Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| cloning and expression in yeast of a plant potassium ion transport system. | a membrane polypeptide involved in k+ transport in a higher plant was cloned by complementation of a yeast mutant defective in k+ uptake with a complementary dna library from arabidopsis thaliana. a 2.65-kilobase complementary dna conferred ability to grow on media with k+ concentration in the micromolar range and to absorb k+ (or 86rb+) at rates similar to those in wild-type yeast. the predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to anim ... | 1992 | 1585180 |
| the activation process of arabidopsis thaliana a1 gene encoding the translation elongation factor ef-1 alpha is conserved among angiosperms. | in arabidopsis thaliana, the activation process of the a1 ef-1 alpha gene depends on several elements. using the gus reporter gene, transient expression experiments have shown that mutations of upstream cis-acting elements of the a1 promoter, or the deletion of an intron located within the 5' non-coding region, similarly affect expression in dicot or monocot protoplasts. the results reported here strongly suggest that this 5' intron is properly spliced in zea mays. we show that two trans-acting ... | 1992 | 1600144 |
| arabidopsis thaliana small subunit leader and transit peptide enhance the expression of bacillus thuringiensis proteins in transgenic plants. | the expression of the modified gene for a truncated form of the cryia(c) gene, encoding the insecticidal portion of the lepidopteran-active cryia(c) protein from bacillus thuringiensis var. kurstaki (b.t.k.) hd73, under control of the arabidopsis thaliana ribulose-1,5-bisphosphate carboxylase (rubisco) small subunit ats1a promoter with and without its associated transit peptide was analyzed in transgenic tobacco plants. examination of leaf tissue revealed that the ats1a promoter with its transit ... | 1992 | 1515613 |
| a homolog of escherichia coli reca protein in plastids of higher plants. | studies of chloroplast dna variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. however, no studies have been done of the biochemical pathways involved. using a part of a cyanobacterial reca gene as a probe in southern blots, we have found homologous sequences in total dna from pisum sativum and arabidopsis thaliana and in a cdna library from arabidopsis. a cdna was cloned and sequenced, and its predicted am ... | 1992 | 1518831 |
| a plant cdna that partially complements escherichia coli reca mutations predicts a polypeptide not strongly homologous to reca proteins. | a plant (arabidopsis thaliana) cdna previously selected for its ability to partially complement the uv sensitivity of escherichia coli reca-uvrc-phr- mutants and designated drt100 (dna-damage repair/toleration) was subcloned into a high-copy-number plasmid and expressed via a bacterial promotor. it increased resistance of reca-uvrb-phr- bacteria to mitomycin c and methyl methanesulfonate as well as to uv light. this lack of specificity, and its ability to increase resistance in both uvrb- and uv ... | 1992 | 1518832 |
| cloning and expression of an arabidopsis nitrilase which can convert indole-3-acetonitrile to the plant hormone, indole-3-acetic acid. | from an arabidopsis thaliana cdna expression library, a cdna clone was isolated, characterized and sequenced which, at the amino acid level, resembled the klebsiella ozaenae bromoxynil nitrilase encoded by the bxn gene. the cdna contained a long open reading frame, starting from two possible neighbouring atg codons and capable of encoding 340 or 346 amino acids with calculated molecular masses of 37526 da or 38176 da, respectively. the sequence similarity between the deduced polypeptides from th ... | 1992 | 1555601 |
| arabidopsis alternative oxidase sustains escherichia coli respiration. | glutamyl-trna reductase, encoded by the hema gene, is the first enzyme in porphyrin biosynthesis in many organisms. hemes, important porphyrin derivatives, are essential components of redox enzymes, such as cytochromes. thus a hema escherichia coli strain (sasx41b) is deficient in cytochrome-mediated aerobic respiration. upon complementation of this strain with an arabidopsis thaliana cdna library, we isolated a clone which permitted the sasx41b strain to grow aerobically. the clone encodes the ... | 1992 | 1438286 |
| leucine aminopeptidase from arabidopsis thaliana. molecular evidence for a phylogenetically conserved enzyme of protein turnover in higher plants. | leucine aminopeptidases are exopeptidases which are presumably involved in the processing and regular turnover of intracellular proteins; however, their precise function in cellular metabolism remains to be established. towards this goal, a full-length complementary dna encoding a plant leucine aminopeptidase was isolated from a cdna library of arabidopsis thaliana and sequenced. the nucleotide sequence showed 49.5% identity to the escherichia coli xerb-encoded leucine aminopeptidase. sequence a ... | 1992 | 1555602 |
| multiple resistance to sulfonylureas and imidazolinones conferred by an acetohydroxyacid synthase gene with separate mutations for selective resistance. | the acetohydroxyacid synthase (ahas) gene from the arabidopsis thaliana mutant line gh90 carrying the imidazolinone resistance allele imr1 was cloned. expression of the ahas gene under the control of the camv 35s promoter in transgenic tobacco resulted in selective imidazolinone resistance, confirming that the single base-pair change found near the 3' end of the coding region of this gene is responsible for imidazolinone resistance. a chimeric ahas gene containing both the imr1 mutation and the ... | 1992 | 1557022 |
| a complete cdna for adenine phosphoribosyltransferase from arabidopsis thaliana. | an intact cdna from arabidopsis thaliana for adenine phosphoribosyltransferase (aprt) was isolated and sequenced. the cdna is 729 nucleotides in length and predicts a protein of mr 27,140. the deduced amino acid sequence has been compared with those of other aprts and shown to be most similar to the escherichia coli protein. construction of a molecular tree of the known aprt amino acid sequences indicates the a. thaliana and e. coli aprt sequences form one cluster and the currently available ver ... | 1992 | 1558943 |
| cloning and sequencing of a cdna encoding ascorbate peroxidase from arabidopsis thaliana. | a cdna clone encoding ascorbate peroxidase (ap, ec 1.11.1.11) was isolated from a phage lambda gt11 library of cdna from arabidopsis thaliana by immunoscreening with monoclonal antibodies against the enzyme, and then sequenced. the cdna insert hybridized to a 1.1 kb poly(a)+ rna from leaves of a. thaliana. genomic hybridization suggests that the cdna obtained here corresponds to a single-copy gene. the n-terminal amino acid sequence of arabidopsis ap was determined by protein sequencing of the i ... | 1992 | 1558944 |
| a cold-regulated arabidopsis gene encodes a polypeptide having potent cryoprotective activity. | we previously showed that cor15, a cold-regulated gene from arabidopsis thaliana, encodes a 15 kda polypeptide designated cor15. here we report that cor15 has potent cryoprotective activity in an in vitro cryoprotection assay. specifically, cor15 was very effective in protecting the cold-labile enzyme lactate dehydrogenase against freeze-inactivation; on a concentration basis, it was about 10(6) times more effective than sucrose and 10(2)-10(3) times more effective than other proteins including ... | 1992 | 1567390 |
