Publications

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cortico-cortical connectivity within ferret auditory cortex.despite numerous studies of auditory cortical processing in the ferret (mustela putorius), very little is known about the connections between the different regions of the auditory cortex that have been characterized cytoarchitectonically and physiologically. we examined the distribution of retrograde and anterograde labeling after injecting tracers into one or more regions of ferret auditory cortex. injections of different tracers at frequency-matched locations in the core areas, the primary aud ...201525845831
awake vs. anesthetized: layer-specific sensory processing in visual cortex and functional connectivity between cortical areas.during general anesthesia, global brain activity and behavioral state are profoundly altered. yet it remains mostly unknown how anesthetics alter sensory processing across cortical layers and modulate functional cortico-cortical connectivity. to address this gap in knowledge of the micro- and mesoscale effects of anesthetics on sensory processing in the cortical microcircuit, we recorded multiunit activity and local field potential in awake and anesthetized ferrets (mustela putoris furo) during ...201525833839
constant rate infusion of glucagon as an emergency treatment for hypoglycemia in a domestic ferret (mustela putorius furo).a 3-year-old female domestic ferret (mustela putorius furo) with an insulinoma was treated because of a hypoglycemic crisis prior to scheduled pancreatectomy with concurrent nodulectomy.201525632820
a retrospective study of 17 cases of mycobacteriosis in domestic ferrets (mustela putorius furo) between 2005 and 2013.from 2005 to 2013, 17 ferrets were diagnosed with mycobacteriosis at the authors' practices. clinical findings included ocular, respiratory, and digestive abnormalities. diagnosis was based on histopathology, specific histochemical stains, and/or on polymerase chain reaction. all bacteria identified belonged to the nontuberculous mycobacterium complex. several treatment protocols were attempted, frequently based on the use of enrofloxacin. in all, 3 ferrets were considered cured. mycobacteriosis ...201532288684
morphological differentiation of the skull in two closely-related mustelid species (carnivora: mustelidae).alexei v. abramov, andrey yu. puzachenko, and igor l. tumanov (2016) a morphological differentiation (i.e., the amount of morphological space occupied) in two polecat species, mustela putorius and m. eversmanii, has been studied. these closely related species are similar in the body size, the age of origin, and many aspects of their natural history. we have used cranial characters to estimate some parameters of morphological diversity, to compare 'morphological niche breadth' occupied by polecat ...201631966146
[the characteristics of water metabolism and the resistance to dehydration of representatives of the family of mustelids (carnivora: mustelidae)].food consumption, total water intake, urine volume and concentration, and water content in feces were determined under the conditions of free access to drinking water and in its absence in some mustelids that inhabit territories with different water accessibility: mustela putorius, m. eversmanni, m. lutreola, and m. vison. specific features of water metabolism and resistance to dehydration in the studied species corresponded fairly well to differences in their ecology.20167735034
serological survey of toxoplasma gondii and besnoitia besnoiti in a wildlife conservation area in southern portugal.toxoplasma gondii and besnoitia besnoiti are closely related apicomplexan protozoa. t. gondii is a zoonotic pathogen which may cause serious disease in man and warm-blooded animals, including wild species. b. besnoiti causes bovine besnoitiosis, an emergent disease in europe, which is linked to important production losses. unlike t. gondii, the life cycle of b. besnoiti remains a mystery, since the definitive host has not yet been identified. the aim of this work was to determine the seroprevale ...201631014504
structural and functional connectivity between the lateral posterior-pulvinar complex and primary visual cortex in the ferret.the role of higher-order thalamic structures in sensory processing remains poorly understood. here, we used the ferret (mustela putorius furo) as a novel model species for the study of the lateral posterior (lp)-pulvinar complex and its structural and functional connectivity with area 17 [primary visual cortex (v1)]. we found reciprocal anatomical connections between the lateral part of the lp nucleus of the lp-pulvinar complex (lpl) and v1. in order to investigate the role of this feedback loop ...201626505737
neoplastic diseases in the domestic ferret (mustela putorius furo) in italy: classification and tissue distribution of 856 cases (2000-2010).the aim of this study was to describe the prevalence and tissue distribution of neoplasms in italian ferrets, compared to the epidemiological data previously reported in usa and japan.201627919252
s-phase duration is the main target of cell cycle regulation in neural progenitors of developing ferret neocortex.the evolutionary expansion of the neocortex primarily reflects increases in abundance and proliferative capacity of cortical progenitors and in the length of the neurogenic period during development. cell cycle parameters of neocortical progenitors are an important determinant of cortical development. the ferret (mustela putorius furo), a gyrencephalic mammal, has gained increasing importance as a model for studying corticogenesis. here, we have studied the abundance, proliferation, and cell cyc ...201625963823
pyogranulomatous panophthalmitis with systemic coronavirus disease in a domestic ferret (mustela putorius furo).a 15-month-old spayed female ferret (mustela putorius furo) presented for lethargy and weight loss of 2 weeks duration. upon physical examination, a 2-mm-diameter focal area of opacity was noted in the left cornea. in addition, the ferret was quiet, in poor body condition, and dehydrated. a complete blood count and plasma biochemistry revealed a severe nonregenerative anemia, azotemia, hyperproteinemia, hypoalbuminemia, and mild hyperphosphatemia and hyperchloremia. urinalysis revealed hyposthen ...201625918975
imaging findings in 14 domestic ferrets (mustela putorius furo) with lymphoma.lymphoma is the most common malignant neoplasia in domestic ferrets, mustela putorius furo. however, imaging findings in ferrets with lymphoma have primarily been described in single case reports. the purpose of this retrospective study was to describe imaging findings in a group of ferrets with confirmed lymphoma. medical records were searched between 2002 and 2012. a total of 14 ferrets were included. radiographs (n = 12), ultrasound (n = 14), computed tomography (ct; n = 1), and magnetic reso ...201623738830
[population aspects of sexual dimorphism in guild of the mustelidae: mustela lutreola, neovison vison, mustela putorius, martes martes as an example].size sexual dimorphism was investigated on 695 skulls of four mustelidae species. by extent of increasing of differences between sexes the species are placed in following order: european pine marten (martes martes), european mink (mustela lutreola), american mink (neovison vison), and european polecat (mustela putorius). extent of the dimorphism characterizes ecological plasticity of the species and is population characteristic. it is shown that m. martes takes specific and relatively narrow eco ...201623662464
