Publications

expression of lectin-binding surface glycoproteins during the development of schistosoma mansoni schistosomula.	tegumental glycoproteins of schistosoma mansoni cercariae, mechanically produced 24-hr and 48-hr schistosomula, and adult worms were radioiodinated with the bolton-hunter reagent, then isolated by lectin affinity chromatography. sds-page revealed con a binding glycoproteins with apparent molecular weights of 180,000, 150,000, 43,000, and 30,000 in detergent extracts of the tegument of cercariae. these glycoproteins are retained by 24-hr mechanically produced, cultured schistosomula and are accom ...	1986	3819968
determination of attachment sites for n-linked carbohydrate groups of class ii histocompatibility alpha-chain and analysis of possible o-linked glycosylation of alpha- and gamma-chains.	two glycopeptide fractions were isolated from a tryptic digest of the human class ii antigen alpha-subunit, by chromatography on lens culinaris and ricinus communis lectin columns, respectively. partial nh2-terminal sequence analysis of radiochemically labelled glycopeptide fractions allowed alignment with two stretches of the deduced dr alpha sequence, each encompassing a signal for n-linked glycosylation, i.e. asn-x-thr(ser). the fraction displaying affinity for l. culinaris lectin was suscept ...	1986	3457263
characterization of surface glycoproteins on schistosoma mansoni adult worms by lectin affinity chromatography.	adult schistosoma mansoni were radiolabeled by direct radioiodination using the bolton-hunter reagent or by metabolic labeling using radioactive hexose precursors. tegumental material was extracted by freeze-thaw or by incubation in the non-ionic detergent nonidet p-40, then applied to chromatography columns containing the following immobilized lectins: con a, lentil lectin, wheat germ agglutinin, soybean agglutinin and the agglutinins from ricinus communis and helix pomatia. sds-page analysis o ...	1986	3755470
alteration by concanavalin a of the slow dissociable component in the human growth hormone-receptor interaction.	in mice liver plasma membranes (pm), the binding affinity of receptors for [125i] human growth hormone (hgh) was dependent on the association time: after 18 hours, a high affinity receptor form with ka = 6.8 x 10(9) m-1 accumulated and, as compared to after 1 hour, an increase up to 88%, in a slow dissociating component was observed. preincubation of pm with concanavalin a (con a) or other lectins from lens culinaris (lca), ricinus communis (rca i), wheat germ agglutinin (wga) specifically inhib ...	1986	3758923
structural analysis of the oligosaccharides of dr1 and dqw1 molecules.	the major glycopeptide fractions of the alpha- and beta-chains of hla-dr1 and dqw1 molecules were isolated on columns of immobilized concanavalin a (con a), lens culinaris (lens), ricinus communis agglutinin type i (rca), and leuko-phytohemagglutinin. oligosaccharides were prepared from these fractions by enzymatic digestion with endoglycosidases h or f and were analyzed on bio-gel p-6. the glycopeptides tightly bound to con a (cona iii) were mostly associated with alpha-chains and were resolved ...	1986	3486906
purification and characterization of the extracellular acid phosphatase of leishmania donovani.	a tartrate sensitive acid phosphatase activity was purified from culture supernatants of leishmania donovani promastigotes grown in a macromolecule-free defined medium. purification was accomplished by ultrafiltration, lentil lectin affinity chromatography, and sucrose density gradient ultracentrifugation. this enzyme was determined to be an acid glycoprotein containing 0.37 mg hexose per mg protein. a molecular weight of 134 000 by sucrose density gradient centrifugation was observed, although ...	1986	3762593
purification of human macrophage colony stimulating factor (csf-1) from medium conditioned by pancreatic carcinoma cells.	colony stimulating factor-1 has been purified to apparent homogeneity from the serum-free medium conditioned by cultured human pancreatic carcinoma cells which had been induced with phorbol myristate acetate. the purification scheme consisted of sequential steps of batchwise adsorption to calcium phosphate gel, adsorption to lentil lectin-sepharose, binding to immobilized antibodies, hydrophobic interaction chromatography, and reversed-phase high-performance liquid chromatography. the purified g ...	1986	3488735
characterization of human t cell-derived ige-potentiating factor.	we have previously shown that fc epsilon receptor-positive (fc epsilon r+) t cell lines from patients with the hyper ige syndrome secrete ige-binding factors which selectively enhance ige but not igg synthesis in cultures of b cells obtained from patients with allergic rhinitis but not from nonatopic subject. in the present study we have tested the effect of supernatants from fc epsilon r+ t cell lines on a large panel of b cells from atopic patients (n = 20). we found that ige synthesis was sel ...	1986	3488913
monoclonal antibody affinity purification of a leishmania membrane glycoprotein and its inhibition of leishmania-macrophage binding.	specific monoclonal antibody coupled to affi-gel 10 was used to purify a major membrane glycoprotein of leishmania mexicana amazonensis, one of a group of parasitic protozoa that specifically infect mammalian macrophages. immobilized antigen was eluted at a 34% efficiency with buffers at either ph 2.5 or 11 or with mgcl2, but only the antigen eluted under basic conditions could be readsorbed to the immunobeads. sephacryl s-300 gel filtration of the purified antigen gave a single peak of protein ...	1986	3079902
inhibition of mitogenesis induced by phytohemagglutinin and lens culinaris lectin in adherent-cell supernatants treated with protein extract of mycobacterium tuberculosis.	specific stimulation of t cells by phytohemagglutinin and lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (cfpe) derived from bacterial cultures of mycobacterium tuberculosis h37ra (avirulent) and h37rv (virulent). the induction of the inhibitory factor was blocked by the presence of hyperimmune antisera to h37rv or h37ra cfpe. the inhibitory factor did not seem to be a cfpe reprocessed by the adherent ...	1986	3082760
membrane glycophorins in sta blood group erythrocytes.	structural and immunochemical studies of glycophorins isolated from erythrocytes of an individual homozygous for the m sta blood group phenotype are described. reactivities with specific monoclonal antibodies indicated that two major m and n glycophorins were present. the m and n sta glycophorins were resolved by lens culinaris lectin affinity chromatography. the n species was not held on the lectin but the m species, like control alpha glycophorins, was retained and could be eluted with alpha-m ...	1986	3514617
murine iga binding factors (iga-bf) suppressing iga production: characterization and target specificity of iga-bf.	chemical and functional properties of iga binding factor(s) (iga-bf) from both murine con a-activated spleen cells and fc gamma r+, fc alpha r+ t hybridoma cells (t2d4) were studied. iga-bf produced from the cells after preculture with iga were purified with iga-sepharose. purified iga-bf inhibited the binding of iga to fc alpha r+ l5178y t lymphoma cells, and class-specifically suppressed in vitro iga synthesis of the pokeweed mitogen (pwm)-stimulated murine spleen cells. both iga-specific supp ...	1986	3514749
