Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| molecular analysis of an nad-dependent alcohol dehydrogenase from the zygomycete mucor circinelloides. | nad-dependent alcohol dehydrogenase (adh) activity was detected mainly in the cytosol of aerobically cultured mycelium and in anaerobically grown yeast cells of mucor circinelloides. adh levels were about 2.5-fold higher in yeast cells than in mycelium; zymogram analysis suggested that the same adh enzyme is produced in both developmental stages. the enzyme, named adh1, was purified to homogeneity from yeast cells, using ion-exchange and affinity chromatography. the active adh1 appears to be a h ... | 2005 | 16179992 |
| production of lactic acid and fungal biomass by rhizopus fungi from food processing waste streams. | this study proposed a novel waste utilization bioprocess for production of lactic acid and fungal biomass from waste streams by fungal species of rhizopus arrhizus 36017 and r. oryzae 2062. the lactic acid and fungal biomass were produced in a single-stage simultaneous saccharification and fermentation process using potato, corn, wheat and pineapple waste streams as production media. r. arrhizus 36017 gave a high lactic acid yield up to 0.94-0.97 g/g of starch or sugars associated with 4-5 g/l o ... | 2005 | 16208461 |
| influence of autoclaved fungal materials on spearmint (mentha spicata l.) growth, morphogenesis, and secondary metabolism. | the influence of autoclaved fungal materials such as culture filtrate, freeze-dried mycelium (fdm), mycelium suspension, and spore suspension (ss) on the growth, morphogenesis, and carvone production of spearmint (mentha spicata l.) plants was studied. fungal materials were either applied as a drench or spray on the plants. spearmint plants (cv. "294099") drenched with ss (1 x 10(8) spores/ml) of trichoderma reesei showed no significant differences in leaf numbers, root numbers, or shoot numbers ... | 2005 | 16222794 |
| purification and characterization of a new endo-1,4-beta-d-glucanase from beltraniella portoricensis. | a new endoglucanase, designated bce1, produced by beltraniella portoricensis, was purified from the culture supernatant. the n-terminal amino acid sequence suggests that bce1 belongs to family 45 glycoside hydrolase (family 45 endoglucanase). the molecular mass of bce1 was found to be 40 kda by sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page). the optimum ph for the carboxymethyl cellulase (cmcase) activity of bce1 was 4.5, and the optimum temperature was 55 degrees c. among f ... | 2005 | 15973054 |
| genetic identification and detection of human pathogenic rhizopus species, a major mucormycosis agent, by multiplex pcr based on internal transcribed spacer region of rrna gene. | mucormycosis is an invasive opportunistic infection caused by fungi belonging to the order mucorales. due to the lack of laboratory tests, the diagnosis of mucormycosis is notoriously difficult. added with its rapid progression as well as the debilitated state of the patients who contract the disease, mortality is extremely high. | 2005 | 15978416 |
| lead biosorption study with rhizopus arrhizus using a metal-based titration technique. | acid-base and metal-based potentiometric titration methods were used to analyze sorption mechanisms of lead by rhizopus arrhizus fungal biomass. biosorption was not considered globally but as the result of successive sorption reactions on various binding sites with different selectivities. precipitation occurred rapidly when lead concentration increased. lead was sorbed essentially by carboxylic groups and by phosphates and sulfonates (less abundant) of the organic matter. the lead affinity to c ... | 2005 | 15979634 |
| in vitro susceptibility of 15 strains of zygomycetes to nine antifungal agents as determined by the nccls m38-a microdilution method. | the in vitro susceptibility of 15 strains of six genera of zygomycetes including rhizopus oryzae (rhizopus arrhizus), rhizopus stolonifer, mucor circinelloides (three), absidia corymbifera (three), rhizomucor pusillus (three), cunninghamella bertholletiae (two), and syncephalastrum racemosum (two) to nine antifungal agents were determined by the nccls m38-a broth microdilution method. geometric means of the minimal inhibitory concentrations (mic) were: amphotericin b 0.07 mg l(-1) (range 0.03-0. ... | 2005 | 15982206 |
| enhanced reactivity of rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: potential as a whole-cell biocatalyst in organic solvents. | immobilization of enzymes on some solid supports has been used to stabilize enzymes in organic solvents. in this study, we evaluated applications of genetically immobilized rhizopus oryzae lipase displayed on the cell surface of saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with alpha-agglutinin. compared to the activity of a commercial preparation of this lipase, the activity of the new preparation was 4.4 x 10(4)-fo ... | 2005 | 16085821 |
| pcr based identification and discrimination of agents of mucormycosis and aspergillosis in paraffin wax embedded tissue. | invasive fungal infections are often diagnosed by histopathology without identification of the causative fungi, which show significantly different antifungal susceptibilities. | 2005 | 16254108 |
| cerebral zygomycosis. | fifty-six patients with cerebral zygomycosis (mucormycosis) were seen during the period 1971-2001 in two tertiary care hospitals located in south india with tropical climate and catering to neurological diseases. forty-four patients had rhinocerebral and twelve patients had isolated central nervous system (cns) zygomycosis. of these, ten were culture proven (rhizopus oryzae in eight and mucor in two); 30 were diagnosed as probable and 16 were diagnosed possible; mixed infections were seen in thr ... | 2005 | 16262876 |
| [mucormycosis caused by rhizopus oryzae in a patient with atypical diabetes]. | 2005 | 16324575 | |
| post harvest spoilage of sweetpotato in tropics and control measures. | sweetpotato storage roots are subjected to several forms of post harvest spoilage in the tropical climate during transportation from farmers' field to market and in storage. these are due to mechanical injury, weight loss, sprouting, and pests and diseases. sweetpotato weevil is the single most important storage pest in tropical regions for which no control measures or resistant variety are yet available. several microorganisms (mostly fungi) have been found to induce spoilage in stored sweetpot ... | 2005 | 16371331 |
| [cutaneous mucormycosis and diabetes: about one observation]. | mucormycosis is a rare opportunistic infection but a fulminant disease. varying clinical forms have been described, including cutaneous localisations which are mainly observed in diabetic and burned patients. the cutaneous lesions induced by the mucormycosis affection are often atypical and gangrenous. we report a case of cutaneous mucormycosis in a tunisian patient with diabetic ketoacidosis. he developed cutaneous necrotic lesions at the low limb. the diagnosis of mucormycosis was not initiall ... | 2005 | 16425710 |
| [mycoses of the head and neck]. | the ever growing number of immunocompromized patients and increasing travel to areas where fungal diseases are endemic explain why in recent years mycoses have emerged as important infections in clinical practice. it is essential that pathologists and clinicians be knowledgeable about them. | 2005 | 16439931 |
