Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [effect of loratadine--selective antagonist of histamine (h1) receptor--on allergen-induced bronchoconstriction in atopic asthmatics]. | the aim of this study was to evaluate the effect of loratadine on allergen-induced bronchoconstriction. the study was performed in 7 asthmatic patients with sensitivity to dermatophagoides pteronyssinus, 4 women and 3 men. aged from 19 to 37 years. the allergen inhalatory--challenge test was performed according to chai et al. early phase of dermatophagoides pteronyssinus--induced bronchoconstriction appeared in all examined patients, but late phase of bronchial obstruction was observed only in 3 ... | 1992 | 1303772 |
| monoclonal antibodies to lepidoglyphus destructor: delineation of crossreactivity between storage mites and house dust mites. | we have developed monoclonal antibodies (moabs) to the storage mite lepidoglyphus destructor (ld). employing these anti-ld moabs ld-moabs) in elisa and elisa inhibition techniques we have analysed the reaction pattern of ld-moabs to both non-pyroglyphid and pyroglyphid mites. the storage mite glycyphagus domesticus (gd) exhibited most efficient inhibition, followed by acarus siro (as), tyrophagus putrescentiae (tp), dermatophagoides pteronyssinus (dpt) and euroglyphus maynei (em). of the two pyr ... | 1992 | 1468029 |
| soy hypersensitivity in children with food allergy. | to evaluate humoral (ige antibodies) and clinical (positive challenge test) soy hypersensitivity prevalence, we studied 317 children (271 boys and 100 girls) with a median age of 5 months (range 1-120) who visited the division of allergy and clinical immunology of the pediatric department of the university of roma "la sapienza" because of histories and symptoms suggestive of food allergy. atopic dermatitis (ad) was present in 247/317 children (78%), diarrhea in 19 (6%), urticaria in 22 (7%), and ... | 1992 | 1380784 |
| analysis of antigenic determinants shared by two different allergens recognized by human t cells: house dust mite (dermatophagoides pteronyssinus) and chironomid midge (chironomus yoshimatsui). | to analyse the cross-reactivity of t-cell-mediated immunity between dermatophagoides pteronyssinus (dp) and chironomus yoshimatsui (cy), the most common allergens in japan, we established antigen-specific human t-cell lines and clones. some but not all of the cy-induced t-cell lines showed a significant proliferative response not only to cy, but also to dp. no t-cell line responded to other unrelated antigens. when we stimulated the dp-induced t-cell clones with cy, 3 of the 40 clones (7.5%) sho ... | 1992 | 1283062 |
| dust from carpeted and smooth floors. v. cat (fel d i) and mite (der p i and der f i) allergen levels in school dust. demonstration of the basophil histamine release induced by dust from classrooms. | sixty dust samples from schools in norway were analysed for major allergens from cat and mite after sampling with the regularly used vacuum cleaners for 5 days and with a new model vacuum cleaner for 10 days, respectively. the major feline allergen felis domesticus allergen i (fel d i) was detected in all the classrooms, with ranges from 12 to 16,840 ng/m2 floor area. the mean fel d i concentration was about 11 times higher per unit area carpeted floors as compared with smooth floors after the 1 ... | 1992 | 1283110 |
| allergic skin reactivity in 232 patients over 50 years. | we examined two hundred and thirty-two out-patients, ranging in age from 50 to 75 yrs. all patients were submitted to skin-tests: prick and, if it was necessary, intradermal reactions and/or rast. allergens employed were gramineae, parietaria, olea, dermatophagoides farinae, dermatophagoides pteronyssinus, house dust, cat and dog skin scales. one hundred and twenty-eight subjects had positive responses to one or more aeroallergens. asthma was the most frequent condition both in skin-positives an ... | 1992 | 1292328 |
| plasma kallikrein amidolytic activity in bronchial asthma. | we have investigated plasma kallikrein amidolytic activity in the following groups of patients: 1. normal control group of blood donors. 2. extrinsic pollen-activated bronchial asthma patients, during periods of symptomatology and at a later time after the spring. 3. subjects with atopic bronchial asthma in acute phase when admitted to our hospital's emergency room and later when clinically recovered. 4. subjects with extrinsic bronchial asthma, sensitive to dermatophagoides pteronyssinus and de ... | 1992 | 1292330 |
| skin sensitivity patterns to inhalant allergens in nigerian asthmatic patients. | the pattern of skin sensitivity response to inhalant allergens in 746 nigerian asthmatics and 92 normal controls tested over 10 years period is reported. significant differences in reactions were observed in 7 of the 16 allergens routinely used in the study. house dust mite--dermatophagoides pteronyssinus gave the highest incidence of positive skin reaction, 58% asthmatics, 4.3% controls (p < 0.001). other significant allergens were house dust 51.7%, feathers 24.4%; dog hair 12.9%, cat fur 11.9% ... | 1992 | 1298621 |
| biological characterization of a formulated allergoid from dermatophagoides pteronyssinus. | a comparative study between the biological properties of the allergen dermatophagoides pteronyssinus (dp) and its formulated allergoid (dphcho) was performed. after immunizing rats with dp or dphcho the serum level time course of ige and igg was determined by passive cutaneous anaphylaxis (pca) and indirect-elisa, respectively. the avidity for ige (pca and pca-inhibition) or igg (indirect elisa and elisa inhibition) and the ability to induce anaphylactic shock were also determined. serum ige pat ... | 1992 | 1302894 |
| t cell activation-inducing epitopes of the house dust mite allergen der p i. proliferation and lymphokine production patterns by der p i-specific cd4+ t cell clones. | cloned human cd4+ t cell lines specific for the house dust mite dermatophagoides pteronyssinus were used to map minimal t cell activation-inducing epitopes on the group i allergen in d. pteronyssinus extracts (der p i) molecule. most of these der p i-specific t cell clones expressed different tcr v alpha and v beta gene products. using recombinant deletion proteins, three t cell epitopes were identified on the der p i molecule; p45-67 and p117-143 were recognized by hla-dr7-restricted t cells, w ... | 1992 | 1370514 |
| identification of allergenic epitopes on der f i, a major allergen of dermatophagoides farinae, using monoclonal antibodies. | the antigenic and allergenic structure of der f i, a major allergen of the house dust mite dermatophagoides farinae (df) was investigated by means of a panel of 11 selected monoclonal antibodies (mab) obtained from balb/c mice immunized with purified der f i. the species specificity of these mab, tested with der f i and der p i--the homologous allergen from dermatophagoides pteronyssinus--was generally restricted to der f i since 10 out of 11 mab reacted only with this allergen. epitope specific ... | 1992 | 1371821 |
| house dust mite-specific t cells in the skin of subjects with atopic dermatitis: frequency and lymphokine profile in the allergen patch test. | the mechanism underlying positive patch tests with house dust mite-allergen, dermatophagoides pteronyssinus (der p), in patients with atopic dermatitis was investigated by isolating t cells from the test sites of two patients. eighty-five t cell clones (tcc) were established from the epidermis and dermis of lesional skin by the limiting-dilution method with der p and interleukin (il)-2. with restimulation assays, 29 of 60 tccs tested demonstrated specific proliferation; 85% were of the cd3+, cd2 ... | 1992 | 1373161 |
