinolone-resistant s. enteritidis has been observed with high frequency in thailand, information on the mechanism of resistance acquisition is limited. to elucidate the mechanism, a total of 158 clinical isolates of nalidixic acid (nal)-resistant s. enteritidis were collected throughout thailand, and the quinolone resistance determinants were investigated in the context of resi ...201728437229
experimental induction of chicken amyloid a amyloidosis in white layer chickens by inoculation with inactivated vaccines.we investigated the amyloidogenic potential of inactivated vaccines and the localized production of serum amyloid a (saa) at the injection site in white layer chickens. hens in the treated group were injected intramuscularly three times with high doses of inactivated oil-emulsion salmonella enteritidis vaccine and multivalent viral and bacterial inactivated oil-emulsion vaccines at two-week intervals. chickens in the control group did not receive any inoculum. in the treated group, emaciation an ...201728421832
heat stress decreases expression of the cytokines, avian β-defensins 4 and 6 and toll-like receptor 2 in broiler chickens infected with salmonella enteritidis.a high ambient temperature is a highly relevant stressor in poultry production. heat stress (hs) has been reported to reduce animal welfare, performance indices and increase salmonella susceptibility. salmonella spp. are major zoonotic pathogen that cause over 1 billion of human infections worldwide annually. therefore, the current study was designed to analyze the effect of heat stress on salmonella infection in chickens through modulation of the immune responses. salmonella enteritidis was ino ...201728413046
cocaine- and amphetamine-regulated transcript peptide (cart) in the nerve fibers of the porcine gallbladder wall under physiological conditions and after salmonella enteritidis lipopolysaccharides administration.cocaine- and amphetamine-regulated transcript (cart) peptide is the substance distributed in various parts of the nervous system. the majority of previous studies described this substance in the brain, where it takes part in regulatory processes connected with the food intake. cart is also present in the peripheral nervous system, especially in the enteric neurons and nerves located in the wall of the stomach and intestine, but many aspects of distribution and functions of this peptide in the di ...201728394007
salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods.the performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of pcr primers to detected salmonella spp. contamination in layer hen farms. also, the association of farm characteristics with salmonella presence was evaluated. environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. salmonella spp. was most detected in ...201728379493
molecular characterisation of salmonella strains isolated from outbreaks and sporadic cases of diarrhoea occurred in paraná state, south of brazil.a total of 46 strains of salmonella isolated from patients with sporadic diarrhoea or involved in foodborne outbreaks were analysed by pcr for genus identification and serotyping. subtyping was performed using pulsed-field gel electrophoresis (pfge) and multiple amplification of phage locus typing (maplt) for seven variable loci. bacteria were identified as belonging to serotype enteritidis (33 strains; 71·7%) or typhimurium (13 strains; 28·3%). a high similarity coefficient (94·6%) was observed ...201728367777
erratum: distinct salmonella enteritidis lineages associated with enterocolitis in high-income settings and invasive disease in low-income settings. 201728358127
mannan- and xylooligosaccharides modulate caecal microbiota and expression of inflammatory-related cytokines and reduce caecal salmonella enteritidis colonisation in young chickens.salmonella enteritidis is a pathogen, which can infect humans and chickens. this study was designed to address the impact of two potential prebiotics, mannanoligosaccharides (mos) and xylooligosaccharides (xos), on the caecal microbiota and expression of cytokines in chickens infected with s. enteritidis. newly hatched chicks were assigned to one of five groups: (1) uninfected control, (2) infected control, (3) infected + xos, (4) infected + mos and (5) infected + virginiamycin. the number of s. ...201727810877
