Publications

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distribution and antimicrobial susceptibility of vibrio species associated with zooplankton in coastal area of korea.vibrio parahaemolyticus and v. vulnificus are the most common pathogens causing seafood-borne illnesses in korea. this study determines the abundance and antimicrobial resistance of pathogenic vibrio species in seawater and zooplankton samples from the geoje island coast in korea, which is an important area for coastal fisheries, the fishing industry, and tourism. the two vibrio species were detected more in mesozooplankton samples than in seawater samples. v. parahaemolyticus isolates showed gr ...201728781188
function of two ficolin-like proteins in innate immune defense of the oriental river prawn, macrobrachium nipponense.ficolins have crucial functions in recognizing and eliminating pathogens in innate immunity. in this study, we identified two ficolin-like genes from the oriental river prawn, macrobrachium nipponense. these genes were designated as mnfico1 and mnfico2. mnfico1 cdna has 1600 bp, whereas mnfico2 has 1486 bp. in addition to a coiled-coil region or a low complexity region, the two ficolins both contained a signal peptide and a fibrinogen-related domain. qrt-pcr results showed that the highest expre ...201728764985
nmr-based metabolomics reveals the metabolite profiles of vibrio parahaemolyticus under ferric iron stimulation.vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. in our previous research, the protein expression of v. parahaemolyticus in fe(3+) medium was determined using isobaric tags for relative and absolute quantitation (itraq). here, nuclear magnetic resonance (nmr) was used to detect changes in the v. parahaemolyticus metabolome. nmr spectra were obtained using methanol-water extracts of intracellular metabolites ...201728752295
identification of an mitf gene and its polymorphisms associated with the vibrio resistance trait in the clam meretrix petechialis.microphthalmia-associated transcription factor (mitf) regulates the transcription of its target genes by binding to their promoters. in this study, an mitf gene, mpmitf was identified in the clam meretrix petechialis. the full-length cdna of mpmitf is 3564 bp with an orf of 1365 bp. the deduced amino acid sequence consists of a conserved functional structure of bhlh-lz, which could bind with e-box. the mrna and protein expression levels of mpmitf were significantly up-regulated 6 h post-vibrio i ...201728734968
characterizing the adherence profiles of virulent vibrio parahaemolyticus isolates.the human pathogen vibrio parahaemolyticus is a leading cause of seafood-borne illness in the usa, and infections with v. parahaemolyticus typically result from eating raw or undercooked oysters. v. parahaemolyticus has been shown to be highly resistant to oyster depuration, suggesting that the bacterium possesses specific mechanisms or factors for colonizing oysters and persisting during depuration. in this study, we characterized eight different v. parahaemolyticus strains for differences in r ...201728717834
antibacterial activity of food-grade chitosan against vibrio parahaemolyticus biofilms.biofilm is a community composed of microbes and the extracellular polymeric substances. this special architecture poses a significant public health risk as it increases the fitness of bacteria in harsh conditions and renders bacterial resistance to antimicrobial agents and cleaning. in this study, we investigated the inhibition and eradication effects of chitosan on the biofilm of vibrio parahaemolyticus, an important food-borne pathogen. the crystal violet staining, [2, 3-bis (2-methoxy-4-nitro ...201728710011
molecular characterization and expression analyses of toll like receptor-5 induced by vibrio parahaemolyticus antigens in pacific red snapper.toll-like receptor 5 (tlr5) is a member of tlrs family responsible for the bacterial flagellin recognition in vertebrates. herein, the tlr5m gene structure of pacific red snapper (lutjanus peru) was characterized. the full-length cdna of lptlr5m comprises an open reading frame (orf) of 2715 bp, encoding a polypeptide of 904 amino acids including 9 lrrs (residues 119-562) and one lrr-ct domain (residues 593-646) at the extracellular region, and a tir domain (residues 710-904) in the cytoplasmic r ...201728698126
parallel evolution of two clades of a major atlantic endemic vibrio parahaemolyticus pathogen lineage by independent acquisition of related pathogenicity islands.shellfish-transmitted vibrio parahaemolyticus infections have recently increased from locations with historically low disease incidence, such as the northeast united states (us). this change coincided with a bacterial population shift towards human pathogenic variants occurring in part through the introduction of several pacific native lineages (st36, st43 and st636) to near-shore areas off the atlantic coast of the northeast us. concomitantly, st631 emerged as a major endemic pathogen. phylogen ...201728687650
characterization of clinical vibrio parahaemolyticus strains in zhoushan, china, from 2013 to 2014.vibrio parahaemolyticus is recognized as major cause of foodborne illness of global public health concern. this study collected 107 strains of v. parahaemolyticus during active surveillance of diarrheal diseases in hospitals in zhoushan during 2013 to 2014 and investigated their serotypes, virulence genes (tdh, trh, and orf8), antimicrobial resistance, and genotypes. the dominant serotypes of the 107 clinical strains were o3:k6, o4:k8, and o4:kut with 87.9% and 3.7% of the strains carrying the v ...201728678810
prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from short mackerels (rastrelliger brachysoma) in malaysia.numerous prevalence studies and outbreaks of vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. information on the quantitative detection of v. parahaemolyticus in finfish species is limited. in this study, short mackerels (rastrelliger brachysoma) obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of v. parahaemolyticus. out of 130 short mackerel samples, 116 (89.2%) were detected with the pres ...201728659901
involvement of a tachylectin-like gene and its protein in pathogenesis of acute hepatopancreatic necrosis disease (ahpnd) in the shrimp, penaeus monodon.a shrimp disease, the so-called acute hepatopancreatic necrosis disease (ahpnd) is caused by a specific strain of vibrio parahaemolyticus (vp) and it has resulted in significant losses to the global shrimp farming industry. in our previous study, three of tachylectin-like genes were cloned and characterized from the intestine of penaeus monodon, designated as penlectin5-1 (pl5-1), penlectin5-2 (pl5-2) and penlectin5-3 (pl5-3). these three genes all contain fibrinogen-related domain (fred). the e ...201728655576
