Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| rhodococcus equi causing bacteremia in an adult with acute leukemia. | we have reported a case of isolated bacteremia due to rhodococcus equi in an immunocompromised, non-hiv-infected patient with acute leukemia. this patient's illness demonstrates a rare presentation of an emerging opportunistic pathogen that may be potentially acquired via exposure to domestic horses or their habitat during periods of aggressive chemotherapeutic administration. the infection was successfully eradicated by treatment with erythromycin and rifampin. counseling immunocompromised pati ... | 1993 | 8434305 |
| rhodococcus equi infection in foals: a report of an outbreak on a thoroughbred stud in zimbabwe. | twenty-four foals were confirmed to be infected with rhodococcus equi on a private stud in zimbabwe over a two-year period. six mares had foals which were affected in each of the two years. all the foals were febrile and early cases were detected by this pyrexia. bronchopneumonia was only clinically detectable in advanced cases. in spite of energetic hygiene measures relating to pasture and housing management, the incidence was higher in the second year (23 per cent of foals born) than in the fi ... | 1993 | 8438546 |
| characterization of the genome of the rhodococcus rhodochrous bacteriophage njl. | temperate bacteriophage njl of rhodococcus rhodochrous has a 49-kb linear double-stranded dna with cohesive ends (cos). njl dna has unique target sites for hindiii and sspi, two target sites each for nhei and scai, and no cleavage site for axyi, drai, ecori, saci, and sphi. the single-stranded regions of cos ends were ligated to each other with t4 dna ligase, removed with mung bean nuclease, or blunted with the klenow large fragment of dna polymerase i; then the sequences of the cos ends were de ... | 1993 | 8439171 |
| nocardioform arsenic resistance plasmid characterization and improved rhodococcus cloning vectors. | a representative of a group of related rhodococcus arsenic resistance plasmids was characterized, locating the resistance genes and regions influencing host range and controlling copy number. this information, together with identification of antibiotic resistance determinants to replace the arsenic marker was used to construct rhodococcus-escherichia coli positive selection shuttle plasmids which, compared with those previously constructed, had half the size, a much higher copy number, and chlor ... | 1993 | 8441772 |
| two independently regulated cytochromes p-450 in a rhodococcus rhodochrous strain that degrades 2-ethoxyphenol and 4-methoxybenzoate. | a red-pigmented coryneform bacterium, identified as rhodococcus rhodochrous strain 116, that grew on 2-ethoxyphenol and 4-methoxybenzoate as sole carbon and energy sources was isolated. phylogenetic analysis based on the 16s rdna sequences indicates that the strain clusters more closely to other rhodococci than to other gram-positive organisms with a high g + c content. each of the abovementioned growth substrates was shown to induce a distinct cytochrome p-450: cytochrome p-450rr1 was induced b ... | 1993 | 8444808 |
| rhodococcus equi infection in hiv-infected patients. | 1993 | 8455149 | |
| [a pulmonary abscess due to rhodococcus equi in an aids patient]. | 1993 | 8465058 | |
| novel mechanism for the camp reaction between listeria monocytogenes and corynebacterium equi. | the synergistic hemolytic reaction between listeria monocytogenes, staphylococcus aureus and corynebacterium equi on blood agar plates was explored using a modified test-tube assay. a synergistic lysis of sheep red blood cells was observed with l. monocytogenes and either s. aureus or c. equi, using crude extracts. synergism was also found with l. monocytogenes and purified cholesterol oxidase from pseudomonas fluorescens and brevibacterium brevi. crude extracts of s. aureus sensitized sheep red ... | 1993 | 8466816 |
| 1,3,5-trihydroxybenzene biodegradation by rhodococcus sp. bpg-8. | the metabolic pathway used by rhodococcus sp. bpg-8 isolated from oil-rich soil in newfoundland for the degradation of 1,3,5-trihydroxybenzene (phloroglucinol) as a sole source of carbon and energy was determined. culture filtrates of cells grown on phloroglucinol detected 1,2,3,5-tetrahydroxybenzene when extracted and analyzed using gas chromatography--mass spectrometry, thin-layer chromatography, and ultraviolet spectrophotometry. nonaromatics were either derivatized with 2,4-dinitrophenylhydr ... | 1993 | 8467418 |
| recognition of pneumocystis carinii in foals with respiratory distress. | five 3-month-old foals presenting with fever and respiratory disease were found to have pulmonary abscesses with patchy to diffuse alveolar and interstitial pneumonia on post-mortem examination. all affected foals had evidence of rhodococcus equi infection and had few to abundant pneumocystis carinii cysts in the sections of affected lung. of the 5 foals examined radiographically, 3 had a distinct reticulonodular (miliary) pattern which may aid in the ante-mortem diagnosis of p. carinii pneumoni ... | 1993 | 8467767 |
