Publications

diallyl disulfide, a chemopreventive agent in garlic, induces multidrug resistance-associated protein 2 expression.	the organosulfur compounds (oscs), present in garlic, are studied for their protective effect against human cancers. p-glycoprotein (p-gp) and multidrug resistance protein 2 (mrp2) are two transporters involved in the defense of cells and in the development of multidrug resistance. whereas oscs increase glutathione s-transferase activity (gst), mrp2 plays a role in the transport of glutathione (gsh)-conjugates. in this study, we have investigated the effect of two oscs, diallyl disulfide (dads) ...	2004	15474518
human mutl homolog (mlh1) function in dna mismatch repair: a prospective screen for missense mutations in the atpase domain.	germline mutations in the dna mismatch repair (mmr) genes msh2 and mlh1 are responsible for the majority of hereditary non-polyposis colorectal cancer (hnpcc), an autosomal-dominant early-onset cancer syndrome. genetic testing of both msh2 and mlh1 from individuals suspected of hnpcc has revealed a considerable number of missense codons, which are difficult to classify as either pathogenic mutations or silent polymorphisms. to identify novel mlh1 missense codons that impair mmr activity, a prosp ...	2004	15475387
a high resolution protein interaction map of the yeast mediator complex.	mediator is a large, modular protein complex remotely conserved from yeast to man that conveys regulatory signals from dna-binding transcription factors to rna polymerase ii. in saccharomyces cerevisiae, mediator is thought to be composed of 24 subunits organized in four sub-complexes, termed the head, middle, tail and cdk8 (srb8-11) modules. in this work, we have used screening and pair-wise two-hybrid approaches to investigate protein-protein contacts between budding yeast mediator subunits. t ...	2004	15477388
genomic instability is associated with lack of telomerase activation in ovarian cancer.	malignant cells are capable of an unlimited number of cell divisions, either through production of telomerase, or through the alternate lengthening of telomere (alt) mechanism. yeast cells with genomic instability have been shown to survive in the absence of telomerase by increased recombination events. we hypothesized that ovarian cancers with high microsatellite instability (msi-h) are more likely to lack telomerase activation.	2004	15477760
the human snare protein ykt6 mediates its own palmitoylation at c-terminal cysteine residues.	the yeast snare (soluble n-ethylmaleimide-sensitive fusion protein attachment protein receptor) protein ykt6 was shown to mediate palmitoylation of the fusion factor vac8 in a reaction essential for the fusion of vacuoles. here i present evidence that hykt6 (human ykt6) has self-palmitoylating activity. incubation of recombinant hykt6 with [3h]pal-coa ([3h]palmitoyl-coa) leads to covalent attachment of palmitate to c-terminal cysteine residues. the n-terminal domain of human ykt6 contains a pal- ...	2004	15479160
crystal structures of the human sumo-2 protein at 1.6 a and 1.2 a resolution: implication on the functional differences of sumo proteins.	the sumo proteins are a class of small ubiquitin-like modifiers. sumo is attached to a specific lysine side chain on the target protein via an isopeptide bond with its c-terminal glycine. there are at least four sumo proteins in humans, which are involved in protein trafficking and targeting. a truncated human sumo-2 protein that contains residues 9-93 was expressed in escherichia coli and crystallized in two different unit cells, with dimensions of a=b=75.25 a, c=29.17 a and a=b=74.96 a, c=33.2 ...	2004	15479240
yeast miro gtpase, gem1p, regulates mitochondrial morphology via a novel pathway.	cell signaling events elicit changes in mitochondrial shape and activity. however, few mitochondrial proteins that interact with signaling pathways have been identified. candidates include the conserved mitochondrial rho (miro) family of proteins, which contain two gtpase domains flanking a pair of calcium-binding ef-hand motifs. we show that gem1p (yeast miro; encoded by yal048c) is a tail-anchored outer mitochondrial membrane protein. cells lacking gem1p contain collapsed, globular, or grape-l ...	2004	15479738
electrochemical detection of xenoestrogenic and antiestrogenic compounds using a yeast two-hybrid-17-beta-estradiol system.	the goal of this study was to determine the effects of various compounds on the 17-beta-estradiol-induced dimerization of the human estrogen receptor alpha (heralpha), a nuclear transcription factor. for this purpose, we used a modified yeast two-hybrid (yth) bioassay designed to study protein-protein interactions, based on the electrochemical monitoring of heralpha dimerization and detected as beta-d-galactosidase reporter gene activity in a synthetic substrate p-aminophenyl-beta-d-galactopyran ...	2004	15713566
function of prokaryotic and eukaryotic abc proteins in lipid transport.	atp binding cassette (abc) proteins of both eukaryotic and prokaryotic origins are implicated in the transport of lipids. in humans, members of the abc protein families a, b, c, d and g are mutated in a number of lipid transport and metabolism disorders, such as tangier disease, stargardt syndrome, progressive familial intrahepatic cholestasis, pseudoxanthoma elasticum, adrenoleukodystrophy or sitosterolemia. studies employing transfection, overexpression, reconstitution, deletion and inhibition ...	2004	15749056
hiv-1 interaction with human mannose receptor (hmr) induces production of matrix metalloproteinase 2 (mmp-2) through hmr-mediated intracellular signaling in astrocytes.	astrocytes are susceptible to hiv-1 infection. we have recently demonstrated that human mannose receptor (hmr) is directly involved in cd4-independent hiv-1 infection of astrocytes. the apparent paradox between the vivid binding affinity of hiv-1 gp120 protein to hmr and the low efficiency of hmr-mediated hiv-1 infection raises the possibility that hiv-1 binding to hmr alone may negatively affect astrocyte function. in this study, we examined the relationship between hiv-1 interaction with hmr a ...	2004	15955449
antibody-directed therapy for human hepatocellular carcinoma.	the goals of our research are to develop high-affinity and high-stability antibodies and fragments thereof for targeting tumor-specific antigens in an attempt to develop new therapeutic agents for human hepatocellular carcinoma (hcc). tumor-associated antigens are excellent targets for drug and gene delivery, and offer the advantage of high cellular specificity. we have explored the use of a monoclonal antibody (mab) af-20 raised against a human hepatoma cell line (focus) as a model system. this ...	2004	15508088
[identification of proteins interacting with androgen receptor- associated coregulator 267-alpha (ara267-alpha) with the yeast two-hybrid system].	to search for proteins interacting with ara267-alpha with the yeast two-hybrid system in order to further investigate the function of ara267-alpha.	2004	15489934
