Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| o-nitro-paraphenylenediamine in hair dye - an unusual dental hazard. | 2002 | 139262 | |
| the uptake in the pancreatic islets of nicotinamide, nicotinic acid and tryptophan and their ability to prevent streptozotocin diabetes in mice. | the uptake of the nicotinamide adenine dinucleotide (nad)-precursors nicotinamide, nicotinic acid and tryptophan in the pancreatic islets of mice was studied by use of autoradiographic methods. the ability of these substances to prevent streptoxotocin diabetes was studied in the same species. it was found that only nicotinamide was strongly accumulated in the pancreatic islets and nicotinamide was also the only nad-precursor which protected against the streptoxotocin diabetes. apparently there i ... | 2002 | 135465 |
| [nutrition of the population of kazakhstan]. | basic results of research on the problem of nutrition in kazakhstan over the whole period of the soviet system are reported. in the republic main attention was focussed on the study of the actual dietary pattern of the population in different geographic zones. the results of investigations served as a background to proposals aimed at rationalizing the dietary for the population of kazakhstan, at working out standard rates of the foodstuffs consumption, approved by the state planning commission o ... | 2002 | 135413 |
| on the origin of the supernumerary chromosome in autosomal trisomies--with special reference to down's syndrome. a bias in tracing nondisjunction by chromosomal and biochemical polymorphisms. | the differential staining methods for chromosomes have led to the demonstration of more chromosomal polymorphisms. not rarely, these polymorphisms allow in autosomal trisomies the detection of parental origin of the supernumerary chromosome. in addition, the malsegregation may be ascribed to 1st or 2nd meiotic division in informative families. this approach of analyzing possible causes of trisomies is subject to a considerable bias. trisomic phenotypes are twice as frequent for 2nd meiotic error ... | 2002 | 133081 |
| a vascular ureterogram. | 2002 | 132979 | |
| [prof. b.v. kliucharev (on his 70th birthday)]. | 2002 | 135392 | |
| progestational agents and blood coagulation. vii. thromboembolic and other complications of oral contraceptive therapy in relationship to pretreatment levels of blood coagulation factors: summary report of a ten-year study. | during a ten-year period, 348 women were studied for a total of 5,877 patient months in four separate studies relating oral contraceptives to changes in hematologic parameters. significant increases in certain factors of the blood coagulation and fibrinolysin systems (factors i,ii,vii,viii,ix, and x and plasminogen) were observed in the treated groups. severe complications developed in four patients. all four had an abnormal blood coagulation profile, suggesting "hypercoagulability" before initi ... | 2002 | 133615 |
| endometriosis in the black woman. | endometriosis has heretofore been considered rare in black women. laparoscopy in 190 black women, in many of whom pelvic inflammatory disease was previously diagnosed clinically and treated, revealed endometriosis to be present in 21 per cent. the importance of this finding for clinical practice is discussed. | 2002 | 133613 |
| details of glycosaminoglycan conformations and intermolecular interactions. | among the mucopolysaccharides, similarities of chemical constitution are manifested in the similar molecular conformations that can be assumed by most of them. the differences, however, result in a preference for particular conformations. small amounts of one glycosaminoglycan in a mixture can change the conformational preference of the major component. advances in the techniques of fiber diffraction analysis allow us to visualize the roles of water and ions in some of these structures and to ra ... | 2002 | 137130 |
| [effects of diazepam on six drug-induced locomotor hyperactivities in mice (author's transl)]. | experiments were carried out in mice to investigate the influence of diazepam (dzp) on dexamphetamine, parachloro-n-methylamphetamine (pcma), cocaine, morphine, trihexyphenidyl or (in maois pretreated) reserpine induced motor hyperactivity. the interaction of dzp with these hyperactivities in which probably different biochemical central mechanisms are involved allows to construct a profile of action of dzp and to approach its mechanism of action. the locomotor hyperactivities induced by dexamphe ... | 2002 | 129788 |
| the care of deaf schoolchildren and other handicapped in nigeria. | 2002 | 128767 | |
| [effect of alumina, used in the electrometallurgy of aluminum, on the air environment of electrolytic shops]. | 2002 | 126195 | |
| [recent pathogenetic and biochemical findings in down's disease]. | 2002 | 129299 | |
| [the efficacy of ventilation systems in hospitals (author's transl)]. | 2002 | 135217 | |
| [laparoscopy in diagnosis of retroperitoneal non-organ tumors]. | 2002 | 128136 | |