| immunodeficiency and serious pneumonia in foals: the plot thickens. | 1993 | 8467784 | |
| microbial desulfurization of dibenzothiophene: a sulfur-specific pathway. | rhodococcus rhodochrous strain igts8 metabolizes dibenzothiophene, a model compound for organic sulfur in fossil fuels, in a sulfur-specific manner. two routes of desulfurization have been identified. under growth conditions, the intermediates are dibenzothiophene sulfoxide, dibenzothiophene sulfone, 2'-hydroxybiphenyl-2-sulfonate, and 2,2'-dihydroxybiphenyl. stationary phase cells produce 2-hydroxybiphenyl as the desulfurized product and use the 2'-hydroxybiphenyl-2-sulfinate, rather than the s ... | 1993 | 8467997 |
| purification and characterization of cytochrome p450rr1 from rhodococcus rhodochrous. | a soluble cytochrome p450 whose synthesis is induced by and that binds 2-ethoxyphenol was purified to apparent homogeneity from rhodococcus rhodochrous strain 116. the enzyme had a subunit molecular mass of 44.5 kda as determined by sds/page and a pi of 5.2. the electronic absorption spectrum indicates that the native cytochrome in the absence of substrate is predominantly in the low-spin state (13% high-spin state in 50 mm mops, ph 7.0 25 degrees c). 2-methoxyphenol binds to the cytochrome with ... | 1993 | 8477696 |
| [rhodococcus equi pulmonitis in hiv+. a review of the literature and a case report]. | 1993 | 8480037 | |
| development of a serologic panel for the recognition of nocardial infections in a murine model. | a murine model was used to develop a sensitive and specific serologic test for clinical and subclinical infections caused by nocardia. the following tests were used: (a) enzyme-linked immunosorbent assays (elisas) with culture filtrate and cytoplasmic extract antigens from nocardia asteroides; (b) elisa with n. asteroides trehalose dimycolate (cord factor); (c) indirect immunofluorescent antibody assay with whole cells of n. asteroides; and (d) western-blot analysis for the 54 to 55-kd, 36-kd, a ... | 1993 | 8495584 |
| rhodococcus. | 1993 | 8496585 | |
| involvement of interferon-gamma and tumor necrosis factor-alpha in host defense against rhodococcus equi. | rhodococcus equi is a facultative, intracellular, gram-positive coccobacillus increasingly reported as an opportunistic pathogen in aids patients. in vitro, splenic cells of noninfected euthymic mice produced tumor necrosis factor-alpha (tnf alpha) in greater amounts when incubated with live r. equi rather than with killed bacteria. in vivo, interferon-gamma (ifn-gamma) and tnf alpha serum levels of infected euthymic mice remained below the level of detectability. treatment of infected nude mice ... | 1993 | 8501339 |
| endogenous gamma interferon is essential in granuloma formation induced by glycolipid-containing mycolic acid in mice. | the present study examined the role of endogenous gamma interferon (ifn-gamma) in the formation of granulomas in mice which had been given a single intravenous injection of glycolipid-containing mycolic acid (trehalose 2,3,6'-trimycolate) purified from cell walls of rhodococcus aurantiacus (gordona aurantiaca) (gagm) in the form of liposome. the histological status of granuloma formation in the livers, spleens, and lungs of gagm-injected mice was studied at weeks 1 through 5, and the titers of e ... | 1993 | 8514390 |
| the intracellular bacterium rhodococcus equi requires mac-1 to bind to mammalian cells. | rhodococcus equi is a facultative intracellular bacterium of macrophages that causes disease in immunocompromised individuals, particularly those with aids. in this report, we demonstrate that r. equi binding to mammalian cells requires complement and is mediated primarily by the leukocyte complement receptor, mac-1. bacteria bind to macrophages poorly unless exogenous complement is added to the incubation medium. the addition of fresh nonimmune serum, which contains no detectable antibodies to ... | 1993 | 8514396 |
| chemotaxonomy and molecular taxonomy of some coryneform clinical isolates. | six reference strains of the genus brevibacterium as well as fifteen clinical isolates tentatively assigned to the genus using conventional biochemical methods, were the subject of chemotaxonomic and dna similarity studies. five of these clinical isolates were assigned either to the genera aureobacterium, mycobacterium, gordona or to rhodococcus on the basis of their dna mol% g+c, mycolic acid, amino-acid, sugar and menaquinone contents. among the ten remaining strains, six were not brevibacteri ... | 1993 | 8518510 |
| rhodococcus equi pneumonia and occult hiv infection. | 1993 | 8102451 | |
| failure of pulmonary clearance of rhodococcus equi infection in cd4+ t-lymphocyte-deficient transgenic mice. | pulmonary clearance of rhodococcus equi requires functional t lymphocytes. in this study, cd8+ t-lymphocyte-deficient transgenic mice cleared virulent r. equi from the lungs while infection in cd4+ t-lymphocyte-deficient transgenic mice persisted. although both cd4+ and cd8+ t cells function early in pulmonary defense against r. equi, clearance is dependent on cd4+ t lymphocytes. | 1993 | 8104903 |