ubiquilin-1 is a novel hash-1-complexing protein that regulates levels of neuronal bhlh transcription factors in human neuroblastoma cells.	the basic helix-loop-helix (bhlh) transcription factor mammalian achaete-scute homologue-1 (mash-1 in mouse and hash-1 in humans) is expressed in specific subsets of embryonic neuronal precursors of both the peripheral and central nervous systems. this gene is essential for development of olfactory and most peripheral autonomic neurons. neuro-blastoma is a pediatric malignancy derived from sympathetic nervous system precursors and hash-1 is expressed in a majority of neuroblastoma tumors and cel ...	2004	15492808
pyridoxal 5'-phosphate inactivates dna topoisomerase ib by modifying the lysine general acid.	the present results demonstrate that pyridoxal, pyridoxal 5'-phosphate (plp) and pyridoxal 5'-diphospho-5'-adenosine (plp-amp) inhibit candida guilliermondii and human dna topoisomerases i in forming an aldimine with the epsilon-amino group of an active site lysine. plp acts as a competitive inhibitor of c.guilliermondii topoisomerase i (k(i) = 40 microm) that blocks the cleavable complex formation. chemical reduction of plp-treated enzyme reveals incorporation of 1 mol of plp per mol of protein ...	2004	15494452
both cag repeats and inverted dna repeats stimulate spontaneous unequal sister-chromatid exchange in saccharomyces cerevisiae.	genomic regions containing trinucleotide repeats (tnrs) are highly unstable, as the repeated sequences exhibit a high rate of mutational change, in which they undergo either a contraction or an expansion of repeat numbers. although expansion of tnrs is associated with several human genetic diseases, the expansion mechanism is poorly understood. extensive studies in model organisms have indicated that instability of tnrs occurs by several mechanisms, including replication slippage, dna repair and ...	2004	15494455
the pathobiology of the septin gene family.	septins are an evolutionarily conserved group of gtp-binding and filament-forming proteins that belong to the large superclass of p-loop gtpases. while originally discovered in yeast as cell division cycle mutants with cytokinesis defects, they are now known to have diverse cellular roles which include polarity determination, cytoskeletal reorganization, membrane dynamics, vesicle trafficking, and exocytosis. septin proteins form homo- and hetero-oligomeric polymers which can assemble into highe ...	2004	15495264
[modification and application of recombinant yeast bioassay for measuring the ahr ligand activity].	a recombinant yeast bioassay, a yeast saccharomyces cerevisiae, in which the human ahr and arnt complex are coexpressed, is one of the methods to screen the active ahr agonist. in this study, the original agonist test was modified. the exposure time was reduced from 18 to 8 hours when experiment was under the following conditions: (1) the yeast was cultured in 0.2% glucose medium for 24 hours; (2) chemical exposure was carried in 2% galactose medium in glass tube. finally, the ahr acitivity of h ...	2004	15515957
acetylation of insulin receptor substrate-1 is permissive for tyrosine phosphorylation.	insulin receptor substrate (irs) proteins are key moderators of insulin action. their specific regulation determines downstream protein-protein interactions and confers specificity on growth factor signalling. regulatory mechanisms that have been identified include phosphorylation of irs proteins on tyrosine and serine residues and ubiquitination of lysine residues. this study investigated other potential molecular mechanisms of irs-1 regulation.	2004	15522123
cellular signaling mediated by calphoglin-induced activation of ipp and pgm.	universal protein networks conserved from bacteria to animals dictate the core functions of cells. inorganic pyrophosphatase (ipp) is an essential enzyme that plays a pivotal role in a broad spectrum of cellular biosynthetic reactions such as amino acid, nucleotide, polysaccharide, and fatty acid biosynthesis. however, the in vivo cellular regulation mechanisms of ipp and another key metabolic enzyme, phosphoglucomutase (pgm), remain unknown. this study aimed to examine the universal protein reg ...	2004	15522220
dna replication licensing in somatic and germ cells.	the dna replication (or origin) licensing system ensures precise duplication of the genome in each cell cycle and is a powerful regulator of cell proliferation in metazoa. studies in yeast, drosophila melanogaster and xenopus laevis have characterised the molecular machinery that constitutes the licensing system, but it remains to be determined how this important evolutionary conserved pathway is regulated in homo sapiens. we have investigated regulation of the origin licensing factors cdc6, cdt ...	2004	15522891
yeast two-hybrid screening for proteins that interact with alpha1-adrenergic receptors.	to find novel proteins that may bind to alpha1a-adrenergic receptor (alpha1a-ar) and investigate their interactions with the other two alpha1-ar subtypes (alpha1b-ar and alpha1d-ar) with an expectation to provide new leads for the function study of the receptors.	2004	15525470
identification of a novel shrimp protein phosphatase and its association with latency-related orf427 of white spot syndrome virus.	to characterize the role of latency-associated orf427 of white spot syndrome virus (wssv), a shrimp cdna library was constructed to screen interacting proteins of orf427. employing the yeast two-hybrid system, a novel shrimp protein phosphatase (named pps), sharing 93% homology with human protein phosphatase 1, has been identified able to bind orf427 in yeast. through co-immunoprecipitation assays, the interaction between pps and orf427 was further confirmed both in vitro and in vivo. interestin ...	2004	15527775
drosophila, an emerging model for cardiac disease.	a variety of studies that are currently underway may validate the fruit fly as an in vivo model for analyzing genes involved in cardiac function. many mutations in conserved genetic pathways have been found, including those controlling development and physiology. because homologous genes control early developmental events as well as functional components of the drosophila and vertebrate hearts, the fly is the simplest existing model system that can be used to assay genes involved in human congen ...	2004	15527959
tbp-associated factors in arabidopsis.	initiation of transcription mediated by rna polymerase ii requires a number of transcription factors among which tfiid is the major core promoter recognition factor. tfiid is composed of highly conserved factors which include the tata-binding protein (tbp) and about 14 tbp-associated factors (tafs). since tafs play important roles in transcription they have been extensively studied in organisms like yeast, drosophila and human. surprisingly, tafs have been poorly characterized in plants. with th ...	2004	15527982
uch2/uch37 is the major deubiquitinating enzyme associated with the 26s proteasome in fission yeast.	conjugation of proteins to ubiquitin plays a central role for a number of cellular processes including endocytosis, dna repair and degradation by the 26s proteasome. however, ubiquitination is reversible as a number of deubiquitinating enzymes mediate the disassembly of ubiquitin-protein conjugates. some deubiquitinating enzymes are associated with the 26s proteasome contributing to and regulating the particle's activity. here, we characterise fission yeast uch2 and ubp6, two proteasome associat ...	2004	15533439