prospective use of whole genome sequencing (wgs) detected a multi-country outbreak of salmonella enteritidis.since april 2015, whole genome sequencing (wgs) has been the routine test for salmonella identification, surveillance and outbreak investigation at the national reference laboratory in england and wales. in may 2015, an outbreak of salmonella enteritidis cases was detected using wgs data and investigated. uk cases were interviewed to obtain a food history and links between suppliers were mapped to produce a food chain network for chicken eggs. the association between the food chain network and t ...201727780484
fatal salmonellosis in captive maras (dolichotis patagonum) caused by salmonella enteritidis.we report epidemic occurrences of fatal salmonellosis caused by salmonella enteritidis in captive maras (dolichotis patagonum) in a zoological garden in japan. the deaths were sudden or followed a peracute course within a few hours of the first observations of clinical abnormalities. gross lesions included haemorrhages in the subcutis and skeletal muscles, liver, spleen, lung, heart and gastrointestinal tract. microscopically, there were haemorrhagic and necrotizing lesions with gram-negative ba ...201728238470
changes in thermo-tolerance and survival under simulated gastrointestinal conditions of salmonella enteritidis pt4 and salmonella typhimurium pt4 in chicken breast meat after exposure to sequential stresses.this study assessed changes in thermo-tolerance and capability to survive to simulated gastrointestinal conditions of salmonella enteritidis pt4 and salmonella typhimurium pt4 inoculated in chicken breast meat following exposure to stresses (cold, acid and osmotic) commonly imposed during food processing. the effects of the stress imposed by exposure to oregano (origanum vulgare l.) essential oil (oveo) on thermo-tolerance were also assessed. after exposure to cold stress (5°c for 5h) in chicken ...201728380343
distribution and antimicrobial susceptibility of foodborne salmonella serovars in eight provinces in china from 2007 to 2012 (except 2009).one thousand four hundred ninety-one salmonella isolates recovered from retail foods including chicken, beef, fish, pork, dumplings, and cold dishes in china in 2007, 2008, 2010, 2011, and 2012 were analyzed for distribution of serotype and antimicrobial susceptibility. a total of 129 salmonella serotypes were detected among 1491 isolates. salmonella enteritidis (21.5%), typhimurium (11.0%), indiana (10.8%), thompson (5.4%), derby (5.1%), agona (3.8%), and shubra (3.0%) were the seven most impor ...201728375673
interventions to reduce the bacterial load in recycled broiler litter.two experiments were undertaken to evaluate the bacterial load in recycled litter between broiler flocks following addition of quicklime (t1), windrowing (t2), shallow fermentation (t3), and control (no intervention, t4). the first experiment was developed in field conditions in which the broiler houses were accompanied by 6 consecutive flocks and the effect of the treatments was assessed on enterobacteria and aerobic mesophiles. the second experiment was conducted in an experimental broiler hou ...201728371809
allelic variation in tlr4 is linked to resistance to salmonella enteritidis infection in chickens.salmonella enteritidis (se) is a foodborne pathogen that negatively affects both animal and human health. polymorphisms of the tlr4 gene may affect recognition by toll-like receptor 4 (tlr4) of bacterial lipopolysaccharide (lps), leading to differences in host resistance to pathogenic infections. the present study has investigated polymorphic loci of chicken tlr4 (chtlr4) in ten chicken breeds, electrostatic potentials of mutant structures of tlr4, and a linkage analysis between allelic variatio ...201728339850
nuggets of wisdom: salmonella enteritidis outbreaks and the case for new rules on uncooked frozen processed chicken.in 2014 and 2015, three canadian salmonella serotype enteritidis outbreak investigations implicated uncooked, frozen, processed chicken products produced at the same establishment, namely establishment a. in november 2014, a sustained increase in the number of reported domestically acquired salmonella enteritidis cases in ontario led to the first outbreak investigation, which implicated uncooked, frozen, processed chicken products produced at establishment a. in june 2015, the identification of ...201728338343
molecular detection and antimicrobial resistance profile of zoonotic salmonella enteritidis isolated from broiler chickens in kohat, pakistan.salmonella enteritidis infection is a frequently encountered zoonotic problem, occurring with concerning regularity in recent years on a worldwide basis. the study that we undertook for the first time detected s. enteritidis and associated antimicrobial resistance pattern in broiler chickens.201728286058
