Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| molecular events leading to fusiform morphological transformation by partial src deletion mutant of rous sarcoma virus. | a mutant of the prague strain of rous sarcoma virus (rsv) has two deletions near the 5'-terminus of its src gene. the mutant (dl 5) induced fusiform morphological transformation in chick embryo fibroblasts (cef) in contrast to the round type morphological transformation induced by wild type (wt) rsv. the partially deleted src of dl5 was shown to code for a 52k protein which has tyrosine kinase activity as high as that of p60src. in dl 5-transformed cells, a 36k protein was heavily phosphorylated ... | 1983 | 6188271 |
| transcriptional control of the fibronectin gene in chick embryo fibroblasts transformed by rous sarcoma virus. | rous sarcoma virus transformed-chick embryo fibroblasts contain decreased amounts of the adhesive protein fibronectin that results from a lowering of fibronectin mrna levels (fagan, j.b., sobel, m.e., yamada, k.m., de crombrugghe, b., and pastan, i. (1981) j. biol. chem. 256, 520-525). in the present study, nuclei isolated from chick embryo fibroblasts (cef) and rous sarcoma virus transformed-chick embryo fibroblasts (rsv-cef) were used to measure relative rates of fibronectin rna synthesis. [32 ... | 1983 | 6189838 |
| transformation parameters and pp60src localization in cells infected with partial transformation mutants of rous sarcoma virus. | rous sarcoma virus (rsv)-induced transformation is mediated by the action of the viral src gene product pp60src. this transforming protein is found at several cytoplasmic locations, including the adhesion plaques of rsv-transformed cells. in these studies, we have focused on the adhesion plaque location of pp60src and determined whether any of the induced transformation parameters correlate with the presence of pp60src in the adhesion plaques. a series of partial transformation mutants of rsv th ... | 1983 | 6190075 |
| rous sarcoma virus-induced changes in the pattern of phosphorylation of non-histone nuclear proteins. | we here studied the protein kinase activity and in vitro phosphorylable sites of non-histone nuclear proteins, 0.4 m nacl extracts (mostly chromosomal proteins) from chick embryo fibroblasts (cef), infected or not with a schmidt ruppin strain subgroup a of rous sarcoma virus (rsv). the infection and transformation of chick fibroblasts by rsv induced an increase in kinase activity and endogenous phosphorylation of non-histone chromosomal (nhc) proteins. the stimulation, by a change of medium, of ... | 1983 | 6190663 |
| inhibition of rous sarcoma virus assembly by treatment with 2',5' adenosine nucleotides. | we have investigated the influence of 2',5' adenosine nucleotides on the replication and transformation of cells by rous sarcoma virus (rsv). treatment with the nucleotides ppp2',5'a4 and 2',5'a4 causes a striking reduction (50-fold) in the yield of infectious progeny virus, while ppp2',5a2 and 2',5'a3 had virtually no effect. the reduction in infectivity seen with 2',5'a4 nucleotides is paralleled by a smaller but significant (three- to four-fold) reduction in the amount of particles released a ... | 1983 | 6191438 |
| immunological study of a cellular 35k phosphorylated polypeptide detected in rous sarcoma virus transformed cells. | we have immunoprecipitated a phosphoprotein of 35k daltons (35k) common to rsv-transformed chick embryo fibroblasts (cef) and rodent cells. the phosphorylation of this antigen depends on the expression of the v-src gene and contains phosphotyrosine. the pre-existing 35k protein, of cef infected de novo, was further shown to become phosphorylated shortly after the appearance of active pp60v-src, and about 1 day before morphological transformation. the experiments with rsv-transformed rodent cells ... | 1983 | 6195999 |
| transformation of rat cerebral endothelial cells by rous sarcoma virus. | rat cerebral microvascular endothelial cells were infected with schmidt-ruppin rous sarcoma virus-strain d (sr-rsv-d), an avian retrovirus. a single focus of transformed cells was isolated and the resultant cell line designated rce-t1. the specificity for sr-rsv-d transformation was determined by virus rescue assay and demonstration of virus-specific antigens. rce-t1 cells are virogenic when fused with chicken embryo fibroblasts (cef) and do not produce infectious virus as demonstrated by the ab ... | 1983 | 6134735 |
| respiratory syncytial virus infection in syrian hamsters. i. development of humoral immunity. | adult syrian hamsters have been shown useful for the study of respiratory syncytial virus (rsv) infection. clinical manifestations coincided with the maximum replication of the virus in respiratory tract of infected animals as confirmed by immunofluorescence (if) test. the dynamics of secretory and serum antibody responses is described in infected and reinfected animals. | 1983 | 6140834 |
| respiratory syncytial virus infection in syrian hamsters. ii. regulation of the immune response. | the dynamics of immune cell responsiveness in the process of development of primary and secondary immune responses after infection of adult syrian hamsters with native respiratory syncytial virus (rsv) was studied. in primary immune response, the enhancement of functional responsiveness of lymphocytes to mitogens and the maximum level of their spontaneous proliferative activity were found to precede the optimal level of antibody synthesis. regulation of the dynamics of immunogenesis after reinfe ... | 1983 | 6140835 |
| a cellular protein phosphorylated by the avian sarcoma virus transforming gene product is associated with ribonucleoprotein particles. | in chick embryo fibroblasts transformed by rous sarcoma virus (rsv) the tyrosine phosphorylation of a cellular protein of 34,000 daltons mol. wt. (34 kd) is greatly enhanced; this was shown to be catalyzed by the phosphotransferase activity of rsv transforming protein pp60src. we report here that in cytoplasmic extracts of both normal and transformed cells, in the presence of magnesium ions, the majority of the 34-kd protein is associated with large structures and that a fraction of 34 kd appear ... | 1983 | 11894943 |
| influence of b complex versus non b genetic background on cellular resistance and on rous sarcoma virus-induced tumour regression in chickens. | six of eight inbred lines, segregating for the same b complex alleles, permitted a comparison of genetic backgrounds on resistance to bryan high titre rous sarcoma virus (rsv) of subgroups a, b and c. tests were based both on wing web challenge (wwc), using 682 five-week-old chicks, and on the chorioallantoic membranes (cams) of 606 twelve-day-old embryos (cam test). absence of tumour development on wwc and of pock formation on cams indicated cellular resistance to rsv (primary mechanism of gene ... | 1983 | 18766787 |
| comparison of protein phosphorylations in variant a431 cells with different growth responses to epidermal growth factor. | growth of a selected variant of a431 cells (clone 29) is stimulated by epidermal growth factor (egf) in contrast to the growth inhibition caused by egf in an unselected clone, a431(8). twelve phosphoproteins from each clone were compared to determine whether unique egf-dependent substrate phosphorylations might explain the cells' differing growth responses to egf. treatment of both clone 29 and a431(8) cells with egf increased phosphorylation of the egf receptor/kinase and six cellular proteins ... | 1984 | 6202705 |