transcriptome analysis of the hepatopancreas in exopalaemon carinicauda infected with an ahpnd-causing strain of vibrio parahaemolyticus.acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in multiple shrimp species throughout asia. in order to understand the immunological response of shrimp to infection by ahpnd-causing strain of v. parahaemolyticus (vpahpnd), the transcriptomic profiles of the hepatopancreas from severe ahpnd-infected (bs_g), ahpnd-survived (kb_g) and non-infected (pbs_g) exopalaemon carinicauda groups were obtained us ...201728648882
a comprehensive epidemiological research for clinical vibrio parahaemolyticus in shanghai.vibrio parahaemolyticus is one of the most important pathogen for seafood-borne gastroenteritis in shanghai and the rest of the world. a total of 42 v. parahaemolyticus strains were isolated from 1900 fecal specimens collected from patients in shanghai hospital presenting from january 2014 to december 2015. all isolates were evaluated for potential virulence factors [tdh, trh, and type three secretion system (t3ss) genes], typed using multilocus sequence typing (mlst) and screened for antimicrob ...201728642752
t3ss effector vopl inhibits the host ros response, promoting the intracellular survival of vibrio parahaemolyticus.the production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (nadph) oxidase complex is an important mechanism for control of invading pathogens. herein, we show that the gastrointestinal pathogen vibrio parahaemolyticus counteracts reactive oxygen species (ros) production using the type iii secretion system 2 (t3ss2) effector vopl. in the absence of vopl, intracellular v. parahaemolyticus undergoes ros-dependent filamentation, with concurrent limited growth ...201728640881
comparative transcriptome analysis of sinonovacula constricta in gills and hepatopancreas in response to vibrio parahaemolyticus infection.the razor clam sinonovacula constricta is an important economic species in china. however, bacterial pathogenic diseases limits s. constricta farming industry for large-scale production. in this study, de novo transcriptome sequencing was performed on s. constricta gills and hepatopancreas under vibrio parahaemolyticus challenge for 12 h and 48 h, respectively. transcripts assembly constructed 18,330 sequences, each of which was 500 bp long and functionally annotated, and 1781 and 490 transcript ...201728630016
survival of litopenaeus vannamei shrimp fed on diets supplemented with dunaliella sp. is improved after challenges by vibrio parahaemolyticus.survival of litopenaeus vannamei was evaluated during a vibrio parahaemolyticus infection. this bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (ems), also known as acute hepatopancreatic necrosis disease (ahpnd). control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. new methods have been proposed to control and prevent the impact of bacterial ...201728625840
pathogenicity profile of vibrio parahaemolyticus in farmed pacific white shrimp, penaeus vannamei.a pathobiological study was conducted using vibrio parahaemolyticus (vp) strain isolated from vibriosis affected shrimp (penaeus vannamei) farms in kancheepuram and thiruvallur districts of tamil nadu during august 2014 to february 2015. the isolate was identified based on the morphological, physiological, biochemical and molecular characters. ld50 value with intramuscular injection was determined as 2.6 × 10(4) cfu/shrimp and sequential pathology was studied giving 6.1 × 10(3) cfu/shrimp (ld25) ...201728606862
genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (vpacspa and vpacspd) in vibrio parahaemolyticus chn25 during low-temperature survival.vibrio parahaemolyticus causes serious seafood-borne gastroenteritis and death in humans. raw seafood is often subjected to post-harvest processing and low-temperature storage. to date, very little information is available regarding the biological functions of cold shock proteins (csps) in the low-temperature survival of the bacterium. in this study, we determined the complete genome sequence of v. parahaemolyticus chn25 (serotype: o5:kut). the two main csp-encoding genes (vpacspa and vpacspd) w ...201728583064
a natural vibrio parahaemolyticus pir(vp)a(-)b(+) mutant kills shrimp but produces no pir(vp) toxins or ahpnd lesions.acute hepatopancreatic necrosis disease (ahpnd) of shrimp is caused by vibrio parahaemolyticus (vp) isolates that harbor a pva plasmid encoding toxins pir(vp)a and pir(vp)b (vpahpnd). these are released from vpahpnd that colonize the shrimp stomach and produce pathognomonic ahpnd lesions (massive sloughing of hepatopancreatic tubule epithelial cells). pcr results indicated that vp isolate xn87 lacked pir(vp)a but carried pir(vp)b unexpectedly, western blot analysis of proteins from culture broth ...201728576761
spreading of pandemic vibrio parahaemolyticus o3:k6 and its serovariants: a re-analysis of strains isolated from multiple studies.in china, v. parahaemolyticus has been a leading cause of foodborne outbreaks and bacterial infectious diarrhea since the 1990s, and most infections have been associated with the pandemic v. parahaemolyticus o3:k6 and its serovariants. however, a comprehensive overview of the sero-prevalence and genetic diversity of the pandemic v. parahaemolyticus clone in china is lacking. to compensate for this deficiency, pandemic isolates in both clinical and environmental chinese samples collected from mul ...201728573108
induction of cellular differentiation and single cell imaging of vibrio parahaemolyticus swimmer and swarmer cells.the ability to study the intracellular localization of proteins is essential for the understanding of many cellular processes. in turn, this requires the ability to obtain single cells for fluorescence microscopy, which can be particularly challenging when imaging cells that exist within bacterial communities. for example, the human pathogen vibrio parahaemolyticus exists as short rod-shaped swimmer cells in liquid conditions that upon surface contact differentiate into a subpopulation of highly ...201728570527
different abundance and correlational patterns exist between total and presumed pathogenic vibrio vulnificus and v. parahaemolyticus in shellfish and waters along the north carolina coast.monitoring of vibrio vulnificus and v. parahaemolyticus abundance is pertinent due to the ability of these species to cause disease in humans through aquatic vectors. previously, we performed a multiyear investigation tracking vibrio spp. levels in five sites along the southeastern north carolina coast. from february 2013 to october 2015, total v. vulnificus and v. parahaemolyticus abundance was measured in water, oysters and clams. in the current study, pathogenic subpopulations were identified ...201728531284