| [electrocochleography as a neurootological tool (author's transl)]. | a series of 13 patients where surgery confirmed retrocochlear lesions in the cerebellopontine angle was tested preoperatively by electro-cochleography. the following conclusions can be drawn: 1. compound action potentials (cap) of the acoustic nerve could be registered by electro-cochleography in all patients regardless of the fact whether hearing on the side of the lesion could be demonstrated in pure tone audiometry or not. 2. the threshold latency for the 2 khz sinustone-burst elicited compou ... | 2002 | 135156 |
| [report on clinical positive evidence of the nerve excitability test in facial palsy (author's transl)]. | comparative studies of case controls of bell's palsy, posttraumatic and postoperative palsies in relation to the clinical positive evidence of the nerve excitability test are demonstrated. while an absolutely parallel reaction of clinical function restitution and electrical excitability is observed in bell's palsy and in posttraumatic late palsy, on the other hand this congruence will not be observed in postoperative palsies (in intact anatomical conduction of the nerve) in most of the investiga ... | 2002 | 135143 |
| [pseudarthrosis of the dorso-lumbar vertebrae]. | the authors have observed three cases of fractures at the thoraco-lumbar level which did not unite after ten to twelve months - dynamic x-rays demonstrated persisting mobility of about 20 degrees. the fracture line of the vertebral bodies was situated in a frontal plane and fragments were separated by the antero-inferior edge of the above vertebral body. the authors conclude that such fractions ought to be treated by internal fixation when recent, internal fixation and grafting when ancient. | 2002 | 128053 |
| [possibilities of paraclinical exploration of periodontal vascularization]. | 2002 | 128046 | |
| [sedlacková's syndrome]. | 2002 | 127301 | |
| [hypoglycemia in children]. | 2002 | 120590 | |
| [concentrations of cefradine in renal tissue (author's transl)]. | after a single bolus injection of two grams of cefradine the concentrations of antibiotic in serum and renal tissues of 21 patients were estimated. after 30 minutes the mean serum concentrations were 159 microgram/ml, after 60 mins 62 microgram/ml and after 95 mins 22 microgram/ml. the tissue concentrations in normal renal tissue (group i) after 30 minutes were 348 microgram/ml and with tissue of chronically inflamed kidneys (group ii) 118 microgram/ml in the same period. after 90 minutes the co ... | 2002 | 117321 |
| [point classification of hypertension and electrocardiographic characteristics of the left heart ventricle hypertrophy and vascular fundus changes as well as some ophthalmodynamometric measurements]. | 2002 | 124362 | |
| [parameters of digitally averaged potentials of individual motor units]. | of sixty normal subjects of different age six different muscles each were examined electromyographically. the activity pattern was recorded for moderate voluntary innervation and superposed digitally. the parameters of digitally averaged summation action potentials were compared with those of manually assessed potentials. the two methods gave identical results: the duration of potentials was between 5 and 10 ms. it increased in the younger and older age groups and showed only slight changes in m ... | 2002 | 128025 |
| letter: suggestions for improving multidiscipline health care. | 2002 | 127977 | |
| correlation of enzymatic activity, muscle myoglobin concentration and lung morphology with activity metabolism in snakes. | in vitro activity of the anaerobic enzymes phosphofructokinase and lactic dehydrogenase from axial muscle tissue of coluber constrictor, crotalus viridis and lichanura roseofusca was shown to correlate with levels of lactate production during activity by these snakes. additionally, gross and histological lung structure and axial muscle myoglobin concentration were investigated in these species. coluber was shown to have the most complex lung structure and highest muscle myoglobin content. these ... | 2002 | 135039 |
| sliding filaments in sperm flagella. | 2002 | 127892 | |
| (na+, k+)-atpase activity and sterol composition of membranes. | 2002 | 140325 | |
| [intensive neuroleptic treatment in schizophrenias]. | a report is made on the first results of the treatment with droperidol of subacute illnesses of the schizophrenic type. droperidol is a quick-working, very potent neuroleptic which leads to rapid comfort in cases of psychomotor conditions of agitation. the idea is discussed that droperidol tends to accumulate and, beyond mere sedation, produces an excessive anti-psychotic effect which provides a favourable medium for the further introduction of other neuroleptics. | 2002 | 122458 |
| the family and the young handicapped child: the importance of the right start. | 2002 | 139531 | |
