Publications

brucellosis as a cause of severe colitis.	a 16-yr-old girl presented with osteomyelitis and massive rectal bleeding. colonoscopy revealed severe nonspecific colitis. multiple laboratory investigations failed to disclose the etiology of either the bone or colon infections. empiric treatment with corticosteroids and sulfasalazine resulted in only transient improvement. one month after discharge, her original symptoms recurred. blood and pus cultures at this time yielded brucella melitensis. after tetracycline treatment, the patient recove ...	1991	2058640
[brucella endocarditis of bicuspid aortic valve. surgical treatment with successful result].	the authors report a case of brucella melitensis endocarditis of a bicuspid aortic valve which caused rapid progression of the hemodynamic signs of aortic stenosis, and was associated with a para-aortic abscess and a pericardial effusion. surgery resulted in correction of the hemodynamic abnormalities and cured the infection: the results were sustained 10 months after operation. this case illustrates the precision of the doppler, echocardiographic diagnosis of the lesions, which was confirmed at ...	1991	2064520
[the clinico-epidemiological characteristics of brucellosis in young people].	the article describes an outbreak of brucellosis among the servicemen (age 19-32) which was caused by br. melitensis. 13% of servicemen in the military unit went through this disease. this outbreak was caused by unsatisfactorily treated mutton of sheep which were affected with brucellosis. the illness had acute septic course. the appearance of the focuses of disease in locomotor system took place only when the infectious process had a rather long period (septicometastatic course). the acute sept ...	1992	1292191
[a carboxylated latex agglutination test for the serological diagnosis of human and animal brucellosis].	a carboxylated latex agglutination test was developed for detecting antibodies in sera of human and animals with brucellosis. the latex was chemico-linked with b. melitensis 16m extracted with 5% nacl. comparing with physico-sorbent latex antigens in detecting antibodies in sera of human and animals with brucellosis, the chemico-linked latex is more stable, more specific and more sensitive. in addition, preparation of chemico-linked latex and some factors affecting the result of the test were al ...	1992	1300250
control of brucellosis in kuwait by vaccination of cattle, sheep and goats with brucella abortus strain 19 or brucella melitensis strain rev. 1.	in kuwait, approximately 12,000 dairy cows were vaccinated with a reduced dose of 3 x 10(9) brucella abortus strain 19 and approximately 350,000 sexually mature sheep and goats with a reduced dose of 10(7) b. melitensis strain rev. 1. using the criteria of prevaccinal and postvaccinal incidences of antibodies, abortions, and human cases of brucellosis, the programme was very successful. widespread vaccination of adult animals is the most effective method of controlling brucellosis among cattle, ...	1992	1306918
brucella melitensis splenic abscess: sonographic detection and follow-up.		1992	1316379
in vitro activity of azithromycin and tetracycline against 358 clinical isolates of brucella melitensis.	the in vitro activity of azithromycin against human pathogenic strains of brucella melitensis was tested at three centres and compared to that of tetracycline, the standard antibiotic currently used for the treatment of human brucellosis. mic determination was carried out. tested concentration ranges for both azithromycin and tetracycline were between 0.03 and 16 micrograms/ml. brucella melitensis biotype 1, strain m16 was employed as a control microorganism. a total of 358 brucella melitensis s ...	1992	1317801
correspondence analysis in medical research.	various applications of correspondence analysis to biomedical data are presented. the basic concepts of profile, mass and chi-squared distance are introduced in an initial simple example using data on the relationship between headache types and age. the main result of the correspondence analysis is a geometric map of this relationship showing how the relative frequencies of headache types change with age. a second example maps the association between personality types and various medical diagnos ...	1992	1341654
biochemical mis-identification of brucella melitensis and subsequent laboratory-acquired infections.	brucella species are mis-identified in the api 20ne system as moraxella phenylpyruvica (profile number 1200004). since some brucella spp. grow readily in routine blood culture medium and may be isolated from patients without clinically obvious brucellosis, the risk of laboratory-acquired brucellosis exists. we describe two such cases.	1992	1358958
protection against brucella melitensis or brucella abortus in mice with immunoglobulin g (igg), iga, and igm monoclonal antibodies specific for a common epitope shared by the brucella a and m smooth lipopolysaccharides.	mice passively immunized prior to a challenge infection with immunoglobulin g (igg) and igm monoclonal antibodies (mabs) specific for a common epitope of both a- and m-dominant strains had viable brucella abortus 544 or brucella melitensis h38 counts in the spleen reduced to the same extent as did mice passively immunized with mabs specific for either the a or the m epitope. the iga mab was not effective.	1992	1370276
o-chain expression in the rough brucella melitensis strain b115: induction of o-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy.	spleen cells from mice infected with the rough brucella melitensis strain b115 were fused with nso myeloma cells. hybridoma supernatants were screened in elisa with cell walls (cw), sonicated cell extracts (ce) and rough lipopolysaccharide (r-lps) of b. melitensis strain b115 and whole b. melitensis b115 cells. surprisingly, 22 monoclonal antibodies (mabs) reacting in elisa with both cw and ce but not with r-lps and bacterial cells were shown by immunoblot analysis and elisa to react with smooth ...	1992	1382111