purification and characterization of a neutrophil chemotactic factor from dirofilaria immitis.	a neutrophil chemotactic factor (ncf-di) was purified from a crude extract of dirofilaria immitis adult worm by a combination of anion-exchange chromatography on de52 and gel filtration on sephacryl s-200. ncf-di showed a single protein band by both polyacrylamide gel electrophoresis (page) and sodium dodecyl sulfate (sds) page. the molecular weight of ncf-di was estimated to be 17,000 by gel filtration on sephadex g-150, and 14,000 by sds-page. ncf-di was an acidic protein with isoelectric poin ...	1986	3525795
[purification and properties of bordetella pertussis toxin].	the modified method for the isolation and purification of b. pertussis toxin has been proposed. chromatography with the use of hydroxylapatite and lentil lectin--sepharose 4b has permitted the isolation of the preparation purified 600 times. its molecular weight is about 90,000. the preparation has been found to possess leukocytosis-stimulating, histamine-sensitising and hemagglutinating activity. electrophoretic analysis has revealed that the isolated substance consists of four subunits with mo ...	1986	3529763
effects of different proportions of carbohydrates, polysaccharides/monosaccharides, and different fibers on the metabolic control in diabetic rats.	recent findings have suggested that diets with a high level of carbohydrates may impair the metabolic control of diabetes mellitus in humans. moreover, other investigations have indicated that if the simple sugar content is increased in order to attain a proportion of polysaccharides/monosaccharides equal to 1, then neither the blood glucose nor the lipidic response show any change. we have studied the effect of increasing carbohydrates in the diet (59% v 82%), while maintaining cereal fiber lev ...	1986	3020344
affinity to lectin, biological and immunological characteristics of human chorionic gonadotropins from pregnant women and trophoblastic tumour patients.	to compare the affinity to lectin of human chorionic gonadotropins (hcg) from pregnant women and trophoblastic tumour patients, a small amount of urine was fractionated by a lentil lectin (lch) affinity chromatography. the lch-bound fractions were eluted with 2% mannoside solution, and each fraction was assayed for hcg activities by radioimmunoassay. in pregnant women, more than 90% of hcg immunoactivity in urine was bound to the lch column and eluted from it, whereas 8 to 26% and 37 to 51% of t ...	1986	3019056
regulatory effects of human ige-binding factors on the ige response of rat lymphocytes.	normal human peripheral blood t cells were propagated in the presence of human interleukin 2, and activated cells were incubated with human ige-dimer to induce ige binding factor formation. the cells were then fused with a mutant of the human t cell line cem. five of the t cell hybridomas formed ige binding factors upon incubation with human ige-dimer. because ige binding factors formed by the human t cell hybridomas had affinity not only for human ige but also for rat ige, the biologic activiti ...	1986	3079805
chemical induction of sister-chromatid exchanges in human lymphocytes treated in g0 prior to stimulation by different mitogens and revealed 72 h later in second division cells.	frequencies of sister-chromatid exchanges (sce) were determined in second-division metaphases of human lymphocytes, exposed for 1 h during the g0 phase to mitomycin c (mmc) alone or to cyclophosphamide (cp) in the presence of s9 mix. the cells were then cultured for 72 h in the presence of phytohemagglutinin (pha), concanavalin a (con a), wistaria floribunda (wfa) or lens culinaris (lch-a) extracts. large differences in mitotic indices (mi) and cell-cycle kinetics were observed among cells subje ...	1986	3084967
characterization of the fertilization antigen 1 for the development of a contraceptive vaccine.	a fertilization antigen, fa-1, was purified from either deoxycholate- or lithium diiodosalicylate-solubilized murine testes by immunoaffinity chromatography using a monoclonal antibody, ma-24, which inhibited fertilization in vitro. the fa-1 was recovered at high (11.4) or low (2.8) ph using stepwise elution procedures of the deoxycholate or lithium diiodosalicylate extracts, respectively. both of these fractions showed a single band of 47 kda when analyzed by nadodso4/page and silver staining. ...	1986	3461457
lectin-binding sites in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa.	the carbohydrate structures of cellular glycoconjugates in normal, hyperplastic, adenomatous and carcinomatous human colorectal mucosa were analysed with six fluorescein isothiocyanate-conjugated lectins. in normal, hyperplastic and adenomatous colorectal mucosa showing mild or moderate dysplasia concanavalin a (con a), lens culinaris (lca), and wheat germ (wga) agglutinins stained goblet cell glycoconjugates (actual mucin goblet itself) while peanut (pna), vicia villosa (vva), and griffonia sim ...	1986	3092568
characterization of lectin-binding lymphocytes in goats with caseous lymphadenitis.	numbers of lymphoid cells bearing markers for peanut agglutinin, wheat germ agglutinin, and anti-immunoglobulin g in healthy goats and in goats with caseous lymphadenitis were determined by use of immunofluorescent techniques. results indicated that, when compared with healthy goats, diseased goats had normal numbers of anti-immunoglobulin g-positive cells (b cells), had significantly reduced numbers of peanut agglutinin-positive cells (t cells), and had fewer cells with receptor sites for lens ...	1986	3487998
the human growth hormone receptor of cultured human lymphocytes. structural characteristics and glycosylation properties.	the structural characteristics and glycoprotein nature of the human growth hormone (hgh) receptor in cultured lymphocytes (im-9 cell line) were studied with the use of a bifunctional reagent (disuccinimidyl suberate) to couple 125i-hgh covalently to intact cells. after cross-linking, the hormone-receptor complexes were analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. a single band of mr 140,000 was identified under reducing conditions. the labelling of this band was blocke ...	1986	3099769
binding of a synthetic analogue of mitogenic bacterial lipoprotein to murine major histocompatibility complex (mhc) gene products.	lipoprotein from the outer membrane of escherichia coli and a synthetic analogue of its n-terminal lipopeptide part, tripalmitoyl-pentapeptide, constitute potent mitogens and polyclonal activators of murine b-lymphocytes in vitro. when entering the circulation after intravenous administration in experimental animals, they interact with the humoral and cellular elements of the blood, which results in splenomegaly and b-lymphocyte activation in vivo. we investigated lipopeptide-binding proteins in ...	1986	3491614
comparisons between true digestibility of total nitrogen and limiting amino acids in vegetable proteins fed to rats.	values (%) for true digestibility (td) of protein and individual amino acids in some vegetable proteins were determined by the rat balance (fecal) method. diets containing 8% crude protein (n x 6.25) from soaked and autoclaved samples of trapper and century field peas, lentil, pinto bean, seafarer bean, black bean or fababean and autoclaved samples of soybean, peanut, sunflower, rolled oat, rice + soybean and corn + pea were tested in two rat balance studies. in the case of blends, each protein ...	1986	3746456
purification and chemical composition of gpl115, the human lymphocyte surface sialoglycoprotein that is defective in wiskott-aldrich syndrome.	gpl115, the surface sialoglycoprotein that is defective in lymphocytes of wiskott-aldrich syndrome patients has been purified from large scale cultures of the lymphoblastoid line cem. the purification entails cell lysis and solubilization of gpl115 with the detergent nonidet p-40, sequential affinity chromatography on lentil lectin-sepharose, wheat germ lectin-sepharose, and, after treatment with sialidase, on peanut lectin-sepharose. sepharose cl-6b gel filtration removes residual protein conta ...	1986	3711098
