Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| coronal leakage of obturated root canals after long-term storage using a polymicrobial marker. | this in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker on teeth root filled with lateral condensation of cold gutta-percha and one of two sealers. sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. the teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either apexit or tubliseal ewt sealer. the teeth were stored for 6 months in artificial saliva and tested for lea ... | 1997 | 9587271 |
| occurrence of prevotella nigrescens and prevotella intermedia in infections of endodontic origin. | the occurrence of prevotella intermedia and prevotella nigrescens in endodontic infections was studied using sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) of whole cell protein to distinguish between the species. previous studies have shown an association between black-pigmented bacteria (bpb) and endodontic infections and that prevotella intermedia (previously known as bacteroides intermedius) was the most commonly isolated bpb. recently, however, strains identified as p. ... | 1997 | 9587274 |
| etiology, diagnosis, and treatment considerations in peri-implantitis. | peri-implantitis is an inflammatory process affecting the tissues around an osseointegrated implant in function, resulting in loss of supporting bone. a review of the literature currently available leads to the conclusion that microorganisms play a major role in this disease. gram-negative anaerobic bacteria, particularly fusobacteria, spirochetes, and black-pigmenting organisms such as prevotella intermedia are often found in diseased sites. several treatment modalities are presently being eval ... | 1997 | 9655032 |
| distinction of prevotella intermedia and prevotella nigrescens from endodontic bacteremia through their fatty acid contents. | prevotella nigrescens has recently been recognized as a new species distinct from prevotella intermedia. the distinction is based largely on dna-dna hybridization, electrophoretic migration of malate and glutamate dehydrogenase, and peptidase and lipase activities of type strains. gas chromatography of cellular fatty acids can be a useful adjunct for characterization and identification of bacterial species. in the present study, cellular fatty acid profiles were determined for seven strains of p ... | 1997 | 16887563 |
| periodontal microflora of hiv infected patients with periodontitis. | the aim of this study was to determine the microbial profile of periodontal lesions in hiv seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. the subgingival microflora of 20 cdc ii, 20 cdc iii, 20 cdc iv/v and 20 systemically healthy patients with rapidly progressing periodontitis was examined. four sites with greatest probing depth in each patient were selected for microbiological sampling. the samples were cultured aerobicall ... | 1997 | 16887570 |
| ribotyping of bacteria from root canal and blood of patients receiving endodontic therapy. | dna restriction patterns and corresponding ribotypes of 26 bacterial isolates (propionibacterium acnes, peptostreptococcus prevotii, fusobacterium nucleatum, prevotella intermedia, prevotella nigrescens, actinomyces israelii, streptococcus intermedius, and streptococcus sanguis) recovered from patients with infected root canals and their peripheral blood, collected during and after endodontic therapy, were examined. eleven additional reference strains including type strains were also examined. p ... | 1997 | 16887597 |
| rapd fingerprinting for the distinction of prevotella intermedia sensu stricto from prevotella nigrescens. | a collection of 70 oral strains including reference strains and clinical isolates identified as prevotella intermedia sensu lato was constituted to cover a large clinical and geographical diversity. electrophoresis of the enzyme malate dehydrogenase allowed the identification of the 70 study strains as prevotella intermedia sensu stricto (n= 36), prevotella nigrescens (n= 31) and three unclassified strains. by using four primers, dna fingerprints were generated from 20 strains as random amplifie ... | 1997 | 16887601 |
| in vitro activity of linezolid and eperezolid, two novel oxazolidinone antimicrobial agents, against anaerobic bacteria. | linezolid (formerly u-100766) and eperezolid (formerly u-100592) are novel oxazolidinone antimicrobial agents that are active against multi-drug-resistant staphylococci, streptococci, enterococci, corynebacteria, and mycobacteria. preliminary studies also demonstrated that the compounds inhibited some test strains of anaerobic bacteria. therefore, we extended the in vitro evaluation of these agents to include a total of 54 different anaerobic species. minimal inhibitory concentration (mic) value ... | 1997 | 16887605 |
| clinical and microbiological effects of topical minocycline in the treatment of elderly patients with periodontitis. | to evaluate a 2% w/w minocycline gel (dentomycin) in the treatment of older adults with chronic periodontitis. | 1997 | 18069178 |
| possible lethal enhancement of toxins from putative periodontopathogens by nicotine: implications for periodontal disease. | to test the hypothesis that lethal synergy in the chick embryo model may occur between nicotine and bacterial products (cell-free extracellular toxins and cell lysates) of five putative periodontopathogens. | 1997 | 9155677 |
| the effects of smoking on periodontal structures: a literature review. | periodontal disease seems to be more prevalent in smokers than in nonsmokers. studies have reported both increases and decreases in gingival blood flow due to smoking. smoking does not increase the presence of the periodontopathogens actinobacillus actinomycetemcomitans, porphyromonas gingivalis and bacteroides intermedius. both the chemotaxis and the phagocytic capacity of the polymorphonuclear leukocytes (pmns) harvested from smokers are lower than with those harvested from nonsmokers. further ... | 1997 | 9170751 |
| hen egg-white lysozyme inhibits biological activities of lipopolysaccharides from periodontopathic bacteria. | lysozyme has a bactericidal activity for some strains of gram-positive bacteria, by enzymatic cleavage of peptidoglycans that constitute the cell wall. hen egg-white lysozyme (hel) was tested in vitro for effects on biological activities of lipopolysaccharides from periodontopathic gram-negative bacteria. actinobacillus actinomycetemcomitans, prevotella intermedia and porphyromonas gingivalis. hel inhibited a wide range of activities of these lipopolysaccharides, including activation of limulus ... | 1997 | 9138195 |
| development of resistance to metronidazole and minocycline in vitro. | by local delivery of antibiotics to periodontal pockets, very high initial concentrations are often quickly succeeded by subinhibitory concentrations, which may facilitate development of bacterial resistance. the purpose of the present study was to investigate possible development of resistance in suspected periodontal pathogens after exposure to subinhibitory concentrations of metronidazole and minocycline. the minimal inhibitory concentration (mic) of 18 reference strains and 12 clinical isola ... | 1997 | 9144048 |
