Publications

genetics of biosynthesis and overproduction of penicillin.		1976	823642
[action of various beta(1-3)-d-glucanases on the wall of yeasts: taxonomic applications].	purified beta-(1-3)-d-glucanases (from aspergillus nidulans, badidiomycetes sp. qm 806, trichoderma viride) are used to release protoplasts from various yeasts. two of them may prove taxonomic correlation. the enzyme of basidomycetes sp. releases protoplasts from ascomycetes, the enzyme of t. viride releases protoplasts from ascomycetes and heterobasidiomycetes; none of them acts on the basidiomycetes.	1976	826331
role of nicotinic acid in pyridoxine biosynthesis in aspergillus nidulans.		1976	999690
the reconstitution of anacystis nidulans dna-dependent rna polymerase from its isolated subunits.	the dna-dependent rna polymerase of the blud-green alga anacystis nidulans was reconstituted from its isolated subunits in the absence of urea. applying this technique the kinetics and the subunit requirements of the reconstitution process were analyzed. the results reveal differences with respect to the reconstitution of escherichia coli polymerase. reconstitution proceeds much more slowly in the case of the a. nidulans enzyme. reconstitution here is absolutely dependent on the presence of the ...	1976	814000
chloral hydrate induced haploidization in aspergillus nidulans.	this is the first report of induction of haploidization in aspergillus nidulans by chloral hydrate, which is an efficient polyploidizing agent for higher plants and a psychotropic drug for man. a new procedure has been described to isolate haploids from diploids with a very high frequency, as compared to p-fluorophenylalanine, which is generally used for this purpose.	1976	786720
fungal infections after renal transplantation.	fungal infections occurred in 3 of 22 renal transplants (13.6%). two patients developed rhinocerebral phycomycosis, and rhizopus arrhizus was isolated from cultures of the nasal conchae. both patients were treated with systemic amphotericin b. three-and-a-half months after transplantation the third patient developed an acute ureteral obstruction secondary to infection with aspergillus nidulans; this necessitated removal of the graft. there was no immediate mortality associated with these fungal ...	1976	786944
the genetic instability and mutagenic interaction of chromosomal duplications present together in haploid strains of aspergillus nidulans.	previous work has shown that strains of aspergillus nidulans with a chromosome segment in duplicate (one in normal position, one translocated to another chromosome) are unstable. deletions occur from either duplicate segment. the present work has shown that when a chromosome i duplication and a chromosome iii duplication are together in a haploid, deletions from the intact iii duplication generally precede deletions from particular sections of the i duplication. furthermore, the iii duplication ...	1976	787776
tumor induction by a single subcutaneous injection of sterigmatocystin in newborn mice.	sterigmatocystin, a mycotoxin produced by aspergillus versicolor, aspergillus sydowi, aspergillus nidulans, and a species of bipolaris, was given to newborn balb/c x dba/2f1 (hereafter referred to as cd2f1) mice by a single s.c. administration in 1% gelatin suspension. in an acute toxicity study, the maximum tolerated dose of sterigmatocystin was 5 mug/g body weight. in a chronic study, a single s.c. injection of 5, 1, or 0.5 mug/g body weight gave rise to high incidences of lung and liver adeno ...	1976	1268822
cytochrome abnormalities and cyanide-resistant respiration in extranuclear mutants of aspergillus nidulans.	the cytochrome spectra of two extranuclear mutants of aspergillus nidulans and the double-mutant recombinant formed from them have been examined both at room temperature and at the temperature of liquid n2 and compared with those of the wild-type strain. the oligomycin-resistant, slow growing mutant contained an increased amount of cytochrome c without any loss of cytochromes b and a,a3. the cold-sensitive mutant, apparently normal when grown at 37 c, showed an increased amount of cytochrome c a ...	1976	1107321
polyamine and ornithine metabolism during the germination of conidia of aspergillus nidulans.	1. the activities of ornithine decarboxylase, s-adenosylmethionine decarboxylase and ornithine-2-oxoglutarate aminotransferase were studied during the first 24 h of conidial germination in aspergillus nidulans. 2. increases (over 100-fold) in the activities of ornithine decarboxylase and s-adenosylmethionine decarboxylase occurred during the emergence of the germ-tube and before the doubling of dna and this was followed by a sharp fall in the activities of both enzymes by 16h. 3. the increase in ...	1976	791270
