Publications

immune responses of swine to oral inoculation with embryonated eggs of ascaris suum.	responses of swine to oral inoculation with embryonated eggs of ascaris suum were monitored, using lymphocyte blastogenesis assays, indirect radioimmunoassays, and peripheral eosinophil counts (ec). transient cell-mediated immune responses of peripheral lymphocytes were detected by lymphocyte blastogenesis assay as early as postinoculation day (pid) 2, but were rarely positive for consecutive samples taken at 2-day intervals. humoral antibodies were first detected at pid 6 to 17 by indirect radi ...	1982	6216835
phosphofructokinase from ascaris suum muscle.		1982	6218373
antigenicity of beta-lactam antibiotic preparations: production of ige antibodies to beta-lactam antibiotic and their cross-reaction within the antibiotic group.	balb/c mice were immunized with conjugate of benzylpenicillin or ampicillin with ascaris suum extract. the mice developed ige antibodies to penicillin, which were found to react with commercially available penicillin preparations in the pca system. elimination of polymerized penicillin by sephadex chromatography from tested preparations could not diminish their activities to elicit pca. high pressure liquid chromatographic (hplc) separation of pcg preparation yielded fractions unable to elicit p ...	1982	6176550
phosphofructokinase from ascaris suum. purification and properties.	a rapid and efficient procedure has been developed to purify phosphofructokinase from the muscle of the parasitic roundworm, ascaris suum. the procedure can be accomplished in 1 day with a 420-fold purification and a 60% yield. the enzyme was shown to be homogeneous by two-dimensional electrophoresis, sepharose 6b column chromatography, and high performance liquid chromatography utilizing a size exclusion column. the subunit molecular weight of the enzyme was found to be 95,000 by electrophoresi ...	1982	6460770
phosphofructokinase from ascaris suum. regulatory kinetic studies and activity near physiological conditions.		1982	6460771
phosphofructokinase from ascaris suum. the effect of phosphorylation on activity near-physiological conditions.		1982	6460772
[eosinophilia in white mice in experimental toxocariasis and recurrent ascariasis].	twenty-five white mice were experimentally invaded by the larvae of toxocara cati, and their blood count was followed. on the seventh day after invasion the number of eosinophil leucocytes started to increase and the maximum (28%) was reached on the twenty-first day. after 45 days the mice were newly invaded by eggs of ascaris suum. the number of eosinophil leucocytes increased again and reached its maximum on the fourteenth day (26%). the increased eosinophilia in mice was observed until the 20 ...	1982	6812263
[changes in the number of t and b lymphocytes in the blood of pigs after experimental ascaridiosis].	the changes in the number of the t and b dependent cells in the peripheral blood were studied after experimental invasion of piglets (live weight 8-10 kg) with the eggs of ascaris suum (50 000 eggs per piglet). the detection of both cell populations by two rosette methods (e and eac) showed that their first rise set in on the third day from invasion. the maximum occurrence of the t and b cells was recorded 17 days from invasion; on the 20th day from invasion their number returned to the initial ...	1982	6818751
effects of nitric acid on carbachol reactivity of the airways in normal and allergic sheep.	the airway effect of a 4-hr exposure (via a plexiglas hood) to 1.6 ppm nitric acid vapor were evaluated in seven normal and seven allergic sheep, i.e., animals that have a history of reacting with bronchospasm to inhalation challenge with ascaris suum antigen. the nitric acid vapor was generated by ultrasonic nebulization of a 2% nitric acid solution. airway effects were assessed by measuring the change in specific pulmonary flow resistance before and after a standard inhalation challenge with 2 ...	1982	6277256
correlation of muscle activity with glycogen metabolism in muscle of ascaris suum.	isolated muscle segments from the parasitic roundworm ascaris suum were shown to contract when perfused with acetylcholine (ach). the muscle responded to ach concentrations of 1 microm and was maximally contracted at 50 microm ach. in fed muscle segments perfused with saturating levels of ach the glycogen synthase ka values for glucose 6-phosphate increased from 0.5 to 0.95 mm. in starved segments stimulated by ach, the muscle utilized glycogen at a rate that was 1.41 micrograms.min-1.g tissue-1 ...	1982	6282145
chromatin diminution in ascaris suum: nucleotide sequence of the eliminated satellite dna.	we have determined the prototype sequence of the dna which is eliminated in the course of chromatin diminution in ascaris suum. this dna which is virtually absent from somatic cells but retained in the germ line consists predominantly of highly repetitive sequences which are variants of an at rich 123 base pair repeat unit. both major and minor variants have been sequenced. the overall structure of this germ line limited dna corresponds to the segmental organization characteristic of satellite d ...	1982	6285304
enzyme polymorphism in ascaris suum (nematoda).	the potential of multilocus electrophoretic studies for providing insight into the population biology of parasitic organisms was studied using the swine parasite ascaris suum suum. thirty-eight loci encoding enzymatic or nonenzymatic proteins have been resolved in extracts of adult worms by starch-gel electrophoresis. a preliminary study of variation in ascaris from eastern iowa revealed an average heterozygosity of 6.6%. allele frequencies at six polymorphic loci were similar in males and femal ...	1982	6889643
development of immune responsiveness to ascaris suum antigens in pigs vaccinated with ultraviolet-attenuated eggs.	pigs fed ascaris suum eggs attenuated by short wave ultraviolet-radiation developed resistance to challenge infection. per os inoculation of pigs on three successive weeks with 10,000 eggs irradiated to total exposures of 150,100 and 75 microw-min/cm2, respectively, resulted in an 88% reduction in the number of larvae recovered from the lungs 7 days after challenge with 10,000 infective eggs. peripheral blood lymphocytes (pbl) from vaccinated pigs were specifically stimulated in vitro to incorpo ...	1982	6889778
ascaris suum: oxidative phosphorylation in mitochondria from developing eggs and adult muscle.		1982	6889977
determination of the specific gravity of certain helminth eggs using sucrose density gradient centrifugation.	the specific gravities of ten species of helminth eggs were determined using sucrose density gradient centrifugation. fecal or egg concentrate was layered over a 3 to 54% sucrose density gradient. the gradient was then centrifuged at 800 g for 20 min, allowing 5 min for acceleration and 5 for deceleration. bands formed were identified and measured. refractive index was measured at the middle of narrow bands, or at the level at which the concentration of eggs was highest, in the case of wide band ...	1982	6890102