| clinical and microbiological effects of subgingival antimicrobial irrigation with citric acid as evaluated by an enzyme immunoassay and culture analysis. | the purpose of the present study was to compare an enzyme immunoassay with culture samples from untreated and non-surgically treated periodontal pockets and to assess the clinical and microbiological effects of citric acid irrigation as a supplement to scaling and root planing. the enzyme immunoassay used in this study is a chairside diagnostic tool aimed at identifying the presence of p. gingivalis, p. intermedia, and a. actinomycetemcomitans. six sites with pocket depths > or = 6 mm in each of ... | 1997 | 9150039 |
| rapid diagnosis of periodontal infections: findings in aids patients. | a small number of bacterial pathogens in the human oral cavity cause the different forms of periodontal disease. of the approximately two hundred different oral bacterial species, about a dozen have been associated with these diseases including localized juvenile periodontitis, rapidly progressing periodontitis, and adult periodontitis. these species include actinobacillus actinomycetemcomitans, bacteroides forsythus, campylobacter rectus, porphyromonas gingivalis, and prevotella intermedia. sev ... | 1997 | 9037612 |
| short-chain carboxylic acid concentration in human gingival crevicular fluid. | short-chain carboxylic acids (e.g., lactic acid, propionic acid, butyric acid) are metabolic by-products of bacterial metabolism which can accumulate in the gingival crevice. it is of no small consequence, therefore, that 1- to 5-mm concentrations of these acids exhibit significant biological activity, including the ability to alter cell proliferation and gene expression in cells of importance to the periodontium. this communication reports on the in vivo concentrations of propionic and butyric ... | 1997 | 9042080 |
| microbial findings of deep peri-implant bone defects. | the objective of this study was to examine inflammatory tissue in deep peri-implant bone pockets (> 5 mm) for anaerobic bacteria colonization. the peri-implant inflammatory tissue of bone defects from 12 edentulous patients with 18 unsuccessful implants (imz type) was removed after surgical opening of the defects. after grinding the tissue with glass beads in nutrient solution, an aliquot of the suspension was plated and incubated on appropriate culture media. the quantitative and qualitative di ... | 1997 | 9048462 |
| the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia using an elisa in an adolescent population with early periodontitis. | the aim of the study was to compare the occurrence and levels of a. actinomycetemcomitans, p. gingivalis, and p. intermedia in the subgingival plaque from sites with and without early periodontitis in adolescents using an elisa. 47, 15- to 16-year-old adolescents (39 indo-pakistani, 8 white caucasian) were examined for clinical attachment level, probing depth, supragingival plaque, subgingival calculus and bleeding on probing on the mesio-buccal and disto-buccal aspects of the 1st molars and the ... | 1997 | 9049799 |
| prevalence of porphyromonas and prevotella species in the dental plaque of dogs. | the prevalence and populations of porphyromonas and prevotella species were determined in three samples of dental plaque from each of 34 healthy dogs. porphyromonas gingivalis was present in 68 per cent of the dogs and 47 per cent of the plaque samples. the counts of p gingivalis increased with age (p < 0.1), the amount of plaque (p < 0.05) and the degree of gingivitis (p < 0.1). prevotella intermedia was present in 44 per cent of the dogs and 23 per cent of the plaque samples. the counts of p i ... | 1997 | 9050176 |
| bacterial adherence to guided tissue regeneration barrier membranes exposed to the oral environment. | microbial colonization of barrier materials used in guided tissue regeneration (gtr) is known to adversely affect treatment outcomes. the purpose of this study was to compare the rate at which 11 commonly-occurring oral bacteria species colonize three different barrier materials (collagen, expanded polytetrafluoroethylene, and polylactic acid). the study group consisted of 10 systemically healthy individuals with no history of periodontal disease and absence of antimicrobial therapy within the p ... | 1997 | 9058336 |
| aerobic and anaerobic microbiology of bacterial tracheitis in children. | establish the aerobic and anaerobic microbiology of bacterial tracheitis in children. | 1997 | 9061728 |
| longitudinal study of prognostic factors in established periodontitis patients. | numerous indicators for disease progression have been described in the last decade. the purpose of this study was to examine, longitudinally, a large battery of clinical, microbiological, and immunological indicators, to try to determine whether the presence of one or a combination of these parameters at baseline, would correlate positively with increased attachment and or bone loss (true prognostic factors). following initial screening, 79 patients with established periodontitis were monitored ... | 1997 | 9062856 |
| prevalence of suspected periodontal pathogens identified using elisa in adolescents of differing ethnic origins. | the aim of this study was to determine the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in a group of adolescents and investigate the association of these organisms with various clinical parameters. a total of 527, 11-13-year-old children, of whom 333 (63%) were white caucasian, 187 (35%) indo-pakistani and 7 (1%) afro-caribbean, participated in the study. subgingival plaque samples, collected from the mesio-buccal of both upper first per ... | 1997 | 9083896 |
| a clinical and microbiological evaluation of systemic and local metronidazole delivery in adult periodontitis patients. | the present study describes results on selected clinical and microbiological parameters obtained by treatment with local (elyzol) and systemic (flagyl) use of metronidazole alone and/or mechanical subgingival debridement in adult periodontitis. patients were randomly divided into local and systemic treatment groups each comprising 5 individuals in each of whom 4 sites (one site/ quadrant) with a probing depth of > or = 5 mm were selected and treated with separate treatment modalities. the overal ... | 1997 | 9083899 |
| periodontal bacteria colonizing oral mucous membranes in edentulous patients wearing dental implants. | the purpose of this study was to investigate the prevalence of the microbiota on the oral mucosal surfaces and in the peri-implant pocket in edentulous subjects with a past history of periodontitis. twenty edentulous subjects wearing dental implants for at least one year and with a history of periodontitis participated in this study. clinical parameters were assessed (plaque, redness, swelling, peri-implant probing depth [pipd], and bleeding on probing) and microbiological samples were taken (or ... | 1997 | 9100195 |
