Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| comparative proteomics of a lycopene-accumulating mutant reveals the important role of oxidative stress on carotenogenesis in sweet orange (citrus sinensis [l.] osbeck). | a spontaneous sweet orange (citrus sinenesis [l.] osbeck) mutant 'hong anliu' is of high value due to lycopene accumulation in the pulp. in this study, we analyzed the proteomic alterations in the pulp of 'hong anliu' versus its wild type (wt) at four maturing stages by using 2-de combined with maldi-tof-tof ms. among the 74 differentially expressed proteins identified, the majority are predicted to be involved in stress response, carbohydrate/energy metabolism and regulation, or protein fate, m ... | 2009 | 19834898 |
| proteome analysis of citrus sinensis l. (osbeck) flesh at ripening time. | a combination of 2-de and lc-msms approaches was used to identify the differentially expressed proteome of a pigmented sweet orange (citrus sinensis, cv. moro) in comparison with a common cultivar (cadenera) at ripening time. the comparison of the protein patterns of moro and cadenera showed 64 differential expressed protein spots. fifty-five differentially expressed proteins were identified. proteins were classified according to their putative function and known biosynthetic pathways. most of t ... | 2009 | 19775598 |
| identification, recombinant expression, and biochemical characterization of a flavonol 3-o-glucosyltransferase clone from citrus paradisi. | glucosylation is a predominant flavonoid modification reaction affecting the solubility, stability, and subsequent bioavailability of these metabolites. flavonoid glycosides affect taste characteristics in citrus making the associated glucosyltransferases particularly interesting targets for biotechnology applications in these species. in this work, a citrus paradisi glucosyltransferase gene was identified, cloned, and introduced into the pet recombinant protein expression system utilizing prime ... | 2009 | 19733370 |
| phellodendron and citrus extracts benefit joint health in osteoarthritis patients: a pilot, double-blind, placebo-controlled study. | the objective of this clinical study was to assess the potential benefit of a dietary supplement, np 06-1, on joint health in overweight and normal weight adults diagnosed with osteoarthritis. | 2009 | 19682376 |
| seasonal population dynamics of homalodisca vitripennis (hemiptera: cicadellidae) in sweet orange trees maintained under continuous deficit irrigation. | a 2-yr study was conducted in a citrus orchard (citrus sinensis l. osbeck cultivar valencia) to determine the influence of plant water stress on the population dynamics of glassy-winged sharpshooter, homalodisca vitripennis (germar). experimental treatments included irrigation at 100% of the crop evapotranspiration rate (et(c)) and continuous deficit-irrigation regimens at 80 and 60% et(c). microclimate and plant conditions monitored included temperature and humidity in the tree canopy, leaf sur ... | 2009 | 19610409 |
| pattern recognition and genetic algorithms for discrimination of orange juices and reduction of significant components from headspace solid-phase microextraction. | orange (citrus sinensis l.) juice comprises a complex mixture of volatile components that are difficult to identify and quantify. classification and discrimination of the varieties on the basis of the volatile composition could help to guarantee the quality of a juice and to detect possible adulteration of the product. | 2009 | 19609881 |
| thermal degradation kinetics of anthocyanins from blood orange, blackberry, and roselle using the arrhenius, eyring, and ball models. | anthocyanin stability was assessed over temperatures ranging from 30 to 90 degrees c for seven products: blood orange juice [citrus sinensis (l.) osbeck]; two tropical highland blackberry juices (rubus adenotrichus schlech.), one with high content and the other with low content of suspended insoluble solids (sis); and four roselle extracts (hibiscus sabdariffa l.). the blackberry juice showed the highest content of anthocyanins with 1.2 g/l (two times less in the roselle extracts and 12 times le ... | 2009 | 19545116 |
| efficacy of essential oils from edible plants as insecticides against the house fly, musca domestica l. | the compositions of 12 essential oils (eos) obtained by hydrodistillation of edible fruits and herbs were analyzed by gas chromatography/mass spectroscopy (gc/ms). the insecticidal activity of each oil against the house fly musca domestica was evaluated by placing flies in a glass jar with a screw cap that held a piece of eo-treated cotton yarn. the dose necessary to kill 50% of flies (lc(50)) in 30 min was determined at 26 +/- 1 degrees c. twelve eos and 17 individual terpenes were assayed agai ... | 2009 | 19471213 |
| gene isolation and expression analysis of two distinct sweet orange [citrus sinensis l. (osbeck)] tau-type glutathione transferases. | glutathione s-transferases (gsts) represent a multifunctional family of enzymes grouped into four main classes (tau, phi, theta, and zeta) conjugating endobiotic and xenobiotic compounds to glutathione. in plants, this is considered to be a crucial step in the detoxification process as the s-glutathionylated metabolites are tagged for vacuolar sequestration. in this work, we have isolated two glutathione s-transferases belonging to the tau class gsts from sweet orange leaves. the cdna clones con ... | 2009 | 19422890 |
| influence of spray equipment and water volume on coverage of citrus and control of citricola scale, coccus pseudomagnoliarum (hemiptera: coccidae) with mineral oil. | a trial was conducted in a commercial citrus sinensis l. variety 'washington' navel orange orchard to compare the coverage and efficacy against citricola scale coccus pseudomagnoliarum (kuwana) (hemiptera: coccidae) of 45.5 liters/ha of an nc24 agricultural mineral oil treatment applied by two different methods: a conventional air blast sprayer and a rotary atomizer. three water volumes (2,340, 4,680, and 7,020 liters/ha) were applied with the air blast sprayer to determine the optimal spray vol ... | 2009 | 19253648 |
| transcriptome analysis of a spontaneous mutant in sweet orange [citrus sinensis (l.) osbeck] during fruit development. | bud mutations often arise in citrus. the selection of mutants is one of the most important breeding channels in citrus. however, the molecular basis of bud mutation has rarely been studied. to identify differentially expressed genes in a spontaneous sweet orange [c. sinensis (l.) osbeck] bud mutation which causes lycopene accumulation, low citric acid, and high sucrose in fruit, suppression subtractive hybridization and microarray analysis were performed to decipher this bud mutation during frui ... | 2009 | 19218315 |
| the phytochemical analysis and antioxidant activity assessment of orange peel (citrus sinensis) cultivated in greece-crete indicates a new commercial source of hesperidin. | the flavonoid content of several methanolic extract fractions of navel orange peel (flavedo and albedo of citrus sinensis) cultivated in crete (greece) was first analysed phytochemically and then assessed for its antioxidant activity in vitro. the chemical structures of the constituents fractionated were originally determined by comparing their retention times and the obtained uv spectral data with the available bibliographic data and further verified by detailed lc-dad-ms (esi+) analysis. the m ... | 2009 | 18823075 |