[water and energy metabolism in representatives of the genus martes and mustela (mammalia: mustelidae)].the quantities of consumed food and water, quantity and moisture content of faeces, as well as quantity and concentration of excreted urine were determined in representatives of martes--marten (m. martes) and sable (m. zibellina)--as well as in polecat (mustela putorius). under the same cage conditions and free access to food, all three species had similar energy value of the daily diet. however, the level of drinking water consumption and the ratio between the quantities of arriving water and e ...201612942756
diagnosis and management of polycythemia vera in a ferret (mustela putorius furo).a 5-y-old female ferret (mustela putorius furo) was evaluated for diarrhea, anorexia, and lethargy for 1 wk. only mild dehydration was detected on physical examination. cbc analysis revealed marked erythrocytosis with an unremarkable plasma biochemistry panel; follow-up cbc analyses revealed a consistent primary erythrocytosis. whole-body radiographs and abdominal ultrasonography were unremarkable except for a small nephrolith in the right kidney and a small cyst in the left kidney. the plasma e ...201628304249
oral and dental diseases in a population of domestic ferrets (mustela putorius furo).domestic ferrets have been used for many purposes, but recently their popularity as companion animals has increased greatly. however, data on their oral and dental diseases are rare. the objective of this study was therefore to describe oral and dental diseases in a population of client-owned domestic ferrets.201627604062
clinical, cytological, histological and immunohistochemical features of cutaneous mast cell tumours in ferrets (mustela putorius furo).cutaneous mast cell tumours (cmcts) are one of the most common cutaneous tumours in ferrets (mustela putorius furo). however, limited information is available regarding cytological and histological features of these tumours and studies evaluating kit expression are lacking in this species. the aims of this prospective study were to describe the most common clinical, cytological and histological features of cmcts in ferrets and to compare the usefulness of different staining techniques in the dia ...201627578524
comparison of digital rectal and microchip transponder thermometry in ferrets (mustela putorius furo).body temperature is a common physiologic parameter measured in both clinical and research settings, with rectal thermometry being implied as the 'gold standard.' however, rectal thermometry usually requires physical or chemical restraint, potentially causing falsely elevated readings due to animal stress. a less stressful method may eliminate this confounding variable. the current study compared 2 types of digital rectal thermometers-a calibrated digital thermometer and a common digital thermome ...201627177569
polyostotic lymphoma in a ferret (mustela putorius furo).an 8.5-year-old, neutered female ferret (mustela putorius furo) was presented with tachypnoea, polyuria, polydipsia, anorexia and depression. radiographs revealed multiple osteolytic lesions of the bone, characterized cytologically by the infiltration of medium- to large-sized lymphocytes. the animal was humanely destroyed and post-mortem examination revealed multifocal masses obliterating the bone marrow of the mandible, right and left humeri and femur, and consisting of an infiltrative populat ...201627147110
collateral circulation in ferrets (mustela putorius) during temporary occlusion of the caudal vena cava.objective to determine whether extent of collateral circulation would change during temporary occlusion of the caudal vena cava (cvc) in ferrets (mustela putorius), a pressure change would occur caudal to the occlusion, and differences would exist between the sexes with respect to those changes. animals 8 adult ferrets (4 castrated males and 4 spayed females). procedures ferrets were anesthetized. a balloon occlusion catheter was introduced through a jugular vein, passed into the cvc by use of f ...201627111022
histology and immunohistochemistry of severe inflammatory bowel disease versus lymphoma in the ferret (mustela putorius furo).inflammatory bowel disease (ibd) is a common disorder of ferrets (mustela putorius furo) that may progress to lymphoma. although routine histology is used to distinguish between these diseases, misclassifications may occur. immunohistochemistry (ihc) is commonly used to distinguish between ibd and lymphoma in small animals. the objective of our study was to determine the agreement in the diagnosis reached solely using hematoxylin and eosin (he)-stained, full-thickness sections versus using a com ...201627026106
histological and immunohistochemical features of histiocytic sarcoma in four domestic ferrets (mustela putorius furo).four cases of histiocytic sarcoma in domestic ferrets (mustela putorius furo) are described in the present study. tumor samples obtained from the abdominal viscera, including the spleen, were submitted for histologic examination. microscopically, poorly demarcated masses contained numerous round- to pleomorphic-shaped cells with coarsely vacuolated and eosinophilic cytoplasm. bizarre, binucleated tumor cells and multinucleated giant tumor cells with low phagocytic activity were commonly observed ...201626965237
oral malignant melanoma in a ferret ( mustela putorius furo).oral malignant melanomas are one of the most common oral malignant neoplasms in dogs but are rare in other domesticated species. this case report describes the clinical manifestations and histological appearance of oral melanoma in a ferret ( mustela putorius furo). to the authors' knowledge, this is the first published description of a clinical case and histopathological findings of oral melanoma in this species.201628326981
a post-mortem study of respiratory disease in small mustelids in south-west england.stoat (mustela erminea) and weasel (mustela nivalis) populations in south-west england are declining whilst polecats (mustela putorius), absent for over a century, are increasing. little is known about the health status of these species nationally. this study aimed at investigating respiratory disease in specimens found dead in south-west england.201627052849
possible role of fish and frogs as paratenic hosts of dracunculus medinensis, chad.copepods infected with dracunculus medinensis larvae collected from infected dogs in chad were fed to 2 species of fish and tadpoles. although they readily ingested copepods, neither species of fish, nile tilapia (oreochromis niloticus) nor fathead minnow (pimephalis promelas), were found to harbor dracunculus larvae when examined 2-3 weeks later. tadpoles ingested copepods much more slowly; however, upon examination at the same time interval, tadpoles of green frogs (lithobates [rana] clamitans ...201627434418
the domestic ferret (mustela putorius furo) as a lethal infection model for 3 species of ebolavirus.small-animal models have been developed for several filoviridae species; however, serial adaptation was required to produce lethal infection. these adapted viruses have sequence changes in several genes, including those that modulate the host immune response. nonhuman primate models do not require adaptation of filoviruses. here, we describe lethal models of disease for bundibugyo, sudan, and zaire species of ebolavirus in the domestic ferret, using wild-type nonadapted viruses. pathologic featu ...201627354371