reversible inhibition of hatching of infective eggs of ascaris suum (nematoda).		1982	6890547
experimentally induced resistance to ascaris suum in pigs.	the present study shows that oral inoculation with infective a. suum eggs or 3rd stage larvae in pigs aged 5-16 weeks induces resistance against oral challenge with infective a. suum eggs. acquired resistance is reflected by an adaptive type of host response as measured by increased pathological, serological and hematological responses and decreased recovery of worms from lungs and small intestine. although based on a limited number of experimental pigs, the results of the present experiments su ...	1982	6890668
survival rates of parasite eggs in sludge during aerobic and anaerobic digestion.	the effects of mesothermic anaerobic or aerobic sludge digestion on survival of eggs from the roundworms ascaris suum, toxocara canis, trichuris vulpis, and trichuris suis and from the rat tapeworm hymenolepis diminuta were studied. destruction of eggs throughout a 15-day treatment period, as well as their viabilities after reisolation, was analyzed. the laboratory model digesters used in this study were maintained at a 15-day retention schedule, partially simulating a continuously operating sys ...	1982	6891199
evaluation of three immunodiagnostic procedures for porcine strongyloidiasis.	an indirect fluorescent antibody test (ifat), an indirect radioimmune antibody test (irat), and an enzyme-linked immunosorbent antibody assay (elisa) were compared for their efficacies in detecting antibody to strongyloides ransomi in pigs. the greatest sensitivity was exhibited by the elisa, using a soluble, whole larval extract. the 2 indirect antibody assays, ifat and irat, using intact 3rd-stage larvae (l3) were comparable in sensitivities. the use of live l3 in the ifat and irat presented t ...	1982	7041716
genetic control of eosinophilia in mice: gene(s) expressed in bone marrow-derived cells control high responsiveness.	a heterogeneity in the capacity of strains of mice to mount eosinophilia is described. balb/c and c3h are eosinophil high responder strains (eo-hr) and cba and a/j are eosinophil low responder strains (eo-lr), judged by the response of blood eosinophils to ascaris suum, and the response of blood, bone marrow, and spleen eosinophils to keyhole limpet hemocyanin given 2 days after 150 mg/kg cyclophosphamide. some of the gene(s) for high responsiveness appear to be dominant because (eo-hr x eo-lr)f ...	1982	7054293
purification and properties of phosphoenolpyruvate carboxykinase from ascaris suum.		1982	7076387
[the larva precipitation test with ascaris suum and toxocara canis larvae].	investigations were made by means of a larvae precipitation test (lpt) on different laboratory animals (pigs and white mice, and pigs, rabbits, guinea pigs and white mice respectively) experimentally infected with ascaris suum and toxocara canis respectively for the serological evidence of specific a. suum and t. canis antibodies. living a. suum larvae isolated from the lungs of experimentally infected white mice forming oral, excretion porus and cuticula precipitates proved to be a suitable ant ...	1982	7081762
role of ciliary motility in acute allergic mucociliary dysfunction.	the purpose of this investigation was to determine if abnormal ciliary function contributes to allergic mucociliary dysfunction. in conscious sheep with ascaris suum hypersensitivity, ciliated cells were obtained with a cytology brush and tracheal mucous velocity (tmv) was determined before and serially for 2 h following antigen inhalation. the recovered cells (also containing mast cells) were suspended in a chamber, and ciliary activity was viewed microscopically and recorded on videotape for s ...	1982	7085401
[protective properties of the cavitary fluid antigens of ascaris suum].	fractions of cavitary fluid of ascaris (cfa) containing antigens were obtained on membranous filters. being administered to mice they provide the development of protective immunity of animals to their subsequent infection with invasional eggs of a. suum. in mice immunized with fraction cfa xm-50 the number of a. suum larvae obtained from the lungs on the 7th day of invasion was confidentially lower than in control animals (15.277) +/- 1.825 on the 7th day of invasion was confidentially lower tha ...	1982	7122119
mitochondrial nadh oxidase activity of adult hymenolepis diminuta (cestoda).	1. mitochondria from adult hymenolepis diminuta displayed a membrane-associated, rotenone-sensitive nadh oxidase and nadh-dependent fumarate reductase system. 2. both the h. diminuta oxidase and fumarate reductase were relatively insensitive to antimycin a. potassium cyanide and sodium azide, at concentrations which significantly inhibited the nadh oxidase system of rat liver. 3. malonate effectively depressed the mitochondrial nadh oxidase activity of both h. diminuta and adult ascaris suum (ne ...	1982	7128112
ascaris suum: differential effects of avermectin b1a on the intact animal and neuromuscular strip preparations.		1982	7128715
studies on chemotherapy of parasitic helminths (vii). effects of various cholinergic agents on the motility of angiostrongylus cantonensis.	effects of various cholinergic agents on the motility of angiostrongylus cantonensis were studied to define the neuropharmacological properties of this worm. stimulation of the motility and/or contraction were shown by eserine, ach, carbachol, nicotine, dmpp, pyrantel, and ba2+, but not by pilocarpine and mcn-a-343. contraction was similarly observed by these agents in the preparations paralyzed with praziquantel. paralysis was caused remarkably by d-tubocurarine and slightly by succinylcholine, ...	1982	7131950
a radioallergosorbent test (rast) for ascaris lumbricoides.	an antigen from ascaris lumbricoides obtained from infected humans has been prepared which can be used for detecting immune response to ascaris using the skin-prick test. a comparison of immune skin responses between this antigen and the commercial (bencard) ascaris suum antigen has been made and a rast technique for the in vitro detection of specific ige to ascaris lumbricoides has been developed using the lumbricoides antigen. a correlation between skin-prick test and rast determinations and b ...	1982	7137527
