Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| development of an isothermoal amplification-based assay for rapid visual detection of an orf virus. | orf virus (orfv) is the causative agent of a severe infectious skin disease (also known as contagious ecthyma) in goats, sheep and other small ruminants. importantly, orfv also infect humans which causes a public health concern in the context of changing environment and increase in human populations. the rapid detection is critical in effective control of the disease and urgently needed. | 2016 | 26993468 |
| detection of peste des petits ruminants virus and goatpox virus from an outbreak in goats with high mortality in meghalaya state, india. | we describe a laboratory investigation carried out to confirm the etiology of the heavy mortality (37 animals died out of total 44, i.e. 84%) in goats in ri-bhoi district of meghalaya, northeast region of india in december 2015. the clinical signs observed were abortion, diarrhea, high fever (up to 104°f), pox lesion in the skin, and respiratory distress. | 2016 | 27733807 |
| pastoralism in the drylands of latin america: argentina, chile, mexico and peru. | this article discusses various aspects of pastoralism in the latin american countries with the largest dryland areas. the topics covered include: social, economic and institutional issues; grasslands and their carrying capacity; production systems and productivity rates; competition for forage resources between domestic livestock and wildlife; and the health status of livestock and wildlife. most grasslands exhibit some degree of degradation. the percentage of offspring reaching weaning age is l ... | 2016 | 27917972 |
| history and current status of peste des petits ruminants virus in tanzania. | peste des petits ruminants virus (pprv) causes the acute, highly contagious disease peste des petits ruminants (ppr) that affects small domestic and wild ruminants. ppr is of importance in the small livestock-keeping industry in tanzania, especially in rural areas as it is an important source of livelihood. morbidity and case fatality rate can be as high as 80-100% in naïve herds; however, in endemic areas, morbidity and case fatality range between 10 and 100% where previous immunity, age, and s ... | 2016 | 27770516 |
| development of an immunoperoxidase monolayer assay for the detection of antibodies against peste des petits ruminants virus based on bhk-21 cell line stably expressing the goat signaling lymphocyte activation molecule. | from 2013 to 2015, peste des petits ruminants (ppr) broke out in more than half of the provinces of china; thus, the application and development of diagnostic methods are very important for the control of ppr. here, an immunoperoxidase monolayer assay (ipma) was developed to detect antibodies against ppr. however, during ipma development, we found that vero cells were not the appropriate choice because staining results were not easily observed. therefore, we first established a baby hamster kidn ... | 2016 | 27768770 |
| comparison of nucleotide sequences of recent and previous lineages of peste-des-petits-ruminants viruses of sheep and goats in nigeria. | peste-des-petits-ruminants virus (pprv) is a highly contagious, fatal and economically important viral disease of small ruminants that is still endemic and militates against the production of sheep and goats in endemic areas of the world. the aim of this study was to describe the viral strains within the country. this was carried out by collecting tissue and swab samples from sheep and goats in various agro-ecological zones of nigeria. the phylogeny of archived pprv strains or isolates and those ... | 2016 | 27608505 |
| modelling the dynamics of post-vaccination immunity rate in a population of sahelian sheep after a vaccination campaign against peste des petits ruminants virus. | peste des petits ruminants (ppr) is an acute infectious viral disease affecting domestic small ruminants (sheep and goats) and some wild ruminant species in africa, the middle east and asia. a global ppr control strategy based on mass vaccination-in regions where ppr is endemic-was recently designed and launched by international organizations. sahelian africa is one of the most challenging endemic regions for ppr control. indeed, strong seasonal and annual variations in mating, mortality and off ... | 2016 | 27603710 |
| corynebacterium cutis lysate treatment can increase the efficacies of ppr vaccine. | this study aimed to evaluate the effects of peste des petits ruminants (ppr) vaccine on cytokine and antibody levels in sheep when administered alone or in combination with corynebacterium cutis lysate (ccl). the ppr vaccine group received a single subcutaneous axillary injection of the ppr vaccine (1 ml containing tissue culture infectious dose (tcid)50 attenuated live pprv, n = 6) and the combination treatment (1 ml ccl and 1 ml ppr vaccine, n = 6) groups received a single subcutaneous axillar ... | 2016 | 27533481 |
| one-step multiplex rt-qpcr assay for the detection of peste des petits ruminants virus, capripoxvirus, pasteurella multocida and mycoplasma capricolum subspecies (ssp.) capripneumoniae. | respiratory infections, although showing common clinical symptoms like pneumonia, are caused by bacterial, viral or parasitic agents. these are often reported in sheep and goats populations and cause huge economic losses to the animal owners in developing countries. detection of these diseases is routinely done using elisa or microbiological methods which are being reinforced or replaced by molecular based detection methods including multiplex assays, where detection of different pathogens is ca ... | 2016 | 27123588 |
| detection of peste des petits ruminants virus (pprv) genome from nasal swabs of dogs. | peste des petits ruminants virus (pprv) one of the most important viruses of small ruminants has a restricted host range. we report here the presence of pprv virus in the nasal swabs of 3 out of 12 dogs in a routine microarray screening. the presence of pprv sequence was further confirmed by pcr and sequencing. the sequence analysis revealed that the pprv virus has close similarities with the viruses present in indian subcontinent but was not identical to the vaccine virus used in india. results ... | 2016 | 27040823 |
| molecular evolution and characterization of hemagglutinin (h) in peste des petits ruminants virus. | peste des petits ruminants (ppr) is an acute, highly contagious, and febrile viral disease that affects both domestic and wild small ruminants. the disease has become a major obstacle to the development of sustainable agriculture. hemagglutinin (h), the envelope glycoprotein of peste des petits ruminants virus (pprv), plays a crucial role in regulating viral adsorption and entry, thus determining pathogenicity, and release of newly produced viral particles. in order to accurately understand the ... | 2016 | 27035347 |