| inability of rous sarcoma virus to cause sarcomas in the avian embryo. | the injection of rous sarcoma virus (rsv) into the wing web of newly hatched chicks causes a rapidly growing sarcomatous tumour which is palpable within 1 week of inoculation; and cultures of fibroblasts derived from chick embryos (cef) and infected with rsv become rapidly transformed. genetic studies have determined that expression of a single viral gene, designated v-src, is necessary for neoplastic transformation. this gene codes for a 60,000-molecular weight phosphoprotein termed pp60spc , w ... | 1984 | 6203040 |
| characterization of a replication-defective temperature-sensitive mutant of rous sarcoma virus. | a temperature-sensitive mutant (la83) of rous sarcoma virus defective both in the transformation and replication function has been isolated and partially characterized. temperature-shift experiments showed that the defects in both the focus-forming and replication functions were late and continuous. the mutant la83 was complemented by avian leukosis viruses. complementation of la83 replication was also observed with the glycoprotein-deletion mutant, brian high-titer rsv(-) suggesting that the en ... | 1984 | 6204447 |
| isolation of proteins with kinase activity and related to pp60 src from human cells. | a protein kinase activity (pk) was associated with immunoprecipitates between polypeptides of human lymphoblastoid cells of malignant origin (raji cell line) or of their normal counterparts ( priess cell line) and antibodies directed against avian pp60 src or against the carboxyterminal hexapeptide of pp60 src. therefore, these human cells and rous sarcoma virus (rsv) transformed avian cells share antigenic determinants of pp60 src and, in particular, its carboxyterminal sequence, as well as one ... | 1984 | 6204646 |
| monoclonal antibodies for the rapid diagnosis of respiratory syncytial virus infection by immunofluorescence. | eight separate monoclonal antibodies to the long strain of respiratory syncytial virus (rsv) were tested for their utility as rapid diagnostic reagents in immunofluorescence. preliminary screening indicated that all 8 reacted with 11 field strains from three previous local rsv outbreaks and with 4 of 5 additional strains chosen because of their antigenic diversity by neutralization. two monoclonal antibodies, one each directed against a surface glycoprotein and the nucleocapsid protein, were the ... | 1984 | 6204807 |
| immunofluorescence localization of phosphotyrosine containing proteins in rsv-transformed mouse fibroblasts. | the localization of phosphotyrosine-containing proteins (p-tyr proteins) was studied by immunofluorescence microscopy employing affinity-purified azobenzyl phosphonate (abp) antibodies, which specifically cross-react with phosphotyrosine. in rous sarcoma virus (rsv)-transformed fibroblasts, after fixation followed by permeabilization with nonionic detergents, abp antibodies gave a diffuse staining of the cytoplasm and specifically decorated restricted areas of the ventral plasma membrane corresp ... | 1984 | 6205889 |
| monoclonal antibodies specific for the virion polypeptide, p27, of avian retroviruses. | the major component of the core structure of avian sarcoma leukosis viruses is a 27 kd molecular weight polypeptide, p27. spleen cells from mice immunized with the schmidt-ruppin strain of rous sarcoma virus (rsv) were fused with mouse myeloma cells (sp2/0), and hybridoma cell lines producing monoclonal antibodies to p27 were isolated. the monoclonal antibodies were all of the igg1 subclass with kappa light chains. these antibodies immunoprecipitated p27 and its precursor proteins from extracts ... | 1984 | 6205983 |
| tyrosyl kinases acquired from anchorage-independent cells by a membrane-enveloped virus. | tyrosyl kinase activity in vesicular stomatitis virus (vsv) acquired from host cells that differ in morphology was investigated. vsv grown in baby hamster kidney (bhk) cells with rounded morphology and a high efficiency of colony formation in soft agar (rous sarcoma virus [rsv]-transformed and suspension bhk cells) was compared with vsv grown in bhk cells with a flattened morphology and lower efficiency of colony formation in soft agar (rsv-infected revertant and control bhk cells). tyrosyl kina ... | 1984 | 6206078 |
| preparation and characterization of antibodies to o-phosphotyrosine and their use for identification of phosphotyrosine-containing proteins. | to facilitate the identification of phosphotyrosine (p-tyr)-containing proteins, rabbit polyclonal antibodies and mouse monoclonal antibody specifically reactive to p-tyr were prepared by hyperimmunizing the animals with p-tyr-conjugated bovine serum albumin or poly-l-lysine. as determined by a solid-phase radioimmunoassay and an enzyme-linked immunosorbent assay, the antibodies reacted with p-tyr-conjugated target antigens but not with those conjugated with phosphoserine (p-ser) or phosphothreo ... | 1984 | 6210255 |
| defective regulation of immune responses in respiratory syncytial virus infection. | the relationship of suppressor cell numbers and function to virus-specific ige response was determined in 72 infants with respiratory syncytial virus (rsv) infection. monoclonal antibodies to membrane antigens were used to enumerate okt4 and okt8 antigen-positive cells, and suppressor cell function as quantitated by the degree of suppression of lymphocyte mitogenesis induced by incubation of lymphocyte cultures with histamine. patients with bronchiolitis had fewer okt8-positive cells during conv ... | 1984 | 6236259 |
| detection of phosphotyrosine-containing proteins in the detergent-insoluble fraction of rsv-transformed fibroblasts by azobenzene phosphonate antibodies. | the rous sarcoma virus (rsv) oncogene product pp60src is known to trigger the acquisition of the transformed phenotype by phosphorylating host cell target molecule(s) at tyrosine residues. to identify phosphotyrosine-containing proteins, rabbit antibodies were raised against the synthetic hapten p-azobenzene-phosphonate (abp) that specifically cross-reacts with phosphorylated tyrosine. by immuno-decoration of proteins extracted from rsv-transformed mouse fibroblasts and transferred to nitrocellu ... | 1984 | 6201361 |
| differentiation and transformation of neural plate cells. | the developmental potential of presumptive neural plate cells of prestreak chick embryos (stage 1) and neural plate cells from definitive streak chick embryos (stage 4) has been examined in cell culture using specific markers that identify the major cell types in the vertebrate central nervous system. the prestreak presumptive neural plate (pspnp) cells, stage 1, assume an epithelial appearance in vitro and synthesize cellular fibronectin, but do not express markers for the neuronal, astrocytic, ... | 1984 | 6201407 |
| monoclonal antibodies protect against respiratory syncytial virus infection in mice. | twenty-five monoclonal antibodies (mab) to respiratory syncytial virus (rsv) and two to hepatitis b virus were inoculated intravenously into mice. twenty-four hours later the mice were challenged intranasally with rsv. eleven of 14 mab against fusion protein and four out of six mab against a larger glycoprotein (gp84) significantly reduced the titre of rsv in the lungs when mice were killed 5 days later. five mab against three other rsv proteins and two mab against hepatitis b virus had no signi ... | 1984 | 6201436 |
| the characterization and uses of monoclonal antibodies to respiratory syncytial virus. | fifteen hybridomas secreting monoclonal antibodies against respiratory syncytial virus (rsv) have been produced. three react with nucleoprotein (mw 42 000 daltons), 1 with phosphoprotein (mw 36 000 daltons), 1 with a larger protein (mw 60 000 daltons) and 10 with the fusion glycoprotein (mw 46 000 + 22 000 daltons). by immunofluorescent staining of infected cells, 5 monoclonal antibodies bind to cytoplasmic inclusions and the remainder give diffuse cytoplasmic fluorescence. virus infectivity is ... | 1984 | 6084614 |