| heterologous production of functional forms of rhizopus oryzae lipase in escherichia coli. | to date, expression of the lipase from rhizopus oryzae (rol) in escherichia coli always led to the formation of inclusion bodies and inactive protein. however, the production of active rol and its precursor prorol in soluble form was achieved when e. coli origami(de3) and pet-11d were used as expression systems. | 2005 | 16332903 |
| [heteroexpression of rhizopus arrhizus delta6-fatty acid desaturase gene in pichia pastoris]. | delta6-fatty acid desaturase is a membrane-bound enzyme, which is rate-limiting for the biosynthesis of polyunsaturated fatty acids. a cdna sequence putatively encoding a delta6-fatty acid desaturase was isolated from rhizopus arrhizus nk300037 using rt-pcr and race methods in our previous work. sequence and function analysis indicated that this sequence was a novel delta6-fatty acid desaturase gene which had an open reading frame of 1377bp coding 458 amino acids of 52kd. the methylotrophic yeas ... | 2005 | 16468339 |
| cytokine responses and regulation of interferon-gamma release by human mononuclear cells to aspergillus fumigatus and other filamentous fungi. | there is substantial evidence that the production of proinflammatory cytokines is important in host resistance to invasive aspergillosis. knowledge of the host response towards other filamentous fungi is scarce, as most studies have focused on aspergillus fumigatus. in addition, interferon-gamma (ifngamma) plays a crucial role in the control of invasive aspergillosis, but little is known about the regulation of ifngamma after stimulation of mononuclear cells by a. fumigatus. cytokine responses t ... | 2005 | 16396246 |
| functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan. | a survey of the three kinetoplastid genome projects revealed the presence of three putative front-end desaturase genes in leishmania major, one in trypanosoma brucei and two highly identical ones (98%) in t. cruzi. the encoded gene products were tentatively annotated as delta8, delta5 and delta6 desaturases for l. major, and delta6 desaturase for both trypanosomes. after phylogenetic and structural analysis of the deduced proteins, we predicted that the putative delta6 desaturases could have del ... | 2006 | 16403015 |
| divergent evolutionary lines of fungal cytochrome c peroxidases belonging to the superfamily of bacterial, fungal and plant heme peroxidases. | novel open reading frames coding for cytochrome c peroxidase (ccp) belonging to the superfamily of bacterial, fungal, and plant heme peroxidases were analyzed in the available fungal genomes. multiple sequence alignment of 71 selected peroxidase genes revealed the presence of three conserved regions essential for their function: one on the distal and two on the proximal side of the prosthetic heme group. conserved sequence motifs on the proximal heme side are peculiar for ccps and are responsibl ... | 2006 | 17126331 |
| cloning, disruption and protein secretory phenotype of the gas1 homologue of pichia pastoris. | the aim of the study was the identification, cloning and disruption of the gas1 homologue of pichia pastoris. gas1p is a glycoprotein anchored to the outer layer of the plasma membrane through a glycosylphosphatidylinositol (gpi) anchor. gas1p is a beta-1,3-glucanosyltransglycosylase (ec 2.4.1.-). this cross-linking enzyme highly affects the structure and permeability of the yeast cell wall. the gene coding for the gas1 homologue of p. pastoris was cloned by pcr, and its functionality was proven ... | 2006 | 17020547 |
| outbreak of cutaneous rhizopus arrhizus infection associated with karaya ostomy bags. | we investigated an outbreak involving 2 patients hospitalized at hospital a with cutaneous rhizopus arrhizus (oryzae) infections of surgically created stomas. | 2006 | 17029127 |
| amino acid regions of family 45 endoglucanases involved in cotton defibrillation and in resistance to anionic surfactants and oxidizing agents. | in the detergent industry, fungal endoglucanases are used to release microfibrils from the surfaces of dyed cellulosic fabrics to enhance color brightness. family 45 endoglucanase (glycoside hydrolase family 45, gh45) egl3 from humicola grisea is more resistant to anionic surfactants and oxidizing agents than family 45 endoglucanase rce1 from rhizopus oryzae, while in the present study, a catalytic domain of rce1 had higher defibrillation activity on dyed cotton fabrics than did that of egl3. to ... | 2006 | 17031034 |
| [screening of a low alcohol dehydrogenase activity mutant of rhizopus oryzae and the regulation of zn2+ and mg2+]. | ethanol is the main by-product in the fermentation broth of rhizopus oryzae as3.3461 for the production of high-optical purity l-lactic acid. alcohol dehydrogenase (adh) is the branch pathway enzyme that catalyzes the transformation of ethanol from pyruvate in rhizopus oryzae, which decreases the conversion rate of glucose to l-lactic acid. thus, screening the mutants with lower adh activity may increase lactate production dramatically. in present study, rhizopus oryzae as3.3461 was mutated with ... | 2006 | 17037060 |
| an early evolutionary origin for the minor spliceosome. | the minor spliceosome is a ribonucleoprotein complex that catalyses the removal of an atypical class of spliceosomal introns (u12-type) from eukaryotic messenger rnas. it was first identified and characterized in animals, where it was found to contain several unique rna constituents that share structural similarity with and seem to be functionally analogous to the small nuclear rnas (snrnas) contained in the major spliceosome. subsequently, minor spliceosomal components and u12-type introns have ... | 2006 | 17051219 |
| lactate and ethanol productions by rhizopus oryzae atcc 9363 and activities of related pyruvate branch point enzymes. | in rhizopus oryzae cultivations, lactate production decreased and ethanol and biomass productions increased as inoculated spore concentration increased. spore concentrations of 1 x 10(3) and 1 x 10(4) spores ml(-1) induced tightly packed clumps, whereas 1 x 10(5) and 1 x 10(6) spores ml(-1) induced filamentous growth. | 2006 | 17189176 |
| state variables monitoring by in situ multi-wavelength fluorescence spectroscopy in heterologous protein production by pichia pastoris. | state variables throughout non-induced and induced cultivations of pichia pastoris for the heterologous rhizopus oryzae lipase (rol) production were monitored with a multi-wavelength on-line fluorescence sensor. based on this work, the use of in situ multi-wavelength fluorometry combined with chemometrics models (pls-1 models) provided a quantitative prediction of biomass and substrates (glycerol and methanol) during non-induced and induced rol production. the mean prediction errors for both var ... | 2006 | 16488501 |
| the family 21 carbohydrate-binding module of glucoamylase from rhizopus oryzae consists of two sites playing distinct roles in ligand binding. | the starch-hydrolysing enzyme ga (glucoamylase) from rhizopus oryzae is a commonly used glycoside hydrolase in industry. it consists of a c-terminal catalytic domain and an n-terminal starch-binding domain, which belong to the cbm21 (carbohydrate-binding module, family 21). in the present study, a molecular model of cbm21 from r. oryzae ga (rogacbm21) was constructed according to pssc (progressive secondary structure correlation), modified structure-based sequence alignment, and site-directed mu ... | 2006 | 16509822 |
