Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| experimental murine hyalohyphomycosis with soil-derived isolates of fusarium solani. | two strains of soil-borne fusarium solani, both characterized for their ability to produce cyclosporin a and c, were examined for their pathogenicity in severe combined immunodeficiency (scid) and balb/c male mice. intravenous (i.v.) infections with f. solani conidia were performed. no mortality was observed after infection with 0.3-1.6 x 10(7) cfu per mouse in scid and balb/c mice. when mice were infected with 0.8-1.5 x 10(6) cfu per mouse and 2 days later with 1.2-1.9 x 10(6) cfu per mouse, 28 ... | 2003 | 12964716 |
| monacrosporium janus sp. nov., a new nematode-trapping hyphomycete parasitizing sclerotia and hyphae of sclerotinia sclerotiorum. | during an investigation of mycoparasitic fungi on sclerotia of sclerotinia sclerotiorum in china, a new fungal species was consistently encountered and isolated from natural soils taken from soybean fields of shandong and jiangsu provinces. the fungus is featured by its sphaeroid conidia with 1-2 transverse septa, but mostly (>65%) with only one septum at the base. it resembles monacrosporium indicum, m. sphaeroides and m. sinense, but can be distinguished from the first two species by lack of b ... | 2003 | 12967217 |
| integration of production and aqueous two-phase systems extraction of extracellular fusarium solani pisi cutinase fusion proteins. | genetic engineering was integrated with the production and purification of fusarium solani pisi cutinases, in order to obtain the highest amount of enzyme activity units, after purification. an aqueous two-phase system (atps) of polyethylene glycol 3350, dipotassium phosphate and whole broth was used for the extraction of three extracellular cutinases expressed in saccharomyces cerevisiae. the production/extraction process was evaluated regarding cutinases secretion in the medium, partition beha ... | 2003 | 12413786 |
| direct quantification of fungal dna from soil substrate using real-time pcr. | detection and quantification of genomic dna from two ecologically different fungi, the plant pathogen fusarium solani f. sp. phaseoli and the arbuscular mycorrhizal fungus glomus intraradices, was achieved from soil substrate. specific primers targeting a 362-bp fragment from the ssu rrna gene region of g. intraradices and a 562-bp fragment from the f. solani f. sp. phaseoli translation elongation factor 1 alpha gene were used in real-time polymerase chain reaction (pcr) assays conjugated with t ... | 2003 | 12609725 |
| tryptophan-tagged cutinase studied by steady state fluorescence for understanding of tag interactions in aqueous two-phase systems. | genetic engineering has been used to construct fusion proteins of fusarium solani pisi cutinase and tryptophan-based tags, expressed in saccharomyces cerevisiae, to increase the partitioning in aqueous two-phase systems. the separation systems were composed of thermoseparating polymers (random copolymers of ethylene oxide and propylene oxide, eopo) and detergents (c(12)eo(n)). in this study, the fluorescence behaviour of the peptide-tagged protein, free peptide tag and tryptophan was investigate ... | 2003 | 12637012 |
| a diffusible factor from arbuscular mycorrhizal fungi induces symbiosis-specific mtenod11 expression in roots of medicago truncatula. | using dual cultures of arbuscular mycorrhizal (am) fungi and medicago truncatula separated by a physical barrier, we demonstrate that hyphae from germinating spores produce a diffusible factor that is perceived by roots in the absence of direct physical contact. this am factor elicits expression of the nod factor-inducible gene mtenod11, visualized using a pmtenod11-gusa reporter. transgene induction occurs primarily in the root cortex, with expression stretching from the zone of root hair emerg ... | 2003 | 12644648 |
| towards a cost effective strategy for cutinase production by a recombinant saccharomyces cerevisiae: strain physiological aspects. | although the physiology and metabolism of the growth of yeast strains has been extensively studied, many questions remain unanswered where the induced production of a recombinant protein is concerned. this work addresses the production of a fusarium solani pisi cutinase by a recombinant saccharomyces cerevisiae strain induced through the use of a galactose promoter. the strain is able to metabolise the inducer, galactose, which is a much more expensive carbon source than glucose. both the transp ... | 2003 | 12658517 |
| purification and characterization of a cross-reactive 45-kd major allergen of fusarium solani. | fusarium solani (fs) is an important source of fungal allergen. a 45-kd major allergen of fs showed reactivity with patients' sera sensitive to many fungi. | 2003 | 12660423 |
| mycetoma of the foot caused by fusarium solani: identification of the etiologic agent by dna sequencing. | we report a case of fusarium solani mycetoma of the foot that could not be diagnosed by culture, but was correctly identified after amplification and sequence analysis of fungal internal transcribed spacers 1 and 2 and 5.8s ribosomal dna regions. | 2003 | 12682194 |
| physicochemical characterization and antimicrobial properties of rhamnolipids produced by pseudomonas aeruginosa 47t2 ncbim 40044. | pseudomonas aeruginosa 47t2, grown in submerged culture with waste frying oil as a carbon source, produced a mixture of rhamnolipids with surface activity. up to 11 rhamnolipid homologs (rha-rha-c(8)-c(10); rha-c(10)-c(8)/rha-c(8)-c(10);rha-rha-c(8)-c(12:1); rha-rha-c(10)-c(10); rha-rha-c(10)-c(12:1); rha-c(10)-c(10); rha-rha-c(10)-c(12)/rha-rha-c(12)-c(10); rha-c(10)-c(12:1)/rha-c(12:1)-c(10); rha-rha-c(12:1)-c(12); rha-rha-c(10)-c(14:1); rha-c(10)-c(12)/rha-c(12)-c(10)) were isolated from cult ... | 2003 | 12474254 |
| effects of codon usage versus putative 5'-mrna structure on the expression of fusarium solani cutinase in the escherichia coli cytoplasm. | matching the codon usage of recombinant genes to that of the expression host is a common strategy for increasing the expression of heterologous proteins in bacteria. however, while developing a cytoplasmic expression system for fusarium solani cutinase in escherichia coli, we found that altering codons to those preferred by e. coli led to significantly lower expression compared to the wild-type fungal gene, despite the presence of several rare e. coli codons in the fungal sequence. on the other ... | 2003 | 12509995 |
| localized cutaneous hyalohyphomycosis caused by a fusarium species infection in a renal transplant patient. | fusariosis is a hyalohyphomycosis due to fusarium species that mainly occurs in immunocompromised hosts. the clinical spectrum of fusarium infection comprises localized and disseminated forms. a case of localized cutaneous fusariosis caused by fusarium solani in a renal transplant patient is described, and the skin manifestations of the disease are discussed. | 2003 | 12574313 |