electrophysiological effects of piperazine and diethylcarbamazine on ascaris suum somatic muscle.	1 electrophysiological recordings were made from the bag region of ascaris suum muscle. membrane potential and input conductance or membrane current under voltage clamp were measured. 2 in high-cl- ringer, bath-applied piperazine, at concentrations greater than 10(-4)m, produced a dose-dependent and reversible increase in input conductance associated with a hyperpolarizing potential. the increase in input conductance was reduced when the preparations were bathed in low-cl- ringer. gamma-aminobut ...	1982	7139188
cross resistance between ascaris suum and nippostrongylus brasiliensis in mice.	the present study shows that n. brasiliensis infection in thymus-bearing mice results in resistance against a subsequent a. suum infection. in contrast thymus-deficient mice do not develop this heterologous resistance. thymus-deficient mice, on the other hand, develop resistance to homologous challenge although to a lesser degree than thymus-bearing mice. further, it is shown that a. suum infection in thymus-bearing mice yields resistance against a subsequent n. brasiliensis infection. the under ...	1982	7145653
hexokinase from ascaris suum muscle.		1982	7154947
fructose-bisphosphate aldolase from ascaris suum.		1982	7154952
migration of some roundworm species in experimentally infected white mice.	the migration of four roundworm species (ascaris suum, toxocara canis, t. cati and toxascaris leonina) was studied in various organs of experimentally infected white mice. the hatching of t. leonina and t. cati larvae in the paratenic host is more rapid (24-36 h) than the hatching of a. suum and t. canis larvae (1-6 days). the deposition of larvae in host organs is different in the individual species. maximum number of larvae in the liver: t. leonina and a. suum on day 4, t. canis on day 2 and t ...	1982	7160786
inhibition of human blood clotting by extracts of ascaris suum.	the effects of soluble extracts of ascaris suum on human blood coagulation were investigated. whole worm supernatant solution prolonged the whole blood clotting time and the kaolin-activated, partial thromboplastin time but it did not alter the prothrombin time. these data indicate impairment of the intrinsic pathway of blood coagulation. whole worm supernate inhibited platelet aggregation induced by adp and ristocetin. this supernate did not affect fibrinolysis. the maximal concentration of ant ...	1982	7175610
purification and characterization of excretory and secretory antigen of toxocara canis larvae.	excretory/secretory (es) antigen of toxocara canis larvae was isolated from the culture medium of second-stage larvae by gel filtration. the antigen induced production of not only igm or igg but also ige antibody. the antigen, when treated with guanidine hydrochloride and 2-mercaptoethanol, lost its allergenic activity but retained an ability to induce igm or igg antibody. purified antigen was of glycoprotein nature and had a molecular weight of 35,000. the whole antigen showed a cross-reaction ...	1983	6411609
pulmonary uptake of mannitol as an index of changes in lung epithelial permeability.	to develop a simple, inexpensive, and nontoxic test for changes in bronchial permeability we have employed a specific gas chromatographic assay to measure the uptake of mannitol from the lung after administration of an intratracheal aqueous bolus (8 ml of 5% solution). we have tested the ability of our method to detect the known increase in lung epithelial permeability elicited by challenge of previously sensitized animals with aerosols of ascaris suum antigen. in ascaris-sensitive basenji-greyh ...	1983	6413466
purification and characterization of phosphorylase b from ascaris suum.	glycogen phosphorylase b (ec 2.4.1.1) has been purified from the muscle of the roundworm, ascaris suum. the 223-fold purified enzyme was shown to be homogenous by high performance liquid chromatography (hplc), gel filtration column chromatography and sodium dodecyl sulfate (sds) gel electrophoresis. the apparent native molecular weight of the enzyme determined by size exclusion chromatography by hplc and gel filtration corresponded to 200 000 and 199 000, respectively. the subunit molecular weig ...	1983	6419099
the role of cyclic amp-mediated regulation of glycogen metabolism in levamisole-perfused ascaris suum muscle.	the effects of levamisole on muscle contraction and glycogen metabolism have been examined in isolated muscle-cuticle sections of the roundworm ascaris suum. muscle contraction occurred when various levels of levamisole were perfused through the preparation. at a levamisole concentration of 0.42 mm, the period of contraction lasted only about 6 min and was followed by a period of relaxation. during this relaxation period, there was an activation of glycogen synthase (ec 2.4.1.11), as evidenced b ...	1983	6300647
functional depression of h2 histamine receptors in sheep with experimental allergic asthma.	we tested the hypothesis that airway hyperresponsiveness to histamine in the allergic sheep is related to functional depression of h2 histamine receptors. thirteen of 32 sheep responded with bronchoconstriction to inhaled ascaris suum antigen (allergic sheep), and the remainder served as controls (nonallergic sheep). in the allergic sheep, 50 and 100 breaths of 5% histamine solution increased mean pulmonary resistance (rl) to 235% and 438% of baselines, respectively. the corresponding values in ...	1983	6136540
effects of 8-(2-fluoroethyl)-3 alpha-hydroxy-1 alpha h, 5 alpha h-tropaniumbromide benzilate (ba598br) on allergy- and drug-induced asthmas.	the effects of ba598br, a new atropine derivative possessing a quarternary ammonium salt structure, on the canine airway, including its antiasthmatic effects, were investigated. the in vivo airway resistance was determined using the modified konzett-rössler method. inhalation of 0.01% of ba598br had an inhibitory effect against acetyl-choline (ach, 10 micrograms/kg i.v.)-evoked bronchoconstriction. the effect of ba598br was more powerful and longer lasting than that of the same dose of atropine. ...	1983	6139497
detection and characterization of polymers in cephalothin by passive cutaneous anaphylaxis in mice.	immunization of balb/c mice by repeated injections of cephalothin (cet) - ascaris suum extract conjugate resulted in formation of ige antibodies, which were able to sensitize syngeneic animals for passive cutaneous anaphylaxis (pca). by means of high-pressure liquid chromatography, a fraction with extremely high pca-eliciting activity, but without appreciable antimicrobial activity, was isolated from the cet preparation. physicochemical analyses of the fraction identified the major component of ...	1983	6822439
ultrastructure and function of oviduct-uterine junction in ascaris suum (nematoda).	cells comprising the oviduct-uterine junction of the female reproductive tract in ascaris suum were shown to have a distinctive ultrastructure. the free luminal surface of each epithelial cell was bordered by an electron-dense layer that measured 90 to 120 nm in thickness and could be removed by treatment with pronase. numerous membrane-bound, filament-containing bodies were present in the cytoplasm near the apical margin. these filaments, measuring 6.5 nm in diameter, coalesce (or condense) to ...	1983	6827433