| respiratory infections in the immunocompromised patient. | damage to local and systemic defences of the lungs makes the immunocompromised host vulnerable to inhaled microorganisms. the type of underlying disease and its associated immunodeficiency allow a high degree of accurate pathogen prediction. neutropenia is associated with gram-negative bacilli pneumonia. prolonged neutropenia increases the risk of aspergillosis and mucormycosis. cellular immunodeficiency is associated with pneumocystis carinii, legionella spp. and intracellular parasites includi ... | 1993 | 18611585 |
| bioluminescent flow sensor for l-phenylalanine determination in serum. | a highly sensitive and rapid bioluminescent flow sensor was developed for the determination of the content of l-phenylalanine (phe) in serum by monitoring the reduced form of nicotinamide adenine dinucleotide (nadh), produced by immobilized phenylalanine dehydrogenase (phedh), with bacterial bioluminescent enzymes immobilized on a separate nylon coil. the l-phedhs extracted from bacillus badius, bacillus sphaericus and rhodococcus sp. m 4 were investigated and the performances of the three immob ... | 1993 | 18965647 |
| isolation of rhodococcus equi from the liver abscess of a goat in trinidad. | 1993 | 17424274 | |
| stereospecificity of microbial hydrations of oleic acid to 10-hydroxystearic acid. | we recently described a simple method for ascertaining the stereochemical purities of hydroxy fatty acids (s. h. el-sharkawy, w. yang, l. dostal, and j. p. n. rosazza, appl. environ. microbiol. 58:2116-2122, 1992) based on the h-nuclear magnetic resonance spectral analysis of diastereomeric s-(+)-o-acetylmandelate esters of hydroxystearates. this report describes the stereochemistries of microbial hydrations of oleic acid to 10-hydroxystearic acid by nocardia aurantia (also known as rhodococcus ... | 1993 | 16348853 |
| novel allylic oxidation of alpha-cedrene to sec-cedrenol by a rhodococcus strain. | a bacterial strain, designated ksm-7358, that can use alpha-cedrene for growth was isolated. the strain was identified as a member of the genus rhodococcus and catalyzed the novel allylic oxidation of alpha-cedrene regiospecifically to produce (r)-10-hydroxycedrene (sec-cedrenol) with a very high yield. alpha-curcumene was also produced as a possible metabolite of sec-cedrenol. a possible pathway for the microbial conversion of alpha-cedrene to sec-cedrenol and alpha-curcumene is proposed. | 1993 | 16348930 |
| identification and cloning of genes involved in specific desulfurization of dibenzothiophene by rhodococcus sp. strain igts8. | the gram-positive bacterium rhodococcus sp. strain igts8 is able to remove sulfur from certain aromatic compounds without breaking carbon-carbon bonds. in particular, sulfur is removed from dibenzothiophene (dbt) to give the final product, 2-hydroxybiphenyl. a genomic library of igts8 was constructed in the cosmid vector plafr5, but no desulfurization phenotype was imparted to escherichia coli. therefore, igts8 was mutagenized, and a new strain (uv1) was selected that had lost the ability to des ... | 1993 | 16349035 |
| physicochemical cell surface and adhesive properties of coryneform bacteria related to the presence and chain length of mycolic acids. | the presence and chain length of mycolic acids of bacteria of the genera corynebacterium, rhodococcus, gordona, mycobacterium, and arthrobacter and of coryneform bacteria containing a type b peptidoglycan were related to the cell surface hydrophobicity of the bacteria, which in turn was related to adhesion of the cells to defined surfaces such as teflon and glass. the origin of the overall negative charge of these bacteria is discussed. | 1993 | 16349100 |
| selective desulfurization of dibenzothiophene by rhodococcus erythropolis d-1. | a dibenzothiophene (dbt)-degrading bacterium, rhodococcus erythropolis d-1, which utilized dbt as a sole source of sulfur, was isolated from soil. dbt was metabolized to 2-hydroxybiphenyl (2-hbp) by the strain, and 2-hbp was almost stoichiometrically accumulated as the dead-end metabolite of dbt degradation. dbt degradation by this strain was shown to proceed as dbt --> dbt sulfone --> 2-hbp. dbt at an initial concentration of 0.125 mm was completely degraded within 2 days of cultivation. dbt at ... | 1994 | 16349153 |
| aerobic mineralization of trichloroethylene, vinyl chloride, and aromatic compounds by rhodococcus species. | two rhodococcus strains which were isolated from a trichloroethylene (tce)-degrading bacterial mixture and rhodococcus rhodochrous atcc 21197 mineralized vinyl chloride (vc) and tce. greater than 99.9% of a 1-mg/liter concentration of vc was degraded by cell suspensions. [1,2-c]vc was degraded by cell suspensions, with the production of greater than 66% co(2) and 20% c-aqueous phase products and incorporation of 10% of the c into the biomass. cultures that utilized propane as a substrate were ab ... | 1994 | 16349184 |