| anatomy of a regimen: consideration of multipurpose solutions during non-compliant use. | consumers are often non-compliant with instructions for contact lens care products. this study explores the antimicrobial capacity of multipurpose solutions using variable use conditions. opti-free express (alcon) multi-purpose disinfecting solution with polyquad (alcon) and aldox (alcon) antimicrobial system and products containing phmb (renu multiplus, solo-care, and complete multipurpose solutions) were evaluated. products were challenged with fusarium solani, candida albicans, serratia marce ... | 2003 | 16303493 |
| contribution of regimen steps to disinfection of hydrophilic contact lenses. | the effect of regimen steps on overall performance of contact lens disinfection processes was evaluated. hydrophilic lenses were inoculated with staphylococcus aureus, pseudomonas aeruginosa, serratia marcescens, candida albicans, or fusarium solani. contributions of regimen steps and rinse volumes to disinfection abilities of opti-free express, solo-care plus (ciba vision), complete (allergan), and renu multiplus (bausch & lomb) multi-purpose solutions were examined. numbers of surviving microo ... | 2004 | 16303537 |
| purification and characterization of a major cross-reactive allergen from epicoccum purpurascens. | epicoccum purpurascens (formerly nigrum) (ep), is a ubiquitous saprophytic mould found both indoors and outdoors. several studies have reported sensitization to ep in 5-7% of different populations worldwide. the diagnosis of mould allergy requires a standardized fungal extract that contains all its important allergenic proteins. the crude allergen extract from ep was standardized earlier, however none of its allergens have been purified. | 2004 | 14976389 |
| recombinant saccharomyces cerevisiae strain triggers acetate production to fuel biosynthetic pathways. | although the metabolism and physiology of the growth of yeast strains has been extensively studied, many questions remain unanswered when the induced production of a recombinant protein is concerned. this work addresses the production of a fusarium solani pisi cutinase by a recombinant saccharomyces cerevisiae strain induced through the use of a galactose promoter. it was observed that whenever the strain needed to activate biosynthetic pathways, either for cutinase synthesis, or for the synthes ... | 2004 | 15063624 |
| site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex. | protein engineering strategies facilitating controlled and spontaneous assembly of macromolecular complexes are of great interest for the design of artificial multi-enzyme systems of pre-defined composition. here we have combined affinity proteins from different sources to achieve specific and reversible anchoring of affinity domain-tagged reporter proteins to a cellulose-anchored fusion protein. the design principle mimics the architecture of macromolecular cellulosome complexes produced by som ... | 2004 | 15066765 |
| mistletoe viscotoxins induce membrane permeabilization and spore death in phytopathogenic fungi. | viscotoxins (vts) are basic peptides expressed in mistletoe leaves, seeds and stems which have been shown to be cytotoxic to mammalian cells. the aim of this study was to analyse whether vts were able to control and/or inhibit the growth of phytopathogenic fungi to obtain a clue to their biological function. incubation of two vt isoforms, vta(3) and vtb, at a final concentration of 10 micro m resulted in a complete blockage of the germination of spores from three different pathogenic fungi. it w ... | 2004 | 15086811 |
| phenylpropanoids, phenylalanine ammonia lyase and peroxidases in elicitor-challenged cassava (manihot esculenta) suspension cells and leaves. | control of diseases in the key tropical staple, cassava, is dependent on resistant genotypes, but the innate mechanisms are unknown. the aim was to study phenylpropanoids and associated enzymes as possible defence components. | 2004 | 15145789 |
| potential ecotoxicological implication of methyl tert-butyl ether (mtbe) spills in the environment. | streptomyceticidal activity of methyl tert-butyl ether (mtbe) elucidated for the first time. adverse effect of mtbe, the gasoline additive, against 11 soil inhabitant streptomyces spp. isolates was investigated. mtbe, an octane enhancer is added to gasoline to reduce atmospheric concentrations of carbon monoxide and ozone. it contaminates soil and groundwater by fuel leaks and spills. streptomyces spp. are of the major contributors to the biological buffering of soils by exerting beneficial and ... | 2004 | 15673212 |
| fusarium solani endocarditis successfully treated with liposomal amphotericin b and voriconazole. | fungal infections caused by fusarium in the immunocompromised host are highly resistant to all antifungal agents. fusarium endocarditis is a rare and usually fatal disease. we report an immunocompromised child who survived fusarium solani endocarditis despite the in vitro resistance of the organism to all available antifungal agents. | 2004 | 15545866 |
| effect of voriconazole on a corneal abscess caused by fusarium. | to describe a case of corneal abscess caused by fusarium solani that did not respond to common antifungal agents. | 2004 | 15606478 |
| isatin-derived antibacterial and antifungal compounds and their transition metal complexes. | a series of isatins incorporating thiazole, thiadiazole, benzothiazole and p-toluene sulfonyl hydrazide moieties, along with their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by elemental analyses, molar conductances, magnetic moments, ir, nmr and electronic spectral data. these compounds have been screened for antibacterial activity against escherichia coli, bacillus subtillis, shigella flexneri, staphylococcus aureus, pseudomonas aeru ... | 2004 | 15648656 |
| pulmonary hyalohyphomycosis caused by fusarium spp in a kemp's ridley sea turtle (lepidochelys kempi): an immunohistochemical study. | to describe the microscopic and immunohistochemical findings in a case of pulmonary hyalohyphomycosis in a kemp's ridley sea turtle (lepidochelys kempi). | 2004 | 15768112 |
| antimicrobial and insecticidal protein isolated from seeds of clitoria ternatea, a tropical forage legume. | the tropical forage legume clitoria ternatea (l.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. it is resistant to a number of pathogens and pests. these important traits gave us reasons to look more closely at the plant. a highly basic small protein was purified from seeds of c. ternatea to homogeneity by using ultrafiltration with centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (ief). a single prot ... | 2004 | 15694280 |
| protein topology affects the appearance of intermediates during the folding of proteins with a flavodoxin-like fold. | the topology of a native protein influences the rate with which it is formed, but does topology affect the appearance of folding intermediates and their specific role in kinetic folding as well? this question is addressed by comparing the folding data recently obtained on apoflavodoxin from azotobacter vinelandii with those available on all three other alpha-beta parallel proteins the kinetic folding mechanism of which has been studied, i.e. anabaena apoflavodoxin, fusarium solani pisi cutinase ... | 2004 | 15829351 |