| [relative biological efficiency of neutrons (2 mev) and x-rays for mastocytoma cell division capacity]. | the effects of 2 mev neutrons were compared with those of 200 kv x-rays for the criterion of lethality in mastocytoma cells of ascites type. the cells prepared as suspension were exposed to the radiations in vitro under aerobic condition and transplanted in the abdominal cavity of mice. their viability was estimated according to the growing speed of the excreted amount of urinary 5-hiaa in the host animal. the rbe varied between a value of 3.1 at the 10 per cent level of cell survival to 1.8 for ... | 2002 | 134823 |
| [cestodes of the genera gyrocoelia fuhrmann, 1899 and infula burt, 1939, 1939 (acoleidae) from the snipes of the soviet union]. | the data are given on the morphology of cestodes of the genera gyrocoelia fuhrm,. 1899 and infula burt, 1939. it has been found that in the spermatheca of the female of g. perversa there are spermatozoids with an elongated head typical for plathelminthes. in the spermatheca of the female of i. burhini were found spermatids with long tails. the final formation of spermatozoids in i. burhini takes place in the spermatheca. the taxonomic position of the species of gyrocoelia found in the waters of ... | 2002 | 139584 |
| [organization of the work of the specialized interdistrict branches]. | 2002 | 135448 | |
| problems of classification of low-back pain. | 2002 | 139668 | |
| [instrumental perforations of the esophagus]. | 2002 | 139654 | |
| erythrocyte enzymes in myelomatosis. | the activity of the erythrocyte enzymes: glucose-6-phosphate dehydrogenase, pyruvate kinase, glutathion reductase and atpase were measured in 8 patients with untreated myelomatosis. glucose-6-phosphate dehydrogenase was significantly increased. glucose-6-phosphate dehydrogenase values were negatively correlated with the glomerular filtration rate as measured by 51cr-edta clearance. the results support the existence of a shortened red cell survival in peripheral blood related to the degree of ren ... | 2002 | 135347 |
| [a case of coexisting descending thoracic aortic aneurysm and atypical aortic coarctation treated successfully by surgery using the thromboexclusion method]. | the thromboexclusion method was successfully applied to a 43-year-old male with aortitis syndrome. preoperative aortogram showed a fusiform aneurysm of the descending aorta just below the left subclavian artery and atypical coarctation of the descending aorta distal to this aneurysm. pressure gradient across the stenosis was about 70 mmhg. the technique of flow reversal and thromboexclusion was performed in this patient because of severe calcification in the aortic arch and the entire descending ... | 2002 | 2732543 |
| paracoccidioidal infection in the wife of a patient with paracoccidioidomycosis. | three agar immunoprecipitin techniques (double immunodiffusion, immunoelectrophoresis and immunoelectroosmophoresis-immunodiffusion) made with paracoccidioidin and serum of a paracoccidioidomycosis patient's wife, permitted us to observe a specific band in 6 successive samples of blood taken from the wife over a period of 21 months. the case represents an example of a subclinical paracoccidioidomycosis infection that is usually diagnosed in its disseminated, progressive form. three possible sour ... | 2002 | 120614 |
| [prevention of pustular diseases of textile workers in tashkent]. | 2002 | 135117 | |
| [articular fractures of the calcaneum. therapeutic indications based on a series of 105 cases]. | the authors have treated 105 articular fractures of the calcaneum. three methods were tested: open reduction and fixation, conservative treatment with early mobilization, primary subtalar arthrodesis. the final results were satisfactory in 85% of the cases. however the three above groups were hardly comparable. details on the radiological study of these fractures are given. the authors conclude that when osteosynthesis obtained a perfect anatomical reduction, functional results were good. but pe ... | 2002 | 128052 |
| [traumatic dislocations of the hip with fracture of the femur head. apropos of 28 cases]. | having reviewed 28 cases of fractures of the femoral head complicating traumatic dislocation of the hip the authors point out that the problems to be solved are the reduction of the dislocation (close or surgical) and the fate of the femoral head fragment. close reduction succeeded in 12 cases, failed in 12 other cases, was not attempted 4 times. open reduction was performed 16 times. when the femoral fragment was small, close reduction was attempted and the femoral head fragment neglected (10 c ... | 2002 | 128050 |
| ontogenetic patterns of frequency-power spectra elicited by flickering light in children: a comparative study of evoked and background eegs. | frequency-power spectra of the eeg evoked by repeptitive photic stimulation and of the background eeg were studied during childhood in 43 awake subjects aged between 2 months and 14 years. eeg activity was recorded from the middle parieto-occipital region with the aid of a 1-channel analyzer lysograf-alvar, analysing 16 frequencies in the range from 2 to 28 c/sec. the responsiveness of the central nervous system to flickering light improved in the course of childhood in parallel with the signifi ... | 2002 | 128017 |