| abnormal humoral immune response to mucosal antigenic stimulation in patients with lung cancer. | previous studies have suggested an inverse relationship between atopy and cancer of mucosal surfaces. atopy is classically assessed by detecting specific immunoglobulin e (ige) antibodies against inhalant allergens. however, platts-mills recently proposed that atopy is the ability of an organism to recognize and to respond to limited doses of allergens presented at the mucosal level by producing not only ige, but also immunoglobulin a and immunoglobulin g (igg) antibodies. the authors compared t ... | 1992 | 1373339 |
| localization of allergens in the domestic mite lepidoglyphus destructor. | the non-pyroglyphid domestic mite lepidoglyphus destructor is a major source of allergen causing respiratory symptoms in farming environments. this study is the first to focus on the localization of the allergens in the non-pyroglyphid domestic mite lepidoglyphus destructor. cryostat-cut sections of l. destructor mite bodies and faecal pellets were probed with one of three mouse monoclonal antibodies (moabs) raised against l. destructor or with patient sera, and stained with immunoperoxidase. ei ... | 1992 | 1373986 |
| specific histamine release capacity of peptides selected from the modelized der p i protein, a major allergen of dermatophagoides pteronyssinus. | dust mite allergens are considered as a major cause of allergic disease and as a risk factor for asthma. der p i, a 222 amino-acid residue globular glycoprotein, is one of the major allergens from dermatophagoides pteronyssinus (dpt) mites. in this study, we have used predictive conventional algorithms (i.e. hydrophilicity, mobility, accessibility) and a three-dimensional model of der p i derived from comparison to actinidin and papain to select continuous amino acid sequences as potential b cel ... | 1992 | 1376413 |
| peptide-induced nonresponsiveness of hla-dp restricted human t cells reactive with dermatophagoides spp. (house dust mite). | the activation of cd4+ t lymphocytes, which play a central role in allergic inflammation, depends on the recognition of allergen-derived peptides in association with major histocompatibility complex class ii gene products. in this report we demonstrate, at a clonal level, that a component of the t-cell repertoire reactive with dermatophagoides spp. (house dust mite) in atopic individuals, is restricted by hla-dp class ii molecules. this supports the recent results emerging from genetic epidemiol ... | 1992 | 1385507 |
| specific activation of platelets from patients allergic to dermatophagoides pteronyssinus by synthetic peptides derived from the allergen der p i. | previous studies have shown that fc epsilon rii-bearing platelets from patients with allergic disorders could be stimulated either by anti-human ige antibodies or the relevant allergens to generate cytotoxic mediators. in this report, the reactivity of platelets from dermatophagoides pteronyssinus (d. pt)-sensitive patients to three der p i-derived synthetic peptides (52-71, 117-133 and 188-199) was investigated. the platelet stimulation observed in d. pt-sensitive patients was demonstrated to b ... | 1992 | 1386546 |
| comparative efficacy of house dust mite extermination products. | the acaricidal efficacy of nine marketed products, i.e. acardust, acarosan (foam and powder), actelic 50, artilin 3a (spirit and water base), liquid nitrogen, paragerm ak, and tymasil, and of intensive vacuum-cleaning have been compared on four different test surfaces: mattress, tufted carpet, gypsum board and rough wooden board, all covered with artificial house dust. they were inoculated with the house dust mite, dermatophagoides pteronyssinus or the house-dust fungus aspergillus repens for ev ... | 1992 | 1393759 |
| the allergen specific b-cell response during immunotherapy. | the paper examines the allergen specific b-cell response in peripheral blood from patients undergoing immunotherapy with house dust mite extract. the 12 patients were part of a double blind placebo controlled study, and they were treated with either dermatophagoides pteronyssinus (n = 4), dermatophagoides farinae extract (n = 3) (alutard sq, alk, denmark) or placebo (n = 5). blood was taken every fortnight on day seven after hyposensitization and tested for igm, igg, iga and ige antibody secreti ... | 1992 | 1393764 |
| longitudinal variability of skin prick test results. | the skin prick test (spt) is a commonly used procedure for assessing a specific sensitization. the longitudinal variability of test results is of interest for clinical as well as epidemiological investigations. the sensitization to four common aeroallergens (grass pollen, birch pollen, dermatophagoides pteronyssinus, cat dander) is investigated within the framework of three consecutive spts at 11-month intervals for a population of 587 schoolchildren. the prevalence of sensitization based on a w ... | 1992 | 1422941 |
| skin sensitivity patterns to inhalant allergens in nigerian asthmatic patients. | the pattern of skin sensitivity response to inhalant allergens in 746 nigerian asthmatics and 92 normal controls tested over 10 years period is reported. significant differences in reactions were observed in seven of the 16 allergens routinely used in the study. house dust mite--dermatophagoides pteronyssinus--gave the highest incidence of positive skin reaction in 58 pc asthmatics and 4.3 pc controls (p = 0.002). other significant allergens were house dust, 51.7 pc, feathers; 24.4 pc, dog hair, ... | 1992 | 1423548 |
| longitudinal changes in skin-prick test reactivity over 2 years in a population of schoolchildren with respiratory symptoms. | as part of a larger epidemiological study, 114 children with respiratory symptoms, born between 1978 and 1980, were skin-prick tested to dermatophagoides pteronyssinus (dp), mixed grass pollens (g) and cat dander (c), and to histamine and saline controls (bencard, u.k.) using 1 mm prick-lancets (dome/hollister-stier), between july and september 1987 and again in october 1989. a weal > or = 2 mm to one or more allergens was regarded as a positive result. each child was tested by the same investig ... | 1992 | 1464050 |
| respiratory allergies (bronchial asthma and rhinitis) due to sensitization of type i allergy to red spider mite (panonychus ulmi koch). | the inhabitants of a fruit growing area often report spontaneously of sensitization to the red spider mite (rsm) (panonychus ulmi koch). these are for the most part sensitizations with low clinical symptoms (rhinitis, conjunctivitis and erythema). severe clinical developments with bronchial asthma have been observed. we investigated six patients working in a fruit growing area sensitized by rsm. the sensitizations corresponded to a type i allergy. skin tests and provocation tests (nasal as well ... | 1992 | 1464051 |
| immunopathology of sids. | immunopathological studies of sids share the problems of all necropsy based studies of this syndrome: the extent of autolytic changes in the material under study; and the lack of appropriate controls. despite these problems, several studies have been performed on serum, bronchoalveolar lavage, and pulmonary tissue. many of these studies have been inspired by the modified anaphylaxis hypothesis, based on the experiments of coombs and coworkers. lightly anaesthetised guinea-pigs, which had been se ... | 1992 | 1474158 |