purification, characterization, and seroactivity of a 20-kilodalton brucella protein antigen.	an internal protein was purified from cell extracts of brucella melitensis b115 by a combination of preparative isoelectric focusing and high-performance size exclusion chromatography. the protein has an apparent molecular mass of 230 kda as determined by size exclusion chromatography. the protein was resolved to a single band of 20 kda after sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the native protein had an isoelectric point of 4.9. the n-terminal sequence of the 20-kda protei ...	1992	1400966
[osteoarticular manifestations of brucellosis. apropos of 2 cases of brucellosis-induced sacroiliitis].		1992	1411027
[elaboration of a rapid method of determining the sensitivity of brucella to antibiotics].	optimal conditions were developed for determination of antibiotic sensitivity in brucella by using enzyme immunoassay directly in the primary cultures of the material tested. the brucella concentration in the material tested should be not lower than 1.10(6) microbial cells/ml and the time of culture incubation be 24 hours at 37 degrees c. the obligatory condition is to use a liquid medium, i.e. the albimi broth with 1% glucose. to inhibit the foreign microflora it is recommended to use polymyxin ...	1992	1417320
a rapid and sensitive method for the identification of brucella species with a monoclonal antibody.	a coagglutination test using monoclonal antibody bme10-5 with specificity for the m antigen of brucella melitensis 16m has been developed for the rapid identification of the smooth brucella species. all reference strains of several biovars of b. melitensis, b. abortus, b. suis and b. neotomae tested were positive in this assay. no significant differences in reaction intensity were observed in relation to the different distribution of the a and m antigens among the brucella serovars analysed. con ...	1992	1448627
antibody response to brucella outer membrane proteins in bovine brucellosis: immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies.	sera from brucella-infected bovines were analyzed by immunoblotting by using sonicated cell extracts of b. melitensis or b. abortus and a competitive enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies against outer membrane proteins (omps) with molecular masses of 10, 16.5, 19, 25 to 27, 36 to 38, and 89 kda. antibody responses against omps were compared with antibody responses against smooth lipopolysaccharide. immunoblot analysis indicated that the antibody response in infect ...	1992	1452700
isolation of brucella melitensis from aborting ruminants in jordan.	thirty-four isolates (16.5 per cent) of brucella melitensis were cultured from 206 samples from aborted, still-born or weak full-term animals and vaginal swabs from aborted animals. twenty-six of 31 isolates from sheep were b. melitensis biovar 3. two isolates were biovar 1 and one of these was the vaccine strain rev 1. the three remaining isolates were from vaginal swabs and were not biotyped since b. melitensis biovar 3 had been isolated from the aborted fetuses. one of two isolates from cattl ...	1992	1452817
brucellosis with nephrotic syndrome, nephritis and iga nephropathy.	a patient with systemic brucellosis due to brucella melitensis had severe renal involvement. clinical features included hypertension, macroscopic haematuria, massive proteinuria of 10 g per 24 hours and azotaemia. following treatment with antibiotics, the azotaemia resolved and proteinuria decreased to less than 0.5 g per 24 hours, but microscopic haematuria and hypertension persisted. renal biopsy during recovery revealed iga nephropathy with minimal mesangial changes, suggesting a causal relat ...	1992	1461859
comparative in vitro activity of clarithromycin. spanish collaborative group.	the activity in vitro of clarithromycin, a new macrolide, was compared to that of various antibiotics in tests using 3,880 clinical isolates. clarithromycin was two times more active than erythromycin against staphylococcus aureus, enterococcus faecalis, enterococcus faecium, streptococci of groups c, g and f, brucella melitensis, legionella pneumophila and mycoplasma spp., 16 times more active against ureaplasma urealyticum and 2 to 4 times less active against campylobacter spp. in general, cla ...	1992	1468430
the ability of brucella melitensis to grow on loewenstein-jensen egg medium: presentation of a case with brucella meningitis.		1992	1468897
serological and bacteriological study of brucellosis in camels in central saudi arabia.	sera from 2,630 apparently normal adult camels (camelus dromedarius) raised in central saudi arabia (riyadh and al-kharj cities) were examined serologically by the rose bengal and standard united states of america brucella plate agglutination tests. the overall seroprevalence of brucellosis in the restricted populations of tested camels was 8%. the seroprevalence of brucellosis among camels raised in small numbers in the backyards of 24 houses in riyadh and those intensively raised on one large ...	1992	1472730
treatment of brucella melitensis infection in sheep and goats with oxytetracycline combined with streptomycin.	six treatment regimens using oxytetracycline (otc) combined with streptomycin (st) were evaluated for eliminating brucella melitensis from 480 naturally-infected sheep and goats. cessation of shedding brucella from udder secretions and absence of brucella in selected tissues at autopsy were considered criteria for successful treatment. four regimens were equally effective in eliminating brucella in the treated groups of sheep and goats regardless of the source of antibiotics used. these were reg ...	1992	1472731
[identification and characterisation of 200 strains of brucella canis under test from china].	200 cultures of brucella canis under test were received from some provinces in our laboratory. 167 (85.59%) of them were identified as brucella according to morphology, growth properties and serologies. and then, they were proved as brucella canis with r-serum agglutination and lysed test of phage r/c. some strains also were performed dna homologous determination and observation of electron microscopic morphology. the results found out that the genetic constitution and picture of them identified ...	1992	1481533