modification of allergic bronchoconstriction by a calcium antagonist: mode of action.	because both degranulation of mast cells and contraction of airway smooth muscle are dependent upon the influx of calcium, a calcium blocking agent might modify allergic bronchoconstriction by at least these two mechanisms. we treated sheep allergic to ascaris suum antigen with the calcium antagonist verapamil prior to airway challenge with an aerosol of ascaris suum antigen and also investigated the response without pretreatment. aerosolized ascaris suum antigen increased mean pulmonary resista ...	1983	6859650
changes in the blood picture of white mice experimentally infected with various species of ascarids.	the blood picture was studied in white mice experimentally infected with ascaris suum, toxocara cati, and toxascaris leonina. in mice infected with a. suum, maximum number (17%) of eosinophiles occurred on day 28 p.i. in mice infected with t. cati, the eosinophilia increased already from the first day after infection, reaching the maximum (26%) on day 21 p.i. in mice infected with t. leonina, the eosinophilia increased from day 7 to day 28 p.i. and a slightly increased number of eosinophiles per ...	1983	6862317
ribonucleotide levels in six nematodes, a cestode and a trematode.	the levels of ribonucleotides in the nematodes haemonchus contortus, ostertagia circumcincta, trichostrongylus colubriformis, nippostrongylus brasiliensis, ascaris suum and stephanurus dentatus, the cestode moniezia expansa and the trematode fasciola hepatica were measured by high pressure liquid chromatography. the adenylate charge, atp and total adenine nucleotide levels were in general agreement with those determined enzymatically and with published data. udp and guanine nucleotide levels wer ...	1983	6877282
regulation of the ascaris suum pyruvate dehydrogenase complex by phosphorylation and dephosphorylation.	the pyruvate dehydrogenase complex isolated from 'anaerobic' mitochondria of ascaris suum has a subunit composition similar to complexes isolated from most other eukaryotic organisms and is regulated by phosphorylation and dephosphorylation. pyruvate dehydrogenase kinase activity is stimulated by nadh and a number of physiologically important acyl-coa intermediates and is inhibited by coa, propionate, tiglate and pyruvate. it is suggested that the elevated levels of pyruvate observed in the asca ...	1983	6877284
flubendazole: dose range and efficacy studies against common internal parasites of swine.	the anthelmintic spectrum of activity and efficacy of flubendazole, using pigs naturally infected with helminth parasites, were determined in 4 experiments, using 157 pigs. flubendazole (1.5 mg/kg of body weight) mixed in feed or top-dressed on feed for 5 consecutive days was highly effective as an anthelmintic. this dosage level indicated 100% efficacy against mature ascaris suum, oesophagostomum dentatum, trichuris suis, and metastrongylus apri. there was 88% efficacy against strongyloides ran ...	1983	6881670
in vitro interaction of eosinophils from ascarid-infected eyes with ascaris suum and toxocara canis larvae.	the role of eosinophils in ocular ascarid infection is studied in an animal model. primary intravitreal infection of guinea-pig eyes with ascarid (toxocara canis, ascaris suum) second-stage (l2) larvae resulted in an anterior chamber exudate containing 98% or more eosinophils. anterior chamber aspirates were cultured in rpmi media 1640 with t. canis or a. suum l2 larvae at 37 degrees c for 1-96 hours. the in vitro interaction of eosinophils with l2 larvae was studied by light, and scanning and t ...	1983	6618795
duration of mucociliary dysfunction following antigen challenge.	antigen-induced bronchospasm is associated with an impairment of airway mucociliary clearance, but the duration of this defect has not been examined. in the present study, we determined the time course of mucociliary dysfunction following inhalation challenge with ascaris suum extract in conscious sheep with hypersensitivity to this antigen. tracheal mucous velocity (tmv) as an index of mucociliary function, pulmonary resistance (rl), and arterial oxygen tension (pao2) were measured prior to, im ...	1983	6629909
factors contributing to the in vitro development of ascaris suum from second-stage larvae to mature adults.	when a three-step roller culture system was used, second-stage larvae of ascaris suum, artificially hatched from eggs, developed in high numbers to the fourth stage, and a few to young and mature adults. the culture system consisted of (1) medium kw-2 supplemented with 10 mm l-cysteine for the first 4 days, and with 5 mm l-cysteine for the following 7 days; (2) followed by medium api-18 for 7 days; and (3) thereafter, by medium api-1 supplemented with hemin (bovine) at a concentration of 24 micr ...	1983	6631626
characterization of a late phase pulmonary response after antigen challenge in allergic sheep.	we determined if allergic sheep undergo a dual bronchoconstriction in response to inhalation of antigen and whether these responses could be modified by cromolyn or glucocorticosteroids. in 8 conscious sheep with ascaris suum hypersensitivity, mean pulmonary flow resistance (rl), dynamic lung compliance (cdyn), thoracic gas volume (vtg), and arterial oxygen tension (pao2) were determined prior to, immediately after, and from 2 through 8 h after and 24 h after inhalation challenge with ascaris su ...	1983	6638671
antigen stimulates glycoprotein secretion and alters ion fluxes in sheep trachea.	we studied the effects of in vitro challenge with specific antigen (ascaris suum antigen) on glycoprotein secretion and ion fluxes in tracheal tissues from allergic sheep. we mounted tissues in perspex chambers and measured secretion of 35s- and 3h-labeled glycoproteins and fluxes of cl- and na+. in tissues from allergic sheep, a. suum antigen (25 micrograms protein x ml-1) increased glycoprotein secretion. a. suum antigen initially reversed net cl- flux, causing net absorption of cl- and of na+ ...	1983	6643194
[effect of pesticides: cartap and monocrotophos on the invasiveness of ova of ascaris suum and on the pathomorphological changes in the lung and liver of cavia porcellus with experimental ascariasis].		1983	6649636