gene expression of vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment.vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °c, a condition seldom found in the ocean. however, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. in this context, revealing how gene expression enables v. parahaemolyticus to adapt to this particular condition -common to almost all v. parahaemolyticus isolates- will improve our understan ...201728525966
molecular typing of vibrio parahaemolyticus strains isolated from mollusks in the north adriatic sea.vibrio parahaemolyticus is an emerging foodborne pathogen in the mediterranean, usually associated with shellfish consumption. the increase in the number of outbreaks in europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. the primary role of italy in europe's mollusk production, together with the fact that cases of infections with v. parahaemolyticus are not always notified to the european commun ...201728514180
science signaling podcast for 16 may 2017: vibrio rewires host cells.this podcast features a conversation with kim orth and nicole de nisco, authors of a research resource that appears in the 16 may 2017 issue of science signaling, about how the marine bacterium vibrio parahaemolyticus rewires host cell signaling networks. v. parahaemolyticus thrives in warm brackish waters and infects both shellfish and finfish. this bacterium causes gastroenteritis when humans consume contaminated seafood that is raw or undercooked. v. parahaemolyticus delivers virulence factor ...201728512146
the cytotoxic type 3 secretion system 1 of vibrio rewires host gene expression to subvert cell death and activate cell survival pathways.bacterial effectors potently manipulate host signaling pathways. the marine bacterium vibrio parahaemolyticus (v. para) delivers effectors into host cells through two type 3 secretion systems (t3sss). t3ss1 is vital for v. para survival in the environment, whereas t3ss2 causes acute gastroenteritis in human hosts. although the natural host is undefined, t3ss1 effectors attack highly conserved cellular processes and pathways to orchestrate nonapoptotic cell death. to understand how the concerted ...201728512145
new sequence types of vibrio parahaemolyticus isolated from a malaysian aquaculture pond, as revealed by whole-genome sequencing.the acquisition of photorhabdus insect-related (pir) toxin-like genes in vibrio parahaemolyticus has been linked to hepatopancreatic necrosis disease in shrimp. we report the whole-genome sequences of genetically virulent and avirulent v. parahaemolyticus isolated from a malaysian aquaculture pond and show that they represent previously unreported sequence types of v. parahaemolyticus.201728495773
isolation of bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by vibrio parahaemolyticus.acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by vibrio parahaemolyticus (ahpnd vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to photorhabdus insect-related toxins. we obtained 9 isolates of bdellovibrio and like organisms (balos) from water and sediment samples in thailand. using 16s rrna sequencing, all of the organisms were identified as bacteriovorax spp. and were able to attack all tested ahpnd vp isolates. in addition, ...201728492178
pathogenic vibrio species isolated from estuarine environments (ceará, brazil) - antimicrobial resistance and virulence potential profiles.detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. thus, vibrio diversity was investigated in four estuaries from state of ceará (pacoti, choró, pirangi and jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. isolation and identification were performed on tcbs agar (selective medium) and dichotomous ...201728489191
bile sensing: the activation of vibrio parahaemolyticus virulence.bacteria must develop resistance to various inhospitable conditions in order to survive in the human gastrointestinal tract. bile, which is secreted by the liver, and plays an important role in food digestion also has antimicrobial properties and is able to disrupt cellular homeostasis. paradoxically, although bile is one of the guts defenses, many studies have reported that bacteria such as vibrio parahaemolyticus can sense bile and use its presence as an environmental cue to upregulate virulen ...201728484445
effects of ambient exposure, refrigeration, and icing on vibrio vulnificus and vibrio parahaemolyticus abundances in oysters.vibrio vulnificus (vv) and v. parahaemolyticus (vp) illnesses are typically acquired through the consumption of raw molluscan shellfish, particularly oysters. as vibrio spp. are naturally-occurring bacteria, one means of mitigation of illness is achieved by limiting post-harvest growth. in this study, effects of ambient air storage, refrigeration, and icing of oysters on vibrio spp. abundances were examined at two sites in alabama (al) [dog river (dr) and cedar point (cp)] and one site in delawa ...201728482282
a sulfated galactans supplemented diet enhances the expression of immune genes and protects against vibrio parahaemolyticus infection in shrimp.a sulfated galactans (sg) supplemented diet was evaluated for the potential to stimulate immune activity in shrimp penaeus vannamei (p. vannamei). shrimp given the sg supplemented diet (0.5, 1 and 2% w/w) for 7 days showed enhanced expression of the downstream signaling mediator of lipopolysaccharide and β-1,3-glucan binding protein (lgbp) and immune related genes including p-nf-κb, imd, ikkβ and ikkε, antimicrobial peptide pen-4, propo-i and ii. following immersion with vibrio parahaemolyticus ...201728442419
three new piscidins from orange-spotted grouper (epinephelus coioides): phylogeny, expression and functional characterization.the present study reports the identification, and characterization of three new putative piscidin paralogues, ecpis-2, ecpis-3 and ecpis-4, from orange-spotted grouper (epinephelus coioides). the cdna of the three piscidins with the 207, 216, and 231 nt open reading frame encoded respectively a 68-, 71-, and 76-amino acid preprotein consisting of the predicted signal peptide, and putative mature peptide and prodomain. the phylogenetic analysis indicated that multiple piscidin paralogues in one f ...201728433718
acute hepatopancreatic necrosis disease-causing vibrio parahaemolyticus strains maintain an antibacterial type vi secretion system with versatile effector repertoires.acute hepatopancreatic necrosis disease (ahpnd) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. ahpnd is caused by toxic strains of vibrio parahaemolyticus that have acquired a "selfish plasmid" encoding the deadly binary toxins pira(vp)/pirb(vp) to better understand the repertoire of virulence factors in ahpnd-causing v. parahaemolyticus, we conducted a comparative analysis using the genome sequences of the clinical strain rimd2210633 and of environmenta ...201728432099