| role of cell division in differentiation of myoblasts infected with a temperature-sensitive mutant of rous sarcoma virus. | the relationship between a potential requirement for cell dna synthesis and the expression of differentiated muscle cell functions was investigated using primary chicken embryo myoblasts infected with a temperature-sensitive mutant of rous sarcoma virus (rsv). under optimized conditions, transformed myoblasts growing at the permissive temperature could differentiate into multinucleated myotubes, express muscle-specific myosin, desmin and acetylcholine receptors in the absence of dna synthesis an ... | 1984 | 6086316 |
| multiple enhancer domains in the 3' terminus of the prague strain of rous sarcoma virus. | the 3' terminal region of the prague strain of rous sarcoma virus (prrsv) contains at least three distinct domains that comprise two functional enhancer elements. two of these domains (designated b and c) are found in the u3 region of the 3' long terminal repeat (ltr) while the third (designated a) is located in the sequences immediately preceding the ltr termed xsr sequences. combinations of adjacent domains [e.g., (a + b or b + c)] are capable of activating the expression of the sv40 early pro ... | 1984 | 6089114 |
| characterization of cryptovirogenic, virus-productive and helper-dependent virogenic hamster tumour cell lines. | three hamster cell lines were established from tumours induced with the virus rescued from xc cells. the h-20 cell line produces little infectious transforming virus and synthesizes uncleaved pr76 (product of gag gene) in the amount comparable to that obtained from pr-rsv-c-infected permissive cells. like the helper-dependent virogenic h-18 cell line, it however produces only a marginal amount of viral glycoprotein (env gene product) as revealed by the complementation assay. even a lower amount ... | 1984 | 6090228 |
| different importance of primary lymphoid organs for rous sarcoma regression in inbred lines of chickens. | resistance to progressive growth of tumours induced by rous sarcoma virus is controlled mainly by genes linked to the b complex (the major histocompatibility complex) of the chicken. the effect of thymectomy and bursectomy on this resistance was studied in chickens from highly inbred lines and their f1 hybrids which differ by the genotype at the b complex and by the degree of genetically controlled resistance or susceptibility. the results show that thymectomy has considerable effect on growth o ... | 1984 | 6090231 |
| genetic effects of injection of rous sarcoma virus dna into polar plasm of early drosophila melanogaster embryos. | retroviral proviruses and the transposable elements of eukaryotic genomes are structurally similar. the biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjacent genes and cause their transposition. it is of interest to determine whether retroviral proviruses have the same mutagenic and gene transposing ability as transposable elements, particularly because the ... | 1984 | 6090937 |
| detection of virus-specific sequences in drosophila melanogaster mutants induced by injection of rsv dna into early embryos. | the injection of purified rous sarcoma virus (rsv) (prague strain) into drosophila melanogaster (oregon r line) eggs changes the fly phenotype in certain cases, and rsv-specific sequences can be identified in the drosophila genome (ref. 1 and preceding paper). here we have used southern blotting to analyse in greater detail the proviral dna present in several mutant lines of d. melanogaster produced by microinjection of intact rsv or plasmid dna containing the viral insert. in certain population ... | 1984 | 6090938 |
| hormonal regulation of the rous sarcoma virus src gene via a heterologous promoter defines a threshold dose for cellular transformation. | we have derived rat cell lines producing different and regulatable amounts of pp60v-src by introducing the src gene of rous sarcoma virus (rsv) under the control of the glucocorticoid-responsive transcriptional promoter from the mouse mammary tumor virus (mmtv). we find that the cellular phenotype is strictly dependent upon the dose of pp60v-src with a distinct threshold for changes indicative of neoplastic potential. cells with low constitutive levels of pp60v-src are not phenotypically disting ... | 1984 | 6091899 |
| [induction of unstable mutations in drosophila melanogaster by the microinjection of oncogenic viruses and their dna into early embryos]. | rna-containing raus sarcoma virus, recombinant plasmid pbr322 inserted by kdna rsv (pprc11) and sa7 adenovirus dna were injected into the polar region of drosophila melanogaster early embryons. the exogenic genetic material injected was shown to induce mutations, many of them unstable. in a number of cases, virus-specific sequences were found in dna isolated from mutant flies. it is hypothesized that mutations induced by dna of oncogenic viruses are of the insertion type. | 1984 | 6092212 |
| evidence for two distinct c-src loci on human chromosomes 1 and 20. | a number of proto-oncogenes have recently been localized to the chromosomal segments that are the breakpoints in the specific rearrangements noted in human malignant diseases. moreover, rearranged forms of several proto-oncogenes have been identified in malignant cells; in several instances, the proto-oncogene has undergone an alteration as a result of a nonrandom chromosomal rearrangement. one proto-oncogene that has yet to be associated with human neoplastic disease is c-src, the cellular homo ... | 1984 | 6092965 |
| phenotypic changes and loss of n-cam-mediated adhesion in transformed embryonic chicken retinal cells. | transformation of 6-d-old embryonic chicken retinal cells by rous sarcoma virus (rsv) was found to cause significant changes in several cellular properties including adhesiveness, motility, and state of differentiation. the alterations in cell adhesivity were analyzed by means of specific antibodies to the calcium-independent neural cell adhesion molecule, n-cam. in the rsv-transformed cells the amount of n-cam present at the cell surface was significantly decreased relative to normal cells, as ... | 1984 | 6094590 |
| mutations of the rous sarcoma virus env gene that affect the transport and subcellular location of the glycoprotein products. | the envelope glycoproteins of rous sarcoma virus (rsv), gp85 and gp37, are anchored in the membrane by a 27-amino acid, hydrophobic domain that lies adjacent to a 22-amino acid, cytoplasmic domain at the carboxy terminus of gp37. we have altered these cytoplasmic and transmembrane domains by introducing deletion mutations into the molecularly cloned sequences of a proviral env gene. the effects of the mutations on the transport and subcellular localization of the rous sarcoma virus glycoproteins ... | 1984 | 6094591 |
| integration and expression of several molecular forms of rous sarcoma virus dna used for transfection of mouse cells. | to assess the factors required for integration and expression of retroviral dna, we have examined viral dna, rna, and protein in nih/3t3 mouse cells transformed by transfection with various forms of cloned rous sarcoma virus (rsv) dna. linear rsv dna molecules, derived from circular dna containing two long terminal repeats (ltrs) and permuted by cleavage at the saci restriction endonuclease site in the leader sequence, were integrated near the ends of the linear molecule, with the ltrs on the 3' ... | 1984 | 6095056 |