[the spectrum of pathogens in positive blood cultures--tyrol 1991].	in a recent study by the centers for disease control (cdc) it was noted that there had been a resurgence of gram-positive bacteremia together with an increase in fungemia. this reported trend is confirmed by data from the austrian tirol. in 1991 1,750 out of 13,679 specimens (12.8%) yielded bacterial or fungal growth, accounting for 1,248 cases of "bacteremia"; no decision was made about the clinical significance of the culture isolates. we consider laboratory reports of blood isolates to be fai ...	1992	1481545
unrecognised neurobrucellosis giving rise to brucella melitensis peritonitis via a ventriculoperitoneal shunt.		1992	1486897
[endocarditis caused by brucella melitensis on a mitral valve prosthesis presenting as an ischemic cerebrovascular accident].	a new case of endocarditis by brucella melitensis on a mitral valve prosthesis in a 15 year old patient, whose first manifestation was an ischemic cerebrovascular accident is reported. the patient presented with daily fever only two months later. medical treatment alone was not sufficient to avoid valvular failure and substitution of the prosthesis was required. the clinical manifestations and complications of this infrequent condition is discussed. treatment, which often requires the combinatio ...	1992	1489778
detection of brucella melitensis and brucella abortus by dna amplification.	suitable reaction conditions and oligonucleotide primers were sought for the detection of brucella melitensis and brucella abortus by the polymerase chain reaction. primers were chosen from within the coding sequence of a gene encoding a 31 kda b. abortus antigen. the test was shown to be sensitive, and specificity was demonstrated using dna derived from a panel of gram-negative pathogens. there was no detectable difference between b. melitensis and b. abortus in the sensitivity of the reaction ...	1992	1495123
[evolution of antibiotic sensitivity in brucella melitensis].		1992	1498179
therapy of experimental murine brucellosis with streptomycin, co-trimoxazole, ciprofloxacin, ofloxacin, pefloxacin, doxycycline, and rifampin.	mice infected with brucella melitensis were treated with streptomycin, co-trimoxazole, ciprofloxacin, doxycycline, and rifampin intraperitoneally and with ciprofloxacin, ofloxacin, pefloxacin, doxycycline, and rifampin orally for 14 to 21 days. doxycycline- and rifampin-treated animals (either route) demonstrated a cure rate significantly better than that of controls. longer therapy periods were associated with a significantly better outcome. therapy failure was observed in all mice treated with ...	1992	1510422
an elisa with brucella lipopolysaccharide antigen for the diagnosis of b. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival b. melitensis strain rev 1 vaccination.	an indirect enzyme-linked immunosorbent assay (elisa) with unpurified brucella melitensis smooth lipopolysaccharide (s-lps) as antigen was evaluated for the serological diagnosis of b. melitensis infection in sheep in comparison with the rose bengal (rb), complement fixation (cf), radial immunodiffusion (rid), microplate agglutination (ma) and rivanol agglutination (riv) tests. tests rb and cf detected as positive each of the 77 sera from b. melitensis-infected animals tested, the rid (74), ma ( ...	1992	1557898
preliminary studies on the use of carbon substrate utilization patterns for identification of brucella species.	a commercial system (biolog, hayward, ca) that uses reduction of an indicator dye to determine the oxidation of 95 different carbon substrates contained in a defined minimal medium was tested with 35 isolates of brucella spp. to determine if the system could be used in place of respirometric methods to identify species. of 95 substrates contained in this system, three were oxidized by all the brucella strains tested, 48 were oxidized by none of the strains tested, and 44 were oxidized differenti ...	1992	1572134
brucellosis induced avascular necrosis of the femoral head in a 7 year old child.	the case is reported of a 7 year old bedouin girl who presented with a three week history of fever and limp. initial tests for rheumatoid factor, antinuclear antibodies, and brucella were negative. two weeks after admission brucella melitensis was cultured from bone marrow and synovial fluid samples. the patient improved after treatment with doxycillin and streptomycin, but radiographs of the right hip showed avascular necrosis of the right femoral head. a mild limp and limitation of motion in t ...	1992	1575594
efficacy of ciprofloxacin for treatment of brucella melitensis infections.	the effectiveness of treatment of human brucellosis caused by brucella melitensis with ciprofloxacin alone was investigated in a prospective nonrandomized study. subjects with central nervous system involvement, endocarditis, or severe renal dysfunction; children under 16 years of age; and pregnant women were excluded from the study. of 19 patients, 16 completed the study; 7 were diagnosed as having acute systemic brucellosis, and 9 had acute brucella arthritis-diskitis. a rapid response to cipr ...	1992	1590681
evaluation of polysaccharide, lipopolysaccharide, and beta-glucan antigens in gel immunodiffusion tests for brucellosis in cattle.	in venezuela, 1,012 cattle sera were screened for their ability to precipitate brucella melitensis 16m smooth-lipopolysaccharide (s-lps), b melitensis b115 polysaccharide b (poly b), b abortus 1119-3 o-polysaccharide (ps), or b abortus 1119-3 cyclic 1,2 linked beta-d-glucan (beta-glucan) in an agar-gel immunodiffusion assay. these sera were previously classified as being brucella abortus-infected, s-19-vaccinated, or negative after an assessment of historical records and results of 5 standard se ...	1992	1595966
treatment of human brucellosis with doxycycline plus rifampin or doxycycline plus streptomycin. a randomized, double-blind study.	to compare the effectiveness of doxycycline-rifampin (dr) combination therapy with that of the classic doxycycline-streptomycin (ds) combination in patients with brucellosis.	1992	1596044
conjunctival vaccination of pregnant ewes and goats with brucella melitensis rev 1 vaccine: safety and serological responses.	when brucella melitensis strain rev 1 vaccine (rev 1) is administered by the standard method (1-2 x 10(9) viable bacteria injected subcutaneously), it may induce long-lasting serological responses and/or cause abortion in pregnant animals. the conjunctival route considerably reduces these drawbacks. in the present experiment a 1 x 10(8) cfu dose for both ewes and goats conjunctivally vaccinated at mid-pregnancy was tested for innocuousness (outcome of pregnancy, contamination of unvaccinated con ...	1992	1610080