| a differential scanning calorimetric-thin-layer chromatographic study of 5-halo-2'-deoxynucleosides. | 2002 | 135000 | |
| [medical synchrocyclotron proton tract of the leningrad institute of nuclear physics]. | 2002 | 127916 | |
| laparoscopy in hepatic disease. | 2002 | 134923 | |
| [angio-immunoblastic lymphoadenopathy with dysproteinemia (author's transl)]. | 2002 | 120279 | |
| [on micro-environment of active sites of myosin atpase: studies with chromophoric and fluorescent atp analogs (author's transl)]. | 2002 | 153370 | |
| disabled living foundation. | 2002 | 127995 | |
| [round table on vasodilator agents in congestive heart failure]. | 2002 | 119894 | |
| [legislation in the field of occupational dermatoses (author's transl)]. | 2002 | 137781 | |
| opacities and hypoplasia in the enamel of burmese children from a low fluoride area. | 2002 | 130393 | |
| [details of a denounced physician (e.n.t.) suspected of bodily injury, and the following expert judgement (author's transl)]. | an otologist was denounced and the patient's case history was confiscated because after tonsillectomy an absecess and a thrombosis of the vena jugularis interna had developed. the procedure and the medical aspect how at first being judged by the lawyer and the public prosecutor are described. on the base of the given medical opinion the court quashed proceedings. because of the growing number of denouncements this casuistic paper ends with four guiding principles to protect against unjustified m ... | 2002 | 135139 |
| [use of toothpastes and mouthwashes as supplementary aids in the treatment of dentin hyperesthesia localized in the cervical region of the teeth]. | 2002 | 128040 | |
| editorial: antibiotics in acne. | 2002 | 127946 | |
| receptor and interneuron light-adaptation in the dragonfly visual system. | intracellular recordings show that the receptors and second-order interneurons of the dragonfly compound eye change their sensitivity in response to maintained illumination. comparison of receptor with interneuron shows that neural mechanisms act to ensure that the modulation of interneuron membrane potential that is set up by contrast changes is independent of background intensity. | 2002 | 125980 |
| [virus antigen-antibody complex]. | 2002 | 127857 | |
| coagulation studies in leptospirosis. | coagulation and serum f.d.p. studies in 10 leptospirosis patients (5, l. bataviae, 2, l. autumnalis, 2, l. australis, and 1, l. akiyami a.)showed prolongation of prothrombin time, partial thromboplastin time and thrombin clotting time, slight low platelet count, depletion of factor v and slightly high serum f.d.p. in only four cases. four cases had bleeding and one case died because of severe haemorrhage but with only slight changes in coagulation factors and slightly high serum f.d.p. these fin ... | 2002 | 1084021 |
| [efficiency evaluation of combined intervention measures with improving drinking water first to prevent infantile acute diarrhea]. | the paper analysed factors of acute diarrhea in children under five by mantel-haenszel stratification and logistic regression. the total person-years of observation were 1332. the results showed that the combined risk factors which caused diarrhea were drinking the pond water and failure to wash hands before eating and so on. the combined intervention measures, especially improvement of drinking water had effectively decreased the incidence rate of infantile acute diarrhea through the observatio ... | 2002 | 1782659 |
| centrometric linkage in down's syndrome. | 2002 | 127693 | |
| construction of a trivalent candidate vaccine against shigella species with dna recombination. | in this work asd gene of shigella flexneri 2a strain t32 was replaced by vibrio cholerae toxin b subunit (ctxb) gene with dna recombination in vivo and in vitro. the resulting derivative of t32, designed as fwl01, could stably express ctxb, but its growth in lb medium depended on the presence of diaminopimelic acid (dap). then form i plasmid of shigella sonnei strain s7 was labeled with strain t32 asd gene and mobilized into fwl01. thus a trivalent candidate oral vaccine strain, designed as fsw0 ... | 2002 | 18763059 |
| effects of the bacteriocin psvp-10 produced by pseudomonas sp. on sensitive bacterial strains. | the bacteriocin psvp-10 is a 2.6 kda peptide which was isolated and purified from pseudomonas sp. this bacteriocin possesses lethal activity over enterococcus faecalis, salmonella typhimurium and shigella flexneri. the experimental assays showed that the bacteriocin is able to be adsorbed by all cells of these bacterial species and also by their isolated cell walls. it was observed that the resistant mutants and their respective cell walls are unable to adsorb the bacteriocin. assays performed w ... | 2002 | 17061511 |