| an assay for transient gene expression in transfected drosophila cells, using [3h]guanine incorporation. | we have developed an assay for transient gene expression using a dominant-selectable marker previously employed to transform drosophila cultured cells. drosophila hydei cells transfected with a functional escherichia coli xanthine guanine phosphoribosyl transferase gene (gpt), under the control of the long terminal repeats (ltrs) of the copia transposable element, rapidly incorporate guanine into acid-precipitable counts. autoradiographic analysis in situ shows that approximately 20% of cells ta ... | 1984 | 6096129 |
| avian erythroblastosis virus e26: nucleotide sequence of the tripartite onc gene and of the ltr, and analysis of the cellular prototype of the viral ets sequence. | an intact 5.7-kb provirus of the avian erythroblastosis virus e26 has been molecularly cloned for comparisons with avian myeloblastosis virus (amv) and other avian tumor viruses. e26 and amv transform hemopoietic cells exclusively. both cause myeloblastosis, but e26 also causes erythroblastosis. sequence analysis of the proviral dna showed that: the tripartite transforming gene of e26 forms a contiguous reading frame of 1046 codons, including 272 gag, 283 mybe, and 491 ets codons. no subgenomic ... | 1984 | 6097027 |
| variations of endogenous chicken proviruses: characterization of new loci of endogenous proviruses in the genome of italian partridge chickens. | the composition and structure of endogenous proviruses present in the genome of italian partridge chickens were studied by the method of blot hybridization using rav-2 [32p]dna or ltr of rsv as hybridization probes. the genomes of 5 out of 39 chickens analyzed did not contain endogenous proviruses related to rav-2. different sets of five so far undescribed endogenous proviruses, differing in the structure and location, were detected in the dna of other ip chickens. none of them is identical in i ... | 1984 | 6097480 |
| [isolation of virus-specific double-stranded rna from cells transformed with rous sarcoma virus]. | several methods were used for isolation of double-stranded (ds) rna from the cytoplasm of rous sarcoma virus-transformed chick embryo cells. the dsrna was shown to have a high melting temperature (82.5 degrees c) in 0.16 m phosphate buffer (ph 6.8), which shifted to more than 90 degrees c after rnase treatment. the size of a single strand was approximately 1300-1600 nucleotides and rnase-resistant fragments were 50-250 nucleotides long. double-stranded rna formed hybrids with the labeled genomic ... | 1984 | 6097811 |
| a non-mhc genetic influence on response to rous sarcoma virus-induced tumors in chickens. | avian-leukosis-free females from regional poultry research laboratory (rprl) inbred lines 6(1) and 7(2) were inseminated with pooled semen from rprl line 15i5 males. the progeny resulting from the two crosses 15i5 x 6(1) and 15i5 x 7(2) were of the b2/b15 major histocompatibility complex (mhc) genotype, the b2 haplotypes from lines 6(1) and 7(2) being indistinguishable. when challenged with rous sarcoma virus (rsv) at 6 weeks of age, 30 out of 31 progeny of cross 15i5 x 6(1) developed tumors tha ... | 1984 | 6098253 |
| enzymatic characteristics of pp60v-src isolated from vanadium-treated transformed cells. | the transforming protein of rous sarcoma virus (rsv) typically appears as a single phosphorylated polypeptide designated pp60v-src. pp60v-src possesses a protein kinase activity specific for tyrosine residues on select protein substrates. treatment of rsv-transformed cells with vanadium ions resulted in the appearance of an electrophoretic variant of pp60v-src and was paralleled by a significant increase in the src kinase specific activity in purified enzyme preparations. both the normal (standa ... | 1984 | 6098587 |
| complete env gene deletions of three replication-defective strains of rous sarcoma virus and a model for the origin of their genetic structures. | replication-defective deletion mutants of rous sarcoma virus (rsv) have been described which transform cells in culture and elaborate envelope (-) defective particles. the env deletions of two clonal variants of the bryan strain of rsv, rsv(-)3, and rsv(-)16, and of a replication-defective variant of schmidt-ruppin rsv (srn8) were analyzed by fingerprinting oligonucleotides hybridized by a molecularly cloned env dna probe that spans from near the 3' end of pol to the 3' end of env. it was observ ... | 1984 | 6100578 |
| infection of gnotobiotic calves with a bovine and human isolate of respiratory syncytial virus. modification of the response by dexamethasone. | a bovine and a human strain of rsv both adapted to bovine cell culture, have been inoculated separately into 13 and 7 gnotobiotic calves respectively by 3 different methods. both strains infected calves and showed similar growth patterns. virus was recovered from the nasopharynx between one and 11 days with peak titres between 3 and 8 days following inoculation. with the exception of 4 calves treated with dexamethasone no clinical signs and only minimal macroscopic lesions of the lung were induc ... | 1984 | 6365036 |
| experimental pneumonia in gnotobiotic calves produced by respiratory syncytial virus. | a bovine isolate of respiratory syncytial virus (rsv), when inoculated intranasally into eight gnotobiotic calves produced significant macroscopic lesions of the lung (2-25% consolidation) but failed to produce any clinical signs of disease. the microscopic lesions comprised proliferative and exudative bronchiolitis with accompanying alveolar collapse and infiltration by mononuclear cells of the peribronchiolar tissue and alveolar walls. virus was recovered from the nasopharynx between days 2 an ... | 1984 | 6365144 |
| evidence for both respiratory syncytial virus and measles virus antigens in the osteoclasts of patients with paget's disease of bone. | recent ultrastructural and immunohistochemical evidence supports the hypothesis that paget's disease of bone is a slow viral infection of the paramyxoviridae family. conflicting evidence for the presence of respiratory syncytial virus (rsv), a pneumovirus, or measles, a morbillivirus, has been reported. by the indirect fluorescent antibody assay, four rsv antisera were compared with four measles antisera on serial sections of pagetic bone or replicate coverslips of cells from pagetic bone grown ... | 1984 | 6365389 |
| viral diagnoses using the rapid immunofluorescence technique and epidemiological implications of acute respiratory infections among children in different european countries. | from november 1978 to october 1981, a total of 7716 specimens of nasopharyngeal secretions were examined by the rapid immunofluorescence technique to determine the frequency of infections caused by the respiratory syncytial virus (rsv), influenza virus a, and parainfluenza viruses 1 and 3. the tests were carried out in six different virus laboratories located in newcastle upon tyne (england), copenhagen, oslo, stockholm, turku (finland), and vienna; laboratories in lisbon and paris participated ... | 1984 | 6375886 |
| isolation of pathogenic bacteria from the nasopharynx of children with respiratory syncytial virus infection. predictive value of chest roentgen examination and laboratory tests. | potentially pathogenic bacteria were isolated from the nasopharynx in 33/66 hospitalized infants and children with verified respiratory syncytial virus (rsv) infection. the value of chest roentgenograms and blood counts for the prediction of concomitant bacterial infection was evaluated. abnormal chest roentgenograms were found in 89% whether pathogenic bacteria were present or not. the most common finding was general hyperinflation of the lungs, with or without infiltrates. this occurred more f ... | 1984 | 6377479 |