| major mutation events in structural genes of peste des petits ruminants virus through serial passages in vitro. | peste des petits ruminants (ppr) is an highly contagious disease of small ruminants, and caused by peste des petits ruminants virus (pprv), a member of the genus morbillivirus in the family paramyxoviridae. the first outbreak of ppr in china was officially reported in july 2007, when a pprv strain was successfully isolated from a sick goat in tibet, followed by sequencing at a full-genome level (china/tibet/geg/07-30, genbank: fj905304.1). to date, this isolate has been virulently attenuated by ... | 2016 | 26995222 |
| protection of cattle against rinderpest by vaccination with wild-type but not attenuated strains of peste des petits ruminants virus. | although rinderpest virus (rpv) has been eradicated in the wild, efforts are still continuing to restrict the extent to which live virus is distributed in facilities around the world and to prepare for any reappearance of the disease, whether through deliberate or accidental release. in an effort to find an alternative vaccine which could be used in place of the traditional live attenuated rpv strains, we have determined whether cattle can be protected from rinderpest by inoculation with vaccine ... | 2016 | 26984722 |
| serosurvey of peste des petits ruminants virus in small ruminants from different agro-ecological zones of nigeria. | peste des petits ruminants, caused by the peste des petits ruminants virus (pprv), is a highly contagious and economically important transboundary viral disease of domestic and wild small ruminants and a major hindrance to small-ruminant production in nigeria. the seroprevalence and distribution of pprv antibodies in small ruminants in rural households, farms, live animal markets and slaughter slabs across the six different agro-ecological zones of nigeria were determined. a total of 4548 serum ... | 2016 | 26974177 |
| the global eradication of peste des petits ruminants (ppr) within 15 years--is this a pipe dream? | gordon scott did much to inspire the eradication of rinderpest, probably the most outstanding veterinary achievement of the twentieth century, and one currently inspiring is the proposed eradication of peste des petits ruminants (ppr), a disease of sheep and goats caused by a closely related morbillivirus. the evolution and geographic spread of ppr along with its epidemiological characteristics are discussed and related to ways in which its spread can be reduced through the provision of diagnost ... | 2016 | 26851956 |
| peste des petits ruminants in benin: persistence of a single virus genotype in the country for over 42 years. | peste des petits ruminants (ppr) is a contagious and often fatal disease affecting sheep and goats. currently, it is endemic in africa, the middle and near east, the indian subcontinent and china. understanding the molecular epidemiology and evolution of ppr virus (pprv) can assist in the control of the transboundary spread of this economically important disease. we isolated pprv from pathological and swab samples collected 42 years apart (1969 and 2011) in benin, west africa, and sequenced the ... | 2016 | 26801518 |
| peste des petits ruminants diagnosis and diagnostic tools at a glance: perspectives on global control and eradication. | peste des petits ruminants (ppr) is a highly contagious, economically important viral disease of small ruminants, targeted for global eradication by the year 2030. the recent geographic surge in ppr virus distribution, economic implications, the success of the rinderpest eradication campaign, and ongoing national/regional efforts convinced the fao and oie to initiate a global ppr control and eradication strategy. since its discovery, a series of diagnostic tools have been developed for detecting ... | 2016 | 27522587 |
| assessment of immune response to a lyophilized peste-des-petits-ruminants virus vaccine in three different breeds of goats. | immune response to a lyophilized peste-des-petits-ruminants virus (pprv) vaccine was evaluated in three different breeds of goats. | 2016 | 27397978 |
| preliminary results on innocuity and immunogenicity of an inactivated vaccine against peste des petits ruminants. | peste des petits ruminants (ppr) virus belongs to the family paramyxoviridae and represents a major threat to small livestock industry. in recent years, outbreaks of ppr have occurred in turkey and north africa. in endemic areas, disease prevention is accomplished using live‑attenuated vaccines. however, the use of live vaccines in non‑endemic regions, such as europe, is not approved by veterinary authorities. in these regions inactivated vaccines are then the only viable alternative. in this st ... | 2016 | 27393872 |
| sequence analysis of peste des petits ruminants virus from ibexes in xinjiang, china. | peste des petits ruminants (ppr) is an infectious disease caused by peste des petits ruminants virus (pprv). while ppr mainly affects domestic goats and sheep, it also affects wild ungulates such as ibex, blue sheep, and gazelle, although there are few reports regarding pprv infection in wild animals. between january 2015 and february 2015, it was found for the first time that wild ibexes died from pprv infection in bazhou, xinjiang, china, where a total of 38 ibexes (including young and adult i ... | 2016 | 27323119 |
| first report and characterization of peste des petits ruminants virus in liberia, west africa. | 2016 | 27315206 | |
| rapid detection of lineage iv peste des petits ruminants virus by real-time rt-pcr. | peste des petits ruminants virus (pprv) is the cause agent of peste des petitis ruminants (ppr). a novel lineage iv pprv has reemerged in china in 2013 and 2014. mass vaccination was implemented in most provinces in china. in order to detect lineage iv pprv in clinical samples and to distinguish rapidly it from the other lineages pprvs, a real-time rt-pcr assay was developed. this assay showed high sensitivity, specificity and efficiency in differentiating the lineage iv pprv from others. the pe ... | 2016 | 27260657 |
| the opportunity to eradicate peste des petits ruminants. | peste des petits ruminants (ppr) is a highly infectious disease of sheep and goats that is caused by ppr virus, a member of the genus morbillivirus that includes the viruses that cause rinderpest (rp) in cattle. rp was the first animal disease to be globally eradicated in 2011 and is only the second disease, after smallpox, to have ever been eradicated. ppr is one of the principal constraints to small ruminant production in africa, asia, and the middle east. the epidemiology of ppr and rp as wel ... | 2016 | 27183645 |
| the first report of peste des petits ruminants (ppr) in camels (camelus dromedarius) in iran. | in mid-july 2013, an outbreak of peste des petits ruminants (ppr) was observed in a herd of camels after they were imported from kuwait to the khuzestan province in southwest of iran. the clinical signs of the affected animals included sudden death, fever, oral erosion, and ecthyma like lesions, yellowish diarrhea, pneumonia and respiratory distress, enlargement of lymph node, severe dehydration, dermatitis, ulcerative keratitis, and conjunctivitis. necropsy findings included keratoconjunctiviti ... | 2016 | 27155951 |