recombinant pira-like toxin protects shrimp against challenge with vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease. 201728422302
plasmid dynamics in vibrio parahaemolyticus strains related to shrimp acute hepatopancreatic necrosis syndrome (ahpns).vibrio parahaemolyticus is a causative agent of acute hapatopancreatic necrosis syndrome (ahpns) which causes early mortality in white shrimp. emergence of ahpns has caused tremendous economic loss for aquaculture industry particularly in asia since 2010. previous studies reported that strains causing ahpns harbor a 69-kb plasmid with possession of virulence genes, pira and pirb. however, genetic variation of the 69-kb plasmid among ahpns related strains has not been investigated. this study aim ...201728404482
antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium bacillus subtilis mtcc 10403.brown seaweed anthophycus longifolius (turner) kützing (family sargassaceae) associated heterotrophic bacterium bacillus subtilis mtcc 10403 was found to be a potent isolate with broad range of antibacterial activity against important perceptive food pathogens vibrio parahaemolyticus, v. vulnificus, and aeromonas hydrophila. this bacterium was positive for polyketide synthetase gene (kc589397), and therefore, was selected to bioprospect specialized metabolites bearing polyketide backbone. bioact ...201728704687
antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves.fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ...201728698913
identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds.control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ...201728653930
antibacterial and antifungal activities of spices.infectious diseases caused by pathogens and food poisoning caused by spoilage microorganisms are threatening human health all over the world. the efficacies of some antimicrobial agents, which are currently used to extend shelf-life and increase the safety of food products in food industry and to inhibit disease-causing microorganisms in medicine, have been weakened by microbial resistance. therefore, new antimicrobial agents that could overcome this resistance need to be discovered. many spices ...201728621716
development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens.bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ...201728505584
seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium.seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ...201728733150
[growth and survival of total and pathogenic vibrio parahaemolyticus in american oyster (crassostrea virginica) under cold storage].to quantify vibrio parahaemolyticus densities in american oyster (crassostrea virginica) under cold storage.201726302123
prevalence of vibrio parahaemolyticus in seafood products from hypermarkets in shanghai.vibrio parahaemolyticus is an important gastroenteritis pathogen contaminating seafood in china. in this study a total of 992 seafood samples from major hypermarkets in shanghai were monitored for prevalence and burden of v. parahaemolyticus from january 2011 to december 2012. additionally, appropriate probability distributions for describing v. parahaemolyticus concentrations were assessed based on these surveillance data.201726991741
shrimp ahpnd-causing plasmids encoding the pirab toxins as mediated by pirab-tn903 are prevalent in various vibrio species.acute hepatopancreatic necrosis disease (ahpnd) is a newly emerging shrimp disease caused by pirab toxins encoded by a plasmid found in vibrio parahaemolyticus. the pirab toxins are the homologs of the photorhabdus insect-related (pir) toxins. here, we report the complete sequences of the ahpnd-causing plasmid isolated from v. owensii, as well as those of its 11 siblings (pvh family). in addition, we also included 13 related plasmids (pvh-r family) without the pirab genes isolated from a variety ...201728169338
calr is required for the expression of t6ss2 and the adhesion of vibrio parahaemolyticus to hela cells.vibrio parahaemolyticus expresses one major virulence determinant t6ss2, which is constituted into three putative operons, i.e., vpa1027-1024, vpa1043-1028, and vpa1044-1046. calr, a lysr-type transcriptional regulator, was originally identified as a repressor of the swarming motility and t3ss1 gene expression. as shown in this study, calr binds to the promoter-proximal region of each of the three operons to activate their transcription, and moreover, calr activates the adhesion of v. parahaemol ...201728378143
kinetics of the inactivation of vibrio parahaemolyticus in weakly acidic sodium chlorite solution.the kinetics of the inactivation of vibrio parahaemolyticus in sodium chlorite (naclo2) solution was studied in the weakly acidic ph range of 4.0 to 6.5 and at various temperatures. the logarithmic reduction of the survival ratio depended on the concentration-time product, and all the inactivation curves showed a linear reduction phase. the first-order inactivation rate constant (k) increased by approximately twice for every 0.44 unit fall in ph. during the inactivation experiments, no formation ...201728367867
development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of salmonella spp. and vibrio parahaemolyticus.rapid detection of food-borne pathogens is important in the food industry, to monitor and prevent the spread of these pathogens through contaminated food products. we therefore established a multiplex real-time loop-mediated isothermal amplification (lamp) assay to simultaneously detect and distinguish salmonella spp. and vibrio parahaemolyticus dna in a single reaction. two target sequences, one specific for salmonella and the other specific for vibrio parahaemolyticus, were amplified by specif ...201728349967
defining a core genome multilocus sequence typing scheme for the global epidemiology of vibrio parahaemolyticus.vibrio parahaemolyticus is an important human foodborne pathogen whose transmission is associated with the consumption of contaminated seafood with a growing number of infections reported over recent years worldwide. a multilocus sequence typing (mlst) database for v. parahaemolyticus was created in 2008 and a large number of clones have been identified causing severe outbreaks worldwide (st3), recurrent outbreaks in certain regions (e.g., st36) or spreading to other regions where they are non-e ...201728330888
transcriptional regulation of cpsq-mfpabc and mfpabc by calr in vibrio parahaemolyticus.the cpsq-mfpabc locus is transcribed as two operons, i.e., cpsq-mfpabc and mfpabc, in vibrio parahaemolyticus, and both of them are all required for biofilm formation. calr belongs to the lysr-type transcriptional regulator family, and was originally identified as a repressor of the swarming motility and t3ss1 genes expression in v. parahaemolyticus. in the present work, a combination of qrt-pcr, primer extension, lacz fusion expression, electrophoretic mobility shift assay, and dnase i footprin ...201728318117