| vesicles as initial skin manifestation of disseminated fusariosis after non-myeloablative stem cell transplantation. | a 52-year-old man underwent non-myeloablative stem cell transplantation from his hla identical sister for the treatment of mantle cell lymphoma. on day 0, he developed a high-grade fever, watery diarrhea and vesicles scattered on the skin. well experienced dermatologists diagnosed these lesions as vzv reactivation. high dose antiviral agents were ineffective, and fusarium solani was cultured from his stool and sputum. systemic fusariosis progressed rapidly and he died of multiorgan failure on da ... | 2004 | 15160931 |
| onychomycosis caused by fusarium solani and fusarium oxysporum in são paulo, brazil. | fusarium species are common soil saprophytes and plant pathogens that have been frequently reported as etiologic agents of opportunistic infections in humans. we report eight cases of onychomycosis caused by fusarium solani (4) and fusarium oxysporum (4) in são paulo, brazil. these species were isolated from toenails in all cases. the infections were initially considered to be caused by dermatophytes. the clinical appearance of the affected toenails was leukonychia or distal subungual hyperkerat ... | 2004 | 15180157 |
| immunosuppression affects the severity of experimental fusarium solani keratitis. | we have established a mouse model of corneal fusariosis that permits the evaluation of fungal infection and pathogenesis. corneas of immunocompetent and cyclophosphamide-treated adult balb/c mice were topically inoculated with fusarium solani after corneal scarification. eyes were scored for corneal involvement daily for 8 days and at 2 weeks after infection. eyes were enucleated at various time points for quantitative fungal recovery and histopathological examination. an inoculum-dose response ... | 2004 | 15195260 |
| antifungal cobalt(ii), copper(ii), nickel(ii) and zinc(ii) complexes of furanyl-,thiophenyl-, pyrrolyl-, salicylyl- and pyridyl-derived cephalexins. | some novel cephalexin-derived furanyl, thiophenyl, pyrrolyl, salicylyl and pyridyl schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes have been synthesized and studied for their antifungal properties against trichophyton longifusus, candida albicans, aspergillus flavus, microsporum canis, fusarium solani and candida glaberata. the presence of metal ions in the investigated schiff's base complexes reported here lead to significant antifungal activity, whereas t ... | 2004 | 15202498 |
| [assessment of airborne mycoflora in critical areas of the principal hospital of cumaná, state of sucre, venezuela]. | the study of the nosocomial infections of fungic origin has attained importance in the last years, due to the rise in the number of patients that are inmunocompromised and susceptible to suffer this kind of infection. the objective of the present study was to evaluate the frequency of filamentous fungi and yeast, present in the environment of the intensive care unit, operating and newborn children rooms of the hospital universitario "antonio patricio de alcalá" (huapa) from the city of cumaná. s ... | 2004 | 15211981 |
| isoflavonoid accumulation in soybean hairy roots upon treatment with fusarium solani. | hairy roots were initiated from two soybean [glycine max (l.) merr.] genotypes with different susceptibility (susceptible 'spencer' and partially resistant 'pi567.374') to the disease sudden death syndrome (sds) caused by the soil-borne fungal pathogen fusarium solani f. sp. glycines (fsg) to study the role of isoflavonoids in the plant response to fsg infection. hairy root cultures obtained by transformation with agrobacterium rhizogenes allows normal root growth that can be visually monitored. ... | 2004 | 15331097 |
| intracranial fusariosis: a novel cause of fungal meningoencephalitis in a dog. | the fusarium species are a group of saprophytic fungal organisms that are occasionally the cause of opportunistic infections in humans and animals. central nervous system disease associated with a fusarium species is most commonly described in horse, resulting in equine leukoencephalomalacia. this report describes a 2-year-old, spayed, female german shepherd dog with meningoencephalitis secondary to infection with fusarium spp. meningoencephalitis in dogs secondary to a species of fusarium has n ... | 2004 | 15347824 |
| a non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean. | a non-specific lipid transfer peptide (nsltp) with antimicrobial activity was isolated from the mung bean (phaseolus mungo) seeds. the procedure entailed aqueous extraction, ion exchange chromatography on cm-sephadex and high performance liquid chromatography (hplc) on poros-hs-20. the peptide exhibited a molecular mass of 9.03 kda in mass spectrometry. it exerted antifungal action toward fusarium solani, fusarium oxysporum, pythium aphanidermatum and sclerotium rolfsii, and antibacterial action ... | 2004 | 15350690 |
| antibacterial and antifugal mono- and di-substituted symmetrical and unsymmetrical triazine-derived schiff-bases and their transition metal complexes. | a new series of antibacterial and antifungal triazine-derived mono- and di-substituted (symmetrical and unsymmetrical) schiff-bases and their cobalt(ii), copper(ii), nickel(ii) and zinc(ii) metal complexes have been synthesized and characterized by their elemental analyses, molar conductances, magnetic moments and ir and electronic spectral measurements. ir spectra indicated the ligands to act as tridentate towards divalent metal ions via a trazine-n, the azomethine-n and, indole-nh and deproton ... | 2004 | 15449731 |
| production in vitro of toxic macromolecules by strains of beauveria bassiana, and purification of a chitosanase-like protein secreted by a melanizing isolate. | the production of macromolecular insecticidal toxins in adamek's medium by two selected strains of beauveria bassiana was investigated. filtrates and dialysates of the melanizing strain 618 were toxic when injected into the lepidopteran insect galleria mellonella. separation by deae chromatography revealed that peaks eluted respectively with 100 and 200 mm nacl (p 100 and p 200) had an insecticidal activity and induced cuticular blackening. a hydrophilic protein, bclp, which causes the formation ... | 2004 | 15450862 |
| genotyping of 44 isolates of fusarium solani, the main agent of fungal keratitis in brazil. | in a retrospective study performed over 6 years in brazil, fusarium solani was found to be the most common species causing mycotic keratitis. the genetic diversity of 44 isolates from 39 patients was assessed by enterobacterial repetitive intergenic consensus pcr (eric-pcr) and pcr restriction fragment length polymorphism (pcr-rflp) fingerprinting. eric-pcr was more discriminatory than pcr-rflp for differentiating the strains. by combining of the results of both techniques, we identified 40 geno ... | 2004 | 15472299 |
| effectiveness of contact lens disinfectants after lens storage. | to determine the effectiveness of multipurpose contact lens solutions after incubation with lenses for one week. | 2004 | 15499238 |
| chemical interactions of brachiaria plantaginea with commelina bengalensis and acanthospermum hispidum in soybean cropping systems. | previous results obtained in soybean-wheat rotations under no-tillage conditions showed reductions in the seedbank of the weed species commelina benghalensis, but no alteration in the seedbank of acanthospermum hispidum in areas infested with brachiaria plantaginea. analyses of the soluble fraction of b. plantaginea indicated the predominance of aconitic acid (aa) among the aliphatic acids and ferulic acid (fa) among the phenolic acids. laboratory bioassays using c. benghalensis and a. hispidum ... | 2004 | 15503532 |