cytoadherence effects of serum on ascaris suum infective larvae.	dead and live ascaris suum infective larvae were found to adhere to monolayer cultures of mouse peritoneal macrophages following incubation with normal rabbit serum (nrs) or with rabbit anti-a, suum (raas). cell-larval binding was shown to be affected by the numbers of larvae added to cell monolayers and by incubation time. larval adherence to cell monolayers was dependent upon the concentration of nrs or raas in the incubation mixture. dead larvae were not bound by macrophages in as significant ...	1983	6649985
ascaris-induced bronchoconstriction in primates experimentally infected with ascaris suum ova.	bronchial provocation with ascaris allergen evoked bronchoconstriction in home-bred macaca arctoides monkeys sensitized by experimental infection with embryonated ascaris suum ova. inhalation of ascaris allergen by aerosol prior to infection produced no changes in lung function. in infected animals total lung resistance (rl) increased and dynamic lung compliance (cdyn) decreased following ascaris inhalation. the changes in lung function reached a peak, 2-5 min after allergen inhalation, lasted f ...	1983	6652969
does atropine block antigen-induced contraction in canine bronchial rings? a preliminary report.	isometric contractions recorded with a strain gauge, were elicited with cumulative doses of ascaris suum antigen on fresh canine bronchial rings from 8 dogs. fifteen rings from 2 spontaneously sensitized dogs reacted with contractions to the antigen, while 11 rings from other dogs did not react. seven sensitized rings were re-challenged after atropine, which significantly prevented the antigen from contracting these rings at a time when antigen contracted 3 non-atropinized, simultaneously tested ...	1983	6653686
the localization of fumarase and malic enzyme in muscle mitochondria of ascaris suum.	the distribution of fumarase and malic enzyme in ascaris suum muscle mitochondria was investigated by employing digitonin fractionation techniques. the ability of these procedures to resolve the various submitochondrial fractions (intracristal space, inner membrane, matrix and inner membrane-matrix particles) was verified by electron microscopy and the distribution of appropriate marker enzymes. from the data obtained, it is concluded that fumarase in ascaris muscle mitochondria is located solel ...	1983	6656845
experimental ocular toxocara canis and ascaris suum infection: in vivo and in vitro study.		1983	6659031
hemodynamic mechanisms in anaphylaxis.	intravenous administration of ascaris suum antigen (1 mg) as well as histamine (5 mg) was associated with rapid and marked reductions in cardiac output (qt) and systemic arterial pressure (pa) in anesthetized mechanically ventilated dogs. when venous return was maintained constant by an external pump reservoir system, both antigen and histamine caused rapid reductions in the reservoir blood volume consistent with peripheral pooling. in addition, interruption of the splanchnic arterial inflow by ...	1983	6661813
response of bronchial smooth muscle to mast cell degranulation in situ.	we studied the response of bronchial smooth muscle to mast cell degranulation with ascaris suum antigen (aa) and compound 48/80 (48/80) in 26 mongrel dogs in situ. bronchial smooth muscle response was measured isometrically in situ from a segment of the right middle lobe bronchus; tracheal response was monitored isometrically as a control. after intra-arterial (ia) injection of aa into the bronchial circulation, bronchial contraction preceded tracheal contraction by 19.2 +/- 4.6 s (p less than 0 ...	1983	6662771
white spots of the liver in pigs experimentally infected with ascaris suum.	to make clear the relationship between ascaris suum infection and the appearance of white spot lesions on the surface of the liver in pigs, three groups of pigs were inoculated orally with embryonated a. suum eggs and observed clinicopathologically. group a of three pigs were inoculated 21 times with 100 eggs each of the nematode, group b of three pigs 4 times with 50,000 eggs each for 10 weeks, and group c of two pigs 2 times with 50,000 eggs each at a one-week interval. all the pigs were sacri ...	1983	6680775
pathological studies on white spots of the liver in fattening pigs.	hepatic lesions were found in fattening pigs derived from a farm where swine had been suffering from the multiple occurrence of white spots in the liver. they were examined at a slaughter-house for 18 months. the white spots were classified into three patterns on the basis of the macroscopic appearance; that is a compact, a mesh-worked, and a lymphonodular pattern. histologically, the following 3 kinds of lesions were seen: (1) eosinophilic interstitial hepatitis accompanied with intralobular ne ...	1983	6680776
the measurement of ascaris suum protein by radioimmunoassay in sera from patients with helminthiasis and with gastrointestinal diseases.	in this study, we have developed a reliable radioimmunoassay (ria) for ascaris suum protein (asp) and have measured the serum concentration of asp in control subjects and in patients with helminthiasis and a variety of gastrointestinal diseases. the cross-reactivities of asp antibody with other helminth worm antigens were also measured by ria. ascaris lumbricoides contained approximately the same amount of asp antigenic substance as did ascaris suum. toxocara canis had a high concentration of a ...	1983	6682964
nippostrongylus brasiliensis and ascaridia galli: mitochondrial respiration in free-living and parasitic stages.	aerobic respiratory pathways have been delineated and respiratory efficiency has been assessed in mitochondria isolated from embryonated eggs, infective larvae, and adult nippostrongylus brasiliensis and ascaridia galli. mitochondrial respiration in free-living stages of n. brasiliensis is mediated mainly by a mammalian-like antimycin a- and cyanide-sensitive pathway; specific respiratory activity is high and oxidative phosphorylation efficient. in mitochondria of adult n. brasiliensis, antimyci ...	1983	6683662
antigens in perienteric fluid of ascaris suum as detected through antibodies in pigs orally inoculated with fully embryonated eggs.	perienteric fluid (pf) of adult ascaris suum was fractionated by ammonium sulfate precipitation, gel filtration or deae--cellulose chromatography, and sucrose density gradient centrifugation. anti-sera (anti-ee) from pigs which were inoculated orally with fully embryonated eggs (ee) of a. suum were used in an indirect radioimmunoassay (iria) to determine which fractions of pf reacted positively in the analyses at the lowest protein concentration. these fractions were considered to contain more p ...	1983	6684353
anthelmintic activity of ivermectin in pigs naturally infected with ascaris and trichuris.	the anthelmintic efficacy of ivermectin administered by subcutaneous (sc) injection or orally via the drinking water was investigated in feeder pigs naturally infected with ascaris suum and trichuris suis. group 1 pigs (n = 5) were given drinking water as a placebo, group 2 pigs (n = 5) were given ivermectin by sc injection (300 micrograms/kg of body weight), and group 3 pigs (n = 5) were given ivermectin in water also at a dosage rate of 300 micrograms/kg of body weight. at necropsy 11 days lat ...	1983	6688706