| peste des petits ruminants virus. | peste des petits ruminants virus (pprv) causes a severe contagious disease of sheep and goats and has spread extensively through the developing world. because of its disproportionately large impact on the livelihoods of low-income livestock keepers, and the availability of effective vaccines and good diagnostics, the virus is being targeted for global control and eventual eradication. in this review we examine the origin of the virus and its current distribution, and the factors that have led in ... | 2016 | 27112279 |
| re-emergence of peste des petits ruminants virus in 2015 in morocco: molecular characterization and experimental infection in alpine goats. | peste des petits ruminants (ppr) is a transboundary viral disease of small ruminants that causes huge economic losses in africa, the middle east and asia. in morocco, the first ppr outbreak was notified in 2008. since then no cases were reported for seven years, probably due to three successive vaccination campaigns during 2008-2011 and close surveillance at the border areas. in june 2015, the disease re-emerged in morocco, raising questions about the origin of the virus. the ppr virus was confi ... | 2016 | 27938675 |
| determination of the minimum fully protective dose of adenovirus-based diva vaccine against peste des petits ruminants virus challenge in east african goats. | peste des petits ruminants virus (pprv) causes an economically important disease of sheep and goats, primarily in developing countries. it is becoming the object of intensive international control efforts. current vaccines do not allow vaccinated and infected animals to be distinguished (no diva capability). we have previously shown that recombinant, replication-defective, adenovirus expressing the pprv h glycoprotein (adh) gives full protection against wild type pprv challenge. we have now test ... | 2016 | 26796101 |
| veronectin-4 is a highly sensitive cell line that can be used for the isolation and titration of peste des petits ruminants virus. | peste des petits ruminants virus (pprv) is a member of the morbillivirus subgroup of the family paramyxoviridae, and is one of the most contagious diseases of small ruminants throughout africa and the rest of the world. different cell lines have previously been used to isolate pprv but with limited success. thus, to improve the isolation of morbilliviruses, human, canine, and goat homologues of the lymphocyte receptor signaling lymphocyte activation molecule (slam) have been introduced into cell ... | 2016 | 26615804 |
| peste des petits ruminants viruses re-emerging in china, 2013-2014. | re-emergence of peste des petits ruminants (ppr) was officially reported in xinjiang uygur autonomous region in north-western china in november 2013, and then along with the movements of goats and sheep, this disease rapidly spread to other provinces, autonomous regions and municipalities (p/a/m) of china. a total of 256 ppr-affected counties in 22 p/a/m were identified up to september 2014. phylogenetic analysis revealed that the current circulating strains and tibet strains isolated previously ... | 2016 | 25620455 |
| evaluation of humoral response and protective efficacy of an inactivated vaccine against peste des petits ruminants virus in goats. | four goats were inoculated with an inactivated peste des petits ruminants virus (pprv) vaccine. three unvaccinated goats were kept as controls. after 36 days, the four goats were revaccinated. the immune response was monitored by virus neutralization test showing that two doses of the vaccine were able to stimulate strong immune response in all the vaccinated animals. the vaccinated goat and the controls were challenged with virulent pprv intranasally. after pprv challenge, the three control goa ... | 2016 | 25594237 |
| emergence of lineage iv peste des petits ruminants virus in ethiopia: complete genome sequence of an ethiopian isolate 2010. | isolates of peste des petits ruminants virus (pprv) can be segregated genetically into four lineages. for decades, lineages i-iii have been reported across africa whilst lineage iv has predominantly circulated across asia. however, the lineage distribution is currently changing in africa. importantly, full genome sequence data for african field isolates have been lacking. here, we announce the first complete genome sequence of a field isolate of peste des petits ruminants virus (pprv) from east ... | 2016 | 25400010 |
| companion animals as a source of viruses for human beings and food production animals. | companion animals comprise a wide variety of species, including dogs, cats, horses, ferrets, guinea pigs, reptiles, birds and ornamental fish, as well as food production animal species, such as domestic pigs, kept as companion animals. despite their prominent place in human society, little is known about the role of companion animals as sources of viruses for people and food production animals. therefore, we reviewed the literature for accounts of infections of companion animals by zoonotic viru ... | 2016 | 27522300 |
| comparative and temporal transcriptome analysis of peste des petits ruminants virus infected goat peripheral blood mononuclear cells. | peste des petits ruminanats virus (pprv), a morbillivirus causes an acute, highly contagious disease - peste des petits ruminants (ppr), affecting goats and sheep. sungri/96 vaccine strain is widely used for mass vaccination programs in india against ppr and is considered the most potent vaccine providing long-term immunity. however, occurrence of outbreaks due to emerging ppr viruses may be a challenge. in this study, the temporal dynamics of immune response in goat peripheral blood mononuclear ... | 2017 | 28017736 |
| detection and genome analysis of a lineage iii peste des petits ruminants virus in kenya in 2011. | in may 2011 in turkana county, north-western kenya, tissue samples were collected from goats suspected of having died of peste des petits ruminant (ppr) disease, an acute viral disease of small ruminants. the samples were processed and tested by reverse transcriptase pcr for the presence of ppr viral rna. the positive samples were sequenced and identified as belonging to peste des petits ruminants virus (pprv) lineage iii. full-genome analysis of one of the positive samples revealed that the vir ... | 2017 | 25962318 |
| serological detection of antibodies to peste des petits ruminants virus in large ruminants. | peste des petits ruminants (ppr) is an economically important disease of small ruminants with a rapidly expanding geographical distribution. peste des petits ruminants virus may manifest in a variety of ways with disease ranging from acute to subclinical. we investigated the exposure of large ruminants to pprv in areas where the virus is endemic in the small ruminant population by assessing the serological status of groups of animals. this study focused on the punjab province of pakistan as an a ... | 2017 | 26200233 |