characterization of vibrio parahaemolyticus isolated from oysters in korea: resistance to various antibiotics and prevalence of virulence genes.vibrio parahaemolyticus, found frequently in oysters, is the most prevalent gastroenteritis-causing pathogen in korea and in several other asian countries. this study monitored changes in the environmental parameters and occurrence of v. parahaemolyticus in oyster aquaculture sites. of the 44 presumed v. parahaemolyticus isolates obtained, when tested against 16 antibiotics, 90.9, 86.4, and 75.0% of the 44 isolates exhibited resistance to vancomycin, ampicillin, and streptomycin, respectively. p ...201728279505
vibrio parahaemolyticus calr down regulates the thermostable direct hemolysin (tdh) gene transcription and thereby inhibits hemolytic activity.tdh, encoded by tdh gene, is a major virulent determinant of v. parahaemolyticus that controls various biological activities, such as hemolytic activity, cytotoxicity, and enterotoxicity. the hemolytic activity on wagatsuma agar ascribed to tdh is called kanagawa phenomenon (kp). all kp positive strains contain tdh1 and tdh2 genes, but tdh2 is predominantly responsible for kp. calr is a regulatory protein that was originally identified as a repressor of swarming motility and t3ss1 gene expressio ...201728268179
an invertebrate sting from shrimp activates an innate immune defense against bacterial infection.it has been proposed that invertebrate stimulators of interferon genes (stings) do not take part in the innate immune response to infection. herein, we identified a new sting homolog from pacific white shrimp litopenaeus vannamei (lvsting). some amino acids crucial for recognizing cyclic dinucleotides in mammals are highly conserved in lvsting. moreover, lvsting expression can be robustly induced by challenge with the gram-negative bacteria vibrio parahaemolyticus. silencing of lvsting contribut ...201728236646
a simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus.an earlier electrochemical mechanism of dna detection was adapted and specified for the detection of vibrio parahaemolyticus in real samples. the reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. detection was assessed using a microprocessor to measure current response under controlled potential. the fabricated sensor was able to specifically distinguish complementary, non-comple ...201728205102
ecological fitness and virulence features of vibrio parahaemolyticus in estuarine environments.vibrio parahaemolyticus is a commonly encountered and highly successful organism in marine ecosystems. it is a fast-growing, extremely versatile copiotroph that is active over a very broad range of conditions. it frequently occurs suspended in the water column (often attached to particles or zooplankton), and is a proficient colonist of submerged surfaces. this organism is an important pathogen of animals ranging from microcrustaceans to humans and is a causative agent of seafood-associated food ...201728144705
genetic and serological identification of three vibrio parahaemolyticus strains as candidates for novel provisional o serotypes.vibrio parahaemolyticus is a gram-negative, halophilic vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. to date, 13 o serotypes and 71 k serotypes of v. parahaemolyticus have been identified. however, untypeable v. parahaemolyticus strains are frequently found during routine detection, indicating that other forms of serotypes exist and suggesting the necessity for extension of the antigen ...201728129570
detection, enumeration, and isolation of vibrio parahaemolyticus and v. vulnificus from seafood: development of a multidisciplinary protocol.vibrio parahaemolyticus and v. vulnificus are bacterial foodborne pathogens that can cause illnesses in humans after ingestion or exposure to contaminated seafood or coastal waters. a procedure that combines microbiological, biochemical, and molecular methods was designed and optimized for the detection, enumeration, isolation, and characterization of these clinically significant vibrio spp. initially, microbiological culturing is used to resuscitate and isolate presumptive vibrio spp. from chil ...201728118133
transcriptome profiles of the clam meretrix petechialis hepatopancreas in response to vibrio infection.microbial diseases have received much attention due to their enormous destruction of aquaculture, and vibrio parahaemolyticus is one of the main pathogens that cause bacterial disease in the clam meretrix petechialis. to better understand the molecular mechanisms of the immune response to vibrio in m. petechialis, rna-seq was applied to explore global expression changes of hepatopancreas from this clam after vibrio challenge. there were 199,318,966 clean reads obtained by illumina sequencing, wh ...201728110034
high salinity relaying to reduce vibrio parahaemolyticus and vibrio vulnificus in chesapeake bay oysters (crassostrea virginica).cases of vibrio infections in the united states have tripled from 1996 to 2009 and these infections are most often associated with the consumption of seafood, particularly oysters (crassostrea virginica). information is needed on how to reduce numbers of vibrio parahaemolyticus and vibrio vulnificus in bi-valve molluscan shellfish (for example, oysters). the purpose of this study was to evaluate the effectiveness of high salinity relaying or treatment in recirculating aquaculture systems (rass) ...201728099766
genetic analysis of vibrio parahaemolyticus o3:k6 strains that have been isolated in mexico since 1998.vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. environmental and clinical toxigenic strains of v. parahaemolyticus that were isolated in mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the o3:k6 pandemic clone, and their genetic relationship to strains that are r ...201728099500
establishment and validation of rna-based predictive models for understanding survival of vibrio parahaemolyticus in oysters stored at low temperatures.this study developed rna-based predictive models describing the survival of vibrio parahaemolyticus in eastern oysters (crassostrea virginica) during storage at 0, 4, and 10°c. postharvested oysters were inoculated with a cocktail of five v. parahaemolyticus strains and were then stored at 0, 4, and 10°c for 21 or 11 days. a real-time reverse transcription-pcr (rt-pcr) assay targeting expression of the tlh gene was used to evaluate the number of surviving v. parahaemolyticus cells, which was the ...201728087532