[dna degradation induced by radiation in aspergillus nidulans].		1976	791383
phosphorus uptake in biotin deficient aspergillus nidulans: an evidence for the involvement of binding protein in this process.		1976	791857
relationship of the genetic determination of impaired penicillin production in naturally occurring strains to that in induced mutants of aspergillus nidulans.		1976	792389
estimation of the generation time and peripheral growth zone of aspergillus nidulans and alternaria solani hyphae from radial growth rates and ranges in apical cell length.		1976	792392
purification of a deoxyribonuclease from aspergillus nidulans.		1976	793906
mitosis, septation, branching and the duplication cycle in aspergillus nidulans.	mitosis, septation and branching were studied in undifferentiated mycelia and leading hyphae of aspergillus nidulans, a mould which forms incomplete septa. after spore germination, nuclei divided synchronously until germ-tube hyphae contained 8 or 16 nuclei; mitosis occurred when the volume of cytoplasm per haploid nucleus was about 60 mum3. intercompartment development was not synchronized, consequently mitosis in the mycelium as a whole eventually became asynchronous. during the stage of async ...	1976	796408
formation of nadph-nitrate reductase activity in vitro from aspergillus nidulans niad and cnx mutants.	mutants of a. nidulans at several loci lack detectable nadph-nitrate reductase activity. these loci include niad, the structural gene for the nitrate reductase polypeptide, and five other loci termed cnxabc, e, f, g and h which are presumed to be involved in the formation of a molybdenum-containing component (mcc) necessary for nitrate reductase activity. when forzen mycelia from a. nidulans deletion mutant niad26 were homogenized in a ten broeck homogenizer together with frozen mycelia from eit ...	1976	796678
panel discussion on submammalian systems.		1976	796705
synthesis of polyamines in the germinating conidia of aspergillus nidulans.		1976	798705
the effects of putrescine, spermidine, and spermine on the growth of a polyamine-requiring mutant of aspergillus nidulans.		1976	798706
presence of histones in aspergillus nidulans.	five major histone proteins have been extracted from chromatin isolated from purified nuclei of the fungus, aspergillus nidulans. these proteins had chromatographic properties which were similar to reference calf thymus histones and were purified to electrophoretic homegeneity by gel chromatography of bio-gel p10, bio-gel p60, and sephadex g-100. electrophoresis of these proteins in three different systems (urea-starch, urea-acetic acid polyacrylamide, and discontinuous sds polyacrylamide) showe ...	1976	799641
choline-o-sulphate utilization in aspergillus nidulans.		1976	800000
maternal inheritance of extranuclear mitochondrial markers in aspergillus nidulans.		1976	800001
cholorate toxicity in aspergillus nidulans: the selection and characterisation of chlorate resistant mutants.	mutation in at least ten genes can result in chlorate reistance in aspergillus nidulans. mutation in seven of these genes also results in the inability to use nitrate as nitrogen source. the various classes of resistant mutant obtained occur in different proportions, depending on whether or not a mutagenic treatment is employed, and also on which nitrogen source is used for selection. the prinicipal effect of mutagen arises because mutations in the niad gene, the nitrate reductase structural gen ...	1976	773908
well-marked strains of aspergillus for tests of environmental mutagens: identification of induced mitotic recombination and mutation.		1976	775325
mutants of aspergillus nidulans lacking pyruvate kinase.		1976	776698
the isolation of nuclei from the filamentous fungus aspergillus nidulans.	a method has been developed for isolating nuclei from the filamentous fungus aspergillus nidulans. in this procedure, the mycelia from 14 to 16 h spore-derived cultures of a. nidulans nri, a stable diploid strain, were frozen with liquid nitrogen and homogenized in a waring blender. after homogenization, the mycelia were warmed to 4 degrees c and the nuclei were purified from the homogenate by differential centrifugation followed by sedimentation through 2.1 m-sucrose. the final nuclear yield wa ...	1976	778331
effect of glucose, ammonium and media maintenance on the time of conidiophore initiation by surface colonies of aspergillus nidulans.		1976	778332
agar as a carbon source and its effect on the utilization of other carbon sources by acetate non-utilizing (acu) mutants of aspergillus nidulans.		1976	778333
integrator gene in aspergillus nidulans.		1976	778641