| m protein is sufficient for assembly and release of peste des petits ruminants virus-like particles. | peste des petits ruminants virus (pprv), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (m), nucleocapsid proteins (n), fusion protein (f) and hemagglutinin protein (h)) and could cause high morbidity and mortality in sheep and goats. although a vaccine strain of pprv has been rescued and co-expression of m and n could yield pprv-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. in this study, pl ... | 2017 | 28330747 |
| peste des petits ruminants virus in vulnerable wild small ruminants, iran, 2014-2016. | in 2014-2016, >1,000 wild goats and sheep in 4 northern and central provinces of iran died from peste des petits ruminants virus (pprv) infection. partial nucleoprotein sequencing of pprv from 3 animals showed a close relationship to lineage 4 strains from china. control measures are needed to preserve vulnerable ruminant populations. | 2017 | 28322692 |
| peste des petits ruminants infection in domestic ruminants in sudan. | the existence of peste des petits ruminants (ppr) in domestic ruminants and camels in sudan during 2008-2012 was investigated. lung tissues and serum samples were randomly collected from sheep, goats, cattle, and camels at different areas of sudan. a total of 12,384 serum samples were collected from clinically healthy 7413 sheep, 1988 camels, 1501 cattle, 1459 goats, and 23 gazelles at different areas in the sudan. they were examined for ppr antibodies using competitive elisa (celisa). the overa ... | 2017 | 28321790 |
| ovar-mhc polymorphism in malpura and avikalin sheep vaccinated for peste des petits ruminants (ppr) virus. | india harbors a vast diversity of sheep (40 breeds). the study was carried out to assess the genetic diversity of drb1 and dqa2 locus of the ovar-mhc and their possible association with peste des petits ruminants (ppr) virus vaccine response in malpura and avikalin sheep breeds maintained at an organized institute flock in the semi-arid region of india. genetic analysis revealed the rich diversity of drb1 locus with 23 alleles in malpura and 21 alleles in avikalin sheep that included 9 new allel ... | 2017 | 28267411 |
| genome sequencing of an indian peste des petits ruminants virus isolate, izatnagar/94, and its implications for virus diversity, divergence and phylogeography. | peste des petits ruminants is an important transboundary disease infecting small ruminants. genome or gene sequence analysis enriches our knowledge about the evolution and transboundary nature of the causative agent of this disease, peste des petits ruminants virus (pprv). although analysis using whole genome sequences of pathogens leads to more precise phylogenetic relationships, when compared to individual genes or partial sequences, there is still a need to identify specific genes/genomic reg ... | 2017 | 28247095 |
| genetic detection of peste des petits ruminants virus under field conditions: a step forward towards disease eradication. | the devastating viral disease of small ruminants namely peste des petits ruminants (ppr) declared as target for "global eradication" in 2015 by the food and agriculture organization (fao) and the world organization for animal health (oie). for a successful eradication campaign, molecular diagnostic tools are preferred for their specificity, efficacy and robustness to compliment prophylactic measures and surveillance methods. however, molecular tools have a few limitations including, costly equip ... | 2017 | 28122564 |
| diagnosis of peste des petits ruminants in wild and domestic animals in xinjiang, china, 2013-2016. | peste des petits ruminants viruses (pprvs) re-emerged in china at the end of 2013 and then spread rapidly into 22 provinces through movement of live goats and sheep. in this study, 96 samples of domestic animals and 13 samples of wildlife were analysed for the presence of pprv infection by elisa or rt-pcr. of 96 samples from sheep and goats, 91 were pprv positive, whereas all of the 13 samples from three wild species, capra ibex (capra ibex sibirica), argali (ovis ammon) and goitered gazelle (ga ... | 2017 | 28101989 |
| outbreak of foot and mouth disease and peste des petits ruminants in sheep flock imported for immediate slaughter in riyadh. | to detect and identify the causative agent or agents of the following clinical symptoms which were fever, lack of appetite, salivation, vesiculation, erosions of the buccal mucosa, nose, and feet. the signs vary from mild to severe. the mortality rate of the disease is high. the morbidity rate reaches up to 100%. sheep also show bloody diarrhea and rapid respiration. sheep flock resident in el-kharje governorate. | 2017 | 28344409 |
| development of real-time and lateral flow strip reverse transcription recombinase polymerase amplification assays for rapid detection of peste des petits ruminants virus. | peste des petits ruminants (ppr) is an economically important, office international des epizooties (oie) notifiable, transboundary viral disease of small ruminants such as sheep and goat. ppr virus (pprv), a negative-sense single-stranded rna virus, is the causal agent of ppr. therefore, sensitive, specific and rapid diagnostic assay for the detection of pprv are necessary to accurately and promptly diagnose suspected case of ppr. | 2017 | 28173845 |
| eradication of transboundary animal diseases: can the rinderpest success story be repeated? | a matrix system was developed to aid in the evaluation of the technical amenability to eradication, through mass vaccination, of transboundary animal diseases (tads). the system involved evaluation of three basic criteria - disease management efficiency, surveillance and epidemiological factors - each in turn comprised of a number of elements (17 in all). on that basis, 25 tads that have occurred or do occur in southern africa and for which vaccines are available, in addition to rinderpest (inco ... | 2017 | 26104982 |
| concurrent infection of bluetongue and peste-des-petits-ruminants virus in small ruminants in haryana state of india. | bluetongue (bt) and peste-des-petits-ruminants (ppr) are major transboundary diseases of small ruminant, which are endemic in india. testing of bluetongue virus (btv) and peste-des-petits-ruminants virus (pprv) from recent outbreaks (2015-2016) in different regions of haryana state of india revealed that 27.5% of the samples showed the presence of dual infection of btv and pprv. analysis of seg-2 of btv (the serotype-determining protein) showed the presence of btv-12w in several isolates. howeve ... | 2017 | 28116836 |
| development of multiplex pcr for simultaneous detection and differentiation of six dna and rna viruses from clinical samples of sheep and goats. | multiplex reverse transcription-polymerase chain reaction (rt-pcr) and pcr protocols were developed and subsequently evaluated for its effectiveness in detecting simultaneously single and mixed infections in sheep and goats. specific primers for three dna viruses and three rna viruses, including foot and mouth disease virus (fmdv), bluetongue virus (btv), peste des petits ruminants virus (pprv), sheeppox virus (sppv), goatpox virus (gtpv) and orf virus (orfv) were used for testing procedure. a s ... | 2017 | 28111057 |