| determination of the profile of fatty acids of 4 species of shigella spp by chromatography of gases. | shigella boydii, shigella flexneri, shigella dysenteriae and shigella sonnei were identified using gas chromatography instead of the traditional techniques. their acid methyl esters profiles were determined using a gas chromatograph hewlett packard 5890a and a rsl-150 heliflex capillary column. a total of 192 samples were analyzed both reference strains (atcc 8700, indre b2188, b2194 and b2199) and environmental isolates. 12 fatty acids were included in the profiles from which 3-hydroxytetradeca ... | 2002 | 17063774 |
| preservation of in vitro biological functions in regional lymph node lymphocytes in squamous head and neck cancer. | regional lymph node lymphocytes from patients with squamous cancer of the head and neck were tested in vitro for their ability to proliferate in response to phytohemagglutinin, concanavalin a, and allogeneic stimuli in one-way mixed lymphocyte culture. their ability to act as cytotoxic effectors in phytohemagglutinin-dependent cellular cytotoxicity was also evaluated, and all results were compared to normal lymph node or blood lymphocytes. the regional lymph node lymphocytes retained proliferati ... | 2002 | 139203 |
| [development, function and equipment of the new building of the department of dentistry]. | 2002 | 135439 | |
| comparative clinical pharmacokinetics of tubocurarine, gallamine, alcuronium and pancuronium. | data about plasma concentration, renal and biliary elimination of tubocurarine, gallamine, alcuronium and pancuronium are taken from the literature for synoptical pharmacokinetic analysis. all plasma concentration curves can be described by triexponential functions according to an open three-compartment pharmacokinetic model in line, the elements of which are an application and measuring compartment, a pharmacologically specific compartment and a non-specific one. the corresponding results for a ... | 2002 | 150804 |
| chemical hazards in the workplace. | 2002 | 135215 | |
| [hermetic closure of liplike fistulas during laparoscopic gastrostomy and jejunostomy]. | the author has elaborated the method of additional hermetical closing of labioid fistulas of the stomach and jejunum on the basis of creation of a fibrous ring in edges of an exterior fistular orifice. in case of impaired hermeticity this ring allows permanent bearing of a cannula and hermetic closure of the fistula again. doubling of the methods of hermetical closure of fistulas provided an opportunity to facilitate the technic of their creation up to a simple bringing out of the visceral wall ... | 2002 | 128191 |
| prophylactic treatment of angina pectoris. a double-blind cross-over comparison of alprenolol and pentanitrol. | twenty-one patients with angina pectoris took part in a double-blind cross-over comparison of alprenolol (an adrenergic beta-blocker), pentanitrol (pentaerythritol tetranitrate), and placebo. two-thirds of the patients were clinically improved on alprenolol 100 mg. four times daily, which was found to be significantly better than pentanitrol 30 mg. four times daily and placebo. there was also an indication of reduced severity of anginal attacks during alprenolol therapy. it is concluded that suc ... | 2002 | 4391702 |
| the anterior pituitary and hypothalamic hormones. | 2002 | 127779 | |
| [what are the results of the federal emission legislation?]. | 2002 | 124034 | |
| [development of hematology and transfusiology in uzbekistan]. | 2002 | 135253 | |
| [disabled children in the federal republic of germany in 1974 (author's transl)]. | within the scope of an additional micro-census inquiry--carried out in 1974--the federal statistical office in wiesbaden assessed the number of physically, mentally and psychologically impaired children. the results are differentiated according to the types of disability and therapy, age, in-patient treatment and special school attendance. furthermore, the estimated results are compared with earlier surveys of the federal statistical office and other estimates. | 2002 | 135318 |
| pulled elbow. | 2002 | 129456 | |
| [role of immune complex in cancer immunity]. | 2002 | 127862 | |
| spa32 regulates a switch in substrate specificity of the type iii secreton of shigella flexneri from needle components to ipa proteins. | type iii secretion systems (ttss) are essential virulence determinants of many gram-negative bacteria and serve, upon physical contact with target cells, to translocate bacterial proteins directly across eukaryotic cell membranes. the shigella ttss is encoded by the mxi/spa loci located on its virulence plasmid. by electron microscopy secretons are visualized as tripartite with an external needle, a transmembrane domain, and a cytoplasmic bulb. in the present study, we generated a shigella spa32 ... | 2002 | 12057936 |
| human monocytes kill shigella flexneri but then die by apoptosis associated with suppression of proinflammatory cytokine production. | shigella flexneri infection of human macrophages is followed by rapid bacterial escape into the cytosol and secretion of ipab, which activates caspase-1 to mediate cell death and release of mature interleukin (il)-1 beta. here we report a different outcome following infection of human peripheral blood monocytes. s. flexneri infects monocytes inefficiently in the absence of complement and, following complement-dependent uptake, cannot escape the endosomal compartment. consequently, bacteria are k ... | 2002 | 12065527 |