| in vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in china. | the antifungal susceptibility of isolates from chinese dermatomycosis patients to amorolfine was investigated following national committee for clinical laboratory standards (nccls) protocols. in total, 383 isolates were tested, including 132 strains from tinea pedis, 148 strains from tinea corporis/cruris, and 103 strains from onychomycosis. the minimum inhibitory concentration (mic) of amorolfine against dermatophytes ranged from 0.01 to 0.08 microg ml(-1). the mic(50) and mic(90) of amorolfine ... | 2004 | 15504124 |
| preliminary evidence of the role of hydrogen peroxide in the degradation of benzo[a]pyrene by a non-white rot fungus fusarium solani. | in order to study the enzymatic mechanisms involved in the successive steps of bap degradation by a deuteromycete fungus fusarium solani, we developed an indirect approach by using inhibitors of enzymes. we used either specific inhibitors of peroxidases (i.e. salicylhydroxamic acid) and of cytochrome p-450 (i.e. piperonyl butoxyde) or inhibitors of both enzymes (i.e. potassium cyanide). surprisingly, no expected decrease of bap degradation was observed with most inhibitors tested. on the contrar ... | 2004 | 14749063 |
| effect of immobilization support, water activity, and enzyme ionization state on cutinase activity and enantioselectivity in organic media. | we studied the reaction between vinyl butyrate and 2-phenyl-1-propanol in acetonitrile catalyzed by fusarium solani pisi cutinase immobilized on zeolites naa and nay and on accurel pa-6. the choice of 2-phenyl-1-propanol was based on modeling studies that suggested moderate cutinase enantioselectivity towards this substrate. with all the supports, initial rates of transesterification were higher at a water activity (a(w)) of 0.2 than at a(w) = 0.7, and the reverse was true for initial rates of h ... | 2004 | 14755562 |
| a promoter from pea gene drr206 is suitable to regulate an elicitor-coding gene and develop disease resistance. | abstract plant nonhost disease resistance is characterized by the induction of multiple defense genes. the pea drr206 gene is induced following inoculation with pathogens and treatment with abiotic agents, and moderately induced by wounding. a deletion series of drr206 promoter segments was fused with the beta-glucuronidase (gus) reporter gene and transiently transferred to tobacco, potato, and pea. gus activity revealed that two upstream regions of the drr206 promoter were particularly importan ... | 2004 | 18943490 |
| polycyclic aromatic hydrocarbons storage by fusarium solani in intracellular lipid vesicles. | accumulation and elimination of polycyclic aromatic hydrocarbons (pahs) were studied in the fungus fusarium solani. when the fungus was grown on a synthetic medium containing benzo[a]pyrene, hyphae of f. solani contained numerous lipid vesicles which could be stained by the lipid-specific dyes: sudan iii and rhodamine b. the fluorescence produced by rhodamine b and pah benzo[a]pyrene were at the same locations in the fungal hyphae, indicating that f. solani stored pah in pre-existing lipid vesic ... | 2005 | 15519459 |
| clinical experience in managing fusarium solani keratitis. | fusarium solani keratitis is a rare ocular infectious disease. the clinical characteristics and treatment methods of 18 patients with culture proven f. solani keratitis between july 1997 and december 2003 and with a follow-up period of more than 4 months were analysed retrospectively. the patients were divided into two groups based on the severity of keratitis. group a (n = 13) displayed non-severe keratitis and were treated with debridement, lamellar keratectomy and antifungal medication. group ... | 2005 | 15857351 |
| pathogen-induced expression of a cecropin a-melittin antimicrobial peptide gene confers antifungal resistance in transgenic tobacco. | expression of defensive genes from a promoter that is specifically activated in response to pathogen invasion is highly desirable for engineering disease-resistant plants. a plant transformation vector was constructed with transcriptional fusion between the pathogen-responsive win3.12t promoter from poplar and the gene encoding the novel cecropin a-melittin hybrid peptide (cema) with strong antimicrobial activity. this promoter-transgene combination was evaluated in transgenic tobacco (nicotiana ... | 2005 | 15863447 |
| real-time detection of common microbial volatile organic compounds from medically important fungi by selected ion flow tube-mass spectrometry (sift-ms). | we describe a new method, selected ion flow tube-mass spectrometry (sift-ms) for the rapid and sensitive real-time detection and quantification of volatile organic compounds from medically important fungi, grown on a range of laboratory media. sift-ms utilises the chemical ionisation reactions of mass-selected ions to characterise volatile organic compounds (vocs) that are produced as metabolites from fungi. this technique has the distinct advantage over others in that it readily detects low mol ... | 2005 | 15893831 |
| organometallic-based antibacterial and antifungal compounds: transition metal complexes of 1,1'-diacetylferrocene-derived thiocarbohydrazone, carbohydrazone, thiosemicarbazone and semicarbazone. | organometallic-based, 1,1'-diacetylferrocene-derived antibacterial and antifungal thiocarbohydrazone, carbohydrazone, thiosemicarbazone and semicarbazone have been prepared by condensing equimolar amount of 1,1'-diacetylferrocene with thiocarbohydrazide, carbohydrazide thiosemicarbazide and semicarbazide, respectively. these were used as ligands for the preparation of their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) metal complexes. all the synthesized ligands and their complexes were c ... | 2005 | 15895689 |
| removal of n-hexane by fusarium solani with a gas-phase biofilter. | a gas-phase biofilter inoculated with the fungus fusarium solani, isolated from a consortium grown on hexane vapors, was used to degrade this compound. the biofilter, packed with perlite and operated with an empty bed residence time of 60 s, was supplied with hexane concentrations between 0.5 gm(-3) and 11 gm(-3). biofilter performance was evaluated over 100 days of operation. several strategies for supplying the nutritive mineral medium were assayed to maintain favorable conditions for the fung ... | 2005 | 15933872 |
| effects of interleukin-15 on antifungal responses of human polymorphonuclear leukocytes against fusarium spp. and scedosporium spp. | fusarium spp. and scedosporium spp. have emerged as important fungal pathogens that are frequently resistant to antifungal compounds. we investigated the effects of human interleukin-15 (il-15) on human polymorphonuclear leukocyte (pmnl) activity against fusarium solani and fusarium oxysporum as well as scedosporium prolificans and scedosporium apiospermum. il-15 (100 ng/ml) significantly enhanced pmnl-induced hyphal damage of both fusarium spp. and s. prolificans after incubation for 22 h (p < ... | 2005 | 15935692 |
