Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| inulinase production by kluyveromyces marxianus nrrl y-7571 using solid state fermentation. | inulinase is an enzyme relevant to fructose production by enzymatic hydrolysis of inulin. this enzyme is also applied in the production of fructo-oligosaccharides that may be used as a new food functional ingredient. commercial inulinase is currently obtained using inulin as substrate, which is a relatively expensive raw material. in brazil, the production of this enzyme using residues of sugarcane and corn industry (sugarcane bagasse, molasses, and corn steep liquor) is economically attractive, ... | 2006 | 16915703 |
| optimization of lactose utilization in deproteinated whey by kluyveromyces marxianus using response surface methodology (rsm). | kluyveromyces marxianus y-8281 yeast culture was utilized for the biological treatment of deproteinated whey wastewater in a batch system. removal of lactose was optimized by the utilization of response surface methodology, rsm. the empirical model developed through rsm in terms of effective operational factors of medium ph, temperature, lactose and ammonia concentrations was found adequate to describe the treatment of deproteinated whey. through the analysis, medium ph and temperature were foun ... | 2006 | 16364636 |
| yeast ecology in french cider and black olive natural fermentations. | in this study, rdna its restriction analysis was used to identify yeasts from two naturally fermented products: french ciders and black olives. in cider musts and bottled ciders, the pcr-rflp method generated 15 different its/rflp profiles for a total of 208 isolates. the predominant yeasts corresponded to saccharomyces bayanus, saccharomyces cerevisiae, lachancea cidri and dekkera anomala. three identified species: candida sake, candida tropicalis and kluyveromyces marxianus had never been desc ... | 2006 | 16380183 |
| antimicrobial properties of lactic acid bacteria and yeast-lab cultures isolated from traditional fermented milk against pathogenic escherichia coli and salmonella enteritidis strains. | the survival and growth of escherichia coli 3339 and salmonella enteritidis 949575 isolated from human clinical samples, in milk fermented with lactic acid bacteria (lab) and yeast strains previously isolated from zimbabwean naturally fermented milk (nfm) was studied. the lab starter cultures used were lactococcus lactis subsp. lactis biovar. diacetylactis c1 alone (c1) or in combination with candida kefyr 23 (c1/23), l. lactis subsp. lactis lc261 alone (lc261) or in combination with c. kefyr 23 ... | 2006 | 16387379 |
| in vitro effects of micafungin against candida biofilms on polystyrene and central venous catheter sections. | long-term inserted and surgically implanted catheters can be colonised by candida spp. candida biofilms in vitro are often resistant to antifungal agents. the aim of this study was to investigate the in vitro activity of micafungin (mfg) against six candida spp. biofilms on polystyrene (ps) and central venous catheter (cvc) sections. safranin staining and differential interference contrast microscopy were used to demonstrate biofilm production. mfg activity was determined by the reduction in met ... | 2006 | 17101265 |
| the crystal and solution structures of glyceraldehyde-3-phosphate dehydrogenase reveal different quaternary structures. | the presence of an isoform of glyceraldehyde-3-phosphate dehydrogenase (kmgapdh1p) associated with the cell wall of a flocculent strain of kluyveromyces marxianus was the first report of a non-cytosolic localization of a glycolytic enzyme, but the mechanism by which the protein is transported to the cell surface is not known. to identify structural features that could account for the multiple localizations of the protein, the three-dimensional structure of kmgapdh1p was determined by x-ray cryst ... | 2006 | 16963457 |
| a reverse transcriptase pcr technique for the detection and viability assessment of kluyveromyces marxianus in yoghurt. | a fast and sensitive reverse transcriptase pcr (rt-pcr) method was developed for the detection of viable kluyveromyces marxianus in yoghurt. yeast-specific primers were used with the rt-pcr to evaluate the suitability of 18s rrna as a target for the detection of viable yeasts in pure culture and yoghurt. the rt-pcr assay was able to detect down to 10(2) cfu ml(-1) in yoghurt samples contaminated with viable yeast cells. application of the rt-pcr method to commercial yoghurt samples demonstrated ... | 2006 | 16995526 |
| growth characteristics of fusants by protoplast fusion between the thermotolerant yeast, kluyveromyces marxianus, and the starch-assimilating yeast, schwanniomyces occidentalis. | intergeneric fusants were obtained by protoplast fusion between the thermotolerant yeast, kluyveromyces marxianus, and the starch-assimilating yeast, schwanniomyces occidentalis. two thermotolerant fusants growing at 40 degrees c were screened on the medium containing soluble starch. these fusants showed weak growth in a soluble starch medium and the production of a little amylase. the carbon source assimilation and the chromosome composition of the fusants were similar to those of the k. marxia ... | 2006 | 16789551 |
| kluyveromyces lactis and saccharomyces cerevisiae, two potent deacidifying and volatile-sulphur-aroma-producing microorganisms of the cheese ecosystem. | cheese flavour is the result of complex biochemical transformations attributed to bacteria and yeasts grown on the curd of smear-ripened cheeses. volatile sulphur compounds (vscs) are responsible for the characteristic aromatic notes of several cheeses. in the present study, we have assessed the ability of kluyveromyces lactis, kluyveromyces marxianus and saccharomyces cerevisiae strains, which are frequently isolated from smear-ripened cheeses, to grow and deacidify a cheese medium and generate ... | 2006 | 16847604 |
| is candida kefyr an emerging pathogen in patients with oncohematological diseases? | 2006 | 16886166 | |
| asymmetric reduction of o-chloroacetophenone with candida pseudotropicalis 104. | the asymmetric reduction of o-chloroacetophenone 1 with candida pseudotropicalis 104 produced the corresponding (s)-1-(2-chloro-phenyl)-ethanol 2 with the enantiomeric excess (ee >99%) without addition of any cosolvent. the cell could tolerate high ketone 1 concentration of 233.8 mmol/l (i.e., 36 g/l) with considerable reduction activity in this method. the product 2 concentration achieved 38.9 and 58.4 mmol/l with cells of 40 and 60 g(dcw) (dry cell weight)/l, respectively, in 24 h. the optimum ... | 2006 | 17022667 |
| an aqueous-organic two-phase bioprocess for efficient production of the natural aroma chemicals 2-phenylethanol and 2-phenylethylacetate with yeast. | the natural aroma chemicals 2-phenylethanol (2-pe) and 2-phenylethylacetate (2-peac) are of high industrial relevance and can be produced from l-phenylalanine in a yeast-based process with growth-associated product formation. due to product inhibition, in situ product removal is mandatory to obtain economically interesting concentrations. a fed-batch approach using polypropylene glycol 1200 as in situ extractant and the precursor in a saturated concentration led to the highest 2-pe productivity ... | 2006 | 16397768 |