| involvement of polyamine biosynthesis in somatic embryogenesis of valencia sweet orange (citrus sinensis) induced by glycerol. | culture of citrus sinensis embryogenic callus on the embryo-inducing medium (eim) containing glycerol gave rise to a large number of embryos, whereas very few embryos were observed on the callus growth medium (cgm). in the current paper, attempts were made to investigate whether polyamine biosynthesis was involved in glycerol-mediated somatic embryogenesis. quantification of free polyamines by high-performance liquid chromatography showed that the cultures on eim had less putrescine than those o ... | 2009 | 18448195 |
| desiccation and freezing tolerance of embryonic axes from citrus sinensis [l.] osb. pretreated with sucrose. | embryonic axes of citrus sinensis l. were successfully cryopreserved. while fully hydrated unfrozen axes germinated 100%, survival decreased as axes water content dropped, and total loss of viability was observed when the water content dropped to 0.04 and 0.10 mg h2o/mg dry mass, for axes without and with sucrose preculture, respectively. fully hydrated axes did not survive exposure to liquid nitrogen. highest seedling recovery (93-100%) for untreated axes was observed at 0.26 to 0.15 mg h2o/mg ... | 2009 | 14566388 |
| chemical examination of citrus sinensis flavedo variety pineapple. | phytochemical examination of citrus sinensis flavedo var. pineapple resulted in the isolation of six compounds characterized as tetracosane, ethyl pentacosanoate, tetratriacontanoic acid, tangertin, beta-sitosteryl-beta-d-glucoside and 3,5,4'-trihydroxy-7,3'-dimethoxy flavanone 3-o-beta-glucoside. of these 3,5,4'-trihydroxy-7,3'-dimethoxy flavanone 3-o-beta-glucoside is a hitherto unreported compound. | 2009 | 20376223 |
| the antioxidant activity and polyphenolic contents of different plant seeds extracts. | different plant seeds extracts of citrus sinensis, hordeum sativum, triticum sativum, canna indica, citrullus vulgaris and capsicum annuum were evaluated for their antioxidant activity by the following methods: 2,2-diphenyl-1-pycril-hydrazyl (dpph) radical scavenging, reducing power, rbcs hemolysis and linoleic acid oxidation, a long with the determination of total phenolic and flavonoids contents. all the methanolic extracts showed high antioxidant activity and have high contents of phenolic an ... | 2009 | 19943462 |
| preliminary in vitro antisickilng properties of crude juice extracts of persia americana, citrus sinensis, carica papaya and ciklavit®. | the antisickling properties of crude juice extracts of the edible portions of three commonly consumed tropical fruits namely persia americana, citrus sinensis, and carica papaya were investigated in vitro alongside a new drug preparation called ciklavit® that has antisickling activity. four different solvent extracts of the crude juice of each fruit including aqueous, acidic, alkaline and alcoholic extracts were prepared and their antisickling effects on sickle cell trait (hbas) and sickle cell ... | 2009 | 21304622 |
| evaluation of larvicidal and nymphicidal potential of plant extracts against anopheles subpictus grassi, culex tritaeniorhynchus giles and aphis gossypii glover. | the acetone, chloroform, ethyl acetate, hexane and methanol extracts of peel and leaf extracts of citrus sinensis, ocimum canum, ocimum sanctum and rhinacanthus nasutus were tested against fourth instar larvae of malaria vector, anopheles subpictus grassi, japanese encephalitis vector, culex tritaeniorhynchus giles (diptera: culicidae) and feeding deterrence to nymphs of cotton pest, aphis gossypii glover (homoptera: aphididae). the larval and nymph mortality were observed after 24 h of exposure ... | 2009 | 19050919 |
| citrus biotechnology: achievements, limitations and future directions. | citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improveme ... | 2009 | 23572908 |
| molecular and functional characterization of a novel chromoplast-specific lycopene beta-cyclase from citrus and its relation to lycopene accumulation. | carotenoids are the main pigments responsible of the colouration of citrus fruits. the beta-cyclization of lycopene, catalysed by the lycopene beta-cyclases (beta-lcy), seems to be a key regulatory step of the carotenoid pathway. in the present study, two beta-lcys from orange fruits (citrus sinensis), named csbeta-lcy1 and csbeta-lcy2 have been isolated and the activity of the encoded proteins was demonstrated by functional analysis. csbeta-lcy1 was expressed at low levels and remained relative ... | 2009 | 19325166 |
| detection of huanglongbing (citrus greening) disease by nucleic acid spot hybridization. | polymerase chain reaction (pcr) amplification with primers specific to the rdna region successfully amplified the 1160-bp dna fragment from a huanglongbing (hlb)-infected sweet orange sample with mottling symptoms leaves, but not from healthy sweet orange plants. the pcr product of 1160-bp was used as probe labeled with biotin for detection of the hlb pathogen in the nucleic acid spot hybridization (nash) test. it was found that the hlb pathogen could be detected up to 1:100 dilution in hlb-infe ... | 2009 | 19957441 |
| [survey of sharpshooters (hemiptera: cicadellidae) associated with xylella fastidiosa transmission in citrus groves of the north coast of bahia state]. | the causal agent of citrus variegated clorosis, xylella fastidiosa, is transmitted by leafhoppers of the subfamily cicadellinae, whose species vary regionally. the goal of this study was to identify potential vectors of this pathogen in citrus groves of bahia north coast, brazil. the survey was done from march/2002 to february/2003 in three seven- to nine-year-old sweet orange (citrus sinensis, pêra variety) groves located in rio real, ba. fifteen yellow sticky cards (8.5x11.5 cm) were installed ... | 2009 | 20098930 |
| [diversity of hemiptera auchenorrhyncha in citrus, coffee and a fragment of native forest of the state of são paulo]. | the population of hemiptera auchenorrhyncha was studied in sweet citrus groves (citrus sinensis), coffee plantations (coffea arabica) and a semi-deciduous seasonal forest with shrub physiognomy in bebedouro, sp, to evaluate the influence of the natural ecosystem on the species composition of the agroecosystems. monitoring was carried out by using yellow stick cards, which were replaced every 15 days and all auchenorrhyncha collected were counted and identified. seven families, 11 subfamilies and ... | 2009 | 20098931 |
| host-pathogen-biocontrol agent interaction as affected by sequential application of na2co3 and cacl2. | among the alternatives to synthetic postharvest fungicides encouraging results have been reported with biocontrol agents, and on citrus fruits, their efficacy was improved when co-applied with gras compounds or with physical means. still, the reason for this increased efficacy has not been explained and therefore a study was performed using orange fruit (citrus sinensis osbec. cv 'washington navel') as host, p. digitatum as the pathogen, a yeast (pichia guiliermondii, isolate 5a) as the biocontr ... | 2009 | 20222553 |
| pharmacological exploration of the sedative mechanism of hesperidin identified as the active principle of citrus sinensis flowers. | the infusion of flowers of several species of citrus genera is used as a sedative to treat insomnia in mexican traditional medicine. the aims of this study were to investigate the sedative effect of different extracts of flowers of citrus sinensis (l.) osbeck (rutaceae) and describe the pharmacological action mechanism of the sedative active compounds of this plant. the methanol and dichloromethane extracts, obtained from the flowers of citrus sinensis (l.) osbeck (rutaceae), showed a dose-depen ... | 2009 | 19219759 |