vibrio parahaemolyticus o4:k8 forms a potential predominant clone in southern china as detected by whole-genome sequence analysis.vibrio parahaemolyticus has been the most common food-borne pathogen in southern china, especially the o3:k6 pandemic clone and its serovariants. recently, the serotype o4:k8 became more and more prevalent in southern china, which was different from the o3:k6 pandemic clone. thus, the aim of the present work was to elucidate the molecular characteristics of the o4:k8. some o3:k6 pandemic clone and its serovariants isolated in the same period were selected for comparative analysis, which were sti ...201728073082
quorum sensing regulators are required for metabolic fitness in vibrio parahaemolyticus.quorum sensing (qs) is a process by which bacteria alter gene expression in response to cell density changes. in vibrio species, at low cell density, the sigma 54-dependent response regulator luxo is active and regulates the two qs master regulators apha, which is induced, and opar, which is repressed. at high cell density the opposite occurs: luxo is inactive, and therefore opar is induced while apha is repressed. in vibrio parahaemolyticus, a significant enteric pathogen of humans, the roles o ...201728069817
impact of farm management on expression of early mortality syndrome/acute hepatopancreatic necrosis disease (ems/ahpnd) on penaeid shrimp farms in thailand.asian shrimp farming industry has experienced massive production losses due to a disease caused by toxins of vibrio bacteria, known as early mortality syndrome/acute hepatopancreatic necrosis disease (ems/ahpnd) for the last 5 years. the disease can cause up to 100% cumulative pond mortality within a week. the objective of this study was to identify factors associated with ahpnd occurrence on shrimp farms. a case-control study was carried out on shrimp farms in four provinces of thailand. factor ...201727594170
differences in abundances of total vibrio spp., v. vulnificus, and v. parahaemolyticus in clams and oysters in north carolina.filter feeding shellfish can concentrate pathogenic bacteria, including vibrio vulnificus and vibrio parahaemolyticus, as much as 100-fold from the overlying water. these shellfish, especially clams and oysters, are often consumed raw, providing a route of entry for concentrated doses of pathogenic bacteria into the human body. the numbers of foodborne infections with these microbes are increasing, and a better understanding of the conditions that might trigger elevated concentrations of these b ...201727793822
metabolic responses of haliotis diversicolor to vibrio parahaemolyticus infection.vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone haliotis diversicolor. elucidation of metabolic mechanisms of abalones in responding to v. parahemolyticus infection is essential for controlling the epidemic. in this work, (1)h nmr-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of h. diver ...201727890800
enhancing gilthead seabream immune status and protection against bacterial challenge by means of antigens derived from vibrio parahaemolyticus.in an attempt to control the proliferation of the pathogenic bacterium vibrio parahaemolyticus in gilthead seabream (sparus aurata), the immunostimulant effect of lysate and toxa from this bacterium was evaluated. fish were intraperitoneally injected twice (first injection, day 1 of the experiment; second injection, day 7) and sampled after one week (on days 8 and 15). afterwards, all fish specimens were experimentally infected with v. parahaemolyticus and mortality was recovered for 1 week. fis ...201727890799
the role of ficolin-like protein (pcflp1) in the antibacterial immunity of red swamp crayfish (procambarus clarkii).in invertebrates, ficolin-like proteins (flps) play important roles in innate immunity against pathogens. previous studies primarily investigated the functions of flps in immune recognition, activation, and regulation. however, limited research has examined the functions of flps as immune effectors. in this work, a ficolin-like protein was identified in red swam crayfish (procambarus clarkii) and designated as pcflp1. quantitative rt-pcr and western blot were employed to analyze the distribution ...201727888717
hsp70 and hsp90 are involved in shrimp penaeus vannamei tolerance to ahpnd-causing strain of vibrio parahaemolyticus after non-lethal heat shock.acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in farmed penaeid shrimp throughout asia. previous studies reported that a non-lethal heat shock (nlhs) could enhance disease tolerance in aquatic animals. here, we investigate whether the nlhs could enhance the survival of shrimp penaeusvannamei upon challenge with an ahpnd-causing strain of v. parahaemolyticus (vpahpnd). two nlhs conditions, acute an ...201727888131
fatty acid synthase plays a positive role in shrimp immune responses against vibrio parahaemolyticus infection.fatty acid synthase (fas) is an important enzyme that catalyzes the synthesis of fatty acids. in this study, the role of the fas gene from pacific white shrimp litopenaeus vannamei (lvfas) in immune responses against vibrio parahaemolyticus infection was studied. the expression of lvfas could be up-regulated upon infection of v. parahaemolyticus and stimulation of lipopolysaccharide and poly (i:c). the promoter of lvfas was predicted to harbor a nf-κb binding site and dual-luciferase reporter as ...201727903451
an integrated rotary microfluidic system with dna extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics.point-of-care (poc) molecular diagnostics plays a pivotal role for the prevention and treatment of infectious diseases. in spite of recent advancement in microfluidic based poc devices, there are still rooms for development to realize rapid, automatic and cost-effective sample-to-result genetic analysis. in this study, we propose an integrated rotary microfluidic system that is capable of performing glass microbead based dna extraction, loop mediated isothermal amplification (lamp), and colorime ...201728043075
alpha-2-macroglobulin is a modulator of prophenoloxidase system in pacific white shrimp litopenaeus vannamai.the shrimp multifunctional protein alpha-2-macroglobulin (a2m) is abundantly expressed in plasma, highly up-regulated upon microbial infection and involved in several immune pathways such as blood clotting system, phagocytosis and melanization. herein, the function of lva2m from litopenaeus vannamei on the prophenoloxidase (propo) system is reported. the recombinant (r)lva2m produced strongly and specifically inhibited trypsin and the po activity in shrimp plasma in a dose-dependent manner. sile ...201728027986
sequence type 631 vibrio parahaemolyticus, an emerging foodborne pathogen in north america. 201727974540