| rhizopus oryzae fungus cells producing l(+)-lactic acid: kinetic and metabolic parameters of free and pva-cryogel-entrapped mycelium. | spores of the filamentous fungus rhizopus oryzae were entrapped in macroporous poly(vinyl alcohol) cryogel (pva-cryogel). to prepare immobilised biocatalyst capable of producing l(+)-lactic acid (la), the fungus cells were cultivated inside the carrier beads. the growth parameters and metabolic activity of the suspended (free) and immobilised cells producing la in a batch process were comparatively investigated. the immobilised cells possessed increased resistance to high concentrations of accum ... | 2006 | 16523285 |
| lactic acid production from xylose by the fungus rhizopus oryzae. | lignocellulosic biomass is considered nowadays to be an economically attractive carbohydrate feedstock for large-scale fermentation of bulk chemicals such as lactic acid. the filamentous fungus rhizopus oryzae is able to grow in mineral medium with glucose as sole carbon source and to produce optically pure l(+)-lactic acid. less is known about the conversion by r. oryzae of pentose sugars such as xylose, which is abundantly present in lignocellulosic hydrolysates. this paper describes the conve ... | 2006 | 16528511 |
| lipase-catalyzed esterification of 2-monoricinolein for 1,2 (2,3)-diricinolein synthesis. | the purpose of this investigation was to develop conditions for producing 2-monoricinoleoyl dag. we used lipase-catalyzed hydrolysis of triricinolein to obtain 2-monoricinolein and thereafter synthesized 1,2(2,3)-diricinolein through esterification of 2-monoricinolein, using ricinoleic acid as the acyl donor. five different 1,3-specific immobilized lipases were tested for the initial methanolysis reaction: candida antarctica type b, rhizomucor miehei, rhizopus oryzae (rol), thermomyces lanuginos ... | 2006 | 16555475 |
| asymmetric reduction of 3-aryl-3-keto esters using rhizopus species. | ethyl 3-aryl-3-oxopropanoates (aryl: phenyl, 2-fluorophenyl, 3-nitrophenyl, and 4-nitrophenyl) were reduced enantioselectively to the corresponding (s)-alcohols by the fungus rhizopus arrhizus and other rhizopus sp. the best results were generally obtained with rhizopus arrhizus (wild type) and rhizopus nivius ncim 958 with 6h incubation. a longer incubation period led to ester hydrolysis followed by decarboxylation and microbial reduction for all the substrates especially the 3-nitrophenyl este ... | 2006 | 16616500 |
| specific characteristics of family 45 endoglucanases from mucorales in the use of textiles and laundry. | we examined the characteristics of family 45 endoglucanases (glycoside hydrolases family 45; gh45) from mucorales belonging to zygomycota in the use of textiles and laundry. the defibrillation activities on lyocell fabric of family 45 endoglucanases from mucorales, such as rce1 and rce2 from rhizopus oryzae, mce1 and mce2 from mucor circinelloides, and pce1 from phycomyces nitens, were much higher than those of the other family 45 endoglucanases. by contrast, family 45 endoglucanases from mucora ... | 2006 | 16636473 |
| biodegradable network elastomeric polyesters from multifunctional aliphatic carboxylic acids and poly(epsilon-caprolactone) diols. | biodegradable elastomeric network polyesters were prepared from multifunctional aliphatic carboxylic acids such as tricarballylic acid (yt) or meso-1,2,3,4-butanetetracarboxylic acid (xb) and poly(epsilon-caprolactone) (pcl) diols with molecular weights of 530, 1,250 and 2,000 g.mol-1. prepolymers prepared by a melt polycondensation were cast from dmf solution and postpolymerized at 280 degrees c for various periods of times to form a network. the resultant films were transparent, flexible and i ... | 2006 | 16676379 |
| bioconversion of d-psicose to d-tagatose and d-talitol by mucoraceae fungi. | rhizopus oryzae mya-2483, which cannot utilize d-psicose as a sole source of carbon, converted d-psicose to two other compounds. these compounds were identified by nmr and ir as d-tagatose and d-talitol. in this study, we describe for the first time the bioconversion of d-psicose to d-tagatose. various strains of mucoraceae fungi, to which r. oryzae mya-2483 belongs, exhibited conversion activity similar to that of r. oryzae mya-2483. there is the possibility that a considerable number of fungi ... | 2006 | 16716921 |
| lipase localization in rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production. | to identify the lipase responsible for the methanolysis activity of fungus whole-cell biocatalysts, the lipase localization of rhizopus oryzae cells was determined. western blot analysis showed that r. oryzae cells produce two types of lipase with different molecular masses of 34 and 31 kda; the former (rol34) was bound to the cell wall, whereas the latter (rol31) was mainly bound to the cell membrane. it was found that cell immobilization within reticulated polyurethane foam biomass support par ... | 2006 | 16716941 |
| a simple model-based control for pichia pastoris allows a more efficient heterologous protein production bioprocess. | a predictive control algorithm coupled with a pi feedback controller has been satisfactorily implemented in the heterologous rhizopus oryzae lipase production by pichia pastoris methanol utilization slow (mut(s)) phenotype. this control algorithm has allowed the study of the effect of methanol concentration, ranging from 0.5 to 1.75 g/l, on heterologous protein production. the maximal lipolytic activity (490 ua/ml), specific yield (11,236 ua/g(biomass)), productivity (4,901 ua/l . h), and specif ... | 2006 | 16732597 |
| the rising trend of invasive zygomycosis in patients with uncontrolled diabetes mellitus. | zygomycosis is an emerging infection worldwide. a study was conducted to understand its spectrum in the indian scenario. all patients diagnosed for invasive zygomycosis at a tertiary care center in north india from 2000-2004, were retrospectively analyzed. a total of 178 cases (mean average of 35.6 cases/year) of zygomycosis were diagnosed. rhino-orbito-cerebral type (54.5%) was the commonest presentation followed by cutaneous (14.6%), disseminated (9.0%), and gastrointestinal (8.4%) zygomycosis ... | 2006 | 16772227 |
| a case of rhinocerebral zygomycosis caused by rhizopus arrhizus. | 2006 | 16796654 | |
| plasmids for expression of heterologous proteins in rhizopus oryzae. | rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. in this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in r. oryzae. three plasmid vectors have been created using either the glucoamylase a (amya), pyruvate decarboxylase (pdca), or phosphoglycerate kinase (pgk1) promoters to drive expression of heterologous proteins. a ... | 2006 | 16804680 |
| empyema thoracis due to rhizopus oryzae in an allogenic bone marrow transplant recipient. | we describe a case of empyema thoracis caused by rhizopus oryzae diagnosed in an allogenic bone marrow transplant patient with acute lymphocytic leukemia. the isolate of r. oryzae was recovered from three pleural effusion specimens, which were black in color. it was identified on the basis of characteristic colonial appearance and microscopic findings, as well as the partial sequencing of rrna genes. the patient died of uncontrolled r. oryzae empyema thoracis and concomitant nosocomial infection ... | 2006 | 16805096 |