| genetic consequences of radioactive contamination for populations of arabidopsis. | populations of arabidopsis thaliana (l.) heynh. growing within a 30 km radius of chernobyl and characterized by radioactive contamination levels ranging from 0.02 to 240 mr h-1 were analyzed for the frequency of embryonic lethal mutations in 1987 and 1988. plots that retained high levels of radioactive contamination long after the initial exposure were characterized by a high frequency of mutant plants. plots with low levels of radioactive contamination, which were significantly reduced soon aft ... | 1992 | 1574701 |
| synthesis of the proline analogue [2,3-3h]azetidine-2-carboxylic acid. uptake and incorporation in arabidopsis thaliana and escherichia coli. | azetidine-2-carboxylic acid, the 4-membered ring noranalogue of proline, is regularly used in the study of proline metabolism as well as the study of protein conformation. we prepared d,l-[2,3-3h]azetidine-2-carboxylic acid with an optimized 10% yield from commercially available 4-amino-[2,3-3h]butyric acid. purification was performed by fast-protein liquid chromatography. the biological activity was checked in both arabidopsis thaliana and escherichia coli. the obtained specific activity of 10 ... | 1992 | 1505664 |
| characterization of arabidopsis thaliana telomeres isolated in yeast. | in an effort to learn more about the genomic organization of chromosomal termini in plants we employed a functional complementation strategy to isolate arabidopsis thaliana telomeres in the yeast, saccharomyces cerevisiae. eight yeast episomes carrying a. thaliana telomeric sequences were obtained. the plant sequences carried on two episomes, ypatt1 and ypatt7, were characterized in detail. the telomeric origins of ypatt1 and ypatt7 insert dnas were confirmed by demonstrating that corresponding ... | 1992 | 1508688 |
| potato granule-bound starch synthase promoter-controlled gus expression: regulation of expression after transient and stable transformation. | chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (gbss) and the beta-glucoronidase (gus) reporter gene were used to study gbss expression and regulation. analysis of stable transformants revealed that a gbss promoter sequence of 0.4 kb was sufficient to result in tissue-dependent gus expression: levels in stably transformed microtubers exceeded levels in corresponding leaves by orders of magnitude. gbss-gus constructs could be transiently expresse ... | 1992 | 1515610 |
| gene targeting in arabidopsis thaliana. | gene targeting of a chromosomally integrated transgene in arabidopsis thaliana is reported. a chimeric gene consisting of the promoter of the 35s rna of camv, the polyadenylation signal of the octopine synthase gene and the coding region of the bacterial hygromycin phosphotransferase gene (hpt), which was rendered non-functional by deletion of 19 bp, was introduced into the genome of a. thaliana using agrobacterium-mediated gene transfer. a total of 3.46 x 10(8) protoplasts isolated from 17 inde ... | 1992 | 1310519 |
| complementation of saccharomyces cerevisiae auxotrophic mutants by arabidopsis thaliana cdnas. | an arabidopsis thaliana cdna bank has been constituted in a saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (pgk) promoter and terminator. this bank was used to complement eight s. cerevisiae auxotrophic markers. all of them were corrected. these results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. the cdna complementing the ura1 yeast mutant was sequenced, analysed and shown to encode a dihydroorotic ... | 1992 | 1303803 |
| complementation of the cs dis2-11 cell cycle mutant of schizosaccharomyces pombe by a protein phosphatase from arabidopsis thaliana. | the activities of type i protein phosphatases play a central role in eukaryotic cell cycle control. here, we report the cloning and characterization from the flowering plant arabidopsis thaliana of a cdna clone named pp1-at which is highly homologous to protein phosphatase 1. the deduced amino acid sequence of pp1-at shows that the pp1-at protein is 318 amino acid residues long and has a molecular weight of 35,298 da. the pp1-at protein has strong similarity to all other known protein phosphatas ... | 1992 | 1314161 |
| an alignment of 17 deduced protein sequences from plant, fungi, and ciliate h(+)-atpase genes. | seventeen protein sequences of h(+)-atpases from plants (arabidopsis thaliana, nicotiana plumbaginifolia, lycopersicum esculentum), fungi (saccharomyces cerevisiae, schizosaccharomyces pombe, zygosaccharomyces rouxii, neuropora crassa, candida albicans), and a parasitic ciliate (leishmania donovani) have been aligned. twenty sequence fragments were identified which were conserved in h(+)-, na+/k(+)-, and ca++ plasma membrane-atpases. in addition, a total of 118 residues not located in these frag ... | 1992 | 1328178 |
| identification of an essential tyrosine residue in the catalytic site of a chitinase isolated from zea mays that is selectively modified during inactivation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. | chitinase isolated from zea mays seeds is inactivated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (edc) in the absence of exogenous nucleophiles. oligomers of n-acetylglucosamine,n,n',n",n"'-tetra-n-acetylchitotetraose (glcnac4), and to a lesser extent, n,n',n"-tri-n-acetylchitotriose (glcnac3) and n,n'-di-n-acetylchitobiose (glcnac2) provide partial protection against inactivation by the reagent. an examination of the concentration dependence of the protection afforded by glcnac4 revealed ... | 1992 | 1740436 |
| a phosphoribosylanthranilate transferase gene is defective in blue fluorescent arabidopsis thaliana tryptophan mutants. | an arabidopsis thaliana gene encoding phosphoribosylanthranilate transferase is shown to be the gene that is defective in blue fluorescent trp1 mutant plants. this gene, named pat1, was isolated using an a. thaliana cdna clone that suppressed an escherichia coli trpd(-) mutation. the pat1 coding region is homologous to those for the phosphoribosylanthranilate transferases from many microorganisms. unlike other genes involved in aromatic amino acid biosynthesis in a. thaliana, pat1 appears to be ... | 1992 | 16653032 |
| common identity of substrate binding subunit of vacuolar h-translocating inorganic pyrophosphatase of higher plant cells. | there have been conflicting reports in the literature concerning the polypeptide composition of the vacuolar h(+)-translocating inorganic pyrophosphatase (tonoplast h(+)-ppase) of plant cells. the major subunit(s) of the enzyme have been attributed to polypeptides of relative molecular weight (m(r)) 64,500 (beta vulgaris), 67,000 (beta vulgaris), 73,000 (vigna radiata), and 37,000 to 45,000 (zea mays). here, we reconcile these differences to show, through the combined application of independent ... | 1992 | 16653052 |
| species-dependent variation in the interaction of substrate-bound ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) and rubisco activase. | purified spinach (spinacea oleracea l.) and barley (hordeum vulgare l.) ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase supported 50 to 100% activation of substrate-bound rubisco from spinach, barley, wheat (triticum aestivum l.), soybean (glycine max l.), pea (pisum sativum l.), arabidopsis thaliana, maize (zea mays l.), and chlamydomonas reinhardtii but supported only 10 to 35% activation of rubisco from three solanaceae species, tobacco (nicotiana tabacum l.), petunia (petu ... | 1992 | 16653209 |