| purification and characterization of an inducible s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r. | the widespread use and relative persistence of s-triazine compounds such as atrazine and simazine have led to increasing concern about environmental contamination by these compounds. few microbial isolates capable of transforming substituted s-triazines have been identified. rhodococcus corallinus nrrl b-15444 has previously been shown to possess a hydrolase activity that is responsible for the dechlorination of the triazine compounds deethylsimazine (6-chloro-n-ethyl-1,3,5-triazine-2,4-diamine) ... | 1994 | 16349190 |
| cesium accumulation and growth characteristics of rhodococcus erythropolis cs98 and rhodococcus sp. strain cs402. | growth and cesium accumulation characteristics of two cesium-accumulating bacteria isolated from soils were investigated. rhodococcus erythropolis cs98 and rhodococcus sp. strain cs402 accumulated high levels of cesium (approximately 690 and 380 mumol/g [dry weight] of cells or 92 and 52 mg/g [dry weight] of cells, respectively) after 24 h of incubation in the presence of 0.5 mm cesium. the optimum ph for cesium uptake by both rhodococcus strains was 8.5. rubidium and cesium assumed part of the ... | 1994 | 16349312 |
| adsorption of rhodococcus strain gin-1 (ncimb 40340) on titanium dioxide and coal fly ash particles. | rhodococcus strain gin-1 (ncimb 40340) can be used to enrich and isolate a titanium-rich fraction from coal fly ash. the gram-positive bacterium was isolated by its ability to adhere strongly and rapidly to suspended particles of pure titanium dioxide or coal fly ash. adsorption depends on the salt concentration and occurs in seawater. lowering of the salt concentration or washing of particles with pure water did not, however, cause desorption of the bacteria from tio(2) particles; this was achi ... | 1994 | 16349369 |
| efficient transformation of the cephamycin c producer nocardia lactamdurans and development of shuttle and promoter-probe cloning vectors. | a high transformation efficiency (1 x 10 to 7 x 10 transformants per mug of dna) of nocardia lactamdurans lc411 was obtained by direct treatment of mycelium with polyethylene glycol 1000 and cesium chloride. a variety of vectors from streptomyces lividans, brevibacterium lactofermentum, rhodococcus fascians, and a nocardia (amycolatopsis) sp. were tested; transformants could be obtained only with vectors derived from an endogenous plasmid of the amycolatopsis sp. strain dsm 43387. vectors carryi ... | 1994 | 16349436 |
| pulmonary malakoplakia related to rhodococcus equi occurring in the acquired immunodeficiency syndrome. | 1994 | 8107640 | |
| development of reactive arthritis and resistance to erythromycin and rifampin in a foal during treatment for rhodococcus equi pneumonia. | 1994 | 8542848 | |
| correlation between antibiotic resistance, phage-like particle presence, and virulence in rhodococcus equi human isolates. | rhodococcus equi is a gram-positive coccobacillus that appears to be emerging as a pulmonary pathogen in aids patients. in four human clinical isolates, two antibiotic resistance phenotypes were found to coexist: one beta-lactam resistant and the other beta-lactam susceptible. in vitro, beta-lactam-resistant mutants were obtained at a frequency of 1 x 10(-5) to 5 x 10(-5) from beta-lactam-susceptible strains on cephalothin-containing plates. neither beta-lactamase nor plasmid dna was detected in ... | 1994 | 8150945 |
| virulence of rhodococcus equi isolates from patients with and without aids. | rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. thirty-nine isolates of r. equi from immunocompromised patients with and without aids were analyzed for the presence of virulence plasmid dna, expression of 15- to 17-kda antigens, and their pathogenicities in mice. of the human isolates, eight contained an 85-kb virulence plasmid, expressed 15- to 17-kda antigens, and were virulent in mice. nineteen isolates carried cryptic plasmids of vari ... | 1994 | 8150957 |
| [slowly developing cavitated pneumonia in a patient with hiv infection]. | 1994 | 8155754 | |
| identification and characterization of a transmissible linear plasmid from rhodococcus erythropolis bd2 that encodes isopropylbenzene and trichloroethene catabolism. | rhodococcus erythropolis bd2, which is able to utilize isopropylbenzene as a sole carbon and energy source, was shown to contain a conjugative linear plasmid, pbd2. the estimated size of pbd2 is 208 to 212 kb. linear plasmid-deficient strains had lost both the isopropylbenzene degradation and trichloroethene degradation characteristics, as well as the arsenite resistance and mercury resistance phenotypes. reintroduction of pbd2 restored all four characteristics. conjugational transfer of pbd2 to ... | 1994 | 8161179 |