| alveolar bone loss in rats infected with a strain of prevotella intermedia and fusobacterium nucleatum isolated from a child with prepubertal periodontitis. | prevotella intermedia and fusobacterium nucleatum are associated with various forms of periodontal disease. the purpose of the present study was to infect the clinical isolates of these periodontopathic bacteria and to induce a significant loss of alveolar bone in specific pathogen-free (spf) rats in the absence of ligatures. p. intermedia ykd8 and f. nucleatum ykz5 were isolated from a prepubertal periodontitis patient, while p. gingivalis mwb13 was from a patient with juvenile periodontitis. a ... | 1997 | 9029446 |
| detection and comparison of specific hemin binding by porphyromonas gingivalis and prevotella intermedia. | a radioligand assay was designed to detect and compare specific hemin binding by the periodontal anaerobic black-pigmenting bacteria (bpb) porphyromonas gingivalis and prevotella intermedia. the assay included physiological concentrations of the hemin-binding protein rabbit serum albumin (rsa) to prevent self-aggregation and nonspecific interaction of hemin with cellular components. under these conditions, heme-starved p. intermedia cells (two strains) expressed a single binding site species (4, ... | 1997 | 9006012 |
| intra-familial transmission and distribution of prevotella intermedia and prevotella nigrescens. | the periodontal bacteria prevotella intermedia and prevotella nigrescens have been recently separated from each other. the purpose of this study was to investigate the distribution and routes of transmission of these bacteria among family members. seven patients with moderate to severe periodontitis were selected. these probands, their spouses and 14 of their children were investigated. the presence of pr. intermedia and pr. nigrescens was determined by culture techniques in pooled subgingival p ... | 1997 | 9210087 |
| uvulitis caused by anaerobic bacteria. | i present two children with bacteremic uvulitis due to anaerobic bacteria. | 1997 | 9220512 |
| immune response to fusobacterium nucleatum, prevotella intermedia and other anaerobes in children with acute tonsillitis. | the number of aerobic and anaerobic bacteria was determined in the saliva of 20 children with acute group a beta-haemolytic streptococcal (gabhs) pharyngo-tonsillitis, and 20 with acute non-gabhs tonsillitis. antibody titres to four gram-negative anaerobic bacilli that reside in the oropharynx (fusobacterium nucleatum, prevotella intermedia, porphyromonas gingivalis, and actinobacillus actinomycetemcomitans) were determined in these and 20 control patients. an average of 8.8 aerobic and anaerobi ... | 1997 | 9222046 |
| 5 alpha reductase activity in human gingiva and gingival fibroblasts in response to bacterial culture supernatants, using [14c]4-androstenedione as substrate. | 5 alpha-reduction of androgen substrates is increased in inflamed gingiva. it was therefore relevant to study the effect of bacterial culture supernatants derived from prevotella intermedius (p.i), porphyromonas gingivalis (p.g) and actinobacillus actinomycetemcomitans (a.a) on the metabolism of [14c]4-androstenedione to 5 alpha-dihydrotestosterone (dht) in gingival tissue and cultured fibroblasts. chronically inflamed human gingival tissue and cultured gingival fibroblasts from the same source ... | 1997 | 9222443 |
| occurrence of invading bacteria in radicular dentin of periodontally diseased teeth: microbiological findings. | bacterial invasion in roots of periodontally diseased teeth, which has been recently documented using cultural and microscopic techniques, may be important in the pathogenesis of periodontal disease. the purpose of this investigation was to determine the occurrence and the species of invading bacteria in radicular dentin of periodontally diseased teeth. samples were taken from the middle layer of radicular dentin of 26 periodontally diseased teeth. 14 healthy teeth were used as controls. dentin ... | 1997 | 9226388 |
| optimized oligonucleotides for the differentiation of prevotella intermedia and prevotella nigrescens. | the gram-negative, anaerobic bacterium prevotella intermedia plays an important role in the progression of periodontitis, whereas the etiological role of the closely related but phenotypically indistinguishable species prevotella nigrescens is controversial. to differentiate between these species properly, 16s rdna/rna directed, computer-optimized oligonucleotides were designed and tested with 26 p. intermedia, 26 p. nigrescens and a number of closely and more distantly related strains. the olig ... | 1997 | 9227136 |
| the relationship between gingivitis and the serum antibodies to the microbiota associated with periodontal disease in children with down's syndrome. | gingival inflammation in down's syndrome children (ds) develops earlier and is more rapid and extensive than in non-ds children. abnormalities in host response to the oral flora have been proposed as etiological factors of this gingival inflammation. however, the relationship between gingivitis and the host response to oral microorganisms in ds by age has not been determined. the objective of this study was to clarify this relationship. sera were obtained from 75 ds subjects (aged 2 to 18 years) ... | 1997 | 9249633 |
| beta-lactamase producing bacteria in adult periodontitis. | in 23 untreated adult periodontitis patients, the occurrence of beta-lactamase producing periodontal bacteria was determined. in addition to non-selective isolation media, selective isolation and growth of beta-lactamase positive subgingival bacterial species was carried out on blood agar plates supplemented with amoxicillin and plates with amoxicillin+clavulanic acid. porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, peptostreptococcus micros, fusobacterium ... | 1997 | 9266340 |
| effects of metronidazole in patients with "refractory" periodontitis associated with bacteroides forsythus. | the aim of the present study was to monitor the microbiological and clinical effects of renewed supra- and subgingival debridement in conjunction with systemic metronidazole therapy (500 mg tid for 7 days) in 27 "refractory" periodontitis patients, culture positive for bacteroides forsythus and negative for actinobacillus actinomycetemcomitans. clinical evaluation included assessment of plaque, bleeding upon probing, probing pocket depth and clinical attachment loss at the deepest, bleeding site ... | 1997 | 9266345 |
| antimicrobial compounds from ceanothus americanus against oral pathogens. | during the search for antimicrobial compounds from higher plant sources, a methanol extract of ceanothus americanus demonstrated antimicrobial activity against selected oral pathogens. through further bioassay-guided fractionation and purification, three triterpenes (ceanothic acid, 27-hydroxy ceanothic acid and ceanothetric acid) and two flavonoids (maesopsin and maesopsin-6-o-glucoside) were identified. among these, ceanothetric acid and maesopsin-6-o-glucoside were new compounds. ceanothic ac ... | 1997 | 9276981 |