enhancement of tissue invasion in murine aspergillosis by systemic administration of suspensions of killed corynebacterium parvum.	the effect of killed corynebacterium parvum vaccine on the course of murine aspergillosis is described. a grid-counting technique was employed to quantitate tissue invasion by aspergillus nidulans in the brain, heart, and kidneys (the target organs) of normal mice and of mice treated systemically with killed c. parvum vaccine. simultaneous treatment of mice with c. parvum and a. nidulans significantly increased the mortality rate, in contrast to treatment of mice with c. parvum prior to or follo ...	1976	779492
proceedings: influence of microwaves on genetical processes of aspergillius nidulans.		1976	781259
partial purification of methionine binding protein from aspergillus nidulans by affinity chromatography.		1976	782531
nucleosome structure in aspergillus nidulans.	the structure of chromatin from aspergillus nidulans was studied using micrococcal nuclease and dnaase i. limited digestion with micrococcal nuclease revealed a nucleosomal repeat of 154 base pairs for aspergillus and 198 base pairs for rat liver. with more extensive digestion, both types of chromatin gave a similar quasi-limit product with a prominent fragment at 140 base pairs. the similarity of the two limit digests suggests that the structure of the 140 base pair nucleosome core is conserved ...	1976	782724
mitotic crossing over and nondisjunction in translocation heterozygotes of aspergillus.	to analyze mitotic recombination in translocation heterozygotes of a. nidulans two sets of well-marked diploids were constructed, homo- or heterozygous for the reciprocal translocations t1 (il;viir) or t2 (il;viir) and heterozygous for selective markers on il. it was found that from all translocation heterozygotes some of the expected mitotic crossover types could be selected. such crossovers are monosomic for one translocated segment and trisomic for the other and recovery depends on the relati ...	1976	773747
initial characterization of aspergillus nidulans mutants blocked in the nuclear replication cycle.	several hundred temperature-sensitive mutants of aspergillus nidulans were screened for ability of their conidia to produce germ tubes at the nonpermissive temperature while still remaining with the original single conidial nucleus.	1976	770457
determination of the execution points of mutations in the nuclear replication cycle of aspergillus nidulans.	cultures of nuclear replication cycle mutants of aspergillus nidulans were transferred to the nonpermissive temperature, and the fraction of nuclei still able to reach mitosis was determined. for the determinations, benomyl [methyl-1(butylcarbomoyl)benzimidazolecarbamate] was added to trap nuclei in mitosis, and these were detected by staining with aceto-orcein. the assumptions and controls required to relate the experimentally determined fractions to the points where a mutation blocks the nucle ...	1976	770458
tubulin-like protein from aspergillus nidulans.		1976	773373
the effects of temperature on genetic instability in aspergillus nidulans.	previous work has shown that strains of aspergillus nidulans with a chromosome segment in duplicate (one in normal position, one translocated to another chromosome) are unstable. deletions occur from either duplicate segment. the present work has shown that most deletions occur from the translocated duplicate segment. furthermore, it has been found that the overall frequency of deletions from a duplication is dependent upon the temperature of growth. the overall frequency of deletions from a chr ...	1976	765807
hyper-repressible operator-type mutant in sulphate permease gene of aspergillus nidulans.		1976	765839
a temperature-sensitive mutant of aspergillus nidulans reversibly blocked in nuclear division.		1976	767122
the effect of glucose and manganese on adenosine-3',5'-monophosphate levels during growth and differentiation of aspergillus nidulans.	the role of cyclic adenosine monophosphate (camp) during growth and development of aspergillus nidulans was investigated. in normal cultures the highest amount of camp, expressed on a dry weight basis, was found after 24 h of growth when still more than 5% glucose was present in the medium. after depletion of the medium even a slight fall in camp was noted. glucose concentrations ranging from 0.5-12% resulted in a slight decrease in the amount of camp as measured after 24 h of growth. cultures w ...	1976	179489
mutants of aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase.	ten mutants of aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase (nad-gdh) have been isolated, and their mutations (gdhb1 through gdhb10) have been shown to lie in the gdhb gene. in addition, a temperature-sensitive gdhb mutant (gdhb11) has been isolated. a revertant (designated r-5) of the mutant gdhb1 bears an additional lesion in the gdhb gene and has altered nad-gdh activity with altered km values for ammonia or ammonium ions and for alpha-ketogl ...	1976	173707