| a new immunoassay of serum antibodies against peste des petits ruminants virus using quantum dots and a lateral-flow test strip. | a fast and ultrasensitive test-strip system combining quantum dots (qds) with a lateral-flow immunoassay strip (lfias) was established for detection of peste des petits ruminants virus (pprv) antibody. the highly luminescent water-soluble carboxyl-functionalized qds were used as the signal output and were conjugated to streptococcal protein g (spg), which was capable of binding to immunoglobulin g (igg) from many species through an amide bond to capture the target pprv iggs. the pprv n protein, ... | 2017 | 27783124 |
| modulation of host mirnas transcriptome in lung and spleen of peste des petits ruminants virus infected sheep and goats. | peste des petits ruminants (ppr) is one of the highly contagious viral disease, characterized by fever, sore mouth, conjunctivitis, gastroenteritis, and pneumonia, primarily affecting sheep and goats. reports suggested variable host response in goats and sheep and this host response vis-a-vis the expression of micrornas (mirnas) has not been investigated. here, mirnas were sequenced and proteomics data were generated to identify the role of differentially expressed mirna (demirna) in ppr virus ( ... | 2017 | 28694795 |
| first genetic characterization of peste des petits ruminants virus from mongolia. | between august and september 2016 pathological samples were collected from sheep and goats following suspected peste des petits ruminants (ppr) outbreaks in western mongolia. rt-pcr followed by sequencing and phylogenetic analysis of the samples confirmed the presence of a ppr virus belonging to lineage iv. a full genome analysis of the viral rna from one of the samples revealed a high similarity (99.0-99.5%) with ppr viruses currently circulating in china (2013-2015) indicating a common origin. ... | 2017 | 28667443 |
| a thermostable presentation of the live, attenuated peste des petits ruminants vaccine in use in africa and asia. | the research objective was to develop a thermostable vaccine against peste des petits ruminants (ppr), a morbilliviral disease of small ruminants targeted for eradication that is a major constraint on the livelihoods of the rural poor throughout much of africa and asia. although existing ppr vaccines provide life-long immunity, they require continuous refrigeration. this limits their utility in developing countries. methods for the lyophilization of a related morbillivirus, rinderpest (rp), resu ... | 2017 | 28566253 |
| persistence of lineage iv peste-des-petits ruminants virus within israel since 1993: an evolutionary perspective. | peste-des-petits ruminants (ppr) is one of the most important infectious diseases of domesticated small ruminants. from the initial identification in 1942 in west africa, ppr virus (pprv) has spread throughout much of the developing world. pprv is now considered endemic throughout africa, with the notable exception of south africa, the middle-east and israel, as well as south-, east-, and central asia. despite this widespread dispersal, the evolution and transmission of pprv in endemic populatio ... | 2017 | 28545149 |
| control of the induction of type i interferon by peste des petits ruminants virus. | peste des petits ruminants virus (pprv) is a morbillivirus that produces clinical disease in goats and sheep. we have studied the induction of interferon-β (ifn-β) following infection of cultured cells with wild-type and vaccine strains of pprv, and the effects of such infection with pprv on the induction of ifn-β through both mda-5 and rig-i mediated pathways. using both reporter assays and direct measurement of ifn-β mrna, we have found that pprv infection induces ifn-β only weakly and transie ... | 2017 | 28475628 |
| peste des petits ruminants (ppr): a neglected tropical disease in maghreb region of north africa and its threat to europe. | peste des petits ruminants (ppr) is a contagious disease listed by the world organisation for animal health (oie) as being a specific hazard. it affects sheep, goats, and wild ungulates, and is prevalent throughout the developing world particularly asia, the middle east, and africa. ppr has been targeted for eradication by 2030 by the food and agriculture organization of the united nations (fao) and the oie, after the successful eradication of the related disease, rinderpest in cattle. ppr was f ... | 2017 | 28426782 |
| peste des petits ruminants threatens endangered antelope population. | 2017 | 28154210 | |
| peste des petits ruminants risk factors and space-time clusters in mymensingh, bangladesh. | using a hospital-based case-control study design, our aim was to identify risk factors for-and space-time clusters of-peste des petits ruminants (ppr) in mymensingh, bangladesh. three hundred and eighty ppr cases diagnosed between january 2005 and december 2014 at the bangladesh agricultural university veterinary teaching hospital (bauvth) were selected; three controls per case from bauvth were then selected (n = 1,048). from records, data extracted included information on date of report, locati ... | 2017 | 28109070 |
| 'huge concern' about increased risk of peste des petits ruminants entering europe. | 2017 | 28522624 | |
| advances in peste des petits ruminants vaccines. | peste des petits ruminants (ppr) is a highly contagious disease of small ruminants that leads to high morbidity and mortality thereby results in devastating economic consequences to the livestock industry. ppr is currently endemic across most parts of asia and africa, the two regions with the highest concentration of poor people in the world. sheep and goats in particularly contribute significantly towards the upliftment of livelihood of the poor and marginal farmers in these regions. in this co ... | 2017 | 28161212 |
| first report of immunohistochemical detection of peste des petit ruminants, parainfluenza 3 and respiratory syncytial viral antigens in lungs of nigerian goats. | this study determined the of involvement of ppr, pi3 and rs viruses in the pathology of caprine pneumonia across nigeria. 150 goats were selected randomly. pi3 and rsv monoclonal antibodies and ppr polyclonal antibody were used for the immunolocalisation of the antigens. histologically, 61 of the goats had broncho-interstitial pneumonia, 25 had interstitial pneumonia, 42 had bronchopneumonia, 12 had bronchiolitis and 10 were normal. ppr, pi3 and rs viral antigens were demonstrated in: intact and ... | 2017 | 28679075 |
| emetine inhibits replication of rna and dna viruses without generating drug-resistant virus variants. | at a noncytotoxic concentration, emetine was found to inhibit replication of dna viruses [buffalopoxvirus (bpxv) and bovine herpesvirus 1 (bhv-1)] as well as rna viruses [peste des petits ruminants virus (pprv) and newcastle disease virus (ndv)]. using the time-of-addition and virus step-specific assays, we showed that emetine treatment resulted in reduced synthesis of viral rna (pprv and ndv) and dna (bpxv and bhv-1) as well as inhibiting viral entry (ndv and bhv-1). in addition, emetine treatm ... | 2017 | 28624461 |