ferrets as a novel animal model for studying human respiratory syncytial virus infections in immunocompetent and immunocompromised hosts.human respiratory syncytial virus (hrsv) is an important cause of severe respiratory tract disease in immunocompromised patients. animal models are indispensable for evaluating novel intervention strategies in this complex patient population. to complement existing models in rodents and non-human primates, we have evaluated the potential benefits of an hrsv infection model in ferrets (mustela putorius furo). nine- to 12-month-old hrsv-seronegative immunocompetent or immunocompromised ferrets wer ...201627314379
evidence of disseminated infection by mycobacterium avium subspecies hominissuis in a pet ferret (mustela putorius furo).the infection caused by the zoonotic opportunistic pathogen mycobacterium avium subsp. hominissuis (mah) was reported for the first time in a pet ferret. both owners were hiv-positive. euthanasia of the pet was recommended due to medical reasons and as a preventive action. disseminated and open tuberculosis lesions were observed in the gastrointestinal and respiratory systems of the ferret. ecographic and radiographic surveys showed a severe generalized lymphadenopathy, strong thickening of the ...201627892873
detection, prevalence and phylogenetic relationships of demodex spp and further skin prostigmata mites (acari, arachnida) in wild and domestic mammals.this study was conceived to detect skin mites in social mammals through real-time qpcr, and to estimate taxonomic demodex and further prostigmata mite relationships in different host species by comparing sequences from two genes: mitochondrial 16s rrna and nuclear 18s rrna. we determined the mite prevalence in the hair follicles of marmots (13%) and bats (17%). the high prevalence found in marmots and bats by sampling only one site on the body may indicate that mites are common inhabitants of th ...201627802314
an atypical distribution of lactate dehydrogenase isoenzymes in the hooded seal (cystophora cristata) brain may reflect a biochemical adaptation to diving.the brains of some diving mammals can withstand periods of severe hypoxia without signs of deleterious effects. this may in part be due to an enhanced cerebral capacity for anaerobic energy production. here, we have tested this hypothesis by comparing various parameters of the lactate dehydrogenase (ldh) in the brain of the hooded seal (cystophora cristata) with those in the brains of the ferret (mustela putorius furo) and mouse (mus musculus). we found that mrna and protein expression of lactat ...201626820264
brains of native and alien mesocarnivores in biomonitoring of toxic metals in europe.mercury (hg), lead (pb) and cadmium (cd) are involved in mammalian brain damage. however, little is known about pb and cd brain levels in wildlife that reflect the geochemical background. the aims of the study include the estimation of hg, pb and cd concentrations, and the determination of relationships between these elements in the brains of 94 mesocarnivores. road-killed or hunted animals were obtained from north-western poland near the polish-german border. the investigation covered the nativ ...201627513467
when the brain goes diving: transcriptome analysis reveals a reduced aerobic energy metabolism and increased stress proteins in the seal brain.during long dives, the brain of whales and seals experiences a reduced supply of oxygen (hypoxia). the brain neurons of the hooded seal (cystophora cristata) are more tolerant towards low-oxygen conditions than those of mice, and also better survive other hypoxia-related stress conditions like a reduction in glucose supply and high concentrations of lactate. little is known about the molecular mechanisms that support the hypoxia tolerance of the diving brain.201627507242
pulse of inflammatory proteins in the pregnant uterus of european polecats (mustela putorius) leading to the time of implantation.uterine secretory proteins protect the uterus and conceptuses against infection, facilitate implantation, control cellular damage resulting from implantation, and supply pre-implantation embryos with nutrients. unlike in humans, the early conceptus of the european polecat (mustela putorius; ferret) grows and develops free in the uterus until implanting at about 12 days after mating. we found that the proteins appearing in polecat uteri changed dramatically with time leading to implantation. seve ...201728405395
diagnosis and management of pyothorax in a domestic ferret (mustela putorius furo).to describe the diagnosis, management, and outcome of pyothorax in a domestic ferret (mustela putorius furo).201728485830
nasal infection of enterovirus d68 leading to lower respiratory tract pathogenesis in ferrets (mustela putorius furo).data from ev-d68-infected patients demonstrate that pathological changes in the lower respiratory tract are principally characterized by severe respiratory illness in children and acute flaccid myelitis. however, lack of a suitable animal model for ev-d68 infection has limited the study on the pathogenesis of this critical pathogen, and the development of a vaccine. ferrets have been widely used to evaluate respiratory virus infections. in the current study, we used ev-d68-infected ferrets as a ...201728489053
transmission of mycobacterium xenopi to a pet albino ferret (mustela putorius furo) from a domestic aquarium.a three-year-old ferret presented with a three-month history of rapid clinical deterioration necessitating euthanasia shortly after initial veterinary assessment. postmortem pcr testing confirmed mycobacterium xenopi which is most commonly identified in amphibians, reptiles and aquatic life. infection of a captive-bred domestic ferret is highly unusual. a collaborative effort involving medical doctors, clinical veterinarians and veterinary pathologists investigated the potential sources of human ...201728774941
genetic and physicochemical analyses of a novel ferret hepatitis e virus, and clinical signs of infection after birth.a novel cluster of five ferret hepatitis e virus (hev) strains was detected from nine laboratory ferrets (mustela putorius furo) imported from a ferret farm in the u.s. our detection of ferret hev rna and anti-hev antibodies, and alanine aminotransferase (alt) value assessment indicated that all of the 9 ferrets were infected with ferret hev, and that the infection exhibited three patterns: sub-clinical infection (n=2), acute hepatitis (n=6) and persistent infection (n=1). next-generation sequen ...201728347753
occurrence and molecular genotyping of giardia duodenalis and cryptosporidium spp. in wild mesocarnivores in spain.there is a surprisingly scarce amount of epidemiological and molecular data on the prevalence, frequency, and diversity of the intestinal protozoan parasites giardia duodenalis and cryptosporidium spp. in wildlife in general and mesocarnivore species in particular. consequently, the extent of the cyst/oocyst environmental contamination attributable to these wild host species and their potential implications for public veterinary health remain largely unknown. in this molecular epidemiological su ...201728215875