| biochemical and molecular characterization of a lipase produced by rhizopus oryzae. | a novel strain of rhizopus oryzae wpg secretes a noninduced lipase (rolw) in the culture medium; purified rolw is a protein of 29 kda, the 45 n-terminal amino acid residues were sequenced, this sequence is very homologous to rhizopus delemar lipase (rdl), rhizopus niveus lipase (rnl) and r. oryzae lipase (rol29) sequences; the cloning and sequencing of the part of the gene encoding the mature rolw, shows two nucleotides differences with rdl, rnl and rol29 sequences corresponding to the change of ... | 2006 | 16842350 |
| relationship between metabolism and biomass of medically important zygomycetes. | little is known about the relationships between metabolic activity and fungal biomass or time of incubation for medically important fungal pathogens. understanding these relationships may be especially relevant for rapidly growing organisms, such as zygomycetes. a range of inocula of five clinical isolates of zygomycetes (one each of rhizopus oryzae,rhizopus microsporus, cunninghamella bertholletiae, mucor circinelloides and absidia corymbifera) were incubated for 6, 8, 12, 24 and 48 h, after wh ... | 2006 | 16882609 |
| primary renal zygomycosis due to rhizopus oryzae. | primary renal zygomycosis is a rare fungal infection, and only 45 cases have been reported in the literature. the major etiologic agents of the disease were mucor spp., rhizomucor spp., rhizopus spp. and absidia spp. here we report a case of primary renal zygomycosis due to rhizopus oryzae infection. the patient had systemic lupus erythematosus and was treated with corticosteroids. he had frequent micturition, urodynia and passed amorphous and membranous-looking masses through the urethra severa ... | 2006 | 16882613 |
| construction of a pichia pastoris cell-surface display system using flo1p anchor system. | a pichia pastoris cell-surface display system was constructed using a flo1p anchor system, which was developed in saccharomyces cerevisiae. the lipase from rhizopus oryzae with a pro sequence (prorol) was used as the model protein and was genetically fused to the anchor consisting of amino acids 1-1099 of flo1p (fs anchor). the resulting fusion protein fsprorol was expressed under the control of the alcohol oxidase 1 promoter (paox1). the fluorescence microscopy of immunolabeled p. pastoris cell ... | 2006 | 16889374 |
| enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of (r,s)-1-benzyloxy-3-chloro-2-propyl monosuccinate. | rhizopus oryzae lipase (rol) was displayed on the cell surface of saccharomyces cerevisiae via the flo1 n-terminal region (1100 amino acids), which corresponds to a flocculation functional domain. the activity of lipase-displaying yeast whole-cell biocatalysts was enhanced 7.3-fold by incubation of the yeast cells at 20 degrees c in distilled water for 8 days after 8 day cultivation. the amount of lipase molecules present in cell wall and intracellular fractions was found to be increased 4.5- an ... | 2006 | 16889376 |
| adsorption behavior of rhodamine b on rhizopus oryzae biomass. | the removal of a carcinogenic dye rhodamine b (c. i. 45170) from wastewater by biomass of different moulds and yeasts is described. among all of the fungal species tested, the biomass of rhizopus oryzae mtcc 262 is found to be the most effective. dye adsorption reaches maximum with the biomass harvested from the early stationary phase of growth. the optimum temperature and ph for adsorption are observed to be 40 degrees c and 7.0, respectively. the adsorption rate is very fast initially and atta ... | 2006 | 16893225 |
| optimization of l-(+)-lactic acid production using pelletized filamentous rhizopus oryzae nrrl 395. | lactic acid is used as a food additive for flavor and preservation and a precursor in the development of poly-lactic acid, a product used to make biodegradable plastics and textiles. rhizopus oryzae nrrl 395 is known to be a strain that produces optically pure l-(+)-lactic acid. the morphology of rhizopus cultures is complex, forming filamentous, clumps, and pellet mycelia. different morphology growth has significant effects on lactic acid production. in bioreactors, the filamentous or clump myc ... | 2006 | 16915692 |
| deferiprone iron chelation as a novel therapy for experimental mucormycosis. | patients treated with the iron chelator deferoxamine are known to be more susceptible to mucormycosis. however, while deferoxamine is an iron chelator from the perspective of the human host, deferoxamine actually serves as a siderophore, delivering free iron to rhizopus oryzae, the major cause of mucormycosis. other iron chelators, including deferiprone, which do not deliver iron to r. oryzae have been described. we therefore sought to determine whether iron-chelation therapy with deferiprone wo ... | 2006 | 16928702 |
| functionality of selected strains of moulds and yeasts from vietnamese rice wine starters. | the role of starch-degrading mycelial fungi, and the alcohol production and ethanol tolerance of the yeasts isolated from selected vietnamese traditional rice wine starters were examined, and optimum conditions for these essential steps in rice wine fermentation were determined. of pure isolates from vietnamese rice wine starters, mould strains identified as amylomyces rouxii, amylomyces aff. rouxii, rhizopus oligosporus and rhizopus oryzae, were superior in starch degradation, glucose productio ... | 2006 | 16943022 |
| exploring amino acids responsible for the temperature profile of glycoside hydrolase family 45 endoglucanase egl3 from humicola grisea. | egl3 and rce1 are glycoside hydrolase family 45 endoglucanases isolated from humicola grisea and rhizopus oryzae respectively. the amino acid sequences of the two endoglucanases are homologous; on the other hand, the optimum temperature of egl3 is higher than that of rce1. in this study, four chimeric endoglucanases, named er1, er2, er3 and er4, in which one of four sequential amino acid regions of the egl3 catalytic domain (cad) was replaced by the corresponding rce1 amino acids, were construct ... | 2006 | 16960377 |
| a rhizopus oryzae strain isolated from resected bone and soft tissue specimens from a sinonasal and palatal mucormycosis case. report of a case and in vitro experiments of yeastlike cell development. | we report a histologically and mycologically proven sinonasal mucormycosis case causing palatal necrosis in a nondiabetic patient with renal failure. mycological examination of giemsa stained imprinted tissue preparations revealed abundant yeast-like cells besides the typical mucoraceous hyphae. the fungus was isolated from surgical specimens and identified as rhizopus oryzae by phenotypic and genotypic tests. laboratory studies were performed to investigate the association of the yeast-like cel ... | 2006 | 16966168 |