| development of a colloidal gold immunochromatographic strip based on hsp70 for the rapid detection of echinococcus granulosus in sheep. | echinococcus granulosus is the causative pathogen of cystic echinococcosis, a serious disease endangering human and animal health. in this study, an immunochromatographic strip was developed based on the recombinant protein heat shock protein 70 (hsp70) for the serological detection of e. granulosus. the protocol completes within 20min requiring no specialized equipment or chemical reagents, while specificity tests confirmed no cross-reactivity with positive serum of fasciola hepatica, haemonchu ... | 2017 | 28576342 |
| development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus. | goatpox virus (gtpv) and sheeppox virus (sppv), which belong to the capripoxvirus (capv), are economically important pathogens of small ruminants. therefore, a sensitive, specific and rapid diagnostic assay for detection of gtpv and sppv is necessary to accurately and promptly control these diseases. | 2017 | 28716095 |
| a g-protein-coupled chemokine receptor: a putative insertion site for a multi-pathogen recombinant capripoxvirus vaccine strategy. | capripoxviruses (capvs) have been shown to be ideal viral vectors for the development of recombinant multivalent vaccines to enable delivery of immunogenic genes from ruminant pathogens. so far, the viral thymidine kinase (tk) gene is the only gene used to generate recombinants. a putative non-essential gene encoding a g-protein-coupled chemokine receptor subfamily homologue (gpcr) was targeted as an additional insertion site. peste des petits ruminants (ppr) was chosen as a disease model. a new ... | 2017 | 28576653 |
| fatal peste des petits ruminants disease in chowsingha. | this communication reports fatal peste des petits ruminants (ppr) disease in chowsingha (tetracerus quadricornis), a member of the subfamily bovinae and family bovidae captured in a zoological park. the animals showed clinical signs of acute respiratory disease with frothy nasal discharge (1-2 days) and mortality of twenty animals (80%) within 48 hr. necropsy of dead chowsingha showed haemorrhagic patches in trachea and severe congestion of lungs and ocular mucosa. there was no characteristic le ... | 2017 | 28805043 |
| first report of peste des petits ruminants virus lineage ii in hydropotes inermis, china. | in this study, we investigated an outbreak of peste des petits ruminants (ppr) at a hydropotes inermis (water deer) farm in anhui province, china. these results demonstrated that ppr viruses (pprvs) can infect h. inermis and also revealed that virulent lineage ii pprvs exist in china, where they have been responsible for the deaths of wild animals. the government should pay close attention to the threat of pprv epidemiology in china. | 2017 | 28736887 |
| evolutionary dynamics of recent peste des petits ruminants virus epidemic in china during 2013-2014. | peste des petits ruminants virus (pprv) causes a highly contagious disease, peste des petits ruminants (ppr), in sheep and goats which has been considered as a serious threat to the local economy in africa and asia. however, the in-depth evolutionary dynamics of pprv during an epidemic is not well understood. we conducted phylogenetic analysis on genomic sequences of 25 pprv strains from china 2013-2014 outbreaks. all these strains clustered into a novel clade in lineage 4. an evolutionary rate ... | 2017 | 28734191 |
| vaccicost - a tool to estimate the resource requirements for implementing livestock vaccination campaigns. application to peste des petits ruminants (ppr) vaccination in senegal. | vaccination is one of the main tools currently available to control animal diseases. in eradication campaigns, vaccination plays a crucial role by reducing the number of susceptible hosts with the ultimate goal of interrupting disease transmission. nevertheless, mass vaccination campaigns may be very expensive and in some cases unprofitable. vaccicost is a tool designed to help decision-makers in the estimation of the resources required to implement mass livestock vaccination campaigns against r ... | 2017 | 28716194 |
| sero-prevalence and epidemiology of peste des petits ruminants in libya. | we conducted a cross-sectional study during 2013 to quantify the serological prevalence of peste des petits ruminants (ppr) infection and to investigate host factors associated with ppr infection in small ruminants in libya. a two-stage sampling design was carried out. a total number of 148 flocks owning at least 100 heads each were randomly selected. sixteen to forty-eight samples were collected from each selected flock. a total number of 3,508 serum samples from unvaccinated animals were colle ... | 2017 | 28703449 |
| screening of peste des petits ruminants virus in a population of district khairpur, pakistan. | goats are the pakistan's fastest growing ruminants, and pakistan is the third largest goat producer in the world after india and china. goat meat preference is the main reason for its increased demand. in the country, there are 25 goat breeds and two wild relatives such as mark and goats. at present, pakistan has 53.8 million goats, according to the 2006 gop report, and their population growth rate was more than 3% per year (37, 23, 22, and 18% of the goat population in punjab, sindh, balochista ... | 2017 | 28973722 |
| letter to the editor concerning "first report of peste des petits ruminants virus lineage ii in hydropotes inermis, china" by zhou et al. (transbound emerg dis; 2017: https://doi.org/10.1111/tbed.12683). | 2018 | 29476605 | |
| neglected hosts of small ruminant morbillivirus. | eradication of small ruminant morbillivirus (pprv) is targeted for 2030. pprv lineage iv is found in much of asia and africa. we used pprv lineage iv strain kurdistan/2011 in transmission trials to investigate the role of pigs, wild boar, and small ruminants as pprv reservoirs. suids were a possible source of infection. | 2018 | 30457523 |
| evaluation of a recombinant major envelope protein (f1l) based indirect- elisa for sero-diagnosis of orf in sheep and goats. | orf or contagious ecthyma, is a highly contagious transboundary disease of sheep and goats. for sero-diagnosis of orf, recombinant antigen based assays are considered as alternatives to conventional approaches such as serum neutralization test (snt) and counter-immuno-electrophoresis (cie). a major envelope protein of orf virus (orfv), f1l, is highly immunogenic and is a candidate for use in these assays. in this study, the f1l gene of the orfv-59/05 strain encoding a recombinant mature f1l prot ... | 2018 | 30149033 |
| first detection and genetic characterization of peste des petits ruminants virus from dorcas gazelles "gazella dorcas" in the sudan, 2016-2017. | in may 2017, many free-ranging dorcas gazelles (gazella dorcas) with suspected signs of peste des petits ruminants (ppr) were reported in dinder national park, south-eastern sudan. peste des petits ruminants virus (pprv) antigen and nucleic acid were detected in specimens from these gazelles using an immunocapture elisa and a reverse transcription polymerase chain reaction (rt-pcr) assays. pprv was also detected in four healthy semi-captive dorcas gazelles from two areas of khartoum state. phylo ... | 2019 | 31309291 |