| how relevant are house dust mite-fungal interactions in laboratory culture to the natural dust system? | both house dust and house dust mites dermatophagoides pteronyssinus contained a wider range of fungi than laboratory mite cultures. in total, nine species of fungi were isolated from d. pteronyssinus in house dust, and these included three xerophilic species (eurotium amstelodami, aspergillus penicillioides and wallemia sebi) commonly found in laboratory cultures of d. pteronyssinus. it is concluded that mites do interact with a similar range of fungi in natural dust and in laboratory culture, b ... | 1992 | 1493747 |
| responsiveness to histamine in human sensitized airway smooth muscle. | passive sensitization of human isolated airway smooth muscle increases contractile responses to histamine. we looked to see whether this increase was due to an alteration in the relative role of histamine h1 and h2 receptors. human bronchial spiral strips obtained at thoracotomy were passively sensitized by incubation in serum from atopic asthmatic patients to dermatophagoïdes pteronyssinus and control strips were incubated in serum from healthy non-allergic non-atopic subjects. we also studied ... | 1992 | 1494723 |
| major histocompatibility complex (mhc) class ii restriction, lymphokine production, and ige regulation of house dust mite-specific t-cell clones. | to elucidate the regulatory mechanism of human ige synthesis, we have cloned house dust mite (dermatophagoides pteronyssinus; dp)-specific t-cell clones from three asthmatic children and three healthy individuals. twelve clones were cloned from each group. all of these clones were cd3+, cd4+, cd8-, and hla-dr+. after stimulation with allergen in the presence of antigen presenting cells (apcs), half of the t-cell clones from asthmatic children and one-third of those from normals produced interleu ... | 1992 | 1512301 |
| [bronchial asthma in children with sensitization to mites]. | overall 180 children with bronchial asthma induced by sensitization to dermatophagoides pteronyssinus and dermatophagoides farinae were examined clinically and immunologically. the patients' age ranged from 1 to 14 years. a relationship was established between bronchial asthma exacerbation and the stay at home. in that disease pattern, asphyxia attacks were noted to occur more frequently in the evening and night hours. emphasis is laid on the leading part played by the ige-mediated mechanism in ... | 1992 | 1513646 |
| early childhood risk factors for sensitization at school age. | early childhood risk factors for current sensitization were investigated by use of cross-sectional data of a longitudinal study in southwest germany. information was gathered by questionnaires from 1812 families of whom 1470 children 6 to 8 years old were tested by means of a skin prick test (spt) with seven aeroallergens. groups with sensitization (n = 201; positive spt to grass pollens 6.6%, dermatophagoides pteronyssinus 6.5%, dermatophagoides farinae 4.4%, cat dander 4.6%, any of the tested ... | 1992 | 1527318 |
| reciprocal regulatory effects of ifn-gamma and il-4 on the in vitro development of human th1 and th2 clones. | the effects exerted on the in vitro development of purified protein derivative (ppd)-specific or dermatophagoides pteronyssinus group i (der p i)-specific t cell lines (tcl) and t cell clones (tcc) by il-4 or ifn-gamma addition or neutralization in human pbmc cultures were examined. pbmc from two normal individuals, which were stimulated with ppd and then cultured in il-2 alone, developed into ppd-specific tcl and tcc able to produce ifn-gamma and il-2 but not il-4 and il-5 (th1-like). ifn-gamma ... | 1992 | 1532000 |
| comparison of allergic responses to dust mites in u.k. bakery workers and swedish farmers. | the ige rast response to dermatophagoides pteronyssinus and four storage mites (lepidoglyphus destructor, tyrophagus putrescentiae, glycyphaghus domesticus, and acarus siro) was examined in 251 u.k. bakery workers and compared with that previously found in 440 swedish farmers. storage mites are found commonly in stored hay and grain so both these groups potentially encounter them in their work. in neither group of workers was a positive rast (greater than or equal to 0.35 pru) to d. pteronyssinu ... | 1992 | 1571817 |
| evidence refuting the contribution of the fungus aspergillus penicillioides to the allergenicity of the house dust mite dermatophagoides pteronyssinus. | this communication demonstrates unequivocally that the fungi associated with house dust mites do not contribute to mite allergenicity. the evidence is twofold: first, larval mites which lack fungi have allergen profiles indistinguishable from fungus-bearing adult mites. second, the allergen profile of experimentally-derived fungus-free adult mites and mites re-fed the fungus aspergillus penicillioides are identical. | 1992 | 1582703 |
| comparison between magic lite- and cap-system: two automated specific ige antibody assays. | the magic lite system, a newly developed immunochemiluminometric assay for specific and total ige antibody using paramagnetic particles coupled with standardized allergens as solid phase, was compared to the cap system, a recently introduced immunoassay based on a cellulose polymer encased in a capsule. a total of 357 serum samples of patients with suspected inhalant allergies and a positive skin prick test (spt) to common allergens (birch, timothy-grass, mugwort, cat dander, dermatophagoides pt ... | 1992 | 1611547 |
| the house dust mite dermatophagoides pteronyssinus is the most important allergen on the island of mauritius. | to determine the relative importance of mites as a cause of allergic sensitivity and asthma on the western indian ocean island of mauritius, we measured specific ige antibodies to common inhalant allergens in sera from mauritians claiming to have allergic symptoms and we examined house dust samples for evidence of mites and their allergens. seventy-two of the 110 sera tested (65%) contained detectable ige antibody to at least one mite, mould or pollen allergenic extract. by far the most prevalen ... | 1992 | 1628251 |
| reduction of environmental mites improved atopic dermatitis patients with positive mite-patch tests. | during a series of studies on the involvement of house dust mite antigens in 183 cases of atopic dermatitis, we observed an improvement in two patients following the removal of mites from their environment by means of a thorough housecleaning and replacement of the mattress. both patients manifested the typical clinical skin lesions of atopic dermatitis and had similar laboratory findings. although the serum ige concentrations and specific ige to dermatophagoides pteronyssinus and dermatophagoid ... | 1992 | 1640023 |
| clonal analysis of the atopic immune response to the group 2 allergen of dermatophagoides spp.: identification of hla-dr and -dq restricted t cell epitopes. | the group 2 allergens of dermatophagoides spp. (house dust mite, hdm) are a major immunological target for ige antibodies in the allergic immune response of hdm atopic individuals. in this report the heterogeneity of the t cell repertoire reactive with the group 2 allergen of dermatophagoides pteronyssinus (der p 2) of a hdm allergic individual was investigated using overlapping synthetic peptides. by clonal analysis four distinct t cell epitopes were identified, located within residues 16-31, 2 ... | 1993 | 7508743 |