energy utilization of supplemented cereal diets in human volunteers.	energy utilization was studied in human volunteers using different diets containing wheat flour supplemented by groundnut (arachis hypogaea), "masur" (lens culinaris), mung (phaseolus aureus) and gram (cicer arietinum) flour. digestible and metabolizable energies were determined for all the experimental diets. an improved energy digestibility was observed when wheat flour was supplemented with groundnut flour, and groundnut flour plus gram flour, i.e. 93.35 and 89.48%, respectively. percent dige ...	1986	3632213
interaction of rat liver glucocorticoid receptor with lectins: is the glucocorticoid receptor a glycoprotein?	although glucocorticoid receptors have been extensively studied in a variety of tissues, the precise nature of the receptor protein still remains unknown. to further characterize this protein we assessed the effects of various lectins on [3h]dexamethasone binding to prepurified preparations of rat liver glucocorticoid receptor. among the lectins tested only ulex europeus and lens culinaris induced a concentration-related decrease in [3h]dexamethasone binding. following ulex europeus or lens culi ...	1986	3702409
similarities in glycosylation of human neuroblastoma tumors and cell lines.	the presence of fucosyl residues linked alpha 1----3(4) to n-acetylglucosamine was demonstrated on the oligosaccharides from glycoproteins of 11 human neuroblastoma tumors from ten different patients. this finding is in complete agreement with the previous report that human neuroblastoma cell lines contained an unusually large proportion of metabolically incorporated l-[3h]fucose in this specific linkage (u. v. santer and m. c. glick, cancer res., 43:4159-4166, 1983). furthermore, the glycopepti ...	1986	3708596
hepatocyte adhesion to collagen. isolation of membrane glycoproteins involved in adhesion to collagen.	adhesion of hepatocytes to collagenous substrates and their spreading have been shown to involve a specific recognition event, possibly mediated by membrane proteins with affinity for collagen. in the present communication, we describe the isolation of membrane components that are involved in the adhesion of rat hepatocytes to collagen. these components could be solubilized from liver microsomal membranes by treatment with detergents or papain--but not by treatment with edta, urea or high salt. ...	1986	3956590
biochemical investigation of the pathogenesis of heymann nephritis.	this study describes biochemical comparison of proximal tubule antigens from the brush border membrane (bbm) of dog and rat kidney. the purpose was to determine if a difference in bbm composition could explain the inability to produce either active or passive heymann nephritis in the dog. although the membrane composition as revealed by coomassie blue staining on 4 to 11 per cent polyacrylamide electrophoresis varied considerably between rats and dogs, polyclonal antibodies (rabbit anti-rat, rab ...	1986	3747348
affinity chromatography and sds-page studies of radiolabeled ige-binding and igg-binding factors generated from human lymphoblastoid cell lines.	soluble ige-binding and igg-binding factors were generated by 18-hour incubation at 4 degrees c of the human b cell lines rpmi 8866 and daudi. these cells express fc receptors for ige (fc epsilon r) and igg (fc gamma r), respectively. binding factors specifically inhibited fcr on both lymphocytes and monocytes, and bound to ig-sepharose supports. rpmi 8866 cells and daudi cells were radiolabeled with 125i by the lactoperoxidase method, and the soluble factors were labeled by the chloramine t met ...	1986	3759254
oligodendrocyte cell adhesion molecules are related to neural cell adhesion molecule (n-cam).	the glycoproteins responsible for calcium-dependent oligodendrocyte aggregation were purified and characterized. using detergent extraction, lentil-lectin-sepharose 4b affinity chromatography, and preparative gel electrophoresis, 3 proteins were purified to apparent homogeneity, with relative mrs of 120,000, 140,000, and 180,000. the aggregation assay showed that all 3 proteins had the ability to block antibody-mediated inhibition of oligodendrocyte aggregation. the 120,000 protein was the most ...	1986	3772436
analysis of normal human tonsil class ii antigen glycosylation by lectin affinity chromatography.	human class ii molecules include the hla-dr, -dq, and -dp alloantigens. each class ii molecule consists of two glycosylated polypeptide chains, the alpha chain and the beta chain. we have used lectin affinity analysis to investigate the glycosylation pattern of [3h]mannose-labeled glycopeptides derived from isolated alpha and beta chains of hla-dr and -dq molecules of normal tonsil cells. glycopeptides obtained by pronase digestion of each isolated chain were passed sequentially over columns of ...	1986	3782152
characterization of the carbohydrate moiety of clerodendron trichotomum lectins. its structure and reactivity toward plant lectins.	lectins were isolated from fruits and leaves of clerodendron trichotomum by affinity chromatography on lactamyl-sepharose. the purified lectins (c. trichotomum agglutinin: cta) were homogeneous on sds/polyacrylamide gel electrophoresis, and the carbohydrate moiety was characterized by physicochemical and immunochemical methods. the asparagine-linked oligosaccharides were released by treatment with n-oligosaccharide glycopeptidase (almond, ec 3.5.1.52) of peptic glycopeptides obtained from fruit ...	1986	3792315
purification and chemical characterization of human platelet membrane glycoprotein iv.	human platelet membrane glycoprotein iv (gpiv), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of lens culinaris agglutinin-sepharose and wheat germ agglutinin-sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sds). on sds-polyacrylamide gel electrophoresis in either the presence or absence of dithiothreitol, gpiv gave a single band with an apparent molecular weight of 97,000, ...	1986	3818560
assessment of lymphocyte function during vitamin a deficiency.	two variables of immune status were measured in vitamin a-deficient (a-), vitamin a-sufficient pair-fed (a + pf), and healthy control (a+) rats to examine the mechanisms of immune regulation by vitamin a. parallel samples were run in bovine fetal serum (bfs)-containing medium and serum-free medium to control bfs-origin vitamin a in the cultures. splenocytes derived from a- rats showed significantly depressed blastogenic response to all lectins tested in both mediums when compared with responses ...	1986	3963587
differential redistribution of lectin receptor classes on clonal rat myotubes and myoblasts.	to evaluate the relative mobilities of cell surface glycoconjugates during myogenesis we have studied the redistribution of fluorescein-conjugated plant lectins on l6 rat myogenic cells. previous experiments had demonstrated that the receptors for the lectins soybean agglutinin (sba), wheat germ agglutinin, concanavalin a and lens culinaris agglutinin all were relatively uniformly distributed on both myoblasts and myotubes, and that sba receptors were capable of rapid redistribution on myotubes ...	1986	3805140