| assessing the pathogenic effect of fusarium, geosmithia and ophiostoma fungi from broad-leaved trees. | phytopathogenic effect of geosmithia pallida, g. langdonii, ophiostoma grandicarpum, o. querci, two isolates of o. piceae, and two isolates of fusarium solani was compared using plant growth test (stem and root length of garden cress plants seeded on mycelium-covered potato carrot agar); ophiostoma spp. and f. solani were isolated from oak, geosmithia spp. from galleries of scolytus intricatus on beech. all fungi inhibited more the root elongation than that of stems. f. solani led to plant colla ... | 2005 | 15954534 |
| in-vitro antibacterial, antifungal and cytotoxic properties of sulfonamide--derived schiff's bases and their metal complexes. | a series of new antibacterial and antifungal schiff's bases derived from sulfonamides, as well as their transition metal complexes incorporating cobalt (ii), copper (ii), nickel (ii) and zinc (ii) were synthesized, characterized and screened for their in-vitro antibacterial activity against six gram-negative (escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi and shigella dysentriae) and four gram-positive (bacillus cereus, corynebacterium diphth ... | 2005 | 15968823 |
| recurrent colonization of successively implanted tracheoesophageal vocal prostheses by a member of the fusarium solani species complex. | tracheoesophageal vocal prostheses (tvp) in laryngectomized patients commonly deteriorate due to overgrowth by yeasts, particularly candida species. we describe the first case of colonization of such devices by a member of the fusarium solani species complex in a patient with a history of glottal carcinoma. three isolates, from three prostheses, were found morphologically consistent with the traditional picture of f. solani. ribosomal sequence analysis showed that the isolates belonged to a dist ... | 2005 | 15695678 |
| concordant evolution of trichothecene 3-o-acetyltransferase and an rdna species phylogeny of trichothecene-producing and non-producing fusaria and other ascomycetous fungi. | the cereal pathogen fusarium graminearum species complex (e.g. fusarium asiaticum, previously referred to as f. graminearum lineage 6) produces the mycotoxin trichothecene in infected grains. the fungus has a gene for self-defence, tri101, which is responsible for 3-o-acetylation of the trichothecene skeleton in the biosynthetic pathway. recently, trichothecene non-producers fusarium oxysporum and fusarium fujikuroi (teleomorph gibberella fujikuroi) were shown to have both functional (tri201) an ... | 2005 | 15699200 |
| a generic system for the escherichia coli cell-surface display of lipolytic enzymes. | esta is an outer membrane-anchored esterase from pseudomonas aeruginosa. an inactive esta variant was used as an anchoring motif for the escherichia coli cell-surface display of lipolytic enzymes. flow cytometry analysis and measurement of lipase activity revealed that bacillus subtilis lipase lipa, fusarium solani pisi cutinase and one of the largest lipases presently known, namely serratia marcescens lipase were all efficiently exported by the esta autotransporter and also retained their lipol ... | 2005 | 15710409 |
| genomic analysis of a region encompassing qrfs1 and qrfs2: genes that underlie soybean resistance to sudden death syndrome. | candidate genes were identified for two loci, qrfs2 providing resistance to the leaf scorch called soybean (glycine max (l.) merr.) sudden death syndrome (sds) and qrfs1 providing resistance to root infection by the causal pathogen fusarium solani f.sp. glycines. the 7.5 +/- 0.5 cm region of chromosome 18 (linkage group g) was shown to encompass a cluster of resistance loci using recombination events from 4 near-isogenic line populations and 9 dna markers. the dna markers anchored 9 physical map ... | 2005 | 15729404 |
| solar and photocatalytic disinfection of protozoan, fungal and bacterial microbes in drinking water. | the ability of solar disinfection (sodis) and solar photocatalytic (tio(2)) disinfection (spc-dis) batch-process reactors to inactivate waterborne protozoan, fungal and bacterial microbes was evaluated. after 8 h simulated solar exposure (870 w/m(2) in the 300 nm-10 microm range, 200 w/m(2) in the 300-400 nm uv range), both spc-dis and sodis achieved at least a 4 log unit reduction in viability against protozoa (the trophozoite stage of acanthamoeba polyphaga), fungi (candida albicans, fusarium ... | 2005 | 15743634 |
| unfolding and inactivation of cutinases by aot and guanidine hydrochloride. | we present a comparative analysis of the unfolding and inactivation of three cutinases in the presence of guanidine hydrochloride (gdnhcl) and bis(2-ethylhexyl) sodium sulfosuccinate (aot). previous investigations have focused on the cutinase from fusarium solani pisi (fsc). in addition to fsc, the present study includes the cutinase from humicola insolens (hic) and a mutant variant of hic (muhic) with increased activity and decreased surfactant sensitivity. equilibrium and time-resolved denatur ... | 2005 | 15752695 |
| root response to fusarium solani f. sp . glycines: temporal accumulation of transcripts in partially resistant and susceptible soybean. | sudden death syndrome (sds) of soybean is a complex of root rot disease caused by the semi-biotrophic fungus fusarium solani f. sp. glycines (fsg) and a leaf scorch disease caused by toxins produced by the pathogen in the roots. development of partial rate-reducing resistance in roots to sds was studied. the recombinant inbred line 23 (ril23) that carried resistance conferred by six quantitative trait loci (qtl) derived from cultivars 'essex' x 'forrest' was compared to the susceptible cultivar ... | 2005 | 15815926 |
| a chitinase with antifungal activity from the mung bean. | a chitinase with antifungal activity was isolated from mung bean (phaseolus mungo) seeds. the procedure entailed aqueous extraction, (nh4)2so4 precipitation, ion-exchange chromatography on cm-sepharose, high-performance liquid chromatography (hplc) on poros hs-20, and gel filtration on sephadex g-75. the protein exhibited a molecular mass of 30.8 kda in sds-polyacrylamide gel electrophoresis. its pi was 6.3 as determined by isoelectric focusing. the specific activity of the chitinase was estimat ... | 2005 | 15766863 |
| first report of a novel plant lysozyme with both antifungal and antibacterial activities. | a novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4kda in sds-polyacrylamide gel electrophoresis was isolated from mung bean (phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on cm-sephadex, and high-performance liquid chromatography on poros hs-20. its n-terminal sequence was very different from that of hen egg white lysozyme. its pi was estimated to be above 9.7. the specific act ... | 2005 | 15649419 |
| chemical composition and antifungal activity of essential oils from different tissues of japanese cedar (cryptomeria japonica). | in this study antifungal activities of essential oils from different tissues of japanese cedar (cryptomeria japonica d. don) against four wood decay fungi and six tree pathogenic fungi were investigated. in addition, the yields of essential oils obtained by water distillation were compared and their constituents determined by gc-ms analyses. the yield of essential oils from four tissues of japanese cedar is in the decreasing order of leaf (27.38 ml/kg) > bark (6.31 ml/kg) > heartwood (3.80 ml/kg ... | 2005 | 15686410 |