homophilic anchorage of brain-hexokinase to mitochondria-porins revealed by specific-peptide antibody cross recognition.	in brain tumors the main source of energy is from glycolysis, which is initiated by hexokinase 1 (hk1), an enzyme bound to the mitochondrial porin. disruption of hk binding greatly affects tumor cell survival. little is known about the acceptor site of hk1. therefore, a polyclonal antibody (pab) directed to miaaqllayyftelk (mk) peptide, corresponding to the 15-amino acids of the n-terminal sequence of brain hk1 was obtained. anti mk antibody (amk-pab)bound specifically to hk as shown by elisa. t ...	2004	15562563
efficient somatic gene targeting in the lymphoid human cell line dg75.	among the different approaches used to define the function of a protein of interest, alteration and/or deletion of its encoding gene is the most direct strategy. homologous recombination between the chromosomal gene locus and an appropriately designed targeting vector results in an alteration or knockout of the gene of interest. homologous recombination is easily performed in yeast or in murine embryonic stem cells, but is cumbersome in more differentiated and diploid somatic cell lines. here we ...	2004	15563834
fad24, a mammalian homolog of noc3p, is a positive regulator in adipocyte differentiation.	adipocyte differentiation is controlled by complex actions involving gene expression and signal transduction. from metaphase to anaphase, peroxisome proliferator-activated receptor gamma, the ccaat/enhancer-binding protein family and sterol regulatory element-binding protein-1 are known to function as master regulators. however, the mechanism underlying the earliest step, which triggers the initiation of differentiation, remains unknown. in previous reports, we have isolated a number of genes, w ...	2004	15564382
jekyll and hyde in the microbial world.	fungi are nonmotile organisms that obtain carbon from compounds in their immediate surroundings. confronted with nutrient limitation, the yeast saccharomyces cerevisiae undergoes a dimorphic transition, switching from spherical cells to filaments of adherent, elongated cells that can invade the substratum. a complex web of sensing mechanisms and cooperation among signaling networks (including a mitogen-activated protein kinase cascade, cyclic adenosine monophosphate-dependent protein kinase, and ...	2004	15567850
alpha-anomeric deoxynucleotides, anoxic products of ionizing radiation, are substrates for the endonuclease iv-type ap endonucleases.	alpha-anomeric 2'-deoxynucleosides (alphadn) are one of the products formed by ionizing radiation (ir) in dna under anoxic conditions. alpha-2'-deoxyadenosine (alphada) and alpha-thymidine (alphat) are not recognized by dna glycosylases, and are likely removed by the alternative nucleotide incision repair (nir) pathway. indeed, it has been shown that alphada is a substrate for the escherichia coli nfo and human ape1 proteins. however, the repair pathway for removal of alphada and other alphadn i ...	2004	15568813
nonphosphorylated human la antigen interacts with nucleolin at nucleolar sites involved in rrna biogenesis.	la is a rna-binding protein implicated in multiple pathways related to the production of trnas, ribosomal proteins, and other components of the translational machinery (d. j. kenan and j. d. keene, nat. struct. mol. biol. 11:303-305, 2004). while most la is phosphorylated and resides in the nucleoplasm, a fraction is in the nucleolus, the site of ribosome production, although the determinants of this localization are incompletely known. in addition to its conserved n-terminal domain, human la ha ...	2004	15572691
dna replication checkpoint prevents precocious chromosome segregation by regulating spindle behavior.	the dna replication checkpoint maintains replication fork integrity and prevents chromosome segregation during replication stresses. mec1 and rad53 (human atm/atr- and chk2-like kinases, respectively) are critical effectors of this pathway in yeast. when treated with replication inhibitors, checkpoint-deficient mec1 or rad53 mutant fails to maintain replication fork integrity and proceeds to partition unreplicated chromosomes. we show that this unnatural chromosome segregation requires neither t ...	2004	15574325
hidden localization motifs: naturally occurring peroxisomal targeting signals in non-peroxisomal proteins.	can sequence segments coding for subcellular targeting or for posttranslational modifications occur in proteins that are not substrates in either of these processes? although considerable effort has been invested in achieving low false-positive prediction rates, even accurate sequence-analysis tools for the recognition of these motifs generate a small but noticeable number of protein hits that lack the appropriate biological context but cannot be rationalized as false positives.	2004	15575971
basement membrane protein and matrix metalloproteinase deregulation in engineered human oral mucosa following infection with candida albicans.	a variety of morphological changes in the basement membrane (bm) are known to occur in inflammatory diseases. modifications of the bm can be associated with significant changes in protein content. candida albicans (c. albicans) is normally a commensal organism and is a member of the natural flora of a large number of healthy individuals. however, under certain conditions, c. albicans can invade host tissues, causing inflammation and tissue damage. the aim of this study was to investigate the eff ...	2004	15579314
nutrient sensing and metabolic decisions.	cells have several sensory systems that detect energy and metabolic status and adjust flux through metabolic pathways accordingly. many of these sensors and signaling pathways are conserved from yeast to mammals. in this review, we bring together information about five different nutrient-sensing pathways (amp kinase, mtor, pas kinase, hexosamine biosynthesis and sir2), highlighting their similarities, differences and roles in disease.	2004	15581787
alteration of lithium pharmacology through manipulation of phosphoadenosine phosphate metabolism.	bisphosphate 3'-nucleotidase (bpnt1 in mammals and met22/hal2 in yeast) is one of five members of a family of signaling phosphatases united through a common tertiary structure and inhibition by subtherapeutic doses of the antibipolar drug lithium. here we report a role for 3'-nucleotidase and its substrate, 3'-phosphoadenosine 5'-phosphate (pap), in mediating the cellular effects of lithium. lithium-induced inhibition of growth in yeast cells may be overcome by dose-dependent heterologous expres ...	2004	15583009
dna replication checkpoint control of wee1 stability by vertebrate hsl7.	g2/m checkpoints prevent mitotic entry upon dna damage or replication inhibition by targeting the cdc2 regulators cdc25 and wee1. although wee1 protein stability is regulated by dna-responsive checkpoints, the vertebrate pathways controlling wee1 degradation have not been elucidated. in budding yeast, stability of the wee1 homologue, swe1, is controlled by a regulatory module consisting of the proteins hsl1 and hsl7 (histone synthetic lethal 1 and 7), which are targeted by the morphogenesis chec ...	2004	15583029