evaluation of a multiplex pcr assay for the identification of salmonella serovars enteritidis and typhimurium using retail and abattoir samples.a multiplex pcr was developed to identify the two most common serovars of salmonella causing foodborne illness in canada, namely, serovars enteritidis and typhimurium. the pcr was designed to amplify dna fragments from four salmonella genes, namely, inva gene (211-bp fragment), irob gene (309-bp fragment), typhimurium stm 4497 (523-bp fragment), and enteritidis se147228 (612-bp fragment). in addition, a 1,026-bp ribosomal dna (rdna) fragment universally present in bacterial species was included ...201728221989
salmonella enteritidis flagellar mutants have a colonization benefit in the chicken oviduct.egg borne salmonella enteritidis is still a major cause of human food poisoning. eggs can become internally contaminated following colonization of the hen's oviduct. in this paper we aimed to analyze the role of flagella of salmonella enteritidis in colonization of the hen's oviduct. using a transposon library screen we showed that mutants lacking functional flagella are significantly more efficient in colonizing the hen's oviduct in vivo. a micro-array analysis proved that transcription of a nu ...201728131374
effective characterization of salmonella enteritidis by most probable number (mpn) followed by multiplex polymerase chain reaction (pcr) methods.nontyphoidal salmonella (nts) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. in this study, we determined the capacity to combine the most probable number (mpn) and multiplex polymerase chain reaction (pcr) methods to characterize the most important salmonella serotypes in raw sewage. a total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex pcr methods. from those, 123 isolates were selected based on pcr band ...201727987122
acyl homoserine lactone changes the abundance of proteins and the levels of organic acids associated with stationary phase in salmonella enteritidis.quorum sensing (qs) is cell-cell communication mechanism mediated by signaling molecules known as autoinducers (ais) that lead to differential gene expression. salmonella is unable to synthesize the ai-1 acyl homoserine lactone (ahl), but is able to recognize ahls produced by other microorganisms through sdia protein. our study aimed to evaluate the influence of ai-1 on the abundance of proteins and the levels of organic acids of salmonella enteritidis. the presence of n-dodecyl-homoserine lacto ...201727916690
descriptive survey and salmonella surveillance of pastured poultry layer farms in california.while pasture-raised poultry comprises a small portion of the commercial poultry industry in north america, these alternative rearing systems have become increasingly popular. as such, it is critical to improve our understanding of husbandry practices and prevalence of zoonotic and epizoonotic diseases in these systems. this research reviews the results of a survey sent to 82 commercial pastured poultry farms in california. while the survey response was low (13.4%), it was enhanced by detailed i ...201727744297
multi-laboratory validation study of multilocus variable-number tandem repeat analysis (mlva) for salmonella enterica serovar enteritidis, 2015.multilocus variable-number tandem repeat analysis (mlva) is a rapid and reproducible typing method that is an important tool for investigation, as well as detection, of national and multinational outbreaks of a range of food-borne pathogens. salmonella enterica serovar enteritidis is the most common salmonella serovar associated with human salmonellosis in the european union/european economic area and north america. fourteen laboratories from 13 countries in europe and north america participated ...201728277220
signature-tagged mutagenesis screening revealed a novel smooth-to-rough transition determinant of salmonella enterica serovar enteritidis.salmonella enterica serovar enteritidis (s. enteritidis) has emerged as one of the most important food-borne pathogens for humans. lipopolysaccharide (lps), as a component of the outer membrane, is responsible for the virulence and smooth-to-rough transition in s. enteritidis. in this study, we screened s. enteritidis signature-tagged transposon mutant library using monoclonal antibody against somatic o9 antigen (o9 mab) and o9 factor rabbit antiserum to identify novel genes that are involved in ...201728253852