| molecular basis for enhanced activity of posaconazole against absidia corymbifera and rhizopus oryzae. | posaconazole and itraconazole were more potent inhibitors of ergosterol synthesis, in both intact cells and cell extracts from absidia corymbifera and rhizopus oryzae, than voriconazole and fluconazole. similarly, expression of cyp51 from r. oryzae in saccharomyces cerevisiae significantly increased resistance to fluconazole and voriconazole but not to posaconazole and itraconazole. | 2006 | 16966400 |
| optimization of l-(+)-lactic acid production using pelletized filamentous rhizopus oryzae nrrl 395. | lactic acid is used as a food additive for flavor and preservation and a precursor in the development of poly-lactic acid, a product used to make biodegradable plastics and textiles. rhizopus oryzae nrrl 395 is known to be a strain that produces optically pure l-(+)-lactic acid. the morphology of rhizopus cultures is complex, forming filamentous, clumps, and pellet mycelia. different morphology growth has significant effects on lactic acid production. in bioreactors, the filamentous or clump myc ... | 2006 | 18563658 |
| zygomycosis of the penis due to rhizopus oryzae successfully treated with surgical debridement and a combination of high-dose liposomal and topical amphotericin b. | 2006 | 17179006 | |
| molecular identification of zygomycetes from culture and experimentally infected tissues. | mucormycosis is an emerging infection associated with a high mortality rate. identification of the causative agents remains difficult and time-consuming by standard mycological procedures. in this study, internal transcribed spacer (its) sequencing was validated as a reliable technique for identification of zygomycetes to the species level. furthermore, species identification directly from infected tissues was evaluated in experimentally infected mice. fifty-four zygomycetes strains belonging to ... | 2006 | 16455881 |
| rapid susceptibility testing of medically important zygomycetes by xtt assay. | the xtt colorimetric assay quantifies fungal growth by measuring fungal metabolism and has been used successfully for susceptibility testing of aspergillus species after 24 and 48 h of incubation. in the present study using 14 clinical isolates of zygomycetes (rhizopus oryzae [5 isolates], cunninghamella spp. [3 isolates], mucor spp. [3 isolates], and absidia corymbifera [3 isolates]), significant metabolic activity was demonstrated before visual or spectrophotometric detection of fungal growth ... | 2006 | 16455912 |
| association constants of pb2+ with binding sites of fungal biomass using metal-based titrations. | biosorption is perceived as an alternative method for toxic heavy metal removal/recovery from aqueous effluents. this work focused on derivation of some key quantitative physico-chemical characteristics of a representative biosorbent material required for its further effective exploitation. the newly developed acid-base and metal-based titrator allowed the characterization of the chemisorption active sites of rhizopus arrhizus biomass and the study of their metal affinity. this experimental appr ... | 2006 | 16457181 |
| secretion of pro- and mature rhizopus arrhizus lipases by pichia pastoris and properties of the proteins. | the lipases of rhizopus spp. share a high 1,3-regiospecificity toward triacylglycerols, which makes them important enzymes in lipid modification. in the present study, the extracellularly active production of recombinant rhizopus arrhizuslipase was carried out with genes encoding the mature region (mral) and the mral having the prosequence (proral) in pichia pastoris. two transformed p. pastoris clones containing the multicopy of mral and proral genes were separately selected for the production ... | 2006 | 16382184 |
| an improved method for sn-2 position analysis of triacylglycerols in edible oils and fats based on immobilised lipase d (rhizopus delemar). | the use of lipase d (rhizopus delemar) immobilised on microporous polypropylene as a replacement for the standard pancreatic lipases used in the stereospecific sn-2 position analysis of triacylglycerols from edible oils and fats is studied. excellent hydrolysis characteristics are obtained in hexane/methanol solvents at reaction temperatures up to 60 degrees c with hydrolysis times of only 10-20 min. the favourable conditions for the hydrolysis reaction allow fats with higher melting points to b ... | 2006 | 16368101 |
| effect of the dates of extraction on the quality of potato pulp. | the objectives of this study were to evaluate the fundamental properties of potato pulp and to explore its potential uses. lactic acid bacteria were the dominant microbes in potato pulp over the season (maximum being 10(8)/g). the water content in potato pulp was approximately 80% in all season. starch and fiber were the main components of potato pulp, amounting to 80% of the dried matter regardless of the season. the fermentation of potato pulp by rhizopus oryzae progressed only in the potato p ... | 2006 | 16324844 |
| stabilization of the initial electrochemical potential for a metal-based potentiometric titration study of a biosorption process. | an interactive metal-based potentiometric titration method has been developed using an ion selective electrode for studying the sorption of metal cations. the accuracy of this technique was verified by analyzing the metal sorption mechanism for the biomass of rhizopus arrhizus fungus and diatomite, two dissimilar materials (organic and mineral, strong sorbent and weak sorbent) of a different order of cation exchange capacity. the problem of the initial electrochemical potential was addressed ide ... | 2006 | 16325652 |
| olive-mill wastewaters: a promising substrate for microbial lipase production. | the present study investigated the valorization of olive-mill wastewater (omw) by its use as a possible growth medium for the microbial production of extra-cellular lipase. to this end, strains of geotrichum candidum (nrrl y-552 and y-553), rhizopus arrhizus (nrrl 2286 and isrim 383), rhizopus oryzae (nrrl 6431), aspergillus oryzae (nrrl 1988 and 495), aspergillus niger (nrrl 334), candida cylindracea (nrrl y-17506) and penicillium citrinum (nrrl 1841 and 3754, isrim 118) were screened. all stra ... | 2006 | 16236495 |
| strain improvement for tannase production from co-culture of aspergillus foetidus and rhizopus oryzae. | spores from the co-culture of aspergillus foetidus and rhizopus oryzae were subjected to uv, heat and ntg (3-nitro,5-methylguanidine) mutagenesis. a few colonies were screened from the selected media for tannase study. amongst all, the best mutant isolated from the heat treatment (60 degrees c for 60 min) was scpr 337. the maximum yield of gallic acid and tannase in case of mutant strain was 95.2% and 53.6 u/ml with an incubation period of 30 h as compared to wild strain where the incubation per ... | 2006 | 15963714 |
| peripheral enzymatic deacetylation of chitin and reprecipitated chitin particles. | the enzymatic deacetylation of various chitin preparations was investigated using the fungal chitin deacetylase (cda) isolated from rhizopus oryzae growth medium. specific extracellular enzyme activity after solid state fermentation was 10 times higher than that after submerged fermentation. natural crystalline chitin is a very poor substrate for the enzyme, but showed a five-time better deacetylation after dissolution and reprecipitation. chitin particles, enzymatically deacetylated for only 1% ... | 2006 | 15919204 |