quantification and assessment of viability of cryptococcus neoformans by lightcycler amplification of capsule gene mrna.	cryptococcus neoformans is an opportunistic fungal pathogen. it infects the central nervous system causing meningitis, which is fatal if untreated, especially in aids and immunosuppressed patients. in this study a method of quantification and assessment of viability of c. neoformans by lightcycler rt-pcr amplification of the capsule gene mrna is established. the sequence of primers and probes were derived from c. neoformans capsular cap10 gene mrna (genbank accession number af144574), and were s ...	2004	15585498
basic helix-loop-helix transcription factor tcfl5 interacts with the calmegin gene promoter in mouse spermatogenesis.	in mouse spermatogenesis, differentiating germ line cells initiate expression of specific genes at subsequent developmental steps. the calmegin (clgn) gene is first expressed in meiotic prophase, in primary spermatocytes, and encodes a protein that acts as a chaperone. to identify testis-specific transcription factors that control expression of the clgn gene in spermatogenesis, we performed a yeast one-hybrid screening with a clgn promoter sequence as bait dna. this screening resulted in the ide ...	2004	15585666
protein partners of c/ebpepsilon.	ccaat-enhancer binding protein-epsilon (c/ebpepsilon) is a nuclear transcription factor implicated in the regulation of terminal myeloid differentiation. using a yeast two-hybrid screen, potential interaction partners of c/ebpepsilon involved in myeloid development were identified. c/ebpepsilon was found to associate with other c/ebp family members, including c/ebpepsilon and chop as well as other proteins that are known to contain a leucine-zipper protein interaction motif including creb2, ldoc ...	2004	15588942
periplakin interferes with g protein activation by the melanin-concentrating hormone receptor-1 by binding to the proximal segment of the receptor c-terminal tail.	in mice genetic ablation of expression of either melanin-concentrating hormone or the melanin-concentrating hormone-1 receptor results in alterations in energy metabolism and a lean phenotype. there is thus great interest in the function and regulation of this receptor. using the yeast two-hybrid system we identified an interaction of the actin- and intermediate filament-binding protein periplakin with the intracellular c-terminal tail of the melanin-concentrating hormone-1 receptor. direct asso ...	2004	15590649
kre5 gene null mutant strains of candida albicans are avirulent and have altered cell wall composition and hypha formation properties.	the udp-glucose:glycoprotein glucosyltransferase (uggt) is an endoplasmic reticulum sensor for quality control of glycoprotein folding. saccharomyces cerevisiae is the only eukaryotic organism so far described lacking uggt-mediated transient reglucosylation of n-linked oligosaccharides. the only gene in s. cerevisiae with similarity to those encoding uggts is kre5. s. cerevisiae kre5 deletion strains show severely reduced levels of cell wall beta-1,6-glucan polymer, aberrant morphology, and extr ...	2004	15590817
src phosphorylates ezrin at tyrosine 477 and induces a phosphospecific association between ezrin and a kelch-repeat protein family member.	ezrin, a linker between plasma membrane and actin cytoskeleton possesses morphogenic properties and can promote dissemination of tumor cells. ezrin is phosphorylated on tyrosine, but a detailed picture of the signaling pathways involved in this modification is lacking. the transforming tyrosine kinase src has various cytoskeletal substrates and is involved in regulation of cellular adhesion. we studied the role of src in tyrosine phosphorylation of ezrin in adherent cells. we show that ezrin is ...	2004	15623525
k-cone analysis: determining all candidate values for kinetic parameters on a network scale.	the absence of comprehensive measured kinetic values and the observed inconsistency in the available in vitro kinetic data has hindered the formulation of network-scale kinetic models of biochemical reaction networks. to meet this challenge we present an approach to construct a convex space, termed the k-cone, which contains all the allowable numerical values of the kinetic constants in large-scale biochemical networks. the definition of the k-cone relies on the incorporation of in vivo concentr ...	2004	15626710
composition and functional specificity of swi2/snf2 class chromatin remodeling complexes.	by regulating the structure of chromatin, atp-dependent chromatin remodeling complexes (remodelers) perform critical functions in the maintenance, transmission and expression of the eukaryotic genome. although all known chromatin-remodeling complexes contain an atpase as a central motor subunit, a number of distinct classes have been recognized. recent studies have emphasized a more extensive functional diversification among closely related chromatin remodeling complexes than previously anticipa ...	2004	15627498
induction of apoptosis in human leukemia cells by naturally fermented sugar cane vinegar (kibizu) of amami ohshima island.	naturally fermented vinegar such as kibizu (sugar cane vinegar in amami ohshima, japan), kurozu (black rice vinegar in kagoshima, japan), kouzu (black rice vinegar in china) and red wine vinegar in italy had potent radical-scavenging activity analyzed by dpph method. for the elucidation of food factor for cancer prevention contained in naturally fermented vinegar, the induction of apoptosis in human leukemia cell hl-60 was investigated with sugar cane vinegar kibizu. fraction eluted by 40% metha ...	2004	15630260
recruitment of the ino80 complex by h2a phosphorylation links atp-dependent chromatin remodeling with dna double-strand break repair.	the budding yeast ino80 complex is a conserved atp-dependent nucleosome remodeler containing actin-related proteins arp5 and arp8. strains lacking ino80, arp5, or arp8 have defects in transcription. here we show that these mutants are hypersensitive to dna damaging agents and to double-strand breaks (dsbs) induced by the ho endonuclease. the checkpoint response and most transcriptional modulation associated with induction of dna damage are unaffected by these mutations. using chromatin immunopre ...	2004	15607975
ttk/hmps1 participates in the regulation of dna damage checkpoint response by phosphorylating chk2 on threonine 68.	chk2/hcds1 plays important roles in the dna damage-induced cell cycle checkpoint by phosphorylating several important targets, such as cdc25 and p53. to obtain a better understanding of the chk2 signaling pathway, we have carried out a yeast two-hybrid screen to search for potential chk2-interacting proteins. here, we report the identification of the mitotic checkpoint kinase, ttk/hmps1, as a novel chk2-interacting protein. ttk/hmps1 directly phosphorylates chk2 on thr-68 in vitro. expression of ...	2004	15618221
novel association of vav2 and nek3 modulates signaling through the human prolactin receptor.	prolactin (prl) receptor activation contributes to the progression and motility of human breast cancer. this event activates multimeric signaling pathways, including the activation of the vav family of guanine nucleotide exchange factors. to detect novel proteins interacting with vav, yeast two-hybrid analysis was performed and demonstrated an interaction between the serine/threonine nima (never in mitosis a)-related family kinase p56nek3 and vav1. the prl-dependent interaction of nek3 with vav1 ...	2004	15618286