formation in the dark, of virus-induced deoxyribonuclease activity in anacystis nidulans, an obligate photoautotroph.	in anacystis nidulans, upon infection with cyanophage as-1, after a lag period of 1 h the level of deoxyribonuclease (dnase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. in contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, i.e. as late as 1 h prior to lysis. in complete darkness, the nuclease levels remained unaltered. however, when the infected cells were exposed to light for 1 or 2 h after infection ...	1976	177400
atmospheric mutagens. i. sulfur oxides and nitrogen oxides.		1976	134284
adenosine triphosphatase of aspergillus nidulans: effect of growth temperature.		1976	140148
effect of the area gene on regulation of arginine catabolism in aspergillus nidulans.	the area gene which is known to be involved in ammonium repression in aspergillus nidulans was found to participate in regulation of arginine catabolism. mutations in this gene are hypostatic to mutations in arca, sudpro and suepro genes which are responsible for regulation of synthesis of arginine catabolic enzymes.	1976	62492
basic and neutral amino acid transport in aspergillus nidulans.	arginine and methionine transport by aspergillus nidulans mycelium was investigated. a single uptake system is responsible for the transport of arginine, lysine and ornithine. transport is energy-dependent and specific for these basic amino acids. the km value for arginine is 1 x 10(-5) m, and vmax is 2-8 nmol/mg dry wt/min; km for lysine is 8 x 10(-6) m; kt for lysine as inhibitor of arginine uptake is 12 mum, and ki for ornithine is mm. on minimal medium, methionine is transported with a km of ...	1976	1466
analysis of acetate non-utilizing (acu) mutants in aspergillus nidulans.	genetic analysis of 119 acetate non-utilizing (acu) mutants in aspergillus nidulans revealed ten new loci affecting acetate metabolism in addition to the three previously recognized on the basis of resistance to fluoroacetate and acetate non-utilization. the enzyme lesions associated with mutations at seven of the acu loci are described. these are: faca (= acua), acetyl-coa synthase; acud, isocitrate lyase; acue, malate synthase; acuf, phosphoenolpyruvate carboxykinase; acug, fructose 1,6-diphos ...	1976	3622
assimilation of ammonia and growth of biotin deficient aspergillus nidulans.	biotin deficiency in aspergillus nidulans has been found to increase the uptake of ammonium ions, associated with a marked increase in the activity of nadp-linked glutamate dehydrogenase, which is found to be the major route of ammonia assimilation in this culture. the results obtained are discussed with respect to the growth of aspergillus nidulans during biotin deficiency.	1976	5283
chlorate toxicity in aspergillus nidulans. studies of mutants altered in nitrate assimilation.	it had previously been held that chlorate is not itself toxic, but is rendered toxic as a result of nitrate reductase-catalysed conversion to chlorite. this however cannot be the explanation of chlorate toxicity in aspergillus nidulans, even though nitrate reductase is known to have chlorate reductase activity. among other evidence against the classical theory for the mechanism of chlorate toxicity, is the finding that not all mutants lacking nitrate reductase are clorate resistant. both chlorat ...	1976	8697
differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains of aspergillus nidulans.	the antimitotic compound methyl benzimidazol-2-yl carbamate (mbc) formed a complex in vitro with a protein present in mycelial extracts of fungi. the binding protein of aspergillus nidulans showed a set of properties which is unique for tubulin. binding occurred rapidly at 4 degrees c and was competitively inhibited by oncodazole and colchicine. other inhibitors of microtubule function such as podophyllotoxin, vinblastine sulfate, melatonin, and griseofulvin did not interfere with binding of mbc ...	1977	12184
in vivo and in vitro studies of nitrate reductase regulation in asperillus nidulans.	induced wildtype cells of a. nidulans rapidly lost nadph--linked nitrate reductase activity when subjected to carbon and or nitrogen starvation. a constitutive mutant at the regulatory gene for nitrate reductase, nir ac 1, rapidly lost nitrate reductase activity upon carbon starvation. this loss of activity is thought to be due to a decrease in the nadph concentration in the cells. cell free extracts from wildtype cells grown in the presence of nitrate, rapidly lost their nitrate reductase activ ...	1977	17826