| effect of voriconazole combined with micafungin against candida, aspergillus, and scedosporium spp. and fusarium solani. | effects of voriconazole combined with micafungin against 101 isolates of candida spp. and 100 isolates of filamentous fungi have been evaluated by in vitro checkerboard analysis. the combination was indifferent for 97% of the candida isolates and synergistic for 64% of the filamentous fungi (79% for aspergillus fumigatus). | 2005 | 16304192 |
| methyl tert-butyl ether and tert-butyl alcohol degradation by fusarium solani. | fusarium solani degraded methyl tert-butyl ether (mtbe) and other oxygenated compounds from gasoline including tert-butyl alcohol (tba). the maximum degradation rate of mtbe was 16 mg protein h and 46 mg/g protein h for tba. the culture transformed 77% of the total carbon to 14co2. the estimated yield for mtbe was 0.18 g dry wt/g mtbe. | 2005 | 16314973 |
| synthesis and evaluation of demethoxyviridin derivatives as potential antimicrobials. | the in vitro antibacterial and antifungal activities of demethoxyviridin and some synthetic analogues were evaluated by the agar diffusion method. the minimum inhibitory concentrations (mic) of the active compounds were also determined by the agar dilution method. demethoxyviridin (1) showed moderate antibacterial activity against most of the strains tested. 1alpha-hydroxydemethoxyviridin (3) showed antibacterial activity and the most potent in vitro antifungal activity with mic of 20 microg/ml ... | 2005 | 16320609 |
| substrate specificity and kinetic properties of enzymes belonging to the hormone-sensitive lipase family: comparison with non-lipolytic and lipolytic carboxylesterases. | we have studied the kinetics of hydrolysis of triacylglycerols, vinyl esters and p-nitrophenyl butyrate by four carboxylesterases of the hsl family, namely recombinant human hormone-sensitive lipase (hsl), est2 from alicyclobacillus acidocaldarius, afest from archeoglobus fulgidus, and protein rv1399c from mycobacterium tuberculosis. the kinetic properties of enzymes of the hsl family have been compared to those of a series of lipolytic and non-lipolytic carboxylesterases including human pancrea ... | 2005 | 16325466 |
| fusarium solani breast abscess. | an unusual manifestation of breast fusariosis was encountered in a 55-year-old female diabetic patient. two fine needle aspirates (fna) from the abscess were done at three days interval and they showed hyaline, septate, branched, fungal hyphae in 10% potassium hydroxide mount. fungal infection was confirmed by demonstrating the fungal hyphae in the midst of lymphocytes, macrophages and neutrophils in leishman stained smears. culture of both fnas yielded a heavy and pure growth of fusarium solani ... | 2005 | 16100431 |
| roles of adherence and matrix metalloproteinases in growth patterns of fungal pathogens in cornea. | to investigate the roles of adherence and matrix metalloproteinases (mmps) in growth patterns of major fungal pathogens in cornea. | 2005 | 16109640 |
| quantization of ph: evidence for acidic activity of triglyceride lipases. | here we present a study of lipolytic activity of lipases from fusarium solani pisi (cutinase), rhizomucor miehei, pseudomonas cepacia, and humicola lanuginosa. their activities toward triolein provide clear evidence for considerable enzymatic activity under acidic conditions. the activity was followed using fourier transform infrared attenuated total reflection (ftir-atr) and nuclear magnetic resonance (nmr). using these approaches, all the lipases that were studied exhibited lipolytic activity ... | 2005 | 16114894 |
| enzymatic surface modification of poly(ethylene terephthalate). | this study unambiguously confirms hydrolysis using cutinase of the persistent synthetic polymer poly(ethylene terephthalate), the most important synthetic fiber in the textile industry by direct measurement and identification of the different hydrolysis products. in this aqueous heterogeneous system, dissolved cutinase from fusarium solani pisi acts on different solid poly(ethylene terephthalate) substrates. the extent of hydrolysis was detected by measuring the amount of (soluble) degradation p ... | 2005 | 16115695 |
| biological activity of spatoglossum asperum: a brown alga. | chloroform and methanol fractions of an ethanol extract of spatoglossum asperum showed antifungal activity against the highly destructive plant pathogen, macrophomina phaseolina while the n-hexane fraction showed activity against rhizoctonia solani and fusarium solani. n-hexane and methanol fractions also showed nematicidal activity against the plant parasitic nematode, meloidogyne javanica. column chromatography of the ethanol extract of seaweed yielded oily fractions named sa-i to sa-vi. gc-ms ... | 2005 | 16161033 |
| fungal keratitis due to fusarium solani. | a young man presented with swelling, congestion and lacrimation of right eye for one week. on examination, he had corneal ulcer. corneal scrapings on lactophenol cotton blue stain revealed fungal hyphae. culture on sabouraud agar yielded fusarium solani after 48 hours. patient was treated with ketoconazole and miconazole and responded well. | 2005 | 16181584 |
| in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes. | a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ... | 2005 | 16206827 |
| quantitative assessment of phytopathogenic fungi in various substrates using a dna macroarray. | detection, identification and quantification of plant pathogens are the cornerstones of preventive plant disease management. to detect multiple pathogens in a single assay, dna array technology currently is the most suitable technique. however, for sensitive detection, polymerase chain reaction (pcr) amplification before array hybridization is required. to evaluate whether dna array technology can be used to simultaneously detect and quantify multiple pathogens, a dna macroarray was designed and ... | 2005 | 16232285 |
| evaluation of fusarium solani hyphae and conidia susceptibility to amphotericin b and itraconazole: study of a clinical case. | fusarium species are hyaline moulds belonging to the hyalohyphomycosis group that are usually found in the soil and plants. this organism has emerged as a cause of disseminated invasive disease. the correlation between in vitro value and clinical efficacy is low and many patients remain unresponsive to treatment despite in vitro susceptibility. we determined growth control for fusarium solani using the biocell-tracer system that measures the growth rate of a single fungal hypha, and the effect o ... | 2005 | 16244897 |
| analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis. | a comparison of amino acid sequences of fungal chitosanases, belonging to family 75 of glycosyl hydrolases, revealed three carboxylic amino acid residues completely conserved among all of the chitosanases. to study the role of these residues in catalysis, they were replaced with other residues by site-directed mutagenesis in the chitosanase gene of fusarium solani. the mutated genes were expressed in the yeast saccharomyces cerevisiae and the resulting recombinant chitosanases were used in kinet ... | 2005 | 16384794 |