physical and genetic interactions link the yeast protein zds1p with mrna nuclear export.	eukaryotic gene expression requires the export of mrna from the nucleus to the cytoplasm. the dead box protein dbp5p is an essential export factor conserved from yeast to man. a fraction of dbp5p forms a complex with nucleoporins of the cytoplasmic filaments of the nuclear pore complex. gfd1p was identified originally as a multicopy suppressor of the rat8-2 ts allele of dbp5. here we reported that dbp5p and gfd1p interact with zds1p, a protein previously identified as a multicopy suppressor in s ...	2004	15619606
hailey-hailey disease and calcium: lessons from yeast.		2004	15610534
inhibition of simian virus 40 large tumor antigen expression in human fetal glial cells by an antisense oligodeoxynucleotide delivered by the jc virus-like particle.	human jc virus (jcv) is a neurotropic virus, and the etiological agent of progressive multifocal leukoencephalopathy (pml), a fatal neurological disease. because of its natural infection tropism, it is possible to use the jcv capsid as a gene-transducing vector for therapeutic purposes in neurological disorders. in the current study, a recombinant jcv virus-like particle (vlp) was generated and purified from yeast. vlp was able to accommodate and protect dna molecules of up to approximately 2000 ...	2004	15610608
atp hydrolysis by orc catalyzes reiterative mcm2-7 assembly at a defined origin of replication.	the origin recognition complex (orc) is a six-subunit, atp-regulated, dna binding protein that is required for the formation of the prereplicative complex (pre-rc), an essential replication intermediate formed at each origin of dna replication. in this study, we investigate the mechanism of orc function during pre-rc formation and how atp influences this event. we demonstrate that atp hydrolysis by orc requires the coordinate function of the orc1 and orc4 subunits. mutations that eliminate orc a ...	2004	15610739
ubc9 and protein inhibitor of activated stat 1 activate chicken ovalbumin upstream promoter-transcription factor i-mediated human cyp11b2 gene transcription.	aldosterone synthase (cyp11b2) is involved in the final steps of aldosterone biosynthesis and expressed exclusively in the adrenal zona glomerulosa cells. using an electrophoretic mobility shift assay, we demonstrate that coup-tfi binds to the -129/-114 element (ad5) of human cyp11b2 promoter. transient transfection in h295r adrenal cells demonstrated that coup-tfi enhanced cyp11b2 reporter activity. however, the reporter construct with mutated ad5 sequences showed reduced basal and coup-tfi-enh ...	2004	15611122
examination of the roles of sgs1 and srs2 helicases in the enforcement of recombination fidelity in saccharomyces cerevisiae.	mutation in sgs1, which encodes the yeast homolog of the human bloom helicase, or in mismatch repair (mmr) genes confers defects in the suppression of mitotic recombination between similar but nonidentical (homeologous) sequences. mutational analysis of sgs1 suggests that the helicase activity is required for the suppression of both homologous and homeologous recombination and that the c-terminal 200 amino acids may be required specifically for the suppression of homeologous recombination. to cl ...	2004	15611162
3d models of yeast rnase p/mrp proteins rpp1p and pop3p.	sensitive profile searches and fold recognition were used to predict the structures of two yeast rnase p/mrp proteins. rpp1p, which is one of the subunits common to eukaryotes and archaea, is predicted to adopt the seven-stranded tim-barrel fold found in php phosphoesterases. pop3p, initially thought to be one of the rnase p/mrp subunits unique to yeast, has been assigned the l7ae/l30e fold. this rna-binding fold is also present in human rnase p subunit rpp38, raising the possibility that pop3p ...	2004	15613537
how heterologously expressed escherichia coli genes contribute to understanding dna repair processes in saccharomyces cerevisiae.	dna-damaging agents constantly challenge cellular dna; and efficient dna repair is therefore essential to maintain genome stability and cell viability. several dna repair mechanisms have evolved and these have been shown to be highly conserved from bacteria to man. dna repair studies were originally initiated in very simple organisms such as escherichia coli and saccharomyces cerevisiae, bacteria being the best understood organism to date. as a consequence, bacterial dna repair genes encoding pr ...	2004	15614491
the forkhead-associated domain protein cep170 interacts with polo-like kinase 1 and serves as a marker for mature centrioles.	we report the characterization of cep170, a forkhead-associated (fha) domain protein of previously unknown function. cep170 was identified in a yeast two-hybrid screen for interactors of polo-like kinase 1 (plk1). in human cells, cep170 is constantly expressed throughout the cell cycle but phosphorylated during mitosis. it interacts with plk1 in vivo and can be phosphorylated by plk1 in vitro, suggesting that it is a physiological substrate of this kinase. both overexpression and small interferi ...	2004	15616186
molecular characterization of the tobacco set domain protein ntset1 unravels its role in histone methylation, chromatin binding, and segregation.	plants contain a great number of genes encoding a distinctive class of set domain proteins which harbor a plant-specific n-terminal part together with a c-terminal part showing highest sequence similarity to the catalytic domain of the yeast clr4, the human suv39h1 and g9a histone-methyltransferases (hmtases). here we show that ntset1, a representative member of this class from tobacco, methylated both k9 and k27 of histone h3 in vitro. ectopic expression of ntset1, by an inducible promoter, inc ...	2004	15546353
an smc-domain protein in fission yeast links telomeres to the meiotic centrosome.	abnormal centrosomal structures similar to those occurring in human cancers are induced in fission yeast by overexpression of the pericentrin homolog pcp1p. analysis of abnormal pcp1p-containing structures with quantitative mass spectrometry and isotope-coded affinity tags identified a coiled-coil, structural maintenance of chromosomes (smc) domain protein. this protein, termed ccq1p (coiled-coil protein quantitatively enriched), localizes with taz1p to telomeres in normal vegetative cells. fluo ...	2004	15546621
the ring domain of mdm2 mediates histone ubiquitylation and transcriptional repression.	histone modifications play a pivotal role in regulating transcription and other chromatin-associated processes. in yeast, histone h2b monoubiquitylation affects gene silencing. however, mammalian histone ubiquitylation remains poorly understood. we report that the mdm2 oncoprotein, a ring domain e3 ubiquitin ligase known to ubiquitylate the p53 tumor suppressor protein, can interact directly with histones and promote in vitro monoubiquitylation of histones h2a and h2b. moreover, mdm2 induces h2b ...	2004	15546622
cp-31398, a putative p53-stabilizing molecule tested in mammalian cells and in yeast for its effects on p53 transcriptional activity.	cp-31398 is a small molecule that has been reported to stabilize the dna-binding core domain of the human tumor suppressor protein p53 in vitro. the compound was also reported to function as a potential anti-cancer drug by rescuing the dna-binding activity and, consequently, the transcription activation function of mutant p53 protein in mammalian tissue culture cells and in mice.	2004	15548325
