Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| transcription of herpes simplex virus type 1 dna by eukaryotic and prokaryotic rna polymerases: size and sequence analysis of rna. | 1979 | 220787 | |
| isolation and localization of herpes simplex virus type 1 mrna abundant before viral dna synthesis. | herpes simplex virus type 1 (hsv-1) dna covalently bound to cellulose was used as a reagent to isolate viral rna transcripts for size analysis on denaturing agarose gels. nuclear and polyribosomal rna isolated at 2 h postinfection (p.i.) migrated with sizes between 1,500 and 5,500 nucleotides. at 6 h p.i. (when viral dna synthesis is underway), viral polyribosome-associated polyadenylated rna showed different discrete sizes of species predominating, with rna larger than 5,500 nucleotides clearly ... | 1979 | 225564 |
| control of herpes simplex virus type 1 mrna synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsk. | this paper deals with control of mrna levels, assayed by in vitro translation, in cells infected with herpes simplex virus type 1 (hsv-1). a particularly useful marker has been pyrimidine deoxyribonucleoside kinase (dpyk) mrna, for which the enzymatically active product can be assayed quantitatively. cells infected with the hsv-1 temperature-sensitive mutant tsk at the nonpermissive temperature (38.5 degrees c) or with wild-type hsv-1 in the continuous presence of cycloheximide contained no dete ... | 1979 | 219222 |
| phosphorylation of arabinofuranosylthymine in non-infected and herpesvirus (tk+ and tk-)-infected cells. | the phosphorylation of arabinofuranosylthymine (arathd) has been studied both in non-infected cells and in those infected with herpes simplex virus (hsv-1, lennette; hsv-1, ies and hsv-2, d-316). in these experiments, hsv strains were used which either contain (lennette, tk+ and d-316 tk+) or lack (ies, tk-) the capacity to induce pyrimidine deoxyribonucleoside kinase. it was found that extracellularly administered arathd is phosphorylated to ara ttp via aratmp and aratdp in both non-infected an ... | 1979 | 225422 |
| association of herpes simplex virus (hsv) with cervical cancer by lymphocyte reactivity with hsv-1 and hsv-2 antigens. | twenty-six women with invasive cervical cancer were examined for lymphocyte responsiveness to phytohemagglutinin (pha) herpes simplex virus (hsv). blood specimens were obtained from each patient before radiation therapy and separated into subpopulations by ficoll-hypaque centrifugation. lymphocytes were cultured in rpmi-1640 containing autologous plasma and exposed to pha, hsv-1, hsv-2, and control antigens. the results compared with those of women having negative papanicolaou smears and matched ... | 1979 | 223438 |
| transfection of human lymphoblastoid cells with herpes simplex viral dna. | the "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. one microgram of herpes simplex virus type 1 dna regularly initiated virus replication in four lymphoblastoid cell lines. per 10(5) cells exposed to 1 microgram of dna, 0.5--5 cells formed an infectious center. the minimal infective dose of dna was approximately 500 ng. | 1979 | 218224 |
| molecular genetics of herpes simplex virus. ii. mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells. | we have mapped the location in herpes simplex virus (hsv) dna of (i) three mutations at different loci (syn loci) which alter the social behavior of infected cells from clumping of rounded cells to polykaryocytosis, (ii) a mutation which determines the accumulation of one major glycoprotein [vp8.0(c(2))], and (iii) the sequences encoding four major virus glycoproteins [vp8.0(c(2)), vp7(b(2)), vp8.5(a), and vp19e(d(2))]. the experimental design and results were as follows. (i) analysis of hsv-1 x ... | 1979 | 219254 |
| anatomy of herpes simplex virus dna. xi. apparent clustering of functions effecting rapid inhibition of host dna and protein synthesis. | herpes simplex virus type 2 (hsv-2) strains inhibit the synthesis of both dna and protein of the host cell more rapidly than hsv-1 strains. several intertypic hsv-1 x hsv-2 recombinants and parental strains were examined for their ability to inhibit rapidly the synthesis of host protein and dna. the two functions cosegregated in all of eight recombinants tested and are therefore controlled by the same gene or by different genes in the same region of the viral dna. | 1979 | 219262 |
| [comparative study of the effectiveness of the purification and concentration of herpes simplex virus types 1 and 2]. | the results of comparative studies on concentration and purification of herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) by ficol density gradient centrifugation are presented. a two-phase distribution of extracellular hsv was established in phycol density gradient centrifugation: in zones with density of 1.110-1.114 and 1.088-1.085 g/ml. the effectiveness of purification of hsv preparations recovered from the corresponding gradient zones was determined by electron microscopy and quantit ... | 1979 | 219611 |
| persistence of herpes simplex virus types 1 and 2 in infected individuals. | during a 23-month period, 18 patients with facial or genital herpetic lesions were examined; culture specimens from each patient were obtained three times per week for virologic studies. the isolated viruses were identified, and the average duration of herpesvirus in lesions was determined. herpes simplex virus type 1 (hsv-1) was isolated from facial lesions for a mean duration of 3 1/2 days. in contrast, herpes simplex virus type 2 (hsv-2) was isolated from genital lesions for a mean duration o ... | 1979 | 219784 |
| evaluation of indirect hemagglutination and its inhibition in the differentiation between antibodies to herpes simplex virus types 1 and 2 for seroepidemiologic studies: use of a ii/i index threshold of 85 and an assay of type-specific antibodies. | two parameters, a ii/i index threshold value of 85 and an assay of type-specific antibodies to herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), were assessed, for seroepidemiologic purposes, for their efficacy in the establishment of past infections with hsv-1 and hsv-2 as indicated by the results of tests for indirect hemagglutination and its inhibition. neither criterion alone established past infection with either hsv-1 or hsv-2. however, by combination of the two parameters, antibodie ... | 1979 | 220339 |
| persistence, reactivation, and cell transformation by human herpeviruses: herpes simplex 1, 2 (hsv-1, hsv-2), cytomegalovirus (cmv), varicella-zoster (vzv), epstein-barr virus (ebv). | 1979 | 222414 | |
| thin-layer immunoassay for determination of antibodies to herpes simplex virus. | thin-layer immunoassay (tia) is a simple serological technique suitable for analysis of large numbers of samples. in this study, tia was evaluated for determination of antibodies to herpes simplex virus. herpes simplex virus antigen used in tia was purified from material released from virus-infected cells. the results obtained by tia were compared with those obtained by neutralization and complement fixation tests. tia was found to be as sensitive as the neutralization test for demonstration of ... | 1979 | 222798 |