| application of sscp-pcr fingerprinting to profile the yeast community in raw milk salers cheeses. | bacteria and yeasts are important sensory factors of raw-milk cheeses as they contribute to the sensory richness and diversity of these products. the diversity and succession of yeast populations in three traditional registered designation of origin (r.d.o.) salers cheeses have been determined by using phenotypic diagnoses and single-strand conformation polymorphism (sscp) analysis. isolates were identified by phenotypic tests and the sequencing of the d1-d2 domains of the 26s rrna gene. ninety- ... | 2006 | 16464699 |
| robustness of cascade ph and dissolved oxygen control in symbiotic nisin production process system of lactococcus lactis and kluyveromyces marxianus. | in symbiotic processes, different organisms coexist stably and interact by sharing the same metabolites and environmental conditions. the robustness of a symbiotic nisin production process system composed of the lactic acid bacterium lactococcus lactis subsp. lactis (atcc11454) and dairy yeast kluyveromyces marxianus (ms1) was studied. it was found that this symbiotic process system was robust to the initial disturbance in the inoculum sizes of both microorganisms and ph. | 2006 | 16716931 |
| application of fluorescence in situ hybridisation (fish) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations. | to analyse the yeast population diversity during wine fermentations, specific fluorescein-labelled oligonucleotide probes targeted to the d1/d2 region of the 26s rrna of different yeast species known to occur frequently in this environment were designed and tested with reference strains. the probes were then used to identify wine must isolates and to follow, in combination with plate counts, the evolution of yeast populations in two winery fermentations of white and red grape musts. in both case ... | 2006 | 16504329 |
| novel lysine biosynthetic gene sequences (lys1 and lys5) used as pcr targets for the detection of the pathogenic candida yeast. | we report here a sensitive and specific polymerase chain reaction (pcr) detection assay for the pathogenic candida yeast based on the novel lys1 [encoding saccharopine dehydrogenase (sdh)] and lys5 [encoding phosphopantetheinyl transferase (pptase)] gene sequences of the fungal unique lysine biosynthetic pathway. both lys1 and lys5 dna-specific pcr primers sg1, sg2 and sg3, sg4, respectively, amplified predicted 483 and 648-bp fragments from candida albicans genomic dna but not from other select ... | 2006 | 16733732 |
| [prevalence of vaginal candidiasis in pregnant women. identification of yeasts and susceptibility to antifungal agents]. | pregnant women are more susceptible to both vaginal colonization and infection by yeast. our objectives were to determine the prevalence in pregnant women of yeasts isolated from vaginal exudates and their susceptibility to current antifungal drugs. a total of 493 patients was studied between december 1998 and february 2000. the prevalence of candida spp. was 28% (candida albicans 90.4%; candida glabrata 6.3%; candida parapsilosis 1.1%, candida kefyr 1.1 %; unidentified species 1.1 %). the diffu ... | 2006 | 16784126 |
| pectin degrading enzymes in yeasts involved in fermentation of coffea arabica in east africa. | the ability of six strains of pichia anomala, four strains of pichia kluyveri and two strains of hanseniaspora uvarum predominant during coffee processing to produce polygalacturonase (pg), pectin esterase (pe) and pectin lyase (pl) in yeast polygalacturonic acid medium (ypa) and in coffee broth (cb) was studied. for comparison, a reference strain of kluyveromyces marxianus cct 3172 isolated from cocoa and reported to produce high amount of pg was included. initial screening of pg activity using ... | 2006 | 16784790 |
| antimicrobial and antioxidant activities of some nigerian medicinal plants. | ten nigerian plants suggested from their ethnomedical uses to possess antimicrobial and antioxidant activities were studied for their anti-microbial and anti-oxidant properties. antimicrobial activity was tested against escherichia coli nctc 10418, pseudomonas aeruginosa, staphylococcus aureus, bacillus subtilis, candida albicans, candida pseudotropicalis and trichophyton rubrum (clinical isolate). trichilia heudelotti leaf extract showed both antibacterial and antifungal activities and was the ... | 2006 | 20162089 |
| the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species. | in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ... | 2007 | 17761478 |
| correlation of posaconazole minimum fungicidal concentration and time kill test against nine candida species. | we evaluated the in vitro activity of posaconazole against nine candida species using minimum fungicidal concentration (mfc) measurements and time-kill methods. | 2007 | 17875607 |
| new ethacridine derivatives as the potential antifungal and antibacterial preparations. | until the 20th century fungal infections were rather easy cured, and the need of new antifungal drugs was low. however, low choice of antifungal preparations, their toxicity, limited spectrum of action, and ability to produce resistant strains show the need of new effective medicines for systemic fungal diseases in nowadays. our goal of research was to synthesize new antimicrobial compounds containing three or more pharmacophores in one molecule. the initial 5-substituted-2-methylmercaptothiazol ... | 2007 | 17895642 |
| mixed cultures of serratia marcescens and kluyveromyces fragilis for simultaneous protease production and cod removal of whey. | the aim of this study was to investigate the behaviour of a serratia marcescens-kluyveromyces fragilis mixed culture in whey, with the objective of proteases production and organic waste reduction. | 2007 | 17897188 |
| lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin. | to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development. | 2007 | 17650194 |
| evaluation of albicans id2 and biggy agar for the isolation and direct identification of vaginal yeast isolates. | in this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, albicans id(2) and biggy agar, as well as onto sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. these strains were identified as candida glabrata in 20 (31.8 %) samples, candida albicans in 15 samples (23.8 %), candida tropicalis in 10 samples (15.9 %), candida krusei in five samples (7.9 %), candida kefyr in five samples (7.9 %), cand ... | 2007 | 17510260 |
| ce separation of proteins and yeasts dynamically modified by peg pyrenebutanoate with fluorescence detection. | the optimized protocols of the bioanalytes separation, proteins and yeasts, dynamically modified by the nonionogenic tenside peg pyrenebutanoate, were applied in cze and cief with the acidic gradient in ph range 2-5.5, both with fluorescence detection. peg pyrenebutanoate was used as a buffer additive for a dynamic modification of proteins and/or yeast samples. the narrow peaks of modified analytes were detected. the values of the pi's of the labeled proteins were calculated using new fluorescen ... | 2007 | 17557360 |