| increased susceptibility of rag-2 scid mice to dissemination of endodontic infections. | specific immunity has been implicated in the pathogenesis of periapical lesions, although the extent to which these mechanisms are actually involved in either protection or destruction of the pulp-periapex complex is yet to be established. to investigate this question we compared periapical-lesion pathogenesis in rag-2 severe combined immunodeficient (scid) mice with immunocompetent control mice following surgical pulp exposure. in order to equalize the bacterial challenge, an infection protocol ... | 1997 | 9284152 |
| role of porphyromonas gingivalis, prevotella intermedia, and prevotella nigrescens in extraoral and some odontogenic infections. | porphyromonas gingivalis, prevotella intermedia, and prevotella nigrescens were isolated from 138 subjects with various infections (intraabdominal, skin and soft-tissue, head and neck, pleuropulmonary, and odontogenic infections and bacteremia). the phenotypic identification of 173 isolates was completed by molecular methods. arbitrarily primed polymerase chain reaction (ap pcr) analysis was used to determine the genetic similarity of intraindividual p. intermedia/p. nigrescens group isolates re ... | 1997 | 9310676 |
| beta-lactamase production by oral pigmented prevotella species isolated from young children. | the frequency of beta-lactamase production by oral pigmented prevotella species isolated from 23 healthy young children and the minimal inhibitory concentrations (mics) for 186 available beta-lactamase-positive isolates were examined by using the chromogenic cephalosporin disk test (ab biodisk, solna, sweden) and the etest (ab biodisk) and/or the agar dilution method of the national committee for clinical laboratory standards (villanova, pa, usa), respectively. beta-lactamase-positive prevotella ... | 1997 | 9310704 |
| antibiotic resistance in an oral isolate of prevotella intermedia. | 1997 | 9310707 | |
| assay of menaquinones in plasma utilizing dual-electrode electrochemical detection. | 1997 | 9330307 | |
| cross-infection from periodontitis sites to failing implant sites in the same mouth. | twenty-five tooth and implant sites in nine patients were investigated for the presence of putative periodontopathic organisms, using specific dna probes for actinobacillus actinomycetemcomitans, porphromonas gingivalis, prevotella intermedia, eikenella corrodens, fusobacterium nucleatum, treponema denticola, and campylobacter recta. five of nine patients showed a likelihood of transmission from tooth to implant sites. these patients also showed a high number of putative periodontopathic organis ... | 1997 | 9337029 |
| synthetic histatin analogues with broad-spectrum antimicrobial activity. | histatins are salivary histidine-rich cationic peptides, ranging from 7 to 38 amino acid residues in length, that exert a potent killing effect in vitro on candida albicans. starting from the c-terminal fungicidal domain of histatin 5 (residues 11-24, called dh-5) a number of substitution analogues were chemically synthesized to study the effect of amphipathicity of the peptide in helix conformation on candidacidal activity. single substitutions in dh-5 at several positions did not have any effe ... | 1997 | 9337848 |
| fermentation product analysis in the identification of black-pigmented bacteria from periodontitis. | a quantitative procedure is described for the analysis of fermentation products of eight representative black-pigmented gram-negative anaerobic bacterial strains (porphyromonas gingivalis 381, porphyromonas gingivalis atcc 33277, prevotella intermedia atcc 25611, prevotella nigrescens atcc 33563, prevotella melaninogenica atcc 25845, prevotella denticola atcc 33185, and prevotella loescheii atcc 15930) from oral sites in humans, using gas-liquid chromatography. this procedure for the identificat ... | 1997 | 9345789 |
| characterization of the lactoferrin-dependent inhibition of the adhesion of actinobacillus actinomycetemcomitans, prevotella intermedia and prevotella nigrescens to fibroblasts and to a reconstituted basement membrane. | lactoferrin was previously shown to inhibit the adhesion of a. actinomycetemcomitans, p. intermedia and p. nigrescens to human cells. lactoferrin was also shown to competitively inhibit the binding of these bacteria to the basement membrane protein laminin. the present study aimed to determine the type of interactions inhibited by lactoferrin. lactoferrin binds to fibroblast monolayers and matrigel, a reconstituted basement membrane, through ionic interactions. the adhesion of a. actinomycetemco ... | 1997 | 9350211 |
| a novel component different from endotoxin extracted from prevotella intermedia atcc 25611 activates lymphoid cells from c3h/hej mice and gingival fibroblasts from humans. | a novel immunobiologically active fraction was prepared from a phenol-water extract of prevotella intermedia atcc 25611 by sephadex g-100 column chromatography. the fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. the fraction showed high-molecular-weight bands (10,000 to 12,000) on deoxycholate polyacrylamide gel electrophoresis (doc-page) and was scarcely active in a limulus test. we designated the fraction prevotella glycoprotein (pgp). the pgp fraction show ... | 1997 | 9353030 |
| anti-bacterial action of onion (allium cepa l.) extracts against oral pathogenic bacteria. | in this study, the focus was on the antibacterial activity of onions. this study researched the activities of onion extracts on streptococcus mutans and streptococcus sobrinus, the main causal bacteria for dental caries, and porphyromonas gingivalis and prevotella intermedia, considered to be the main causal bacteria of adult periodontitis. the results showed that the onion extracts possess an effect on all test bacterial strains (s.mutans jc-2, s. sobrinus omz176, p. gingivalis atcc 33277 and p ... | 1997 | 9354029 |
| putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis. | this study assessed the associations between putative periodontal pathogens and early-onset periodontitis (eop) in a population of 248 subjects, 13 to 19 years of age at baseline, derived from a representative sample of u.s. young adults. the subjects were selected based on the presence or absence of attachment loss at baseline. the attachment level was assessed clinically at baseline and at a 6-year follow-up examination, and the presence of 7 bacterial species was assessed at follow-up using d ... | 1997 | 9358364 |
| distribution and mobility of the tetracycline resistance determinant tetq. | we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ... | 1997 | 9372425 |