| the fas operon of rhodococcus fascians encodes new genes required for efficient fasciation of host plants. | three virulence loci (fas, att, and hyp) of rhodococcus fascians d188 have been identified on a 200-kb conjugative linear plasmid (pfid188). the fas locus was delimited to a 6.5-kb dna fragment by insertion mutagenesis, single homologous disruptive recombination, and in trans complementation of different avirulent insertion mutants. the locus is arranged as a large operon containing six open reading frames whose expression is specifically induced during the interaction with host plants. one pred ... | 1994 | 8169198 |
| humoral immune response to rhodococcus equi in aids patients with r. equi pneumonia. | 1994 | 8169421 | |
| the catechol 2,3-dioxygenase gene of rhodococcus rhodochrous ctm: nucleotide sequence, comparison with isofunctional dioxygenases and evidence for an active-site histidine. | in cell-free extracts of escherichia coli clones harbouring the 3.5 kb bg/ii fragment of plasmid ptc1 from rhodococcus rhodochrous ctm a catechol 2,3-dioxygenase (c23o) accepting both 3-methylcatechol and 2,3-dihydroxybiphenyl as substrates could be detected. the plasmid-encoded gene for c23o of r. rhodochrous ctm and its flanking regions were sequenced. in front of the gene a sequence resembling an e. coli promoter was identified, which led to constitutive expression of the cloned gene in e. co ... | 1994 | 8180697 |
| biotransformations catalyzed by the genus rhodococcus. | rhodococci display a diverse range of metabolic capabilities and they are a ubiquitous feature of many environments. they are able to degrade short-chain, long-chain, and halogenated hydrocarbons, and numerous aromatic compounds, including halogenated and other substituted aromatics, heteroaromatics, hydroaromatics, and polycyclic aromatic hydrocarbons. they possess a wide variety of pathways for degrading and modifying aromatic compounds, including dioxygenase and monooxygenase ring attack, and ... | 1994 | 8187203 |
| rhodococcus equi infections of humans. 12 cases and a review of the literature. | increased recognition of rhodococcus equi as a human pathogen has occurred since 1983, when the first review article summarized the world's literature of 12 cases. in this article, we present 12 cases from the university of oklahoma health sciences center and review 60 from the literature. most cases occur in immunocompromised hosts and present as chronic cavitary pneumonias. associated extrapulmonary disease is seen at diagnosis in 7% of patients with pneumonia, and relapse occurs at extrapulmo ... | 1994 | 8190035 |
| rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a mycobacterium library. | current methods for identifying mycobacteria by high-performance liquid chromatography (hplc) require a visual assessment of the generated chromatographic data, which often involves time-consuming hand calculations and the use of flow charts. our laboratory has developed a personal computer-based file containing patterns of mycolic acids detected in 45 species of mycobacterium, including both slowly and rapidly growing species, as well as tsukamurella paurometabolum and members of the genera cor ... | 1994 | 8195387 |
| comparison of oral erythromycin formulations in the horse using pharmacokinetic profiles. | the pharmacokinetic properties of four erythromycin formulations were compared in six adult horses after administration of single and multiple oral doses. formulations of erythromycin administered were estolate and phosphate given 37.5 mg/kg every 12 h and 25 mg/kg every 8 h, and stearate and ethylsuccinate given 25 mg/kg every 8 h. areas under the curve (auc) and maximum plasma erythromycin concentrations (cmax) were equal or greater (p > or = 0.05) following administration of erythromycin phos ... | 1994 | 8196089 |
| sequences of the cobalamin biosynthetic genes cobk, cobl and cobm from rhodococcus sp. ni86/21. | sequence analysis of a 5753-bp genomic fragment of rhodococcus sp. ni86/21 revealed the presence of three homologues of known cobalamin biosynthetic genes. one of these genes encodes a protein showing strong homology with the precorrin-6x reductase (cobk) of pseudomonas denitrificans. in addition, the rhodococcal homologue of the corrin methyltransferases, cobm from p. denitrificans and cbif from salmonella typhimurium, was identified. the protein deduced from a third rhodococcus gene aligns wel ... | 1994 | 8200543 |
| effect of growth temperature on maintenance of virulent rhodococcus equi. | repeated passage of virulent rhodococcus equi atcc 33701 and l1 at 38 degrees c resulted in attenuation of the strains as a result of curing the virulence plasmid; at 30 degrees c, repeated passage had no such effect. at a temperature of 38 degrees c the plasmid-bearing cells replicated more slowly than their plasmid-cured derivatives and so were gradually replaced by cells lacking plasmids. in contrast, at a temperature of 30 degrees c the growth rate of either strain was not affected by the pr ... | 1994 | 8203124 |