chromatographic speciation of anionic and neutral selenium compounds in se-accumulating brassica juncea (indian mustard) and in selenized yeast.	selenium-accumulating plants such as brassica juncea (indian mustard) concentrate the element in plant shoots and roots. such behavior may provide a cost-effective technology to clean up contaminated soils and waters that pose major environmental and human health problems (phytoremediation). such ability to transform selenium into bioactive compounds has important implications for human nutrition and health. element selective characterization of b. juncea grown in the presence of inorganic selen ...	2004	15553157
[study on binding of hbeag to cd81].	to investigate the interaction between hbeag and cd81.	2004	15555435
ability of human cdc25b phosphatase splice variants to replace the function of the fission yeast cdc25 cell cycle regulator.	cdc25 phosphatases are essential and evolutionary-conserved actors of the eukaryotic cell cycle control. to examine and compare the properties of three splicing variants of human cdc25b, recombinant fission yeast strains expressing the human proteins in place of the endogenous cdc25 were generated and characterized. we report, that the three cdc25b variants: (i) efficiently replace the yeast counterpart in vegetative growth, (ii) partly restore the gamma and uv radiation dna damage-activated che ...	2004	15556082
energy restriction and aging.	the focus of this review is on current research involving long-term calorie restriction and the resulting changes observed in possible biomarkers of aging. special emphasis will be given to the basic and clinical science studies which are currently investigating the effects of controlled, high-quality energy-restricted diets on both biomarkers of longevity and on the development of chronic diseases related to age and obesity in humans.	2004	15534428
structure of human pot1 bound to telomeric single-stranded dna provides a model for chromosome end-protection.	the pot1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. it is essential for chromosome end-protection in the fission yeast schizosaccharomyces pombe, and it is involved in regulation of telomere length in human cells. here, we report the crystal structure at a resolution of 1.73 a of the n-terminal half of human pot1 (hpot1) protein bound to a telomeric single-stranded dna (ssdna) decamer, ttagggttag, the minimum tight-bin ...	2004	15558049
studies of cox16, cox19, and pet191 in human cytochrome-c oxidase deficiency.	cytochrome-c oxidase (cox) is the terminal enzyme of the mitochondrial electron transport chain, and cox deficiency is a common cause of mitochondrial diseases. cytochrome-c oxidase is composed of 13 subunits, of which 3 are encoded by mitochondrial dna and 10 by nuclear dna. mutations have been identified in each of the 3 mitochondrial dna genes but in none of the nuclear dna genes. however, cox deficiency has been attributed to mutations in several nuclear dna-encoded ancillary proteins needed ...	2004	15596615
detection of genistein as an estrogenic contaminant of river water in osaka.	the estrogenic activity in water at various localities on lake biwa-yodo river, a representative watershed in japan, was measured using a recombinant yeast that expresses the human estrogen receptor. the yeast bioassay revealed that the activities of 13 water samples had an average value of 14 pmol/l (3.8 ng/l) (17beta-estradiol equivalent) with a very wide range from 0 to 72 pmol/l (0-19.6 ng/ l), and two of the samples had prominent levels of activity (72 pmol/l (19.6 ng/l) and 56 pmol/l (15.2 ...	2004	15597900
man1, an integral protein of the inner nuclear membrane, binds smad2 and smad3 and antagonizes transforming growth factor-beta signaling.	man1 (also known as lemd3) is an integral protein of the inner nuclear membrane. recently, mutations in man1 have been shown to result in osteopoikilosis, buschke-ollendorff syndrome and melorheostosis. we show that the nucleoplasmic, c-terminal domain of human man1 binds to smad2 and smad3 and antagonizes signaling by transforming growth factor-beta (tgf-beta). in a yeast two-hybrid screen using the c-terminal domain of man1 as bait, eight positive clones were obtained that encoded smad3. in di ...	2004	15601644
a comprehensive study on the immunological reactivity of the hsp90 molecular chaperone.	periodontitis is a chronic infectious disease, porphyromonas gingivalis being the most implicated pathogen. in the present study, we investigated the role of p. gingivalis htpg (pghtpg), a bacterial ortholog of mammalian hsp90, in the growth of p. gingivalis and also assessed the immunological cross-reactivity of the members of the hsp90 family. antiserum against rat liver hsp90 potently reacted with yeast hsp90, called hsc82, and also weakly with human hsp90 (hhsp90) and human mitochondrial par ...	2004	15632312
identification and functional analysis of two rare allelic variants of the thiopurine s-methyltransferase gene, tpmt16 and tpmt19.	human thiopurine s-methyltransferase (tpmt) catalyses the s-methylation of thiopurine drugs. tpmt is genetically polymorphic and is associated with large interindividual variations in thiopurine drug toxicity and therapeutic efficacy. during routine genotyping of patients with crohn's disease, one novel missense mutation, 365a>c (tpmt19, lys(122)thr), and a recently described missense mutation, 488g>a (tpmt16, arg(163)his), were identified in a caucasian and a moroccan patient, respectively. u ...	2004	15652243
detection of mitochondrial dna depletion in living human cells using picogreen staining.	human mitochondria dna (mtdna) is arranged within the mitochondria into discrete dna-protein complexes, termed nucleoids. the size of the human mitochondrial genome is less than that of yeast and is more difficult to visualise by fluorescent dna stains such as dapi and hoescht. we have developed a simple yet effective method to visualise mtdna in situ within living cells using the fluorescent stain picogreen. quantitative analysis shows that picogreen can be used to estimate the degree of mtdna ...	2004	15652355
expression and characterization of human tumor necrosis factor-related apoptosis inducing ligand in methylotrophic yeast pichia pastoris.	recombinant human trail was successfully expressed in a secreted form in methyltrophic yeast pichia pastoris induced by methanol. the expressive product was immunoreactive to trail antibody. the addition of the expressive product into cultured human lung cancer cell a549 showed moderate cell death, typical morphological characterization of apoptotic bodies, and dna fragmentation. this result provided a new method to produce recombinant human trail as a cancer therapeutic drug.	2004	15646010
functional xpb/rad25 redundancy in arabidopsis genome: characterization of atxpb2 and expression analysis.	the xeroderma pigmentosum complementation group b (xpb) protein is involved in both dna repair and transcription in human cells. it is a component of the transcription factor iih (tfiih) and is responsible for dna helicase activity during nucleotide (nt) excision repair (ner). its high evolutionary conservation has allowed identification of homologous proteins in different organisms, including plants. in contrast to other organisms, arabidopsis thaliana harbors a duplication of the xpb orthologu ...	2004	15656976