| a study of the binding of immunoglobulin g and immunoglobulin e from children with bronchial asthma to peptides derived from group ii antigen of dermatophagoides pteronyssinus. | the peptides that were produced by the treatment of the purified group ii antigen of dermatophagoides pteronyssinus with cyanogen bromide, lysylendopeptidase, staphylococcus aureus v8 protease, or n-tosyl-l-phenylalanylchloromethyl ketone-treated trypsin were examined for their binding with igg and ige from children with bronchial asthma by immunoblotting analysis. both igg and ige bound to the peptides from the amino terminus to the 76th residue and the carboxyl terminal side from the 13th or 1 ... | 1993 | 7679489 |
| comparative ige responses to extracts of five species of house dust mite, using western blotting. | ige responses towards extracts of dermatophagoides pteronyssinus, d. farinae, d. microceras, euroglyphus maynei and gymnoglyphus longior have been evaluated with sera from a mite sensitive population and the international reference pool of sera, nibsc 82/528, using western blotting. using mite body and faecal extracts 21 of 29 mite protein bands responded to by sera were common to all five species of mite. all sera were unique with respect to proteins bound and species recognized. mite proteins ... | 1993 | 7680599 |
| comparative analysis of biological activities of der p i-derived peptides on fc epsilon receptor-bearing cells from dermatophagoides pteronyssinus-sensitive patients. | the ability of four uncoupled synthetic peptides (p52-71, p117-133, p176-187, p188-199) derived from der p i, a major allergen from the house dust mite dermatophagoides pteronyssinus (dpt) to stimulate fc epsilon r+ cells from dpt-sensitive patients was comparatively analysed. each free peptide may specifically stimulate basophils (fc epsilon ri+ cells) and platelets (fc epsilon rii+ cells) from patients with significant levels of anti-der p i ige antibodies; p52-71 and p117-133 appear the best ... | 1993 | 7682161 |
| effect of salbutamol, fenoterol, and sodium cromoglycate on the release of heparin from sensitized human lung fragments challenged with dermatophagoides pteronyssinus allergen. | heparin from degranulating mast cells influences a wide range of cellular and humoral reactions associated with allergic inflammation and asthma. agents that inhibit mast cell degranulation may therefore compromise the moderating effects of heparin in the tissues and result in worsening inflammation and other associated pathology. this study measures heparin release from allergen-challenged human lung tissue and compares the effect of the mast cell stabilizing beta 2-agonists, salbutamol and fen ... | 1993 | 7683196 |
| effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by substance p, neurokinin a and vip. | 1. the effect of passive sensitization on the mechanical activity of human isolated bronchial smooth muscle induced by the following neuropeptides substance p (sp), neurokinin a (nka) and vasoactive intestinal peptide (vip) was studied both in the absence and in the presence of the neutral endopeptidase (nep) inhibitor, phosphoramidon. 2. cumulative concentration-response curves (ccrc) to these neuropeptides were constructed in human passively sensitized isolated bronchial rings and compared to ... | 1993 | 7684296 |
| t cell epitopes of house dust mite major allergen der p ii. | recent work has indicated the significance of il-4- and il-5-secreting allergen-specific human th2 lymphocytes in the control of immune responses to allergens in atopic individuals. the precise allergenic epitopes that activate these allergen-specific th2 cells are, however, hardly known. we analyzed the epitope-specificity of t lymphocytes specific for der p ii, one of the major allergens of house dust mite dermatophagoides pteronyssinus. using a panel of overlapping synthetic peptides that spa ... | 1993 | 7688399 |
| human in vitro experimental model for cd4+ t cell targeted immunotherapy to house dust mite. | cd4+ t cells play a critical role in the initiation and potentiation of the allergic immune response. development of allergen-mediated hyposensitization therapies directed at this cell type requires two key steps: (1) identification of the epitopes that are crucial for activating the t-cell response and (2) definition of the specificity of the hla-d region molecules that restrict t-cell recognition of these epitopes. we have located the major t-cell determinants of the group ii allergen of the h ... | 1993 | 7690528 |
| mite fauna, der p i, der f i and blomia tropicalis allergen levels in a tropical environment. | fifty dust samples were collected from the mattresses and bedroom floors of 25 subjects with allergic asthma in cartagena, colombia, in order to identify house dust mites and quantitate der p i, der f i and blomia tropicalis allergens. the geometric mean of the total mite density per gram of dust was 418 (range, 40-2280). twenty-two samples (44%) had more than 500 mites and four, less than 100. b. tropicalis and dermatophagoides pteronyssinus were found in 96% and 90% of the samples, accounting ... | 1993 | 8319126 |
| evaluation of allergen-specific ige antibodies by mast for the diagnosis of nasal allergy. | multiple-antigen simultaneous test (mast) is a new system for detecting allergen-specific ige antibodies. multiple antigens can be examined simultaneously in a short period of time by this method. the purpose of this study is to evaluate the usefulness of this method and to compare the results of mast with those of rast and intradermal skin test using 133 serum samples obtained from patients with nasal allergy. the positive rates of the main allergens detected by the mast system are 56% for japa ... | 1993 | 8321979 |
| screening for ige mediated allergy among people working in the marseilles harbour. | screening for ige mediated allergy by rasts to professional (castor bean, green coffee, peanut, soy protein, wheat, rice), and non professional (pollens, mites, cat, alternaria tenuis) air borne antigens among 36 people working in the marseilles harbour has showed rather unexpected findings: only one case of ige positivity to dermatophagoides pteronyssinus (class i) and one case of ige positivity to castor bean seed (ricinus communis) (class iv). igg4 specific antibodies against castor bean and ... | 1993 | 8328353 |
| allergic skin tests and respiratory diseases in 1612 subjects. | in 1985-90, 1612 subjects were submitted to allergic tests for respiratory diseases. in a retrospective survey we have evaluated clinical features, symptoms, diagnostic procedure and allergic tests of skin positives and skin negatives. prick tests were supplemented by intradermal reaction and rast under guideline of history. allergens examined were gramineae, parietaria, olea, house dust, dermatophagoides farinae and pteronyssinus. 80% of patients were found positive to one or more allergens; th ... | 1993 | 8328355 |
| analysis of human t cell responses to the group ii allergen of dermatophagoides species: localization of major antigenic sites. | ige antibodies reactive with the group ii allergens of dermatophagoides species (house dust mite [hdm]) are a major component of the allergic immune response in hdm-allergic atopic individuals. | 1993 | 8335846 |
| igg and ige antibody responses to dust mite allergens among children with asthma in brazil. | we compared exposure and levels of ige and igg antibodies to inhalant allergens in 20 children with asthma and positive skin tests to dermatophagoides pteronyssinus, living in são paulo, to 20 control children without history of asthma or allergy and negative skin tests to d. pteronyssinus, d. farinae, and blomia tropicalis. in 16/20 and 17/20 houses of asthmatic and control children, respectively, at least one sample was obtained which contained > 10 micrograms der p i/g of dust. serum ige anti ... | 1993 | 8346869 |