| cloning, sequencing, and expression of rhodococcus l-phenylalanine dehydrogenase. sequence comparisons to amino-acid dehydrogenases. | l-phenylalanine dehydrogenase catalyzes the nad(+)-dependent, reversible, oxidative deamination of l-phenylalanine to form ammonia, phenyl pyruvate, and nadh. the enzyme has been purified to homogeneity from rhodococcus sp. m4, and a partial amino acid sequence was obtained. a cosmid library of rhodococcus sp. m4 genomic dna was prepared and used to isolate a 2.5-kilobase psti fragment that contained the pdh gene. the open reading frame of 1068 nucleotides encodes a polypeptide of 356 amino acid ... | 1994 | 8206922 |
| rhodococcus zopfii sp. nov., a toxicant-degrading bacterium. | a toluene-degrading bacterial strain isolated from bioreactors was identified as a member of the genus rhodococcus on the basis of the following characteristics: meso-diaminopimelic acid, arabinose, galactose, and glucose are the diagnostic cellular sugars; the mycolic acids contain 33 to 36 carbon atoms; and the formation of a branching mycelium is followed by marked fragmentation of the mycelium into irregular rod-shaped to coccoid units. dna-dna hybridization analyses performed with type stra ... | 1994 | 8123552 |
| identification of dna involved in rhodococcus chromosomal conjugation and self-incompatibility. | genes essential for rhodococcus chromosomal conjugation were found to be located on an unstable genetic element in one of the strains investigated. from this element two segments of dna could be identified which were involved in conjugation of rhodococcus strains. one region, spread over 8 kb, was involved in the property of self-incompatibility. a second region, of about 3 kb, was essential for conjugation between atcc 12674 and related nocardioforms. | 1994 | 8125246 |
| analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenases found in rhodococcus globerulus p6. identification of a new family of extradiol dioxygenases. | the polychlorobiphenyl-degrading bacterium rhodococcus globerulus p6 contains three bphc genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenases. one of them, bphc1, is clustered with the bphb gene which encodes 2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase and constitutes part of the bph operon specifying the degradation of biphenyl. the nucleotide sequence of bphb and the three bphc genes has been determined. the protein products of the bphbc1 gene cluster were found to exhibit significant ... | 1994 | 8126007 |
| structural elucidation of the specific capsular polysaccharide of rhodococcus equi serotype 7. | structural analysis of the specific capsular polysaccharide produced by rhodococcus equi serotype 7 indicated it to be a high-molecular-weight polymer consisting of equal molar amounts of d-galactose, d-mannose, l-rhamnose, and pyruvic acid. by employing a combination of chemical and nmr techniques, it was established that the polysaccharide is composed of the linear repeating trisaccharide units: [formula: see text] -->3)-alpha-d-galp-(1-->3)-alpha-d-manp-(1-->3)-alpha-l-rhap -(1-->, in which t ... | 1994 | 8137362 |
| spectroscopic observation of the intramolecular electron transfer in the photoactivation processes of nitrile hydratase. | the photoactivation phenomena of the photosensitive enzyme nitrile hydratase (nhase) was studied by various spectroscopic methods. we have already shown that the photoactivation of nhase accompanies oxidation of an iron atom in the nhase [honda et al. (1992) febs lett. 301, 177-180]. from the results obtained in the present study by absorption, action, and fluorescence spectra, we show that the chromophore responsible for the photoactivation process is the iron complex, and the tryptophan residu ... | 1994 | 8142355 |
| rhodococcus equi vertebral osteomyelitis in foals. | 1994 | 8143655 | |
| rhodococcus equi vertebral osteomyelitis in 3 quarter horse colts. | 1994 | 8143671 | |
| vertebral body osteomyelitis due to rhodococcus equi in two arabian foals. | 1994 | 8143672 | |
| [pneumonia caused by rhodococcus equi and salmonella spp. in a patient with human immunodeficiency virus infection]. | 1994 | 8059038 | |
| [rhodococcus equi infection in aids: a case with pulmonary abscess. review of the literature]. | rhodococcus equi is an easily missed opportunistic infection in patient infected by the human immunodeficiency virus. we report one case with lung abscess in a patient with aids with literature review. | 1994 | 8059147 |
| [pneumonia by rhodococcus equi in patients with hiv infection]. | we present the case of a patient with aids who developed abscessified pneumonia by rhodococcus equi. we publish this case given the rare frequency of presentation of this opportunistic infection in this type of patient and given its good clinical evolution, on the contrary of other cases described so far in the literature. the good evolution of the patient may be due to an early diagnosis, less than one month, and the combined medical therapy with rifampicine and tetracyclines. | 1994 | 8061140 |