| isolation and analysis of the expression of two genes for the 81-kilodalton heat-shock proteins from arabidopsis. | we have cloned and characterized two members of the family of genes for the 81-kilodalton heat-shock proteins from arabidopsis thaliana, hsp81-1 and hsp81-2. comparison of the entire genomic sequence of the hsp81-1 gene with the corresponding full-length cdna previously reported as aths83 (tw conner, pr lafayette, rt nagao, jl key [1990] plant physiol 94: 1689-1695) reveals the presence of three introns of 315, 83, and 88 base pairs. by contrast, analysis of the hsp81-2 genomic and partial cdna ... | 1992 | 16668895 |
| arabidopsis acetohydroxyacid synthase expressed in escherichia coli is insensitive to the feedback inhibitors. | acetohydroxyacid synthase (ahas), the first enzyme unique to the biosynthesis of isoleucine, leucine, and valine, is the target enzyme for several classes of herbicides. the ahas gene from arabidopsis thaliana, including the chloroplast transit peptide, was cloned into the bacterial expression plasmid pkk233-2. the resulting plasmid was used to transform an ahas-deficient escherichia coli strain mf2000. the growth of the mf2000 strain of e. coli was complemented by the functional expression of t ... | 1992 | 16669005 |
| cloning and characterization of an arabidopsis thaliana topoisomerase i gene. | cdna and genomic clones encoding dna topoisomerase i were isolated from arabidopsis thaliana lambdagt11 and lambdafix libraries by low stringency hybridization with a saccharomyces cerevisiae top1 probe. the cdna clones include a 2748-base pair open reading frame predicting an amino acid sequence that is highly homologous to sequences encoded by top1 from yeast and human sources. the sequence of the upstream genomic region reveals two putative tata-like elements and a purine-rich region, but no ... | 1992 | 16669064 |
| hormone-resistant mutants of arabidopsis have an attenuated response to agrobacterium strains. | we have examined the response of the hormone-resistant mutants axr1 and axr2 of arabidopsis thaliana to inoculation by agrobacterium tumefaciens and agrobacterium rhizogenes. our results indicate that recessive mutations in the axr1 gene affect the frequency of tumor formation after inoculation with either agrobacterium strain. in addition, tumors produced on axr1 plants were smaller than those growing on wild-type plants. these results indicate that the product of the axr1 gene is important for ... | 1992 | 16668774 |
| phytoalexin accumulation in arabidopsis thaliana during the hypersensitive reaction to pseudomonas syringae pv syringae. | inoculation of leaves of arabidopsis thaliana (l.) heynh. with the wheat pathogen, pseudomonas syringae pv syringae, resulted in the expression of the hypersensitive reaction and in phytoalexin accumulation. no phytoalexin accumulation was detected after infiltration of leaves with a mutant of p. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, xanthomonas campestris pv campestris; or with 10 millimolar potassium phosphate buffer (ph 6.9). phy ... | 1992 | 16668792 |
| vegetative and seed-specific forms of tonoplast intrinsic protein in the vacuolar membrane of arabidopsis thaliana. | reports from a number of laboratories describe the presence of a family of proteins (the major intrinsic protein family) in a variety of organisms. these proteins are postulated to form channels that function in metabolite transport. in plants, this family is represented by the product of nod26, a nodulation gene in soybean that encodes a protein of the peribacteroid membrane, and tonoplast intrinsic protein (tip), an abundant protein in the tonoplast of protein storage vacuoles of bean seeds (k ... | 1992 | 16668923 |
| expression of constitutive and tissue-specific acyl carrier protein isoforms in arabidopsis. | we have characterized the occurrence and expression of multiple acyl carrier protein (acp) isoforms in arabidopsis thaliana (l.) heynh ecotype columbia. immunoblot analysis of acps from arabidopsis tissues separated by native polyacrylamide gel electrophoresis and 1 molar urea polyacrylamide gel electrophoresis revealed a complex pattern of multiple acp isoforms. all tissues examined (leaves, roots, and seeds) expressed at least three forms of acp. the immunoblot identifications of acp bands wer ... | 1992 | 16668615 |
| polyhydroxybutyrate, a biodegradable thermoplastic, produced in transgenic plants. | polyhydroxybutyrate (phb), a high molecular weight polyester, is accumulated as a storage carbon in many species of bacteria and is a biodegradable thermoplastic. to produce phb by genetic engineering in plants, genes from the bacterium alcaligenes eutrophus that encoded the two enzymes required to convert acetoacetyl-coenzyme a to phb were placed under transcriptional control of the cauliflower mosaic virus 35s promoter and introduced into arabidopsis thaliana. transgenic plant lines that conta ... | 1992 | 17787950 |
| structure and expression of ats1, an arabidopsis thaliana gene homologous to the s-locus related genes of brassica. | genetic and molecular analysis of the self-incompatibility locus (s-locus) of the crucifer brassica has led to the characterization of a multigene family involved in pollen-stigma interactions. while the crucifer arabidopsis thaliana does not have a self-incompatibility system, s-related sequence were detected in this species by cross-hybridization with brassica dna probes. in this paper, we show that an a. thaliana s-related sequence, designated ats1, is expressed specifically in flower buds. s ... | 1992 | 1371578 |
| arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. biochemical and genetic characterization of a plant oleoyl-phosphatidylcholine desaturase. | the overall fatty acid composition of leaf lipids in a mutant of arabidopsis thaliana was characterized by reduced levels of polyunsaturated 18-carbon fatty acids and an increased proportion of oleate as a consequence of a single recessive nuclear mutation. quantitative analysis of the fatty acid composition of individual lipids demonstrated that all the major phospholipids of the extrachloroplast membranes are affected by the mutation, whereas the chlorplast lipids show fatty acid compositions ... | 1992 | 1730697 |
| occurrence of temperature-sensitive phenotypic plasticity in chlorophyll-deficient mutants of arabidopsis thaliana. | a collection of 75 putative mutants with alterations in leaf pigmentation was visually selected from arabidopsis thaliana plants (m(2) generation) grown at 26 degrees c from seeds treated with the mutagen ethylmethanesulfonate. fifty-eight of the plants were found to have chlorophyll contents decreased by at least 10% from the parental columbia ecotype. these plants were screened for chlorophyll content and the ratio of chlorophyll b/a after growth at 20 or 26 degrees c. relative to the parental ... | 1992 | 16668642 |