| influence of low temperature (-30 degrees c) on the different stages of the human allergy mite dermatophagoides pteronyssinus (acari: epidermoptidae). | effects of low temperature (-30 degrees c) on the different life stages of dermatophagoides pteronyssinus (trouessart) were studied. humid cold air at -30 degrees c killed all stages of mites except eggs after 10 min; after 35 min exposure, 20% of the eggs hatched after the incubation period. dry, cold air at -30 degrees c killed 40% of all stages of mites except eggs after 4 min and 100% after 5 min; all eggs died after 1 min exposure. | 1993 | 8360908 |
| effects of the fungus aspergillus penicillioides on the house dust mite dermatophagoides pteronyssinus: an experimental re-evaluation. | in this report the widely-held view that house dust mites benefit from fungal contamination of the dietary substratum is re-examined. the performance of dermatophagoides pteronyssinus (acari: pyroglyphidae) is documented over two successive generations in the presence or absence of the xerophilic fungus aspergillus penicillioides (hyphomycetales: moniliaceae). this fungus reduced survival, development rate, adult length and fecundity of d. pteronyssinus. detrimental effects of a. penicillioides ... | 1993 | 8369562 |
| [a comparative study on skin tests and allergen-specific ige levels determined by enzyme allergo-sorbent testing (east)]. | ninety seven randomly selected subjects with bronchial asthma and allergic rhinitis were classified for the comparative studies. the level of allergen specific ige determined by enzyme allergo-sorbent testing (east) was compared with skin tests. the statistically significant correlation was found for 9 selected allergens. however, the spearman's rank correlation coefficient values varied largely, depending on a given allergen. they ranged from 0.74 (dermatophagoides pteronyssinus) to 0.33 (birch ... | 1993 | 8401337 |
| relationship between exposure to dust mite allergen and bronchial response to exercise in schoolchildren sensitized to dust mites. | we assessed the relationship between the exposure to dust mite allergens and a bronchial response to exercise in 8-year-old schoolchildren. dust was collected from the mattresses of 1,291 children and the concentration of mite allergens was estimated by a commercially available elisa test using monoclonal antibodies (alk, copenhagen) against the major allergens of dermatophagoides pteronyssinus (der pt) and dermatophagoides farinae (der f). a positive bronchial response to exercise (decrease of ... | 1993 | 8414735 |
| antibody affinity disturbance in the immunoglobulin immune response to mucosal antigenic stimulation in lung cancer. the betalactoglobulin model. | this study is a continuation of a recent study, in which a defect in the immunoglobulin g (igg) response to some natural antigens (bovine betalactoglobulin [blg] from cow's milk and antigen p1 from the house dust mite dermatophagoïdes pteronyssinus), usually presented at the mucosal level, was documented in lung cancer patients. the present study further characterizes this difference in terms of antibody relative functional affinity in the blg model. | 1993 | 8422625 |
| sensitization to chortoglyphus arcuatus and aleuroglyphus ovatus in dermatophagoides spp. allergic individuals. | the prevalence of specific ige to the storage mites aleuroglyphus ovatus (ao) and chortoglyphus arcuatus (ca) was studied in 77 individuals with allergic asthma and/or chronic allergic rhinitis. all these individuals had a positive skin test (weal > or = 3 mm) to extracts of dermatophagoides pteronyssinus (dp) and/or dermatophagoides farinae (df). sera from 29 non-atopic individuals were used as controls. a rast was considered positive when a serum bound > or = 1% of the total counts added. the ... | 1993 | 8448678 |
| serum-ige-facilitated allergen presentation in atopic disease. | the occurrence of facilitated ag presentation (fap) was investigated in atopic disease using an in vitro model in which the ability of cd23-expressing ebv-b cells was tested to present der p ii, a major allergen of housedust mite dermatophagoides pteronyssinus, to cells of autologous der p ii-specific cd4+ t lymphocyte clones. purified der p ii protein was immune complexed with ig by preincubation in sera from atopic patients containing der p ii-specific ige. incubation of ebv-b cells with these ... | 1993 | 8468493 |
| the effects of season, climate, and air-conditioning on the prevalence of dermatophagoides mite allergens in household dust. | clinical evidence reveals a strong relationship between dust mite allergen levels and asthma. this study suggests the relative importance and interactions among factors that influence mite allergen levels in human dwellings. | 1993 | 8473674 |
| allergenicity of the mite, blomia tropicalis. | blomia tropicalis (bt) occurs in a significant percentage of homes in tropical and subtropical geographic regions of the united states and europe and in countries in south america and asia along with the pyroglyphid mites, euroglyphus maynei (em), dermatophagoides pteronyssinus, and d. farinae. blomia species may be major sources of allergens in house dust in addition to dermatophagoides species and e. maynei. | 1993 | 8491936 |
| clonal analysis of human t-cell responses to fractionated house dust mite antigens (dermatophagoides pteronyssinus). | in the present study, we investigated the cellular basis of house dust mite-driven immune responsiveness in an atopic individual with perennial rhinitis. we established 40 human t-cell clones (cd3+, 4+, 8-) reactive to dermatophagoides pteronyssinus (dp) antigen under the restriction of hla-dr. by using the crude dp antigen and its 14 molecular weight (mw) fractions, we analyzed the distribution of t-cell-recognizing sites in the whole dp extract. we tested t-cell-mediated immunity through two p ... | 1993 | 8499778 |
| patch test reactions to mite antigens: a gerda multicentre study. groupe d'etudes et de recherches en dermato-allergie. | we performed patch tests with dermatophagoides pteronyssinus (dp) antigens from 2 different sources in 355 non-randomly selected patients with atopic dermatitis (ad) and 398 subjects of a control group. the study demonstrated that contact sensitization to mites occurred in an appreciable % of ad cases (20.8%), using commonly available assay products. the differences recorded between the 2 materials tested were related to the concentration of p1 antigen. non-atopic patients rarely showed positive ... | 1993 | 8112063 |
| sensitization to four common inhalant allergens within 302 nuclear families. | the coincidence of allergic sensitization was investigated in 302 school-aged children and their parents. specific sensitization to four common inhalant allergens (grass and birch pollens, cat dander, dermatophagoides pteronyssinus) was ascertained by means of skin-prick tests (spt) carried out on the complete family unit at the beginning of a 22-month follow-up period. the same test procedure was then repeated on the children twice at 11-month intervals to provide cumulative prevalences of sens ... | 1993 | 8221260 |
| cd23 expression on b-lymphocytes and its modulation by cytokines in allergic patients. | the aim of this study was to assess the expression of cd23 on peripheral blood b-cells, and its in vitro modulation by recombinant human interferon-gamma (ifn-gamma) in phytohaemagglutinin-(pha) or recombinant human interleukin-4 (il-4)-stimulated cultures in atopic patients with dermatophagoides pteronyssinus hypersensitivity and in healthy non-atopic subjects. atopic patients with asthma not receiving allergen-specific immunotherapy (n = 21) were studied and further compared with a group of at ... | 1993 | 8221262 |