anti-cancer and pro-apoptotic effects of an herbal medicine and saccharomyces cerevisiae product (ckbm) on human hepatocellular carcinoma hepg2 cells in vitro and in vivo.	hepatocellular carcinoma is a major health problem worldwide. different treatment strategies have been developed to cope with this problem. herbal medicine is now widely studied in both eastern and western countries. in this study, we used both in vitro and in vivo model to illustrate the anti-tumor effect of a product, ckbm, consisting of herbal medicine and specially processed saccharomyces cerevisiae. dose-dependent anti-proliferation effect was observed on in vitro growth of human hepatoma h ...	2004	15658608
eif2 and the control of cell physiology.	eukaryotic initiation factor eif2 and its 'exchange factor' eif2b play a key role in the regulation of protein synthesis in eukaryotes from yeast to mammals. phosphorylation of eif2 inhibits eif2b and thus translation initiation. four eif2 kinases are now known in mammalian cells and these are activated in response to specific stress conditions. while phosphorylation of eif2 serves to impair general protein synthesis, it causes upregulation of the translation of certain specific mrnas that encod ...	2004	15659334
design and characterization of a new class of inhibitors of ras activation.	the ras proteins, which regulate intracellular signaling by a cyclic process involving interconversion between active gtp-bound and inactive gdp-bound states, play an essential role in controlling the activity of several crucial signaling pathways regulating normal cellular proliferation. mutational activation of ras genes can induce cancer in humans and other mammals. about 30% of human tumors contain an altered oncogenic ras; therefore, inhibitors of ras activation are potentially antineoplast ...	2004	15659780
alternatively spliced protein variants as potential therapeutic targets for male infertility and contraception.	mammalian sperm were previously shown to express the pp1gamma2 isoform of protein phosphatase 1 (pp1) as well as its regulatory proteins inhibitor 2 and glycogen synthase kinase 3. furthermore, the development of sperm motility during transit through the epididymis correlates with changes in pp1 activity. thus, since pp1 cellular activity is determined by the partners it binds, we embarked on a study aimed at defining the specific interactomes of pp1gamma1 and pp1gamma2 (the two known alternativ ...	2004	15659832
decapping reaction of mrna requires dcp1 in fission yeast: its characterization in different species from yeast to human.	cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mrna decay pathways, and the protein complex consisting of dcp1 and dcp2 has been identified as the species responsible for the decapping reaction in saccharomyces cerevisiae and human. although in vitro studies indicate that dcp2 is catalytically an active component, the role of dcp1 in the decapping reaction remains to be explored in organisms other than budding yeast. to elucidate the dcp1-dependent decap ...	2004	15671491
efficient secretion of human lysozyme from the yeast, kluyveromyces lactis.	efficient secretion of human lysozyme from the yeast, kluyveromyces lactis, was achieved by using more stable vectors in the order of s11 replication origin-containing episomal vector < full-length k. lactis plasmid pkd1-containing vector < centromeric vector < chromosome-integrated vectors. cells containing a pgk (phosphoglycerate kinase) promoter-driven integration vector grown in non-selective rich medium achieved the highest level of secretion, approximately 100 microg lysozyme secretion ml( ...	2004	15672218
in vitro bioactivity of 17alpha-estradiol.	a miniaturised short-term in vitro assay based on the activation of the human estrogen receptor alpha and genetically modified yeast (saccharomyces cerevisiae) cells was performed to explore the capacity of this system to monitor the bioactivity of estrogenic compounds, particularly 17alpha- and 17beta-estradiol. together with the human estrogen receptor (her)-alpha plasmid, the reporter plasmid containing a yeast-optimised version of the green fluorescent protein (yegfp) linked to three repeats ...	2004	15698550
human kanadaptin and kidney anion exchanger 1 (kae1) do not interact in transfected hek 293 cells.	kanadaptin (kidney anion exchanger adaptor protein) is a widely expressed protein, shown previously to interact with the cytosolic domain of mouse cl-/hco3- anion exchanger 1 (kae1) but not erythroid ae1 (eae1) by a yeast-two hybrid assay. kanadaptin was co-localized with kae1 in intracellular membranes but not at the plasma membrane in alpha-intercalated cells of rabbit kidney. it was suggested that kanadaptin is an adaptor protein or chaperone involved in targeting kae1 to the plasma membrane. ...	2004	15764369
recurrence time statistics: versatile tools for genomic dna sequence analysis.	with the completion of the human and a few model organisms' genomes, and the genomes of many other organisms waiting to be sequenced, it has become increasingly important to develop faster computational tools which are capable of easily identifying the structures and extracting features from dna sequences. one of the more important structures in a dna sequence is repeat-related. often they have to be masked before protein coding regions along a dna sequence are to be identified or redundant expr ...	2004	16447998
an in vitro assessment of the effects of broad-spectrum antibiotics on the human gut microflora and concomitant isolation of a lactobacillus plantarum with anti-candida activities.	chemostat culture was used to determine the effects of the antimicrobial agents tetracycline and nystatin on predominant components of the human gut microflora. their addition to mixed culture systems caused a non-specific, and variable, decrease in microbial populations, although tetracycline allowed an increase in numbers of yeasts. both had a profound inhibitory effect upon populations seen as important for gut health (bifidobacteria, lactobacilli). however, a tetracycline resistant lactobaci ...	2004	16701514
[chip on chip for transcriptional regulatory network analysis].		2004	15669244
[interaction of connexin 26 with the c-terminal of neuroendocrine specific protein].	to screen and identify the interactive proteins with connexin 26 (cx26) by the yeast two hybrid technique.	2004	16134590
analysis of estrogen-like compounds in the environment by high performance liquid chromatography bioassay.	to identify chemicals with endocrine-disrupting activity easily, we developed a new bioassay system, consisting of bioassay using genetically modified yeast expressing human estrogen receptor and high performance liquid chromatography (hplc), in which advantages of instrumental analysis and bioassay are combined. the peaks in the mixture of these estrogen-like compounds analyzed using an hplc bioassay were similar to those obtained by analysis using an hplc-uv detector. underground water and sea ...	2004	16233618