| epidemiology and antifungal susceptibilities of candida spp. to six antifungal agents: results from a surveillance study on fungaemia in germany from july 2004 to august 2005. | data on fungal infections occurring in germany are rare to date. the aim of the present study was to survey the epidemiological situation in germany, to provide data on the susceptibility of the fungal isolates to antifungals. | 2007 | 17562683 |
| comparison of yeast strains for batch ethanol fermentation of cheese-whey powder (cwp) solution. | to test the suitability of cheese whey powder (cwp) solution for ethanol fermentation and to compare performances of different kluyveromyces marxianus strains for ethanol fermentation from cwp solution. | 2007 | 17576220 |
| construction of thermotolerant yeast expressing thermostable cellulase genes. | kluyveromyces marxianus nbrc1777 was identified as a thermotolerant yeast and was developed as a host for the expression of thermostable cellulase genes. the previously isolated genes for thermostable endo-beta-1,4-glucanase, cellobiohydrolase, and beta-glucosidase were introduced into the chromosome of k. marxianus and successfully expressed under the control of high-expression promoters. the recombinant k. marxianus expressing cellulase genes became able to grow in synthetic medium containing ... | 2007 | 17433483 |
| optimization of medium and process parameters for the production of inulinase from a newly isolated kluyveromyces marxianus ys-1. | a newly isolated strain of kluyveromyces marxianus ys-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, cacl2 and sodium dodecyl sulphate (sds). fermentation medium ph 6.5, cultivation temperature 30 degrees c and 5% (v/v) inoculum of 12 h-old culture were optimal for enzyme production (30.8 iu/ml) with a fermentation time of 72 h at shake flask level. raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15 kg/cm2 for 10 min was ... | 2007 | 17071081 |
| fermentative production of superoxide dismutase with kluyveromyces marxianus. | this work sought to develop a fermentative process for the microbial production of superoxide dismutase (sod), to overcome extraction from animal tissues. twenty-eight wild-type yeast strains were screened for sod productivity. kluyveromyces marxianus l3 showed the highest sod activity (62 u mg(-1)) and was used for process development. oxidative stress conditions and parameters affecting oxygen transfer rate were exploited to improve production. the effects of dilution rate (0.067 vs 0.2 h(-1)) ... | 2007 | 16909269 |
| partial purification and characterization of exoinulinase from kluyveromyces marxianus ys-1 for preparation of high-fructose syrup. | an extracellular exoinulinase (2,1-beta-d fructan fructanohydrolase, ec 3.2.1.7), which catalyzes the hydrolysis of inulin into fructose and glucose, was purified 23.5-fold by ethanol precipitation, followed by sephadex g-100 gel permeation from a cell-free extract of kluyveromyces marxianus ys-1. the partially purified enzyme exhibited considerable activity between ph 5 to 6, with an optimum ph of 5.5, while it remained stable (100%) for 3 h at the optimum temperature of 50 degrees c. mn2+ and ... | 2007 | 18051293 |
| kinetics of batch ethanol fermentation of cheese-whey powder (cwp) solution as function of substrate and yeast concentrations. | a lactose utilizing yeast strain, kluyveromyces marxianus dsmz-7239 was used for ethanol formation from cheese-whey powder (cwp) solution in batch experiments. effects of initial substrate (cwp) and yeast concentrations on the rate and extent of ethanol formation were investigated. the initial ph and oxidation-reduction potential (orp) was kept at 5 and -250 mv, respectively. the rate and extent of ethanol formation increased with increasing cwp concentration up to 156 g l(-1) (75 g l(-1) sugar) ... | 2007 | 17118651 |
| kinetic parameters and thermodynamic values of beta-xylosidase production by kluyveromyces marxianus. | kinetic parameters for production of beta-xylosidase by kluyveromyces marxianus were determined in growth media containing glucose, xylose, cellobiose, sucrose and lactose as carbon sources. k. marxianus achieved maximum beta-xylosidase specific product yield (y(p/x)) when grown on xylose. basal level of activity was achieved in cultures grown on glucose. kinetic parameters of enzyme production and cell mass formation were correlated. enzyme synthesis was regulated by an induction mechanism and ... | 2007 | 17098425 |
| [identification and biodiversity of yeasts isolated from koumiss in xinjiang of china]. | a total of 87 yeast strains were isolated from 28 home-made koumiss samples, a traditional fermented mare milk product in xinjiang of china. the isolates were identified by standard physiological and biochemical tests and analysis of the large-subunit (26s) rdna gene d1/d2 domain sequences. they are proved to be saccharomyces unisporus (48.3% of the isolates), kluyveromyces marxianus (27.6%) and pichia membranaefaciens (15.0%), saccharomyces cerevisiae (9.2%). among them, six isolates and a stan ... | 2007 | 17944353 |
| molecular characterization of kluyveromyces marxianus strains isolated from agave fourcroydes (lem.) in yucatan, mexico. | the molecular characterization of 14 strains of kluyveromyces marxianus isolated from agave fourcroydes (lem.) in yucatan, mexico, was performed by ap-pcr analysis, pcr-rflp of 5.8s-its, and complete nts regions. a sequence analysis of the d1/d2 domain of the 26s rdna was also carried out in six selected strains. the ap-pcr approach had the highest discrimination power for the molecular characterization of new henequen k. marxianus strains. pcr-rflp of 5.8s-its regions did not reveal polymorphis ... | 2007 | 17952662 |
| continuous ethanol fermentation of cheese whey powder solution: effects of hydraulic residence time. | continuous ethanol fermentation of cheese whey powder solution was realized using pure culture of kluyveromyces marxianus (dsmz 7239) at hydraulic residence times (hrt) between 12.5 and 60 h. sugar utilization, ethanol and biomass formation were investigated as functions of hrt. effluent sugar concentration decreased, but percent sugar utilization, ethanol and biomass concentrations increased with hrt. ethanol productivity was maximum (0.745 ge l(-1)h(-1)) at an hrt of 43.2 h where the biomass p ... | 2007 | 17143639 |
| synthesis, characterization and antimicrobial activity of fe(ii), zn(ii), cd(ii) and hg(ii) complexes with 2,6-bis(benzimidazol-2-yl) pyridine ligand. | 2,6-bis(benzimidazol-2-yl)pyridine (l) ligand and complexes [m(l)cl(2)] and [fe(l)(2)](clo(4))(2) (m=zn, cd, hg) have been synthesized. the geometries of the [m(l)cl(2)] complexes were derived from theoretical calculation in dgauss/dft level (dzvp basis set) on cache. the central m(ii) ion is penta-coordinated and surrounded by n(3)cl(2) environment, adopting a distorted trigonal bipyramidal geometry. the ligand is tridentate, via three nitrogen atoms to metal centre and two chloride ions lie on ... | 2007 | 17189664 |
| identification and characterization of yeast isolated from the elaboration of seasoned green table olives. | the purpose of this study was to investigate the yeast population during the processing of green table olives. in the fresh olives, yeast were found at concentrations of around 3.0 log cfu/g, with cryptococcus spp. being predominant. in the brine, the yeast concentrations were greater than 4.9 log cfu/ml, with pichia anomala, kluyveromyces marxianus, and saccharomyces cerevisiae being the predominant species. unlike the yeast isolated from the fresh olives, the strains obtained from the olive br ... | 2007 | 17189760 |