| herpes virus infection as a cofactor in carcinogenesis: supernatants of herpes virus type 1- and type 2-infected cell cultures contain a cell growth-stimulating factor. | some cell cultures synthesize a mitogenic factor (dnasf) following their lytic infection with either hsv-1 or hsv-2 which is shed into the medium. of six cell lines permissive to hsv infection tested, three produce dnasf (cv1, hf, mca) and a significantly higher (3h)-tdr incorporation was obtained with indicator cells growing in supernatants of virus-infected cells (supv+) than in the corresponding supernatants of sham-treated cells (supv-). sometimes the values obtained with supv+ exceed those ... | 1979 | 223098 |
| persistence of herpes simplex virus type 1 in rat neurotumor cells. | herpes simplex virus type 1 (hsv-1) infection of a rat central nervous system tumor cell line led to almost complete destruction of the cells. cells that survived the infection could be isolated and shown to produce infectious hsv particles for variable lengths of time in culture ranging from 20 to 57 passages. even though infectious virus production eventually ceased, the cell lines continued to produce herpes-specified proteins as measured by immunological techniques. these cells also showed h ... | 1979 | 219234 |
| herpes simplex virus types 1 and 2 in organotypic cultures of mouse central and peripheral nervous system. 2. electron microscopic observations of myelin degeneration. | organotypic cultures of mouse spinal cord with attached dorsal root ganglia, which contain both central and peripheral myelin in the one unit of tissue, were infected with hsv 1 or hsv 2 and studied using electron microscopy. intranuclear viral nucleocapsids and intracytoplasmic enveloped particles were found in the schwann cells associated with peripheral myelin and in oligodendroglia associated with central myelin. degeneration of peripheral myelin most commonly involved an asymmetrical swelli ... | 1979 | 219153 |
| lethal herpes simplex virus type 1 hepatitis in a normal adult. | a fatal case of human herpes simplex type 1 hepatitis is presented and compared with cases previously reported. the patient presented with fulminant hepatic necrosis, coagulopathy, leukopenia, thrombocytopenia, and absence of typical herpetic mucocutaneous lesions. liver biopsy helped to establish a presumptive diagnosis in this patient and in one previously reported case. theoretically, early diagnosis might enable application of specific antiviral therapy in future cases of this usually fatal ... | 1979 | 218866 |
| reduction of 51cr-permeability of tissue culture cells by infection with herpes simplex virus type 1. | infection of different strains of tissue culture cells with herpes simplex virus type 1 (hsv-1) resulted in a reduced 51cr-permeability. a stability of the cellular membrane to triton x-100, toxic sera and hsv-specific complement-mediated immune-cytolysis could be observed simultaneously. the results differed with respect to the cell strain used in the experiments. | 1979 | 218898 |
| nucleosides. 110. synthesis and antiherpes virus activity of some 2'-fluoro-2'-deoxyarabinofuranosylpyrimidine nucleosides. | a series of 5-substituted 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)cytosines 7a-d and their corresponding uracils 9a-d,f were prepared by condensation of 3-o-acetyl-5-o-benzoyl-2-deoxy-2-fluoro-d-arabinosyl bromide (5) with appropriately trimethylsilylated pyrimidines followed by saponification of the protected nucleosides 6 or 8. 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (7e) was obtained by iodination of 7a. iodination of 8a followed by removal of the protecting acyl-prote ... | 1979 | 218006 |
| [detection of antibodies to herpes simplex virus type 1 and cytomegalovirus by an immunoenzyme technic. comparison with complement fixation]. | 1980 | 6100770 | |
| new model cell systems (pk and xtc-2) for studying acute and persistent infections with herpes simplex and pseudorabies viruses. | herpes simplex virus type 1 (hsv-1) showed limited replication in pk (pig kidney) and xtc-2 (xenopus laevis frog) cell lines. virus replication depended on the multiplicity of infection (moi). at a high moi, hsv-1 caused a typical cytopathic effect (cpe) in xtc-2 cells but a little marked cpe in pk cells. pseudorabies virus (prv) replicated intensively in pk cells (permissive system) but not in xtc-2 cells (nonpermissive system). both viruses were adsorbed on to pk and xtc-2 cells. in infected p ... | 1980 | 6106375 |
| detection of herpesvirus antigens by solid-phase radioimmunoassay with 125i-antiglobulin and 125i-protein a. | the binding of immune (is) and non-immune (ns) sera to herpes simplex virus type 1- (hsv-1) infected vero cells was tested by indirect solid-phase radioimmunoassay (ria) with radioiodinated swine anti-rabbit igg (125i-swar-igg) or staphylococcal protein a (125i-spa). to indicate the binding of non-immune igg molecules to virus-induced fc-receptors, the cells were incubated in the presence or absence of the glycosylation inhibitor 2-deoxy-d-glucose (dog). with 125i-swar-igg, the binding of both i ... | 1980 | 6106377 |
| preparation of immune serum to immediate early and early polypeptides specified by herpes simplex virus type 1. | an antiserum to the immediate early (ie) ana early (e) polypeptides specified in sirc cells within 4 hr post infection (p.i.) with herpes simplex virus type 1 (hsv1) was prepared in rabbits by sequential injections of two different antigens. the first antigen, which contained the alpha polypeptides, was prepared from infected cells treated for 5 hr with cycloheximide and incubated in the presence of actinomycin d. the second antigen contained all polypeptides, synthesized for 4 hr after removal ... | 1980 | 6107018 |
| restriction of latent herpes virus infection in rabbits immunized with subviral herpes simplex virus vaccine. | the ability of an experimental herpes simplex virus type 1 (hsv) vaccine to influence the establishment of latent infection was examined. the virion-free vaccine was prepared from hsv 1-infected lep cells by extraction with nonidet p 40. albino rabbits were immunized with three doses of the subviral vaccine (strain kos) and challenged with 10(5) pfu of hsv 1 (kupka strain) into the right scarified cornea. non-immune controls and rabbits immunized with formalin-inactivated virion vaccine (strain ... | 1980 | 6107035 |