imaging diagnosis-radiography and ultrasonography of bilateral congenital ureterovesical junction stenosis causing hydronephrosis and hydroureter in a ferret (mustela putorius furo).an 8-month-old neutered male ferret was presented for vaccination and preventive treatment for adrenal gland disease. abdominal ultrasound revealed severe bilateral hydronephrosis and hydroureter. excretory urography and negative contrast cystography showed bilateral ureterovesical junction stenosis. positive retrograde cystography demonstrated right ureteral reflux. a subcutaneous ureteral bypass was placed in the left kidney; however, it had to be removed 3 months later due to an obstruction a ...201727490371
lachrymal gland basal cell adenocarcinoma in a ferret (mustela putorius furo).a 1 cm diameter mass was detected in the caudal superotemporal area of the left eye of a 6-year-old neutered male ferret (mustela putorius furo). the mass and the left eye were removed surgically. microscopical examination revealed a tumour of the adnexal gland of the eye that had invaded the surrounding ocular muscle. the tumour was composed of basal-type epithelial cells arranged in a solid, or occasionally tubular, pattern. immunohistochemically, the tumour cells expressed cytokeratin and p63 ...201727388784
generation of monoclonal antibodies against immunoglobulin proteins of the domestic ferret (mustela putorius furo).the domestic ferret (mustela putorius furo) serves as an animal model for the study of several viruses that cause human disease, most notably influenza. despite the importance of this animal model, characterization of the immune response by flow cytometry (fcm) is severely hampered due to the limited number of commercially available reagents. to begin to address this unmet need and to facilitate more in-depth study of ferret b cells including the identification of antibody-secreting cells, eight ...201728286781
cold exposure down-regulates immune response pathways in ferret aortic perivascular adipose tissue.perivascular adipose tissue (pvat) surrounds blood vessels and releases paracrine factors, such as cytokines, which regulate local inflammation. the inflammatory state of pvat has an important role in vascular disease; a pro-inflammatory state has been related with atherosclerosis development, whereas an anti-inflammatory one is protective. cold exposure beneficially affects immune responses and, could thus impact the pathogenesis of cardiovascular diseases. in this study, we investigated the ef ...201728251235
invasive histiocytic sarcoma of the lumbar spine in a ferret (mustela putorius furo).this report describes the history, clinical examination and histopathology of a histiocytic sarcoma in a domestic ferret. clinical signs were acute paraplegia and dysuria. physical examination revealed a firm, smooth, touch-sensitive mass in and around the lumbar vertebral column. neurologic examination was consistent with a lesion between spinal cord segments t3 and l3. magnetic resonance images revealed bone lesions of l2 and l3 combined with compression of the spinal cord due to a homogenous, ...201728160308
hodgkin's-like lymphoma in a ferret (mustela putorius furo).a 7-year-old castrated male ferret developed unilateral cervical lymphadenomegaly over a 1-month period. histological examination revealed proliferation of tumor cells in a diffuse and partially nodular pattern. the tumor cells were predominantly hodgkin cells and binucleated reed-sternberg cells, characterized by abundant, clear, vacuolated cytoplasm, pleomorphic, ovoid nuclei with thick nuclear membranes and distinct nucleoli. multinucleated cells, resembling lymphocytic and histiocytic (l&h) ...201728804091
diagnosis and treatment of esophageal foreign body or stricture in three ferrets (mustela putorius furo).case description 3 ferrets (mustela putorius furo), aged 1 to 2 years, were referred for evaluation of a 4-day to 2-week history of gastrointestinal signs, including anorexia, regurgitation, and vomiting. clinical findings all 3 ferrets had clinical signs suggestive of dysphagia or esophagitis on initial examination. esophagoscopy, barium-contrast esophagography, or both revealed foreign bodies with mucosal inflammation in 1 patient and an esophageal foreign body with stricture in 2 patients. on ...201728763273
molecular investigations of development and diseases of the brain of higher mammals using the ferret.the brains of higher mammals such as primates and carnivores contain well-developed unique brain structures. uncovering the physiological functions, developmental mechanisms and evolution of these brain structures would greatly facilitate our understanding of the human brain and its diseases. although the anatomical and electrophysiological features of these brain structures have been intensively investigated, our knowledge about their molecular bases is still limited. to overcome this limitatio ...201728496051
delineating morbillivirus entry, dissemination and airborne transmission by studying in vivo competition of multicolor canine distemper viruses in ferrets.identification of cellular receptors and characterization of viral tropism in animal models have vastly improved our understanding of morbillivirus pathogenesis. however, specific aspects of viral entry, dissemination and transmission remain difficult to recapitulate in animal models. here, we used three virologically identical but phenotypically distinct recombinant (r) canine distemper viruses (cdv) expressing different fluorescent reporter proteins for in vivo competition and airborne transmi ...201728481926
pneumatosis coli in a domestic ferret (mustela putorius furo).a 4-year-old spayed female ferret was presented with acute diarrhea and partial anorexia. pneumatosis coli and segmental enteropathy were identified by ultrasonography and radiography. fecal culture did not identify any pathogenic bacteria. medical management of concurrent diseases and antibiotic therapy resulted in resolution of clinical signs and pneumatosis coli.201728373731
regional distribution of cholinergic, catecholaminergic, serotonergic and orexinergic neurons in the brain of two carnivore species: the feliform banded mongoose (mungos mungo) and the caniform domestic ferret (mustela putorius furo).the nuclear organization of the cholinergic, catecholaminergic, serotonergic and orexinergic neurons in the brains of two species of carnivore, the banded mongoose (mungos mungo) and domestic ferret (mustela putorius furo), is presented. the banded mongoose belongs to the feliform suborder and the domestic ferret to the caniform suborder, having last shared a common ancestor approximately 53 million years ago; however, they have a very similar overall morphology and life history, presenting an i ...201728416460
exposure of stone marten (martes foina) and polecat (mustela putorius) to anticoagulant rodenticides: effects of regulatory restrictions of rodenticide use.when anticoagulant rodenticides (ars) are used to control rodent populations there is also a widespread secondary exposure of non-target predators to ars. to reduce secondary exposure, regulatory restrictions in ar usage were tightened in denmark in 2011. the restrictions included the cessation of ar use for plant protection and any use away from buildings, as well as limitations in private consumers' access to ars. to quantify and evaluate the efficiency of the regulatory measures to reduce sec ...201728898942