| respiratory virus infection in hospitalized children in austria 1979-1982. diagnosis by immunofluorescence. | nasal secretions from 349 austrian children under six years of age who were hospitalized for respiratory illnesses were screened for the presence of respiratory syncytial virus (rsv), parainfluenza virus 1 and 3, adenovirus and influenza a virus over a period of four years by the immunofluorescence technique. 35% of the specimens were found to be positive for one of the five viruses investigated. rsv was detected in 31% of the nasal secretions and was thus the most frequently encountered causati ... | 1984 | 6381315 |
| pathogenesis of experimental bovine respiratory syncytial virus infection in sheep. | conventionally reared lambs, seronegative to bovine respiratory syncytial virus (rsv), were inoculated with bovine rsv by an aerosol route. a mild clinical response, characterized by transient fever at postinoculation day (pid) 4, occurred and was accompanied by serous oculonasal secretions in less than 50% of the infected lambs. a decrease in total wbc was observed on pid 6 and 11, with a reduction in the total numbers of lymphocytes. bovine rsv was recovered from nasal secretions of inoculated ... | 1984 | 6383151 |
| interaction of bovine respiratory syncytial virus and pasteurella haemolytica in the ovine lung. | the potential synergistic effect of bovine respiratory syncytial virus (rsv) and pasteurella haemolytica in the production of pneumonia after aerosol/intranasal infection of conventionally reared lambs was evaluated. a mild clinical response was observed in lambs given virus and/or bacteria. gross pulmonary lesions were seen in 3 of 6 lambs given rsv and then p haemolytica 3 or 6 days later, respectively (groups d and e), and in 1 lamb of 5 given virus and bacteria simultaneously (group g). gros ... | 1984 | 6383152 |
| immune response to respiratory syncytial virus: prevention of syncytia formation by human serum during in vitro infection. | human serum specimens containing respiratory syncytial virus (rsv)-specific neutralizing antibody were found to prevent the formation of syncytia when applied to hep-2 tissue culture monolayers which had been infected with rsv 12 h previously. this was evidenced by the demonstration of rsv-infected cells without any syncytia formation in the monolayers treated with rsv antibody-positive serum. on the other hand, widespread syncytia formation was observed with antibody-negative control serum. the ... | 1984 | 6387611 |
| processing of gpr92env, the precursor to the glycoproteins of rous sarcoma virus: use of inhibitors of oligosaccharide trimming and glycoprotein transport. | a number of aspects of the processing of gpr92env, the precursor to the viral glycoproteins gp85 and gp35 of rous sarcoma virus (rsv), have been studied. first, the kinetics of gpr92env processing have been examined, revealing that the precursor is overproduced in the infected cell and only a small percentage (less than 5%) is converted into mature glycoprotein in virus particles. second, the effects of inhibitors of intracellular transport (monensin) and oligosaccharide trimming (n-methyl-1-deo ... | 1984 | 6320537 |
| study of pp60v-src protein kinase activity in synchronized chicken embryo fibroblasts infected with rous sarcoma virus. | chicken embryo fibroblast (cef) cultures, synchronized by the addition of serum to stationary cells, were exposed to schmidt-ruppin strain of rous sarcoma virus (sr-rsv) and the appearance of pp60v-src protein kinase activity was examined through the cell cycle. in cells infected either at the beginning or at the end of g1, the onset of pp60v-src protein kinase activity was coincidental, closely following mitosis, with a delay between the infection of cells with sr-rsv and the appearance of prot ... | 1984 | 6319162 |
| unphosphorylated gelsolin is localized in regions of cell-substratum contact or attachment in rous sarcoma virus-transformed rat cells. | regions associated with cell-substratum contact or attachment in rous sarcoma virus (rsv)-transformed rat fibroblasts (rr1022 cells) were identified by reflection-interference microscopy. electron microscopy of such regions revealed the presence of discrete membrane-associated structures composed of a paracrystalline lattice of hexagons and pentagons to which actin filaments appear to be attached. staining of actin by biotin-labeled heavy meromyosin showed that transformed cells, unlike normal f ... | 1984 | 6319434 |
| dna sequence of the bryan high-titer strain of rous sarcoma virus: extent of env deletion and possible genealogical relationship with other viral strains. | the genetic structure of the bryan high-titer strain of rous sarcoma virus (bh-rsv) was analyzed by using a molecular clone obtained from proviral dna. dna sequencing of the pol-src junction of bh-rsv revealed that the env sequence was almost entirely absent; only six base pairs following the pol termination codon remained. beginning at nucleotide 7 (relative to the end of pol), a 91-base pair sequence identical to the 91 base pairs immediately upstream from src in other strains of rsv was found ... | 1984 | 6319754 |
| independent expression of common and private tumor-specific cell surface antigens in somatic cell hybrids between rous sarcoma virus-transformed and normal cells. | somatic cell hybrids were prepared by fusion of a rous sarcoma virus (rsv)-induced mouse tumor (csa1m cl-11) with a chinese hamster embryo lung fibroblast line (wg3h x agr x ouar). some hybrid clones showed rapid anchorage-independent growth and intracellular production of rsv-pp60src. other hybrid clones showed slow anchorage-dependent growth and no production of such a protein. the former expressed both a common tumor-specific cell surface antigen(s) (tssa) shared with other rsv-induced tumors ... | 1984 | 6319792 |
| bovine respiratory syncytial virus infections in young dairy cattle: clinical and haematological findings. | clinical signs and haematological findings during the acute and convalescent phases of an infection with bovine respiratory syncytial virus (rsv) were studied under field conditions. the study was carried out in 139 cattle less than 16 months of age in 16 herds with serologically proven bovine rsv infections. blood was collected for serological and haematological examination. repeated clinical examinations were carried out until 35 days after appearance of disease. signs of general disease such ... | 1984 | 6702077 |
| respiratory syncytial virus-related apnea in infants. demographics and outcome. | medical records of 261 hospitalized patients less than 1 year old with documented respiratory syncytial virus (rsv) infection were reviewed to determine the incidence of rsv-associated apnea and the accompanying risk of subsequent apnea or death. apnea in association with rsv infection occurred in 18% of the infants. premature birth and a young postnatal age were risk factors for development of apnea with rsv disease. apnea of prematurity appeared to be a significant risk factor for rsv apnea de ... | 1984 | 6702769 |
| purification and characterization of gp90, one of the envelope glycoproteins of respiratory syncytial virus. | the large glycoprotein, gp90, of respiratory syncytial virus (rsv) was purified by affinity chromatography using a monoclonal antibody. hyperimmune rabbit antiserum directed specifically to the purified gp90 neutralized rsv to high titre but did not inhibit fusion of previously infected cells. 125i-labelled gp90 bound rapidly to hep-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum. | 1984 | 6707612 |