| characterization of citrus sinensis type 1 mitochondrial alternative oxidase and expression analysis in biotic stress. | the higher plant mitochondrial electron transport chain contains an alternative pathway that ends with the aox (alternative oxidase). the aox proteins are encoded by a small gene family composed of two discrete gene subfamilies. aox1 is present in both monocot and eudicot plants, whereas aox2 is only present in eudicot plants. we isolated a genomic clone from citrus sinensis containing the aox1a gene. the orange aox1a consists of four exons interrupted by three introns and its promoter harbours ... | 2009 | 19257856 |
| monodemethylated polymethoxyflavones from sweet orange (citrus sinensis) peel inhibit growth of human lung cancer cells by apoptosis. | polymethoxyflavones (pmfs) are almost exclusively found in the citrus genus, particularly in the peels of sweet orange (citrus sinensis l. osbeck) and mandarin (c. reticulate blanco). we studied the effects of two major pmfs, namely, nobiletin and 3,5,6,7,8,3',4'-heptamethoxyflavone (hmf), and two major monodemethylated pmfs, namely 5-hydroxy-3,7,8,3',4'-pentamethoxyflavone (5hpmf), and 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5hhmf), on the growth of human lung cancer h1299, h441, and h460 c ... | 2009 | 19065586 |
| in vitro antilisterial effects of citrus oil fractions in combination with organic acids. | the objectives of this study were to screen activity of citrus essential oil fractions (eos) alone and in combination with organic acids against 2 species of listeria. five citrus eos were initially screened by disc diffusion assay for antibacterial activity. cold pressed terpeneless valencia orange oil (cp terpeneless oil) had the strongest bacteriostatic (mic) and bactericidal (mbc) properties at 0.55% and 1.67%, respectively. four organic acids were tested for effectiveness against listeria. ... | 2009 | 19323760 |
| host range and genetic relatedness of colletotrichum acutatum isolates from fruit crops and leatherleaf fern in florida. | isolates of colletotrichum acutatum were collected from anthracnose-affected strawberry, leatherleaf fern, and key lime; ripe-rot-affected blueberry; and postbloom fruit drop (pfd)-affected sweet orange in florida. additional isolates from ripe-rot-affected blueberry were collected from georgia and north carolina and from anthracnose-affected leatherleaf fern in costa rica. pathogenicity tests on blueberry and strawberry fruit; foliage of key lime, leatherleaf fern, and strawberry; and citrus fl ... | 2009 | 19351258 |
| radical scavenging activity of orange and tangerine varieties cultivated in brazil. | four citrus fruit varieties cultivated in brazil (two kinds of sweet orange and two kinds of tangerine) were analyzed for physicochemical characteristics contents of total phenolics, total carotenoids and ascorbic acid, and antioxidant activities. the antioxidant activities of aqueous, methanolic, and acetone extracts of the citrus fruit juices were assessed on the basis of their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (dpph( )). the cravo tangerine has the highest content of citric ac ... | 2009 | 19381992 |
| citrus essential oils and four enantiomeric pinenes against culex pipiens (diptera: culicidae). | the aim of this study was to evaluate the toxicity of pinenes (enantiomers of alpha- and beta-) and essential oils from greek plants of the rutaceae family against the mosquito larvae of culex pipiens (diptera: culicidae). essential oils were isolated by hydrodistillation from fruit peel of orange (citrus sinensis l.), lemon (citrus limon l.), and bitter orange (citrus aurantium l.). the chemical composition was determined by gas chromatography/mass spectrometry (gc/ms) analysis. citrus essentia ... | 2009 | 19424721 |
| construction and characterization of two citrus bac libraries and identification of clones containing the phytoene synthase gene. | two deep-coverage bacterial artificial chromosome (bac) libraries of citrus sinensis (l.) osbeck 'cara cara' navel orange and citrus reticulata (l.) blanco 'egan no. 1' ponkan mandarin, which belong to the two most important species of the citrus genus, have been constructed and characterized to facilitate gene cloning and to analyze variety-specific genome composition. the c. sinensis bac library consists of 36 000 clones with negligible false-positive clones and an estimated average insert siz ... | 2009 | 19448728 |
| quantitative distribution of 'candidatus liberibacter asiaticus' in citrus plants with citrus huanglongbing. | citrus huanglongbing (hlb), or greening disease, is strongly associated with any of three nonculturable gram-negative bacteria belonging to 'candidatus liberibacter spp.' 'ca. liberibacter spp.' are transmitted by citrus psyllids to all commercial cultivars of citrus. the diseases can be lethal to citrus and have recently become widespread in both são paulo, brazil, and florida, united states, the locations of the largest citrus industries in the world. asiatic hlb, the form of the disease found ... | 2009 | 19159305 |
| enantiomeric composition of (3r)-(-)- and (3s)-(+)-linalool in various essential oils of indian origin by enantioselective capillary gas chromatography-flame ionization and mass spectrometry detection methods. | enantiomeric ratios of linalool have been determined in various authentic essential oils of indian origin using 10% heptakis(2,3-di-o-methyl-6-o-tert-butyldimethylsilyl)-beta-cyclodextrin as a chiral stationary phase. a complete enantiomeric excess (ee) for (3s)-(+)-linalool was characteristic of lippia alba and cinnamomum tamala leaf oils while less than 90% excess was noticed in zanthoxylum armatum leaf, zingiber roseum root/rhizome and citrus sinensis leaf oils. on the contrary, an enantiomer ... | 2009 | 19476006 |
| intraplantar injection of bergamot essential oil into the mouse hindpaw: effects on capsaicin-induced nociceptive behaviors. | despite the increasing use of aromatherapy oils, there have not been many studies exploring the biological activities of bergamot (citrus bergamia, risso) essential oil (beo). recently, we have investigated the effects of beo injected into the plantar surface of the hindpaw in the capsaicin test in mice. the intraplantar injection of capsaicin produced an intense and short-lived licking/biting response toward the injected hindpaw. the capsaicin-induced nociceptive response was reduced significan ... | 2009 | 19607974 |
| essential oils and anxiolytic aromatherapy. | a number of essential oils are currently in use as aromatherapy agents to relieve anxiety, stress, and depression. popular anxiolytic oils include lavender (lavandula angustifolia), rose (rosa damascena), orange (citrus sinensis), bergamot (citrus aurantium), lemon (citrus limon), sandalwood (santalum album), clary sage (salvia sclarea), roman chamomile (anthemis nobilis), and rose-scented geranium (pelargonium spp.). this review discusses the chemical constituents and cns effects of these aroma ... | 2009 | 19831048 |
| comparative transcripts profiling reveals new insight into molecular processes regulating lycopene accumulation in a sweet orange (citrus sinensis) red-flesh mutant. | interest in lycopene metabolism and regulation is growing rapidly because accumulative studies have suggested an important role for lycopene in human health promotion. however, little is known about the molecular processes regulating lycopene accumulation in fruits other than tomato so far. | 2009 | 19922663 |