ferret oncology: diseases, diagnostics, and therapeutics.neoplastic disease is common in ferrets. approximately half of all tumors diagnosed in ferrets are located in the endocrine or hemolymphatic system. many factors may influence the choice of treatment. medical management of adrenal tumors has a greater disease-free period compared to adrenalectomy. in ferrets with an insulinoma, no difference is seen in the mean survival time of medically and surgically treated patients. aside from medical and surgical treatment modalities, chemotherapy and radia ...201727890288
infection of ferrets with influenza virus elicits a light chain-biased antibody response against hemagglutinin.the domestic ferret (mustela putorius furo) is a commonly used animal model for the study of influenza virus infection and vaccination. recently, our group has developed murine mabs with specificity for the κ (igκ) and λ (igλ) l chains of ferret ig. these mabs were used to quantify the abundance of igκ and igλ in serum and to evaluate l chain usage of the ab response against the hemagglutinin (ha) protein elicited by influenza infection. after influenza a infection of immunologically naive ferre ...201729079697
the influence of diet on masticatory motor patterns in musteloid carnivorans: an analysis of jaw adductor activity in ferrets (mustela putorius furo) and kinkajous (potos flavus).broad similarities in the timing of jaw adductor activity driving jaw movements across distantly related and morphologically disparate species have led to the hypothesis that mammalian masticatory motor patterns are conserved. however, some quantitative analyses also suggest that masticatory motor patterns have evolved in concert with dietary and/or morphological specialization. here, we assess this relationship in two closely related carnivoran species with divergent diets and morphology: carni ...201729441707
non-invasive blood pressure measurement in ferrets (mustela putorius furo) using high definition oscillometry.this study was conducted to validate the use of high definition oscillometry (hdo) for non-invasive blood pressure (nibp) measurements in ferrets and to establish reference ranges for nibp in minimally sedated, healthy, young adult ferrets (<4years of age). the bias, limits of agreement and correlation for hdo compared to direct arterial blood pressure (dabp) measurement were established in 14 anaesthetised ferrets. measurements were performed at the forelimb, hind limb and tail under hypo- (<11 ...201729153109
extreme tetralogy of fallot with polycythemia in a ferret (mustela putorius furo).a 5-month-old, intact male ferret (mustela putorius furo) was presented with apathy, tachypnea and exercise intolerance. on initial physical examination, tachypnea and cyanosis were the two primary clinical signs detected. the complete blood count also revealed a severe polycythemia, with lymphopenia, neutrophilia and eosinophilia. further diagnostic imaging tests were performed. the thoracic radiographs revealed cardiomegaly and a diffuse alveolar pulmonary pattern. consequently, an echocardiog ...201728992910
long-term increase in secondary exposure to anticoagulant rodenticides in european polecats mustela putorius in great britain.as a result of legal protection and population recovery, european polecats (mustela putorius) in great britain are expanding into areas associated with greater usage of second-generation anticoagulant rodenticides (sgars). we analysed polecat livers collected from road casualties from 2013 to 2016 for residues of five sgars. we related variation in residues to polecat traits and potential exposure pathways, by analysing stable isotopes of carbon (δ13c) and nitrogen (δ15n) in their whiskers. 54 o ...201829438955
molecular identification of sarcocystis lutrae (apicomplexa: sarcocystidae) in muscles of five species of the family mustelidae.carnivores usually act as definitive hosts of sarcocystis species. however, the number of reports on sarcocyst formation in musculature of predators is on the increase. in the present study, muscle samples of 68 mustelids collected in lithuania were examined for sarcocysts of sarcocystis species. sarcocysts were detected in diaphragm, tongue and limb muscles of ten animals (14.7%) but were not discovered in the heart. based on 18s rdna, 28s rdna, cox1 and its1 sequence analysis, sarcocystis lutr ...201829700640
mycobacterium genavense infection in a domestic ferret (mustela putorius furo).mycobacterium genavense infection was diagnosed in an adult ferret with ptosis of the left eye, a proliferative lesion of the conjunctiva of the nictitating membrane, conjunctival swelling, and tumefaction of the periorbital tissues with a watery ocular discharge and the presence of a retrobulbar mass. the diagnosis was based on characteristic cytology of the retrobulbar mass and left mandibular lymph node that revealed granulomatous inflammation. ziehl-neelsen staining showed the presence of po ...201830502861
trypsinogen isoforms in the ferret pancreas.the domestic ferret (mustela putorius furo) recently emerged as a novel model for human pancreatic diseases. to investigate whether the ferret would be appropriate to study hereditary pancreatitis associated with increased trypsinogen autoactivation, we purified and cloned the trypsinogen isoforms from the ferret pancreas and studied their functional properties. we found two highly expressed isoforms, anionic and cationic trypsinogen. when compared to human cationic trypsinogen (prss1), ferret a ...201830305676
molecular investigations of the development and diseases of cerebral cortex folding using gyrencephalic mammal ferrets.folds of the cerebral cortex (gyri and sulci) are among the most important properties of the mammalian brain. uncovering the physiological roles, developmental mechanisms and evolution of the cortical folds would greatly facilitate our understanding of the human brain and its diseases. although the anatomical features of the cortical folds have been intensively investigated, our knowledge about their molecular bases is still limited. to overcome this limitation, we recently established rapid and ...201830175769
dermatophytic pseudomycetomas in two ferrets (mustela putorius furo).dermatophytic pseudomycetoma is an atypical form of dermatophytosis where the infection is located in the deep dermal and subcutaneous tissues. although rare, it is most commonly associated with microsporum canis or trichophyton sp. it has been reported in cats, dogs and horses.201830091502
marburg and ravn viruses fail to cause disease in the domestic ferret (mustela putorius furo).the domestic ferret was recently described as a uniformly lethal model for 3 species of ebolavirus. more importantly, this new model utilizes nonadapted wild-type ebolaviruses. here, in a proof-of-concept study, we infected ferrets with different variants of the closely related marburg and ravn viruses using different doses and routes of exposure. although ferrets produced a neutralizing humoral response to challenge, we did not observe disease or viremia in any animal. the lack of disease in fe ...201829955887
founder events, isolation, and inbreeding: intercontinental genetic structure of the domestic ferret.domestication and breeding for human-desired morphological traits can reduce population genetic diversity via founder events and artificial selection, resulting in inbreeding depression and genetic disorders. the ferret (mustela putorius furo) was domesticated from european polecats (m. putorius), transported to multiple continents, and has been artificially selected for several traits. the ferret is now a common pet, a laboratory model organism, and feral ferrets can impact native biodiversity. ...201829875811