rapid diagnosis of brucella melitensis in blood: some operational characteristics of the bact/alert.	a clinical isolate of brucella melitensis was detected in a blood culture with the bact/alert after an incubation period of 2.8 days. dilution studies revealed an inverse linear relationship between the log of the initial concentration of the organism and the time to detection of a positive result. reproducibility studies demonstrated a mean detection time of 48 +/- 1 h in 8 of 10 replicates seeded with a stock containing 10(2) cfu/ml.	1992	1734057
monoclonal antibodies to brucella rough lipopolysaccharide: characterization and evaluation of their protective effect against b. abortus.	we characterized 4 monoclonal antibodies (mab) specific for rough lipopolysaccharide (r-lps) of brucella. mab were selected by enzyme-linked immunosorbent assay (elisa) on whole b. abortus 45/20 rough cells and r-lps from b. melitensis b115 rough cells. specificity was confirmed by immunoblot analysis using r-lps and smooth lps (s-lps) preparations. anti-r-lps revealed the low molecular mass r-lps molecules below 20.1 kda in the r-lps and s-lps preparations as well as the typical a and m pattern ...	1993	7514802
brucella antigens--old dogmas, new concepts.	a brief review is given on concepts regarding brucella antigens, principally the lipopolysaccharide (lps) and o-polysaccharide but also, to a minor extent, proteins and lipids. although these views were reported years ago in the literature and became widely accepted at the time, many have since been disproven with results that have led to exciting new avenues of research and application. where gaps, contradictions and anecdotal observations suggest that the current understanding of brucella is l ...	1993	7519357
characterization of o-polysaccharide specific monoclonal antibodies derived from mice infected with the rough brucella melitensis strain b115.	twenty-two monoclonal antibodies (mabs) specific for smooth lipopolysaccharide (s-lps) were generated by fusion of spleen cells from mice infected with the rough brucella melitensis strain b115 with the nso myeloma. according to reactivity in enzyme-linked immunosorbent assay (elisa) with o-polysaccharide (o-ps) and absence of reactivity with rough lipopolysaccharide (r-lps), it was postulated that these mabs recognized epitopes present on the o-ps. most of the mabs reacted equally well in elisa ...	1993	7690392
[the effect of immunomodulators on the development of the infectious and vaccinal processes as well as on the efficacy of therapeutic preparations in brucellosis].		1993	8079591
brucella melitensis rev-1 vaccine as a cause of human brucellosis.		1993	8103891
unusual suppurative complications of brucellosis in children.	three cases with suppurative complications of brucella melitensis infection are presented, demonstrating localized involvement of the brain, bones and lungs, respectively, and the lesions were well defined on radiographic examination. the diagnosis was made on the basis of significant brucella titres and positive blood culture. awareness of such complications and performance of the appropriate serological and bacteriological studies will establish the diagnosis and will also differentiate this d ...	1993	8111184
brucella melitensis growth on loewenstein-jensen egg medium from a case of brucella meningitis.	bacteriologic investigation of a cerebrospinal fluid (csf) specimen for mycobacteria on lowenstein-jensen egg medium revealed the presence of brucella organisms. this coincided with a significant antibody titer against brucella spp. this is the first documented report of the ability of b. melitensis organisms to grow on loewenstein-jensen egg medium.	1993	8112041
identification and sequence analysis of is6501, an insertion sequence in brucella spp.: relationship between genomic structure and the number of is6501 copies.	an insertion sequence (is) element of brucella ovis, named is6501, was isolated and its complete nucleotide sequence determined. is6501 is 836 bp in length and occurs 20-35 times in the b. ovis genome and 5-15 times in other brucella species. analysis of the junctions at the sites of insertion revealed a small target site duplication of four bases and inverted repeats of 17 bp with one mismatch. is6501 presents significant similarity (53.4%) with is427 identified in agrobacterium tumefaciens, su ...	1993	8126444
brucellosis presenting as prolonged fever and hemophagocytosis.		1993	8213671
successful therapeutic regimens for treating brucella melitensis and brucella abortus infections in cows.	three therapeutic regimens were evaluated in 121 cows naturally infected with brucella melitensis or brucella abortus, using a combination of long-acting oxytetracycline (la-otc), streptomycin (st) and otc-intramammary infusion (imi). cessation of shedding of brucella in udder secretions and absence of brucella in selected tissues were considered criteria for successful treatment. regimen a (tested on 35 cows) consisted of la-otc 25 mg/kg administered intramuscularly (i.m.) every 3 days for 42 d ...	1993	8219341
anti-brucella activity of ro 23-9424, a dual-action antibacterial.	the in vitro activity of dual-acting antibacterial ro 23-9424 was determined by the agar dilution method against 126 clinical isolates of brucella melitensis. it was compared with fleroxacin, ciprofloxacin and five conventional drugs. mic50 and mic90 for ro 23-9424 were 1.0 and 4.0 mg/l, respectively, as compared with 0.25 and 0.5 mg/l for fleroxacin and ciprofloxacin. one strain which was resistant to other fluoroquinolones, with an mic of > 8.0 mg/l, did not show cross-resistance to ro 23-9424 ...	1993	8222864
brucella peritonitis.	we report the case of a patient with peritonitis caused by brucella melitensis who also had chronic liver disease. at first the patient was treated for bacterial peritonitis but when a lymphocytic ascites was aspirated antituberculosis chemotherapy was given. however, the serological tests for brucellosis were strongly positive and subsequently b. melitensis was isolated from ascitic fluid.	1993	8228298