public health significance of major genotypes of salmonella enterica serovar enteritidis present in both human and chicken isolates in korea.salmonella enterica serovar enteritidis is one of the most common serotypes implicated in salmonella infections in both humans and poultry worldwide. it has been reported that human salmonellosis is mainly associated with the consumption of poultry products contaminated with serovar enteritidis. the present study was to extensively analyze the public health risk of serovar enteritidis isolates from chickens in korea. a total of 127 chicken isolates were collected from clinical cases, on-farm fec ...201728242576
induction of viable but nonculturable salmonella in exponentially grown cells by exposure to a low-humidity environment and their resuscitation by catalase.salmonella is a major cause of foodborne disease that sometimes occurs in massive outbreaks around the world. this pathogen is tolerant of low-humidity conditions. we previously described a method for induction of viable but nonculturable (vbnc) salmonella enterica serovar enteritidis by treatment with hydrogen peroxide (h2o2) and subsequent resuscitation with 0.3 mm sodium pyruvate. here, we report a new method for the induction of the vbnc state in salmonella enteritidis cells, one involving d ...201728221986
whole-genome sequence and annotation of salmonella enterica subsp. enterica serovar enteritidis phage type 8 strain en1660.the genome of salmonella enterica subspecies enterica serovar enteritidis phage type 8 strain en1660, isolated from an outbreak in thunder bay, canada, was sequenced to 46-fold coverage using an illumina miseq with 300-bp paired-end sequencing chemistry to produce 28 contigs with an n50 value of 490,721 bp.201728126943
cecal micrornaome response to salmonella enterica serovar enteritidis infection in white leghorn layer.salmonella enterica serovar enteritidis (se) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. micrornas (mirnas) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. the objective of this study is to identify mirnas associated with se infection in laying chicken cecum.201728086873
complete genome sequence of the salmonella enterica serovar enteritidis bacteriophages fse1c and fse4c isolated from food matrices.salmonella enterica serovar enteritidis is one of the most common causes of salmonellosis worldwide. utilization of bacteriophages as prophylactic agents is a practical solution to prevent salmonellosis in ready-to-eat products. shelf stability is one of the desirable properties for prophylactic bacteriophages. here, we describe the phenotype, genome, and phylogeny of fse1c and fse4s salmonella bacteriophages. fse1c and fse4s were previously isolated from pickle sauce and ground beef respectivel ...201728074119
chitosan/sulfated locust bean gum nanoparticles: in vitro and in vivo evaluation towards an application in oral immunization.this work proposes the design of nanoparticles based on locus bean gum (lbg) and chitosan to be used as oral immunoadjuvant for vaccination purposes. lbg-based nanoparticles were prepared by mild polyelectrolyte complexation between chitosan (cs) and a synthesized lbg sulfate derivative (lbgs). morphological characterization suggested that nanoparticles present a solid and compact structure with spherical-like shape. sizes around 180-200nm and a positive surface charge between +9mv and +14mv wer ...201728049014
acyl homoserine lactone-based quorum sensing stimulates biofilm formation by salmonella enteritidis in anaerobic conditions.quorum sensing regulates a variety of phenotypes in bacteria including the production of virulence factors. salmonella spp. have quorum sensing systems mediated by three autoinducers (ai-1, ai-2, and ai-3). the ai-1-mediated system is incomplete in that the bacterium relies on the synthesis of signaling molecules by other microorganisms. this study aimed to evaluate the influence of the ai-1 n-dodecanoyl-dl-homoserine lactone (c12-hsl) on the growth, motility, adhesion, and biofilm formation of ...201727838734
evaluation of a selected lactic acid bacteria-based probiotic on salmonella enterica serovar enteritidis colonization and intestinal permeability in broiler chickens.two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (floramax-b11(®)) against salmonella enterica serovar enteritidis intestinal colonization and intestinal permeability in broiler chickens. experiment 1 consisted of two independent trials. in each trial, day-old broiler chicks were assigned to one of two groups: control + s. enteritidis or probiotic + s. enteritidis. at 72 h post-s. enteritidis challenge, haematology and caecal content were evaluated ...201727545145