| physiology of the yeast kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source. | growth, substrate consumption, metabolite formation, biomass composition and respiratory parameters of kluyveromyces marxianus atcc 26548 were determined during aerobic batch and chemostat cultivations, using mineral medium with glucose as the sole carbon source, at 30 degrees c and ph 5.0. carbon balances closed within 95-101% in all experiments. a maximum specific growth rate of 0.56 h(-1), a biomass yield on glucose of 0.51 g g(-1), and a maximum specific consumption of oxygen of 11.1 mmol g( ... | 2007 | 17233766 |
| modelling thermal stability and activity of free and immobilized enzymes as a novel tool for enzyme reactor design. | in this work, a novel method is proposed to establish the most suitable operational temperature for an enzyme reactor. the method was based on mathematical modelling of the thermal stability and activity of the enzyme and was developed using thermodynamic concepts and experimental data from free and immobilized inulinases (2,1-beta-d fructan frutanohydrolase, ec 3.2.1.7) from kluyveromyces marxianus, which were used as examples. the model was, therefore, designed to predict the enzyme activity w ... | 2007 | 17254780 |
| the influence of a pulsating electric field on selenium accumulation in kluyveromyces marxianus cells. | k. marxianus was cultivated under conditions of pef (pulse electric field) action and a selenium source. the culture duration after which cells were treated with pef, the field exposure time and the selenium concentration in the medium were all optimized. optimization of culture duration caused a 33% increase in selenium accumulation in cells as compared to the control with no pef treatment. the highest selenium accumulation--about 167 microg/g dry mass (dm)--was recorded after 3-minute pef trea ... | 2007 | 17304619 |
| yeast pyruvate decarboxylases: variation in biocatalytic characteristics for (r)-phenylacetylcarbinol production. | based on previous studies, candida utilis pyruvate decarboxylase (pdc) proved to be a stable and high productivity enzyme for the production (r)-phenylacetylcarbinol (pac), a pharmaceutical precursor. however, a portion of the substrate pyruvate was lost to by-product formation. to identify a source of pdc which might overcome this problem, strains of four yeasts -- c. utilis, candida tropicalis, saccharomyces cerevisiae and kluyveromyces marxianus -- were investigated for their pdc biocatalytic ... | 2007 | 17311582 |
| effect of kluyveromyces marxianus yit 8292 crude cell wall fraction on serum lipids in normocholesterolemic and mildly hypercholesterolemic subjects. | the hypocholesterolemic effects of kluyveromyces marxianus yit 8292 crude cell wall (km-cw) were examined. in pilot studies, km-cw tablets were administered to mildly hypercholesterolemic subjects at doses of 8.0, 4.0, 2.0, or 1.0 g/d for 4 weeks. total cholesterol (tc) and low-density lipoprotein cholesterol (ldl-c) decreased at doses above 2.0 and 4.0 g/d, respectively. further, we examined the effect of intake of yogurt containing 3.0 or 4.0 g of km-cw/d for 8 weeks in normal and hypercholest ... | 2007 | 17420604 |
| comparison of the gene expression patterns of alcohol dehydrogenase isozymes in the thermotolerant yeast kluyveromyces marxianus and their physiological functions. | four genes encoding alcohol dehydrogenase (adh) isozymes in the thermotolerant yeast kluyveromyces marxianus, a potent candidate for ethanol production at high temperatures, were investigated. of these, kmadh3 and kmadh4 were cloned and sequenced, and their deduced amino acid sequences were compared with those of kmadh1 and kmadh2 and other adhs of kluyveromyces lactis and saccharomyces cerevisiae. the four kmadhs had high sequence similarity, though kmadh3 and kmadh4 possessed an amino-terminal ... | 2007 | 17485854 |
| production of fuel ethanol at high temperature from sugar cane juice by a newly isolated kluyveromyces marxianus. | kluyveromyces marxianus dmku 3-1042, isolated by an enrichment technique in a sugar cane juice medium supplemented with 4% (w/v) ethanol at 35 degrees c, produced high concentrations of ethanol at both 40 and 45 degrees c. ethanol production by this strain in shaking flask cultivation in sugar cane juice media at 37 degrees c was highest in a medium containing 22% total sugars, 0.05% (nh(4))(2)so(4), 0.05% kh(2)po(4), and 0.15% mgso(4).7h(2)o and having a ph of 5.0; the ethanol concentration rea ... | 2007 | 17537627 |
| purification and properties of a beta-galactosidase with potential application as a digestive supplement. | functional-based screening of crude beta-galactosidase activities from 42 yeast strains resulted in the selection of a single enzyme of potential interest as a digestive supplement. beta-galactosidase produced by kluyveromyces marxianus dsm5418 was purified to homogeneity by a combination of gel filtration, ion-exchange, and hydroxylapatite chromatographies. the denatured (123 kda) and native molecular masses (251 kda) suggest that the enzyme is a homodimer. the optimum ph and temperature of the ... | 2007 | 17625262 |
| production of high fructose syrup from asparagus inulin using immobilized exoinulinase from kluyveromyces marxianus ys-1. | extracellular exoinulinase from kluyveromyces marxianus ys-1, which hydrolyzes inulin into fructose, was immobilized on duolite a568 after partial purification by ethanol precipitation and gel exclusion chromatography on sephadex g-100. optimum temperature of immobilized enzyme was 55 degrees c, which was 5 degrees c higher than the free enzyme and optimal ph was 5.5. immobilized biocatalyst retained more than 90% of its original activity after incubation at 60 degrees c for 3 h, whereas in free ... | 2007 | 17665236 |
| application of the cre-loxp system for multiple gene disruption in the yeast kluyveromyces marxianus. | the yeast kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. in order to take full advantage of this yeast and its particular properties, proper tools for gene disruption and metabolic engineering are needed. the cre-loxp system is a very versatile tool that allows for gene marker rescue, resulting in mutant strains free of exogenous selective markers, which is a very important aspect for indu ... | 2007 | 17624462 |
| gene expression and biochemical analysis of cheese-ripening yeasts: focus on catabolism of l-methionine, lactate, and lactose. | dna microarrays of 86 genes from the yeasts debaryomyces hansenii, kluyveromyces marxianus, and yarrowia lipolytica were developed to determine which genes were expressed in a medium mimicking a cheese-ripening environment. these genes were selected for potential involvement in lactose/lactate catabolism and the biosynthesis of sulfur-flavored compounds. hybridization conditions to follow specifically the expression of homologous genes belonging to different species were set up. the microarray w ... | 2007 | 17308183 |