prevalence of camel brucellosis in libya.	sera of 967 camels of both sexes were tested for antibodies to brucella using the rose bengal plate test, serum agglutination test and the complement fixation test. the prevalence of positive sera was 4.1 per cent. samples collected for cultural examination revealed 9 isolates. five isolates were from milk samples, 3 from aborted foetuses and one from a vaginal swab. all isolates were identified and biotyped as brucella melitensis biovar 1.	1993	8236486
evaluation of whole cell and subcellular vaccines against brucella ovis in rams.	five antigen preparations from brucella ovis strain reo 198 were incorporated with the pluronic polymer l-121 and muramyl dipeptide and tested as vaccines against b. ovis infection of rams. the antigenic preparations were: (1) a fraction enriched in outer membrane proteins and rough lipopolysaccharide (hot saline extract, hs); (2) the proteins from hs substantially free of lipopolysaccharide; (3) outer membrane blebs; (4) outer membrane-peptidoglycan complexes extracted with detergent; (5) kille ...	1993	8236802
quinolones in treatment of human brucellosis: comparative trial of ofloxacin-rifampin versus doxycycline-rifampin.	quinolones have been reported to be active against brucella species in vitro. in this prospective randomized study, the efficacy and safety of the combination of ofloxacin plus rifampin were compared with the efficacy and safety of doxycycline plus rifampin, both combinations administered for a 6-week period in treatment of brucellosis. sixty-one patients were enrolled in the study, and 49 had blood or bone marrow cultures positive for brucella melitensis. thirty patients received 200 mg of doxy ...	1993	8239591
simultaneous expression of smooth and rough phase properties related to lipopolysaccharide in a strain of brucella melitensis.	brucella strains exhibit either a rough (r) or a smooth (s) colonial phase identifiable by bacteriological methods. this depends on the biosynthesis and translocation to the surface in s but not in r strains, of the o-polysaccharide chain of the lipopolysaccharide (lps) molecule. b. melitensis biovar 1 strain ep exhibited simultaneously both s and r characteristics in relation to colonial morphology, agglutination by monospecific anti-m and anti-r sera, activity of bacteriophages lytic for rough ...	1993	8246253
brucellosis of the spine.	the spinal form of brucellosis is still a disabling disease in many countries outside north america and northern and central europe. fifteen consecutive cases of spinal brucellosis diagnosed and treated over a 20-year period were reviewed retrospectively. six patients were farmers, while 10 patients had a history of ingestion of unpasteurized milk or other dairy products. a high index of suspicion is necessary for the diagnosis, since there are no pathognomonic signs or symptoms. radiological as ...	1993	8264880
[brucellosis: case report and synopsis of 10 cases (1973-1992) in the chur canton hospital].	a 33-year-old patient from former yugoslavia presented with low back pain and fever. plain x-rays and myelography were normal, but a technetium-99m scan was suspect for vertebral osteomyelitis. serology and blood cultures were positive for brucella (melitensis). antibiotic therapy with tetracycline and rifampicine rapidly improved the signs and symptoms of the infection. a retrospective analysis of 11 cases of brucellosis treated in the kantonsspital, chur, over the years 1973 to 1992 revealed t ...	1993	8272813
therapy of experimental murine brucellosis with streptomycin alone and in combination with ciprofloxacin, doxycycline, and rifampin.	the in vivo efficacy of streptomycin (str), doxycycline (dox), rifampin (rif), ciprofloxacin (cip), and their combinations was evaluated for a brucella melitensis experimental infection in a mouse model. animals were infected with 2 x 10(4) to 4 x 10(4) cfu of b. melitensis intraperitoneally on day 0 and were randomized to receive, starting on day 7, str alone at 75, 150, or 300 mg/kg of body weight per day intraperitoneally or dox at 6 mg/kg/day orally, rif at 3 mg/kg/day orally, or cip at 200 ...	1993	8285615
sensorineural hearing loss in neurobrucellosis.	a case of neurobrucellosis presenting to the otologist with sensorineural hearing loss (snhl) as the predominant clinical feature is reported. the diagnostic features and treatment options are discussed and the need for prolonged combination treatment to prevent relapse and further deterioration of hearing stressed. snhl in neurobrucellosis has hitherto been reported principally in neurology literature as something of an incidental finding and so escapes the attention of otologists. it is hoped ...	1993	8288975
comparative analysis of brucella serotype a and m and yersinia enterocolitica o:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats.	hapten polysaccharides of brucella smooth m and a serotypes were prepared from brucella sp. and yersinia enterocolitica o:9 by previously described hydrolytic (o chain) or nonhydrolytic (native hapten [nh]) procedures. the purified polysaccharides differed only in the presence (o chain) or absence (nh) of lipopolysaccharide core sugars. the polysaccharides were compared by reverse radial immunodiffusion for the diagnosis of brucellosis in cattle (brucella abortus biotype 1 [a serotype] and bruce ...	1993	8308104
factors affecting detection of brucella melitensis by bactec nr730, a nonradiometric system for hemocultures.	the detection of brucella bacteremia by subculture does not always correlate with a positive signal in the bactec nr730 nonradiometric system (becton dickinson diagnostic instrument systems, sparks, md.). the effect of the inoculum size, ph, sodium polyanetholesulfonate, carbon sources (i-erythritol, sodium pyruvate, monosodium glutamate, d-glucose, and l-alanine), and urea in the release of co2 was evaluated by using the reference strain brucella melitensis 16m. in standard nr6 vials with or wi ...	1993	8308111