induction of the acetamidase of aspergillus nidulans by acetate metabolism.	growth tests and enzyme determinations strongly suggest that the acetamidase of aspergillus nidulans is induced by a product of acetate metabolism rather than the substrate, acetamide. the cis-dominant mutation, amdi9, which is closely linked to amds, the structural gene for the acetamidase, results in greatly increased sensitivity to induction by acetate metabolism. propionate, l-threonine, and ethanol also result in acetamidase induction. mutations in the faca, facb, and facc genes, which lead ...	1977	19418
mutagenic and recombinogenic action of pesticides in aspergillus nidulans.	thirteen pesticides, aminotriazole, benomyl, captafol, captan, dalapon-na, dichlorvos, dinobuton, dodine, ioxynil, mecoprop, neburon, picloram and tordon were tested for ability to induce (1) point mutations to 8-azaguanine resistance, (2) mitotic crossing-over, and (3) mitotic non-disjunction and haploidization in aspergillus nidulans. tests were performed at three different phs, i.e. 4.5, 7, 8.2. three of the pesticides, captan , captafol and dichlorvos induced point mutations; dichlorvos also ...	1977	22812
increased and decreased sensitivity to carbon catabolite repression of enzymes of acetate metabolism in mutants of aspergillus nidulans.	the crea204, creb15 and crec27 mutations have been shown to cause carbon catabolite derepression of acetly coa synthase and isocitrate lyase in aspergillus nidulans. a recessive mutation, cre-34, which is linked to the crec gene, results in these enzymes being more sensitive than cre or wildtype strains to catabolite repression. the acetamidase levels of strains containing cre mutations have been investigated and provide support for the hypothesis that an acetate metabolite, rather than acetamid ...	1977	23491
identification of an intramitochondrially synthesized proteolipid associated with the mitochondrial atpase complex as the product of a mitochondrial gene determining oligomycin resistance in aspergillus nidulans.		1977	142636
nuclear-extranuclear interactions affecting oligomycin resistance in aspergillus nidulans.	the extranuclear mitochondrial oligomycin-resistant mutation of aspergillus nidulans, (olia1), was transferred asexually into four nuclear oligomycin-resistant strains of different phenotypes. in all four cases, the possession of the nuclear plus extranuclear mutation led to an increase in the in vivo level of oligomycin resistance. in two cases, the altered cytochrome spectrum and impaired growth ability determined by (olia1) were suppressed by the nuclear mutations. in the third case, the in v ...	1977	144864
effect of growth temperature on the cell constituents of aspergillus nidulans.		1977	202564
genetic damage induced by ethyl alcohol in aspergillus nidulans.	heterozygous diploid conidia of aspergillus nidulans were treated during germination with ethyl alcohol in concentrations ranging from 0.25% to 20% (v/v). the diploid strain carried three recessive conidial color mutations, in addition to genetic markers on all eight pairs of linkage groups. it was thereby possible to detect events of crossing over, non-disjunction, and mutation. an increase in the dose of ethanol was associated with a decrease in conidial viability and an increase in the relati ...	1977	319353
pleiotropic mutants of aspergillus nidulans altered in carbon metabolism.	mutants altered in carbon catabolite regulation have been isolated by selecting for mutants of the area217 strain capable of using acetamide as the sole nitrogen source in the presence of sucrose. in addition to crea mutants described previously be arst and cove, strains with mutations in two new genes, creb and cre c, have been found. the creb and crec mutants grow poorly on some sole carbon sources and have low levels of some enzymes of carbon catabolism e.g. beta-galactosidase and d-quinate d ...	1977	320455
interspecific protoplast fusion and complementation in aspergilli.		1977	321242
two unlinked genes for the pyruvate dehydrogenase complex in aspergillus nidulans.	the activity of the overall pyruvate dehydrogenase complex was found to be similar in extracts of aspergillus nidulans after growth on either sucrose or acetate. eight mutants lacking the activity of this complex were found among some 200 glycolytic mutants selected for their inability to grow on sucrose. the absence of pyruvate dehydrogenase complex activity was also confirmed for a mutant, g6 (pdha1), isolated previously. studies with the mutants supported the existence of two unlinked genes, ...	1977	321417
effects of ethidium bromide in diploid and duplication strains of aspergillus nidulans.	unstable duplication and diploid strains of aspergillus nidulans were treated with ethidium bromide, and it was shown that this drug reduces the number of sectors produced by such strains. the mechanisms which could be responsible for the partial stabilization of the strains are discussed and it is suggested that a similar mechanism is responsible for the production of sectors in both strains. it is also suggested that ethidium bromide could be useful for the reduction of instability of industri ...	1977	323033