| in vitro inhibition of vancomycin-susceptible and vancomycin-resistant enterococcus faecium and e. faecalis in the presence of citrus essential oils. | glycopeptide-resistant enterococcus (gre) is an important healthcare-acquired infection (hcai) which costs the healthcare service many millions of pounds worldwide. in this study, lemon (citrus limon), sweet orange (citrus sinensis) or bergamot (citrus bergamia) essential oils (eo) and their vapours, alone and in combination, are tested for their antimicrobial activity against vancomycin-resistant and vancomycin-sensitive strains of e. faecium and e. faecalis. a blend of 1:1 (v/v) orange and ber ... | 2009 | 20095125 |
| transmission of different isolates of spiroplasma citri to carrot and citrus by circulifer tenellus (hemiptera: cicadellidae). | carrot purple leaf disease was first reported in 2006 in the state of washington and was associated with spiroplasma citri. the disease also was reported in california in 2008. the objectives of this work were to fulfill koch's postulates and to determine 1) whether the beet leafhopper, circulifer tenellus (baker) (hemiptera: cicadellidae), transmits carrot [daucus carota l. subsp. sativus (hoffm.) arcang] isolates of s. citri; and 2) whether carrot and citrus [citrus sinensis (l.) osb.]-derived ... | 2009 | 19736751 |
| diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy. | endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. in the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (its) rrna. plants sampled from four different sites in the state of são paulo, brazil exhibited different levels of ... | 2009 | 19798655 |
| campylobacter and arcobacter species sensitivity to commercial orange oil fractions. | seven orange oil fractions were screened for their ability to inhibit the growth of selected campylobacter and arcobacter spp. using the standard agar-disk diffusion assay. cold pressed (cp) terpeneless valencia orange oil was found to be the most inhibitory to both campylobacter jejuni and campylobacter coli, exhibiting maximum zones of inhibition up to 80+/-0.0 mm. five-fold concentrated valencia oil and distilled d-limonene resulted in campylobacter inhibition zones ranging from 11.0+/-1.4 to ... | 2009 | 19070381 |
| a novel hybridization approach for detection of citrus viroids. | citrus plants are natural hosts of several viroid species all belonging to the family pospiviroidae. previous attempts to detect viroids from field-grown species and cultivars yielded erratic results unless analyses were performed using etrog citron a secondary bio-amplification host. to overcome the use of etrog citron a number of rt-pcr approaches have been proposed with different degrees of success. here we report the suitability of an easy to handle northern hybridization protocol for viroid ... | 2009 | 19162174 |
| the mechanism of action of a citrus oil blend against enterococcus faecium and enterococcus faecalis. | the aim was to explore the mechanisms by which a blend of orange (citrus sinensis) : bergamot (citrus bergamia) (1 : 1 v/v) eo (essential oil) (2% v/v) and its vapour (15 mg l(-1) air) brings about its antimicrobial effect against enterococcus faecium and enterococcus faecalis. | 2009 | 19187138 |
| effect of citrus paradisi extract and juice on arterial pressure both in vitro and in vivo. | citrus paradisi (grapefruit) consumption is considered as beneficial and it is popularly used for the treatment of a vast array of diseases, including hypertension. in the present study, the coronary vasodilator and hypotensive effects of citrus paradisi peel extract were assessed in the langendorff isolated and perfused heart model and in the heart and lung dog preparation. in both models, citrus paradisi peel extract decreased coronary vascular resistance and mean arterial pressure when compar ... | 2009 | 19153985 |
| high-efficiency agrobacterium-mediated transformation of citrus via sonication and vacuum infiltration. | an improved method for the agrobacterium infiltration of epicotyl segments of 'pineapple' sweet orange [citrus sinensis (l.) osbeck] and 'swingle' citrumelo [citrus paradisi macf. x poncirus trifoliata (l.) raf.] was developed in order to increase transformation frequency. sonication-assisted agrobacterium-mediated transformation (saat), vacuum infiltration, and a combination of the two procedures were compared with conventional agrobacterium-mediated inoculation method ('dipping' method). it wa ... | 2009 | 19048258 |
| nuclear magnetic resonance characterization of metabolite disorder in orange trees caused by citrus sudden death disease. | citrus sudden death (csd) is a new disease of sweet orange and mandarin trees grafted on rangpur lime and citrus volkameriana rootstocks. it was first seen in brazil in 1999, and has since been detected in more than four million trees. the csd causal agent is unknown and the current hypothesis involves a virus similar to citrus tristeza virus or a new virus named citrus sudden death-associated virus. csd symptoms include generalized foliar discoloration, defoliation and root death, and, in most ... | 2009 | 19161352 |
| ciprofloxacin-sensitive and ciprofloxacin-resistant campylobacter jejuni are equally susceptible to natural orange oil-based antimicrobials. | a total of 10 ciprofloxacin-sensitive (ciprofloxacin minimum inhibitory concentration, mic < 0.5 micro g/ml) and 10 ciprofloxacin-resistant (mic 16 to 32 micro g/ml) presumptive c. jejuni were further characterized and evaluated for their inhibition by natural orange oil fractions. partial species identification was performed by using a hippuricase gene-based polymerase chain reaction (pcr) assay. one of the isolates appeared to be atypical and failed to hydrolyze hippurate. of the ciprofloxacin ... | 2009 | 20183064 |
| response of sweet orange (citrus sinensis) to 'candidatus liberibacter asiaticus' infection: microscopy and microarray analyses. | citrus greening or huanglongbing (hlb) is a devastating disease of citrus. hlb is associated with the phloem-limited fastidious prokaryotic alpha-proteobacterium 'candidatus liberibacter spp.' in this report, we used sweet orange (citrus sinensis) leaf tissue infected with 'ca. liberibacter asiaticus' and compared this with healthy controls. investigation of the host response was examined with citrus microarray hybridization based on 33,879 expressed sequence tag sequences from several citrus sp ... | 2009 | 19055434 |
| effect of surface roughness on retention and removal of escherichia coli o157:h7 on surfaces of selected fruits. | this study was undertaken to evaluate the effect of surface roughness on the attachment and removal of escherichia coli o157:h7 on selected fruit and metal surfaces. a new method to determine surface roughness was developed using confocal laser scanning microscopy (clsm). a series of 2-d layered images were taken by clsm optical slicing of the surfaces of golden delicious apples, navel oranges, avocadoes, and cantaloupes. the average roughness (r(a)) of the fruit surfaces was assessed by reconst ... | 2009 | 19200095 |
| discrimination between mild and severe citrus tristeza virus isolates with a rapid and highly specific real-time reverse transcription-polymerase chain reaction method using taqman lna probes. | severe isolates of citrus tristeza virus (ctv) inducing seedling yellows (sy) and/or stem pitting (sp) in grapefruit or sweet orange are a major threat for the citrus industry worldwide. identification of these ctv variants was achieved by quantitative real-time reverse transcription-polymerase chain reaction (rt-pcr) using a general primer set and three taqman locked nucleic acids (lna) probes targeting sequences characteristic of severe, mild (non-sy, non-sp), and t36-like isolates. successful ... | 2009 | 19203284 |