pancreatitis and systemic coronavirus infection in a ferret (mustela putorius furo).a 1-y-old spayed female ferret (mustela putorius furo) was referred for additional diagnostic evaluation after physical examination by the referring veterinarian revealed a cranial abdominal mass. the ferret had a 2-wk history of inappetence, weight loss, and lethargy. on presentation, the ferret was thin, and an approximately 3-cm mass was palpable in the cranial abdomen. no other abnormalities were noted. abdominal ultrasonography confirmed the presence of a soft-tissue structure, with a moder ...201829776456
variability of serum aldosterone concentrations in pet ferrets (mustela putorius furo).objective to explore sources of serum aldosterone concentration variability in a population of healthy and diseased ferrets, determine a preliminary 1 -sided reference interval for serum aldosterone concentration in healthy ferrets, and identify a decision limit to differentiate healthy from diseased ferrets on the basis of serum aldosterone concentration. design prospective threshold definition and diagnostic accuracy study. animals 78 healthy (n = 56) and diseased (22) ferrets. procedures seru ...201829772966
validation of a radioimmunoassay of serum trypsin-like immunoreactivity in ferrets.measurement of serum trypsin-like immunoreactivity (tli) is used to assess exocrine pancreatic function in dogs and cats. ferrets ( mustela putorius furo) serve as valuable animal models for human diseases such as cystic fibrosis and other pulmonary diseases, and may be a useful model of other diseases including pancreatitis. we developed and analytically validated a competitive radioimmunoassay (ria) for measurement of tli in ferret serum by determination of analytical sensitivity, assay linear ...201829717637
chylous ascites associated with abdominal trauma and intestinal resection-anastomosis in a pet ferret (mustela putorius furo).case description a 10-week-old 0.73-kg (1.6-lb) castrated male domestic ferret (mustela putorius furo) was referred for exploratory laparotomy because of pneumoperitoneum and possible septic peritonitis after being bitten by the owner's dog. clinical findings abdominal exploration revealed a large laceration of the duodenum, tears of the jejunal mesentery, and 2 small tears in the abdominal wall. chylous abdominal effusion developed 48 hours after surgery. treatment and outcome postoperative car ...201829701519
electrochemotherapy with bleomycin of different types of cutaneous tumours in a ferret (mustela putorius furo).mast cell tumour, sebaceous gland adenoma, and less common squamous papilloma are skin tumours in ferrets (mustela putorius furo), and early excisional surgery is usually the treatment of choice. the aim of our study was to investigate the effectiveness of electrochemotherapy (ect), a new, minimally invasive non-surgical method, as first treatment option of different types of ferret skin tumours located on surgically difficult sites.201829520211
filarial dermatitis caused by filaria taxideae in domestic ferrets (mustela putorius furo) from the western united states.filaria taxideae is a common subcutaneous filarial parasite of american badgers (taxidea taxus) that has also been reported in skunks in the western usa. dermatitis associated with this parasite has been reported in badgers and skunks; however, many individuals have no gross lesions. with the exception of a captive red panda in california, there have been no reports of f. taxidae infection in domestic or exotic/pet animals. in this case series, we document f. taxideae in domestic ferrets from ca ...201831014722
prevalence of capillaria plica in danish wild carnivores.capillaria plica is a parasitic nematode belonging to the family capillariidae. the adult parasites reside in the urinary tract of wild and domestic canines. the infection is most often asymptomatic, but can cause a wide range of symptoms including urinary bladder inflammation, pollacisuria, dysuria and hematuria. canines acquire the infection by ingesting the intermediate host, the earthworm (lumbricidae). epidemiological studies on c. plica infection in wildlife are few and only one previous d ...201830302310
causes of mortality and morbidity in free-ranging mustelids in switzerland: necropsy data from over 50 years of general health surveillance.although mustelids occur worldwide and include a wide range of species, little is known about the diseases affecting them. mustelids have regularly been submitted for post mortem investigation in the framework of the program for general wildlife health surveillance in switzerland, which has been in place for nearly 60 years. we performed a retrospective analysis of the necropsy reports on mustelids submitted to the diagnostic service of the university of bern. the aims of this study were to pres ...201829921290
assessment of influenza virus hemagglutinin stalk-specific antibody responses.animal models are essential to examine the pathogenesis and transmission of influenza viruses and for preclinical evaluation of influenza virus vaccines. among the animal models used in influenza virus research, the domestic ferret (mustela putorius furo) is the gold standard. as seen in humans, infection with influenza virus or immunization with an influenza virus vaccine induces humoral and cellular immunity in ferrets that provides protection against infection by an antigenically similar infl ...201830151588
aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.the human cerebral cortex is distinguished by its large size and abundant gyrification, or folding. however, the evolutionary mechanisms that drive cortical size and structure are unknown. although genes that are essential for cortical developmental expansion have been identified from the genetics of human primary microcephaly (a disorder associated with reduced brain size and intellectual disability) 1 , studies of these genes in mice, which have a smooth cortex that is one thousand times small ...201829643508
role of mustelids in the life-cycle of ixodid ticks and transmission cycles of four tick-borne pathogens.elucidating which wildlife species significantly contribute to the maintenance of ixodes ricinus populations and the enzootic cycles of the pathogens they transmit is imperative in understanding the driving forces behind the emergence of tick-borne diseases. here, we aimed to quantify the relative contribution of four mustelid species in the life-cycles of i. ricinus and borrelia burgdorferi (sensu lato) in forested areas and to investigate their role in the transmission of other tick-borne path ...201830458847
paraplegia in a domestic ferret (mustela putorius furo) secondary to metastatic adrenocortical carcinoma with myxoid differentiation.neurologic disease is a common presentation of domestic ferrets (mustela putorius furo) with infectious, neoplastic, and traumatic etiologies documented. adrenocortical neoplasia is also well documented in domestic ferrets, with metastatic lesions rarely described. this case report describes an abnormal presentation of adrenocortical adenocarcinoma with myxoid differentiation and subsequent metastasis to the spinal cord and vertebral bodies, resulting in hind limb paraplegia in an adult spayed f ...201932288686