| spatio-temporal analysis of peste des petits ruminants outbreaks in pr china (2013-2018): updates based on the newest data. | peste des petits ruminants (ppr) is a highly contagious disease of small ruminants. the purpose of this study was to investigate the epidemic characteristics of ppr outbreaks in the people's republic of china (pr china) from 2013 to 2018. a total of 41,876 ppr outbreaks were recorded in small ruminant populations in pr china during that period. data from february to june 2018 were used to study new developments in the ppr epidemic in pr china. spatio-temporal clusters and temporal distribution p ... | 2019 | 31207143 |
| pathological and molecular characterisation of peste des petits ruminants in nubian ibex (capra nubiana) in israel. | peste des petits ruminants (ppr) is a devastating disease that generally affects sheep and goats, mostly in asia, the middle east and africa. the disease has been declared a target for global eradication. despite its high prevalence in domestic flocks and its high seroprevalence among wildlife, it is rarely reported as a fulminant disease in wild ruminant species (with the exception of central asia). in this report, we describe a severe ppr outbreak in a zoo herd of nubian ibex (capra nubiana), ... | 2019 | 31089959 |
| experimental infection of dromedary camels with virulent virus of peste des petits ruminants. | peste des petits ruminants virus (pprv) causes a severe contagious disease of sheep and goats and has spread extensively in last years through asia and africa. pprv, known to infect exclusively small ruminants, has been recently reported in camels in iran and sudan. reported clinical symptoms are similar to those observed in small ruminants, fatality rate still unknown. however most of the authors reported seropositive camels without clinical signs. camel sensitivity to pprv is still controversi ... | 2019 | 31383302 |
| peste des petits ruminants (ppr) in dromedary camels and small ruminants in mandera and wajir counties of kenya. | a study was conducted to determine the presence of peste des petits ruminants (ppr) in camel population kept together with small ruminants in isiolo, mandera, marsabit, and wajir counties of kenya. this was done in the wake of a disease with unknown etiology "camel sudden death syndrome" camels in the horn of africa. thirty-eight (38) samples, 12, 8, 15, and 3 samples, were collected from mandera, wajir, isiolo, and marsabit, respectively, from 25 camels, 7 goats, and 4 sheep. one camel in mande ... | 2019 | 30956658 |
| productive replication of peste des petits ruminants virus nigeria 75/1 vaccine strain in vero cells correlates with inefficiency of maturation of the viral fusion protein. | peste des petits ruminants virus (pprv), a member of the genus morbillivirus, in the family paramyxoviridae expresses two membrane glycoproteins, the fusion (f) and haemagglutinin (h) glycoproteins which mediate virus-to-cell fusion and cell-to-cell fusion leading to the induction of syncytia in pprv infected cells. in the context of the characterization of the virulent lineage iv strain pprv kurdistan 2011, isolated from wild goats from the kurdistan region in iraq, we observed that both pprv k ... | 2019 | 31129173 |
| epidemic and evolutionary characteristics of peste des petits ruminants virus infecting procapra przewalskii in western china. | due to the migration or transboundary spread of domestic and wild animals, peste des petits ruminants virus posed a high potential threat to them. in this study, we initially detected that a class of animal named procapra przewalskii was infected with peste des petits ruminants virus (pprv chinags2018) in gansu province. according to phylogenetic relationships analysis, we found that chinags2018 comprised of 15,954 nucleotides and was classified into iv genotypes. in addition, indirect immunoflu ... | 2019 | 31415822 |
| molecular characterization of peste des petits ruminants viruses in the marmara region of turkey. | recent outbreaks of peste des petits ruminants (ppr) in the marmara region of turkey including the european part of thrace is important due to its proximity to europe (greece and bulgaria) and the potential threat of spread of ppr into mainland europe. in order to investigate the circulation of pprv in the region suspect clinical and necropsy samples were collected from domestic sheep (n = 211) in the marmara region of turkey between 2011 and 2012. ppr virus (pprv) genome was detected in 10.4% ( ... | 2019 | 30525310 |
| evidence of peste des petits ruminants' virus in dromedary camels in the kingdom of saudi arabia between 2014 and 2016. | infection with the peste des petits ruminants virus (pprv) is a highly devastating viral infection of small ruminants. dromedary camels live in close proximity of small ruminants in arabian peninsula (ap) and many other regions in the world. little is known about the reasons behind continuous pprv emergence in saudi arabia (ksa). our objective was to test some dromedary camel population across the kingdom for the presence of specific pprv antibodies. our results show detection of specific pprv a ... | 2019 | 32089813 |
| camelids and cattle are dead-end hosts for peste-des-petits-ruminants virus. | peste-des-petits-ruminants virus (pprv) causes a severe respiratory disease in small ruminants. the possible impact of different atypical host species in the spread and planed worldwide eradication of pprv remains to be clarified. recent transmission trials with the virulent pprv lineage iv (liv)-strain kurdistan/2011 revealed that pigs and wild boar are possible sources of pprv-infection. we therefore investigated the role of cattle, llamas, alpacas, and dromedary camels in transmission trials ... | 2019 | 31817946 |
| outbreak of peste des petits ruminants among critically endangered mongolian saiga and other wild ungulates, mongolia, 2016-2017. | the 2016-2017 introduction of peste des petits ruminants virus (pprv) into livestock in mongolia was followed by mass mortality of the critically endangered mongolian saiga antelope and other rare wild ungulates. to assess the nature and population effects of this outbreak among wild ungulates, we collected clinical, histopathologic, epidemiologic, and ecological evidence. molecular characterization confirmed that the causative agent was pprv lineage iv. the spatiotemporal patterns of cases amon ... | 2020 | 31855146 |
| a review of the current status of peste des petits ruminants epidemiology in small ruminants in tanzania. | peste des petits ruminants (ppr) is a highly contagious viral disease of sheep and goats with high mortality. the disease is of considerable economic importance in countries such as tanzania, where small ruminant products are important for sustainable livelihoods. this review assesses current knowledge regarding the epidemiology of pprv in tanzania, highlighting the challenges with respect to control and suggesting possible interventions. thirty-three articles were identified after literature se ... | 2020 | 33324702 |