| raised cytomegalovirus-antibody level in cerebrospinal fluid of schizophrenic patients. | the serum and cerebrospinal fluid (csf) of 60 schizophrenic patients and 26 controls were analysed for viral antibody against cytomegalovirus (cmv), vaccinia virus, herpes simplex virus type 1 (hsv), and type a influenza virus. a csf/serum antibody ratio more than 2 standard deviations above the mean of the controls suggested local antibody production in the central nervous system. 68% of the patients had an increased csf/serum antibody ratio for cmv antibody, 14% for vaccinia antibody, 4% for h ... | 1980 | 6107453 |
| immunogenicity of subviral herpes simplex virus preparations: protection of mice against intraperitoneal infection with live virus. | the capability of crude antigen extracts from herpes simplex virus type 1-infected human diploid cells (am) to induce protection against intraperitoneal challenge with homotypic but heterologous virus in mice was investigated. the administration of repeated doses of am without adjuvant failed to confer significant protection. but one relatively small dose of am with complete freund's adjuvant resulted in a protective effect. a good correlation was observed between the protective activity of am p ... | 1980 | 6111199 |
| correlation of the chemical structure of 4-nitroquinolines inactivating human cytomegalovirus and established in vivo carcinogenicity tests. | inactivation of the infectivity of human cytomegalovirus (cmv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) has been observed following exposure to 4-nitroquinoline 1-oxide (nqo) or its metabolite, 4-hydroxyaminoquinoline 1-oxide (haqo). the present study of the specificity of the chemical structure of 4-nitroquinolines demonstrated that both the 4-nitro and 1-oxide groups were required for inactivation of virus infectivity. reduction of the 4-nitro group to a 4-hydroxyamino group ... | 1980 | 6119808 |
| biochemical transformation of mouse cells by a purified fragment of marmoset herpesvirus dna. | although the size of marmoset herpesvirus (marhv) dna, estimated by velocity sedimentation in sucrose gradients, was similar to that of herpes simplex virus type 1 (hsv-1) dna, the restriction endonuclease sites of marhv and hsv-1 dnas were quite different. a specific bamhi restriction fragment (6.2 x 10(6) daltons) of marhv dna biochemically transformed lm(tk-) mouse fibroblasts to the thymidine kinase(tk)-positive phenotype. rabbit antisera, prepared against marhv tk, inhibited marhv-induced t ... | 1980 | 6154671 |
| structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2. | capsids of herpes simplex virus (hsv) types 1 and 2 contain seven polypeptides ranging in molecular weight from 154,000 to 12,000 (termed nc-1 through nc-7 in order of descending molecular weight). antibodies prepared to hsv-1 capsid polypeptides isolated from sodium dodecyl sulfate-polyacrylamide gels reacted in an immunofluorescence assay against hsv-1-infected kb cells. three of the antibodies (anti-nc-1, anti-nc-2, and anti-nc-3,4) also reacted with hsv-2-infected cells. tryptic peptide anal ... | 1980 | 6154808 |
| macrophage dependence of polyriboinosinic acid-polyribocytidylic acid-induced resistance to herpes simplex virus infection in mice. | the relative contributions of macrophages and lymphocytes to the induction of resistance to primary herpes simplex virus type 1 (hsv-1) infection by polyriboinosinic-polyribocytidylic acid complex [poly (i:c)] were investigated in c58 mice. the induction of resistance was found to be strongly dependent on macrophages compared to lymphocytes. macrophage-deficient (silica-treated) mice produced less interferon and were not as responsive to prophylactic treatment of hsv-1 infections with poly (i:c) ... | 1980 | 6155345 |
| enhanced replication of herpes simplex virus type 1 in bhk-21 cells transformed by hamster sarcoma virus. | untransformed and hamster sarcoma virus-transformed bhk-21 cells were compared for their ability to support productive infection with herpes simplex virus type 1. in both types of cells, infection with herpes simplex virus resulted in a lytic, productive cycle. however, inhibition of host cell rna and dna synthesis, transcription and replication of viral dna, and production of infectious virus began earlier and reached higher levels in transformed cells. | 1980 | 6162823 |
| herpes simplex virus antigens and antibodies: a survey of studies based on quantitative immunoelectrophoresis. | herpes simplex virus type 1 and type 2 antigens extracted from infected cells with use of nonionic detergent produce a number of distinct immunoprecipitates in crossed immunoelectrophoresis (cie) with hyperimmune rabbit antibodies to herpes simplex virus. the analytic power of cie combined with the use of single immunoprecipitates made it possible, for a number of individual antigens, to determine (1) the immunogenicity of the antigens in experimentally infected animals and naturally infected hu ... | 1980 | 6164087 |
| interferon production on mouse fibroblasts induced by various viruses. | the interferon (if) inducing ability of some dna and rna viruses on mouse ccl-1 cells was investigated. the f and h strains of newcastle disease virus (ndv) and sendai virus proved to be the best inducers. gd-7, mengo and vesicular stomatitis virus (vsv) were middle inducers, herpes simplex 1 (hsv 1) was a weak inducer, and adenovirus 12 and semliki forest (sfv) were non-inducers. moreover antiviral resistance induced by the same viruses and if production generally followed parallel paths. | 1980 | 6161620 |
| deoxyribopyrimidine triphosphatase activity specific for cells infected with herpes simplex virus type 1. | nuclei from baby hamster kidney cells infected with herpes simplex virus type 1 contain a virus-specific deoxyribonucleoside triphosphate degrading activity. the reaction proceeds at 4 degrees c and can thus be distinguished from host enzymes. under these conditions the enzyme is specific for deoxyribopyrimide triphosphates and catalyzes pyrophosphate cleavage to produce the monophosphates, dutp being the best substrate followed by dctp and dttp. the appearance of the activity after infection pa ... | 1980 | 6103541 |
| characterization of herpes simplex virus strains isolated from patients with various diseases. | herpes simplex virus strains (hsv) were isolated from various herpetic diseases. these hsv-strains isolated in bulgaria (687) and in other countries (13) were studied by the neutralization test using standard type specific rabbit antisera and human gamma globulin. the serotype distribution of all strains showed: hsv-1 = 323 strains, hsv-2 = 372 strains, 5 strains behaved antigenically intermediate. a close correlation between the serotype of the strains and the localization of the lesions was es ... | 1980 | 6243918 |
| different susceptibilities of skin to type 1 and type 2 herpes simplex viruses in newborn rabbits. | skin infections with type 1 herpes simplex virus (hsv-1) were compared with skin infections with type 2 virus (hsv-2). five strains each of hsv-1 and hsv-2 were tested by injecting 10(3) 50% tissue culture infective doses of each strain subcutaneously into 1-day-old new zealand white rabbits. all five strains of hsv- 2 produced severe skin lesions that resulted in wide dissemination of the infection to many organs, paralysis of the hind legs, and finally death. the virus could be isolated freque ... | 1980 | 6244227 |