the effects of 1,4-diaminobutanone on polyamine synthesis in aspergillus nidulans.		1977	323046
some genetical aspects of ornithine metabolism in aspergillus nidulans.	a possible minor route of ornithine catabolism in aspergillus nidulans might begin with the ornithine decarboxylase reaction and end with the succinic semialdehyde dehydrogenase reaction. it is therefore of interest that the putative structural genes for these two enzymes, pua and ssua, respectively, are tightly linked group ii. however, this linkage is unlikely to have regulatory significance because ilea, the structural gene for threonine dehydratase, separates them. the gene order in this reg ...	1977	325361
resistance and mitotic instability to chloroneb and 1,4-oxathiin in aspergillus nidulans.	mutants resistant to two fungicides, chloroneb (1,4-dichloro-2,5-dimethoxybenzene) and vitavax (2,3-dihydro-5-carboxanilido-6-methyl-1,4-oxathiin) were spontaneously obtained from a strain of aspergillus nidulans with frequencies of 12.5 and 1.1 respectively, in 10(8) conidia. one chloroneb-resistant mutant (chl 1) segregated as a single gene and was mapped in linkage group iv. it also caused a partial dependence of the strain on the fungicide and was semi-dominant. the mutant resistant to vitav ...	1977	327307
meiotic and mitotic recombination in aspergillus and its chromosomal aberrations.		1977	327767
the genetic control of molybdoflavoproteins in aspergillus nidulans. a xanthine dehydrogenase i half-molecule in cnx- mutant strains of aspergillus nidulans.	the cnx- group of mutants of aspergillus nidulans lacks xanthine dehydrogenase (xanthine: nad+ oxidoreductase, ec 1.2.1.37) and nitrate reductase (ec 1.6.6.3) activities and are thought to be defective in the synthesis of a molybdenum-containing cofactor, 'cnx', common to xanthine dehydrogenase and nitrate reductase [pateman, j.a., rever, b.m., cove, d.j. and roberts, d.b. (1964) nature (lond.) 201, 58-60]. the cnx cofactor has a role in maintaining the aggregated multimeric structure of nitrate ...	1977	330163
regulation of glucose transport in aspergillus nidulans.	pyruvate and acetate inhibited the uptake of glucose by aspergillus nidulans; although there were significant variations in glucose uptake rate, the intracellular concentration of acetate was almost identical in biotin-supplemented, normal and deficient cells. the in vitro activity of glucose-binding protein was not affected by biotin, avidin, acetate or acetyl-coa.	1977	330193
l-asparaginase activity in the fungus aspergillus nidulans.		1977	330277
[genetic control of recombination processes in aspergillus nidulans. i. effect of uvs-mutations on the frequency of spontaneous and ultraviolet ray-induced intergenic recombination].	effect of 3 uvs mutations (uvs 12, 19 and 25) on recombination processes in aspergillus nidulans is studied. all the mutations are found either to affect the fertility of carp bodies and germination ability of askospores, or result in complete inability of heterokaryons to form cleistocarpia. two mutations change the frequency of spontaneous meitotic crossing-over at pro-paba region of the chromosome i and do not affect the rate of mitotic recombination at w-centromeric region of the chromosome ...	1977	330315
recent advances in basic mutation research.		1977	331099
the metabolism of phenylacetic acid by aspergillus fumigatus atcc 28282: identification of 2,6-dihydroxyphenylacetic acid.	aspergillus fumigatus atcc 28282 converted phenylacetic acid into a new dihydroxylated compound (2,6-dihydroxyphenylacetic acid) which was identified as 2,6-dimethoxyphenylacetic acid methyl ester. two other new metabolites have been isolated also and identified as orthohydroxyphenylacetic acid and meta-hydroxyphenylacetic acid.	1977	332293
[ultrastructural investigations on antimitotic activity of methylbenzimidazol-2-ylcarbamate (mbc) and its influence on replication of the nucleus-associated organelle ("centriolar plaque", "mtoc", "kce") in aspergillus nidulans (author's transl)].		1977	333514
aspergillus nidulans mutant lacking alpha-(1,3)-glucan, melanin, and cleistothecia.	a mutation in aspergillus nidulans led to a loss of both melanin and alpha-(1,3)-glucan, a major wall polysaccharide. in addition, the mutation prevented the formation of cleistothecia. mutant walls contained increased amounts of beta-(1,3)-glucan and galactose polymers, and electron micrographs indicated that they had lost the outermost wall layer. such walls were more readily digested by lytic enzymes, and this increased susceptibility to hydrolysis was due to the absence of alpha-(1,3)-glucan ...	1977	334748