| the causal agents of damping-off disease of buglosse from iran. | iran is considered a major genetic for medicinal plant in the world. because of this significant diversity and historical background in identification and utilization to remedy human and animal diseases, export of medicinal plant can help to strengthen local as well as natural economy. buglosse (fig. 1) is one of the most important and common medicinal plants in iran and exist as echium amoneum and borago officinalis. this work was conducted in order to identify the causal agent(s) of damping of ... | 2005 | 16637194 |
| reaction of selected soybean cultivars to rhizoctonia root rot and other damping-off disease agents. | eight soybean cultivars; giza 21. giza 22, giza 35, giza 82, giza 83, crawford, holladay and toamo were evaluated to rhizoctonia root rot using agar plate and potted plant techniques. data cleared that, in agar plate assay all soybean cultivars were moderately susceptible (ms), although the differences between them were significant (p=0.05). generally, in potted assay, the reactions were resistant (r) or moderately resistant (mr) to root rots. also, the differences between cultivars were signifi ... | 2005 | 16637203 |
| variability of non-mutualistic filamentous fungi associated with atta sexdens rubropilosa nests. | a survey of the filamentous fungi other than the symbiotic one found in association with atta sexdens rubropilosa colonies was carried out. different fungal species (27 taxa) were isolated a few days after treating the workers with toxic baits (sulfluramid; mirex-s), from 40 laboratory and 20 field nests. syncephalastrum racemosum (54%) and escovopsis weberi (21%), trichoderma harzianum (38%) and fusarium oxysporum (23%) were the prevalent species in laboratory and field nests, respectively. acr ... | 2005 | 16475502 |
| fragrance release from the surface of branched poly (amide)s. | enzymes are powerful tools in organic synthesis that are able to catalyse a wide variety of selective chemical transformations under mild and environmentally friendly conditions. enzymes such as the lipases have also found applications in the synthesis and degradation of polymeric materials. however, the use of these natural catalysts in the synthesis and the post-synthetic modification of dendrimers and hyperbranched molecules is an application of chemistry yet to be explored extensively. in th ... | 2005 | 18007278 |
| cloning and sequence analysis of ornithine decarboxylase gene fragments from the ascomycota. | ornithine decarboxylase (odc; ec 4.1.1.17) catalyzes the initial step in the biosynthesis of polyamines, the conversion of ornithine to putrescine. based on the most conserved regions of fungal odcs, we designed and synthesized oligonucleotides to amplify homologous fragments of three important plant pathogenic pyrenomycete fungi (ascomycota), magnaporthe grisea, colletotrichum lindemuthianum and fusarium solani, and one insect pathogenic fungus metarhizium anisopliae. cloning and sequencing of ... | 2006 | 17286052 |
| head-to-head comparison of the activities of currently available antifungal agents against 3,378 spanish clinical isolates of yeasts and filamentous fungi. | we have compared the activities of posaconazole and other currently available antifungal agents against a collection of 3,378 clinical isolates of yeasts and filamentous fungi. a total of 1,997 clinical isolates of candida spp., 359 of other yeast species, 697 strains of aspergillus spp., and 325 nondermatophyte non-aspergillus spp. were included. the average geometric means of the mics of agents that were tested against candida spp. were 0.23 microg/ml for amphotericin b, 0.29 microg/ml for flu ... | 2006 | 16495251 |
| systemic fungemia and hepatic localizations of fusarium solani in a liver transplanted patient: an emerging fungal agent. | the incidence of invasive fungal infection is increasing especially in the field of transplantation, affecting as many as 50% of bone marrow transplant (bmt) patients with neutropenia and 5-20% of solid-organ transplant (sot) recipients. fusarium species are soil saprophytes and plant pathogens. they may cause superficial mycoses or important opportunistic infections in patients with bone marrow suppression and neutropenia, they have been rarely described in solid organ recipients, and up to now ... | 2006 | 17058254 |
| report on testing from an investigation of fusarium keratitis in contact lens wearers. | recent reports of disproportionate outbreaks of microbial keratitis caused by fusarium solani have led to the removal of the bausch & lomb multipurpose lens care solution renu with moistureloc ("moistureloc") from the global market. previous studies have shown an increased risk of contact lens-related corneal infection in connection with overnight wear, noncompliant lens wearing and lens care behaviors, specific treatment regimens, and other patient-related and non-patient-related factors. the p ... | 2006 | 17099384 |
| biocide uptake in contact lenses and loss of fungicidal activity during storage of contact lenses. | with recent outbreaks of fusarium keratitis related to contact lens wear, studies were conducted to determine the biocide uptake during lens storage, and the resulting effect on antifungal activity of related products. | 2006 | 17099385 |
| cladosporium fulvum avr4 protects fungal cell walls against hydrolysis by plant chitinases accumulating during infection. | resistance against the leaf mold fungus cladosporium fulvum is mediated by the tomato cf proteins which belong to the class of receptor-like proteins and indirectly recognize extracellular avirulence proteins (avrs) of the fungus. apart from triggering disease resistance, avrs are believed to play a role in pathogenicity or virulence of c. fulvum. here, we report on the avirulence protein avr4, which is a chitin-binding lectin containing an invertebrate chitin-binding domain (cbm14). this domain ... | 2006 | 17153926 |
| development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a bac minimum tile path. | genome analysis of soybean (glycine max l.) has been complicated by its paleo-autopolyploid nature and conserved homeologous regions. landmarks of expressed sequence tags (ests) located within a minimum tile path (mtp) of contiguous (contig) bacterial artificial chromosome (bac) clones or radiation hybrid set can identify stress and defense related gene rich regions in the genome. a physical map of about 2,800 contigs and mtps of 8,064 bac clones encompass the soybean genome. that genome is bein ... | 2006 | 17156445 |
| growth and survival of fusarium solani-f. oxysporum complex on stressed multipurpose contact lens care solution films on plastic surfaces in situ and in vitro. | to analyze factors implicating the association of renu with moistureloc (renu ml) multipurpose contact lens solution (mps) with the increased incidence of fusarium keratitis. | 2006 | 17172900 |