| sequential gene integration for the engineering of kluyveromyces marxianus. | the attributes of the yeast kluyveromyces marxianus (rapid growth rate at high temperature, utilization of a wide range of inexpensive carbon sources) make it a promising industrial host for the synthesis of protein and non-protein products. however, no stable multicopy plasmids are currently available for long-term culture of k. marxianus. to allow the stable genetic/metabolic engineering of k. marxianus, a method for integrating precise numbers of the same or different genes was developed for ... | 2007 | 16982106 |
| iron enriched yeast biomass--a promising mineral feed supplement. | yeast biomass enriched with iron could represent a new and safer solution for prevention from anaemia development. such an iron source is less toxic and has better absorbability in organisms. the purpose of our research was the determination of the most suitable iron source in the cultivation medium for the yeast saccharomyces cerevisiae, regarding good growth and iron accumulation in cells. iron(iii) citrate, iron(iii) chloride, iron(iii) nitrate and fe-edta complex were used. the uptake of the ... | 2007 | 16935492 |
| purification and characterization of the enantioselective esterase from kluyveromyces marxianus cbs 1553. | an intracellular esterase from the yeast kluyveromyces marxianus cbs 1553 with interesting enantioselective hydrolytic activity towards racemic esters of 1,2-o-isopropylidene glycerol (ipg) was purified and characterized. optimal culture conditions for the obtainment of the enantioselective esterase on a 5 l-fermentation scale were investigated. two esterase activities (est1 and est2) in the crude cell extract were identified by native page with specific activity staining and separated from each ... | 2008 | 18029043 |
| characterization of the superoxide dismutase sod1 gene of kluyveromyces marxianus l3 and improved production of sod activity. | superoxide dismutase (sod) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. the cu/zn superoxide dismutase gene (kmsod1) of kluyveromyces marxianus l3 was cloned and characterized. the deduced kmsod1p protein shares 86% and 71% of identity with kluyveromyces lactis and saccharomyces cerevisiae sod1p, respectively. the characteristic motifs and the amino acid residues involved in coordinating ... | 2008 | 18040680 |
| development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin. | a 23.5-fold purified exoinulinase with a specific activity of 413 iu/mg and covalently immobilized on duolite a568 has been used for the development of a continuous flow immobilized enzyme reactor for the hydrolysis of inulin. in a packed bed reactor containing 72 iu of exoinulinase from kluyveromyces marxianus ys-1, inulin solution (5%, ph 5.5) with a flow rate of 4 ml/h was completely hydrolyzed at 55 degrees c. the reactor was run continuously for 75 days and its experimental half-life was 72 ... | 2008 | 18389297 |
| yeast biomass production in brewery's spent grains hemicellulosic hydrolyzate. | yeast single-cell protein and yeast extract, in particular, are two products which have many feed, food, pharmaceutical, and biotechnological applications. however, many of these applications are limited by their market price. specifically, the yeast extract requirements for culture media are one of the major technical hurdles to be overcome for the development of low-cost fermentation routes for several top value chemicals in a biorefinery framework. a potential biotechnical solution is the pro ... | 2008 | 18418745 |
| the yeast kluyveromyces marxianus and its biotechnological potential. | strains belonging to the yeast species kluyveromyces marxianus have been isolated from a great variety of habitats, which results in a high metabolic diversity and a substantial degree of intraspecific polymorphism. as a consequence, several different biotechnological applications have been investigated with this yeast: production of enzymes (beta-galactosidase, beta-glucosidase, inulinase, and polygalacturonases, among others), of single-cell protein, of aroma compounds, and of ethanol (includi ... | 2008 | 18427804 |
| simultaneous saccharification and fermentation of kanlow switchgrass pretreated by hydrothermolysis using kluyveromyces marxianus imb4. | a thermotolerant yeast strain named kluyveromyces marxianus imb4 was used in a simultaneous saccharification and fermentation (ssf) process using kanlow switchgrass as a feedstock. switchgrass was pretreated using hydrothermolysis at 200 degrees c for 10 min. after pretreatment, insoluble solids were separated from the liquid prehydrolyzate by filtration and washed with deionized water to remove soluble sugars and inhibitors. insoluble solids were then hydrolyzed using a commercial cellulase pre ... | 2008 | 18546418 |
| identification of yeast and bacteria involved in the mezcal fermentation of agave salmiana. | aims: to identify the yeast and bacteria present in the mezcal fermentation from agave salmiana. methods and results: the restriction and sequence analysis of the amplified region, between 18s and 28s rdna and 16s rdna genes, were used for the identification of yeast and bacteria, respectively. eleven different micro-organisms were identified in the mezcal fermentation. three of them were the following yeast: clavispora lusitaniae, pichia fermentans and kluyveromyces marxianus. the bacteria foun ... | 2008 | 18489025 |
| extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by kluyveromyces marxianus nrrl y-7571. | production of inulinase by solid state fermentation always involves an extraction step, which dictates enzyme recovery yield and is related to cultivation conditions and control of process parameters. this work is focused on the study of extraction conditions aiming to maximize yield of an inulinase obtained by solid state fermentation of sugar cane bagasse and kluyveromyces marxianus nrrl y-7571. kinetics of extraction was followed varying the kind of solvent used. after determining the best so ... | 2008 | 18500581 |
| ethanol production from henequen (agave fourcroydes lem.) juice and molasses by a mixture of two yeasts. | in the fermentation process of henequen (agave fourcroydes lem.) leaf juice, complemented with industrial molasses, the use of an inoculum comprising two yeasts: kluyveromyces marxianus (isolated from the henequen plant) and saccharomyces cerevisiae (commercial strain) was studied. an ethanol production of 5.22+/-1.087% v/v was obtained. contrary to expected, a decrease on ethanol production was observed with the use of the k. marxianus strain. the best results were obtained when a mixture of 25 ... | 2008 | 18524573 |
| lactic acid production by mixed cultures of kluyveromyces marxianus, lactobacillus delbrueckii ssp. bulgaricus and lactobacillus helveticus. | lactic acid production using kluyveromyces marxianus (ifo 288), lactobacillus delbrueckii ssp. bulgaricus (atcc 11842) and lactobacillus helveticus (atcc 15009) individually or as mixed culture on cheese whey in stirred or static fermentation conditions was evaluated. lactic acid production, residual sugar and cell biomass were the main features examined. increased lactic acid production was observed, when mixed cultures were used in comparison to individual ones. the highest lactic acid concent ... | 2008 | 18155517 |