the presence of an extracellular matrix between cells involved in the determination of the mesoderm bands in embryos ofpatella vulgata (mollusca, gastropoda).	in 32-cell stage embryos ofpatella vulgata one of the macromeres contacts the animal micromeres, and as a result is induced to differentiate into the stem cell of the mesodermal cell line. in this study we show the presence of an extracellular matrix (ecm) between these two interacting cell types. the ecm appears to be formed by the micromeres during the 32-cell stage. staining experiments with alcian blue and tannic acid indicate that in contains glycoconjugates, possibly in the form of proteog ...	1986	28306013
suppression of low-dose streptozotocin-induced diabetes by immunomodulatory lectins.	the effect of immunomodulatory lectins on diabetes development following low-dose streptozotocin treatment in inbred mice was studied. all lectins administered had been shown previously to suppress immune reactivity. among plant lectins concanavalin a but not lens culinaris or wheat germ agglutinin partially suppressed hyperglycaemia following low-dose streptozotocin. a similar inhibitory effect was found for the staphylococcal enterotoxin b. finally, administration of an immunomodulatory lectin ...	1986	3742941
detection of anomalies in cell-surface carbohydrates on thrombasthenic platelets using 125i-labeled lectins.	the composition of carbohydrates on the surface of platelets from a patient with glanzmann's thrombasthenia and from seven normal donors were determined and compared. to this end, binding studies were performed using nine different purified 125i-labeled lectins; concanavalin a, p-phytohaemagglutinin, wheat germ agglutinin, dolichos biflorus, pisum sativum, ricinus communis ii agglutinin, tetragonolobus purpureus, lens culinaris and soybean agglutinin. these studies show that thrombasthenic plate ...	1986	3633202
thrombospondin in essential thrombocythemia.	essential thrombocythemia is a myeloproliferative disorder characterized by frequent bleeding and thrombotic complications. on a molecular level, two abnormalities of platelet thrombospondin have been identified: abnormal glycosylation of the intact 185,000-dalton chain has been detected and a shortened form of the thrombospondin chain is present. we have used two monoclonal antibodies and lens culinaris lectin to probe the structure of thrombospondin in the platelets from three patients with es ...	1986	3510684
morphology of seedling lentil (lens culinaris medic., fabaceae) as influenced by light intensity and heredity.	lentil seedlings (lens culinaris medic.) were grown out-of-doors, in a shaded greenhouse or in a controlled environment chamber (15 degrees c, 14 hr day under a bank of incandescent and fluorescent lights). bright light promoted branching of seedlings and inhibited internode elongation. internodes formed after transfer of plants from high to low or from low to high light intensity were like those grown in continuous high or low light intensity. branching was enhanced following transfer from high ...	1986	3803992
tissue of origin of elevated alpha-fetoprotein in ataxia-telangiectasia.	although only a single gene exists for alpha-fetoprotein (afp), differential glycosylation generates several different forms and these are associated with different tissues of origin, namely, liver, gastrointestinal tract, and yolk sac. this microheterogeneity of serum afp was studied in seven patients with ataxia-telangiectasia (at) in order to determine the tissue of origin of their elevated afp levels. concanavalin a (con a), lens culinaris agglutinin a (lca-a), and erythroagglutinating phyto ...	1986	2454778
resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells.	a conventional fluorescence microscope was modified to observe the sites of resonance energy transfer (ret) between fluorescent probes in model membranes and in living cells. these modifications, and the parameters necessary to observe ret between membrane-bound fluorochromes, are detailed for a system that uses n-4-nitrobenzo-2-oxa-1,3-diazole (nbd) or fluorescein as the energy donor and sulforhodamine as the energy acceptor. the necessary parameters for ret in this system were first optimized ...	1986	3771633
concanavalin a and lens culinaris agglutinin binding patterns in normal and neoplastic sebaceous epithelium.	formalin-fixed, paraffin-embedded tissue sections of normal skin, sebaceous hyperplasia, nevus sebaceus, sebaceous adenoma, and sebaceous carcinoma were studied by means of biotinylated and fitc conjugated concanavalin a (con a) and lens culinaris agglutinin (lca). at relatively high concentrations of these lectins, all cutaneous epithelial cells were stained. as the concentration of lca was lowered, there was a corresponding decrease in the intensity of staining of all epithelial cells. with lo ...	1986	3771872
comparison of the n-linked glycopeptides of dqw1 and dr1 molecules.	hla class ii molecules have been isolated from a [3h]mannose-labeled gm3104 b lymphoblastoid cell line with the phenotype dqw1, dr1. the dqw1 molecules were purified by affinity to 77-34 igg specifically reactive with the dqw1 specificity. the dr1 molecules were separated into two subsets, dr1a (70 to 80%) and dr1b (20 to 30%), by sequential affinity to 21r5-igg and 21w4-igg sepharose. the alpha- and beta-chains of [3h]mannose-labeled dqw1, dr1a, and dr1b molecules were separated by sds-page and ...	1986	3486905
[lectin binding of psoriatic skin].	the aim of our study was the characterization of epidermal lectin binding pattern in psoriatic vs. non-psoriatic skin in order to reveal possible alterations of the glycocalyx composition of psoriatic keratinocytes. we used fluoroisothiocyanate-labeled lectins of canavalia ensiformis (cona), phaseolus vulgaris (pha), lens culinaris (lca), and helix pomatia (hpa). the binding pattern of psoriatic (involved and non-lesional) skin did not differ from the control samples in cona, pha, and lca. in ps ...	1986	3776279
interactions between lectins and other components of leguminous protein bodies.	previous work from this laboratory had shown that leguminosa seed extracts contain lectin-bound proteins. in the present paper, the isolation of protein bodies from the seeds of 7 leguminosa species (canavalia ensiformis, lens culinaris, pisum sativum, glycine max, sophora japonica, wisteria floribunda and phaseolus vulgaris) is described. protein bodies were characterized microscopically and by their constituents, storage proteins, lectins and some glycosidases. from the protein bodies, lectin- ...	1986	3954886
partial purification and characterization of mrnas encoding glycollate oxidase and catalase.	polyadenylated mrna was prepared from etiolated and greening leaves of lens culinaris and cotyledons of cucumis sativus during the transition from etiolated to photoautotrophic stage. these mrna preparations were used to identify, by translation in vitro, the precursor forms of glycollate oxidase and catalase, both enzymes being markers of microbodies. the level (per fresh weight) of translatable rna coding for glycollate oxidase was found to increase ten fold during the first 3 d of illuminatio ...	1986	24241847
partial purification and characterization of eosinophil chemotactic factors from soluble extract of fasciola species.	eosinophil chemotactic factor (ecf) was partially purified from common liver flukes (japanese strain of fasciola sp) by a combination of anion-exchange chromatography on de52 and gel filtration on sephacryl s-200. the molecular weight of ecf was estimated to be approximately 27,000 by high-pressure liquid chromatography. the ecf was heat labile (56 and 100 c for 30 minutes) and was not bound with lentil-lectin sepharose. the results of isoelectric focusing showed that ecf comprises at least 2 co ...	1986	3946890