| acycloguanosine treatment of herpesvirus infections in footpads and nervous tissue of normal and immunosuppressed mice. | treatment of herpes simplex virus type 1 (hsv-1)-infected mice with acycloguanosine resulted in significantly fewer virus isolates from nervous tissues than from tissues of untreated animals. acycloguanosine also significantly reduced the mean titers of hsv-1 recovered from the footpads of drug-treated animals. the anti-herpetic activity of acycloguanosine was observed in both normal and immunosuppressed mice. | 1980 | 6244239 |
| distribution of sequences homologous to the dna of herpes simplex virus, types 1 and 2, in the genome of pseudorabies virus. | the distribution of related sequences between the genomes of pseudorabies virus (prv) and herpes simplex virus, types 1 (hsv-1) and 2 (hsv-2), was determined. approximately 7% of the sequences in prv are shared by hsv-1 and hsv-2 dnas. by means of the southern blot technique, it was found that the homologous sequences are not sequestered in one region but are distributed throughout the prv genome. hsv-1 and hsv-2 have the greatest homology with the long unique region of prv dna and the least wit ... | 1980 | 6244240 |
| a type-specific antiserum induced bya major herpesvirus type 1 glycoprotein. | a type-specific antiserum was prepared against vp 7/8, the major hsv-1 type-specific glycoprotein. the specificity of this antiserum was demonstrated by both neutralization of hsv-1 infectivity and immunoprecipitation followed by sds-polyacrylamide gel electrophoresis of the immunoprecipitates. only minimal cross-reactivity with hsv-2 was observed. this major vp 7/8 glycorprotein was also purified from a host of virus-induced proteins with the specific immunoadsorbent prepared with anti-vp 7/8 s ... | 1980 | 6244352 |
| synthesis and biological activities of 5-(hydroxymethyl, azidomethyl, or aminomethyl)-2'-deoxyuridine and related 5'-substituted analogues. | the synthesis of 5-(azidomethyl)-2'-deoxyuridine (10) has been accomplished by two independent methods. the first involved tosylation of 5-(hydroxymethyl)-2'-deoxyuridine (1) to furnish a mixture of two mono- and a ditosyl nucleosides which were converted into the corresponding 5-(azidomethyl) (10), 5-(azidomethyl)-5'-azido (14), and 5-(hydroxymethyl)-5'-azido (15) derivatives of 2'-deoxyuridine. the second method was more selective and required the formation of the intermediate 5-(bromomethyl)- ... | 1980 | 6244411 |
| damage and repair in mammalian cells after exposure to non-ionizing radiations. ii. photoreactivation and killing of rat kangaroo cells (potorous tridactylus) and herpes simplex virus-1 by exposure to fluorescent "white" light or sunlight. | photoreactivation (pr) of ultraviolet (254 nm)-inactivated cornea cells of the potoroo (or rat kangaroo; potorous tridacylus) has been studied at wavelengths greater than 375 nm from either fluorescent "white" light or sunlight. in both cases the pr kinetics curves pass through maxima, which most likely result from the superposition of concomitant inactivation by the photoreactivating light. the inactivating effect of light was directly demonstrated for non-uv-irradiated cells, permitting correc ... | 1980 | 6244485 |
| cloning of the herpes simplex virus type 1 thymidine kinase gene in e. coli k 12: a selective marker for gene transfer into animal cells. | the herpes simplex virus type 1 (hsv 1) thymidine kinase (tk) gene, carried by a 3.6 kb bam h1 dna fragment, was isolated and ligated to bam h1 cleaved bacterial plasmid pbr 322. the tk gene, cloned in e. coli, is functional when it is transferred back into eukaryotic cells, and can thus be used as a selective marker for gene transfer. | 1980 | 6245004 |
| induction of cytotoxic t lymphocytes against herpes simplex virus type i: role of accessory cells and amplifying factor. | spleen cells from mice primed with herpes simplex virus type 1 (hsv-1) could be induced to differentiate into cytotoxic t lymphocytes (ctl) by in vitro culture with infectious hsv-1 but not by heat-inactivated virus. induction of ctl failed to occur if the spleen cells were depleted of adherent cells by passage over columns of nylon wool before culture with virus. the ctl response could be restored by adding normal syngeneic peritoneal cells (pc) or l cell fibroblasts but not by allogeneic pc or ... | 1980 | 6245140 |
| some characteristics of an early protein (icp 22) synthesized in cells infected with herpes simplex virus. | in vero cells incubated at 40 degrees c or treated with azetidine at 37 degrees c, synthesis of a polypeptide ('c') of apparent mol. wt. 66000 was stimulated. it was not phosphorylated and was found in the cytoplasmic fraction of cell lysates. in cells infected with herpes simplex virus type 1 (hsv-1) in the presence of azetidine, synthesis of cellular proteins, including polypeptide c, was suppressed and infected cell polypeptides icp 4, 0, 22 and 27 (apparent mol. wt. 170000, 120000, 75000 and ... | 1980 | 6245173 |
| synthesis of 5-[(methylthio)methyl]-2'-deoxyuridine, the corresponding sulfoxide and sulfone, and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition. | substitution on the alpha position of thymidine with methylthio (3) and methylsulfonyl (5) groups gave antiviral agents that were specific and relatively nontoxic inhibitors of herpes simplex virus replication in cell culture. the thioether (3) was effective against both types 1 and 2 of herpes simplex virus, whereas the activity of the sulfone derivative (5) was restricted to herpes simplex virus type 1. the sulfoxide derivative 1-(2-deoxy-beta-d-ribofuranosyl)-alpha-(methylsulfinyl)thymine (4) ... | 1980 | 6245212 |
| identification of the herpes simplex virus dna sequences present in six herpes simplex virus thymidine kinase-transformed mouse cell lines. | we have used a novel filter hybridization approach to detect and map the herpes simplex virus (hsv) dna sequences which are present in four hsv thymidine kinase (hsvtk+)-transformed cell lines which were derived by exposure of thymidine kinase negative (tk-) mouse cells to uv light-irradiated hsv type 2 (hsv-2). in addition, we have mapped the hsv-1 dna sequences which are present in two hsv-1tk+-transformed cell lines produced by transfection of tk- mouse cells with sheared hsv-1 dna. the resul ... | 1980 | 6245232 |
| characterization of rna synthesized in isolated nuclei of herpes simplex virus type 1-infected kb cells. | nuclei isolated from herpes simplex virus (hsv)-infected kb cells actively synthesize hsv rna in vitro; the rna can be hybridized with hsv dna or nuclear rna from hsv-infected cells. nascent rna molecules labeled in vivo with 32po4 were elongated, utilizing the nuclear system to incorporate hg-ctp at their 3' ends, and then isolated on an affinity column. hybridization of isolated nascent rna molecules showed that greater than 50% of them were hsv specific and that more than 25% were self-comple ... | 1980 | 6245253 |