| determination of killer activity in yeasts isolated from the elaboration of seasoned green table olives. | in this work 51 yeasts strains isolated from seasoned green table olives and belonging to the candida, debaryomyces, kluyveromyces, pichia, and saccharomyces genera were characterized by their killer activity in different conditions. killer activity of isolates was analyzed in a medium with different ph's (3.5 to 8.5) and nacl concentrations (5, 8, and 10%). at every ph tested, all the genera studied had killer strains, although the smallest percentages of killer yeasts were found at the highest ... | 2008 | 18077043 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| population diversity of yeasts and lactic acid bacteria in pig feed fermented with whey, wet wheat distillers' grains, or water at different temperatures. | the diversity of populations of yeast and lactic acid bacteria (lab) in pig feeds fermented at 10, 15, or 20 degrees c was characterized by rrna gene sequencing of isolates. the feeds consisted of a cereal grain mix blended with wet wheat distillers' grains (wwdg feed), whey (w feed), or tap water (wat feed). fermentation proceeded for 5 days without disturbance, followed by 14 days of daily simulated feed outtakes, in which 80% of the contents were replaced with fresh feed mixtures. in wwdg fee ... | 2008 | 18223110 |
| production of extracellular exoinulinase from kluyveromyces marxianus ys-1 using root tubers of asparagus officinalis. | root tubers of asparagus officinalis were used as a source of raw inulin for the production of exoinulinase (ec 3.2.1.7) from kluyveromyces marxianus ys-1. root extract prepared at 10kg/cm2 pressure for 10min showed maximum inulinase production. medium components and process parameters were standardized to improve the enzyme production. inulinase yield of 40.2iu/ml in a medium containing raw inulin (3.5%), beef extract (2%), sds (0.001%), mn2+ (2.0mm), mg2+ (1.5mm), co2+ (2mm) and ph 6.5 has bee ... | 2008 | 18280145 |
| appropriate sampling for intracellular amino acid analysis in five phylogenetically different yeasts. | methanol quenching and fast filtration, the two most common sampling protocols in microbial metabolome analysis, were validated for intracellular amino acid analysis in phylogenetically different yeast strains comprising saccharomyces cerevisiae, kluyveromyces marxianus, pichia pastoris, schizosaccharomyces pombe and zygosaccharomyces bailii. with only few exceptions for selected amino acids, all yeasts exhibited negligible metabolite leakage during quenching with 60% cold buffered methanol. sli ... | 2008 | 18604477 |
| [one-step ethanol fermentation with kluyveromyces marxianus yx01 from jerusalem artichoke]. | a unique one-step ethanol fermentation process was developed with the inulinase-producing strain kluyveromyces marxianus yx01. firstly, the impact of temperature on ethanol fermentation was investigated through flask fermentation, and the temperature of 35 degrees c was observed to be the optimum to coordinate inulinase production, inulin saccharification and ethanol fermentation. and then, the impact of aeration and substrate concentration was studied through batch fermentation in the 2.5 l fer ... | 2008 | 19256341 |
| identification of yeasts and evaluation of their distribution in taiwanese kefir and viili starters. | the objective of the present study was to investigate yeast communities in kefir grains and viili starters in taiwan through conventional microbiological cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). the dna sequencing was used as a validity technique to ensure that all isolates within each group belonged to just one species, and to confirm the identified results of pcr-dgge. results indicated that a combination of conventional microbiological cult ... | 2008 | 18832201 |
| high-temperature ethanol fermentation and transformation with linear dna in the thermotolerant yeast kluyveromyces marxianus dmku3-1042. | we demonstrate herein the ability of kluyveromyces marxianus to be an efficient ethanol producer and host for expressing heterologous proteins as an alternative to saccharomyces cerevisiae. growth and ethanol production by strains of k. marxianus and s. cerevisiae were compared under the same conditions. k. marxianus dmku3-1042 was found to be the most suitable strain for high-temperature growth and ethanol production at 45 degrees c. this strain, but not s. cerevisiae, utilized cellobiose, xylo ... | 2008 | 18931291 |
| comparison of enzymatic antioxidant defence systems in different metabolic types of yeasts. | the enzymatic defence system in the 2 yeasts kluyveromyces marxianus and rhodotorula glutinis, differing in their mode of oxygen uptake and energy generation, was characterized and compared with the well-studied facultatively fermentative crabtree-positive saccharomyces cerevisiae strain. twofold higher superoxide dismutase (sod) and catalase activities were detected in k. marxianus and r. glutinis when cells were cultured on glucose. further increases of 10%-15% in sod activity and 30%-50% in c ... | 2008 | 18997852 |
| bioconversion of brewer's spent grains to bioethanol. | spent grains (sg), the residue remaining after extraction of wort, are a major by-product of brewing. this lignocelluose-rich biomass may provide a source of sugars for fuel ethanol fermentations. dilute acid and enzyme treatments were developed to convert the hemicellulose and cellulose fractions to glucose, xylose and arabinose. pretreatment of dried, milled grains with 0.16 n hno(3) at 121 degrees c for 15 min was chosen as the most suitable method for solubilizing grains before enzymatic dig ... | 2008 | 18547331 |
| effect of the yeast and bacteria biomass on the microbiota in the rumen. | this study aims at obtaining a probiotic product based on viable biomass from 6 yeast strains and 2 strains of lactic bacteria used for nutrition of animals. the strains are subjected to some resistance tests, at temperature, ph, pepsin, pancreatin and biliary salts so as to make obvious their viability. tests were done by comparison to the witness strain and respectively a protective solution based on mucin and casein. based on the resulted viabilities 2 products are formulated. their effect is ... | 2008 | 19137830 |
| ethanol fermentation with kluyveromyces marxianus from jerusalem artichoke grown in salina and irrigated with a mixture of seawater and freshwater. | to study fuel ethanol fermentation with kluyveromyces marxianus atcc8554 from jerusalem artichoke (helianthus tuberosus) grown in salina and irrigated with a mixture of seawater and freshwater. | 2008 | 19120653 |
| proteinase and phospholipase activity as virulence factors in candida species isolated from blood. | the number of nosocomial blood stream infections due to candida species has increased over the past few decades. in order to establish an infection, opportunistic pathogens have to evade the immune system, survive, divide in the host environment, and spread to other tissues. proteinase and phospholipase secretion has been implicated as potential virulence factors for some candida species responsible for catheter related candidemia in intensive care unit (icu) patients with indwelling devices. we ... | 2008 | 19071887 |
| a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius. | samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ... | 2008 | 18774196 |