theta oscillations organize spiking activity in higher-order visual thalamus during sustained attention.higher-order visual thalamus plays a fundamental but poorly understood role in attention-demanding tasks. to investigate how neuronal dynamics in higher-order visual thalamus are modulated by sustained attention, we performed multichannel electrophysiological recordings in the lateral posterior-pulvinar complex (lp/pulvinar) in the ferret (mustela putorius furo). we recorded single unit activity and local field potential (lfp) during the performance of the five-choice serial reaction time task ( ...201929619407
molecular detection of babesia spp. (apicomplexa: piroplasma) in free-ranging canids and mustelids from southern italy.babesiosis is an emerging tick-borne disease caused by apicomplexan parasites with widespread geographical distribution and various wildlife species as reservoir hosts. the aims of this study were to investigate the prevalence and assess the role of free-ranging canids and mustelids in the maintenance of babesia spp. in southern italy. pcr analysis of splenic samples targeting the 18s rrna gene revealed the presence of babesia spp. in 36 of 82 (43.9%) red foxes (vulpes vulpes) including 29 (58%) ...201931555669
reelin immunoreactivity in the adult spinal cord: a comparative study in rodents, carnivores, and non-human primates.reelin is a large extracellular matrix (ecm) glycoprotein secreted by several neuronal populations in a specific manner in both the developing and the adult central nervous system. the extent of reelin protein distribution and its functional role in the adult neocortex is well documented in different mammal models. however, its role in the adult spinal cord has not been well characterized and its distribution in the rodent spinal cord is fragmentary and has not been investigated in carnivores or ...201931969808
maternal immune activation alters adult behavior, gut microbiome and juvenile brain oscillations in ferrets.maternal immune activation (mia) has been identified as a causal factor in psychiatric disorders by epidemiological studies in humans and mechanistic studies in rodent models. addressing this gap in species between mice and human will accelerate the understanding of the role of mia in the etiology of psychiatric disorders. here, we provide the first study of mia in the ferret (mustela putorius furo), an animal model with a rich history of developmental investigations due to the similarities in d ...201930406186
molecular detection of anaplasma phagocytophilum in wild carnivores in north-eastern poland.anaplasma phagocytophilum is an obligate parasitic intracellular bacterium. it is the causative agent of granulocytic anaplasmosis, with effects on human and animal health. in europe, the pathogen is mainly transmitted among a wide range of vertebrate hosts by blood-sucking arthropods. the aim of this study was to determine the presence of a. phagocytophilum in wild carnivores, viz raccoon dogs (nyctereutes procyonoides), badgers (meles meles), foxes (vulpes vulpes), martens (martes sp.) and eur ...201931590678
helminths of mustelids with overlapping ecological niches: eurasian otter lutra lutra (linnaeus, 1758), american mink neovison vison schreber, 1777, and european polecat mustela putorius linnaeus, 1758.this study presents the helminthological data on three mustelid species with overlapping ecological niches in lithuania. in general, 14 helminth species or higher taxa were reported from all mustelids: isthmiophora melis, strigea strigis metacercariae, pseudamphistomum truncatum, alaria alata mesocercariae, phyllodistomum folium, opisthorchis felineus, metametorchis skrjabini, mesocestoides sp., taenia martis, aonchotheca putorii, crenosoma schachmatovae, eucoleus aerophilus, molineus patens, an ...201931662675
trichinella spp. in wild mesocarnivores in an endemic setting.human trichinellosis and trichinella infection in pigs are both still endemic in the balkans, including serbia. because of the flow between the sylvatic and the domestic cycle of trichinella spp., monitoring wildlife has been recommended for the risk assessment of trichinella spp. infection in swine. we have previously shown the presence of trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on trichinella infection in several other mesocarnivore ...201930922087
identification of mycobacterium genavense natural infection in a domestic ferret.a 6-y-old neutered male ferret ( mustela putorius furo) was presented because of a 1-mo history of progressive weight loss, chronic cough, and hair loss. on clinical examination, the animal was coughing, slightly depressed, moderately hypothermic, and had bilateral epiphora. thoracic radiography was suggestive of severe multinodular interstitial pneumonia. abdominal ultrasound examination revealed hepatosplenomegaly and mesenteric and pancreaticoduodenal lymphadenopathy. fine-needle aspiration o ...201930475677
distribution of gabaergic neurons and vglut1 and vgat immunoreactive boutons in the ferret (mustela putorius) piriform cortex and endopiriform nucleus. comparison with visual areas 17, 18 and 19.we studied the cellular organization of the piriform network [comprising the piriform cortex (pc) and endopiriform nucleus (ep)] of the ferret (mustela putorius)-a highly excitable region prone to seizures-and, more specifically, the distribution and morphology of different types of gamma-aminobutyric acid (gaba)ergic neurons, and the distribution and ratio of glutamatergic and gabaergic boutons, and we compared our findings to those in primary visual area 17, and secondary areas 18 and 19. we a ...201931213994
clinical and serologic remission of acquired myasthenia gravis in a domestic ferret (mustela putorius furo).a 4.5-year-old neutered male domestic ferret (mustela putorius furo) was examined because of clinical signs compatible with neuromuscular disease.201931039095
tick-borne pathogens in the european polecat, mustela putorius and in attached ixodes hexagonus ticks from germany.the european polecat, mustela putorius, occurs almost throughout europe. however, there is a lack of data on the ectoparasite fauna and the potential role in the circulation of tick-borne pathogens (tbp) of this mustelid species. the aim of this study was to investigate whether m. putorius contributes to the maintenance of tbp in germany. dna samples extracted from spleen tissue of 117 m. putorius, which had been collected mainly in north-western germany from 2012 to 2015, were tested by real-ti ...201930773320
highly efficient transgenesis in ferrets using crispr/cas9-mediated homology-independent insertion at the rosa26 locus.the domestic ferret (mustela putorius furo) has proven to be a useful species for modeling human genetic and infectious diseases of the lung and brain. however, biomedical research in ferrets has been hindered by the lack of rapid and cost-effective methods for genome engineering. here, we utilized crispr/cas9-mediated, homology-independent insertion at the rosa26 "safe harbor" locus in ferret zygotes and created transgenic animals expressing a dual-fluorescent cre-reporter system flanked by phi ...201930760763