| transfer of polychlorophenol-degrading rhodococcus chlorophenolicus (apajalahti et al. 1986) to the genus mycobacterium as mycobacterium chlorophenolicum comb. nov. | three independently isolated polychlorophenol-degrading strains of bacteria were characterized on the basis of chemotaxonomic and nutritional characteristics. previously, these strains were assigned to the species rhodococcus chlorophenolicus, which was described on the basis of the properties of one of the strains, strain pcp-i(t) (t = type strain) (j. h. a. apajalahti, p. kärpänoja, and m. s. salkinoja-salonen, int. j. syst. bacteriol 36:246-251, 1986). however, the results of analyses of myco ... | 1994 | 8068542 |
| sequence of rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an apc-like permease. | sequence analysis of a 5173-bp genomic fragment from the nocardioform actinomycete rhodococcus sp. strain ni86/21 revealed the presence of two genes, eutb and eutc, encoding the putative homologues of the large and small subunits of the ethanolamine ammonia-lyase, respectively, from salmonella typhimurium. this is the first report of the characterization of these genes in a gram-positive species. immediately upstream of eutb, a gene encoding a putative permease of the apc (amino acids, polyamine ... | 1994 | 8069783 |
| haloalkane degradation and assimilation by rhodococcus rhodochrous ncimb 13064. | the bacterium rhodococcus rhodochrous ncimb 13064, isolated from an industrial site, could use a wide range of 1-haloalkanes as sole carbon source but apparently utilized several different mechanisms simultaneously for assimilation of substrate. catabolism of 1-chlorobutane occurred mainly by attack at the c-1 atom by a hydrolytic dehalogenase with the formation of butanol which was metabolized via butyric acid. the detection of small amounts of gamma-butyrolactone in the medium suggested that s ... | 1994 | 8081504 |
| transthoracic needle aspiration in the study of pulmonary infections in patients with hiv. | to evaluate the safety and efficacy of transthoracic aspiration with an ultrathin needle in the microbiologic diagnosis of pulmonary infections in hiv-infected patients. | 1994 | 8082342 |
| enzyme-catalysed enantioselective hydrolysis of racemic naproxen nitrile. | the bacterial strain rhodococcus butanica (atcc 21197), which exhibits nitrilase and nitrile hydratase/amidase activities, catalyses the enantioselective hydrolysis of racemic naproxen nitrile (r/s)-1 to furnish a moderate enantiomeric excess of (s)-naproxen (s)-3. racemic naproxen amide (r/s)-2 is not a good substrate for this strain. resting cells of the newly selected bacterial strain rhodococcus sp. c3ii catalyse the enantioselective hydrolyses of racemic naproxen nitrile (r/s)-1 and naproxe ... | 1994 | 7858980 |
| rhodococcus equi infection in hiv-positive patients: report of 5 cases and literature overview. | the importance of rhodococcus equi infection, an uncommon human pathogen that almost exclusively affects immunocompromised hosts, has greatly increased following the advent of acquired immune deficiency syndrome (aids) epidemics. until the present time, 38 cases of r. equi infection have been described in human immunodeficiency virus (hiv)-infected patients; we now report a further five personal cases. r. equi was acquired via respiratory exposure to animals in less than half of the patients, an ... | 1994 | 7841972 |
| [distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms]. | the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ... | 1994 | 7898396 |
| bacteria and pesticides: a new aspect of interaction--involvement of a new biofactor. | positively charged hydrophobic pesticides of the dipyridyl family [diquat, paraquat, benzylviologen (bv++), etc.] were shown to provoke accumulation of 2-methylbutane-1,2,3,4-tetraol-2,4- cyclopyrophosphate in the cells corynebacterium (brevibacterium) ammoniagenes while neutral dipyridyls were not. hydrophobicity was also an important factor in this phenomenon. of the other pesticides tested, only linuron was effective. bv++ also induced biosynthesis of the compound in rhodococcus rhodochrous, ... | 1994 | 7916959 |
| detection of a genetic locus encoding resistance to rifampin in mycobacterial cultures and in clinical specimens. | the polymerase chain reaction (pcr) and automated dna sequencing were used to detect a genetic locus, rpob, associated with rifampin resistance in mycobacterium tuberculosis (tb) in clinical isolates and directly in clinical specimens. primers derived from the sequence of a tb rpob gene fragment were used to amplify dna from bacterial and mycobacterial isolates. an rpob-specific pcr product was obtained for five of five tb, seven of eight other mycobacterial species, nocardia sp., corynebacteriu ... | 1994 | 7924218 |