| shigella flexneri regulates tight junction-associated proteins in human intestinal epithelial cells. | shigella spp. are a group of gram-negative enteric bacilli that cause acute dysentery in humans. we demonstrate that shigella flexneri has evolved the ability to regulate functional components of tight junctions after interaction at the apical and basolateral pole of model intestinal epithelia. in the regulation of tight junctional protein assemblies, s. flexneri can engage serotype-specific mechanisms, which targets not only expression, but also cellular distribution and membrane association of ... | 2002 | 12067320 |
| [development of vaccine for enterohemorrhagic escherichia coli infection]. | efforts on the development of vaccines against enterohemorrhagic escherichia coli (ehec) infection has been described in this review. two kinds of vaccines were developed and these have been targeted for in humans and cattle. one vaccine candidate is toxoid, which uses an inactive form of shiga toxin(stx). a part of b subunit, each b or a subunit or one or two amino acid mutated holotoxin were developed as a toxoid vaccine candidate. the other candidate was bacterial surface antigen such as a li ... | 2002 | 12078077 |
| phenotypic and genotypic characterization of serologically atypical strains of shigella flexneri type 4 isolated in dhaka, bangladesh. | twenty-one atypical shigella flexneri type 4 strains isolated from patients attending the dhaka treatment center of the international centre for diarrhoeal disease research, bangladesh, were extensively characterized and compared with s. flexneri serotypes 4a and 4b. the atypical strains agglutinated only with the type antigen factor 4 and did not agglutinate with any group factors, thereby excluding their characterization into serotype 4a or 4b. of the 21 strains, 85.7% did not ferment mannitol ... | 2002 | 12089268 |
| prevalence of the genes for shigella enterotoxins 1 and 2 among clinical isolates of shigella in israel. | two enterotoxins, shigella enterotoxin 1 (shet1) and shigella enterotoxin 2 (shet2) have been recently characterized and are believed to play a role in the clinical manifestation of shigellosis. one hundred and twenty-one isolates of shigella spp. of 13 different serotypes and variants and 10 isolates of enteroinvasive escherichia coli (eiec) isolated in israel, were examined by polymerase chain reaction for the presence of shet1 and shet2 genes. shet1 was only prevalent among isolates of s. fle ... | 2002 | 12113500 |
| identification of chromosomal shigella flexneri genes induced by the eukaryotic intracellular environment. | upon entry into the eukaryotic cytosol, the facultative intracellular bacterium shigella flexneri is exposed to an environment that may necessitate the expression of particular genes for it to survive and grow intracellularly. to identify genes that are induced in response to the intracellular environment, we screened a library containing fragments of the s. flexneri chromosome fused to a promoterless green fluorescent protein gene (gfp). bacteria containing promoter fusions that had a higher le ... | 2002 | 12117948 |
| [a fatal case of shigellosis in an elderly patient]. | a 71 year-old male, with no recent history of travelling abroad and a past history of lung cancer two years prior to presentation, which had been successfully treated, developed a sudden onset of watery diarrhea more than ten times a day on february 26, 2001, which gradually became bloody. the next day he visited the department of integrated medicine of the tokyo metropolitan komagome hospital by ambulance because his consciousness was deteriorating and he was hospitalized. he was hypotensive on ... | 2002 | 12136656 |
| quadrupole ion-trap mass spectrometry to locate fatty acids on lipid a from gram-negative bacteria. | the structure of lipid a released by mild acid hydrolysis from lipopolysaccharide from two strains of shigella flexneri with different degrees of acylation was characterized using electrospray ionization (esi) and ion-trap mass spectrometry. the lipid a was analyzed underivatized with esi in negative-ion mode. with multiple stages of fragmentation (ms(n)), both the degree of acylation and the positions of the fatty acids on the disaccharide backbone could be determined. it was possible to determ ... | 2002 | 12137789 |
| mxie regulates intracellular expression of factors secreted by the shigella flexneri 2a type iii secretion system. | the mxi-spa locus on the virulence plasmid of shigella flexneri encodes components of the type iii secretion system. mxie, a gene within this locus, encodes a protein that is homologous to the arac/xyls family of transcriptional regulators, but currently its role in pathogenesis remains undefined. we characterized the virulence phenotype of a nonpolar mxie mutant and found that this mutant retained the ability to invade mammalian cells in tissue culture and secrete ipas (type iii effectors requi ... | 2002 | 12142411 |