| chromium(vi) biosorption by dried rhizopus arrhizus: effect of salt (nacl) concentration on equilibrium and kinetic parameters. | some industrial wastewaters contain high quantities of salts besides heavy metal ions. the presence of salt ions leads to high ionic strength, which may significantly affect the performance of the biosorption process so the effect of salts on the biosorption of heavy metal ions should be investigated. in this study the biosorption of chromium(vi) from saline solutions on dried rhizopus arrhizus was studied as a function of ph, initial chromium(vi) and salt (nacl) concentrations in a batch system ... | 2007 | 17188810 |
| antimicrobial activity of methanol extract of origanum majorana l. (sweet marjoram). | in-vitro microbicidal activity of the methanol extract of origanum majorana l. was tested against seven fungi (fusarium solani, candida albicans, aspergillus niger, a. parasiticus, rhizopus oryzae, rhizoctonia otyzae-sativae and altemaria brassicicola) and six bacteria (bacillus subtilis, b. megaterium, escherichia coil, proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus). the methanol extract of o. majorana can be used as an effective herbal protectant against different pathogen ... | 2007 | 17718003 |
| role of the linker region in the expression of rhizopus oryzae glucoamylase. | rhizopus oryzae glucoamylase (roga) consists of three domains: an amino (n)-terminal raw starch-binding domain (sbd), a glycosylated linker domain, and a carboxy (c)-terminal catalytic domain. the 36-amino-acid linker region (residues 132-167) connects the two functional domains, but its structural and functional roles are unclear. | 2007 | 17593302 |
| early use of posaconazole in the successful treatment of rhino-orbital mucormycosis caused by rhizopus oryzae. | mucormycosis is a potentially fatal, rapidly destructive, opportunistic infection often seen in immunocompromsied individuals and patients with diabetes mellitus. we describe two patients with rhino-orbital mucormycosis caused by rhizopus oryzae who received posaconazole within 7 days of diagnosis and in whom cure was achieved in conjunction with extensive surgical resection of diseased tissue. posaconazole was well-tolerated. our experience indicates that this new extended-spectrum azole antifu ... | 2007 | 17624436 |
| transcriptional response of p. pastoris in fed-batch cultivations to rhizopus oryzae lipase production reveals upr induction. | the analysis of transcriptional levels of the genes involved in protein synthesis and secretion is a key factor to understand the host organism's responses to recombinant protein production, as well as their interaction with the cultivation conditions. novel techniques such as the sandwich hybridization allow monitoring quantitatively the dynamic changes of specific rnas. in this study, the transcriptional levels of some genes related to the unfolded protein response (upr) and central metabolism ... | 2007 | 17634115 |
| synthesis, cocrystallization, and enzymatic degradation of novel poly(butylene-co-propylene succinate) copolymers. | a series of poly(butylene-co-propylene succinate) (pbpsu) random copolyesters were synthesized and characterized using 1hnmr spectroscopy and viscometry. tensile properties decreased with increasing propylene succinate (psu) content. cocrystallization and multiple melting behaviors were investigated. the copolymers showed a eutectic behavior. the minimum melting point corresponded to 75 mol % psu. wide-angle x-ray diffraction (waxd) patterns showed that the copolymers with up to 60 mol % psu uni ... | 2007 | 17655353 |
| the iron chelator deferasirox protects mice from mucormycosis through iron starvation. | mucormycosis causes mortality in at least 50% of cases despite current first-line therapies. clinical and animal data indicate that the presence of elevated available serum iron predisposes the host to mucormycosis. here we demonstrate that deferasirox, an iron chelator recently approved for use in humans by the us fda, is a highly effective treatment for mucormycosis. deferasirox effectively chelated iron from rhizopus oryzae and demonstrated cidal activity in vitro against 28 of 29 clinical is ... | 2007 | 17786247 |
| cleavage of beta-carotene as the first step in sexual hormone synthesis in zygomycetes is mediated by a trisporic acid regulated beta-carotene oxygenase. | carotene cleavage is the necessary initial step in the biosynthesis of trisporic acid, the sexual signal in zygomycete fungi. two genes encoding putative carotene oxygenases, designated tsp3 and tsp4, were identified in the genome of the zygomycete rhizopus oryzae. using heterologous primers, tsp3 was cloned and sequenced also from blakeslea trispora. tsp3 transcription correlates with sexual development in both species. northern hybridization of b. trispora mrna revealed strong induction of tsp ... | 2007 | 17822929 |
| enhancement of the stability of a prolipase from rhizopus oryzae toward aldehydes by saturation mutagenesis. | a prolipase from rhizopus oryzae (prorol) was engineered in order to increase its stability toward lipid oxidation products such as aldehydes with the aim of improving its performance in oleochemical industries. out of 22 amino acid residues (15 lys and 7 his) prone to react with aldehydes, 6 lys and all his residues (except for the catalytic histidine) were chosen and subjected to saturation mutagenesis. in order to quickly and reliably identify stability mutants within the resulting libraries, ... | 2007 | 17890336 |
| fermentation as a bio-process to obtain functional soybean flours. | the effect of fermentation on the antioxidant compounds [vitamins c and e, total phenolic compounds (tpc), and reduced glutathione (gsh)], and antioxidant capacity [superoxide anion scavenging activity (sod-like activity), peroxyl radical-trapping capacity (prtc), inhibition of phosphatidylcholine (pc) peroxidation, and trolox equivalent antioxidant capacity (teac)] of soybean (glycine max cv. merit) was studied. fermentation was carried out in solid state in cracked seeds inoculated with asperg ... | 2007 | 17907774 |
| a rare case of mucormycosis of median sternotomy wound caused by rhizopus arrhizus. | we describe a case of mucormycosis of median sternotomy wound caused by rhizopus arrhizus . the patient, a known diabetic and a case of coronary artery disease underwent coronary artery bypass surgery. in the postoperative period, patient developed infection of the median sternotomy wound, from which r. arrhizus was isolated on culture. patient succumbed in spite of being treated with surgical debridement and amphotericin b. to the best of our knowledge, this is the first reported case of mucorm ... | 2007 | 18087101 |
| biochemical changes in micro-fungi fermented cassava flour produced from low- and medium-cyanide variety of cassava tubers. | comparative studies were carried out on the ability of pure strain of rhizopus oryzae and saccharomyces cerevisae to alter the nutritional quality of cassava flour produced from low- and medium-cyanide variety of cassava tuber. low- and medium-cyanide variety of cassava tubers were collected from international institute of tropical agriculture (iita), ibadan, nigeria. these tubers were washed, peeled, grated and aseptically inoculated with pure strains of rhizopus oryzae and saccharomyce cerevis ... | 2007 | 18087867 |