brucellar sacroiliitis: findings in 63 episodes and current relevance.	between 1974 and 1989 we prospectively observed 530 patients with brucellosis. the findings for 62 patients (42 males and 20 females; mean age, 34.7 years) with 63 episodes in which the sacroiliac joint was involved (the most frequent osteoarticular location [11.7%]) were analyzed. ten of the older patients (mean age, 55.3 years) had concomitant spondylitis. systemic symptoms were usually important, and characteristic pain and findings of sacroiliitis were observed in approximately 75% of cases. ...	1993	8329507
persistent coccobacillary sepsis in a preterm newborn.		1993	8345992
crystal structure to 2.45 a resolution of a monoclonal fab specific for the brucella a cell wall polysaccharide antigen.	the atomic structure of an antibody antigen-binding fragment (fab) at 2.45 a resolution shows that polysaccharide antigen conformation and fab structure dictated by combinatorial diversity and domain association are responsible for the fine specificity of the brucella-specific antibody, yst9.1. it discriminates the brucella abortus a antigen from the nearly identical brucella melitensis m antigen by forming a groove-type binding site, lined with tyrosine residues, that accommodates the rodlike a ...	1993	8358294
pancytopenia in children with brucellosis: clinical manifestations and bone marrow findings.	among 276 paediatric cases of brucellosis seen over a 7-year period, 16 patients (5.8%) with pancytopenia were identified. the most frequent presentations were fever, malaise, anorexia, weight loss, arthralgia, and hepatosplenomegaly. fourteen patients (87.5%) had positive blood and/or bone marrow cultures for brucella melitensis. bone marrow aspiration specimens showed hypercellularity in 14 patients and normocellularity in 2. histiocytes, eosinophils and plasma cells were increased in every ma ...	1993	8362601
[pathologic features of a case of systemic brucellosis due to brucella melitensis biovar 3 in a chamois (rupicapra rupicapra)].	a case of systemic brucellosis caused by brucella melitensis biovar 3 was identified in a chamois (rupicapra rupicapra) in the southern french alps. clinical signs included bilateral orchiepididymitis and keratoconjunctivitis, polyarthritis, and neurological signs; pathological findings confirmed an ulcerative keratitis, orchiepididymitis with calcified or necrotic foci and serofibrinous and hemorrhagic polyarthritis. the kidney cortex showed calcified nodules and infarctions. a small focus of c ...	1993	8364757
susceptibility of brucella melitensis to the new fluoroquinolone pd 131628: comparison with other drugs.	the antibacterial activity of pd 131628 was determined by the broth dilution method against 105 clinical isolates of brucella melitensis. fifty-two and ninety-nine percent of the isolates were inhibited by 0.06 and 0.12 microgram/ml of this quinolone, respectively. one strain which was resistant to 8 other fluoroquinolones, with a minimum inhibitory concentration (mic) of > 4.0 micrograms/ml, did not show cross-resistance and had an mic of 2.0 micrograms/ml. of the 9 fluoroquinolones tested, pd ...	1993	8384544
susceptibility of salmonella typhi and brucella melitensis to the new fluoroquinolone rufloxacin (mf 934).	the antimicrobial activity of the new fluoroquinolone rufloxacin (mf 934) was evaluated by a standardized agar dilution method against recent clinical isolates of salmonella typhi (67 strains) and brucella melitensis (108 isolates). the results were compared with 5 other commercially available or investigational fluoroquinolones. all the isolates of s. typhi were inhibited by 1.0 mg/l of rufloxacin as compared to 4-8 mg/l for b. melitensis. minimum inhibitory concentrations of rufloxacin against ...	1993	8396527
brucella group 3 outer membrane proteins contain a heat-modifiable protein.	brucella melitensis and b. ovis outer membrane blebs contained a protein displaying a temperature-dependent molecular mass upshift from 25 kda to 30 kda. a fraction of the protein tightly bound to lps did not show the molecular mass upshift which was also blocked by exposure of the protein to zwittergent 314. the b. melitensis heat-modifiable protein and escherichia coli ompa shared antigenic determinants. these data indicate that the brucella group 3 outer membrane proteins belonged to the ompa ...	1993	8405955
bone infection resembling phalangeal microgeodic syndrome in children. a case report.	phalangeal microgeodic syndrome of childhood is a rare condition and is the result of necrosis and repair within the phalanges. the cause is unknown. we present a case in which brucella melitensis was grown from one of the lesions.	1993	8409665
presence of two independent chromosomes in the brucella melitensis 16m genome.	mapping the restriction fragments of the brucella melitensis 16m genome with a new restriction endonuclease, paci, which cut the dna into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. pulsed-field gel electrophoresis of intact dna revealed two bands migrating the expected distances. these replicons were identified as two unique and independent chromosomes by the presence of rrna operons and genes for heat shock protei ...	1993	8423146
evaluation of vaccines and of antigen therapy in a mouse model for brucella ovis.	a mouse model was developed to study brucella ovis infection. the evolution of the number of b. ovis per spleen of mice inoculated intravenously, intraperitoneally or subcutaneously was found to be independent of the sex of the mice. the number of b. ovis increased in the spleen when increasing the challenge dose up to 1.7 x 10(7). at higher doses of challenge, the response remained constant. in this model it was observed that the inoculation of brucella melitensis rev 1 vaccine or subcellular b ...	1993	8427038
human brucellosis in dhofar, sultanate of oman.	sera were collected, mostly from school children, in six localities of the southern region of the sultanate of oman. macro and micro-agglutination tests were used to indicate positive brucella serology. four of the 525 sera tested had titres of at least 1:200, which were considered positive, and two had borderline values. the frequency of serologically positive sera in the six localities ranged between zero and 2%. no relevant difference was observed between titres using antigen of b. abortus or ...	1993	8429574