| fermentation of cacao (theobroma cacao l.) seeds with a hybrid kluyveromyces marxianus strain improved product quality attributes. | fermentation of theobroma cacao (cacao) seeds is an absolute requirement for the full development of chocolate flavor precursors. an adequate aeration of the fermenting cacao seed mass is a fundamental prerequisite for a satisfactory fermentation. here, we evaluated whether a controlled inoculation of cacao seed fermentation using a kluyveromyces marxianus hybrid yeast strain, with an increased pectinolytic activity, would improve an earlier liquid drainage ('sweatings') from the fermentation ma ... | 2008 | 18616604 |
| lactase production by solid-state cultivation of kluyveromyces marxianus cdbbl 278 on an inert support: effect of inoculum, buffer, and nitrogen source. | a study was carried out to select the conditions for cultivation of kluyveromyces marxianus cdbbl 278 in solid-state culture (ssc) using polyurethane foam (puf) as an inert support. puf was impregnated with culture media containing lactose (50 g/l) as the carbon and energy source. evaluation of culture parameters during different growth phases was carried out by respirometry. the effect of inoculum level, buffer capacity of the medium, and nitrogen source upon the yield of biomass on lactose (yx ... | 2008 | 18592410 |
| production of β-galactosidase by kluyveromyces marxianus mtcc 1388 using whey and effect of four different methods of enzyme extraction on β-galactosidase activity. | whey containing 4.4% (w/v) lactose was inoculated with kluyveromyces marxianus mtcc 1389 for carrying out studies related to β-galactosidase production. β-galactosidase activity was found to be maximum after 30 h and further incubation resulted in decline in activity. the maximum cell biomass of 2.54 mg ml(-1) was observed after 36 h of incubation. lactose concentration dropped drastically to 0.04 % from 4.40% after 36 h of incubation. out of the four methods tested for extraction of enzyme, sds ... | 2008 | 23100731 |
| over-expression of daao and catalase in kluyveromyces marxianus through media optimization, permeabilization and ga stabilization techniques. | the selected thermotolerant, lactose-utilizing yeast strain kluyveromyces marxianus nbimcc 8362 possesses high specific d-amino acid oxidase activity (60ug(-1)), which was increased nine-fold (545ug(-1)) by design of the growth medium and conditions for d-amino oxidase induction. applying an optimized simple and rapid procedure for chemical permeabilization of k. marxianus cells with the cationic detergent cetyltrimethylammonium bromide, the enzyme activities (d-amino acid oxidase and catalase) ... | 2008 | 22578860 |
| purification, crystallization and preliminary x-ray analysis of beta-glucosidase from kluyveromyces marxianus nbrc1777. | the intracellular beta-glucosidase from kluyveromyces marxianus nbrc1777 (kmbgli) belongs to glycoside hydrolase family 3 and has a unique domain architecture. selenomethionine-labelled kmbgli was purified and crystallized by the hanging-drop vapour-diffusion method using the purified enzyme at 30 mg ml(-1), 0.04 m potassium dihydrogen phosphate ph 5.1, 16%(w/v) peg 8000 and 20%(v/v) glycerol. the crystal belonged to space group c2, with unitcell parameters a = 245.8, b = 148.7, c = 119.9 angstr ... | 2009 | 19923748 |
| the crucial role of alcohol dehydrogenase adh3 in kluyveromyces marxianus mitochondrial metabolism. | the function of mitochondrial adh3 in the thermotolerant yeast kluyveromyces marxianus was investigated. an adh3-disrupted mutant exhibited growth retardation on non-fermentable carbon sources, except for ethanol, and this was suppressed by supplementation with antioxidants. detailed analysis of the phenotype revealed that the mutant showed an increase in the activity of nadh dehydrogenase, sensitivity to h(2)o(2), and accumulation of reactive oxygen species (ros), and that these carbon sources ... | 2009 | 19966463 |
| cloning, characterization, and functional analysis of the epg1-2 gene: a new allele coding for an endopolygalacturonase in kluyveromyces marxianus. | a new allele, the epg1-2 gene, which codes for an endopolygalacturonase in kluyveromyces marxianus cect1043, has been cloned. the gene has 1086 bp and the protein 362 amino acids, one more than the previously described epg1p. epg1-2p shows a high degree of similarity with the polygalacturonases of fungi and yeasts. the sequences common to all of the polygalacturonases of prokaryotes, fungi, and higher plants are also conserved in epg1-2p. the epg1-2 gene has been expressed in pichia pastoris , a ... | 2009 | 19725536 |
| [characterization of a chitosanase from fusarium solani and its expression in an industrial strain of saccharomyces cerevisiae]. | to investigate the biochemical characteristics of the chitosanase from fusarium solani, and its application in chitooligosaccharides production, and express the chitosanase gene (csn) in a saccharomyces cerevisiae industrial strain. | 2009 | 20222446 |
| [oligomeric structure and physicochemical properties of inulinase from kluyveromyces marxianus y-303]. | it has been found using a combination of atomic force microscopy with infrared spectroscopy, gel chromatography, and electrophoresis that inulinase from kluyveromyces marxianus y-303 has oligomeric structure, which includes two subunits differing in size, molecular mass, and catalytic activity. it has been shown that the division of the inulinase dimer into monomers leads to an increase in the number of irregular sites by 6% for subunit 1 (54.8 kda) and by 10% for subunit 2 (8.4 kda) compared wi ... | 2009 | 20067178 |
| the microbiota of lafun, an african traditional cassava food product. | lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ... | 2009 | 19493582 |
| main microorganisms involved in the fermentation of ugandan ghee. | ghee is widely produced from a traditional fermented butter-like product named mashita in western uganda. however, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. the aim of this study was to identify the microorganisms present at the end of mashita ripening using culture-dependent and culture-independent techniques. the most commonly identified species of lactic acid bacteria (lab) in mashita using culture-dependent techniques were lactobacill ... | 2009 | 19577815 |
| evaluation of chrom-pal medium for the isolation and direct identification of candida dubliniensis in primary cultures from the oral cavity. | candida albicans is the species most frequently isolated from oral specimens, but the recovery of other candida species such as candida dubliniensis is increasing. differentiation of c. dubliniensis from c. albicans requires special tests and both species are misidentified in some studies. chrom-pal (ch-p) is a novel chromogenic medium used in our laboratory for differentiation between c. albicans and c. dubliniensis on the basis of colony colour and morphology, and chlamydospore production. the ... | 2009 | 19608690 |