| characterization and partial purification of the croatian national standard dermatophagoides pteronyssinus allergen extract. | lyophilized dermatophagoides pteronyssinus (der p) allergen extract (ae) and partially purified der p extract (pae) were prepared and characterized. partial purification of ae was performed by gel filtration on sephadex g-100 and sephacryl s-300. crossed immunoelectrophoresis (cie) disclosed the same precipitating lines in ae and pae preparations. the relative potencies of ae and pae were determined and compared with the who international standard for der p by the rast inhibition method. the pot ... | 1993 | 8238802 |
| a different profile of epitopic dominance in the immunoglobulin g response to bovine betalactoglobulin in lung cancer. | the authors previously documented a quantitative defect in the immunoglobulin g (igg) response toward bovine betalactoglobulin (blg), the major cow's milk antigen, and antigen p1 of the house dust mite, dermatophagoides pteronyssinus (der p1), in patients with lung cancer. in the der p1 model, the authors documented at the igg level an epitope specificity that differed between patients with lung cancer (preferential specificity for cryptic epitopes) and healthy control subjects and patients with ... | 1993 | 8252475 |
| study of the sensitization to storage mites in a pediatric population in barcelona. | we performed the following study in order to determine the prevalence and characteristics of sensitization to storage mites in children mostly from the city of barcelona. all children over 3 years of age attending our unit for the first time due to respiratory problems were given cutaneous tests using the prick test for the house dust mites dermatophagoides pteronyssinus and d. farinae, and the storage mites acarus siro, lepidoglyphus destructor and tyrophagus putrescentiae. we carried out a ras ... | 1993 | 8281346 |
| mite allergy in the tropics: sensitization to six domestic mite species in cartagena, colombia. | sensitization to the domestic mites blomia tropicalis, chortoglyphus arcuatus, lepidoglyphus destructor and aleuroglyphus ovatus was investigated in 77 subjects with clinical symptoms of asthma and/or allergic rhinitis and a positive skin prick test (wheal > or = 3 mm) to dermatophagoides pteronyssinus and/or d. farinae. seventy patients were skin test-positive to d. pteronyssinus and 73 to d. farinae. serum samples from 29 nonallergic, skin test-negative individuals were used as negative contro ... | 1993 | 8281353 |
| prevalence of patent and latent atopy among a general normal adult population in the south east of france by rast investigation and correlation with circulating total ige levels. | a population of 512 adults (men: 266, 52%, women: 246, 48%) with mean age 41.4 years (s.d. 10.7) was investigated. this population was subdivided into five groups: marseilles city, manosque a small town in a prealpine area, the area surrounding the etang de berre, a rural area in northern bouches du rhône and chateaurenard, a small town in an intensive agricultural area. rasts to a panel of eight allergens (lolium perenne, parietaria officinalis, cupressus sempervirens, olea europea, cat dander, ... | 1993 | 8160568 |
| molecular cloning of a house dust mite allergen with common antibody binding specificities with multiple components in mite extracts. | plaque radio-immuno assay has been used to isolate an ige-binding clone from a lambda gt11 library of dermatophagoides pteronyssinus cdna. the clone hd6 contained dna encoding a 215 residue protein which contained a predicted 17 amino acid residue leader sequence, no cysteines and a single n-glycosylation site. the 198 residue mature protein would have a predicted mw of 22,177 d. no homologues were found in searches of the data banks. sera from 14/38 allergic children reacted strongly with the p ... | 1993 | 10779281 |
| humoral response to subcutaneous, oral, and nasal immunotherapy for allergic rhinitis due to dermatophagoides pteronyssinus. | we investigated variations in total ige, and specific ige, igg, and igg4 for dermatophagoides pteronyssinus in 57 patients with perennial rhinitis due to house dust mite allergy in an open controlled study: 43 subjects received immunotherapy (17 subcutaneous, 14 sublingual, and 12 local immunotherapy) and 14 were controls. the results were compared with the clinical course to determine possible associations between serum and clinical changes. subcutaneous, but not sublingual and nasal, immunothe ... | 1993 | 7755664 |
| antigen avoidance in a mountain environment: influence on basophil releasability in children with allergic asthma. | the influence of natural antigen avoidance in an environment free of relevant allergens (istituto pio xii, misurina, italian alps, 1756 m) and of antigen exposure (sea level) on basophil releasability, as well as on bronchial hyperreactivity (bhr) and specific ige serum level, were investigated in a group of children with asthma who were allergic to dermatophagoides pteronyssinus. | 1993 | 7693784 |
| optimization of skin testing. i. choosing allergen concentrations and cutoff values by factorial design. | standardized extracts of phleum pratensis (grass) and dermatophagoides pteronyssinus (house-dust mite) were used as test allergens for multiple regression in order to determine optimum concentrations and cutoff values with regard to diagnostic capacity and safety. if a rast value of class 1 or more was taken as an indication of sensitization, it was found that the optimum concentrations for the detection of sensitization are in the range 10-100 bu/ml and 1500-10,000 bu/ml for intracutaneous test ... | 1993 | 7694518 |
| no increase in plasma histamine during paf-induced airway obstruction in allergic asthmatics. | to investigate the possible role of mast cell or basophil histamine release in mediating platelet-activating factor (paf) airway obstruction, we studied the effect of inhaled paf (30 micrograms, single dose) on plasma histamine, bronchial caliber, and leukocyte and platelet counts in six patients with mild or moderate allergic asthma (mean age, 27 +/- 1.3 years; mean fev1, 95 +/- 5 percent of predicted; mean pc20 methacholine, 1.46 +/- 0.36 mg/ml). specific conductance (sgaw) fev1, fef25-75 perc ... | 1993 | 8365293 |
| intra-species cross-reactivity of house dust mite allergens separated by protein blotting and detected by selective elution of mite components and ige antibodies. | a complex and varied antibody response to allergens, in particular dermatophagoides pteronyssinus, is observed in the serum of allergic patients. to examine the role of cross-reacting antigenic determinants in allergy, a strategy has been devised for isolating antibody populations that bind to individual polypeptides of an allergen source separated by sodium dodecyl sulfate-polyacrylamide electrophoresis and electroblotted to nitrocellulose. polypeptides are identified on the blots, desorbed and ... | 1993 | 8223402 |
| release of chemical mediators and inflammatory cell influx during early allergic reaction in the nose: effect of furosemide. | we evaluated the effect of furosemide on allergen-induced rhinitis in a double-blind, crossover, placebo-controlled experiment. | 1993 | 8349935 |