effect of beta-adrenergic stimulation on experimental canine anaphylaxis in vivo.	we studied beta-adrenergic modulation of antigen-induced release of histamine and changes in lung function in natively allergic, anesthetized dogs. after thoracotomy and bilateral cervical vagotomy, samples of peripheral lung frozen in situ were obtained for measurement of adenosine cyclic 3',5'-monophosphate (camp) and histamine. in 11 dogs with beta-adrenergic blockade (propranolol), subsequent ascaris suum antigen (intravenous) decreased lung tissue histamine 21.5% +/- 5.3% (mean +/- se), wit ...	1983	6185556
induction of ige-mediated cutaneous, cellular, and airway reactivity in rhesus monkeys by ascaris suum infection.	in order to maintain a colony of rhesus monkeys with ige-mediated ascaris asthma, we evaluated the response of four animals to infection with ascaris suum ova. all four animals developed immediate-type cutaneous reactivity and airway responses. serum ige and igg antibody responses against ascaris antigen, as quantitated by the elisa, were seen in all animals. peripheral blood histamine release after stimulation with ascaris antigen was positive in all animals. airway reactivity to aerosol challe ...	1983	6189928
[attempt to isolate from ascaris suum an antigenic fraction for use in the immunologic diagnosis of onchocerciasis].	for the purpose of immunological diagnosis of onchocerciasis, the authors attempted to isolate an antigenic fraction from ascaris suum which would only detect anti-onchocerca volvulus antibodies. chromatofocusing was done on a crude antigenic preparation from the ascaris. immunoelectrophoresis showed that the fraction eluted between ph 5.6 and ph 4.9 contained antigens which reveal anti-onchocerca antibodies; anti-loa loa antibodies are revealed by the fraction eluted at ph less than 4. from the ...	1983	6192880
the effects of nifedipine and verapamil on antigen-induced bronchoconstriction in dogs.	the effects of calcium channel blockers, nifedipine and verapamil (i.v. and aerosol), were investigated in beagle dogs natively allergic to ascaris suum antigen. control exposures to an aerosol of ascaris antigen provoked significant bronchopulmonary changes, i.e., increases in pulmonary resistance (rl) and decreases in dynamic lung compliance (cdyn). pretreatment with either nifedipine or verapamil (200 micrograms/kg, i.v.) provided significant inhibition in the rl responses to ascaris antigen ...	1983	6194999
laboratory infection of primates with ascaris suum to provide a model of allergic bronchoconstriction.	wild-caught non-human primates are naturally sensitive to ascaris antigen and provide a useful model for studying atopic asthma. the present study was carried out to determine the effect of experimentally infecting home-bred macaques with the nematode ascaris suum and hence provide an alternative for the naturally occurring model. following oral infection with the parasite the animals developed a blood eosinophilia and specific antibodies to purified ascaris antigen. these antibodies appeared to ...	1983	6197218
activities of some enzymes in the perienteric fluid of ascaris suum.	activities of 12 enzymes (amylase, lipase, cholinesterase, nonspecific carboxyl esterase, lactate dehydrogenase (ldh), alkaline phosphatase, glutamate-oxalacetate transaminase (got), glutamate-pyruvate transaminase (gpt), gamma-glutamyl transferase (gamma-gt), leucine aminopeptidase (lap), malate dehydrogenase (mdh) and peroxidase) were determined in the perienteric fluid and homogenate of ascaris suum. with the exception of amylase, all activities were higher in the homogenate than in the perie ...	1983	6198263
clinical pathology of experimental stephanuriasis in pigs.	haematology and serum aspartate aminotransferase and alanine aminotransferase activities were studied during the migration phase of stephanurus dentatus in the livers of experimentally infected pigs. there was no evidence of anaemia but total leucocyte counts were raised and peripheral eosinophilia began 2 to 3 weeks after infection. peak eosinophilia occurred 6 to 7 weeks after infection and levels were still elevated at 20 weeks. lymphocyte and neutrophil numbers remained constant. reinfection ...	1983	6226277
preparation and characterization of a monoclonal anti-t helper factor antibody.	sprague-dawley rats were immunized by injection of antigen b-specific t helper factor thf) eluted from sepharose-antigen d adsorbents. rat spleen cells from animals immunized with thf were fused with a balb/c tumour cell (p3x63-ag8.653) to prepare monoclonal anti-thf antibodies. the hybrids produced were screened for anti-thf antibodies by an enzyme-linked immunoassay (elisa), and we shall describe the characteristics of one of the hybrid (hybridoma 6-2.2 anti-thf) antibodies produced. (i) the m ...	1984	6231242
ascaris suum: continuous perfusion of the pseudocoelom and nutrient absorption.	a double-cannulation apparatus was constructed for continuous perfusion of the pseudocoelom of adult ascaris suum while maintaining the intact parasite in a controlled incubation chamber. peristaltic pumps maintained a constant flow rate of artificial perienteric fluid through the incubation chamber (1 ml/min) and through the parasite (100 microliters/min). based on protein determinations, perienteric fluid was removed from the pseudocoelom within 35 min of initiation of perfusion (3.5 ml). a no ...	1984	6201385
indication of a certain molecular correlation between rat ige and human ige.	the pca-activity of rat sera sensitized with ascaris suum antigen can be absorbed by antihuman ige and by rabbit or sheep antihuman ige convalently bound to sephadex particles or paper discs respectively. in vitro, rat immune sera show positive results using the human rist-system abolished by preheating for 2 hours at 56 degrees c and therefore excluding an interaction of igg 4. in contrast, no rat sera activity was seen using the human prist-system. these results agree with the concept of a sin ...	1984	6205574
the role of slow-reacting substance of anaphylaxis in the late bronchial response after antigen challenge in allergic sheep.	allergic sheep respond to inhalation challenge with ascaris suum antigen with an immediate bronchoconstriction that resolves within 2 to 4 h, followed by a second bronchial obstruction occurring 6.5 to 8 h after challenge. the purpose of this study was to determine if the antigen-induced late bronchial response in allergic sheep is mediator dependent and to determine the extent to which histamine and/or slow-reacting substance of anaphylaxis (srs-a) are involved in this response. to do this, 10 ...	1984	6208832