| cdna sequence analysis and expression of two cold-regulated genes of arabidopsis thaliana. | the dna sequences of cdnas for two cor (cold-regulated) genes of arabidopsis thaliana l. (heyn) were determined. one cdna (approximately 70% full-length) corresponds to a cor gene, designated cor47, that encodes a 47 kda hydrophilic polypeptide. the data indicate that cor47 has amino acid sequence homology with group ii lea (late embryogenesis abundant) proteins, a class of proteins that accumulate late in embryo development. dna sequence analysis of a second cdna (containing the complete protei ... | 1992 | 1731964 |
| cdna and derived amino acid sequence of a cytosolic cu,zn superoxide dismutase from arabidopsis thaliana (l.) heyhn. | 1992 | 1731963 | |
| heavy ion induced mutations in genetic effective cells of a higher plant. | arabidopsis thaliana offers different possibilities for investigating heavy ion induced early and late damage. mutations in genetic effective cells can yield early damage, in the form of reduced vitality of the descending cell-lines and/or late damage, such as mutation induction visible in the following generations. investigation is possible on different levels of ploidy (4n, 2n, n). different genetic effective cells with equal genomes are available. additionally, several different biological en ... | 1992 | 11537049 |
| ultrastructural analysis of organization of roots obtained from cell cultures at clinostating and under microgravity. | data are presented of a comparative analysis on rhizogenesis in the arabidopsis thaliana tissue culture growing in a solid nutrient medium under stationary conditions, clinostatic conditions and microgravity. tissue samples weighing 100 mg. were set in the petri dishes and placed in a horizontal slow clinostat /2 revs/min/. after 14 days of growth they were analyzed. on clinostating the number of roots formed from the callus cells was approximately one half the control. the formed root cap manif ... | 1992 | 11536994 |
| the glucosinolate-degrading enzyme myrosinase in brassicaceae is encoded by a gene family. | a full-length cdna clone (mb3) and three partial clones (ma1, mb1 and mb2) which encode myrosinase (thioglucoside glucohydrolase, ec 3.2.3.1) were isolated from a sinapis alba (white mustard) cdna library. nucleotide sequence analysis of these clones revealed that they are encoded by a gene family. southern blot analysis with gene-specific probes showed that the gene family consists of a least two subfamilies (ma and mb) each with several members both in s. alba and in brassica napus (oilseed ra ... | 1992 | 1731996 |
| isolation and transcriptional competence of three trna(trp) genes from arabidopsis thaliana l. | 1992 | 1731970 | |
| expression of an inward-rectifying potassium channel by the arabidopsis kat1 cdna. | inward-rectifying potassium channels located in the plasma membrane of higher plant and animal cells contribute to cellular homeostasis and excitability. the genes encoding this specific class of k+ channels have not been functionally identified. this report shows that a single messenger rna transcript from the arabidopsis thaliana kat1 complementary dna confers the functional expression of a hyperpolarization-activated k+ channel in xenopus oocytes. the channels encoded by kat1 are highly selec ... | 1992 | 8966547 |
| voltage-dependent ca2+ channels in the plasma membrane and the vacuolar membrane of arabidopsis thaliana. | voltage-dependent ca2+ channels in the plasma membrane and the vacuolar membrane of arabidopsis thaliana have been studied at the single-channel level using the patch-clamp technique. the ca2+ channel in the plasma membrane opened for extracellular ca2+ influx. the ca2+ channel in the vacuolar membrane opened for cytoplasmic ca2+ influx. | 1992 | 1333806 |
| a homoeotic mutant of arabidopsis thaliana with leafy cotyledons. | cotyledons are specialized leaves produced during plant embryogenesis. cotyledons and leaves typically differ in morphology, ultrastructure, and patterns of gene expression. the leafy cotyledon (iec) mutant of arabidopsis thaliana fails to maintain this distinction between embryonic and vegetative patterns of plant development. mutant embryos are phenotypically abnormal, occasionally viviparous, and intolerant of desiccation. mutant cotyledons produce trichomes characteristic of leaves, lack emb ... | 1992 | 17742538 |
| characterization of overexpressed cdnas isolated from a hormone-autonomous, radiation-induced tumor tissue line of arabidopsis thaliana. | to investigate the molecular mechanisms of hormonal control of growth, we constructed a subtracted cdna library enriched for sequences expressed more in a hormone-autonomous, radiation-induced tumor tissue line of arabidopsis thaliana than in normal, hormone-dependent callus. ten cdna clones, which are expressed 1.3- to 10-fold more in the tumor line, were isolated and partially characterized. the clones differ greatly in their level of expression in tumor tissue and in their pattern of expressi ... | 1992 | 16653233 |
| the aba mutant of arabidopsis thaliana (l.) heynh. has reduced chlorophyll fluorescence yields and reduced thylakoid stacking. | it has been shown that the aba mutant of arabidopsis thaliana (l.) heynh. is impaired in epoxy-carotenoid biosynthesis and accumulates the epoxy-carotenoid precursor, zeaxanthin (c.d. rock, j.a.d. zeevaart [1991] proc natl acad sci usa 88: 7496-7499). in addition to providing conclusive evidence for the indirect pathway of abscisic acid biosynthesis from epoxy-carotenoids, the aba mutation offers a powerful means to study the function of xanthophylls (oxygenated carotenoids) in photosynthesis. w ... | 1992 | 16653199 |
| crystal structure of tfiid tata-box binding protein. | the structure of a central component of the eukaryotic transcriptional apparatus, a tata-box binding protein (tbp or tfiid tau) from arabidopsis thaliana, has been determined by x-ray crystallography at 2.6 a resolution. this highly symmetric alpha/beta structure contains a new dna-binding fold, resembling a molecular 'saddle' that sits astride the dna. the dna-binding surface is a curved, antiparallel beta-sheet. when bound to dna, the convex surface of the saddle would be presented for interac ... | 1992 | 1436073 |
| effect of four classes of herbicides on growth and acetolactate-synthase activity in several variants of arabidopsis thaliana. | we have isolated a triazolopyrimidine-resistant mutant csrl-2, of arabidopsis thaliana (l.) heynh. here, we compare csrl-2 with the previously isolated mutants csrl and csr1-1, and with wild-type arabidopsis for responses to members of four classes of herbicides, namely, sulfonylureas, triazolopyrimidines, imidazolinones, and pyrimidyl-oxy-benzoates. two separable herbicide binding sites have been identified previously on the protein of acetolactate synthase (als). here, the mutation giving rise ... | 1992 | 24178380 |
| photophysiology of the elongated internode (ein) mutant of brassica rapa: ein mutant lacks a detectable phytochrome b-like polypeptide. | several phytochrome-controlled processes have been examined in etiolated and light-grown seedlings of a normal genotype and the elongated internode (ein/ein) mutant of rapid-cycling brassica rapa. although etiolated ein seedlings displayed normal sensitivity to prolonged far-red light with respect to inhibition of hypocotyl elongation, expansion of cotyledons, and synthesis of anthocyanin, they displayed reduced sensitivity to prolonged red light for all three of these deetiolation responses. in ... | 1992 | 16653143 |