spread of a major clone of salmonella enterica serotype enteritidis in poultry and in salmonellosis outbreaks in southern brazil.salmonella spp. are among the most important agents of foodborne diseases all over the world. human salmonella outbreaks are often associated with the consumption of poultry products (meat and eggs), and one of the most prevalent serotypes associated with these products is salmonella enteritidis. brazil is one of the most important poultry exporters in the world. in southern brazil, three closely related clones of salmonella enteritidis have been responsible for the majority of foodborne salmone ...201728221880
characterization of o-antigen delivered by generalized modules for membrane antigens (gmma) vaccine candidates against nontyphoidal salmonella.invasive nontyphoidal salmonella disease (ints) is a leading cause of death and morbidity in africa. the most common pathogens are salmonella enterica serovars typhimurium and enteritidis. the o-antigen portion of their lipopolysaccharide is a target of protective immunity and vaccines targeting o-antigen are currently in development. here we investigate the use of generalized modules for membrane antigens (gmma) as delivery system for s. typhimurium and s. enteritidis o-antigen. gram-negative b ...201727998639
an efficient multiplex pcr-based assay as a novel tool for accurate inter-serovar discrimination of salmonella enteritidis, s. pullorum/gallinarum and s. dublin.salmonella enterica serovars enteritidis, pullorum/gallinarum, and dublin are infectious pathogens causing serious problems for pig, chicken, and cattle production, respectively. traditional serotyping for salmonella is costly and labor-intensive. here, we established a rapid multiplex pcr method to simultaneously identify three prevalent salmonella serovars enteritidis, pullorum/gallinarum, and dublin individually for the first time. the multiplex pcr-based assay focuses on three genes tcps, ly ...201728360901
transfer of campylobacter and salmonella from poultry meat onto poultry preparation surfaces.thermophilic campylobacter and salmonella enterica are major causes of gastrointestinal foodborne infection. survival of these pathogens on food-associated surfaces is a risk contributing to their spread through the food system. this study examined the transfer of two strains each of c. jejuni , c. coli , salmonella enteritidis, and salmonella typhimurium from chicken meat to a knife or scissors used on either a plastic or wooden cutting board. each strain of campylobacter and salmonella at ∼10( ...201728358259
investigation of salmonella enteritidis outbreak associated with truffle oil - district of columbia, 2015.on september 8, 2015, the district of columbia department of health (dcdoh) received a call from a person who reported experiencing gastrointestinal illness after eating at a district of columbia (dc) restaurant with multiple locations throughout the united states (restaurant a). later the same day, a local emergency department notified dcdoh to report four persons with gastrointestinal illness, all of whom had eaten at restaurant a during august 30-september 5. two patients had laboratory-confi ...201728301453
immunogenicity of a bivalent adjuvanted glycoconjugate vaccine against salmonella typhimurium and salmonella enteritidis.salmonella enterica serovars typhimurium and enteritidis are the predominant causes of invasive non-typhoidal salmonella (ints) disease. considering the co-endemicity of s. typhimurium and s. enteritidis, a bivalent vaccine formulation against both pathogens is necessary for protection against ints disease, thus investigation of glycoconjugate combination is required. in the present work, we investigated the immune responses induced by s. typhimurium and s. enteritidis monovalent and bivalent gl ...201728289411
killing of staphylococcus aureus and salmonella enteritidis and neutralization of lipopolysaccharide by 17-residue bovine lactoferricins: improved activity of trp/ala-containing molecules.bovine lactoferricin (lfcinb) has potent antibacterial, antifungal and antiparasitic activities but is also hemolytic. our objective was to identify lfcinb17-31 derivatives with reduced hemolysis and improved antimicrobial activity via substituting cys3, arg4, gln7, met10, and gly14 with more hydrophobic residues. two peptides, lfcin4 and lfcin5, showed higher activity against staphylococcus aureus and salmonella enteritidis and lower hemolytic activity than the parent peptide lfcinb17-31. these ...201728287172
Refresh
firstprev
...6162
nextlast
Displaying items 6101 - 6195 of 6195