late phase bronchial obstruction following nonimmunologic mast cell degranulation.	immunologic degranulation of airway mast cells after antigen inhalation produces early and late airway obstructions in allergic sheep. in this study we determined whether nonimmunologic degranulation of airway mast cells by inhalation of compound 48/80 had similar effects. in five sheep, pulmonary flow resistance (rl), thoracic gas volume (vtg), and arterial o2 tension (pao2) were determined prior to and at predetermined times after inhalation of 48/80 aerosol. immediately after challenge mean s ...	1984	6209256
[bronchoconstrictor reaction pattern of the upper respiratory tract and peripheral bronchial system].	ascaris suum hypersensitive dogs were exposed to ascaris aerosol, histamine, and acetylcholine at different time intervals separately to the upper part of the airtract and to the lower airways. before and during the exposure the plasma histamine concentrations were measured. acetylcholine and histamine delivered at the upper part of the airtract do not cause bronchoconstriction. histamine diffuses in relatively high amounts through the mucosa of the upper part of the airtract into the arterial p ...	1984	6463389
amino acid and lipid composition of refringent granules from the ameboid sperm of ascaris suum (nematoda).	transformation of the spermatozoon of ascaris suum from a spheroidal to an ameboid cell is associated with the formation of a motile pseudopodium and coalescence of the intracellular refringent granules. the pseudopodia of the ameboid spermatozoa contain filaments organized into dense patches, bundles, web-like or lace-like networks, as observed by electron microscopy. the morphology and chemistry of the refringent granules were investigated in subcellular fractions enriched for these structures ...	1984	6469722
the binding and subsequent inhibition of tubulin polymerization in ascaris suum (in vitro) by benzimidazole anthelmintics.	benzimidazole anthelmintics may act by interfering with the microtubule system in ascaris suum. binding of benzimidazole anthelmintics, and their inactive metabolites, to a. suum tubulin was demonstrated by the inhibition of intestinal extracts of the nematode to bind [3h]colchicine. in addition, these compounds inhibited the polymerization of tubulin into microtubules in vitro.	1984	6487354
parasitic liver damage in lambs with particular reference to the migrating larvae of ascaris suum.	it has been suggested that the larvae of the pig nematode ascaris suum is a possible cause of rejection of lambs' livers in the abattoir. however, this assertion was not substantiated in an experimental study in which lambs were dosed with infective a suum eggs. this produced, at the worst, small trivial lesions (up to 2 mm diameter) which would probably have been missed at meat inspection under commercial conditions. no evidence of ascarid lesions was observed in the livers of 5873 lambs and ho ...	1984	6495588
an ultraviolet-attenuated egg vaccine for swine ascariasis: parameters affecting the development of protective immunity.	pigs inoculated with uv-irradiated ascaris suum eggs developed immunity against challenge exposure with a suum infective eggs. trials were conducted to determine the optimal conditions necessary to induce high levels of protective immunity and minimize liver lesions related to larval migration. protective immunity was defined as the percentage of reduction in the recovery of larvae from the lungs of inoculated pigs, compared with controls, 7 days after challenge exposure with 5 x 10(3) or 10(4) ...	1984	6497109
vernal conjunctivitis. model studies in guinea pigs immunized topically with fluoresceinyl ovalbumin.	repeated topical applications of fluoresceinyl ovalbumin (fl-oa) to the conjunctival sac of guinea pigs sensitized them for conjunctival type 1 hypersensitivity reactions and mast cell degranulation. guinea pigs infected with ascaris suum, with high titers of circulating anti-a suum ige and igg1 antibody, also produced conjunctival type 1 reactions on topical challenge with a suum antigen. these reactions were no more intense than those of animals topically sensitized and challenged with fl-oa, ...	1984	6497752
[long-term effects of a deworming program using flubendazole and levamisole on the percentage of condemned livers in slaughtering pigs].	a deworming programme continued over a period of twenty-four months was carried out to study the proportion of livers condemned because of milk spots, which was unacceptably high on a pig-fattening farm in the province of limburg, the netherlands. in order to remove ascaris suum from the fattening pigs initially present and to prevent subsequent contamination of the houses with worm eggs, three courses of deworming treatment with flubendazole were initiated on the farm. all newly incoming piglet ...	1984	6506051
kinetic mechanism in the direction of oxidative decarboxylation for nad-malic enzyme from ascaris suum.	measurement of the initial rate of the malic enzyme reaction varying the concentration of nad at several different fixed levels of mg2+ (0.25-1.0 mm) and a single malate concentration gave a pattern which intersects to the left of the ordinate. repetition of this initial velocity pattern at several additional malate concentrations and treatment in terms of a terreactant mechanism suggests an ordered mechanism in which nad adds prior to mg2+ which must add prior to malate. on the other hand, when ...	1984	6509028
determination of dissociation constants for enzyme-reactant complexes for nad-malic enzyme by modulation of the thiol inactivation rate.	incubation of nad-malic enzyme from ascaris suum with the sulfhydryl reagents n-ethylmaleimide (nem), 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb), or 4,4'-dithiodipyridine (4-pds) results in rapid and complete loss of malate oxidative decarboxylase and pyruvate reductive carboxylase activities. with dtnb, this loss of activity occurs concomitantly with the modification of about 1 thiol group per subunit. the majority of the activity is lost when 0.5 thiol per subunit is modified, indicative of po ...	1984	6509029
immune response in rabbits experimentally infected with ascaris suum.	immune response of rabbits experimentally infected with ascaris suum was studied by indirect haemagglutination method. the animals were infected with the doses of 5,000, 10,000 and 20,000 infective eggs per animal. positive reactions were observed from days 5-11 p.i., maximum reactions on days 11-19 p.i. a reinfection with the same doses (1x or 2x after 35 and 65 days) increased the antibody titre. the strongest individual reaction was recorded on day 19 p.i. in the group infected with the highe ...	1984	6510836