| detection and partial sequence of phytochrome genes in the ferns anemia phyllitidis (l.)sw (schizaeaceae) and dryopteris filix-mas l. (polypodiaceae) by using polymerase-chain reaction technology. | phytochrome controls several developmental steps during formation and differentiation of the fern gametophyte, including spore germination, morphogenesis of the gametophyte or differentiation of the sexual cells. to obtain information about the amino acid sequence and the regulation of phytochrome expression at the gene level, two degenerated oligonucleotides against well conserved amino acid regions were designed after an optimal alignment of the known phytochrome sequences. these primers were ... | 1992 | 1475322 |
| brain proteins in plants: an arabidopsis homolog to neurotransmitter pathway activators is part of a dna binding complex. | the g box is a well-characterized cis-acting dna regulatory element found in the promoters of several seemingly unrelated plant genes, including the alcohol dehydrogenase (adh) gene of arabidopsis thaliana. using a monoclonal antibody screening approach coupled with electrophoretic mobility shift assays, we have isolated a cdna clone encoding a protein that is part of the in vitro protein/g box complex. the derived amino acid sequence is homologous to a class of proteins in mammalian brains desc ... | 1992 | 1454838 |
| flavonoid-specific staining of arabidopsis thaliana. | crop yields may be threatened by increases in uv-b radiation resulting from depletion of the ozone layer. in higher plants, the presence of flavonols provides a protective mechanism, and we report a novel staining procedure for the visualization of such protectants in plant tissue. it is shown that the proposed technique provides sensitive and specific fluorescence of flavonoids in chlorophyll-bleached tissue of arabidopsis thaliana. | 1992 | 1282347 |
| characterization of a gene that encodes a homologue of protein kinase in arabidopsis thaliana. | cloning and analysis of a gene that encodes a homologue of protein kinase (pk) from arabidopsis thaliana is reported. oligodeoxyribonucleotides (oligos) corresponding to conserved regions in catalytic domains of various pks were used for polymerase chain reaction (pcr) amplification with genomic dna from a. thaliana as template, in an attempt to identify genes encoding pk in plants. we obtained several amplified dna fragments that encoded part of a pk. we screened a genomic dna library of a. tha ... | 1992 | 1446831 |
| characterization of the genes encoding u4 small nuclear rnas in arabidopsis thaliana. | three genes encoding u4 small nuclear rna (u4 snrna) in the higher plant arabidopsis thaliana have been isolated and characterized. two of the genes, atu4.1 and atu4.2, contain all the transcriptional signals known to be essential for u-snrna gene activity in dicot plants: the upstream sequence element (use), the -30 tata box and the downstream 3' end formation sequence. the use and tata elements are centered approximately four helical dna turns apart, a feature characteristic of rna polymerase ... | 1992 | 1287476 |
| blue light activates a specific protein kinase in higher plants. | blue light mediates the phosphorylation of a membrane protein in seedlings from several plant species. when crude microsomal membrane proteins from dark-grown pea (pisum sativum l.), sunflower (helianthus annuus l.), zucchini (cucurbita pepo l.), arabidopsis (arabidopsis thaliana l.), or tomato (lycopersicon esculentum l.) stem segments, or from maize (zea mays l.), barley (hordeum vulgare l.), oat (avena sativa l.), wheat (triticum aestivum l.), or sorghum (sorghum bicolor l.) coleoptiles are i ... | 1992 | 16653043 |
| complementary dna sequence of an adenylate translocator from arabidopsis thaliana. | 1992 | 16653021 | |
| cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from arabidopsis thaliana. | 1992 | 1398114 | |
| cloning and sequence analysis of a cdna clone from arabidopsis thaliana homologous to a proteasome alpha subunit from drosophila. | a cdna clone isolated from an arabidopsis thaliana cell suspension culture library showed considerable similarities to the proteasome 28 kda alpha subunit of drosophila [(1990) gene 90, 235-241]. the 250 amino acid-long protein encoded by arabidopsis tas-g64 clone has important homologies in its primary structure and in the predicted secondary structure with the pros-28.1 clone from drosophila. the only divergence observed between the two sequences is for the 20 c-terminal amino acids. this subu ... | 1992 | 1516703 |
| emf, an arabidopsis gene required for vegetative shoot development. | in higher plants, the transition from the vegetative to the; reproductive state in the shoot meristem initiates flowering. to study this floral transition, constitutively flowering mutant of arabidopsis thaliana ecotype columbia, embryonic flower (emf), was characterized. no vegetative shoots were produced from emf embryos or calli; the shoot apical meristems (sams) in the emf embryos were altered compared to wild-type sams. the mutant sams enlarged precociously and produced inflorescence merite ... | 1992 | 17742537 |
| regeneration of fertile plants from excised immature zygotic embryos of arabidopsis thaliana. | we present protocols for the regeneration of fertile plants from immature zygotic embryos of arabidopsis thaliana ecotype "zürich" either directly by multiple shoot formation from the apical region or indirectly by shoot induction from embryo derived calli. the regeneration efficiency by "multiple shooting" depended on the developmental stage of the cultured embryos and ranged from 15% for early heart shaped to 90% for early torpedo shaped and further developed embryos. 85% callus induction was ... | 1992 | 24201731 |
| efficient transformation of arabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypes and agrobacterium strains. | the efficiency of agrobacterium-mediated transformation of arabidopsis thaliana was compared with different organs, arabidopsis ecotypes, and agrobacterium strains. efficiency of shoot regeneration was examined using hypocotyl, cotyledon and root explants prepared from young seedlings. hypocotyl expiants had the highest regeneration efficiency in all of the four arabidopsis ecotypes tested, when based on a tissue culture system of callus-inducing medium (cim: valvekens et al. 1988) and shoot-ind ... | 1992 | 24201721 |
| the expression pattern of the tonoplast intrinsic protein gamma-tip in arabidopsis thaliana is correlated with cell enlargement. | the vacuolar membrane (tonoplast) contains an abundant intrinsic protein with six membrane-spanning domains that is encoded by a small gene family. different isoforms of tonoplast intrinsic protein (tip) are expressed in different tissues or as a result of specific signals. using promoter-beta-glucuronidase (gus) fusions and in situ hybridization, we have examined the expression of gamma-tip in arabidopsis thaliana. gus staining of plants transformed with promoter-gus fusions showed that gamma-t ... | 1992 | 16653178 |
| the nad4l gene is encoded between exon c of nad5 and orf25 in the arabidopsis mitochondrial genome. | in the arabidopsis thaliana mitochondrial genome the gene coding for subunit 4l of nadh dehydrogenase (nad4l) is located between exon c of the nad5 open reading frame and orf25. the physical proximity of these genes further supports the trans-splicing model for assembly of the nad5 mrna. some transcripts comprise the nad4l open reading frame cotranscribed with nad5 exon c and orf25, while other mrnas contain only nad4l and orf25. both open reading frames for nad4l and orf25 are edited in several ... | 1992 | 1494348 |