| a prospective safety-monitoring study of immunotherapy with biologically standardized extracts. | we evaluated the safety of immunotherapy in 419 patients who attended our allergy department for treatment. they were suffering from rhinitis and asthma caused by sensitization to grass pollen or dermatophagoides pteronyssinus. immunotherapy was given by biologically standardized aluminum hydroxide adsorbed extracts according to a conventional schedule. local reactions were recorded in 10.5% of the patients and systemic reactions in 4.8%. only 0.37% of the doses administered were associated with ... | 1993 | 8238801 |
| neutrophil chemotactic activity (nca) in nasal secretions from atopic and nonatopic subjects. effect of antigen challenge. | in order to elucidate the mechanism responsible for infiltration of nasal mucosa by granulocytes, we tested neutrophil chemotactic activity (nca) in nasal lavages, by the modified boyden chamber method, in 16 patients with perennial allergic rhinitis (ar), six asa-sensitive patients with chronic rhinosinusitis (crs), and seven normal, nonatopic control subjects (nc). nasal secretions from all three groups showed significant nca (mean 157.1 +/- 54.0, 62.2 +/- 20.7, and 39.4 +/- 11.4% of fmlp chem ... | 1993 | 8238796 |
| lymphocyte transformation test with house dust mite (dermatophagoides pteronyssinus) in normal children, asthmatic children and asthmatic children receiving hyposensitization. | in the first part of this study the proliferative response of lymphocytes (lymphocyte transformation test) to house dust mite (hdm) stimulation in cultures was studied in normal children (n = 16), asthmatic children who never received hyposensitization (hs) (n = 50) and asthmatic children receiving hs with hdm for at least 6 months (n = 20). the results are expressed as disintegrations per minute (d.p.m.) and as stimulation index (si = d.p.m. in the presence of the allergen/d.p.m. in the control ... | 1993 | 8221269 |
| does guanine concentration in house-dust samples reflect house-dust mite exposure? | using specific elisa extracts, we analyzed samples of pure mite cultures and house dust for group i allergens from dermatophagoides pteronyssinus, d. farinae, and d. microceras. correspondingly, we measured the concentration of guanine and xanthine by rigorous chemical methods. the pure mite cultures were used to correlate allergen levels with guanine levels. the house-dust samples one-sidedly contained more guanine than expected. this suggests that considerable amounts of guanine originate from ... | 1993 | 8368454 |
| mite allergen content in mattress dust of dermatophagoides-allergic asthmatics/rhinitics and matched controls. | it has been suggested that the mites dermatophagoides pteronyssinus and d. farinae are important indoor environmental factors facilitating both the sensitization of atopic subjects and asthmatic attacks of house dust-sensitive patients. contradictory results have been reported about the current exposure to mites or their allergens among patients and control groups of atopic or non-atopic subjects. in order to determine whether there is a difference in mite exposure levels between d. pteronyssinu ... | 1993 | 8221261 |
| significant reduction of nonspecific bronchial reactivity in patients with dermatophagoides pteronyssinus-sensitive allergic asthma under therapy with allergen-antibody complexes. | thirty-nine asthmatic patients hypersensitive to dermatophagoides pteronyssinus were treated for a total of 4 yr with injections of complexes made of allergen and autologous specific antibodies. the results obtained throughout the first 2 yr of a double-blind placebo-controlled trial have been published (1) and we now report the results of such therapy during an additional 2 yr. three groups of patients had been defined: groups a and b were comprised of patients treated with either "higher" dose ... | 1993 | 8503552 |
| effect of oral and inhaled cetirizine in allergen induced bronchoconstriction. | cetirizine is a potent, selective h1 histamine receptor antagonist. the effect of oral and inhaled cetirizine was assessed on the early bronchoconstrictor response to inhaled allergen in 10 mild atopic asthmatic patients in a double-blind, randomized, placebo controlled trial. all were sensitive to dermatophagoides pteronyssinus and this was used as the provoking allergen. the geometric mean pd20 fev1 values obtained at allergen challenge were measured as cumulative breath units (c.b.u.) and fol ... | 1993 | 8369980 |
| reproducibility of allergen-induced asthma and associated increase in bronchial responsiveness to methacholine in asthmatic children. | we studied the reproducibility of early (ear) and late (lar) asthmatic response to allergen challenge in 13 asthmatic children (four girls, age range: 10 to 17 years) sensitized only to dermatophagoides pteronyssinus (dp). further, changes in bronchial responsiveness to inhaled methacholine following lar were examined by measuring pc20fev1 methacholine after 24, 48, and 72 hours. we carried out two carefully controlled allergen challenges with the same allergen dose within 4 to 6 weeks, at least ... | 1993 | 8498734 |
| allergens from dermatophagoides mites with chymotryptic activity. | a new set of allergens from dermatophagoides pteronyssinus and d. farinae (provisionally named dp5 and df5, respectively) was isolated from the whole culture of mites. the apparent molecular weights of both allergens were shown to be 25,000 on sds-page under a reducing condition and 27,000 on sephadex g-75 gel filtration chromatography. both dp5 and df5, as well as der f iii, possessed proteolytic activity. the results of substrate specificity and susceptibility to various protease inhibitors of ... | 1993 | 8334537 |
| occurrence of ige antibodies against the 39-kda allergen component of the mite lepidoglyphus destructor in urban and rural subjects. | several species of storage mite have been shown to cause ige-mediated sensitization. monoclonal antibodies (mabs) have been raised against the storage mite lepidoglyphus destructor, and one mab (42b6) immunoprecipitated a band of 39 kda of l. destructor. to search for the frequency of ige antibodies in patient sera against this allergen component, we modified a radioimmunoassay (ria), 42b6 being used as capturing antibody. twenty of 43 sera from farmers who were rast positive to l. destructor (4 ... | 1993 | 8506991 |
| mite asthma in childhood: a study of the relationship between exposure to house dust mites and disease activity. | children with asthma are commonly sensitized to the house dust mite. | 1993 | 8473672 |
| intraepithelial dendritic cells and selective activation of th2-like lymphocytes in patients with atopic asthma. | this study examines the distribution of intraepithelial dendritic cells in eight atopic patients with symptomatic asthma and their ability to induce activation of autologous t lymphocytes in vitro. all subjects were sensitized to dermatophagoides pteronyssinus. the incubation of asthmatic epithelial cells and dendritic cells with autologous resting cd4-positive t cells and purified extracts of d pteronyssinus induced t cell activation and release of high levels of interleukin-4 (il-4) and interl ... | 1993 | 8131514 |
| natural killer cell stimulatory factor (interleukin 12 [il-12]) induces t helper type 1 (th1)-specific immune responses and inhibits the development of il-4-producing th cells. | the effects exerted on the in vitro development of antigen-specific t cell lines and t cell clones by addition or neutralization of interleukin 12 (il-12) in lymphocyte bulk culture were examined. t cell lines specific for dermatophagoides pteronyssinus group i (der p i) derived in the presence of il-12 exhibited reduced ability to produce il-4 and increased ability to produce interferon gamma (ifn-gamma), and developed into der p i-specific cd4+ t cell clones showing a t helper type 0 (th0)- or ... | 1993 | 8096238 |