chronic brucella empyema.	brucellosis rates have decreased in developed countries in recent years, but the pathologic condition still occurs in developing countries and well-known endemic areas such as the mediterranean and middle eastern countries. a portuguese patient presented in switzerland with long-term pleural empyema, which was later diagnosed as brucella empyema through positive cultures of brucella melitensis. brucellosis should be considered in patients who have traveled to middle eastern countries or any othe ...	1993	8432167
[brucella melitensis: another possible cause of breast abscess++].		1993	8441291
[hematogenous pubic osteoarthritis].	pubic osteoarthritis is a little known pathological entity with a very controversial etiology. we present four cases of public osteoarthritis in which the infectious etiology was hematogeneous. the causal germs were: staphilococcus aureus, pseudomona aeruginosa, mycobacterium tuberculosis and brucella melitensis. in the first three cases, a surgical approach was used, allowing us to establish the etiological diagnosis of the process and to perform the local debridement. in the case of brucellar ...	1993	8448328
effect of age and duration of disease on the clinical manifestations of brucellosis. a study of 73 consecutive patients in israel.	we describe our experience with 73 patients diagnosed with brucellosis during the years 1979-91 at two jerusalem hospitals: hadassah mount scopus (37 patients from 1979-1984) and shaare zedek (36 patients from 1979-1991). the patients included 32 children less than 14 years old and 41 adults; 70 of the patients were non-jews. in all cases the pathogen was brucella melitensis. the high proportion of children and the equal sex distribution was quite different from the age and sex distribution of b ...	1993	8454438
spontaneous peritonitis due to brucella melitensis in a patient with cirrhosis.		1993	8462568
[bacteremia and multiple and recurrent skin ulcers due to brucella melitensis. a new modality of self-induced infection].	self-induced infections are a special form of factitious disease. they are characterized by the autoinoculation of contaminated substances or bacterial cultures which may lead to cutaneous infections, bacteremias, or arthritis by various germs. the conditions produced are difficult to diagnose and may lead to multiple operations, and sometimes mutilations or death of the patient. herewith, a patient who, over a period of 10 months, presented 4 episodes of bacteremia and cutaneous abscess in whic ...	1993	8464259
lymphocytes bearing the gamma delta t cell receptor in acute brucella melitensis infection.	a phenotypical analysis carried out by indirect immunofluorescence and two-color cytofluorometry showed that the number of lymphocytes bearing the gamma delta t cell receptor (tcr) heterodimer was dramatically increased in the blood of six children with brucella melitensis infection. most in vivo expanded gamma delta t cells reacted with a monoclonal antibody which identifies v delta 2 gene products and a significant proportion expressed cd25 and hla-dr activation antigens. in addition, whereas ...	1993	8477812
endocarditis caused by brucella melitensis.	to describe the management of an uncommon but serious infection in australia.	1993	8479384
brucellosis among hospital employees in saudi arabia.	to report experience with brucellosis among expatriate hospital employees in an area of the world where brucellosis is endemic.	1993	8496581
brucella mycotic aneurysm of ascending aorta complicating discrete subaortic stenosis.		1993	8498327
isolation of brucella melitensis from human milk.		1993	8505580
[immunobiological characteristics of brucella outer membrane proteins, genetic determinants of which are expressed in escherichia coli cells].	the influence of the brucella omp preparations obtained by the genetic engineering technique (18, 38 and 18 + 38 kd) on the formation of the specific protection and progress of infectious process in brucellosis in the in vivo experiments has been studied. the omps synthesized in escherichia coli cells gse579 and having mol mass 18 and 38 kd are common antigens for a number of brucella species. the 18 kd omp was found to protect 66.7% of experimental animals against brucellosis, while the protect ...	1993	8510678
hypothermia as a presenting sign of brucellosis.		1993	8513071
[the effect of the in-vitro interaction of antibacterial preparations on brucella melitensis].	the character of drug interaction in 18 various combinations of sisomicin, amikacin, gentamicin, cefotaxime, doxycycline, trimethoprim and nitroxaline was studied by the chess board method with respect to 25 strains of brucella melitensis. the synergistic effect with respect to the majority of the cultures was observed with the use of the combinations of gentamicin, amikacin or sisomicin with doxycycline. the combinations of the aminoglycosides with cefotaxime and sisomicin or gentamicin with ri ...	1993	8085903
[osteoarthritis caused by brucella melitensis: isolation of the microorganism from the synovial fluid].	brucellosis is an unusual disease in childhood but the organ manifestations are even more unusual. the authors describe an osteoarthritis caused by brucella melitensis, localized at the tarsal scaphoid in a three years old calabrian child.	1993	8197028
[acute brucellosis, pancytopenia and bone marrow hypoplasia].		1993	8289516
brucellosis: differential diagnosis of acute abdominal pain.	a 34-year-old turkish woman presented with septic fever, sweats, arthralgia, and abdominal pain. further examination revealed generalized lymphadenopathy, hepatosplenomegaly, pancytopenia, and multiple caseous granulomas. microbiological diagnosis revealed brucella melitensis type 3 as causative agent. this case report demonstrates that abdominal pain can be a symptom in brucellosis, and caseous granulomas may occur. in our mobile society brucella infection should be considered as possible diffe ...	1993	8291280
efficacy of brucella suis strain 2 vaccine against brucella ovis in rams.	the protective efficacy against brucella ovis of live vaccine brucella suis strain 2 (s2) and brucella melitensis strain rev 1 has been evaluated in rams. fourteen 4-month-old brucella-free aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. s2. sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) rev 1. thirteen rams were unvaccinated controls. eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. ...	1993	8296481