fhl2, ubc9, and pias1 are novel estrogen receptor alpha-interacting proteins.	estrogen plays important roles in the pathophysiology of atherosclerosis and cardiovascular diseases mediated by estrogen receptor alpha (eralpha). to elucidate the molecular mechanisms, we screened eralpha-interacting proteins from a human heart cdna library using a yeast two-hybrid system, and identified the four and a half of lim-only protein 2 (fhl2). fhl2 interacted with eralpha in the presence of 17beta-estradiol, but not of tamoxifen or raloxifene in yeast. fhl2 mainly interacted with n-t ...	2004	15666801
identification of calcium-modulating cyclophilin ligand (caml) as transducer of angiotensin ii-mediated nuclear factor of activated t cells (nfat) activation.	angiotensin ii (ang ii) plays a central role in cardiovascular physiology and disease. ang ii type i receptor (at1) is thought to mediate most actions of ang ii. a novel at1 receptor intracellular partner called at1 receptor-associated protein (atrap) was identified, but its exact function has not been elucidated. a yeast two-hybrid screen using atrap as bait identified calcium-modulating cyclophilin ligand (caml) as an atrap partner. yeast two-hybrid and coimmunoprecipitation analysis demonstra ...	2005	15668245
a novel splice variant of human xrn2 gene is mainly expressed in blood leukocyte.	the xrn2 gene (xrn2a) is the human homologue of the saccharomyces cerevisiae rat1 gene, which encodes a nuclear 5'-->3' exoribonuclease, and is essential for rna metabolism and cell viability. xrn2p/rat1p, product of xrn2/rat1 gene, functions in the mrna degradation and processing of rrnas and small nucleolar rnas (snornas) in the nucleus. here we describe the cloning and characterization of a novel splice variant of the human xrn2 gene (xrn2b). the 3271-bp cdna encodes a putative protein with 9 ...	2005	16147866
selecting and screening recombinant antibody libraries.	during the past decade several display methods and other library screening techniques have been developed for isolating monoclonal antibodies (mabs) from large collections of recombinant antibody fragments. these technologies are now widely exploited to build human antibodies with high affinity and specificity. clever antibody library designs and selection concepts are now able to identify mab leads with virtually any specificity. innovative strategies enable directed evolution of binding sites ...	2005	16151404
complementation analyses suggest species-specific functions of the snf5 homology domain.	inactivation on both alleles of the hsnf5/ini1 tumor suppressor gene which encodes a subunit of the human swi/snf chromatin remodelling complex occurs in most malignant rhabdoid tumors. no paralog of hsnf5/ini1 is identified in the human genome. in contrast, it has two homologs in the yeast saccharomyces cerevisiae, snf5 and sfh1 which encode core components of the yswi/snf and rsc complexes, respectively. the homology mainly concerns an approximately 200 amino acid region termed the snf5 homolo ...	2005	16154112
synthesis and antifungal activity of oxygenated cholesterol derivatives.	a series of oxygenated cholesterol derivatives were prepared from new synthetic methods and evaluated for their in vitro antimicrobial properties against human pathogens. the activity was highly dependent on the structure of the different compounds involved. the best results were obtained with hydroxy ketones 2, 4 and 5 and diketone 7 exhibiting activities against s. cerevisiae (atcc 28383) and candida albicans (cip 1663-86). for example, compound 2 exhibited high activities against c. albicans ...	2005	16139854
the 41-amino-acid c-terminal region of the hepatitis e virus orf3 protein interacts with bikunin, a kunitz-type serine protease inhibitor.	hepatitis e virus (hev), a human plus-stranded rna virus, contains three open reading frames (orf). of these, orf1 encodes the viral nonstructural polyprotein, orf2 encodes the major capsid protein, and orf3 codes for a phosphoprotein of undefined function. recently, using the yeast two-hybrid system to screen a human cdna liver library, we have isolated and characterized ambp (alpha1-microglobulin/bikunin precursor), which specifically interacts with the orf3 protein of hev. the orf3 protein ex ...	2005	16140784
immuno-regulatory effects of ckbm on the activities of mitogen-activated protein kinases and the release of cytokines in thp-1 monocytic cells.	ckbm is an herbal formula composed of five chinese medicinal herbs (panax ginseng, schisandra chinensis, fructus crataegi, ziziphus jujube and glycine max) supplemented with processed saccharomyces cerevisiae. previous studies have demonstrated that ckbm is capable of triggering the release of il-6 and tnfalpha from human peripheral blood mononuclear cells, and its anti-tumorigenic activity has been demonstrated in nude mice with gastric cancer. in this report, we utilized the thp-1 monocytic ce ...	2005	16141532
analysis of p53 status in human cell lines using a functional assay in yeast: detection of new non-sense p53 mutation in codon 124.	p53 tumor suppressor is a sequence-specific dna-binding protein that controls the expression of many genes in response to diverse stress stimuli. p53 gene is often mutated in human cancer and in cancer cell lines. several methods are available for identification of p53 mutations, including functional analysis of separated alleles in yeast (fasay). fasay distinguishes yeast colonies expressing functional p53 protein from colonies producing a dysfunctional p53 protein simply on the basis of color. ...	2005	16142349
isolation of lignans and biological activity studies of ephedra viridis.	phytochemical investigation of ephedra viridis (whole plant) led to the isolation of four lignans including (+)-9-acetoxyisolariciresinol, which is a new lignan, lariciresinol, 9-acetoxylariciresinol and isolariciresinol. all the isolates were tested for their antioxidant activity and cytotoxicity against a panel of solid tumor and human leukemia cells. they were also screened for estrogenic activity using a recombinant yeast estrogen screening (yes) assay. most of the lignans exhibited moderate ...	2005	16142651
pias1 interaction and sumoylation of metabotropic glutamate receptor 8.	group iii presynaptic metabotropic glutamate receptors (mglurs) play a central role in regulating presynaptic activity through g-protein effects on ion channels and signal transducing enzymes. like all class c g-protein-coupled receptors, mglur8 has an extended intracellular c-terminal domain (ctd) presumed to allow for modulation of downstream signaling. in a yeast two-hybrid screen of an adult rat brain cdna library with the ctds of mglur8a and 8b (mglur8-c) as baits, we identified sumo1 and f ...	2005	16144832
expression of escherichia coli heat-labile enterotoxin b subunit (ltb) in saccharomyces cerevisiae.	heat-labile enterotoxin b subunit (ltb) of enterotoxigenic escherichia coli (etec) is both a strong mucosal adjuvant and immunogen. it is a subunit vaccine candidate to be used against etec-induced diarrhea. it has already been expressed in several bacterial and plant systems. in order to construct yeast expressing vector for the ltb protein, the eltb gene encoding ltb was amplified from a human origin enterotoxigenic e. coli dna by pcr. the expression plasmid pltb83 was constructed by inserting ...	2005	16145550