| infection rates of respiratory syncytial virus in pediatric patients attending phra mongkutklao hospital, bangkok. | respiratory syncytial virus (rsv) and other pathogens were isolated from nasopharyngeal secretions from 200 pediatric patients attending the out patient department of phra mongkutklao hospital with symptoms of upper respiratory tract infections. their sera were also taken for determination of class specific immunoglobulin antibody titers. the positive isolation rates were 36% for rsv, 5.5% for adenovirus 1.5% for herpes simplex virus (hsv), and 4% for staphylococcus aureus. one to 5.5% of these ... | 1984 | 6740381 |
| virus-induced complement activation and neutrophil-mediated cytotoxicity against respiratory syncytial virus (rsv). | complement-dependent neutrophil-mediated cytotoxicity (cdnc) was determined by specific release of 51-chromium (51cr) from respiratory syncytial virus infected hep2 cells in a microcytotoxicity assay. there was significant release of 51cr from rsv infected cells as compared to uninfected cells in the presence of complement (c) and neutrophils (pmn). the degree of cytotoxicity was dependent upon the concentration of c used in the assay. such cytotoxicity was effectively abolished after heat-inact ... | 1984 | 6744659 |
| diagnosis of respiratory syncytial virus infection in children: comparison of viral antigen detection and serology. | direct detection of viral antigen in nasopharyngeal secretion by radioimmunoassay was compared with serology by igg antibody enzyme immunoassay for diagnostic efficacy in 77 children with clinically suspected respiratory syncytial virus (rsv) infections. antigen detection gave a positive diagnosis in 26 of 33 (79%) children in whom rsv infection was diagnosed by any of the two methods. the diagnostic efficacy of antigen detection was dependent upon the interval after onset at which specimens wer ... | 1984 | 6747612 |
| immunoglobulin class-specific antibody response in respiratory syncytial virus infection measured by enzyme immunoassay. | immunoglobulin class-specific enzyme immunoassay (eia) was used for determination of antibody responses in sera collected from 26 children with acute primary respiratory syncytial virus (rsv) infections. all 26 patients had igg antibody responses with a significant titer increase in 24 (92%); an igm antibody response was detected in 19 of the 26 (73%). from patients aged 6 months or less only 5 of 8 produced detectable igm antibodies, whereas all patients aged 1-2 years did so. igm antibodies ap ... | 1984 | 6747613 |
| butyrate reversibly arrests the proliferation of normal and rous sarcoma virus-infected chicken heart mesenchymal cells. | sodium butyrate at 5 x 10(-3) m reversibly arrests the proliferation of both normal and rsv-infected chicken heart mesenchymal cells. this finding suggests that butyrate acts by causing non-specific inhibition of cell proliferation, rather then by specifically suppressing the neoplastic phenotype or by inducing recovery of cells from the neoplastic state. | 1984 | 6321872 |
| monoclonal antibodies specific for the avian sarcoma virus transforming protein pp60src. | monoclonal antibodies to avian sarcoma/leukosis virus-coded transforming, structural, and polymerase proteins have been developed. in this first communication, a monoclonal antibody to pp60src will be characterized. the antibody can bind to pp60src in radioimmunoassays, precipitates this antigen in protein kinase tests, and demonstrates in immunofluorescence investigations that pp60src has different intracellular localizations depending on whether rsv-transformed avian or mammalian cells are stu ... | 1984 | 6322430 |
| treatment of respiratory viral infection in an immunodeficient infant with ribavirin aerosol. | an infant with severe combined immunodeficiency syndrome (scids) secondary to adenosine deaminase deficiency had pneumonitis and combined infection with respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3). four separate courses of ribavirin were delivered by small-particle aerosol. the piv3 disappeared during the first course, and rsv disappeared after the fourth course on the 58th hospital day. neither virus returned during profound immunosuppression for bone marrow transpla ... | 1984 | 6322573 |
| cytoskeleton and adhesion patterns of cultured chick embryo chondrocytes during cell spreading and rous sarcoma virus transformation. | the cytoskeleton and the adhesion complex of chick embryo chondrocytes maintained in vitro have been studied by fluorescence and interference reflection microscopy during the process of cell spreading. the pattern of actin-containing microfilaments and the distribution of vinculin speckles on adhesion plaques have been found to change as a function of the culture time. newly plated chondrocytes adhere to the substratum mostly around a peripheral ring-like region and show a complex tridimensional ... | 1984 | 6323198 |
| density-dependent inhibition of growth: inhibitory diffusible factors from 3t3- and rous sarcoma virus (rsv)-transformed 3t3 cells. | we recently fractionated, from the culture medium of 3t3 cells, a thermolabile inhibitory diffusible factor (idfn) with a molecular weight of about 40,000 daltons, which decreased nucleic acids synthesis of stimulated target 3t3 cells. in the present publication the inhibitory activities of idfn (produced by 3t3 cells) and idft (produced by rsv-transformed 3t3 [3t3 sra/h] cells) on 3t3 and 3t3 sra/h cells have been compared. the inhibitory activity of idfn decreased (by a mean of 57%) when it wa ... | 1984 | 6323487 |
| it is rous sarcoma virus protein p12 and not p19 that binds tightly to rous sarcoma virus rna. | the interactions between rous sarcoma virus (rsv) rna and the viral proteins in the virus have been analysed by sen & todaro (1977) using ultraviolet light irradiation; they showed that the major protein ultraviolet light cross-linked to the viral rna was p19 as identified by polyacrylamide gel electrophoresis. we report here that it is not viral protein p19 but p12 that binds tightly to rsv rna upon ultraviolet light irradiation of the virus. therefore, the binding sites of the viral protein al ... | 1984 | 6323722 |
| hematopoietic cell transformation by a murine recombinant retrovirus containing the src gene of rous sarcoma virus. | a recombinant murine retrovirus (mrsv) containing the src gene of avian rous sarcoma virus (rsv) was shown to induce hematopoietic colonies in infected mouse bone marrow. mrsv-induced colony formation followed single-hit kinetics and required mercaptoethanol in the agar medium. cells from the colonies induced by mrsv could be established as continuous cell lines that demonstrated unrestricted self-renewal in vitro and tumorigenicity in vivo. the transformants, all of which expressed high levels ... | 1984 | 6326123 |
| an in vitro screening method for antitumor and/or antitumorigenic substances involving the transformation of chick embryo fibroblasts infected with rous sarcoma virus. | an efficient in vitro screening method for antitumor and/or antitumorigenic substances was established. the method is based on determining inhibitory effect of a compound on an rsv-induced tumorigenic process and the growth of the established tumor cells in a single assay system in the presence of normal cells. these effects were determined by inhibition of focus formation in a culture of chick embryo fibroblasts, while the nonspecific cytotoxic effect was determined by inhibition of the protein ... | 1984 | 6327589 |