in vitro activity of eight fluoroquinolones against clinical isolates of brucella melitensis.	since fluoroquinolones have generated much interest because of their excellent in vitro and in vivo activity, their pharmacokinetic propertiesm oral dosing and intracellular penetration, we tested the susceptibility of 146 recent clinical isolated of brucella melitensis agaist eight 4-quinolone drugs and five conventional drugs. the drugs tested included ciprofloxacin, norfloxacin, temafloxacin, sparfloxacin, fleroxacin, pefloxacin, lomefloxacin, ci-960, tetracycline, gentamicin, streptomycin ri ...	1993	17587989
ci-990 (pd 131112): a new quinolone prodrug.	in vitro susceptibility of 1021 strains of recent clinical isolates was determined against the new fluoroquinolone ci-990 (pd 131112) and compared with ci-960, ciprofloxacin, fleroxacin, lomefloxacin, norfloxacin, perfloxacin, sparfloxacin, and temafloxacin. the minimum inhibitory concentrations (mic) of ci-990 in mg/l required for >90% isolates were 0.03-0.5 for members of enterobacteriaceae, 0.12-2.0 for pseudomonas aeruginosa, 0.03-1.0 for aeromonas hydrophilia, 1.0-2.0 for acinetobacter calc ...	1993	17588022
protective immunity to brucella ovis in balb/c mice following recovery from primary infection or immunization with subcellular vaccines.	experiments were performed with balb/c mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to brucella ovis and to compare infection immunity with immunity developed through vaccination with a hot saline extract (hs) of b. ovis. mice convalescing from a primary infection with b. ovis displayed a high level of resistance to reinfection, as evidenced by splenic bacterial counts decreased over 10,000-fold from control groups at 2 weeks ...	1994	8300219
southwestern internal medicine conference: brucellosis: don't let it get your goat!	brucellosis is a highly pleomorphic zoonotic infection caused by one of the following four species of gram-negative facultative intracellular coccobacilli: brucella melitensis, b. abortus, b. suis, or b. canis. the disease is a worldwide public health problem and a significant cause of economic losses in domestic live-stock. although largely eradicated in most industrialized countries, in the united states there has been an upsurge of b. melitensis cases associated with the ingestion of unpasteu ...	1994	8291510
antibody response to brucella melitensis outer membrane antigens in naturally infected and rev1 vaccinated sheep.	sera from brucella infected and b. melitensis rev1 vaccinated sheep were analysed by immunoblotting using the cell envelope fraction (cef) of b. melitensis b115. the cef of b. melitensis b115 was analysed using a bank of monoclonal antibodies. the fraction consisted mainly of s-lps like molecules, r-lps and outer membrane proteins (omps) of molecular masses of 10, 16.5, 19, 25-27, 31-34, 36-38, 73 and 89 kda. immunoblot analysis indicates that the antibody response in infected sheep was mainly d ...	1994	8203126
esophageal brucellosis: a new location of brucella infection.	the case of a patient with acute brucellosis presenting as lower dysphagia from a paracardial esophageal mass is described. brucella melitensis biotype 3 was isolated from cultured esophageal biopsy material. this is the first case of esophageal brucellosis described in the literature.	1994	8086774
high-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gd synthesized by baculovirus-infected insect cells.	two forms of herpes simplex virus glycoprotein gd were recombined into autographa californica nuclear polyhedrosis virus (baculovirus) and expressed in infected spodoptera frugiperda (sf9) cells. each protein was truncated at residue 306 of mature gd. one form, gd-1(306t), contains the coding sequence of patton strain herpes simplex virus type 1 gd; the other, gd-1(qaat), contains three mutations which eliminate all signals for addition of n-linked oligosaccharides. prior to recombination, each ...	1994	8289380
pleural fluid characteristics in pulmonary brucellosis.	although pulmonary symptoms accompany up to 16% of cases of infection with brucella melitensis, pleural effusion has rarely been reported. a 12 year old girl had brucellosis with pulmonary disease and a pleural effusion. the pleural fluid was clear and straw coloured with 2700 leucocytes/mm3 (93% lymphocytes), a protein level of 48 g/1, and a glucose concentration of 4.1 mmol/l. culture of the pleural fluid grew br melitensis.	1994	8153949
urban outbreak of a brucella melitensis infection in an argentine family: clinical and diagnostic aspects.	an outbreak of brucella melitensis in a family was studied. from the fourteen family members who ate unpasteurized goat cheese nine became ill. patients included four females and five males of 8 to 75 years old. in seven of the patients the diagnosis was confirmed by positive blood culture for b. melitensis biovar 1. all the patients were analyzed by standard tube agglutination (sta) and standard tube agglutination with 2-mercaptoethanol (sta-2me) tests at the time of diagnosis. in six of the pa ...	1994	8156050
brucellosis: an occupational hazard for medical laboratory personnel. report of five cases.	five cases of laboratory-acquired infection with brucella melitensis are reported. this pathogen is highly contagious when handled in the laboratory. clinicians should alert technologists when brucellosis is suspected so that specimens are handled under the most stringent safety measures. serological surveys and instructions of laboratory workers regarding the clinical diversity of the disease are helpful for early recognition of secondary cases. on the other hand, clinicians should consider bru ...	1994	8181839
identification of an immunoreactive brucella abortus htra stress response protein homolog.	an 11-kb fragment of brucella abortus genomic dna cloned into the bamhi site of puc9 expressed a 60-kda protein in escherichia coli dh5-alpha. antibodies reactive with this 60-kda protein were detected by western blot (immunoblot) analysis in sera from mice, cattle, and goats experimentally infected with b. abortus, in sera from mice experimentally infected with brucella melitensis, and in serum from a dog experimentally infected with brucella canis. similar results were seen with sera obtained ...	1994	8112833