| inhibition of herpes simplex virus type 1 specific translation in cells treated with poly(ri).poly(rc). | 1980 | 6154127 | |
| location and cloning of the herpes simplex virus type 2 thymidine kinase gene. | the herpes simplex virus type 2 thymidine kinase gene has been mapped to a position colinear with the herpes simplex virus type 1 thymidine kinase gene and cloned within a 4.0-kilobase fragment in pbr 322. | 1980 | 6245273 |
| activation of mouse retrovirus by herpes simplex virus type 1 cloned dna fragments. | 1980 | 6245529 | |
| human peripheral null lymphocytes. ii. producers of type-1 interferon upon stimulation with tumor cells, herpes simplex virus and corynebacterium parvum. | human blood lymphocytes, exposed for 6 to 24 h in vitro to tumor cells (k 562, igr3, l1210), herpes simplex virus type 1 (hsv) or corynebacterium parvum (cp), produced high levels of anti-viral activity which was identified as type-1 interferon (if). in mixed lymphocyte tumor cell cultures (mltc), the generated type-1 if was definitely shown to originate from the lymphocytes and not from the tumor cells. supplementation of leukocyte cultures with 10% fetal calf serum instead 10% human ab serum h ... | 1980 | 6157543 |
| comparison of foldback sequences of herpes simplex virus types 1 and 2 dna. | the dnas of herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) were separately denatured and allowed to renature briefly. the intrastand foldback structures that resulted from base pairing of inverted repeated sequences on otherwise single-stranded (ss) dna were visualized in the electron microscope. the two genomes were found to contain similar size classes of small duplex stem dna sequences. however, hsv-2 dna appeared to possess an additional, larger size class of foldback structures not fo ... | 1980 | 6243346 |
| physical mapping of herpes simplex virus type 1 ts mutants by marker rescue: correlation of the physical and genetic maps. | 1980 | 6243429 | |
| ga and gb glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide. | utilizing a combination of preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and sodium dodecyl sulfate-hydroxylapatite column chromatography, we have separated and purified the ga and gb glycoproteins of the major virus-specific glycoprotein region from herpes simplex virus type 1-infected cells. by using purified antigen preparations, antisera specific to each of these glycoproteins were produced. immunoprecipitation from detergent extracts of infected cells and radioimmune ... | 1980 | 6162034 |
| herpes simplex virus glycoproteins: isolation of mutants resistant to immune cytolysis. | immune cytolysis mediated by antibody and complement is directed against components of the major herpes simplex virus (hsv) glycoprotein complex (molecular weight, 115,000 to 130,000), comprised of ga, gb, and gc, and against glycoprotein gd-all present on the surfaces of infected cells. tests with a temperature-sensitive (ts) mutant of hsv-1 (tsa1) defective in glycoprotein synthesis at the nonpermissive temperature (39 degrees c) demonstrated that over 90% of mutant-infected cells maintained a ... | 1980 | 6246268 |
| isolation of the chicken thymidine kinase gene by plasmid rescue. | we have used the bacterial plasmid pbr322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. in this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase ecori/hindiii insert in br322. the cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene. | 1980 | 6246445 |
| a herpes simplex virus type 1 function continuously required for early and late virus rna synthesis. | 1980 | 6246451 | |
| two distinct loci confer resistance to acycloguanosine in herpes simplex virus type 1. | two distinct loci that confer resistance to acycloguanosine (acyclo-guo) in herpes simplex virus types 1 have been identified. the first locus is the gene for the virus-specific thymidine kinase (tk). mutations that decrease tk activity also render the virus resistant to acyclo-guo, and the level of resistance corresponds to the decrease in tk activity. acyclo-guo resistance due to defective tk expression is recessive to the wild-type phenotype, acyclo-guo-sensitive (acgs). we term this locus ac ... | 1980 | 6246531 |
| resistance of herpes simplex virus to acycloguanosine: role of viral thymidine kinase and dna polymerase loci. | acycloguanosine [9-(2-hydroxyethoxymethyl)guanine; acyclo-guo] is a potent inhibitor of herpes simplex viruses (hsv); it is selectively phosphorylated in virus-infected cells. in order to define those viral functions that may mediate resistance to acyclo-guo, the drug sensitivities of temperature-sensitive (ts) and phosphonoacetic acetic acid (paa)-resistant mutants of hsv-1 and hsv-2 have been determined. two distinct viral genetic loci are independently associated with acyclo-guo resistance. m ... | 1980 | 6246532 |
| comparative analysis of the virion polypeptides specified by herpes simplex virus type 2 strains. | this paper reports on the variability of structural polypeptides of 32 strains of herpes simplex virus 2 (hsv-2) isolated from various locations in the united states and italy. most strains were passaged a limited number of times at low multiplicity outside the human host; a few strains were characterized by numerous passages at variable multiplicities in cell culture. the acrylamide gel electrophoresis of polypeptides from purified virions revealed very few differences. considering these differ ... | 1980 | 6246854 |
| virus-specific basic phosphoproteins associated with herpes simplex virus type a (hsv-1) particles and the chromatin of hsv-1-infected cells. | herpes simplex type 1 angelotti (hsv-1 ang) virions were shown to contain two major acid-soluble proteins, bp1 and 2, which by size and charge analysis were also found to be associated with chromatin isolated from hsv-1 ang-infected african green monkey kidney cells (hsv-chromatin). bp1 and 2 proved to exist in a phosphorylated state both in virions and in hsv-chromatin. bp1 consisted of a single polypeptide of 38 k mol. wt. which was correlated to the tegument protein vp22. in sds-polyacrylamid ... | 1980 | 6247428 |
| comparative neurovirulence and latency of hsv1 and hsv2 following footpad inoculation in mice. | the effect of virus dose and animal age on the appearance of acute and latent neurologic infection by hsv1 and hsv2 was studied in balb/c and icr mice inoculated in the footpad. at low viral doses hsv2 was found to be 1,500 times more neurovirulent than hsv1. at high doses there was no difference in neurovirulence. age-acquired resistance to disease was shown to be less complete with hsv2 than with hsv1. neurovirulence was shown to be associated with spread of infection to the spinal ganglia. th ... | 1980 | 6247441 |