| mitochondrial rps14 is a transcribed and edited pseudogene in arabidopsis thaliana. | we have isolated and analysed a 2 kb region of the mitochondrial genome of arabidopsis thaliana (columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from oenothera berteriana and vicia faba, as well as with an open reading frame (orf) located upstream of the nad3 locus in o. berteriana. the rps14 locus is present as a single copy in the a. thaliana mt genome and has a translational stop codon located near the initiation cod ... | 1992 | 1463850 |
| arabidopsis thaliana carbonic anhydrase: cdna sequence and effect of co2 on mrna levels. | a full-length cdna clone encoding carbonic anhydrase was isolated from an arabidopsis thaliana (columbia) leaf library. comparison of the derived amino acid sequence obtained from this clone with those of pea and spinach reveals a considerable degree of identity. the carbonic anhydrase cdna was used to probe the level of rna encoding this protein in the leaves of plants grown in elevated co2 (660 ppm). we have found that under these conditions the steady-state level of carbonic anhydrase mrna wa ... | 1992 | 1463847 |
| the expression of a rab-related gene, rab18, is induced by abscisic acid during the cold acclimation process of arabidopsis thaliana (l.) heynh. | we have isolated a rab-related (responsive to aba) gene, rab18 from arabidopsis thaliana. the gene encodes a hydrophilic, glycine-rich protein (18.5 kda), which contains the conserved serine- and lysine-rich domains characteristic of similar rab proteins in other plant species. the rab18 mrna accumulates in plants exposed to low temperature, water stress or exogenous aba but not in plants subjected to heat shock. this stress-related accumulation of the rab18 mrna is markedly decreased in the aba ... | 1992 | 1463831 |
| rnp-t, a ribonucleoprotein from arabidopsis thaliana, contains two rnp-80 motifs and a novel acidic repeat arranged in an alpha-helix conformation. | we have isolated a 1148 bp long cdna clone encoding an rna-binding protein in arabidopsis. several partial cdna clones were isolated by screening an arabidopsis lambda gt11 expression library for the binding of dna. one of these clones was used as a probe to isolate a full-length clone. the 329 amino acid protein, termed rnp-t, contains in its carboxy terminus two adjacent rnp-80 motifs, a previously described 80 amino acid long conserved putative rna-binding domain. each rnp-80 motif includes b ... | 1992 | 1463823 |
| effects of the axr2 mutation of arabidopsis on cell shape in hypocotyl and inflorescence. | the axr2 mutation of arabidopsis thaliana (l.) heynh. confers resistance to the plant growth hormones auxin, ethylene and abscisic acid. in addition, mutant plants have a pronounced dwarf phenotype and display defects in both shoot and root gravitopism. to further characterize this mutant we have determined the phenotype of both dark- and light-grown mutant seedlings. we find that the height of axr2 hypocotyls is reduced in dark conditions compared with wild-type seedlings and that both dark- an ... | 1992 | 24178265 |
| gibberellin is required for flowering in arabidopsis thaliana under short days. | mutants of arabidopsis thaliana deficient in gibberellin synthesis (ga1-3 and ga1-6), and a gibberellin-insensitive mutant (gai) were compared to the wild-type (wt) landsberg erecta line for flowering time and leaf number when grown in either short days (sd) or continuous light (cl). the ga1-3 mutant, which is severely defective in ent-kaurene synthesis because it lacks most of the ga1 gene, never flowered in sd unless treated with exogenous gibberellin. after a prolonged period of vegetative gr ... | 1992 | 16652976 |
| abscisic acid elicits the water-stress response in root hairs of arabidopsis thaliana. | water stress has been shown to cause root hairs to become short and bulbous. because abscisic acid (aba) mediates a variety of water-stress responses, we investigated the response of arabidopsis thaliana root hairs to aba. when wild-type root hairs were treated with aba, they exhibited the water-stress response. the arabidopsis mutants abi1 and abi2, which are insensitive to aba at the seedling stage, did not display the root hair response. these data suggest that aba may mediate the response of ... | 1992 | 16652949 |
| cloning, genetic mapping, and expression analysis of an arabidopsis thaliana gene that encodes 1-aminocyclopropane-1-carboxylate synthase. | a genomic clone of one member of the arabidopsis thaliana (l.) heynh. 1-aminocyclopropane-1-carboxylate (acc) synthase (s-adenosyl-l-methionine methylthioadenosine-lyase, ec 4.4.1.14) gene family (at-acc1) was isolated and sequenced. a region of homology was found in the 5'-untranslated region with the promoter of a zucchini and a tomato acc synthase gene. comparison of its primary structure with other acc synthases revealed conservation of seven peptide regions as well as similarity with 11 ami ... | 1992 | 1357670 |
| characterization of a cold-regulated wheat gene related to arabidopsis cor47. | the cdna clone pwg1 represents a gene, designated cor39, from triticum aestivum l. cv winoka (a winter wheat) that is related to a cold-regulated arabidopsis thaliana l. (heyn) gene, cor47. in vitro transcription/translation experiments in conjunction with dna sequence analysis indicated that cor39 encodes a hydrophilic polypeptide of 39 kd (isoelectric point of 7.5), designated cor39. the polypeptide is composed primarily of two sequences, each of which is repeated six times. one sequence, whic ... | 1992 | 16653076 |
| alpha-tubulin gene family of maize (zea mays l.). evidence for two ancient alpha-tubulin genes in plants. | among 81 alpha-tubulin cdna clones prepared from rna from maize seedling shoot, endosperm and pollen, we identified six different alpha-tubulin coding sequences. the dna sequence analysis of coding and non-coding regions from the clones showed that they can be classified into three different alpha-tubulin gene subfamilies. genes within each subfamily encode proteins that are 99 to 100% identical in amino acid sequence. deduced amino acid sequence identity between genes in different subfamilies r ... | 1992 | 1522603 |
| the isolation of apparently homoplastidic mutants induced by a nuclear recessive gene in arabidopsis thaliana. | the nuclear recessive gene, chm1, of arabidopsis thaliana is a imitator that induces a variety of plastid alterations giving rise to mixed cells and variegated leaves. the variegation is maternally transmitted but chm1 is transmitted in a mendelian fashion (rédei 1973; rédei and plurad 1973). in order to characterize the different types of plastid alterations induced by chm1, isolating homoplastidic lines, each apparently containing one type of mutant plastid in its cells, was essential since su ... | 1992 | 24201493 |
| structure and sequence of a dehydrin-like gene in arabidopsis thaliana. | 1992 | 1623199 | |