the induction of mutations to 2-thioxanthine resistance in inhibitor depleted conidia of aspergillus nidulans by gamma-radiation in the presence of oxygen or nitrogen.	a strain of aspergillus nidulans has been used to study the inactivating and mutagenic effect of 60cogamma-rays in the presence of oxygen or nitrogen. mutation was studied by the 2-thioxanthine system which selectively detects forward mutation at a number of gene loci (at least 16). mutants resistant to conidial pigmentation effects of 2-thioxanthine can be divided into four main classes (2txr, hx, uay and cnx) and three of these classes (hx, uay and cnx) can be further characterized at the gene ...	1977	335238
the basis for an apparent auxotrophy for reduced sulphur metabolites in sf- mutants of aspergillus nidulans.		1977	336463
a mutation in aspergillus nidulans which affects the regulation of nitrite reductase and is tightly linked to its structural gene.		1977	337118
regulation of ammonia uptake in aspergillus nidulans.	the ammonia uptake in a. nidulans was found to be linear for about 20 min, and was proportional up to 1.5 mg/ml dry cell density. the transport of ammonia does not involve energy. normal and biotin deficient a. nidulans showed an identical km-values of 10.26 x 10(-5) m ammonia for uptake. the uptake of ammonium ion has been shown to be regulated by the intracellular concentration of ammonia.	1977	338337
the effect of 1,4-diaminobutanone on the stability of ornithine decarboxylase from aspergillus nidulans.	1,4-diaminobutanone, a competitive inhibitor of ornithine decarboxylase in aspergillus nidulans, is able to increase the half-life of this enzyme and thus stimulate an increase in its activity in vivo. it also protects ornithine decarboxylase against proteolysis by chymotrypsin in vitro.	1977	339910
[genetic control of the sensitivity of aspergillus nidulans to mutagenic factors. vii. inheritance of cross-sensitivity to different mutagenic factors by uvs-mutants].	to study the inheritance of the sensitivity to uv, x-rays, methylmethanesulphonate (mms), nitrosoguanidine (ng) and nitrous acid (na) in five uvs mutants of aspergillus nidulans, having multiple sensitivity to these factors, the sensitivity of recombinants obtained from crossing uvs mutants with uvs+ strain, resistant to all the factors analysed, and uvs leads to uvs+ revertants is investigated. four uvs mutants (15, 17, 19 and 26) are found to have a nomogenic control of sensitivity to differen ...	1977	352800
genetic analysis of mutations of low (rec) and very high (pop) mitotic-recombination frequency in aspergillus nidulans.		1977	340885
extranuclear recombination in aspergillus nidulans: closely-linked multiple chloramphenicol- and oligomycin-resistance loci.	a nuclear, chloramphenicol-sensitive mutant cas-1 has been isolated which is cross sensitive to a number of drugs, including oligomycin and cycloheximide. approximately one-third of the chloramphenicol-resistant mutants isolated from mutagenized conidia of this strain were found to be extranuclear, and exhibited a variety of phenotypes. one of these mutants, designated (camb51), was slow growing on drug-free medium and recombined at low frequency with the previously described mutant (cama112) (g ...	1977	340908
growth, glucose metabolism and melanin formation in biotin-deficient aspergillus nidulans.	biotin deficiency resulted in an increased growth rate of aspergillus nidulans. the activities of hexokinase and aldolase were not much changed during the growth cycle, but activities of glucose-6-phosphate dehydrogenase and nadp-linked glutamate dehydrogenase increased significantly during the exponential phase. this change was remarkable during biotin deficiency. in contrast to the higher growth rate and respiration rate during biotin deficiency the activities of nad(p)h oxidoreductases were l ...	1977	402307
glucan common to the microcyst walls of cyst-forming bacteria.	chemical analysis indicated that d-glucose is tha major neutral monosaccharide present in the microcysts of a range of gram-negative bacteria. varying amounts of other neutral sugars were found. the glucose was mainly present as a glucan that could be extracted from microcysts of representative strains with alkali or mild acid treatment. the glucan could be identified as an alpha-1,3-linked polymer on the basis of (i) periodate resistance of the extracted polymer and the material present in micr ...	1977	402353