| rapid detection of six types of bacterial pathogens in marine waters by multiplex pcr. | a rapid multiplex pcr (m-pcr) method that allows the simultaneous detection, in a single tube, of six commonly encountered waterborne pathogens is developed. the target genes used were: the aerolysin (aero) gene of aeromonas hydrophila, the invasion plasmid antigen h (ipah) gene of shigella flexneri, the attachment invasion locus (ail) gene of yersinia enterocolitca, the invasion plasmid antigen b (ipab) gene of salmonella typhimurium, the enterotoxin extracellular secretion protein (epsm) gene ... | 2002 | 12146868 |
| isolation of shigella dysenteriae type 1 and s. flexneri strains from surface waters in bangladesh: comparative molecular analysis of environmental shigella isolates versus clinical strains. | bacillary dysentery caused by shigella species is a public health problem in developing countries including bangladesh. although, shigellae-contaminated food and drinks are often the source of the epidemic's spread, the possible presence of the pathogen and transmission of it through environmental waters have not been adequately examined. we analyzed surface waters collected in dhaka, bangladesh, for the presence of shigellae by a combination of pcr assays followed by concentration and culturing ... | 2002 | 12147489 |
| bacterial inactivation by high-pressure homogenisation and high hydrostatic pressure. | the resistance of five gram-positive bacteria, enterococcus faecalis, staphylococcus aureus, lactobacillus plantarum, listeria innocua and leuconostoc dextranicum, and six gram-negative bacteria, salmonella enterica serovar typhimurium, shigella flexneri, yersinia enterocolitica, pseudomonas fluorescens and two strains of escherichia coli, to high-pressure homogenisation (100-300 mpa) and to high hydrostatic pressure (200-400 mpa) was compared in this study. within the group of gram-positive bac ... | 2002 | 12160080 |
| identification of a putative pathogenicity island in shigella flexneri using subtractive hybridisation of the s. flexneri and escherichia coli genomes. | the genetic differences between the human pathogen, shigella flexneri, and the non-pathogenic escherichia coli were investigated in an attempt to identify pathogenicity islands (pais) in the s. flexneri genome. genomic subtraction identified a large unique region of dna which was present in s. flexneri serotype 2a but absent from e. coli k-12. this 42-kb dna segment was localised to the s. flexneri chromosome and was found to contain a number of elements often associated with pais including: ins ... | 2002 | 12167547 |
| acid tolerance of shigella sonnei and shigella flexneri. | determination of the behaviour of shigella sonnei and sh. flexneri under acid conditions. | 2002 | 12174047 |
| enteroinvasive escherichia coli virulence-plasmid-carried apyrase (apy) and ospb genes are organized as a bicistronic operon and are subject to differential expression. | in shigella flexneri and enteroinvasive escherichia coli (eiec) the expression of the virulence-plasmid(pinv)-carried potential pathogenesis-associated apy gene, which encodes apyrase (atp diphosphohydrolase), is regulated by the same regulators that govern the expression of virulence genes. to understand the transcriptional organization of the apy gene, the authors sequenced an 8023 bp psti fragment of the pinv of eiec strain hn280, which encompasses apy as well as its adjacent genes. the psti ... | 2002 | 12177345 |
| initial steps of shigella infection depend on the cholesterol/sphingolipid raft-mediated cd44-ipab interaction. | shigellosis is an acute inflammatory bowel disease caused by the enteroinvasive bacterium shigella: upon host cell-shigella interaction, major host cell signalling responses are activated. deciphering the initial molecular events is crucial to understanding the infectious process. we identified a molecular complex involving proteins of both the host, cd44 the hyaluronan receptor, and shigella, the invasin ipab, which partitions during infection within specialized membrane microdomains enriched i ... | 2002 | 12198147 |
| sequence analysis and characterization of plasmid psfd10 from salmonella choleraesuis. | the nucleotide sequence of a small plasmid, designated psfd10, is isolated from the vaccine strain salmonella choleraesuis c500 in china, has been determined. this plasmid is 4091 bp long with a total g+c content of 51.4%, which is in the range of salmonella genomic dna. analysis of the complete nucleotide sequence reveals that psfd10 has a high degree of similarity to cole1-type plasmid, having the possible cer and rom genes, and a putative mobilization origin of cole1-type. plasmid psfd10 poss ... | 2002 | 12206756 |