| unimpaired histone synthesis in human fibroblasts infected by human cytomegalovirus. | serum-starved human foreskin fibroblasts were infected by human cytomegalovirus (towne strain) that is thought to induce dna replication in host cells during lytic infection. at various times postinfection, the cultures were pulse labeled with either 3h-thymidine or 14c-thymidine and 3h-lysine to examine dna synthesis and histone synthesis, respectively. isopycnic centrifugation of labeled dna in cscl revealed that precursor incorporation into host-cell dna was enhanced over the control around 2 ... | 1980 | 6247630 |
| genetic retrieval of viral genome sequences from herpes simplex virus transformed cells. | oncogenic transformation of cultured cells by inactivated herpes simplex virus (hsv) types 1 and 2 has been demonstrated. expression of hsv information in these transformed cells has been shown by immunofluorescence studies, detection of hsv neutralizing antibody in sera from tumour-bearing animals and by hybridization of hsv-specific rna. molecular hybridization studies of dna from hsv-2 transformed hamster cells have detected up to 40% of the hsv genome present in several copies. complementati ... | 1980 | 6247659 |
| the treatment of herpetic whitlow--a new surgical concept. | characteristics of cutaneous lesions caused by herpes simplex virus (hsv-1) are: acquired by skin-to-skin contact; humans are the only natural host. often on the hands of health care personnel. painful swelling, erythema, vesicles, and ulcerations. possible involvement of cutaneous digital areas of paronychium, eponychium, and subungual matrix. similar to a bacterial (septic) or fungal felon. self-limiting--14- to 21-day course. an aseptic felon, which provides a contraindication to surgical inc ... | 1980 | 6247731 |
| alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzyme. | 1980 | 6247825 | |
| in vitro and in vivo resistance of herpes simplex virus to 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine). | in vitro passage of the patton strain of herpes simplex virus type 1 (hsv-1-p) in the presence of acycloguanosine (acg) led to the emergence of a highly drug-resistant strain (hsv-1-p-acg-r). over 1% of virions in 6 of 15 hsv strains tested were able to form plaques in the presence of 10 mumol of acg on first exposure to the drug. therefore, there exists among hsv strains a broad range of susceptibilities to acg, and some strains contain particles which are partially resistant to acg before ever ... | 1980 | 6247967 |
| oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition. | 5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ... | 1980 | 6248643 |
| combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2. | the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ... | 1980 | 6249191 |
| 2'-fluoro-5-iodo-aracytosine, a potent and selective anti-herpesvirus agent. | a newly synthesized pyrimidine analog, 2'-fluoro-5-iodo-aracytosine (fiac), suppressed by 90% the replication of various strains of herpes simplex virus types 1 and 2 at concentrations of 0.0025 to 0.0126 microm. cytotoxicity was minimal, as determined by trypan blue dye exclusion with norman vero, wi-38, and nc-37 cell proliferation; the 50% inhibitory dose was 4 to 10 microm in a 4-day assay. when compared with other antiviral drugs, fiac was active at much lower concentrations than arabinosyl ... | 1980 | 6249196 |
| marrow-dependent cells depleted by 89sr mediate genetic resistance to herpes simplex virus type 1 infection in mice. | adult mice resistant to infection with 10(6) plaque-forming units of a virulent strain of herpes simplex virus type 1 were treated with 89sr to abrogate marrow-dependent cell functions. treated mice were found to be much more susceptible to the herpes simplex virus type 1 infection than untreated mice. the virus persisted in the visceral tissues of 89sr-treated mice for 3 or more days postinfection but not in those of untreated mice. the virus also spread to the spinal cords of treated but not u ... | 1980 | 6249746 |
| differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells. | fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ... | 1980 | 6249749 |
| ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2. | the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ... | 1980 | 6249764 |
| herpes simplex virus thymidine kinase transcripts are absent from both nucleus and cytoplasm during infection in the presence of cycloheximide. | nick-translated dna from a recombinant plasmid containing the herpes simplex virus type 1 thymidine kinase gene was used as a probe for the synthesis of thymidine kinase rna. the recombinant plasmid was generated by inserting the 3.5-kilobase fragment derived by bamhi digestion of herpes simplex virus type 1 dna into plasmid pbr322. at 8 h after infection, cytoplasmic and nuclear rna hybridized to 14% and 19% of the recombinant dna probe, respectively. however, no significant hybridization was f ... | 1980 | 6253662 |
| viral dna synthesis is required for the efficient expression of specific herpes simplex virus type 1 mrna species. | 1980 | 6243816 | |
| the incorporation of 5-iodo-5'-amino-2',5-dideoxyuridine and 5-iodo-2'-deoxyuridine into herpes simplex virus dna. relationship between antiviral activity and effects on dna structure. | isopycnic centrifugation in cscl gradients was used to quantify the incorporation of 5-iodo-5'-amino-2',5'-dideoxyuridine and 5-iodo-2'-deoxyuridine into herpes simplex virus type 1 dna. a parallelism between the degree of incorporation into viral dna and the inhibition of herpes simplex virus type i replication was found for both thymidine analogs. a concentration of 5-iodo-5'-amino-2',5'-dideoxyuridine approximately 100 times greater than 5-iodo-2'-deoxyuridine was required to achieve similar ... | 1980 | 6243982 |
| serum antibodies to herpes simplex virus type 1 during active oral herpes infection. | subjects with oral herpes lesions at the time of serum sampling had higher-efficiency antibody (higher proportion of neutralizing antibody as determined by plaque reduction, compared with total antibody as detected by radioimmunoassay) to herpes simplex virus type 1 (hsv-1) than did subjects with no lesions at the time of serum sampling. these higher-efficiency sera also had higher antibody titers to structural components of herpes simplex virus type 1 than did the low-efficiency sera. absorptio ... | 1980 | 6244224 |
| cell-mediated immunity against herpes simplex induction of cytotoxic t lymphocytes. | the conditions required for the induction of both primary cytotoxic t lymphocytes (ctl) in vivo and secondary ctl in vitro against herpes simplex virus type 1 (hsv-1)-infected cells were defined. primary ctl responses occurred only in mice exposed to infectious hsv-1. these responses, which were shown to be mediated by t lymphocytes, peaked at 1 week and had disappeared by 2 weeks after infection. the level of primary cytotoxicity was enhanced by treatment of mice with cyclophosphamide before in ... | 1980 | 6244225 |