| an arabidopsis thaliana gene with sequence similarity to the s-locus receptor kinase of brassica oleracea: sequence and expression. | primary signal transduction plays a vital role in the way plants react to environmental and developmental signals. we report the sequence and expression of a putative receptor kinase gene, ark1, in arabidopsis thaliana that may be important in this regard. this arabidopsis gene encodes a transmembrane protein with a cytoplasmic kinase catalytic domain, a transmembrane region, and an extracellular domain with sequence similarity to the secreted s-locus glycoprotein (slg) gene of brassica oleracea ... | 1992 | 16668863 |
| development of an efficient two-element transposon tagging system in arabidopsis thaliana. | modified ac and ds elements, in combination with dominant markers (to facilitate monitoring of excision, reinsertion and segregation of the elements) were introduced into arabidopsis thaliana ecotype landsberg erecta. the frequencies of somatic and germinal transactivation of the ds elements were monitored using a streptomycin resistance assay. transactivation was significantly higher from a stable ac (sac) carrying a 537 bp deletion of the cpg-rich 5' untranslated leader of the transposase mrna ... | 1992 | 1320189 |
| genes encoding a histone h3.3-like variant in arabidopsis contain intervening sequences. | two genes encoding a particular h3 histone variant were isolated from arabidopsis thaliana. these genes differ from the h3 genes previously cloned from arabidopsis and other plants by several interesting properties: (1) the two genes are located close to each other; (2) their coding regions are interrupted by two or three small introns, the two closest to the initiation codon being located at the same place in the two genes; (3) another, long intron is located in the 5'-untranslated region just ... | 1992 | 1593639 |
| cadmium-sensitive mutants of arabidopsis thaliana. | a screening procedure for identifying cd-sensitive mutants of arabidopsis thaliana is described. with this procedure, two cd-sensitive mutants were isolated. these represent independent mutations in the same locus, referred to as cad1. genetic analysis has shown that the sensitive phenotype is recessive to the wild type and segregates as a single mendelian locus. crosses of the mutant to marker strains showed that the mutation is closely linked to the tt3 locus on chromosome 5. in addition to cd ... | 1992 | 16652930 |
| genetic separation of phototropism and blue light inhibition of stem elongation. | blue light-induced regulation of cell elongation is a component of the signal response pathway for both phototropic curvature and inhibition of stem elongation in higher plants. to determine if blue light regulates cell elongation in these responses through shared or discrete pathways, phototropism and hypocotyl elongation were investigated in several blue light response mutants in arabidopsis thaliana. specifically, the blu mutants that lack blue light-dependent inhibition of hypocotyl elongati ... | 1992 | 11538049 |
| spatial pattern of cdc2 expression in relation to meristem activity and cell proliferation during plant development. | the p34 protein kinase encoded by the cdc2 gene is a key component of the eukaryotic cell cycle required for the g1- to s-phase transition and entry into mitosis. to study the regulation of plant meristem activity and cell proliferation, we have examined the tissue-specific accumulation of cdc2 transcripts in arabidopsis thaliana and the related crucifer radish (raphanus sativus) by in situ hybridization using a. thaliana cdc2 cdna sequences as a probe. cdc2 transcripts accumulated in leaf primo ... | 1992 | 1502145 |
| light-independent developmental regulation of cab gene expression in arabidopsis thaliana seedlings. | we found a transient increase in the amount of mrna for four nuclear genes encoding chloroplast proteins during early development of arabidopsis thaliana. this increase began soon after germination as cotyledons emerged from the seed coat; it occurred in total darkness and was not affected by external factors, such as gibberellins or light treatments used to stimulate germination. three members of the cab gene family and the rbcs-1a gene exhibited this expression pattern. because timing of the i ... | 1992 | 1380166 |
| subcellular localization of ubiquitin and ubiquitinated proteins in arabidopsis thaliana. | ubiquitin is a highly conserved, 76-amino acid, eukaryotic protein. its widely accepted role as a proteolytic cofactor depends on its unique ability to covalently ligate to other cellular proteins. while there is good evidence for the existence of such ubiquitinated proteins in the cytosolic and nuclear compartments, relatively little is known about the presence of free ubiquitin and ubiquitinated proteins in other subcellular compartments. this is especially true of higher plants, which have no ... | 1992 | 1322398 |
| spectral-dependence of light-inhibited hypocotyl elongation in photomorphogenic mutants of arabidopsis: evidence for a uv-a photosensor. | photon fluence rate-response curves at different wavelengths were generated for the light-induced inhibition of hypocotyl elongation in seedlings of wildtype and photomorphogenic mutants of arabidopsis thaliana. (l.) heynh. treatment of wild-type seedlings with continuous low-fluence-rate light (< 1.0 μmol photons · m(-2) · s(-1)) induced some inhibition of hypocotyl elongation at all wavelengths tested, with maximum inhibition in blue light. at higher fluence rates, inhibition reached a maximum ... | 1992 | 24178206 |
| nucleotide sequence of a cdna for catalase from arabidopsis thaliana. | 1992 | 16669103 | |
| methyl jasmonate inhibition of root growth and induction of a leaf protein are decreased in an arabidopsis thaliana mutant. | jasmonic acid and its methyl ester, methyl jasmonate (meja), are plant signaling molecules that affect plant growth and gene expression. primary root growth of wild-type arabidopsis thaliana seedlings was inhibited 50% when seedlings were grown on agar medium containing 0.1 m meja. an ethyl methanesulfonate mutant (jar1) with decreased sensitivity to meja inhibition of root elongation was isolated and characterized. genetic data indicated the trait was recessive and controlled by a single mendel ... | 1992 | 11607311 |
| fiddlehead: an arabidopsis mutant constitutively expressing an organ fusion program that involves interactions between epidermal cells. | in most circumstances plant epidermal cells do not respond to surface contact with adjacent plant parts. we have identified and characterized a mutant of arabidopsis thaliana, designated fiddlehead, where lateral appendages of the shoot fuse with one another. while fusion between floral organs is most frequent, leaf fusions also occur. using scanning and transmission electron microscopy, we show that adhesion takes place between epidermal cells and does not involve cytoplasmic union. we also sho ... | 1992 | 1644226 |
| sequence of the fourth and fifth photosystem ii type i chlorophyll a/b-binding protein genes of arabidopsis thaliana and evidence for the presence of a full complement of the extended cab gene family. | a second locus (lhb1b) encoding photosystem ii type i chlorophyll a/b-binding (cab) polypeptides was identified in arabidopsis thaliana. this locus carries two genes in an inverted orientation. the predicted sequences of the polypeptides encoded by these two genes show substantial divergence in their amino termini relative to each other and to the proteins encoded by the three lhb1 cab genes previously characterized [10], but little divergence within the predicted primary structure of the mature ... | 1992 | 1643279 |