| dnase i sensitivity of the alpha 2(i) collagen gene: correlation with its expression but not with its methylation pattern. | the chromatin structure of the chick alpha 2(i) collagen gene was probed with dnase i. because our previous work strongly suggested that the 5' end of this gene is not methylated whereas the rest of the gene is methylated whether or not the gene is expressed, we compared the relative dnase i sensitivity of the methylated and unmethylated segments. both regions demonstrate similar relative dnase i sensitivities within a given tissue. in chromatin of chick embryo fibroblasts, we find a dnase i hyp ... | 1984 | 6328424 |
| cytoskeletal changes induced by two avian sarcoma viruses: ur2 and rous sarcoma virus. | ur2-transformed cells were examined by immunofluorescence and compared to control cells and cells transformed by rous sarcoma virus (rsv). actin and tubulin which are normally depolymerized in rsv-transformed cells appeared to be unaffected by ur2 transformation. cell surface fibronectin which is normally lost from rsv-infected cells, appears more abundantly on ur2-transformed cells than on normal cells. vinculin was shown to be in adhesion plaques in ur2-transformed cells as well as in control ... | 1984 | 6330996 |
| pp60c-src kinase is in chick and human embryonic tissues. | the normal cellular protein pp60c-src is a tyrosine-specific protein kinase that is homologous to the transforming protein of rous sarcoma virus (rsv) but its function is unknown. the expression of pp60c-src in chick and human embryonic tissues was monitored by the immune complex protein kinase assay, western transfer analysis, and immunocytochemical staining at the light microscope level. pp60c-src kinase was expressed in the head and trunk regions of the chick embryo at all stages of developme ... | 1984 | 6428952 |
| direct evidence that oncogenic tyrosine kinases and cyclic amp-dependent protein kinase have homologous atp-binding sites. | p60src, the transforming protein of rous sarcoma virus (rsv), is a protein kinase that has a strict specificity for tyrosine. the phosphorylation of cellular proteins by p60src (ref. 4) results in transformation. recently, barker and dayhoff discovered that residues 259-485 of p60src have 22% sequence identity with residues 33-258 of the catalytic subunit of cyclic amp-dependent protein kinase, an enzyme that has a specificity for serine. because it was necessary to introduce eight gaps to align ... | 1984 | 6431300 |
| characterization of a transformation-sensitive protein in the extracellular matrix of chicken embryo fibroblasts. | the extracellular matrix of cultured chicken embryo fibroblasts undergoes a number of modifications during the early stages of oncogenic transformation. one alteration is increased production of a small protein (mr approximately 21,000) which is transiently deposited in the matrix by transforming cells infected with la24, a temperature-sensitive mutant of rous sarcoma virus (rsv) (blenis, j., and hawkes, s.p. (1983) proc. natl. acad. sci. u.s.a. 80, 770-774). this protein is a major component of ... | 1984 | 6432799 |
| respiratory viruses and mycoplasma pneumoniae infections at the time of the acute exacerbation of chronic otitis media. | the present study was undertaken to ascertain whether or not patients with chronic otitis media are infected with viruses or mycoplasma at the time of sudden increase in otorrhea. from 26 patients with acute exacerbation of chronic otitis media, sera were collected at the time of sudden increase in otorrhea and three to four weeks later. these paired sera were examined for antibody titer to respiratory viruses (21 species) and mycoplasma pneumoniae. of them, influenza b virus and rsv infections ... | 1984 | 6442134 |
| long-term prospective study in children after respiratory syncytial virus infection. | we have prospectively evaluated for the past 8 years 29 children who were hospitalized during infancy with acute lower respiratory tract illness caused by respiratory syncytial virus (rsv). no differences in the prevalence of a family history of atopy or breast-feeding in these infants compared with controls were noted. however, a history of parental smoking was significantly associated with hospital admission for rsv lower respiratory tract disease. evidence of atopy, as defined by serum ige le ... | 1984 | 6470859 |
| changes in ganglioside metabolism during in vitro differentiation of quail embryo myoblasts. | the metabolism of gangliosides was studied during the in vitro differentiation of both normal quail myoblasts and myoblasts which have been transformed by a temperature-sensitive mutant of rous sarcoma virus (rsv). these transformed cells can be maintained undifferentiated if incubated at 35 degrees c, but they will differentiate when shifted to 41 degrees c. (d. montarras and m. y. fiszman (1983) j. biol. chem. 258, 3882-3888). the analysis of [14c]glucosamine-labeled gangliosides by two-dimens ... | 1984 | 6479448 |
| interaction of polymorphonuclear leukocytes and viruses in humans: adherence of polymorphonuclear leukocytes to respiratory syncytial virus-infected cells. | the nature of neutrophil-respiratory syncytial virus (rsv) interaction was investigated by assessing factors that influence neutrophil adherence to rsv-infected tissue culture monolayers. the adherence of neutrophils to infected cells was directly proportional to the degree of rsv replication as evidenced by infectious virus production, cytopathological changes, or viral antigen appearance. sixty-one percent of the neutrophils adhered to the rsv-infected cells as compared with 52.7% on noninfect ... | 1984 | 6481852 |
| a comparison of three vaccines against respiratory syncytial virus in calves. | an inactivated vaccine against respiratory syncytial virus (rsv) was compared with two live vaccines. the inactivated (gc) vaccine consisted of glutaraldehyde-fixed bovine nasal mucosa cells persistently infected with rsv and emulsified with oil adjuvant. the live vaccines were a modified virus (mv) derived from a bovine strain of rsv and a temperature-sensitive mutant (ts-1) derived from a human strain. the gc vaccine was inoculated subcutaneously into 12 calves and the live vaccines intramuscu ... | 1984 | 6501875 |
| cellular immune response to infection with respiratory syncytial virus and influence of breast-feeding on the response. | virus-specific lymphocyte transformation (ltf) activity in vitro was examined in 78 infants with various forms of illness due to respiratory syncytial virus (rsv) infection. in 73 subjects with lower respiratory tract disease, significant ltf activity was often detected within one week after onset of initial symptoms, and responses characteristic for each clinical form were observed in the subsequent rise. thus, mean activity in subjects with tracheobronchitis increased gradually, with the maxim ... | 1984 | 6512509 |
| prevalence of antibodies to respiratory syncytial virus in various populations in israel. | a preliminary study on the prevalence of antibodies to respiratory syncytial virus (rsv) in individuals of various age-groups was carried out. in healthy infants aged 6 to 12 months, the prevalence was 27%. it increased to 37 and 54% in children aged 1 to 2 and 2 to 4 years, respectively. the prevalence reached 78% in healthy individuals who were more than 20 years old, including pregnant women. significant levels of specific antibodies were detected in 59% of children aged 6 to 12 months who we ... | 1984 | 6519945 |