| experimental gingivitis in periodontitis-susceptible subjects. | the purpose of this study was to evaluate clinical, microbiological, and gingival crevicular fluid (gcf) profiles in periodontitis-resistant and periodontitis-susceptible subjects during 4 weeks of experimental gingivitis. experimental groups of similar ages were defined as gingivitis controls (gc; n = 10) and history of rapidly progressive periodontitis (rpp; n = 10), respectively. prior to baseline, all subjects achieved good plaque control (plaque index (p1i) approximately 0) and gingival hea ... | 1997 | 9378832 |
| a 5-year study of attachment loss and tooth loss in community-dwelling older adults. | tooth loss is a widely recognized endpoint measure for the effects of periodontal diseases and the impact of periodontal therapy. in fact, traditional clinical measures of periodontal status often are considered to be surrogate endpoints in that they are assumed to be related to tooth loss. however, the strength of the relationship between attachment loss and tooth loss in a representative population of untreated subjects has not been studied extensively. the purpose of this paper is to present ... | 1997 | 9379319 |
| "checkerboard" versus culture: a comparison between two methods for identification of subgingival microbiota. | the present study compared the "checkerboard" dna-dna hybridization methodology with culture techniques for the analysis of the composition of the subgingival microbiota. 70 subjects, presenting with a variety of periodontal conditions, contributed with a total of 283 subgingival plaque samples analyzed with respect to the following species: porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsy ... | 1997 | 9395099 |
| amoxicillin and clavulanic acid concentrations in gingival crevicular fluid. | the beta-lactams are bactericidal antibiotics, but some of them may be inactivated by bacterial beta-lactamases which destroy the beta-lactam ring. the inactivation of amoxicillin by beta-lactamases of gram negative anaerobic bacteria can be circumvented by the addition of clavulanic acid, a beta-lactamases inhibitor. thus, most of these bacteria are susceptible to this combination. the aim of this study was to investigate the concentrations of amoxicillin and clavulanic acid in gingival crevicu ... | 1997 | 9402501 |
| reduced serum igg reactivities with bacteria from dental plaque in hiv-infected persons with periodontitis. | serum samples were obtained from 44 hiv-seropositive (hiv+) and 37 hiv-seronegative (hiv-) persons that were grouped according to periodontal status. serum igg and iga reactivities towards streptococcus mutans, actinobacillus actinomycetemcomitans, porphyromonas gingivalis. prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum were measured by means of elisa. hiv+ persons with chronic marginal periodontitis showed significantly lower igg reactivities to the periodontal pathoge ... | 1997 | 9402504 |
| comparison of the subgingival microbiota of periodontally healthy and diseased adults in northern cameroon. | our study is the 1st report on subgingival microbiota in adult cameronians. the aim was to investigate, using the checkerboard dna-dna hybridization technique, the prevalence of 18 oral species in subgingival plaque samples obtained from sex- and age-matched cameronian adults with and without periodontal destruction. we also compared cultivation and the affirm dp test with the checkerboard technique in their capability to detect some selected species among the 18. 21 adult periodontitis patients ... | 1997 | 9402505 |
| clinical and microbiological effects of minocycline-loaded microcapsules in adult periodontitis. | clinical and microbiological effects of subgingival delivery of 10% minocycline-loaded (mc), bioabsorbable microcapsules were examined in 15 adult periodontitis patients. patients received oral hygiene instruction 2 weeks prior to the study. at baseline (day 0) all teeth received supragingival scaling (sc); 2 quadrants received no further treatment and 1 quadrant received subgingival scaling and root planning (srp). in the fourth quadrant, the tooth with the deepest probing sites (at least 1 sit ... | 1997 | 9407404 |
| [microflora of periodontal pockets in advanced periodontitis]. | the aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. from each subject 16-20 samples were taken using paper points. pooled sample after 60 s. mixing was serially diluted in reduced bhi. for total cell counts and for the isolation of black pigmented anaerobes brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without kanamycin-vancomycin mixture and bm agar plates were used. for isolation of a. actinomycetemcomitans ... | 1997 | 9411079 |
| comparative in-vitro and in-vivo activity of am-1155 against anaerobic bacteria. | the in-vitro activity of am-1155, a 6-fluoro-8-methoxy quinolone, was compared with those of temafloxacin, sparfloxacin, tosufloxacin, ciprofloxacin, ofloxacin and cefmetazole, a cephamycin, against a variety of anaerobic bacteria. although am-1155 demonstrated only modest activity against the bacteroides fragilis group and prevotella bivia (mic90s > or =3.13 mg/ml), 76% of the b. fragilis strains tested were inhibited at am-1155 concentrations of 0.78 mg/l. am-1155 was highly active against pre ... | 1997 | 9421310 |
| effect of triclosan on the subgingival microbiota of periodontitis-susceptible subjects. | the present study evaluated the long-term effect of (i) meticulous self-performed, supragingival plaque control and (ii) the use of a triclosan/copolymer containing dentifrice in adult subjects susceptible to destructive periodontitis. 40 individuals were recruited into the trial. 3-5 years prior to the baseline examination, they had all been treated by nonsurgical means- for advanced periodontal disease. during the subsequent maintenance phase, all subjects had at different time intervals exhib ... | 1997 | 9442424 |
| periodontitis patient and the spouse: periodontal bacteria before and after treatment. | suspected periodontal pathogens can be transmitted between spouses. the treatment response may be unsuccessful in periodontitis patients, if the spouse harbors these bacteria. the aim of the present 6-month follow-up study was to clarify whether the microbiological treatment outcome of periodontitis patients is related to the detection of suspected periodontal pathogens in the saliva of the spouse. 10 patients with advanced periodontitis and their spouses were included in the study. the patients ... | 1997 | 9442426 |
| subgingival temperature: relation to gingival crevicular fluid enzymes, cytokines, and subgingival plaque micro-organisms. | there have been no reports on the relationship of subgingival temperature to specific gingival crevicular fluid (gcf) components. therefore, the purpose of this cross-sectional study was to determine whether there was any relationship between subgingival temperature and gcf levels of neutrophil elastase (ne), myeloperoxidase (mpo), beta-glucuronidase (bg), interleukin-1 alpha (il-1), and interferon alpha (ifn). furthermore, another objective was to confirm an association of subgingival temperatu ... | 1997 | 9442427 |