| three modifications in the d and t arms of trna influence translation in escherichia coli and expression of virulence genes in shigella flexneri. | the modified nucleosides 2'-o-methylguanosine, present at position 18 (gm18), 5-methyluridine, present at position 54 (m(5)u54), and pseudouridine, present at position 55 (psi55), are located in the d and t arms of trnas and are close in space in the three-dimensional (3d) structure of this molecule in the bacterium escherichia coli. the formation of these modified nucleosides is catalyzed by the products of genes trmh (gm18), trma (m(5)u54), and trub (psi55). the combination of trmh, trma, and ... | 2002 | 12218021 |
| [phenomenon of apoptosis in the survival of enterobacteria in the parasite-host system]. | data on the apoptosis phenomenon with enterobacteria used as a model are presented. one of the mechanisms regulating the vital activity of eukaryotic cells is, together with cell proliferation and differentiation, the phenomenon known as "apoptosis". this physiological process of the eukaryotic cells death is used by many parasites in parasite--host relationships in different epitopes. the system known to trigger programmed cell death, is the surface receptor fas, the receptor of tumor necrosis ... | 2002 | 11949266 |
| neural wiskott-aldrich syndrome protein (n-wasp) is the specific ligand for shigella virg among the wasp family and determines the host cell type allowing actin-based spreading. | shigella, the causative agent of bacillary dysentery, is capable of directing its movement within host cells by forming an actin comet tail. the virg (icsa) pro-tein expressed at one pole of the bacterium recruits neural wiskott-aldrich syndrome protein (n-wasp), a member of the wasp family, which in turn stimulates actin-related protein (arp) 2/3 complex-mediated actin polymerization. as all the wasp family proteins induce actin polymerization by recruiting arp2/3 complex, we investigated their ... | 2002 | 11952639 |
| spa15 of shigella flexneri, a third type of chaperone in the type iii secretion pathway. | the type iii secretion (tts) pathway is used by numerous gram-negative pathogens to inject virulence factors into eukaryotic cells. in addition to a functional tts apparatus, secretion of effector proteins depends upon specific chaperones. using a two-hybrid screen in yeast and a co-purification assay in shigella flexneri, we demonstrated that spa15, which is encoded by an operon for components of the tts apparatus, is associated in the cytoplasm with three proteins that are secreted by the tts ... | 2002 | 11952903 |
| regulation of transcription by the activity of the shigella flexneri type iii secretion apparatus. | the virulence plasmid-encoded type iii secretion system of shigella flexneri consists of the mxi-spa secretion apparatus, secreted proteins ipaa-d and ipgd involved in entry of bacteria into epithelial cells,cytoplasmic chaperones ipgc and ipge and 15 other secreted proteins of unknown function, including vira and members of the ipah family. the activity of the mxi-spa apparatus is regulated by external signals, and transcription of vira and ipah genes is specifically induced in conditions of ac ... | 2002 | 11971264 |
| genomic subtraction to identify and characterize sequences of shiga toxin-producing escherichia coli o91:h21. | to identify shiga toxin-producing escherichia coli genes associated with severe human disease, a genomic subtraction technique was used with hemolytic-uremic syndrome-associated o91:h21 strain ch014 and o6:h10 bovine strains. the method was adapted to the shiga toxin-producing e. coli genome: three rounds of subtraction were used to isolate dna fragments specific to strain ch014. the fragments were characterized by genetic support analysis, sequencing, and hybridization to the genome of a collec ... | 2002 | 11976103 |
| characterization of pecl18 and pkpn2: a proposed pathway for the evolution of two plasmids that carry identical genes for a type ii restriction-modification system. | the primary structures of the plasmids pecl18 (5571 bp) and pkpn2 (4196 bp) from escherichia coli and klebsiella pneumoniae, respectively, which carry genes for a type ii restriction-modification system (rms2) with the specificity 5'-ccngg-3', were determined in order to elucidate the structural relationship between them. the data suggest a possible role for recombination events at bom (basis of mobility) regions and the sites of resolution of multimer plasmid forms (so-called cer sequences) in ... | 2002 | 11976960 |
| quantification of shigella icsa required for bacterial actin polymerization. | shigella move through the cytoplasm of host cells by active polymerization of host actin to form an "actin tail." actin tail assembly is mediated by the shigella protein icsa. the process of shigella actin assembly has been studied extensively using icsa-expressing escherichia coli in cytoplasmic extracts of xenopus eggs. however, for reasons that have been unclear, wild type shigella does not assemble actin in these extracts. we show that the defect in actin assembly in xenopus extracts by shig ... | 2002 | 11977093 |