| a novel recombinant newcastle disease virus vectored diva vaccine against peste des petits ruminants in goats. | peste des petits ruminants virus (pprv, species: small ruminant morbillivirus) is the causative agent of the eponymous notifiable disease, the peste des petits ruminants (ppr) in wild and domestic sheep and goats. mortality rates vary between 50% and 100%, causing significant losses of estimated 1.5 to 2 billion us dollars per year. live-attenuated pprv vaccine strains are used in the field for disease prevention, but the application of a more thermostable vaccine enabling differentiation betwee ... | 2020 | 32354145 |
| eradicating the scourge of peste des petits ruminants from the world. | peste des petits ruminants (ppr) is a highly contagious viral disease of both domestic (goats and sheep) and wild ruminants. caused by a morbillivirus, that belongs to the family paramyxoviridae. the disease is clinically and pathologically similar to rinderpest of cattle and human measles. ppr is one of the most economically devastating viral diseases of small ruminants. in april 2015, the food and agriculture organization of the united nations (fao) and the world organisation for animal health ... | 2020 | 32183412 |
| rnaseq reveals the contribution of interferon stimulated genes to the increased host defense and decreased ppr viral replication in cattle. | peste des petits ruminants virus (pprv) is known to replicate in a wide variety of ruminants causing very species-specific clinical symptoms. small ruminants (goats and sheep) are susceptible to disease while domesticated cattle and buffalo are dead-end hosts and do not display clinical symptoms. understanding the host factors that influence differential pathogenesis and disease susceptibility could help the development of better diagnostics and control measures. to study this, we generated tran ... | 2020 | 32325933 |
| peste des petits ruminants at the wildlife-livestock interface in the northern albertine rift and nile basin, east africa. | in the recent past, peste des petits ruminants (ppr) emerged in east africa causing outbreaks in small livestock across different countries, with evidences of spillover to wildlife. in order to understand better ppr at the wildlife-livestock interface, we investigated patterns of peste des petits ruminants virus (pprv) exposure, disease outbreaks, and viral sequences in the northern albertine rift. pprv antibodies indicated a widespread exposure in apparently healthy wildlife from south sudan (2 ... | 2020 | 32156067 |
| peste des petits ruminants in large ruminants, camels and unusual hosts. | since its first report in 1942, peste-des-petits-ruminants virus (pprv) has caused several epidemics in a wide range of susceptible hosts around the world. in the last 30 years, the evidence of natural and experimental infections and virus isolation were reported from novel but unusual hosts such as camel, cattle, buffalo, dogs, asiatic lion and pigs. in addition, pprv in a potential vector, biting midges (culicoides imicola), has been reported. either presented as clinical and/or subclinical in ... | 2020 | 31917649 |
| peste des petits ruminants virus transmission scaling and husbandry practices that contribute to increased transmission risk: an investigation among sheep, goats, and cattle in northern tanzania. | peste des petits ruminants virus (pprv) causes an infectious disease of high morbidity and mortality among sheep and goats which impacts millions of livestock keepers globally. pprv transmission risk varies by production system, but a deeper understanding of how transmission scales in these systems and which husbandry practices impact risk is needed. to investigate transmission scaling and husbandry practice-associated risk, this study combined 395 household questionnaires with over 7115 cross-s ... | 2020 | 32847058 |
| avidin-biotin recombinant nucleoprotein competitive elisa for the detection of peste des petits ruminants virus antibodies in sheep and goats. | the present study describes the development of a truncated recombinant peste des petits ruminants virus (pprv) nucleoprotein (rpprv-npn) and its polyclonal antibodies-based immuno-diagnostic assay, avidin-biotin (ab) recombinant nucleoprotein competitive elisa (abrc-elisa) for the detection of pprv antibodies in the sheep and goats. the pprv n-terminal immunogenic region (1-266 aa) of nucleoprotein (npn) coding sequence was amplified and cloned into the petite vector. the rpprv-npn with a molecu ... | 2021 | 34119607 |
| zinc finger antiviral protein (zap) inhibits small ruminant morbillivirus replication in vitro. | small ruminant morbillivirus (srmv) is a highly contagious and economically important viral disease of small domestic and wild ruminants. difficulty with its stable proliferation in ovis aries-derived cells has led to a relative lag in the study of its natural immunity and pathogenesis. here we report the antiviral properties of zap against srmv, a single-stranded negative-stranded rna virus of the genus morbillivirus. zap expression was significantly induced in sheep endometrial epithelial cell ... | 2021 | 34311269 |
| detection of peste des petits ruminants virus in pneumonic lungs from clinically apparently healthy camels slaughtered at tambul slaughterhouse, central sudan. | the study investigated the presence and prevalence of peste des petits ruminants (ppr) viral antigens among camels in tambul area, gezira state, central sudan, regardless of its sex, age and breed, and their possible contribution in the epidemiology of the disease in the sudan. hundred pneumonic lung tissues were aseptically collected from clinically apparently healthy camels showed no signs of illness at ante-mortem examination, from tambul slaughterhouse, tambul area, gezira state, central sud ... | 2021 | 33645909 |
| peste des petits ruminants virus infection at the wildlife-livestock interface in the greater serengeti ecosystem, 2015-2019. | peste des petits ruminants (ppr) is a viral disease of goats and sheep that occurs in africa, the middle east and asia with a severe impact on livelihoods and livestock trade. many wild artiodactyls are susceptible to ppr virus (pprv) infection, and some outbreaks have threatened endangered wild populations. the role of wild species in pprv epidemiology is unclear, which is a knowledge gap for the global strategy for the control and eradication of ppr. these studies aimed to investigate pprv inf ... | 2021 | 34066336 |
| serological evidence of bovine viral diarrhea virus and peste des petits ruminants virus infection in alpacas (vicugna pacos) in shanxi province, northern china. | bovine viral diarrhea virus (bvdv) and peste des petits ruminants virus (pprv) are two important pathogens associated with a variety of disease syndromes that result in substantial financial losses in animal husbandry. this study was performed to assess the seroprevalence of antibodies to bvdv and pprv among alpacas raised in shanxi province of northern china. serum samples were obtained from 246 alpacas in taiyuan (n=182), xinzhou (n=31), and jinzhong cities (n=33) of shanxi province, and serol ... | 2021 | 33939008 |