| oral food consumption and subgingival microorganisms: subgingival microbiota of gastrostomy tube-fed children and healthy controls. | this study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube-fed children and 24 healthy controls. microbial identification was carried out using anaerobic culture and 16s rrna-based pcr identification methods. streptococcal and actinomyces species were recovered from 100% and 76% of all subjects and averaged 66% and 11% of total cultivable organisms, respectively. in decreasing order of prevalence, fusobacterium, ... | 1997 | 9444590 |
| prevalence of six periodontal pathogens detected by dna probe method in hiv vs non-hiv periodontitis. | the aim of the study was to examine the prevalence of selected periodontal pathogens associated with hiv and non-hiv related periodontal lesions. | 1997 | 9456680 |
| bacterial reduction in periodontal pockets through irradiation with a diode laser: a pilot study. | this study examines the application of a diode laser with a wavelength of 805 nm for periodontal treatment. while the use of the diode laser in this field has not been investigated so far, several authors have reported on the use of neodymium:yttrium-aluminum-garnet (nd:yag) laser for such applications. the aim of this study was to examine the immediate effect of the diode laser in reducing the bacterial concentration in periodontal pockets. important periodontal indices (pbi, cpitn) were assess ... | 1997 | 9467340 |
| extracts of prevotella intermedia and actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro. | in order to study the effects of sonicated extracts from prevotella intermedia, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and other oral-related bacteria, as well as escherichia coli on bone formation, clone mc3t3-e1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. the addition of the sonicated extracts from prevotella intermedia and actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose-dep ... | 1997 | 9467351 |
| acid tolerance response and survival by oral bacteria. | using 21 species of oral bacteria, representing six acidogenic genera, we undertook to determine whether the ph-limiting exponential growth is related to the ability of the organisms to generate an acid-tolerance response that results in enhanced survival at low ph. the lower ph limit of exponential growth varied by more than two units with that of neisseria a182 at ph 6.34; growth of lactobacillus casei rb1014 stopped at ph 3.81, with species of actinomyces, enterococcus, prevotella and strepto ... | 1997 | 9467379 |
| evidence for serum immunoglobulin g (igg) antibody responses in macaca fascicularis identified by monoclonal antibodies to human igg subclasses. | this investigation determined the capacity of murine monoclonal antibodies directed to human immunoglobulin g (igg) subclasses to identify molecules with conserved epitopes in the serum of the nonhuman primate, macaca fascicularis. we subsequently utilized this cross-reactivity to document the characteristics of igg subclass antibody responses in m. fascicularis to parenteral immunization with intact oral microorganisms, antigens from oral microorganisms, and finally a defined protein toxin, tet ... | 1997 | 9467387 |
| characteristics of systemic antibody responses of nonhuman primates to cell envelope and cell wall antigens from periodontal pathogens. | the immune response of the primate, macaca fascicularis, to cell envelope (cea) or cell wall (cwa) antigens of several periodontal pathogens was examined to develop a strategy to interfere with ligature-induced periodontitis. animals were parenterally immunized with cea of either porphyromonas gingivalis, prevotella intermedia or a combination of cea/cwa of campylobacter rectus, fusobacterium nucleatum and actinomyces viscosus. serum samples were taken every 2-4 weeks over a 4-month period, whic ... | 1997 | 9467388 |
| pathogenicity of prevotella intermedia and prevotella nigrescens isolates in a wound chamber model in rabbits. | the pathogenicity of 14 isolates identified as prevotella intermedia or prevotella nigrescens by serogrouping using monoclonal antibodies was compared in a tissue cage model in rabbits. seven strains from periodontal abscesses, 5 strains from deep periodontal pockets and 2 strains from gingivitis were tested in the animal model comprising 6 teflon tissue cages implanted on the back each of 34 rabbits. a total of 10(5)-10(8) cells of p. intermedia or p. nigrescens strains were inoculated alone or ... | 1997 | 9467400 |
| salivary iga responses to bacteria in dental plaque as related to periodontal and hiv infection status. | levels of total iga and specific iga reactive with streptococcus mutans, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum were measured by elisa in parotid saliva from hiv+ and hiv- persons with healthy gingiva (hg), chronic gingivitis, chronic marginal periodontitis (cmp), or necrotizing ulcerative periodontitis (nup). when the hiv+ group was compared with the hiv- group regardless of periodontal status, tot ... | 1997 | 9469606 |
| [bacteriological study of oral open abscesses]. | although studies of bacteriology of closed oral abscesses have been extensively done, there are few studies on microorganisms involving open oral abscesses. we examined bacteriologically three open abscesses with precaution against bacterial contamination with oral normal flora and saliva, when sampling. the specimens were subjected to aerobic and anaerobic cultures within 2 hours after sampling. all three cases were infected with 5 to 14 species of aerobic and anaerobic bacteria; streptococcus ... | 1997 | 9483884 |
| protein degradation by prevotella intermedia and actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum. | the proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown o ... | 1997 | 9178115 |
| contrasting effects of lipopolysaccharides (endotoxins) from oral black-pigmented bacteria and enterobacteriaceae on platelets, a major source of serotonin, and on histamine-forming enzyme in mice. | by measurement of serotonin levels, the translocation of platelets to various tissues was examined following intravenous injection of a lipopolysaccharide (lps) into c3h/hen mice. there was a rapid platelet accumulation (within 5 min and particularly in the lung), followed by a slower accumulation in the liver, which reached its plateau 3-5 h later. the severity of the anaphylactoid shock corresponded well with the magnitude of the rapid response. lpss from the oral black-pigmented bacteria, por ... | 1997 | 9180180 |
| periodontal findings in cohen syndrome with chronic neutropenia. | radiographic periodontal status and microbiological findings of periodontal pockets in subjects with cohen syndrome are presented in this report. this hereditary disorder causes mental retardation, and neutropenia is one feature of the syndrome. fifteen patients with cohen syndrome and 15 controls matched for age and sex and, as far as possible, according to the degree of mental retardation were examined. alveolar bone loss was evaluated from the panoramic radiographs. two subgingival samples we ... | 1997 | 9182743 |