| rhizopus delemar is the proper name for rhizopus oryzae fumaric-malic acid producers. | the zygomycete rhizopus oryzae currently is identified by sporangiophore morphology and growth temperature, but heterogeneity of the species has been reported. we examined the suitability of organic acid production as an effective taxonomic character for reclassification of the species. strains were divided into two groups, la (lactic acid producer) and fma (fumaric-malic acid producers) according to organic acid production. these grouping were confirmed as phylogenetically distinct because anal ... | 2007 | 18268905 |
| optimization of lipase pretreatment prior to lipase immobilization to prevent loss of activity. | in our previous work, a method of pretreating lipase was developed to prevent loss of its activity during covalent immobilization. in this study, rhizopus oryzae lipase was pretreated before immobilization and then immobilized on a silica gel surface. the effects of the various materials and conditions used in the pretreatment stage on the activity of immobilized lipase were investigated. immobilized lipase pretreated with 0.1% of soybean oil had better activity than those pretreated with other ... | 2007 | 18051278 |
| studying pellet formation of a filamentous fungus rhizopus oryzae to enhance organic acid production. | using pelletized fungal biomass can effectively improve the fermentation performance for most of fugal strains. this article studied the effects of inoculum and medium compositions such as potato dextrose broth (pdb) as carbon source, soybean peptone, calcium carbonate, and metal ions on pellet formation of rhizopus oryzae. it has been found that metal ions had significantly negative effects on pellet formation whereas soybean peptone had positive effects. in addition pdb and calcium carbonate w ... | 2007 | 18478426 |
| biosorption of anionic textile dyes by nonviable biomass of fungi and yeast. | the nonviable biomass of aspergillus niger, aspergillus japonica, rhizopus nigricans, rhizopus arrhizus, and saccharomyces cerevisiae were screened for biosorption of textile dyes. the selected anionic reactive dyes were c.i. reactive black 8, c.i. reactive brown 9, c.i. reactive green 19, c.i. reactive blue 38, and c.i. reactive blue 3. experiments were conducted at initial dye concentration of 50, 100, 150 and 200mg/l. the effect of initial dye concentration, dose of biosorbent loading, temper ... | 2007 | 16997547 |
| production of lactic acid with loofa sponge immobilized rhizopus oryzae rbu2-10. | lactic acid production by rhizopus oryzae rbu2-10 immobilized in loofa sponge was evaluated. shape and texture of loofa sponge, which was obtained from the mature dried fruit of luffa cylindrica, remained intact after its treatment with buffers of varying ph and following its repeated autoclaving for up to four cycles (121 degrees c, 20 min per cycle). the medium having four pieces of loofa sponge (1.008 cm(3)) per 100 ml medium and inoculated with 3 x1 0(6) spores ml(-1) resulted maximum produc ... | 2007 | 16790343 |
| in vitro antimycotic activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. against opportunistic filamentous fungi. | xanthorrhizol was isolated from the rhizome of curcuma xanthorrhiza (zingiberaceae) and its in vitro activity against opportunistic filamentous fungi was evaluated using the nccls (m38-a) standard method. xanthorrhizol was found to be active against all the species tested, namely aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium oxysporum, rhizopus oryzae and trichophyton mentagrophytes: the mics being 2.0, 2.0, 2.0, 4.0, 1.0 and 1.0 microg/ml, while the mfcs were 4.0, 4.0, ... | 2007 | 17236167 |
| biotechnological production of lactic acid integrated with fishmeal wastewater treatment by rhizopus oryzae. | fishmeal wastewater, a seafood processing waste, was utilized for production of lactic acid and fungal biomass by rhizopus oryzae as 3.254 with the addition of sugars. the 30 g/l exogenous glucose in fishmeal wastewater was superior to starch in view of productivities of lactic acid and fungal biomass, and cod reduction. fishmeal wastewater can be a replacement for peptone which was the most suitable nitrogen source for lactic acid production among the tested organic or inorganic nitrogen source ... | 2007 | 17242928 |
| preparation of a whole-cell biocatalyst of mutated candida antarctica lipase b (mcalb) by a yeast molecular display system and its practical properties. | to prepare a whole-cell biocatalyst of a stable lipase at a low price, mutated candida antarctica lipase b (mcalb) constructed on the basis of the primary sequences of calbs from c. antarctica cbs 6678 strain and from c. antarctica lf 058 strain was displayed on a yeast cell surface by alpha-agglutinin as the anchor protein for easy handling and stability of the enzyme. when mcalb was displayed on the yeast cell surface, it showed a preference for short chain fatty acids, an advantage for produc ... | 2007 | 17262207 |
| development of an oat-based biorefinery for the production of l(+)-lactic acid by rhizopus oryzae and various value-added coproducts. | a novel oat-based biorefinery producing l(+)-lactic acid and various value-added coproducts (e.g., beta-glucan, anti-irritant solution) is proposed. pearling is employed for sequential separation of bran-rich fractions for the extraction of value-added coproducts. lactic acid production is achieved via fungal fermentation of rhizopus oryzae on pearled oat flour. maximum lactic acid concentration (51.7 g/l) and starch conversion yield (0.68 g/g) were achieved when an oat flour concentration of 11 ... | 2007 | 17288441 |
| primary subcutaneous zygomycosis due to rhizopus oryzae in a 71-year-old man with normal immune status. | a case of primary subcutaneous zygomycosis due to rhizopus oryzae is described in a 71-year-old man with normal immune status. diagnosis was based on histological examination and culture of biopsy fragments from skin lesions. the patient recovered after itraconazole therapy (200 mg day(-1) for 60 days followed by 100 mg day(-1) for 45 days). | 2007 | 17302755 |
| carbon assimilation profiles as a tool for identification of zygomycetes. | identification of zygomycetes is difficult and time-consuming by standard microbiological procedures. carbon assimilation profiles are commonly used for yeast-and bacterial-species identification but rarely for filamentous-fungus identification. carbon assimilation profiles were evaluated using the commercialized kits id32c and api 50 ch, which contain 31 and 49 tests, respectively, to serve as simple tools for species identification of zygomycetes in clinical microbiology laboratories. fifty-se ... | 2007 | 17329449 |
| mucormycosis of the upper extremity in a patient with alcoholic encephalopathy. | cutaneous mucormycosis is a rare opportunistic infection caused by fungi of the class zygomycetes that can be rapidly fatal if unrecognized. the diagnosis of this infection is often made by infectious disease, dermatologic, or intensive care specialists. lesions that affect the upper limb may require a hand surgeon to diagnose the infection. the diagnosis may be difficult to make, because these infectious lesions can be confused with ischemic pathology. we report on a rare case of cutaneous muco ... | 2007 | 17336848 |
| construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. | we determined whether the cocultivation of yeast cells displaying a zz-domain and secreting an fc fusion protein can be a novel tool for the recovery of secreted recombinant proteins. the zz-domain from staphylococcus aureus protein a was displayed on the cell surface of saccharomyces cerevisiae under the control of the gal1 promoter. strain s. cerevisiae by4742 cells displaying the zz-domain on their surface were used for cocultivation with cells that produce a target protein fused to the fc fr ... | 2007 | 17345082 |
| sorbitol co-feeding reduces metabolic burden caused by the overexpression of a rhizopus oryzae lipase in pichia pastoris. | to improve the specific production rate of rhizopus oryzae lipase (rol) in pichia pastoris, a protein that triggers the unfolded protein response in p. pastoris, the effect of sorbitol/methanol mixed substrates was tested in batch and fed-batch cultures. remarkably, a different substrate consumption behaviour was observed depending on the host's phenotype (mut(+) or mut(s)) in batch cultures: when the methanol assimilation capacity is genetically reduced (mut(s) phenotype), both substrates were ... | 2007 | 17399833 |
| stable and continuous long-term enzymatic reaction using an enzyme-nanofiber composite. | this study shows the preparation and application of enzyme-nanofiber composites for long-term stable operation. the enzyme-nanofiber composite was prepared by coating an enzyme aggregate, the esterase from rhizopus oryzae, on the surface of the nanofibers. after immobilization on the nanofiber, the apparent k ( m ) for the immobilized esterase was 1.48-fold higher than that of the free esterase, with values of 0.98 and 1.35 mm for the free and immobilized enzymes, respectively. it was found that ... | 2007 | 17404727 |
| production of butyl acetate ester by lipase from novel strain of rhizopus oryzae. | a new lipase preparation from rhizopus oryzae was used to catalyze the esterification reaction between acetic acid and butanol to produce butyl acetate ester (pineapple flavor). this flavor compound can be used in food, cosmetic and pharmaceutical industries. only 3% of butyl acetate was obtained when free lipase was used in the synthesis containing only the substrates. in contrast, the conversion yield reached 25% when immobilized lipase was used under the same conditions. the synthesis of buty ... | 2007 | 17502279 |
| isolation and characterization of two genes that encode active glucoamylase without a starch binding domain from rhizopus oryzae. | glucoamylase obtained from rhizopus sp. is frequently preferred for certain applications of starch modification or saccharification. the predominant enzyme, which contains a starch-binding domain on the amino terminus, has been previously characterized from several species. additionally, the cdna encoding this protein was cloned and found to show 100% identity to a r. oryzae strain 99-880 that has recently been sequenced by the broad institute of harvard and massachusetts institute of technology ... | 2007 | 17503147 |
| rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in pichia pastoris. | together with the development of optical sensors, fluorometry is becoming an increasingly attractive tool for the monitoring of cultivation processes. in this context, the green fluorescence protein (gfp) has been proposed as a molecular reporter when fused to target proteins to study their subcellular localization or secretion behaviour. the present work evaluates the use of the gfp fusion partner for monitoring extracellular production of a rhizopus oryzae lipase (rol) in pichia pastoris by me ... | 2007 | 17511861 |
| production of a rhizopus oryzae lipase from pichia pastoris using alternative operational strategies. | different cultivation strategies have been compared for the production of rhizopus oryzae lipase (rol) from pichia pastoris. several drawbacks have been found using a methanol non-limited fed-batch. on the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. a temperature limited fed-batch has been proposed to solve both problems. however, in our case study a methanol non-limited fed-batch results in better productivities. finally, ... | 2007 | 17544535 |
| native and modified lactate dehydrogenase expression in a fumaric acid producing isolate rhizopus oryzae 99-880. | rhizopus oryzae is subdivided into two groups based on genetic and phenotypic differences. type-i isolates accumulate primarily lactic acid when grown in the presence of a fermentable carbon source and contain two lactate dehydrogenase genes, ldha and ldhb. type-ii isolates synthesize predominantly fumaric acid and only have an ldhb gene. in this study, we determined that ldhb transcript is only minimally expressed in the type-ii isolate r. oryzae 99-880. ldhb enzyme purified from gene clones is ... | 2007 | 17551728 |
| posaconazole prophylaxis in experimental systemic zygomycosis. | three isolates of zygomycetes belonging to two different genera (rhizopus oryzae and absidia corymbifera) were used to produce a systemic infection in neutropenic mice. on days -2 and -1 and at 2 h prior to infection, the mice received either posaconazole (pos) at doses ranging from 20 to 80 mg/kg of body weight/day or amphotericin b (amb) at 1 mg/kg/day. antifungal drug efficacy was assessed by determination of the prolongation of survival, determination of the percentage of infected organs (br ... | 2007 | 17060525 |
| continuous fixed bed biosorption of reactive dyes by dried rhizopus arrhizus: determination of column capacity. | a continuous fixed bed study was carried out by using dried rhizopus arrhizus as a biosorbent for the removal of three reactive dyes; gemacion (procion) red h-e7b (gr), a monoclorotriazine mono-azo type reactive dye; gemazol turquise blue-g (gtb), a vinyl sulfone mono-azo type reactive dye and gemactive (reactive) black hfgr (gb), a vinyl sulfone di-azo type reactive dye from aqueous solution. the effect of operating parameters such as flow rate and inlet dye concentration on the sorption charac ... | 2007 | 17070992 |
| application of response surface methodology for the biosorption of copper using rhizopus arrhizus. | response surface methodology was used to study the cumulative effect of the various parameters namely, initial copper ion concentration, ph, temperature, biomass loading and to optimize the process conditions for the maximum removal of copper. for obtaining the mutual interaction between the variables and optimizing these variables, a 2(4) full factorial central composite design using response surface methodology was employed. the analysis of variance (anova) of the quadratic model demonstrates ... | 2007 | 17084526 |
| combining regio- and enantioselectivity of lipases for the preparation of (r)-4-chloro-2-butanol. | preparation of 98% ee (r)-4-chloro-2-butanol was carried out by the enzymatic hydrolysis of chlorohydrin esters, using fungal resting cells and commercial enzymes. hydrolyzes were carried out using lipases from candida antarctica (novozym 435), c. rugosa, rhizomucor miehei (lipozyme im), burkolia cepacia, and resting cells of rhizopus oryzae and aspergillus flavus. the influence of the enzyme, the solvent, the temperature, and the alkyl chain length on the selectivity of hydrolyzes of isomeric m ... | 2007 | 17089342 |