| an outbreak of respiratory syncytial virus pneumonia in a nursing home for the elderly. | an outbreak of pneumonia and febrile respiratory illness took place in a los angeles county nursing home for the elderly in february and march of 1979. forty of 101 (40%) residents were affected. twenty-two (55%) had pneumonia and eight (20%) died. the outbreak extended over a period of 6 weeks. serological evidence implicated respiratory syncytial virus (rsv) as the causative agent. three patients had fourfold or greater rises in the titre of antibody to rsv, one had high titres in paired sampl ... | 1984 | 6527041 |
| the development of respiratory syncytial virus-specific immune complexes in nasopharyngeal secretions following natural infection. | sequential samples of nasopharyngeal secretions (nps) from 61 infants and children with respiratory syncytial virus (rsv) infection were examined for the presence of rsv-specific immune complexes in order to determine their possible role in recovery from or pathogenesis of rsv infection. immune complexes in nps were identified by the raji cell assay using indirect immunofluorescence and rsv antigen in these complexes was also detected by indirect immunofluorescence. rsv-specific immune complexes ... | 1984 | 6527374 |
| a preliminary serological survey of viral antibodies in peruvian sheep. | this study reports the sero-prevalence of viral infections in sheep in peru. serum samples were collected from 34 mature healthy rams located in 3 different geographic regions of the country (north, central and south). the sera were tested for antibodies to the following viruses: respiratory syncytial virus (rsv); parainfluenza 3 (pi-3) virus; bovine viral diarrhea/border disease (bvd/bd) virus; bovine herpesvirus 1 (bhv-1); bluetongue (bt) virus; ovine progressive pneumoniae (opp) virus; bovine ... | 1984 | 6528458 |
| early release of the density-dependent inhibition of phosphate uptake and atp synthesis after src gene expression in chick embryo fibroblasts. | our results showed that the expression of the src gene in chick embryo fibroblasts (cef) released the density-dependent inhibition (ddi) of phosphate metabolism (phosphate uptake and phosphorylation of small organic compounds). with increasing cell density, phosphate metabolism decreased by 58% in normal cef and, in contrast, increased by 20% in rous sarcoma virus (rsv)-transformed cef. the same change in the ddi was observed in cef infected by ny68 (a ts mutant for transformation of rsv) and ma ... | 1984 | 6609830 |
| giant cell pneumonia due to respiratory syncytial virus. occurrence in severe combined immunodeficiency syndrome. | a 6-month-old male infant with a severe combined immunodeficiency syndrome was hospitalized for progressive respiratory distress. examination during hospitalization disclosed widespread pulmonary infiltrates that did not respond to intensive therapy. the patient died eight days after admission. autopsy disclosed pneumocystis carinii pneumonia and widespread giant cell pneumonia. respiratory syncytial virus (rsv) was grown from a lung specimen obtained at autopsy. specific immunofluorescent stain ... | 1984 | 6611144 |
| antibody response to respiratory syncytial virus structural proteins in children with acute respiratory syncytial virus infection. | the purified respiratory syncytial virus (rsv), randall strain contained 10 polypeptides (72,000 molecular weight [72k], 66k, 48k, 42k, 40k, 36k, 30k, 23k, 18k, and 15k), 8 of which proved to be virus specific, and polypeptides 48k and 23k were glycosylated. in addition, a high-molecular-weight (150k), virus-specific glycopolypeptide was immunoprecipitated from rsv-infected cell lysate. the antibody response in human sera serially collected from children with primary rsv infection was mainly dir ... | 1985 | 3973971 |
| monoclonal capture antibody elisa for respiratory syncytial virus: detection of individual viral antigens and determination of monoclonal antibody specificities. | an enzyme-linked immunosorbent assay (elisa) for respiratory syncytial virus (rsv) that employs solid-phase monoclonal antibodies was developed. rsv antigens bound by these monoclonal capture antibodies were detected by addition of a polyclonal bovine antiserum, followed anti-bovine enzyme conjugate and enzyme substrate. the sensitivity and specific of the assay were determined by titrations of the solid-phase antibodies and by antigen titrations with both unpurified rsv-infected cell culture ma ... | 1985 | 3981003 |
| [experimental infection of lambs with strains of the bovine respiratory syncytial virus]. | lambs were successfully infected with bovine strains of a respiratory syncytial virus. when the nomi reference strain and the local antonovo strain of the rsv were used i/v and via the trachea the animals reacted on the 3rd to 5th day with dizziness, dyspnea, conjunctivitis, nasal discharges, etc., more strongly manifested with the use of the antonovo strain. the temperature reaction started on the 3rd day and receded on the 7th day, with a second peak on the 8h day. serologically, rsv-antibodie ... | 1985 | 3992914 |
| lobar collapse with respiratory syncytial virus pneumonitis. | in a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis. | 1985 | 4000751 |
| respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal response. | in order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ... | 1985 | 4000767 |
| severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. | respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ... | 1985 | 4000987 |
| quantitative aspects of passive immunity to respiratory syncytial virus infection in infant cotton rats. | the amount of passively acquired serum respiratory syncytial virus (rsv)-neutralizing antibodies required to protect the respiratory tract of cotton rats against infection was studied. infant cotton rats were inoculated intraperitoneally with various dilutions of a single pool of sera derived from cotton rats convalescent from rsv infection. after 24 h, these animals were inoculated with rsv intranasally. virus replication in the respiratory tract was suppressed in cotton rats which had a serum ... | 1985 | 4020957 |
| severe rsv bronchiolitis in an immunocompromised child. | a 9-year-old with immunodeficiency developed a severe, diffuse respiratory illness that necessitated mechanical ventilation. open lung biopsy revealed respiratory syncytial virus (rsv) as the sole pathogen. rsv detection should be included in the differential diagnosis of diffuse lung disease in an immunocompromised child. | 1985 | 4064461 |
| cytotoxic lymphocytes in the lungs of mice infected with respiratory syncytial virus. | mice infected with respiratory syncytial virus (rsv) developed cytotoxic lymphocytes in the lungs, which lysed rsv-infected, but not uninfected cells. cytotoxic activity was greatest 7 to 9 days after infection, was virus-specific, mhc-restricted and abolished by treatment of lymphocytes with anti-thy 1.2 or with anti-lyt 2.2 sera and complement. there was a close temporal relationship between the appearance of these cytotoxic lymphocytes in the lung and clearance of virus. in contrast, rsv pers ... | 1985 | 2866227 |
| isolation of a line of immortal chicken embryo fibroblasts after transfection with the nuclei of rous sarcoma virus-transformed chinese hamster cells. | secondary cultures of chicken embryo fibroblasts were transfected with purified nuclei from lysed cells of a clonal line of temperature-sensitive rous sarcoma virus (tsrsv)-transformed chinese hamster fibroblasts. after propagation for 3 months an established cell line designated chr32 was obtained in one chicken cell culture. the cells of this line have been propagated so far for 18 months, whereas normal chicken embryo fibroblasts died after 2 months. the established cells were heteroploid wit ... | 1985 | 2981172 |