| pcr-dna probe assays for identification and detection of prevotella intermedia sensu stricto and prevotella nigrescens. | the purpose of this study was to construct pcr-dna probe assays specific for prevotella intermedia sensu stricto and prevotella nigrescens based on the ability of randomly amplified polymorphic dna (rapd) fingerprinting to generate species-specific markers. the strategy included four steps: (i) construction of first-generation dna probes from a 850-bp rapd marker for p. intermedia sensu stricto and a 1,300-bp rapd marker for p. nigrescens, (ii) cloning and sequencing of each rapd marker, (iii) d ... | 1997 | 9196214 |
| modification of cystatin c activity by bacterial proteinases and neutrophil elastase in periodontitis. | to study the interaction between the human cysteine proteinase inhibitor, cystatin c, and proteinases of periodontitis associated bacteria. | 1997 | 9536278 |
| coronal leakage in teeth root-filled with gutta-percha and two different sealers after long-term storage. | this in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker in teeth root-filled with lateral condensation of cold gutta-percha and one of two sealers. sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. the teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either apexit or tubliseal ewt sealer. the teeth were stored for 6 months in artificial saliva and tested for lea ... | 1997 | 9550035 |
| dental plaque flora of the dog with reference to fastidious and anaerobic bacteria associated with bites. | animal bite wounds are amongst the most common types of traumatic injuries in humans. the organisms isolated from these wounds generally reflect the oral flora of the biting animal, and may be fastidious in nature and difficult to identify. this study was undertaken to determine the prevalence of eikenella corrodens, actinobacillus actinomycetemcomitans, porphyromonas and prevotella spp. in supragingival dental plaque collected from the right maxillary canine and carnassial teeth and the right m ... | 1997 | 9571899 |
| acid tolerance and acid-neutralizing activity of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum. | the tolerance to acid and the acid-neutralizing activity of three important periodontopathic bacteria, porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum were studied. p. gingivalis strains grew only at neutral ph and did not utilize glucose, whereas strains of p. intermedia and f. nucleatum could grow under acidic conditions and increased their growth by utilizing glucose. p. gingivalis tended to raise the culture ph during growth. p. intermedia and f. nucleatum raised ... | 1997 | 9573805 |
| identification of bacterial species on or in crevicular epithelial cells from healthy and periodontally diseased patients using dna-dna hybridization. | the purpose of this investigation was to identify bacterial species present on or in crevicular epithelial cells in healthy and diseased sites using dna probes. in order to achieve this aim, further improvements were made in the separation of unattached bacteria from those adherent to epithelial cells isolated from the human gingival crevice or periodontal pocket. then the dna probes were used to determine the prevalence of detectable dna from 15 microbial species on or in crevicular epithelial ... | 1998 | 9573819 |
| beta-lactamase-producing strains in the species prevotella intermedia and prevotella nigrescens. | a total of 96 strains were collected that included laboratory strains and clinical isolates classified prevotella intermedia sensu lato and the type strains of the species p. intermedia sensu stricto and prevotella nigrescens. susceptibility to amoxicillin and amoxicillin-clavulanic acid was determined by the etest. pcr-dna probe assays were used to speciate each strain as p. intermedia sensu stricto or p. nigrescens. by etest, 71 strains (74%) were susceptible to both amoxicillin and amoxicilli ... | 1998 | 9573820 |
| characterization of a novel n-acetylneuraminic acid-specific fusobacterium nucleatum pk1594 adhesin. | fusobacterium nucleatum has been identified as significantly associated with sites with active periodontal disease and, as a group, the oral fusobacteria coaggregate with members of all oral bacteria genera tested. monoclonal antibodies were prepared and used in conjunction with other potential inhibitors, such as simple sugars and amino acids, to characterize coaggregation interactions, of f. nucleatum pk1594. four unique monoclonal antibodies, 5h11, 14c7, 19f2 and 29c12, were obtained by their ... | 1998 | 9573822 |
| the binding and utilization of hemoglobin by prevotella intermedia. | prevotella intermedia, a putative periodontopathic microorganism, requires iron for growth. hemoglobin can be a major source of iron for bacterial growth in vivo since it is present in the crevicular fluid collected from periodontitis sites. experiments studying the growth of p. intermedia in iron-depleted todd-hewitt broth supplemented with human hemoglobin showed that the bacteria were able to utilize human hemoglobin as a source of iron. the uptake of iron from hemoglobin by p. intermedia app ... | 1998 | 9627957 |
| the use of metronidazole and amoxicillin in the treatment of advanced periodontal disease. a prospective, controlled clinical trial. | the present clinical trial was performed to study the effect of systemic administration of metronidazole and amoxicillin as an adjunct to mechanical therapy in patients with advanced periodontal disease. 16 individuals, 10 female and 6 male, aged 35-58 years, with advanced periodontal disease were recruited. a baseline examination included assessment of clinical, radiographical, microbiological and histopathological characteristics of periodontal disease. the 16 patients were randomly distribute ... | 1998 | 9650870 |
| the effect of herbal extracts in an experimental mouthrinse on established plaque and gingivitis. | the purpose of the present study was to establish in vitro the inhibiting effect of a herbal extract mixture on a selected number of micro-organisms and to test in vivo the effect of a mouthwash containing 6.3 mg/ml herbal extract mixture on plaque and gingivitis as compared to a minus active control mouthrinse. the herbal extract was a mixture of: juniperus communis (juniper), urtica dioca (nettle), achillaea millefolium (yarrow); 1:1:1. in the study, in-vitro, the effect of pure herbal extract ... | 1998 | 9650877 |
| on the discussion concerning microbiological culture and dna probes in the detection of periodontopathogenic bacteria. | 1998 | 9650883 | |
| purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium prevotella intermedia. | a novel alkaline phosphatase, designated pialp, has been purified and characterized from prevotella intermedia atcc 25611, an anaerobe implicated in progressive periodontal disease. the enzyme was a homodimer of apparently identical subunits of mr 54 kda. thiol-reducing agents completely inhibited the purified enzyme. the enzyme was highly stable even at 80 degrees c. it exhibited substantial activity against tyrosine-phosphate-containing raytide. the phosphatase activity was sensitive to orthov ... | 1998 | 9654126 |
| [gingival hyperplasia in pregnancy. ii. etiopathogenic factors and mechanisms]. | the principal aspects of the hyperplastic pathology which frequently appears during pregnancy with a particular regard to the etiopathogenetic hypotheses are presented. the etiopathogenesis of the so-called pregnancy granuloma is rather complex, because in addition to the common irritant factors like bacterial plaque and calculus it is necessary to recognize the influence that hormonal modifications in pregnancy have on the predisposition to the development of gingival lesions. the hormonal infl ... | 1998 | 9677819 |
| citrate promotes attachment of prevotella nigrescens (intermedia) atcc 25261 to hydroxyapatite. | large quantities of prevotella nigrescens (intermedia) atcc 25261 (p. nigrescens) cells adhere to hydroxyapatite (ha) treated with citrate, but do not adhere experimental pellicle prepared from human whole saliva. to determine the nature of the citrate responsible for promoting p. nigrescens cell adhesion, the duration and frequency of citrate treatment of ha and the inhibitory effect of other carboxylates were tested. the citrate rapidly adhered to ha beads in less than 15 min. with a lower con ... | 1998 | 9680763 |
| influence of hormonal contraceptives on microbial flora of gingival sulcus. | to determine a possible influence of two different hormonal contraceptives on bacterial microflora of gingival sulcus, subgingival plaque samples of 29 healthy women aged between 20 and 32 years were investigated bacteriologically before subjects took a contraceptive and 10 and 20 days after subjects started the medication. in 14 women, and oral contraceptive containing 0.02 mg ethinyl estradiol and 0.15 mg desogestrel (preparation a) was used, and 15 women took a contraceptive containing 0.03 m ... | 1998 | 9693397 |
| the effect of supragingival plaque control on the progression of advanced periodontal disease. | the aim of the present trial was to study the effect of meticulous supragingival plaque control on (i) the subgingival microbiota, and (ii) the rate of progression of attachment loss in subjects with advanced periodontal disease. an intra-individual group of sites exposed to non-surgical periodontal therapy served as controls. 12 patients with advanced periodontal disease were subjected to a baseline examination (bl) including assessments of oral hygiene status, gingival condition (bop), probing ... | 1998 | 9696252 |
| guided tissue regeneration for the treatment of intraosseous defects using a biabsorbable membrane. a controlled clinical study. | the aim of this controlled, clinical study was to evaluate guided tissue regeneration using a bioabsorbable membrane in periodontal intraosseous defects. forty patients, each contributing one defect > or =4 mm in depth participated. the control group (18 individuals) received conventional flap therapy, while the test group (22 individuals) was treated using the bioabsorbable membrane, guidor. clinical assessments were made by one examiner, blinded with respect to treatment group, at baseline, 6 ... | 1998 | 9696260 |
| characteristics of prevotella intermedia-specific cd4+ t cell clones from peripheral blood of a chronic adult periodontitis patient. | periodontitis is a chronic destructive inflammatory disease associated with periodontopathic bacteria. in addition, autoantigens such as collagen and heat shock proteins (hsp) have been suggested to play a role. established periodontal lesions are characterized by dense infiltrations of immune cells such as cytokine-producing cd4+ and cd8+ t cells. cd4+ t cells specific for prevotella intermedia can be isolated from lesional gingiva, suggesting an active role for cd4+ t cells in the response to ... | 1998 | 9697992 |
| effects of topical metronidazole and tetracycline in treatment of adult periodontitis. | the present study was performed to assess and compare the clinical healing and the microbiological findings following local application of metronidazole or tetracycline to augment subgingival scaling in previously untreated adult periodontitis sites. eighteen patients with moderate to severe adult periodontitis at single-rooted teeth were selected. in each patient, 3 interproximal sites having comparable root anatomy, probing depth > or =5 mm and bleeding on probing were randomly assigned to 1 o ... | 1998 | 9706861 |
| the utility of whole saliva to detect the oral presence of periodontopathic bacteria. | this study compared the presence of 6 periodontopathic bacteria in whole saliva and subgingival plaque of 202 subjects. the test bacteria were identified using a 16s rrna-based pcr detection method. each study subject contributed a whole saliva sample and a paper point sample pooled from the deepest periodontal pocket in each quadrant of the dentition. the kappa test revealed a fair agreement between the presence of porphyromonas gingivalis, prevotella intermedia, and treponema denticola in whol ... | 1998 | 9706862 |
| dual regulatory effects of interferon-alpha, -beta, and -gamma on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from prevotella intermedia, interleukin-1alpha, or tumor necrosis factor-alpha. | in a previous study, we demonstrated that the amount of interleukin (il)-8 mrna expressed by human gingival fibroblasts stimulated with lipopolysaccharide (lps) from prevotella intermedia atcc 25611 is increased by pre-treatment with beta or gamma interferon (ifn-beta or -gamma). in the present study, we identified the regulatory effects of these ifns on il-8 mrna expression and il-8 production by human gingival fibroblasts. priming with ifn-alpha (alpha), -beta, or -gamma upregulated the il-8 m ... | 1998 | 9719033 |
| subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes. | the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ... | 1998 | 9722266 |
| crevicular fluid level of beta-glucuronidase in relation to clinical periodontal parameters and putative periodontal pathogens in early-onset periodontitis. | analysis of beta-glucuronidase (betag) in the gingival crevicular fluid (gcf) provides an indication of neutrophil influx into the crevicular environment. the aim of this study was to test the hypotheses that: (1) betag is significantly elevated in individuals with early-onset periodontitis (eop) and that betag activity correlates with disease severity; and (2) betag level may reflect the local bacterial challenge in the gingival crevice. the study subjects consisted of a sub-sample of individua ... | 1998 | 9722267 |
| oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis. | the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ... | 1998 | 9722273 |