temperature dependence on the delayed fluorescence of chlorophyll a in blue-green algae.	1. the delayed fluorescence of chlorophyll a was measured with a phosphoroscope by changing the temperature in a range of room temperatures in intact cells of blue-green algae, anacystis nidulans, two strains of anabaena variabilis and plectonema boryanum, and other kinds of algae, cyanidium caldarium and chlorella pyrenoidosa. the induction of delayed fluorescence remarkably depended on the temperature of measurment. nevertheless, the induction pattern was characterized by three levels of inten ...	1977	403943
interference of cellular ferric ions with dna extraction and the application to methods of dna determination.		1977	405886
photosynthetic activity of diimidoester-modified cells, permeaplasts, and cell-free membrane fragments of the blue-green alga anacystis nidulans.	on treating the blue green alga anacystis nidulans with dimethylsuberimidate up to 70% of the free nh2 of the photosynthetic membrane is amidinated, and presumably inter- and intramolecular cross-links are established in the membrane proteins. amidination destroys the ability of a. nidulans to photoreduce hco3(-) but leaves the photochemical activities of photosystems ii and i nearly intact. with added electron acceptors, photosynthetic o2 evolution can be demonstrated both with permeable cells ...	1977	409432
occurrence of a major protein associated with fruiting body development in neurospora and related ascomycetes.	electrophoretic and immunological analysis of fruiting body (perithecial) extracts demonstrates the occurrence of a major phase-specific perithecial protein in all neurospora species and in the closely related gelasinospora cerealis and sordariafimicola. the perithecial proteins from these different species fall into a number of groups with different electrophoretic mobilities. they appear to be immunologically closely related but not identical to one another even within the same genus, with onl ...	1977	410027
adsorption of cyanophage as-1 to unicellular cyanobacteria and isolation of receptor material from anacystis nidulans.	cells of unicellular cyanobacteria of typological group ia, containing approximately 50 mol% guanine + cytosine (g+c) in their dna (r. y. stanier, r. kunisawa, m. mandel, and g. cohen-bazire, bacteriol. rev. 35:171-205, 1971), were susceptible to infection by the cyanophage as-1. cyanobacteria of the same typological group, containing approximately 65 mol% g+c in their dna, did not adsorb the cyanophage as-1 or adsorbed it at a low rate. as-1 was not propagated by any of the investigated strains ...	1978	413935
culture and antigen variants of aspergillus.		1978	416063
meiotic recombination in a duplication strain of aspergillus nidulans.		1978	344133
the effects of coumarin on the frequency of deletions in a duplication strain of aspergillus nidulans.	strains of a. nidulans with a chromosome segment in duplicate show instability resulting from deletions in either of the duplicate segments. in dp (i, ii) strains, with the terminal segment of ir attached terminally to iir, spontaneous deletions occur most frequently, though not exclusively, from the translocated segment. coumarin, at concentrations which did not affect viability viability or growth rate, enhanced the instability of dp (i, ii) strains by selectively increasing only the deletion ...	1978	345093
supersuppressors in aspergillus nidulans.	simultaneous reversion of mutations in two different aspergillus nidulans loci ada and metg was found to be due monogenic suppressor mutations. preliminary evidence for the existance of supersuppressors in a. nidulans is presented.	1978	345096
restriction endonuclease cleavage map of mitochondrial dna from aspergillus nidulans.	mitochondrial dna of the ascomycete fungus aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases eco r i, hind ii, hind iii and bgl ii into 3, 7, 9 and 5 fragments, respectively. the relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole dna and by complete and partial redigestion of isolated restriction fragments.	1978	345242
mutations in nira gene of aspergillus nidulans and nitrogen metabolism.		1978	347305
sporulation competence in aspergillus nidulans: a role for iron in development.	there is a difference in the response of dna from mycelial extracts of aspergillus nidulans to hot acid hydrolysis depending upon the state of sporulation competence. the dna in incompetent culture mycelia is not hydrolyzable while the dna in competent culture is hydrolyzable. the inhibition of dna hydrolysis is due to the presence of iron. although the concentration of iron decreases in mycelia during growth, there is sufficient iron present in competent mycelia to inhibit dna hydrolysis. the c ...	1978	350417
the nature of an initiator constitutive mutation in aspergillus nidulans.		1978	351427
characterization of a new choline locus in aspergillus nidulans and its significance for choline metabolism.		1978	376401