| effect of phenotypic switching on expression of virulence factors by candida albicans causing candidiasis in diabetic patients. | a total of 110 strains belonging to seven species of candida were isolated from various forms of candidiasis in diabetic patients. they were candida albicans 53 (47%), candida tropicalis 36 (33%), candida glabrata 9 (8%), candida parapsilosis 4 (4%), candida guilliermondii 2 (2%), candida krusei 5 (5%) and candida kefyr 1 (1%). all 53 strains of c. albicans isolated were observed to express virulence factors such as cell surface hydrophobicity (csh), adherence to human buccal epithelial cell (be ... | 2009 | 19635443 |
| enhancement of survival of probiotic and non-probiotic lactic acid bacteria by yeasts in fermented milk under non-refrigerated conditions. | the effects of yeasts on the survival of probiotic and non-probiotic lactic acid bacteria (lab) were studied in fermented milk under non-refrigerated conditions (30 degrees c) with a view to develop ambient-stable fermented milk with live lab. five yeasts tested (saccharomyces bayanus, williopsis saturnus var. saturnus, yarrowia lipolytica, candida kefyr and kluyveromyces marxianus) enhanced the survival of lactobacillus bulgaricus (but not streptococcus thermophilus) in a mixed yoghurt culture ... | 2009 | 19666198 |
| microbiological and chemical properties of kefir manufactured by entrapped microorganisms isolated from kefir grains. | in this study, various yeasts (kluyveromyces marxianus, saccharomyces turicensis, pichia fermentans) and lactic acid bacteria (lactobacillus kefiranofaciens, lactobacillus kefiri, leuconostoc mesenteroides) were entrapped in 2 different microspheres using an entrapment ratio for the strains that was based on the distribution ratio of these organisms in kefir grains. the purpose of this study was to develop a new technique to produce kefir using immobilized starter cultures isolated from kefir gr ... | 2009 | 19528577 |
| synthesis, characterization and antimicrobial activity of water soluble dendritic macromolecules. | several families of water soluble dendrimers were synthesized based on poly(propyleneoxide) amines (jeffamines) (p(1)). p(1)-core and branched units were constructed from both methylacrylate and ethylenediamine (p(2)-p(9), and generations 0-3 with -nh(2), -cooh functionalities). they were characterized by elemental analysis (ea), gel permeation chromatography (gpc), ft-ir, (1)h, and (13)c nmr. the antimicrobial activities of only water soluble compounds (p(1), p(3), p(4), p(6), p(7) and p(9)) we ... | 2009 | 18657884 |
| purification and partial characterization of cu/zn superoxide dismutase from kluyveromyces marxianus yeast. | a new thermostable cu/zn sod from a thermotolerant yeast strain kluyveromyces marxianus nbimcc 1984 has been purified and characterized. the purification procedure comprises thermal treatment and dialysis, ion-exchange chromatography and chromatofocusing. the methodology is a rapid, efficient and highly specific, generating pure preparation (specific activity 996 u mg of protein(-1)) with a yield of 53%. the purified enzyme is a homodimer with mw of 34,034 da and has high n-terminal homology wit ... | 2009 | 19758847 |
| effect of different cellulase dosages on cell viability and ethanol production by kluyveromyces marxianus in ssf processes. | this study was aimed to study the effect of commercial cellulases (celluclast 1.5 lfg) on kluyveromyces marxianus cect 10875 growth and ethanol production in ssf processes. preliminary tests carried out in glucose (50 g/l) fermentation medium showed that high enzyme amounts (2.5-3.5 fpu/ml) could cause a negative effect on k. marxianus growth rate and viable cells number. however, the maximum ethanol production was not affected and about 86% of the theoretical (22 g/l) was reached in all cases i ... | 2009 | 18722114 |
| cloning and characterization of the inulinase gene from a marine yeast pichia guilliermondii and its expression in pichia pastoris. | the extracellular inulinase structural gene was isolated from the genomic dna of the marine yeast pichia guilliermondii strain 1 by pcr. the gene had an open reading frame of 1,542 bp long encoding an inulinase. the coding region of the gene was not interrupted by any intron. it encoded 514 amino acid residues of a protein with a putative signal peptide of 18 amino acids and the calculated molecular mass of 58.04 kda. the protein sequence deduced from the inulinase structural gene contained the ... | 2009 | 18821076 |
| analysis of the microflora in tibetan kefir grains using denaturing gradient gel electrophoresis. | the microflora of tibetan kefir grains was investigated by culture- independent methods. denaturing gradient gel electrophoresis (dgge) of partially amplified 16s rrna for bacteria and 26s rrna for yeasts, followed by sequencing of the most intense bands, showed that the dominant microorganisms were pseudomonas sp., leuconostoc mesenteroides, lactobacillus helveticus, lactobacillus kefiranofaciens, lactococcus lactis, lactobacillus kefiri, lactobacillus casei, kazachstania unispora, kluyveromyce ... | 2009 | 19835760 |
| molecular identification of yeasts associated with traditional egyptian dairy products. | this study aimed to examine the diversity and ecology of yeasts associated with traditional egyptian dairy products employing molecular techniques in yeast identification. a total of 120 samples of fresh and stored domiati cheese, kariesh cheese, and "matared" cream were collected from local markets and examined. forty yeast isolates were cultured from these samples and identified using the restriction-fragment length polymorphism (rflps) of 5.8s-its rdna region and sequencing of the domains d1 ... | 2009 | 19895478 |
| super life--how and why 'cell selection' leads to the fastest-growing eukaryote. | what is the highest possible replication rate for living organisms? the cellular growth rate is controlled by a variety of processes. therefore, it is unclear which metabolic process or group of processes should be activated to increase growth rate. an organism that is already growing fast may already have optimized through evolution all processes that could be optimized readily, but may be confronted with a more generic limitation. here we introduce a method called 'cell selection' to select fo ... | 2009 | 19087200 |
| [effect of naphthalene and its derivatives on candida pseudotropicalis 44 pk sensitivity to trichothecene mycotoxins]. | availability of t-2 toxin and ht-2 toxin in cereals and grain-crops is determined by a bioauthography or the method of paper disks using yeast candida pseudotropicalis 44 pk. with the purpose of increasing the method sensitivity the culture medium for c.pseudotropicalis 44 pk was enriched by t-2 toxin, naphthalene, 1-naphthol, 2-naphthol, 1-naphthylacetate, 2-naphthylacetate, 1-naphthylamine and 1-nitroso-2-naphthol. the addition of any studied compounds, except for t-2 toxin, resulted in potent ... | 2009 | 19938611 |
| construction of flocculent kluyveromyces marxianus strains suitable for high-temperature ethanol fermentation. | flocculating yeasts are highly useful in fermentation processes because these cells can be separated easily from the fermentation mash. however, native yeasts are usually non-flocculating, including kluyveromyces marxianus, which exhibits a potent high-temperature ethanol fermentation ability. we describe here the construction of flocculent k. marxianus strains via the introduction of the flo1, flo5, flo9, and flo10 genes from saccharomyces cerevisiae. the s. cerevisiae flo genes were overexpres ... | 2009 | 19420680 |