molecular cloning and expression analysis of a fish specific interferon regulatory factor, irf11, in orange spotted grouper, epinephelus coioides.interferon regulatory factors (irfs) are transcription mediators which play vital roles in multiple biological processes, such as antiviral defense, immune response, cell growth regulation and apoptosis. a fish specific irf, termed irf11, has been identified in previous study through searching fish genome databases. herein, a transcript of irf11, ecirf11 was cloned from orange-spotted grouper, epinephelus coioides. the ecirf11 cdna sequence has 1573 bp in length, encoding a putative protein of 2 ...201727932023
sensitive immunoassay-based detection of vibrio parahaemolyticus using capture and labeling particles in a stationary liquid phase lab-on-a-chip.in the present study, a method was developed for detection of vibrio parahaemolyticus based on a stationary liquid phase lab-on-a-chip (slp loc). the present slp loc comprises a sample chamber, washing chamber, and detection chamber connected by two channels. the method utilizes two types of particles: capture particles (cps), which are magnetic nanoparticles functionalized with antibody; and labeling particles (lps), which are silica nanoparticles functionalized with horseradish peroxidase and ...201727923189
disulphide bond restrains the c-terminal region of thermostable direct hemolysin during folding to promote oligomerization.pore-forming toxins (pfts) are typically produced as water-soluble monomers, which upon interacting with target cells assemble into transmembrane oligomeric pores. vibrio parahaemolyticus thermostable direct hemolysin (tdh) is an atypical pft that exists as a tetramer in solution, prior to membrane binding. the tdh structure highlights a core β-sandwich domain similar to those found in the eukaryotic actinoporin family of pfts. however, the tdh structure harbors an extended c-terminal region (ct ...201727784764
the hot oyster: levels of virulent vibrio parahaemolyticus strains in individual oysters.vibrio parahaemolyticus is the leading cause of seafood-associated gastroenteritis and is most commonly transmitted by raw oysters. consequently, detection of virulent strains of this organism in oysters is a primary concern for seafood safety. vibrio parahaemolyticus levels were determined in 110 individual oysters harvested from two sampling sites in sc, usa. the majority of oysters (98%) contained low levels of presumptive v. parahaemolyticus however, two healthy oysters contained presumptive ...201727827805
debaryomyces hansenii up regulates superoxide dismutase gene expression and enhances the immune response and survival in pacific red snapper (lutjanus peru) leukocytes after vibrio parahaemolyticus infection.application of yeast is increasing to improve welfare and promotes growth in aquaculture. the halotolerant yeast debaryomyces hansenii is normally a non-pathogenic yeast with probiotic properties and potential source of antioxidant enzymes as superoxide dismutase. here, first, we characterized the sequence features of mnsod and iccu/znsod from pacific red snapper, and second, we evaluated the potential antioxidant immune responses of the marine yeast debaryomyces hansenii strain cbs004 in leukoc ...201728126556
control of biofilm forming clinically important bacteria by green synthesized zno nanoparticles and its ecotoxicity on ceriodaphnia cornuta.zinc oxide nanoparticles were synthesized using the aqueous leaf extracts of plectranthus barbatus (plb-zno nps) and characterized by uv-visible spectroscopy, xrd, ftir, sem and eds. uv-visible spectra recorded the absorbance peak of plb-zno nps at 343 nm. sem analyses showed the spherical shape of plb-zno nps with the particle size between 30 and 60 nm. plb-zno nps exhibited antibacterial and antibiofilm activity against gram positive bacillus subtilis at all tested concentrations. in contrast, ...201728330748
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters.periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ...201728235124
microbiological quality and prevalence of β-lactam antibiotic resistance genes in oysters ( crassostrea rhizophorae ).the microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. the objective of this study was to assess the bacterial composition of the edible oyster crassostrea rhizophorae in urban and preserved estuaries. particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blatem, blashv, and blakpc) via quantitative pcr ...201728207310
vibrio phage kvp40 encodes a functional nad+ salvage pathway.the genome of t4-type vibrio bacteriophage kvp40 has five genes predicted to encode proteins of pyridine nucleotide metabolism, of which two, nadv and natv, would suffice for an nad(+) salvage pathway. nadv is an apparent nicotinamide phosphoribosyltransferase (namprtase) and natv is an apparent bifunctional nicotinamide mononucleotide adenylyltransferase (nmnatase) and nicotinamide-adenine dinucleotide pyrophosphatase (nudix hydrolase). genes encoding the predicted salvage pathway were cloned, ...201728167526
kinneretia thg-sqi4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy.simple, facile, effective approach for the biosynthesis of silver nanoparticles using kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. kinneretia thg-sqi4 has been isolated from soil sample collected from shangqui, china. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (uv-vis), field emission transmission electron microscopy (fe-tem), energy dispersive x-ray spectroscopy (edx), elemental mapp ...201728211298
raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets.microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ...201727747902
biosynthesis of silver nanoparticles by novosphingobium sp. thg-c3 and their antimicrobial potential.the present study described biosynthesis of silver nanoparticles (agnps) using a bacterial strain novosphingobium sp. thg-c3, isolated from soil, and their application in antibacterial activity. the maximum absorbance values of the synthesized agnps was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. x-ray diffraction pattern corresponding to planes ( ...201727145847
biological therapeutics of pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and mcf-7 breast cancer cells.the overuse of antimicrobics and drugs has led to the development of resistance in a number of pathogens and parasites, which leads to great concerns for human health and the environment. furthermore, breast cancer is the second most common cause of cancer death in women. mcf-7 is a widely used epithelial cancer cell line, derived from breast adenocarcinoma for in vitro breast cancer studies, since the cell line has retained several ideal characteristics particular to the mammary epithelium. in ...201728115262
o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum.the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ...201727624095