| a double-blind, placebo controlled trial of solidified benzyl benzoate applied in dwellings of asthmatic patients sensitive to mites: clinical efficacy and effect on mite allergens. | the aim of this double-blind, randomized study was to investigate the effectiveness of an acaricidal cleaning product in modifying both clinical symptoms and mite allergen levels over a period of at least 1 year. | 1993 | 8454796 |
| a controlled dose-response study of immunotherapy with standardized, partially purified extract of house dust mite: clinical efficacy and side effects. | seventy-four asthmatic patients allergic to house dust mite were included in a double-blind, controlled study to establish the optimal maintenance dose of a standardized extract of dermatophagoides pteronyssinus (der p i) during 24 months of immunotherapy (it). | 1993 | 8454793 |
| duration of preventive effect of inhaled salbutamol on early airway response to allergen in asthmatic subjects. | salbutamol is known to effectively prevent early asthmatic response (ear) after specific bronchial provocation test (sbpt) in asthmatic subjects, but the time-course of this protection is not known. in this study the effects of 200 micrograms salbutamol inhaled 2 h before sbpt were compared with 200 micrograms salbutamol inhaled 10 min before sbpt in eight asthmatic subjects sensitized to phleum pratensis (pp) or dermatophagoides pteronyssinus (dp). all subjects showed an ear (% decrease in fev1 ... | 1993 | 8497681 |
| secretion of il-2, il-4, and ifn-gamma for dust mite antigens in a patient with atopic dermatitis. | in a 21-year-old female with severe atopic dermatitis, the secretion of interleukin-2 (il-2), interleukin-4 (il-4), and interferon-gamma (ifn-gamma) by her peripheral blood mononuclear cells (pbmc) was measured after incubation with/without antigens extracted from dermatophagoides pteronyssinus (dp), to which the patient had developed a positive patch-test reaction. incubation with dp antigen produced marked secretion of il-2 and ifn-gamma, but not il-4. this may suggest that dp-specific t lymph ... | 1993 | 8478491 |
| house-dust mites and associated environmental conditions in danish homes. | the concentration of house-dust mites (dermatophagoides pteronyssinus) was investigated in 96 danish homes with one or more members suffering from asthma. the air-exchange rates, humidity, and temperature were measured in all the homes. a positive correlation was found between indoor air humidity and mite concentration (p < 0.01), and an inverse correlation (p = 0.027) between house-dust mite concentration and indoor air-exchange was found. homes of mite-allergic persons had a higher concentrati ... | 1993 | 8457030 |
| peripheral blood mononuclear cells from house dust mite allergic patients produce il-2 in response to specific allergen challenge. | proliferative responses and production of il-2 by peripheral blood mononuclear cells of patients with dermatophagoides pteronyssinus rhinitis +/- asthma were measured at up to 24 hr intervals from 1-8 days, after stimulation with mitogen (pha), antigen (sksd) and specific allergen (d. pteronyssinus). il-2 was measured in culture supernatants using the murine ctll cell line bioassay. compared to non-atopic controls, patients had significantly higher proliferative responses to d. pteronyssinus and ... | 1993 | 8448681 |
| a novel therapy for atopic dermatitis with allergen-antibody complexes: a double-blind, placebo-controlled study. | exposure to airborne allergens exacerbates symptoms of atopic dermatitis (ad) in hypersensitive patients. | 1993 | 8432921 |
| comparison of antigen presentation by lymph node cells from protein and peptide-primed mice. | lymph node cells from mice primed with peptides from the allergens der p i and der p ii (the group i and ii allergens of dermatophagoides pteronyssinus) were unable to recall responses to the protein antigen when cultured in vitro despite being able to mount large responses to the peptides. the t cells could however recall responses to the protein when spleen-adherent cells were added into culture. treating the spleen accessory cells with the monoclonal antibody (mab) 33d1 and complement largely ... | 1993 | 8436404 |
| specific ige antibodies in nasal secretions: correlation with serum values and clinical tests. | the presence of specific ige in external secretions and the possibility of quantifying it has aroused the interest of many research workers. nevertheless, there is still disagreement with regard to how useful this practice is from a clinical point of view. we have compared the presence of dermatophagoides pteronyssinus (dp) specific-ige in the nasal secretion of 17 patients suffering from rhinitis with the results of prick tests and nasal and/or conjunctival challenge tests. as a preliminary ste ... | 1993 | 8424593 |
| decrease of skin and bronchial sensitization following short-intensive scheduled immunotherapy in mite-allergic asthma. | changes induced by high-dose intensive specific immunotherapy (isi) were studied in 18 patients with mite-allergic bronchial asthma and compared with 18 control patients. a biologically standardized well-characterized dermatophagoides pteronyssinus extract of known potency was used both for in vivo tests and isi. immediate d pteronyssinus skin tests (measured by parallel-line bioassay) were reduced 20-fold in isi treated patients, and delayed skin responses significantly decreased and even disap ... | 1993 | 8417875 |
| protective effect of nedocromil sodium on the dual asthmatic response to bronchial antigen challenge. | the effects of nedocromil sodium and placebo on the asthmatic response to antigen bronchial provocation were studied in a double-blind, randomized, crossover trial. twelve subjects sensitized to dermatophagoides pteronyssinus were challenged with successive doubling doses of this allergen (1.25-80 x 10(-3) mg) on two separate occasions, at least 15 days apart. a single dose of either nedocromil sodium 4 mg, or, a matching placebo was given 30 min before challenge. forced expiratory volume in 1 s ... | 1993 | 8385793 |
| sensitization of asthmatic children to common environmental allergens according to their residence. | the medical records of 974 asthmatic children aged 1-14 years (mean 8.7 +/- 3.9 years) who had been evaluated with skin prick tests (spt) in two referral children's hospitals in athens from 1975 to 1987 were analyzed. the children were grouped according to their residence into groups from urban area (ur), rural area (ru), and coastals (co). the prevalence of positive spt and the sensitizing allergens according to the residential area and the family atopic history were considered. it was found th ... | 1993 | 8244914 |
| expression of lymphocytes fc epsilon rii/cd23 in allergic children undergoing hyposensitization. | owing to the proposed role of fc epsilon rii/cd23 in allergic diseases, we analyzed the expression of this receptor on peripheral blood lymphocytes (pan-b, pan-t and cd4+ or cd8+ t cells) and its autoproteolytic product scd23 in serum. this was done in 10 asthmatic children allergic to dermatophagoides pteronyssinus (dpt) before and 6 weeks after hyposensitization. facs analysis of double, direct immunofluorescence staining of the whole blood revealed an elevated percentage of fc epsilon rii/cd2 ... | 1993 | 7685220 |