the atpase of cholinergic synaptic vesicles is associated with sugars.	the atpase in synaptic vesicles isolated from torpedo californica electric organ can be nearly completely solubilized in octaethyleneglycoldodecyl ether containing buffer where it is stable only in a narrow ph range around neutrality. solubilized atpase adsorbs to sepharose columns containing covalently linked triticum vulgaris, concanavalin a, glycine max, dolichos biflorus, lens culinaris or pisum sativum lectins in a manner responding to cognate sugar block or enzymatic deglycosylation in mos ...	1986	20493054
production and glycosylation of sperm constitutive proteins in the lizard lacerta vivipara. evolution during the reproductive period.	from epididymal fluid samples taken at three different times during the reproductive period (early april, late april, mid-may), the soluble proteins were separated with one dimensional electrophoresis on polyacrylamide gel. their evolution was studied: firstly quantitatively, after staining with coomassie blue, or, for one protein (the "l" protein), by immunodetection; secondly, according to their glycosylation after transfer to nitrocellulose and treatment with a set of labelled lectins: from w ...	1987	3568614
lectin binding patterns in amphibian epidermis.	a battery of 6 different horseradish peroxidase conjugated lectins has been employed for structural localization of glycoconjugates in amphibian epidermis. lens culinaris (lca) lectin stained the basal membrane and gave no significant reaction on the epidermal layers. canavlia ensiformis (con a) and griffonia simplicifolia ii (gs ii) lectins bound the keratinocyte cytoplasm and the basal membrane as well. ulex europaeus i (uea i) lectin had only reactivity with flask cells. griffonia simplifolia ...	1987	3105215
lectin histochemistry of plaques and tangles in alzheimer's disease.	biotinyl derivatives of several lectins and avidin-horseradish peroxidase were used to study the localization of glycoconjugates in amyloid plaques and in neuritic tangles in brains of patients with alzheimer's disease (ad), downs syndrome (ds) and gerstmann-sträussler syndrome (gss). the lectins tested recognize the following residues: beta-d-galactosyl [ricinus communis agglutinin 120, (rca-1) and peanut agglutinin, (pna)]; alpha-d-galactosyl [griffonia simplicifolia agglutinin (gsa)]; alpha-d ...	1987	2955631
in vivo binding partners of the lens culinaris lectin.	the immobilized lectin from the lentil (lens culinaris) specifically binds two fractions out of the l. culinaris seed globulins. both fractions are displaced from the lectin at low ph values. in addition, fraction i fails to interact at high ionic strengths, and fraction ii in the presence of glucose or other lectin-specific sugars. the behaviour in zonal isoelectric precipitation and electrophoretical patterns indicate that both fractions represent subpopulations of the storage proteins. the in ...	1987	3675873
membrane glycophorins of dantu blood group erythrocytes.	glycophorins of erythrocytes of two unrelated individuals who exhibit the dantu blood group phenotype were studied. immunoblots indicated that erythrocytes of each individual contained a complement of a normal alpha-glycophorin (glycophorin a) and a variant n-glycophorin. delta-glycophorin (glycophorin b) was present in one donor's cells but not the other's; the s and n phenotypes of the latter's erythrocytes may derive from the variant glycophorin. the variant glycophorin is of a smaller size, ...	1987	3305497
genetic mapping of an ancient translocation in the genus lens.	segregation of 18 marker genes was monitored in selfed progeny of a lens culinaris × l. ervoides hybrid; five linkage groups were mapped, one of which contained a reciprocal translocation break-point that differentiates between the parents. four markers were found to be linked to the translocation break-point: aco-1 and pgm-2 on one side and gs and got-2 on the other. the gene pairs on both sides of the translocation are not linked in l. culinaris or in l. orientalis. the l. ervoides gene order ...	1987	24241299
close topographical relationship in alpha foetoprotein (afp) between a lens culinaris binding glycan and the epitope recognized by afp-reactive monoclonal antibody, 18h4.	monoclonal antibodies 18h4 and 19f12 against alpha-foetoprotein (afp) were examined by enzyme immunoassay for binding to two forms of afp that were separated on the basis of the reactivity with lentil lectin (lca). lca-binding and lca non-binding afp, coated on a solid phase, reacted with 18h4 but reactivity with the lca-binding species was inhibited by 60% following pretreatment of the afp with lca. the lectin was a very poor inhibitor of binding of 18h4 to the afp which did not interact with l ...	1987	2434121
graviperception of lentil seedling roots grown in space (spacelab d1 mission).	the growth and graviresponsiveness of roots were investigated in lentil seedlings (lens culinaris l. cv. verte du puy) grown (1) in microgravity, (2) on a 1 g centrifuge in space, (3) in microgravity and then placed on the 1 g centrifuge for 3 h, (4) on the ground. dry seeds were hydrated in space (except for the ground control) and incubated for 25 h at 22 degrees c in darkness. at the end of the experiment, the seedlings were photographed and fixed in glutaraldehyde in a biorack glove box. roo ...	1987	11539054
comparisons of labeling efficiency, biological activity and biodistribution among 125i-, 67ga-dtpa-and 67ga-dfo-lectins.	the labeling efficiency, biological activity and biodistribution of 125i labeled and 67ga chelating agent conjugated lectins were investigated. pisum sativum agglutinin (psa) and lens culinaris agglutinin (lch) were efficiently labeled with 67ga using bifunctional chelating agents such as diethylenetriaminepentaacetic acid (dtpa) and deferoxamine (dfo), whereas labeling with 125i was significantly less efficient. the agglutinating activity of these lectins towards ehrlich ascites tumor (eat) cel ...	1987	3428286
quantitation and characterization of human platelet glycoprotein iiia by radioimmunoassay.	glycoprotein iiia was quantitated in human platelets by radioimmunoassay using antisera specific to platelet membranes and purified glycoprotein iiia. glycoprotein iiia and glycoprotein iib were isolated from washed platelets by triton x-114 extraction followed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. radioiodinated glycoprotein iiia was further purified by affinity chromatography on lentil lectin-sepharose 4b. purified glycoprotein iib showed little crossreactiv ...	1987	3828395
changes in the glycosylation pattern during embryonic development of mouse kidney as revealed with lectin conjugates.	distribution of lectin-binding sites in adult and developing mouse kidney was studied with fluorochrome- and peroxidase-coupled lectins. effects of fixation methods on lectin-binding patterns were also compared. un-induced mesenchymal cells and ureter bud of the early metanephros reacted with concanavalin a, lens culinaris, ricinus communis i, and wheat germ agglutinins, whereas binding sites for both soybean and peanut (pna) agglutinins were seen only in ureter bud tissue. on induction, pna pos ...	1987	3794309
efficacy of mung bean (lentil) and pop rice based rehydration solutions in comparison with the standard glucose electrolyte solution.	children with acute diarrhea and moderate dehydration between 3 months and 5 years of age were randomly assigned to receive treatment with standard who oral rehydration solution (ors) (n = 33) and two other solutions in which the 20 g/l glucose was substituted by 50 g/l of pop rice (n = 31) and 60 g/l of mung bean (lentil) powder (n = 29). satisfactory oral rehydration, as assessed clinically and by changes in pcv and total serum solids (tss), was achieved in 90.9% with who ors, 96.8% with pop r ...	1987	3430248