| herpes simplex virus phosphoproteins. i. phosphate cycles on and off some viral polypeptides and can alter their affinity for dna. | we report on phosphorylation, the stability of the bound phosphate, and the properties of several phosphorylated infected-cell polypeptides (icps) synthesized in cells infected with herpes simplex virus 1 and 2. our results and conclusions are as follows. (i) phosphorylation of icps occurs by at least two different pathways. thus, the 4a and 4c electrophoretic forms of icp 4 were labeled with 32p during a pulse concurrently with their synthesis, whereas icp 22 and icp 27 were labeled with 32p o ... | 1980 | 6245226 |
| ultrastructural characterization of an early, nonstructural polypeptide of herpes simplex virus type 1. | an immunoperoxidase procedure was employed to study the expression of a large-molecular-weight, virus-induced polypeptide (vp175; molecular weight, 175,000) at the light and electron microscopic levels in vero cells infected with herpes simplex virus type 1 or with tsb2, a dna-negative, temperature-sensitive mutant of herpes simplex virus type 1. in cells infected with herpes simplex virus type 1 and in cells infected with tsb2 at the permissive temperature (34 degrees c), vp175 was found within ... | 1980 | 6245269 |
| comparison of indirect hemagglutination and 51chromium release tests for detection of herpes simplex virus type 1 and 2 antibodies in patients with recurrent herpes infections. | indirect hemagglutination and 51cr release tests (ihat and 51-crt respectively) were compared in patients with recurrent herpes simplex virus (hsv) infections from whom hsv-1 or hsv-2 was isolated. both tests were equally sensitive and specific in detecting hsv antibodies. however, ihat was more specific in detecting homologous hsv antibody response in patients with recurrent hsv-2 infections. past infections with hsv-1 in the patients with dual infections were detected by determining hsv-type s ... | 1980 | 6254476 |
| reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls. | serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ... | 1980 | 6254649 |
| gene expression of herpes simplex virus. ii. uv radiological analysis of viral transcription units. | the transcriptional organization of the genome of herpes simplex virus type 1 was analyzed by measuring the sensitivity of viral polypeptide synthesis to uv irradiation of the infecting virus. herpes simplex virus type 1 was irradiated with various doses of uv light and used to infect xeroderma pigmentosum fibroblasts. immediate early transcription units were analyzed by having cycloheximide present throughout the period of infection, removing the drug at 8 h postinfection, and pulse-labeling pr ... | 1980 | 6247499 |
| the effect of polyamines on herpes simplex virus type 1 dna polymerase purified from infected baby hamster kidney cells (bhk-21/c13). | the dna polymerase whose synthesis is directed by the herpes simplex virus type 1 (hsv-1) dna was purified 545-fold from bhk-21/c13 cells 16 h after infection with the virus. spermidine and spermine stimulated the activity of the polymerase over the concentration range 0.5 mm to 2.5 mm. this effect was enhanced with increased concentrations of polyamine, maximum stimulation being threefold and fourfold for spermidine and spermine respectively. the diamine, putrescine, had little effect on the en ... | 1980 | 6255073 |
| the effect of delta-9-tetrahydrocannabinol on herpes simplex virus replication. | both herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) failed, in an identical fashion to replicate and produce extensive c.p.e. in human cell monolayer cultures which were exposed (8 h before infection, at infection, or 8 h p.i.) to various concentrations of delta-9-tetrahydrocannabinol. similar results were obtained with a plaque assay utilizing confluent monkey cells. possible mechanisms for this antiviral activity are discussed. | 1980 | 6255077 |
| quantification of plasminogen activator activity associated with herpesvirus-transformed cells. | herpesvirus-transformed cell lines were examined for plasminogen activator (pa) activity using a quantitative assay. previous results of cold fibrin overlay assays indicated that herpesvirus-transformed hamster cell lines produce fibrinolytic activity. quantification of this activity involved the use of an 125i-fibrin lysis assay in which medium previously incubated with transformed or normal cells was tested for its ability to lyse 125i-fibrin polymers. this assay indicated an enhanced producti ... | 1980 | 6255081 |
| cell-mediated immunity in herpes simplex virus-infected mice: suppression of delayed hypersensitivity by an antigen-specific b lymphocyte. | lymph node cells, regional to the site of infection with herpes simplex virus type 1 (hsv-1), when taken 1 to 9 months p.i. and transferred to hsv-1 immune recipients, suppressed the delayed type hypersensitivity (dth) reaction to the virus. the suppressive activity was specific for hsv-1 and was transferred by a thy-1.2-negative, ig-positive, lymphocyte population. anti-hsv-1 serum did not suppress the hsv-1-induced dth response. contralateral lymph nodes contained little or no suppressor cell ... | 1980 | 6249886 |
| comparative structural analysis of glycoprotein gd of herpes simplex virus types 1 and 2. | we studied the synthesis and processing of the type-common glycoprotein gd in herpes simplex virus type 2 (hsv-2) and compared it structurally to glycoprotein gd of herpes simplex virus type 1 (hsv-1). we demonstrated that in hsv-2, gd undergoes posttranslational processing from a lower-molecular-weight precursor (pgd51) to a higher-molecular-weight product (gd56). tryptic peptide analysis by cation-exchange chromatography indicated that this processing step altered neither the methionine nor th ... | 1980 | 6255183 |
| herpes simplex type 1 infection of nonpermissive rat xc cells. | herpes simplex virus type 1 (hsv-1) infection of non permissive xc cells (a rat cell line transformed by rous sarcoma virus) was studied. using virus labeled with 3h-thymidine it was shown that adsorption is similar to that in a permissive system. by electron microscopy enveloped particles were observed in cytoplasmic vesicles in xc cells but not in the permissive system. however input viral dna was degraded both in non permissive cells (xc) and permissive cells (hep-2) and the degradation produ ... | 1980 | 6245633 |