| isolation of xanthyletin, an inhibitor of ants' symbiotic fungus, by high-speed counter-current chromatography. | xanthyletin, an inhibitor of symbiotic fungus (leucoagaricus gongylophorus) of leaf-cutting ant (atta sexdens rubropilosa), as well as suberosin, seselin and xanthoxyletin were isolated from citrus sinensis grafted on citrus limonia. a two-phase solvent system composed of hexane/ethanol/acetonitrile/water (10:8:1:1, v/v) was used for the high-speed counter-current chromatographic isolation of xanthyletin with high yield and over 99% purity as determined by liquid and gas chromatography with mass ... | 2009 | 19296958 |
| sequencing and computational analysis of complete genome sequences of citrus yellow mosaic badna virus from acid lime and pummelo. | citrus yellow mosaic badna virus (cmbv), a member of the family caulimoviridae, genus badnavirus, is the causative agent of citrus mosaic disease in india. although the virus has been detected in several citrus species, only two full-length genomes, one each from sweet orange and rangpur lime, are available in publicly accessible databases. in order to obtain a better understanding of the genetic variability of the virus in other citrus mosaic-affected citrus species, we performed the cloning an ... | 2009 | 19444599 |
| pathotypes and genetic relationship of worldwide collections of elsinoë spp. causing scab diseases of citrus. | two scab diseases are recognized currently on citrus: citrus scab, caused by elsinoë fawcettii, and sweet orange scab, caused by e. australis. because the two species cannot be reliably distinguished by morphological or cultural characteristics, host range and molecular methods must be used to identify isolates. four pathotypes of e. fawcettii and two of e. australis have been described to date based on host range. the host specificity and genetic relationships among 76 isolates from argentina, ... | 2009 | 19453232 |
| [biology of the citrus blackfloy, aleurocanthus woglumi ashby (hemiptera: aleyrodidae), in three host plants]. | the citrus blackfloy, aleurocanthus woglumi ashby, was detected in brazil in 2001. the aim of this research was to evaluate the biology, biometry and host preference of a. woglumi in sweet orange, acid lime tahiti and mango. experiments were set in laboratory conditions with insects collected in rangpur lime plants in manaus, state of amazonas, from january to june of 2006. the following parameters were evaluated: number of spirals (ovopositions) and eggs per plant, number of eggs by spiral per ... | 2009 | 19488516 |
| exposure to pesticides residues from consumption of italian blood oranges. | this paper reports the results of a 5-year study to evaluate pesticide levels, derived from orchard activities, on italy's most common orange cultivar (citrus sinensis, l. osbeck, cv. tarocco). using a bayesian approach, the study allowed both the qualitative (number) and quantitative distributions (amount) of pesticides to be determined with its own probability value. multi-residue analyses of 460 samples highlighted the presence of ethyl and methyl chlorpyrifos, dicofol, etofenprox, fenazaquin ... | 2009 | 19680977 |
| characterization of gibberellin 20-oxidases in the citrus hybrid carrizo citrange. | ccga20ox1 is a gene encoding a ga 20-oxidase, a gibberellin (ga) biosynthetic enzyme, previously isolated from the citrus hybrid carrizo citrange (citrus sinensis (l.) osbeck x poncirus trifoliata (l.) raf.). southern blot analysis of genomic dna of carrizo citrange with ccga20ox1 suggested the presence in the hybrid of another gene encoding another ga 20-oxidase. a cdna clone from this new gene (ccga20ox2) was isolated using rna from the other parent c. sinensis. ccga20ox2 encoded a protein of ... | 2009 | 19203975 |
| segregation of distinct variants from citrus tristeza virus isolate sy568 using aphid transmission. | a well-studied severe isolate of citrus tristeza virus (ctv) known as sy568 has previously been shown to contain multiple variants of the virus which differ in their genetic and biological characters. aphid transmission was used in an attempt to segregate some of these variants for further characterization. resulting infections gave symptoms which varied from asymptomatic to more severe than the inoculum source. rnase protection assays (rpas) were used to compare nine regions of the ctv genome a ... | 2009 | 19740030 |
| a comparison of citrus blossom volatiles. | the objective of this study was to identify the major volatiles and their relative concentrations in intact grapefruit, sweet orange, sour orange, mandarin, lemon, lime and pummelo blossoms. volatiles from freshly picked blossoms were collected and concentrated using static headspace solid-phase microextraction and then separated and identified using gc-ms. seventy volatiles were detected, 66 identified, of which 29 were identified for the first time in citrus blossoms. major volatiles consisted ... | 2009 | 19747702 |
| roles of olfactory cues, visual cues, and mating status in orientation of diaphorina citri kuwayama (hemiptera: psyllidae) to four different host plants. | diaphorina citri kuwayama (hemiptera: psyllidae) is an important worldwide pest of citrus that vectors bacteria (candidatus liberibacter spp.) responsible for huanglongbing (citrus greening disease). we examined the behavioral responses of mated and unmated d. citri of both sexes to odors from host plants in a y-tube olfactometer, with and without visual cues. the host plants tested were 'duncan' grapefruit (citrus paradisi macfayden), sour orange (citrus aurantium l.), navel orange (c. sinensis ... | 2009 | 19791618 |
| effect of genotype and environment on citrus juice carotenoid content. | a selection of orange and mandarin varieties belonging to the same citrus accession and cultivated in mediterranean (corsica), subtropical (new caledonia), and tropical areas (principally tahiti) were studied to assess the effect of genotype and environmental conditions on citrus juice carotenoid content. juices from three sweet orange cultivars, that is, pera, sanguinelli, and valencia ( citrus sinensis (l.) osbeck), and two mandarin species ( citrus deliciosa ten and citrus clementina hort. ex ... | 2009 | 19807162 |
| functional characterization of citrus psy gene in hongkong kumquat (fortunella hindsii swingle). | citrus, rich in carotenoids, is the most important fruit crop based on the total annual production. in the carotenoid biosynthesis pathway, phytoene synthase (psy, ec 2.5.1.32) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate (ggpp) to phytoene and has been shown to be a rate-limiting enzyme for the synthesis of carotenoids. in this study, we investigated catalytic activity of cspsy from cara cara navel orange (citrus sinensis osbeck) by heterologous expression in esch ... | 2009 | 19813015 |
| targeting excessive free radicals with peels and juices of citrus fruits: grapefruit, lemon, lime and orange. | a comparative study between the antioxidant properties of peel (flavedo and albedo) and juice of some commercially grown citrus fruit (rutaceae), grapefruit (citrus paradisi), lemon (citrus limon), lime (citrusxaurantiifolia) and sweet orange (citrus sinensis) was performed. different in vitro assays were applied to the volatile and polar fractions of peels and to crude and polar fraction of juices: 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging capacity, reducing power and inhibition o ... | 2010 | 19770018 |
| liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates. | a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ... | 2010 | 3417817 |
| distribution and biological role of the oligopeptide-binding protein (oppa) in xanthomonas species. | in this study we investigated the prevalence of the oppa gene, encoding the oligopeptide binding protein (oppa) of the major bacterial oligopeptide uptake system (opp), in different species of the genus xanthomonas. the oppa gene was detected in two xanthomonas axonopodis strains among eight tested xanthomonas species. the generation of an isogenic oppa-knockout derivative of the xac 306 strain, showed that the oppa protein neither plays a relevant role in oligopeptide uptake nor contributes to ... | 2010 | 21637492 |
| effects of pymetrozine, an antifeedant of hemiptera, on asian citrus psyllid, diaphorina citri, feeding behavior, survival and transmission of candidatus liberibacter asiaticus. | vector-borne plant disease management can be enhanced by deployment of antifeedants in addition to the use of broad-spectrum neurotoxic insecticides. the effects of pymetrozine on asian citrus psyllid (acp), diaphorina citri kuwayama, feeding behaviour, survival and transmission of candidatus liberibacter asiaticus (las), the presumed causal pathogen of huanglongbing, were investigated. | 2010 | 20960465 |
| transformation of sweet orange [citrus sinensis (l.) osbeck] with ptha-nls for acquiring resistance to citrus canker disease. | the cooh terminal of ptha encoding three nuclear localizing signals (nls) was amplified by polymerase chain reaction (pcr) from the plasmid of xanthomonas axonopodis pv. citri, the pathogen of citrus canker disease. then the sense and antisense strands of the nls were cloned into pbi121 vector. ptha-nls driven by the camv35 s promoter was transferred into sweet orange via agrobacterium -mediated transformation. successful integration was confirmed by pcr and southern blotting, and 12 sense-nls ( ... | 2010 | 20972821 |
| effective sampling range of a synthetic protein-based attractant for ceratitis capitata (diptera: tephritidae). | studies were conducted in honduras to determine effective sampling range of a female-targeted protein-based synthetic attractant for the mediterranean fruit fly, ceratitis capitata (wiedemann) (diptera: tephritidae). multilure traps were baited with ammonium acetate, putrescine, and trimethylamine lures (three-component attractant) and sampled over eight consecutive weeks. field design consisted of 38 traps (over 0.5 ha) placed in a combination of standard and high-density grids to facilitate ge ... | 2010 | 21061993 |
| antiplasmodial activity of botanical extracts against plasmodium falciparum. | the absence of a vaccine and the rampant resistance to almost all antimalarial drugs have accentuated the urgent need for new antimalarial drugs and drug targets for both prophylaxis and chemotherapy. the aim of the study was to discover effective plant extracts against plasmodium falciparum. in the present study, the hexane, chloroform, ethyl acetate, acetone, and methanol extracts of citrus sinensis (peel), leucas aspera, ocimum sanctum, phyllanthus acidus (leaf), terminalia chebula (seed) wer ... | 2010 | 21079994 |
| resistance to citrus psorosis virus in transgenic sweet orange plants is triggered by coat protein-rna silencing. | the lack of naturally occurring resistance to citrus psorosis virus (cpsv) has demanded exploitation of a transgenic approach for the development of cpsv-resistant sweet orange plants. transgenic sweet orange plants producing intron-hairpin rna transcripts (ihprna) corresponding to viral cp, 54k or 24k genes were generated and analyzed at the molecular and phenotypic levels. two independent cpsv challenge assays demonstrated that expression of ihprna derived from the cp gene (ihpcp) provided a h ... | 2010 | 21084056 |
| rapid simultaneous determination of amines and organic acids in citrus using high-performance liquid chromatography. | rapid analytical method for the simultaneous separation and determination of amines and organic acids is a vital interest for quality control of citrus and their products. in the present study, a simultaneous high performance liquid chromatography (hplc) method for the rapid separation of three amines and two organic acids was developed. chromatographic separation of compounds was achieved using xbridge c(18) column at ambient temperature, with an isocratic mobile phase of 3mm phosphoric acid at ... | 2010 | 21147342 |
| insecticidal activity against bemisia tabaci biotype b of peel essential oil of citrus sinensis var. pear and citrus aurantium cultivated in northeast brazil. | the fumigant action of peel essential oils of citrus sinensis var. pear (pear orange = po) and c. aurantium (bitter orange = bo) from the northeast of brazil were evaluated against bemisia tabaci biotype b and compared with eugenol as a positive control. the oil concentration in the po at 8.5 microl/l of air caused 97% mortality, while the oil concentration of bo at 9.5 microl/l of air caused 99% mortality. however, the lc50 estimates for both oils (lc50 = 3.80 microl/l of air for po and lc50 = ... | 2010 | 21213990 |
| distribution of aroma volatile compounds in tangerine hybrids and proposed inheritance. | with the desirable combination of sugars and acids, volatile compounds contribute to the essential organoleptic attributes of citrus. this study evaluated the aroma volatiles of 20 tangerine hybrids of the university of florida breeding program. volatiles were sampled from hand-squeezed juice by headspace solid-phase microextraction (spme), and analyzed by gas chromatography-mass spectrometry. principal component analysis (pca) and cluster analysis (ca) were used to find similarities among sampl ... | 2010 | 21218478 |
| azteca barbifex forel (hymenoptera: formicidae): potential pest of citrus crops in eastern amazon. | damage caused by the ant azteca barbifex (forel) was identified in orange trees (citrus sinensis), in capitão poço county, guamá microregion, pará state. the damage caused by the scraping of stems and branches lead to reduction in yield with subsequent death of the plant. these characteristics indicate a. barbifex as a potential pest of citrus crops in the eastern region of amazon. | 2010 | 21271082 |
| acaricidal activity against tetranychus urticae and chemical composition of peel essential oils of three citrus species cultivated in ne brazil. | the repellency and fumigant toxicities of the peel essential oils of citrus sinensis var. pêra (lp), c. sinensis var. mimo (lm), and c. aurantium (ll) cultivated in northeast brazil were evaluated against tetranychus urticae. analysis of the oils by gc and gc/ms led to the identification of twenty-eight components, which represented 99.9%, 99.7% and 99.3% of the total constituents of the lp, lm and ll oils, respectively. limonene was the main component found in all three oils. other main compone ... | 2010 | 20420330 |
| stereoselective conjugation, transport and bioactivity of s- and r-hesperetin enantiomers in vitro. | the flavanone hesperetin ((+/-)-4'-methoxy-3',5,7-trihydroxyflavanone) is the aglycone of hesperidin, which is the major flavonoid present in sweet oranges. hesperetin contains a chiral c-atom and so can exist as an s- and r-enantiomer, however, in nature 2s-hesperidin and its s-hesperetin aglycone are predominant. the present study reports a chiral hplc method to separate s- and r-hesperetin on an analytical and semipreparative scale. this allowed characterization of the stereoselective differe ... | 2010 | 20441166 |