transcriptome analysis reveals comprehensive insights into the early immune response of large yellow croaker (larimichthys crocea) induced by trivalent bacterial vaccine.vaccination is an effective and safe strategy for combating bacterial diseases in fish, but the mechanisms underlying the early immune response after vaccination remain to be elucidated. in the present study, we used rna-seq technology to perform transcriptome analysis of spleens from large yellow croaker (larimichthys crocea) induced by inactivated trivalent bacterial vaccine (vibrio parahaemolyticus, vibrio alginolyticus and aeromonas hydrophila). a total of 2,789 or 1,511 differentially expre ...201728135311
induction of type i interferons in response to bacterial stimuli in large yellow croaker larimichthys crocea.in addition to the crucial roles in coordinating antiviral immune responses, type i interferons (ifns) also play a role in the host immunity against bacterial pathogens. our previous study identified two type i ifns from large yellow croaker larimichthys croaea(lc), lcifnd and lcifnh, and showed their strong induction by poly(i:c) and antiviral activities. in the present study, both lcifnd and lcifnh were found to be rapidly induced in head kidney and spleen by mixed bacteria of vibrio alginolyt ...201728119146
designation of pathogenic resistant bacteria in the sparusaurata sea collected in tunisia coastlines: correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics.vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including vibrio parahaemolyticus and vibrio alginolyticus. in this study, we reported the phenotypic and molecular characterization of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from wild and farm sea bream (sparus aurata l.) along the tunisian coast from december 2015 to april ...201728062288
season-specific occurrence of potentially pathogenic vibrio spp. on the southern coast of south korea.vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. in this study, we aimed to isolate and observe the distribution of enteropathogenic vibrio spp. from environments of the southern coast of south korea over a season cycle. a total of 10,983 isolates of vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southw ...201727836844
responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus.marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases.201728390964
immune response and antioxidant status of portunus trituberculatus inoculated with pathogens.the white spot syndrome virus (wssv), vibrio parahaemolyticus and v. alginolyticus are serious epidemic pathogen affecting the cultured portunus trituberculatus and resulted in severe economic losses to local farmers. the immune and antioxidant systems are believed to be closely involved in host responses to pathogens in aerobic animals, including crustaceans. in order to explore such host-pathogen interactions in the early stage of infection in p. trituberculatus, the mrna transcript levels of ...201728235638
a novel anti-lipopolysaccharide factor spalf6 in mud crab scylla paramamosain exhibiting different antimicrobial activity from its single amino acid mutant.in crustaceans, anti-lipopolysaccharide factors (alfs) are important immune effectors that have sequence diversity and exhibit broad antimicrobial activities. in this study, we characterized a novel alf homolog spalf6 from mud crab scylla paramamosain and its variant spalf6-v, which was generated by mutations of two amino acids (h46 to r and a110 to p) due to the presence of two single nucleotide polymorphisms (snps). spalf6 was an anionic peptide with isoelectric point (pi) 6.79, whereas spalf6 ...201728232132
hctoll3 was involved in anti-vibrio defense in freshwater pearl mussel, hyriopsis cumingii.toll-like receptors (tlrs) play an important role in the activation of innate immune response but their functions in bivalves remain largely unknown. in this study, we identified a tlr from the freshwater pearl mussel hyriopsis cumingii (hctoll3) and investigated its functions in immunity. the full-length cdna of hctoll3 is 3852 bp and includes an open reading frame (orf) of 3228 bp that encodes a polypeptide of 1075 amino acids. the predicted hctoll3 protein shares similar structural characteri ...201728214599
identification and functional analysis of a tep gene from a crustacean reveals its transcriptional regulation mediated by nf-κb and jnk pathways and its broad protective roles against multiple pathogens.thioester-containing proteins (teps) are present in a wide range of species from deuterostomes to protostomes and are thought to be involved in innate immunity. in the current study, a tep gene homologous to insect teps (itep) from the crustacean litopenaeus vannamei, named lvtep1, is cloned and functionally characterized. the open reading frame (orf) of lvtep1 is 4383 bp in length, encoding a polypeptide of 1460 amino acids with a calculated molecular weight of 161.1 kda lvtep1, which is most s ...201728069434
occurrence and virulence properties of vibrio and salinivibrio isolates from tropical lagoons of the southern caribbean sea.the vibrionaceae are gram-negative bacteria present in marine and estuarine environments worldwide, including several species known as important pathogens to humans and aquatic organisms. the aim of this research was to investigate the occurrence and virulence properties of vibrio and salinivibrio isolated from lagoons at cuare wildlife refuge and margarita island in the southern caribbean sea. water, plankton and oyster samples were collected during october 2011 and march 2012 and examined by s ...201728324231
biochemical basis for activation of virulence genes by bile salts in vibrio parahaemolyticus.bile salts act as a stressor to bacteria that transit the intestinal tract. enteric pathogens have hijacked bile as an intestinal signal to regulate virulence factors. we recently demonstrated that vibrio parahemolyticus senses bile salts via a heterodimeric receptor formed by the periplasmic domains of inner-membrane proteins vtra and vtrc. crystal structures of the periplasmic complex reveal that vtra and vtrc form a β-barrel that binds bile salts in its hydrophobic interior to activate the vt ...201728129014
biogeochemical and hydrological drivers of the dynamics of vibrio species in two patagonian estuaries.the ecology of the most relevant vibrio species for human health and their relation to water quality and biogeochemistry were studied in two estuaries in argentinian patagonia. vibrio cholerae and vibrio parahaemolyticus were reported in >29% of cases at the río colorado and río negro estuaries. neither the pandemic serogroups of vibrio cholerae o1, vibrio cholerae o139 nor the cholera toxin gene were detected in this study. however, several strains of v. cholerae (not o1 or o139) are able to ca ...201727871750
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