| pyrophosphate analogues as inhibitors of herpes simplex virus type 1 dna polymerase. | twenty nine pyrophosphate analogues have been tested for their ability to inhibit herpes simplex virus type 1 (hsv-1) dna polymerase activity. the structural requirements for active inhibitors and their mechanism of action have been investigated. active compounds had two negatively charged groups at close proximity. the most active compounds contained free phosphono and/or carboxyl groups. apart from phosphonoformate and phosphonoacetate, which were strong inhibitors, some other analogues, 2-pho ... | 1980 | 6245699 |
| immunoglobulin g(fc)-binding receptors on virions of herpes simplex virus type 1 and transfer of these receptors to the cell surface by infection. | we have found that virions of herpes simplex virus type 1 have surface receptors capable of binding to the fc region of immunoglobulin g and have also shown that the previously identified fc-binding glycoprotein (designated ge) is present in extracts prepared from purified virions. the technique of affinity chromatography was used to detect ge. two approaches were used to demonstrate the presence of fc-binding receptors on intact virions. first, it was shown that [125i]-immunoglobulin g, but not ... | 1980 | 6246278 |
| participation of three major glycoprotein antigens of herpes simplex virus type 1 early in the infectious cycle as determined by antibody-dependent cell-mediated cytotoxicity. | tissue culture cells infected with herpes simplex virus type 1 synthesize three major glycoprotein antigens (ag-11, ag-8, and ag-6), which have been characterized by crossed immunoelectrophoresis. the three viral antigens have been identified as a mixture of ga and gb (ag-11), gd (ag-8), and gc (ag-6). recent findings have shown that antibodies directed to each of the three antigens individually are able to mediate antibody-dependent cell-mediated cytotoxicity when tissue culture cells late in t ... | 1980 | 6247286 |
| alterations in glycoprotein gb specified by mutants and their partial revertants in herpes simplex virus type 1 and relationship to other mutant phenotypes. | the tsb5 mutant of herpes simplex virus type 1 (hsv-1) strain hfem was shown previously to be temperature sensitive for accumulation of the mature form of glycoprotein gb, for production or activity of a factor required in virus-induced cell fusion, and for production of virions with normal levels of infectivity. in addition, a previous study showed that virions produced by tsb5 at permissive temperature were more thermolabile than hfem virions and contained altered gb that did not assume the di ... | 1980 | 6251260 |
| antiviral activity of antilipidemic compounds on herpes simplex virus type 1. | two antilipidemic compounds, clofibrate and procetofene, inhibited the replication of herpes simplex virus type 1 (hsv-1) in african green monkey kidney cells. clofibrate, at a concentration of 400 mumol/liter caused a 63% reduction (p < 0.001) in hsv-1 yield and at 100 mumol/liter caused a 62% reduction (p < 0.001) in plaque formation. two stereoisomeric analogs of clofibric acid, (-)- and (+)-desmethyl clofibric acid, also caused a significant inhibition of hsv-1 replication. procetofene at 5 ... | 1980 | 6255860 |
| erythro-9-(2-hydroxy-3-nonyl) adenine alone and in combination with 9-beta-d-arabinofuranosyladenine in treatment of systemic herpesvirus infections in mice. | although the antiviral activity of erythro-9-(2-hydroxy-3-nonyl)adenine, a potent adenosine deaminase inhibitor, against herpes simplex virus type 1 in cell culture was readily confirmed, the compound was found to be totally ineffective in the treatment of experimentally induced systemic herpes simplex virus type 1 infections in swiss mice. data were obtained, however, which clearly indicated that the antiviral potency of 9-beta-d-arabinofuranosyladenine in vivo could be enhanced by the co-admin ... | 1980 | 6255863 |
| selective inhibition of herpes simplex virus glycoprotein synthesis by a benz-amidinohydrazone derivative. | 1h-benz[f]indene-1.3(2h)dione-bis-amidinohydrazone (benzhydrazone) inhibited incorporation of 14c-glucosamine, 14c-fucose and 14c-mannose into glycoproteins of hep-2 cells infected with various strains of herpes simplex virus 1 (hsv-1) and impaired rna and protein synthesis to a low extent. these biochemical effects are very similar to those induced by glycosylation inhibitors such as tunicamycin, d-glucosamine and 2-deoxy-d-glucose. in contrast to these inhibitors, benzhydrazone reduced hsv gly ... | 1980 | 6255895 |
| immunogenicity of subviral herpes simplex virus type 1 preparation: protection of mice against intradermal challenge with type 1 and type 2 viruses. | an intradermal assay system for herpes simplex virus in the infected pinna (external ear) of mice was utilized for examining the immunogenicity of an experimental subviral herpes simplex type 1 virus vaccine. the administration of one dose of vaccine with adjuvant did not prevent the appearance of erythema; however, it markedly reduced the severity skin lesions induced by either type 1 or type 2 virus. in addition, neurological complications frequently seen in control mice were never observed in ... | 1980 | 6255896 |
| characterization of herpes simplex virus type 1 rna present in the absence of de novo protein synthesis. | we used herpes simplex virus type 1 (hsv-1) dna and restriction fragments of hsv-1 dna covalently coupled to cellulose as a reagent to isolate for further characterization the major and minor hsv-1 immediate-early mrna species in hela cells infected and maintained in the absence of de novo protein synthesis. five major and several minor immediate-early mrna species were characterized. one major species was a 4.2-kilobase mrna mapping in the tr(s)/ir(s) region with its 3' end distal to the u(s) r ... | 1980 | 6246265 |
| infections of susceptible and resistant mouse strains with herpes simplex virus type 1 and 2. | the spread of hsv of type 1 and 2 was investigated after intraperitoneal, intraplantar and intracerebral infections of resistant (c57/bl) and susceptible (nmri) mice. the virus spreads after i.p. infection to the spleen and the liver to the same extent in both strains of mice. however, virus is eliminated earlier in resistant mice. intracerebral infections revealed a peculiar type of resistance of c57/bl mice especially for type 2 of hsv. hsv multiplies in the thymus at the early stage of infect ... | 1980 | 6251783 |
| influence of 2,4,6-triamino-pyrimidyl-5-azobenzene on the activities of acid phosphatase, alkaline phosphatase and lactate dehydrogenase in cell cultures infected with herpes simplex virus type 1. | treatment of vero cells with 2,4,6-triamino-pyridimyl-5-azobenzene (10 gamma/ml) one hour after inoculation of herpes simplex virus type 1 limits the influence of the virus infection, consisting in the enchancement of the activities of alkaline phosphatase, acid phosphatase and ldh (especially of the latter two enzymes) and in changes in the isoenzyme patterns of acid and alkaline phosphatases and of peroxidase. in general the influence of the drug is expressed both by the fact that enzyme level ... | 1980 | 6250280 |