| microbial inhibitory and radical scavenging activities of cold-pressed terpeneless valencia orange (citrus sinensis) oil in different dispersing agents. | due to their low solubility in water, oil-based bioactive compounds require dispersion in a surface-active agent or appropriate solvents to ensure maximum contact with microorganisms. these combinations, however, may change their physical and/or chemical characteristics and consequently alter the desired functionality. the objective of this study was to determine the impact of selected dispersing agents, ethanol, dimethyl sulfoxide (dmso), and tween-80, on cold-pressed terpeneless (cpt) valencia ... | 2010 | 20355124 |
| chemical profile, antifungal, antiaflatoxigenic and antioxidant activity of citrus maxima burm. and citrus sinensis (l.) osbeck essential oils and their cyclic monoterpene, dl-limonene. | the study deals with antifungal, antiaflatoxigenic and antioxidant activity of citrus maxima and citrus sinensis essential oils (eos) and their phytochemical composition. the eos were obtained by hydrodistillation and their chemical profile was determined through gc and gc-ms analysis. both the eos and their 1:1 combination showed broad fungitoxic spectrum against different food contaminating moulds. the eos and their combination completely inhibited aflatoxin b(1) (afb(1)) production at 500 ppm ... | 2010 | 20385194 |
| a comparison of volatile components of setomi with its parent cultivars. | the citrus fruit setomi is a hybrid of yoshiura ponkan (citrus reticulate blanco) and kiyomi (citrus unshiu mrcov. x citrus sinensis osbeck). the essential oils from the peel of setomi and its parent cultivars were obtained by a simultaneous distillation extraction technique. comparing the essential oils of setomi and its parent cultivars, it was found that the oil in the peel of setomi consisted of characteristic aroma components from each parent cultivar. the principal component analysis of da ... | 2010 | 20208376 |
| anxiolytic-like effect of sweet orange aroma in wistar rats. | aromatherapy is the use of essential oils as an alternative treatment for medical purposes. despite the lack of sufficient scientific proof, it is considered a holistic complementary therapy employed to enhance comfort and decrease distress. citrus fragrances have been particularly used by aromatherapists for the treatment of anxiety symptoms. based on this claim, the present study investigated the effects of citrus sinensis (sweet orange) essential oil on wistar, male rats evaluated in the elev ... | 2010 | 20211673 |
| effect of ploidy increase on transgene expression: example from citrus diploid cybrid and allotetraploid somatic hybrid expressing the egfp gene. | polyploidization is an important speciation mechanism for all eukaryotes, and it has profound impacts on biodiversity dynamics and ecosystem functioning. green fluorescent protein (gfp) has been used as an effective marker to visually screen somatic hybrids at an early stage in protoplast fusion. we have previously reported that the intensity of gfp fluorescence of regenerated embryoids was also an early indicator of ploidy level. however, little is known concerning the effects of ploidy increas ... | 2010 | 20734092 |
| shedding light on the role of photosynthesis in pathogen colonization and host defense. | the role of photosynthesis in plant defense is a fundamental question awaiting further molecular and physiological elucidation. to this end we investigated host responses to infection with the bacterial pathogen xanthomonas axonopodis pv. citri, the pathogen responsible for citrus canker. this pathogen encodes a plant-like natriuretic peptide (xacpnp) that is expressed specifically during the infection process and prevents deterioration of the physiological condition of the infected tissue. prot ... | 2010 | 20798833 |
| involvement of inducible nitric oxide synthase and cyclooxygenase-2 in the anti-inflammatory effects of a red orange extract in human chondrocytes. | in the present study, a complex of compounds (red orange complex, roc), obtained from three red orange varieties (citrus sinensis varieties: moro, tarocco and sanguinello), containing cyanidin glycosides, hydroxycinnamic acids, flavanone glycosides and ascorbic acid, was screened to discover new lead compounds in the suppression of the production of key molecules released during inflammatory events in interleukin-1beta (il-beta) stimulated human primary chondrocytes. the expression of inducible ... | 2010 | 20812134 |
| evaluation of in vitro activity of essential oils against trypanosoma brucei brucei and trypanosoma evansi. | essential oils (eos) from cymbopogon citratus (cc), eucalyptus citriodora (ec), eucalyptus camaldulensis (ed), and citrus sinensis (cs) were obtained by hydrodistillation process. the eos were evaluated in vitro for activity against trypanosoma brucei brucei (tbb) and trypanosoma evansi (t. evansi). the eos were found to possess antitrypanosomal activity in vitro in a dose-dependent pattern in a short period of time. the drop in number of parasite over time was achieved doses of 0.4 g/ml, 0.2 g/ ... | 2010 | 20700425 |
| an attempt of postharvest orange fruit rot control using essential oils from mediterranean plants. | twelve essential oils from mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: botrytis cinerea, penicillium italicum, phytophthora citrophthora, and rhizopus stolonifer. essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis, and thymus vulgaris (family lamiaceae), verbena officinalis (family ... | 2010 | 20874226 |
| citrus fruit extracts reduce advanced glycation end products (ages)- and h(2)o(2)-induced oxidative stress in human adipocytes. | diabetes is a reactive oxygen species (ros)-mediated pathology, with a worldwide prevalence estimated to double by 2030. a major effort has been launched to find therapeutic means to improve health conditions of diabetic patients. recent data show that supplemental natural antioxidants represent a potential strategy as adjunct therapy. despite the major role of adipocytes in the etiology of diabetes, little is known about the effect of natural antioxidants on adipocyte response to oxidative stre ... | 2010 | 20882960 |
| blood orange juice inhibits fat accumulation in mice. | to analyze the effect of the juice obtained from two varieties of sweet orange (citrus sinensis l. osbeck), moro (a blood orange) and navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (hfd). | 2010 | 20029381 |
| antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine. | red oranges are an important component of the so-called mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. the purpose of this study was to evaluate the antiinflammatory activity of a red orange (citrus sinensis varieties: moro, tarocco, sanguinello) complex (roc), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids a ... | 2010 | 19655299 |
| effect of chlorine dioxide gas on salmonella enterica inoculated on navel orange surfaces and its impact on the quality attributes of treated oranges. | microorganisms, including pathogens of public health significance, have been shown to contaminate orange juice during the mechanical extraction of juice. the problem gets exacerbated when washed oranges have high initial microbial load, due to an insufficient postharvest treatment. the objective of this study was to investigate the reduction of salmonella enterica on orange surfaces using clo₂ gas treatments to achieve a 5 log reduction, consistent with the recommendations of the u.s. department ... | 2010 | 20932090 |