performance and tissue copper concentrations of control and ascaris suum-infected pigs fed excess dietary copper.	ninety-eight crossbred growing-finishing swine were used in 2 experiments to investigate the interaction between naturally occurring ascaris suum infections and excess copper ingestion. in both experiments, pigs were fed a 14% crude protein basal diet or the basal diet + 250 ppm copper. these 2 dietary groups were subsequently divided into 2 additional treatment groups (a. suum-infected or uninfected) based on worm recovery from the intestine or from fecal egg counts. excess dietary copper impro ...	1984	6512632
electron transfer complexes of ascaris suum muscle mitochondria: i. characterization of nadh-cytochrome c reductase (complex i-iii), with special reference to cytochrome localization.	an nadh-cytochrome c reductase (complex i-iii) was isolated from ascaris suum muscle mitochondria. the enzyme preparation catalyzed the reduction of 1.68 mumol cytochrome c min-1 mg-1 protein at 25 degrees c with nadh but not with nadph, and retained its sensitivity to rotenone, piericidin a and 2-heptyl-4-hydroxyquinoline-n-oxide as with the submitochondrial particles. the isolated complex i-iii, essentially free of succinate-cytochrome c reductase and cytochrome c oxidase, consisted of fourtee ...	1984	6513990
the fumarate reductase system as a site of anthelmintic attack in ascaris suum.	various benzimidazole compounds have been shown to be highly effective as inhibitors (up to 50% reduction of activity) in vitro of the helminth-specific enzyme, fumarate reductase, of ascaris suum. anthelmintically active and inactive benzimidazoles were similarly effective as inhibitors of enzyme activity. albendazole-induced inhibition of fumarate reductase was not observed when the enzyme was preincubated with nadh.	1984	6518278
culture requirements of ascaris suum larvae using a stationary multi-well system: increased survival, development and growth with cholesterol.	ascaris suum third-stage larvae (l3) were recovered from infected rabbits and cultured in a stationary multi-well plate system. several different culture media including medium 199, nctc-135, minimum essential medium-eagle, dulbecco's modified eagle's medium, rpmi 1640, mccoy's 5a and neuman-tytell medium were tested to determine which was best for overall larval survival, development and growth. larvae developed from l3 to fourth-stage (l4) in all media tested, but larval survival and the yield ...	1984	6538364
antibody responses to toxocara canis using sera from parasite-infected mice and protection from toxocariasis by immunisation with es antigens.	evidence for serological cross-reactions between toxocara canis and related nematode parasites has been sought using sera from infected mice and the immunodiagnostic excretory/secretory (es) antigen of t. canis larvae. sera from mice experimentally infected with either t. canis, t. cati, t. pteropodis, toxascaris leonina or ascaris suum were tested for the presence of antibodies to t. canis es antigen by solid-phase radioimmunoassay (ria) or enzyme-linked immunosorbent assay (elisa) using anti-m ...	1984	6543490
comparison of counterimmunoelectrophoresis with indirect haemagglutination test in the detection of antibodies in rabbits experimentally infected with various species of ascarids.	counterimmunoelectrophoresis (ciep) and indirect haemagglutination (iha) were used for the detection of antibodies in 437 sera of rabbits experimentally infected with ascaris suum, toxocara canis and toxascaris leonina. ciep showed 44.0-52.3% positivity in a. suum, 25.5% in t. canis and 19.7-29.2% in t. leonina infection, whereas the positivity detected by iha was 59.8-90.2% in a. suum, 44.8% in t. canis and 59.6% in t. leonina infection. a comparison of the two tests reveals that ciep is a suit ...	1984	6589203
isolation and properties of alpha-amylase from perienteric fluid of ascaris suum.	the properties of alpha-amylase from perienteric fluid of ascaris suum were studied. the enzyme is strongly activated by chlorides at a simultaneous shift of ph optimum. it is fully activated already at 10(-3) m concentration of chlorides. a method of alpha-amylase isolation was modified with respect to these properties to give the purification degree 278 and yield of 11%.	1984	6611287
some experimental approaches to the study of nutrition and parasitic infection.	two experimental models to examine the relationship between host nutrition and parasitic infection are considered. moniliformis dubius, an acanthocephalan parasite of the laboratory rat, is dependent on the type and amount of host dietary carbohydrate for growth and reproduction. starch, glucose, fructose, and mannose in host diets support parasite growth and development, but much lower dietary amounts of starch, fructose, and mannose are needed compared to free glucose. the pig-ascaris suum rel ...	1984	6692943
nadh-dependent tiglyl-coa reduction in disrupted mitochondria of ascaris suum.	the incubation of 2-methylcrotonyl-coa with either succinate or nadh and disrupted ascaris suum mitochondria results in substantial 2-methylbutyrate formation. both membrane-bound and soluble components are required and the nadh-dependent reduction is rotenone sensitive, suggesting the involvement of the electron-transport chain. rat liver mitochondria, incubated under similar conditions, did not catalyze 2-methylbutyrate formation. however, the substitution of a. suum mitochondrial membranes fo ...	1984	6694671
use of a rast technique with ascaris suum allergen to detect a. lumbricoides infestation.		1984	6701708
2-methylbutyryl coa dehydrogenase from mitochondria of ascaris suum and its relationship to nadh-dependent 2-methylcrotonyl coa reduction.	acyl coa dehydrogenase and electron-transfer flavoprotein have been isolated and partially purified from mitochondria of the anaerobic nematode, ascaris suum. dehydrogenase activity was greatest with 2-methylbutyryl coa and the relative substrate specificities of the ascarid dehydrogenase(s) differ greatly from their mammalian counterparts. it appears that the ascarid dehydrogenase functions physiologically as a reductase, catalyzing the final step in the synthesis of branched-chain fatty acids. ...	1984	6704106
changes in the somatic musculature of the 4th-stage larvae of ascaris suum goeze, 1782 (nematoda: ascaridoidea) developing in swine.	fourth-stage larvae of ascaris suum, recovered from piglets at 11-22 days post-infection (p.i.), were examined by light microscopy for changes in the somatic musculature. during this time the estimated total number of muscle cells in the body increases from approximately 600 to 21 000 cells. this non-eutelic development appears to occur by division of platymyarian muscle cells into coelomyarian cells, thereby increasing the number of muscle cells/quadrant from 5 to 85. the incompleteness of thes ...	1984	6709390