cortical and thalamic connectivity of posterior parietal visual cortical areas ppc and ppr of the domestic ferret (mustela putorius furo).the present study describes the ipsilateral and contralateral cortico-cortical and cortico-thalamic connectivity of the parietal visual areas, posterior parietal caudal cortical area (ppc) and posterior parietal rostral cortical area (ppr), in the ferret using standard anatomical tract-tracing methods. the two divisions of posterior parietal cortex of the ferret are strongly interconnected, however area ppc shows stronger connectivity with the occipital and suprasylvian visual cortex, while area ...201930632607
cortical and thalamic connectivity of temporal visual cortical areas 20a and 20b of the domestic ferret (mustela putorius furo).the present study describes the ipsilateral and contralateral corticocortical and corticothalamic connectivity of the temporal visual areas 20a and 20b in the ferret using standard anatomical tract-tracing methods. the two temporal visual areas are strongly interconnected, but area 20a is primarily connected to the occipital visual areas, whereas area 20b maintains more widespread connections with the occipital, parietal and suprasylvian visual areas and the secondary auditory cortex. the callos ...201930632165
cortical and thalamic connectivity of occipital visual cortical areas 17, 18, 19, and 21 of the domestic ferret (mustela putorius furo).the present study describes the ipsilateral and contralateral corticocortical and corticothalamic connectivity of the occipital visual areas 17, 18, 19, and 21 in the ferret using standard anatomical tract-tracing methods. in line with previous studies of mammalian visual cortex connectivity, substantially more anterograde and retrograde label was present in the hemisphere ipsilateral to the injection site compared to the contralateral hemisphere. ipsilateral reciprocal connectivity was the stro ...201930632160
thelazia callipaeda in mustelids from romania with the european badger, meles meles, as a new host for this parasite.thelazia callipaeda (spirurida, thelaziidae) is a vector-borne zoonotic eye worm with a broad host spectrum. in europe, it is an emerging threat, having greatly expanded its geographical distribution during the past two decades. in romania, t. callipaeda has been previously reported in domestic and wild canids and felids. the aim of the present study was to assess the occurrence of t. callipaeda in mustelids in the country.201931349861
oral and dental examination findings in european polecats (mustela putorius).dental and oral diseases in the domestic ferret have been reported, but comparison with their closest wild relative, the european polecat (mustela putorius), is lacking. dental and orofacial pathology was evaluated by means of visual examination and dental radiographical analysis of 234 museum skulls. most of these (70.5%) originated from austria and the remainder came from seven other european countries. linguoverted mandibular second incisor teeth were noted in 77% of the skulls. there were 7, ...202032359635
rift valley fever virus infection causes acute encephalitis in the ferret.rift valley fever virus (rvfv) is a pathogen of both humans and livestock in africa and the middle east. severe human disease is associated with hepatitis and/or encephalitis. current pathogenesis studies rely on rodents and nonhuman primates, which have advantages and disadvantages. we evaluated disease progression in mustela putorius furo (the ferret) following intradermal (i.d.) or intranasal (i.n.) infection. infected ferrets developed hyperpyrexia, weight loss, lymphopenia, and hypoalbumine ...202033115835
putative modulation of the gut microbiome by probiotics enhances preference for novelty in a preliminary double-blind placebo-controlled study in ferrets.increasing evidence suggests a causal relationship between the gut microbiome and psychiatric illnesses. in particular, autism spectrum disorder is associated with gastrointestinal symptoms and alterations in the gut microbiome. administration of probiotics is a commonly used strategy by caregivers of people with neurodevelopmental illness. however, evidence for successful improvement in gut microbiome and (behavioral) symptoms has been lacking.202032490353
cardiopulmonary nematodes of wild carnivores from denmark: do they serve as reservoir hosts for infections in domestic animals?the cardiopulmonary nematodes angiostrongylus vasorum, crenosoma vulpis, capillaria aerophila and aelurostrongylus abstrusus, are a cause of concern in the scientific and veterinary community, potentially causing significant disease in domestic animals. to investigate the potential of wild carnivores as reservoir hosts to these parasites, a total of 1041 animals from seven regions of denmark were sampled: 476 raccoon dogs (nyctereutes procyonoides), 367 red foxes (vulpes vulpes), 123 american mi ...202032884901
tuberculosis in a pet ferret (mustela putorius furo).a 9-month old pet ferret was presented to a veterinarian with symptoms of weight loss, apathy, and hyporexia. explorative laparotomy identified a firm mass of approximately 2 × 2 × 2 cm in size in the mesentery of the jejunum. because of the poor general condition and the unfavorable prognosis, the ferret was euthanized during surgery. the mass was resected in total and submitted to histological examination which revealed a granulomatous and necrotizing lymphadenitis. acid fast bacteria were det ...202032059253
first draft genome of the sable, martes zibellina.members of genus martes provide early warning signals about forest ecosystem health and are designated as a management indicator species. as one of the most widespread members in martes, the sable (martes zibellina) is a circumboreal small predator found throughout all taiga zoogeographical zones of eurasia and shows distinct population differentiation and morphological variations. to support further studies on striking local adaptation and population evolution, we present the first sable genome ...202032058545
first report on natural infection with leishmania infantum in a domestic ferret (mustela putorius furo) in spain.a pet domestic ferret (mustela putorius furo) with a papular lesion involving the right pinna was diagnosed with chronic pyogranulomatous dermatitis by histopathologic examination. intralesional, intracytoplasmic oval microorganisms compatible with leishmania spp. or histoplasma spp. were observed in macrophages and multinucleate giant cells. leishmania infantum (l. infantum) infection was diagnosed by pcr, culture in novy-macneal-nicolle medium, and immunohistochemistry. abnormal clinicopatholo ...202032057396
utility of commercially available reagent test strips for estimation of blood urea nitrogen concentration and detection of azotemia in pet rabbits (oryctolagus cuniculus) and ferrets (mustela putorius furo).to evaluate the utility of commercially available reagent test strips for estimation of bun concentration and detection of azotemia in pet rabbits (oryctolagus cuniculus) and ferrets (mustela putorius furo).202031999516
proposed grading scheme for inflammatory bowel disease in ferrets and correlation with clinical signs.inflammatory bowel disease (ibd) is an idiopathic, chronic, inflammatory disease of the gastrointestinal tract of companion animals, including ferrets (mustela putorius furo). clinical signs of ibd are nonspecific, and intestinal biopsies are necessary for a definitive diagnosis. a grading scheme has not been established for ferrets. additionally, the association between histologic severity and clinical signs in ferrets is unknown. we evaluated enteric samples from ferrets diagnosed with ibd, co ...202031884894
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