| chemical characterization of orange juice from trees infected with citrus greening (huanglongbing). | the effects due to candidatus liberibacter infection, commonly called citrus greening or huanglongbing (hlb), on volatile and nonvolatile components of orange juices, oj, were examined using gc-ms and high-performance liquid chromatography (hplc). hlb symptomatic, asymptomatic, and control "hamlin" and "valencia" oranges were harvested from december to may during the 2007 to 2008 harvest season. brix/acid levels in control and asymptomatic juices were similar but symptomatic juices were as much ... | 2010 | 20492226 |
| different roles of functional residues in the hydrophobic binding site of two sweet orange tau glutathione s-transferases. | glutathione s-transferases (gsts) catalyze the conjugation of glutathione to hydrophobic compounds, contributing to the metabolism of toxic chemicals. in this study, we show that two naturally occurring tau gsts (gstus) exhibit distinctive kinetic parameters towards 1-chloro-2,4-dinitrobenzene (cdnb), although they differ only in three amino acids (arg89, glu117 and ile172 in gstu1 are replaced by pro89, lys117 and val172 in gstu2). in order to understand the effects of the single mismatched res ... | 2010 | 19954490 |
| the effects of host, geographic origin, and gender on the thermal requirements of diaphorina citri (hemiptera: psyllidae). | diaphorina citri kuwayama (hemiptera: psyllidae) is the vector of the bacteria that causes citrus greening and is considered one of the world's most important citrus diseases. we examined how host, geographic region, and gender affect the thermal requirements of d. citri. the insects were reared in climatic chambers at constant temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees c, 70 +/- 10% rh, and a 14 h photophase. host plants for d. citri included orange (citrus sinensis [rutaceae] ... | 2010 | 20388302 |
| effect of liberibacter infection (huanglongbing or "greening" disease) of citrus on orange juice flavor quality by sensory evaluation. | some anecdotal reports suggest that infection of citrus trees with candidatus liberibacter asiaticus (las), the suspected causal agent of huanglongbing (hlb) disease, imparts off flavor to orange juice. it is of interest to the industry to know how las infection affects juice quality with respect to cultivar, maturity, or processing method. hamlin, midsweet, and valencia oranges were harvested over 2 y from trees that tested negative (las-) or positive (las+) for las from different groves and in ... | 2010 | 20546425 |
| early events of citrus greening (huanglongbing) disease development at the ultrastructural level. | citrus greening (huanglongbing [hlb]) is one of the most destructive diseases of citrus worldwide. the causal agent of hlb in florida is thought to be 'candidatus liberibacter asiaticus'. understanding of the early events in hlb infection is critical for the development of effective measures to control the disease. in this work, we conducted cytopathological studies by following the development of the disease in citrus trees graft inoculated with 'ca. l. asiaticus'-containing material under gree ... | 2010 | 20701493 |
| elsinoë fawcettii and elsinoë australis: the fungal pathogens causing citrus scab. | elsinoë fawcettii and e. australis are important pathogens of citrus. both species are known to produce red or orange pigments, called elsinochrome. elsinochrome is a nonhost-selective phytotoxin and is required for full fungal virulence and lesion formation. this article discusses the taxonomy, epidemiology, genetics and pathology of the pathogens. it also provides a perspective on the cellular toxicity, biosynthetic regulation and pathological role of elsinochrome phytotoxin. taxonomy: elsinoë ... | 2010 | 21199563 |
| glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation. | phospholipid hydroperoxide glutathione peroxidase (phgpx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. to study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (citrus sinensis) phgpx (cit-phgpx). all attempts to obtain regenerated plants expressing this enzyme constitutively failed. however, when the enzyme's catalytic activity was abolished by active sit ... | 2010 | 20530511 |
| comparative morpho-anatomical studies of the lesions caused by citrus leprosis virus on sweet orange. | the leprosis disease shows a viral etiology and the citrus leprosis virus is considered its etiologic agent. the disease may show two types of cytopatologic symptom caused by two virus: nuclear (cilv-n) and cytoplasmic (cilv-c) types. the aim of this study was to compare the morpho-anatomical differences in the lesions caused by leprosis virus-cytoplasmic and nuclear types in citrus sinensis (l.) osbeck 'pêra'. leaf and fruit lesions were collected in piracicaba/são paulo (cytoplasmic type) and ... | 2010 | 20563430 |
| pharmacokinetics-based elucidation on disparity in clinical effectiveness between varieties of zhi zhu wan, a traditional chinese medical formula. | zhi zhu wan (zzw) is a classical chinese medical formulation used for the treatment of functional dyspepsia that attributed to spleen-deficiency syndrome. zzw contains atractylodes rhizome and fructus citrus immaturus, the later originates from both citrus aurantium l. (bzzw) and citrus sinensis osbeck (rzzw). the present study is designed to elucidate disparities in the clinical efficacy of two zzw varieties based on the pharmacokinetics of naringenin and hesperetin. mehtod: after oral administ ... | 2010 | 20184950 |
| discovery and comparative profiling of micrornas in a sweet orange red-flesh mutant and its wild type. | red-flesh fruit is absent from common sweet orange varieties, but is more preferred by consumers due to its visual attraction and nutritional properties. our previous researches on a spontaneous red-flesh mutant revealed that the trait is caused by lycopene accumulation and is regulated by both transcriptional and post-transcriptional mechanisms. however, the knowledge on post-transcriptional regulation of lycopene accumulation in fruits is rather limited so far. | 2010 | 20398412 |
| light controls phospholipase a2alpha and beta gene expression in citrus sinensis. | the low-molecular weight secretory phospholipase a2alpha (csspla2alpha) and beta (cspla2beta) cloned in this study exhibited diurnal rhythmicity in leaf tissue of citrus sinensis. only csspla2alpha displayed distinct diurnal patterns in fruit tissues. csspla2alpha and cspla2beta diurnal expression exhibited periods of approximately 24 h; csspla2alpha amplitude averaged 990-fold in the leaf blades from field-grown trees, whereas cspla2beta amplitude averaged 6.4-fold. diurnal oscillation of csspl ... | 2010 | 20388744 |
| influence of different organic fertilizers on quality parameters and the delta(15)n, delta(13)c, delta(2)h, delta(34)s, and delta(18)o values of orange fruit (citrus sinensis l. osbeck). | to investigate the influence of different types of fertilizers on quality parameters, n-containing compounds, and the delta(15)n, delta(13)c, delta(2)h, delta (34)s, and delta(18)o values of citrus fruit, a study was performed on the orange fruit cv. 'valencia late' (citrus sinensis l. osbeck), which was harvested in four plots (three organic and one conventional) located on the same farm. the results demonstrated that different types of organic fertilizers containing the same amount of nitrogen ... | 2010 | 20184327 |