isolation from human chronic-lymphocytic-leukaemia cells of membrane glycoproteins associated with fc-receptor functions. physical parameters and production of polyclonal antibodies.	two membrane glycoproteins that bound immune complexes and inhibited fc-receptor- (fcr-)mediated functions in vitro were purified from human fcr+ chronic-lymphocytic-leukaemia cells. a multi-step purification was developed, consisting essentially in: (i) tween 40 extraction of crude cell membranes; (ii) solubilization of membrane fragments by renex-30; (iii) isolation of glycoproteins by affinity chromatography on lens culinaris haemagglutinin-sepharose; (iv) papain treatment of the eluted glyco ...	1987	3663159
glycoproteins in the whorls of membrane produced by oligodendroglia in culture.	two glycoproteins of 99 kda and 77 kda which exhibit intense binding to wheat germ agglutinin have been purified from the whorls of membrane produced by oligodendroglia in culture. the whorls of membrane were isolated by gradient centrifugation from purified bovine oligodendroglia maintained in culture. the two glycoproteins were solubilized from the membranes using a non-ionic detergent and purified by sephadex lh-60 chromatography, wheat germ agglutinin affinity chromatography, and sds-polyacr ...	1987	3651447
evidence for glycoprotein components of the hepatocellular bile acid transporter.	the hepatocellular transporter, responsible for the uptake of bile acids and some foreign substances, can be shown to contain carbohydrate moieties. the hepatocellular uptake of cholate and phallotoxin is immediately inhibited by addition of wheat-germ agglutinin. concanavalin a and lentil lectin reduce the uptake in a time-dependent manner. apparently sialic acids or n-acetylglucosamine residues are involved in the translocation process. polypeptides (mr 50,000, 54,000) of the above transport s ...	1987	3663657
glycoconjugates in retinoblastoma. a lectin histochemical study of ten formalin-fixed and paraffin-embedded tumours.	the binding of eleven biotin- or peroxidase-coupled lectins with different carbohydrate specificities to tumour tissue and remaining morphologically normal retina was studied in ten formalin-fixed and paraffin-embedded human eyes with retinoblastoma. in undetached retinas, outer and inner segments of photoreceptors bound concanavalin a (cona) as well as lens culinaris (lca), wheat germ (wga) ricinus communis (rcai) and peanut (pna) agglutinins. both nuclear and plexiform layers bound cona, lca a ...	1987	3105166
boar sperm surface glycoproteins: isolation, localization, and temporal expression during spermatogenesis.	boar sperm glycoprotein fractions were isolated by lens culinaris hemagglutinin affinity chromatography of detergent-solubilized ejaculated spermatozoa, followed by preparative sodium dodecyl sulfate (sds) polyacrylamide gel electrophoresis. in order to develop methods for further investigations of the sperm proteins, we proceeded with two of the isolated glycoproteins. antibodies were raised in female rabbits against each of the two sperm glycoproteins. by a combination of immunosorbent chromat ...	1987	3333754
potentiating and suppressive ige-binding factors are expressed by a single cloned gene.	we have investigated expression of an ige-binding factor (ige-bf) cdna in both cos-7 monkey kidney cells and chinese hamster ovary cells. transient expression of the ige-bf clone in either cell type yielded ige-bf, which potentiated an in vitro ige response and had an affinity for lentil lectin. in contrast, when the transient expression experiments were carried out in the presence of tunicamycin, the factors no longer bound to lentil lectin. moreover, ige-bf expressed under these conditions sup ...	1987	3027707
glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro.	we have studied changes in the binding of fluoresceinated lectins to human sperm during in vitro capacitation. we first determined the surface labeling pattern of viable sperm obtained by the swim-up procedure. sperm were labeled with 100 micrograms/ml fitc-conjugated lectin at 4 degrees c for 30 min. we simultaneously used hoechst stain 33258 as a supravital stain to help differentiate surface from intracellular lectin labeling. of 14 lectins studied, six (phytohemagglutinin-e, concanavalin a, ...	1987	3147937
characterization of a rat salivary sialoglycoprotein complex which agglutinates streptococcus mutans.	rat saliva agglutinated streptococcus mutans ingbritt and nctc 10449 and streptococcus sanguis nctc 7864 but not s. mutans nctc 10921, gs 5, or lm 7, streptococcus sobrinus 6715-13 or omz 65, or streptococcus cricetus hs 6, as measured turbidometrically. the specificity of agglutination by rat saliva was the same as that by human saliva. agglutination was associated with a mucin complex (rat salivary agglutinin complex [rs-a]) of sulfated sialoglycoproteins, with a trace of associated lipid and ...	1987	3570462
the chromatographic heterogeneity of rat transferrin on immobilized concanavalin a and lentil lectin.	a procedure was developed for the isolation of the microheterogeneous forms of rat transferrin consisting of anion-exchange and serial lectin affinity chromatographies. by deploying this technique, four to five different anionic species of the protein were detected in plasma. the two major components obtained, which encompassed 92-94% of the plasma transferrin, were further studied by sequential lectin chromatography. the larger of the two, representing 60-63% of plasma transferrin, was bound by ...	1987	3442627
comparative biochemical analysis of purified s-antigen from human, bovine, porcine and rat retina.	highly purified s-antigen from human, bovine, porcine and rat retina was used to ascertain the molecular weights, isoelectric points, amino-acid composition and presence of carbohydrate moieties. all s-antigen preparations comigrate to 50,000 mw on sds-polyacrylamide gels and demonstrate microheterogeneity upon isoelectric focusing. human s-antigen exhibits two bands at ph 5.9 and 5.6, whilst with bovine s-antigen a broad band at ph 5.9 was observed. porcine and rat s-antigen gave four bands, fo ...	1987	3582507
leishmania braziliensis: protein, carbohydrate, and antigen differences between log phase and stationary phase promastigotes in vitro.	when leishmania species are grown in vitro, parasites from the stationary phase differ from those in log phase growth in being more infective and more resistant to complement and macrophage mediated killing. in the present study, log phase and stationary phase promastigotes of leishmania braziliensis panamensis were compared at the molecular level. differences in polypeptide and glycoprotein composition and antigenicity between log and stationary phase promastigotes of l. b. panamensis were dete ...	1987	3582573
litomosoides carinii: characterization of surface carbohydrates of microfilariae and infective larvae.	the carbohydrate moieties of microfilariae (mf) and infective larvae (l3) have been investigated by lectin-binding technique. mf derived from three sources, namely, uteri (in utero), released in vitro from adults and from blood of rodents infected with litomosoides carinii were examined by using fluoresceinated lectins. wheat germ agglutinin (wga) bound to these mf and the binding was inhibited by n-acetyl glucosamine. in addition to wga, concanavalin a (con a) and lentil lectin (lch) bound to i ...	1987	3602835