| secretion of keratinolytic enzymes and keratinolysis by scopulariopsis brevicaulis and trichophyton mentagrophytes: regression analysis. | a survey on keratinophilic fungi from poultry-farm soils at namakkal and from feather dumping soils at chennai, india, revealed the existence of 34 species of fungi. most of the fungi exhibited variable efficiency in producing extracellular keratinase when grown in plates with chicken feathers as the sole carbon and nitrogen source. the fungi aspergillus flavus, aspergillus niger, aspergillus versicolor, chrysosporium state of arthroderma tuberculatum, paecilomyces carneus, scopulariopsis brevic ... | 2006 | 17215897 |
| in-vitro antibacterial, antifungal and cytotoxic activity of cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes with furanylmethyl- and thienylmethyl-dithiolenes: [1, 3-dithiole- 2-one and 1,3-dithiole-2-thione]. | some antibacterial and antifungal furanylmethyl-and thienylmethyl dithiolenes and, their co(ii), cu(ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against four gram-negative; escherichia coli, pseudomonas aeruginosa, salmonella typhi and shigella flexeneri, and two gram-positive; bacillus subtilis and staphylococcus aureus bacterial strains, and for in-vitro antifungal activity against trichophyton longifusus, candid ... | 2006 | 17252947 |
| in-vitro antibacterial, antifungal and cytotoxic properties of metal-based furanyl derived sulfonamides. | a new series of antibacterial and antifungal furanyl-derived sulfonamides and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) metal complexes have been synthesized, characterized and screened for their in-vitro antibacterial activity against four gram-negative (escherichia coli, shigella flexneri, pseudomonas aeruginosa and salmonella typhi) and two gram-positive (bacillus subtilis and staphylococcus aureus) bacterial strains and, for in-vitro antifungal activity against trichophyton l ... | 2006 | 17252952 |
| [bilateral proximal cellulitis and onychomycosis in both big toes due to fusarium solani]. | we report a case of proximal fold cellulitis in both big toes, associated with a bilateral proximal onychomycosis and an intertrigo of the fourth space due to fusarium solani. the infection occurred in an immunocompetent man with diabetes mellitus type ii. apparently, the infection was acquired in a tropical country and once the patient was in spain the infection progressed causing nail detachment (onychomadesis). seven months later a relapse that affected the left toenail occurred. the patient ... | 2006 | 17388651 |
| chemical composition and antifungal activity of the essential oils of three species of artemisia on some soil-borne phytopathogens. | various species of the genus artemisia are used for their pharmacological, antimicrobial, antioxidant activity. three species of this genus, artemisia scoparia, a. sieberi and a. aucheri are widely distributed in desert area of iran. in order to identify the chemical composition, aerial parts of a. scoparia, a. sieberi and a. aucheri were collected from bajestan (khorasan province) at flowering stage. the essential oils were obtained by hydro-distillation of air-dried samples and their chemical ... | 2006 | 17390897 |
| metal-based antibacterial and antifungal agents: synthesis, characterization, and in vitro biological evaluation of co(ii), cu(ii), ni(ii), and zn(ii) complexes with amino acid-derived compounds. | a series of antibacterial and antifungal amino acid-derived compounds and their cobalt(ii), copper(ii), nickel(ii), and zinc(ii) metal complexes have been synthesized and characterized by their elemental analyses, molar conductances, magnetic moments, and ir, and electronic spectral measurements. ligands (l(1))-(l(5)) were derived by condensation of beta-diketones with glycine, phenylalanine, valine, and histidine and act as bidentate towards metal ions (cobalt, copper, nickel, and zinc) via the ... | 2006 | 17497020 |
| gaseous hexane biodegradation by fusarium solani in two liquid phase packed-bed and stirred-tank bioreactors. | biofiltration of hydrophobic volatile pollutants is intrinsically limited by poor transfer of the pollutants from the gaseous to the liquid biotic phase, where biodegradation occurs. this study was conducted to evaluate the potential of silicone oil for enhancing the transport and subsequent biodegradation of hexane by the fungus fusarium solani in various bioreactor configurations. silicone oil was first selected among various solvents for its biocompatibility, nonbiodegradability, and good par ... | 2006 | 16646479 |
| antimicrobial activity of potato aspartic proteases (staps) involves membrane permeabilization. | solanum tuberosum aspartic proteases (staps) with antimicrobial activity are induced after abiotic and biotic stress. in this study the ability of staps to produce a direct antimicrobial effect was investigated. viability assays demonstrated that staps are able to kill spores of fusarium solani and phytophthora infestans in a dose-dependent manner. localization experiments with fitc-labelled staps proved that the proteins interact directly with the surface of spores and hyphae of f. solani and p ... | 2006 | 16804179 |
| molecular characterization of a potato map kinase transcriptionally regulated by multiple environmental stresses. | the mapk cascade is an evolutionary conserved signaling pathway that links external stimuli with cellular responses. using polymerase chain reaction (pcr), a dna fragment corresponding to a solanum tuberosum mapk, stmpk1, was isolated. stmpk1 amino acid sequence displayed over 90% identity with tomato mpk1 (lempk1) and tobacco sipk. southern blot analysis indicated that the gene encoding stmpk1 is present in a single copy in the potato genome. stmpk1 mrna levels differentially accumulated in pot ... | 2006 | 16814558 |
| light is essential for degradation of ribulose-1,5-bisphosphate carboxylase-oxygenase large subunit during sudden death syndrome development in soybean. | fusarium solani f. sp. glycines (fsg) has been reported to produce at least two phytotoxins. cell-free fsg culture filtrates containing phytotoxins have been shown to develop foliar sudden death syndrome (sds) in soybean. we have investigated the changes in protein profiles of diseased leaves caused by cell-free fsg culture filtrates prepared from fsg isolates. two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) was conducted to investigate the protein profiles of di ... | 2006 | 16821191 |
| nutritive value, antioxidant and antimicrobial properties of struchium sparganophora leaves. | struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in nigeria. fresh leaves of s. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried s. sparganophora leaves were extracted with rectified spirit. the antioxidant and antimicrobial properties of the extracts were subsequently determined. the result of the study revealed that the fresh ... | 2006 | 16822216 |
| fsfks1, the 1,3-beta-glucan synthase from the caspofungin-resistant fungus fusarium solani. | the cell wall, a mesh of carbohydrates and proteins, shapes and protects the fungal cell. the enzyme responsible for the synthesis of one of the main components of the fungal wall, 1,3-beta-glucan synthase, is targeted by the antifungal caspofungin acetate (cfa). clinical isolates of candida albicans and aspergillus fumigatus are much more sensitive to cfa than clinical isolates of fusarium species. to better understand cfa resistance in fusarium species, we cloned and sequenced fsfks1, which en ... | 2006 | 16835448 |