| three-dimensional structure of a ubiquitin-conjugating enzyme (e2). | the x-ray crystal structure of a recombinant ubiquitin-conjugating enzyme (e2) encoded by the ubc1 gene of the plant arabidopsis thaliana has been determined with the use of multiple isomorphous replacement techniques and refined at 2.4-a resolution by simulated annealing and restrained least-squares. this e2 is an alpha/beta protein, with four alpha-helices and a four-stranded antiparallel beta-sheet. the nh2 and cooh termini, which may be important for interaction with other enzymes and substr ... | 1992 | 1321826 |
| fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. | medium-chain fatty acids (fas), found in storage lipids of certain plants, are an important renewable resource. seeds of undomesticated california bay accumulate laurate (12:0), and a 12:0-acyl-carrier protein thioesterase (bte) has been purified from this tissue. sequencing of bte enabled the cloning of a complementary dna coding for a plastid-targeted preprotein. expression of the complementary dna in the seeds of arabidopsis thaliana resulted in bte activity, and medium chains accumulated at ... | 1992 | 1621095 |
| ion channels in arabidopsis plasma membrane : transport characteristics and involvement in light-induced voltage changes. | white light (25 watts per square meter) induced an increase in plasma membrane k(+)-channel activity and a 30- to 70-millivolt transient membrane depolarization (completed in 2-3 minutes) in arabidopsis thaliana leaf mesophyll cells. transport characteristics of three types of ion channels in the plasma membrane were determined using inside-out patches. with 220 millimolar k(+) on the cytoplasmic side of the patch and 50 millimolar k(+) in the pipette, (220/50 k), the open-channel current-voltag ... | 1992 | 16668890 |
| establishment of a gene tagging system in arabidopsis thaliana based on the maize transposable element ac. | an ac-derived, two-component transposable element system has been developed and analyzed with respect to its use in arabidopsis thaliana. this system consists of an immobilized ac element ("ac clipped wing", accl) as the source of transactivating transposase and a nonautonomous "ds" element, dsa, which is inserted into a chimaeric neomycinphosphotransferase gene used as excision marker. after separate introduction of acc1 and dsa into arabidopsis thaliana, progeny analysis of crosses between fiv ... | 1992 | 24203197 |
| calcium influx at the tip of growing root-hair cells of arabidopsis thaliana. | the role of extracellular ca(2+) in root-hair tip growth has been investigated in arabidopsis thaliana (l.) heynh. root-hair length was found to be dependent on the concentration of ca(2+) in the growth medium, with maximum length achieved at [ca(2+)] of 0.3-3.0 mm. using a non-intrusive calcium-specific vibrating microelectrode, an extracellular ca(2+) gradient was detected at the tips of individual growing root-hair cells. the direction of the gradient indicated a net influx of ca(2+) into roo ... | 1992 | 24178139 |
| chloroplast division and expansion is radically altered by nuclear mutations in arabidopsis thaliana. | we have isolated three mutants of arabidopsis thaliana in which there is a sevenfold change in chloroplast number in fully expanded leaf mesophyll cells and increases and decreases in chloroplast number are compensated for by changes in chloroplast size. the changes are stably inherited in sexual crosses for three generations and mutant phenotypes are effected by changes at single recessive nuclear loci, termed arc loci. this is the first report of large, stably inherited changes in chloroplast ... | 1992 | 16668963 |
| structure and expression of kin2, one of two cold- and aba-induced genes of arabidopsis thaliana. | we report the isolation of the second member, kin2, of a family of two cold-inducible genes of arabidopsis thaliana. the proteins corresponding to the two genes have similarities to the small antifreeze proteins from winter flounder. kin1 and kin2 are organized in a close tandem array in the genome of a. thaliana. both have three exons separated by introns with approximately the same length and location. the coding regions are highly conserved while the introns and especially the 3' flanking seq ... | 1992 | 1627780 |
| improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. | microtubules are important in plant growth and development. localizing microtubules in sectioned material is advantageous because it allows any tissue of interest to be studied and it permits the positional relations of the cells within the organ to be known. we describe here a method that uses semi-thin (0.5-2 μm) sections of material embedded in butyl-methylmethacrylate, to which 10 mm dithiothreitol was added. after removing the embedding material and using indirect immunofluorescence stainin ... | 1992 | 24178082 |
| dna sequence analysis of a complementary dna for cold-regulated arabidopsis gene cor15 and characterization of the cor 15 polypeptide. | previous studies have indicated that changes in gene expression occur in arabidopsis thaliana l. (heyn) during cold acclimation and that certain of the cor (cold-regulated) genes encode polypeptides that share the unusual property of remaining soluble upon boiling in aqueous solution. here, we identify a cdna clone for a cold-regulated gene encoding one of the "boiling-stable" polypeptides, cor15. dna sequence analysis indicated that the gene, designated cor15, encodes a 14.7-kilodalton hydrophi ... | 1992 | 16668917 |
| isolation and characterization of a mutant of arabidopsis thaliana resistant to alpha-methyltryptophan. | mutants of arabidopsis thaliana have been selected for resistance to growth inhibition at the seedling stage by alpha-methyltryptophan (amt). one mutant, amt-1 has been characterized in detail. the appearance and growth rate of the mutant in the absence of the inhibitor are similar to wild type, both as plants and callus. however, mutant plant growth is unaffected by 25 micromolar amt and mutant callus growth by 50 micromolar amt, concentrations that completely inhibit the growth of wild-type pl ... | 1992 | 16668861 |
| a new homeobox-leucine zipper gene from arabidopsis thaliana. | we have isolated a homeobox-containing gene from arabidopsis thaliana using a degenerate oligonucleotide probe corresponding to the most conserved region of the homeodomain. this strategy has been used previously to isolate homeobox-containing genes from caenorhabditis, and recently from a. thaliana. the arabidopsis genes have an unusual structure in that they have a leucine zipper motif adjacent to the carboxy terminal region of the homeo domain, a feature not found in homeobox-containing genes ... | 1992 | 1349836 |
| a role for membrane lipid polyunsaturation in chloroplast biogenesis at low temperature. | two different mutants of arabidopsis thaliana deficient in chloroplast membrane lipid polyunsaturation were indistinguishable in appearance from the wild-type when grown at 22 degrees c. by contrast, leaf tissues of the mutants that developed during growth at 5 degrees c were chlorotic, whereas the wild type was not. this is the first direct evidence that chloroplast lipid polyunsaturation contributes to low-temperature fitness. chloroplasts from mutant lines grown at 5 degrees c were much small ... | 1992 | 16668849 |