the dinitrophenyl group as a selective label in high-performance liquid chromatography of peptides.	1-fluoro-2,4-dinitrobenzene can be used to selectively label histidine, tyrosine, and cysteine residues in maleylated proteins. the usefulness of the resulting chromophores for peptide mapping by high-performance liquid chromatography was demonstrated with the lectin from sainfoin (onobrychis viciifolia). the 2,4-dinitrophenyl (dnp) label also can be used in a hydrophobic modulation approach as the mobility of a labeled model peptide changes considerably when its dnp group is removed by thiolysi ...	1987	3605591
analysis of asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells.	the asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells (cll) were studied by sequential lectin affinity chromatography. glycopeptides were isolated from a concanavalin a (cona)-binding glycoprotein fraction prepared from a soluble cll extract. this fraction, which contained 1.8% of the proteins present in the soluble extract, greater than 30 polypeptides revealed by sds-page analysis, and known cell surface antigens such as hla-dr and p85 glycoprotein, must includ ...	1987	3498886
the carbohydrate moieties of suppressor igg-binding factor released by murine t cells.	the carbohydrate moieties of murine igg-binding factor (igg-bf) were studied using lectins binding n-glycosylated sequences such as concanavalin a (con a), lens culinaris agglutinin (lca), and wheat germ agglutinin (wga), and lectins binding o-glycosylated sequences such as peanut agglutinin (pna) and helix pomatia agglutinin (hpa). sources of igg-bf were: (1) supernatants from t2d4, a t cell hybridoma constitutively producing igg-bf, and (2) factor purified by affinity chromatography on rabbit ...	1987	3500399
heterogeneity of human natural killer cells with respect to lectin-binding ability.	the binding ability of peanut agglutinin (pna), lentil agglutinin (len), soybean agglutinin (sba), wheat germ agglutinin (wga), and asparagus pea agglutinin (asp) to human natural killer (nk) cells with the use of the double-marker immunofluorescence technique was studied. for identification of nk cells, vep 13 (dc 16) monoclonal antibody was used. the receptor for pna lectin was shown exclusively after neuraminidase treatment of cells, and vep 13 antigen was neuraminidase resistant. the majorit ...	1987	3295353
evaluation of the hydrogen breath test in man: definition and elimination of the early hydrogen peak.	after ingestion of a non-absorbable carbohydrate breath hydrogen excretion increases early at about 10 minutes, and again later when the ingested carbohydrate enters the caecum. the late rise has been used as a marker of mouth to caecum transit time, but the source of the early rise has not been satisfactorily explained. we studied in 60 healthy volunteers the source and frequency of the early rise in breath hydrogen after ingestion of a non-absorbable carbohydrate. after ingestion of either lac ...	1987	3623219
metabolic stability of the fucose in rat transferrin.	the metabolic behaviour of the chitobiose core fucose that is a natural constituent of a large proportion of rat transferrin molecules was studied in rats comparatively to that of the polypeptide portion of the glycoprotein by using appropriate labels ([3h]fucose and 125i) and affinity chromatographic techniques (lentil-sepharose). no evidence was obtained to suggest that this residue is cleaved from the glycan in significant amounts before removal of the entire glycoprotein for catabolism. simi ...	1987	3653406
bulk purification of a naturally occurring soluble form of rt1-a class i major histocompatibility complex antigens from da rat liver, and studies of specific immunosuppression.	we describe the bulk purification of a water-soluble form of rt1-a class i mhc antigens from aqueous extracts of da liver. using a combination of monoclonal antibody affinity, lentil lectin affinity, and gel permeation chromatography, we were able to obtain large quantities of pure water-soluble rt1-a antigens. typically, from 40 da livers, 0.5 mg of pure antigen with antigen activity equivalent to 7 x 10(9) nucleated da spleen cells was obtained. the water-soluble rt1-a molecule had a discrete ...	1987	3299912
evaluation of structural and secretory glycoconjugates in normal human jejunum by means of lectin histochemistry.	the labelling pattern of eight lectins was studied in jejunal samples from ten normal subjects, in order to define the normal distribution of structural and secretory glycoconjugates in the small bowel. the following lectins were studied by means of a peroxidase technique on formalin-fixed samples: arachis hypogaea, ricinus communis, canavalia ensiformis, lens culinaris, phaseolus vulgaris, triticum vulgaris, ulex europaeus, dolichos biflorus. phaseolus vulgaris reacted with goblet cell mucus th ...	1987	3570880
reconstitution of the glucose transporter from rat skeletal muscle.	as a step in the purification and characterization of the glucose transporter from rat skeletal muscle, we have reconstituted glucose transport activity in liposomes. plasma membranes were prepared from skeletal muscle which display d-glucose reversible binding of cytochalasin b (10 pmol sites/mg protein; kd = 0.3 microm). older rats gave a slightly lower specific activity and much lower yield of sites per g muscle than young rats. glucose transport activity was reconstituted into liposomes by t ...	1987	3586860
characterization of tumor rejection antigen molecules of chemically induced murine colon tumor c-c26.	the molecular nature of a tumor-specific transplantation antigen (tsta) of a chemically induced balb/c mouse colon tumor c-c26 was investigated. the antigen was noncytolytically extracted by 2.5% n-butanol treatment of the cells. crude butanol extract from c-c26, but not from colon tumor c-c51 and fibrosarcoma meth-a of balb/c mice could provide protection against the challenged c-c26 tumor in the transplantation experiment. crude butanol extract from another syngeneic colon tumor c-c36 also ind ...	1987	3581064
gerbich blood group deficiency of the ge:-1,-2,-3 and ge:-1,-2,3 types. immunochemical study and genomic analysis with cdna probes.	human erythrocytes carry several transmembrane glycoproteins, among which the two minor species associated with the blood group gerbich (ge) antigens, gp c and gp d, play pivotal role since they interact with the membrane cytoskeleton and contribute to maintain the normal red cell shape. on the red cells from two categories of homozygous donors lacking the ge determinants (ge:-1,-2,-3 and ge:-1,-2,3), gp c and gp d are missing but instead there is a new glycoprotein, easily detected by sds/polya ...	1987	3595602
glycophorins of human erythroleukemic k562 cells.	glycophorins related to alpha glycophorin, of the human erythrocyte membrane, were isolated from human erythroleukemic k562 cells. the glycophorins were purified using sodium dodecyl sulfate (sds)/trichloroacetic acid fractionation and folch and hot phenol extractions. 0.1-0.2 micrograms was obtained/10(8) cells, or approximately a 15% yield. sds-gel electrophoresis revealed a pattern similar to erythrocyte alpha glycophorin except for the slower mobility of the glycophorin monomer. two populati ...	1987	3606125
lectin histochemistry of mammalian endothelium.	lectin-histochemical studies were performed on formalin-fixed, paraffin-embedded tissues from ten mammalian species to demonstrate the pattern of carbohydrate residues in vascular endothelium. ten different biotinylated lectins were used as probes and avidin-biotin-peroxidase complex (abc) was used as visualant. ricinus communis agglutinin-i (rca-i) and wheat germ agglutinin (wga) stained vascular endothelium in all species. peanut agglutinin (pna) stained vascular endothelium in all species onl ...	1987	3610672
a lectin cytochemical study of glycoconjugates in the human retina.	the binding to morphologically normal human retina of eleven biotin- or peroxidase-coupled lectins with different carbohydrate specificities was studied. eight formalin-fixed and paraffin-embedded eyes were examined. photoreceptor cells bound lens culinaris (lca), wheat germ (wga), peanut (pna) and ricinus communis (rcai) agglutinins, and concanavalin a (cona). the outer segment region was labeled more strongly that the inner segment region, and pna labeled only cones. all these lectins except p ...	1987	3621302
evidence for the glycoprotein nature of vitamin k-dependent carboxylase from rat liver.	the ability of the rat liver microsomal vitamin k-dependent carboxylase and microsomal precursors of prothrombin and other vitamin k-dependent proteins to bind to lectin gels has been determined. under denaturing conditions which dissociate precursor substrates from the carboxylase enzyme, prothrombin precursors and microsomal proteins labeled in gamma-carboxyglutamate residues with [14c]bicarbonate were nearly quantitatively bound to concanavalin a gels. when lentil lectin gels were used, only ...	1987	3801513
enhanced glycosylation of a melanoma cell surface glycoprotein by retinoic acid: carbohydrate chain analysis by lectin binding.	retinoic acid (ra) inhibits the growth of mouse s91-c2 melanoma cells and enhances the glycosylation of a cell surface sialoglycoprotein (gp160). the present study analyzed the binding of 125i-labeled lectins to gp160 within polyacrylamide slab gels after electrophoretic separation of cellular macromolecules. wheat germ agglutinin (wga) and concanavalin a (con a) bound to gp160 of ra-treated cells (ra-gp160) more extensively than to gp160 of control cells (c-gp160). lens culinaris hemagglutinin ...	1987	3435894
antigenic pattern of human brain glycoproteins as described by monoclonal antibodies.	a battery of monoclonal antibodies was raised against a preparation of lentil lectin-binding membrane glycoproteins from human brain. out of 26 established hybridomas, nine produced antibodies against the human thy-1 antigen. for the remaining 17 lines, reactivity with at least six other antigens could be identified after immunoprecipitation and immunoblotting. several of the antigens were di- or trimeric, mainly in the molecular weight range of 60-120 kda. two of the antibodies were reactive wi ...	1987	3543222
separation of carbohydrates and lectins on carbohydrate-immobilized resins.	a few carbohydrate-immobilized resins were prepared by coupling glycamines of mono- and disaccharides to an epoxy-activated methacrylate base, and the elution profiles of various carbohydrates and lectins from columns packed with the resultant resins were compared. the introduction of glucamine, maltamine or lactamine to the methacrylate base had little effect on the separation of stereoisomers of aldoses, though it enabled group separation of pentoses as well as 6-deoxyhexoses from hexoses. on ...	1987	3624387
monoclonal antibody specific for t cell-derived human ige binding factors.	a b cell hybridoma secreting monoclonal antibody against human ige binding factors was obtained by immunization of balb/c mice with partially purified ige binding factors, and fusion of their spleen cells with sp-2/0-ag14 cells. the monoclonal antibody bound all of the 60,000, 30,000, and 15,000 dalton ige binding factors from two t cell hybridomas and those from activated t cells of a normal individual. the antibody bound both ige-potentiating factors, which had affinity for lentil lectin, and ...	1987	2437189
subunit structure of dihydropyridine-sensitive calcium channels from skeletal muscle.	purified dihydropyridine-sensitive calcium channels from rabbit transverse-tubule membranes consist of three noncovalently associated classes of subunits: alpha (167 kda), beta (54 kda), and gamma (30 kda). cleavage of disulfide bonds reveals two distinct alpha polypeptides and an additional component, delta. the alpha 1 subunit, a 175-kda polypeptide that is not n-glycosylated, contains the dihydropyridine binding site, camp-dependent protein kinase phosphorylation site(s), and substantial hydr ...	1987	2440051
class 1 (unique) tumor antigens of human melanoma: partial purification and characterization of the fd antigen and analysis of a mouse polyclonal antiserum.	serum antibodies from melanoma patient fd were previously shown to detect a unique antigenic specificity (fd) expressed only on the autologous tumor cells (sk-mel-131) in culture. the fd determinant is carried by a mr 90,000 glycoprotein (gp90) that binds to concanavalin a but not to lentil lectin or wheat germ agglutinin. after treatment with endo-n-acetylglucosaminidase h, the antigen no longer bound to concanavalin a. these and other properties indicate the gp90 carries mainly high mannose or ...	1987	2445475
an immunoglobulin m monoclonal antibody, recognizing a subset of acetylcholinesterase molecules from electric organs of electrophorus and torpedo, belongs to the hnk-1 anti-carbohydrate family.	an immunoglobulin m (igm) monoclonal antibody (mab elec-39), obtained against asymmetric acetylcholinesterase (ache) from electrophorus electric organs, also reacts with a fraction of globular ache (amphiphilic g2 form) from torpedo electric organs. this antibody does not react with asymmetric ache from torpedo electric organs or with the enzyme from other tissues of electrophorus or torpedo. the corresponding epitope is removed by endoglycosidase f, showing that it is a carbohydrate. the subset ...	1987	2445915
an arg-gly-asp-directed receptor on the surface of human melanoma cells exists in an divalent cation-dependent functional complex with the disialoganglioside gd2.	the disialogangliosides gd2 and gd3 play a major role in the ability of human melanoma cells to attach to arg-gly-asp-containing substrates such as fibronectin and vitronectin, since pretreatment of these cells with monoclonal antibodies to the oligosaccharide of gd2 and gd3 can inhibit their attachment and spreading on such adhesive proteins. this report demonstrates that human melanoma cells (m21) synthesize and express a glycoprotein receptor that shares antigenic epitopes with the vitronecti ...	1987	2443507
tissue-specific n-glycosylation, site-specific oligosaccharide patterns and lentil lectin recognition of rat thy-1.	to examine the extent to which protein structure and tissue-type influence glycosylation, we have determined the oligosaccharide structures at each of the three glycosylation sites (asn-23, 74 and 98) of the cell surface glycoprotein thy-1 isolated from rat brain and thymus. the results show that there is tissue-specificity of glycosylation and that superimposed on this is a significant degree of site-specificity. on the basis of the site distribution of oligosaccharides, we find that no thy-1 m ...	1987	2886334
expression of human extracellular superoxide dismutase in chinese hamster ovary cells and characterization of the product.	a complementary dna clone from human placenta, encoding human extracellular superoxide dismutase (ec-sod; superoxide:superoxide oxidoreductase, ec 1.15.1.1), has recently been isolated and characterized. an expression plasmid, based on the ec-sod complementary dna, was transfected into chinese hamster ovary cells (cho-k1). the transfected cells secreted human ec-sod to the culture medium. the secreted recombinant (r) ec-sod was isolated in high yield with a three-step procedure beginning with im ...	1987	2821539