| tamponade due to rhodococcus equi in acquired immunodeficiency syndrome. | infection with rhodococcus equi has been described as a cause of cavitary pneumonia in aids patients. we report such a case, complicated by bacteremia, tamponade, and possible brain and liver abscesses. medical treatment was successful with pericardiocentesis and antibiotherapy with teicoplanin, gentamicin, clarithromycin, rifampicin, supplemented by empirical treatment of cerebral toxoplasmosis. antibiotherapy was terminated after 6 months, without relapse 2 months later. | 1994 | 7924515 |
| survival and replication of rhodococcus equi in macrophages. | rhodococcus equi is a facultative intracellular bacterium of macrophages that can cause serious pneumonia in both young horses and immunocompromised people. essential to understanding rhodococcus pathogenesis is a quantitative documentation of the intracellular events that follow macrophage phagocytosis of the organism. by using a bacterial immunofluorescence staining assay, we verified the intracellular survival and replicative potential of r. equi in both murine peritoneal macrophages and equi ... | 1994 | 7927672 |
| phosphorylative inactivation of rifampicin by nocardia otitidiscaviarum. | rifampicin was inactivated by nocardia otitidiscaviarum to yield two products designated rip-3 and rip-4. the full structures of rip-3 and rip-4 were determined to be 21-(o-phosphoryl) rifampicin and 3-formyl-21-(o-phosphoryl) rifamycin sv, respectively, but neither product had any antimicrobial activity. | 1994 | 7928806 |
| [new macrolides]. | the macrolides are still widely used in general practice and the new 14- or 15 membered-ring compounds-roxithromycin, clarithromycin, dirithromycin, azithromycin -may offer a new development. their antibacterial activity is similar to erythromycin but azithromycin is more active against gram negative strams. roxythromycin exhibits high serum concentrations; azithromycin and dirithromycin present low serum concentrations but prolonged half-lives allowing a reduction of the duration of treatment p ... | 1994 | 7939238 |
| pulmonary malacoplakia associated with rhodococcus equi infection in a patient with acquired immunodeficiency syndrome. | pulmonary malacoplakia associated with rhodococcus equi, an opportunistic gram-positive coccobacillus, is unusual. although this patient is the ninth reported to have pulmonary malacoplakia, he is only the third patient with the acquired immunodeficiency syndrome who has been reported to have pulmonary malacoplakia associated with r equi infection. the patient was a 49-year-old man infected with the human immunodeficiency virus who on initial examination was found to have cavitary pneumonia in t ... | 1994 | 7942603 |
| purification and characterization of an amidase from an acrylamide-degrading rhodococcus sp. | a constitutively expressed aliphatic amidase from a rhodococcus sp. catalyzing acrylamide deamination was purified to electrophoretic homogeneity. the molecular weight of the native enzyme was estimated to be 360,000. upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified preparation yielded a homogeneous protein band having an apparent molecular weight of about 44,500. the amidase had ph and temperature optima of 8.5 and 40 degrees c, respectively, and its isoelectric poin ... | 1994 | 7944367 |
| the enzymes of beta-oxidation in rhodococcus ruber. | 1994 | 7958285 | |
| bacterial oxidation of propane. | the bacterial metabolism of propane and the pathway(s) involved are poorly understood, as the relative importance of terminal versus subterminal oxidation of propane, via propan-1-ol and propan-2-ol, respectively, is still unclear. in the case of bacteria, the ability to oxidize propane appears to be confined mainly to the gram-positive corynebacterium - nocardia - mycobacterium - rhodococcus complex. studies on propane oxidation have been hampered by a lack of firm enzymological data; for examp ... | 1994 | 7958761 |
| characterization of the desulfurization genes from rhodococcus sp. strain igts8. | rhodococcus sp. strain igts8 possesses an enzymatic pathway that can remove covalently bound sulfur from dibenzothiophene (dbt) without breaking carbon-carbon bonds. the dna sequence of a 4.0-kb bstbi-bsiwi fragment that carries the genes for this pathway was determined. frameshift and deletion mutations established that three open reading frames were required for dbt desulfurization, and the genes were designated soxabc (for sulfur oxidation). each sox gene was subcloned independently and expre ... | 1994 | 7961424 |
| evaluation of api coryne system for identifying coryneform bacteria. | to identify rapidly and accurately coryneform bacteria, using a commercial strip system. | 1994 | 7962633 |
| [serodiagnosis of caseous lymphadenitis (pseudotuberculosis) in sheep]. | random postmortal isolation of corynebacterium pseudotuberculosis from the sheep used in a research project and the consequently determined serological positivity of these animals indicated the serological examination of the flock from which the sheep came. no symptoms of caseous lymphadenitis were ever observed in the flock in question and no data on the occurrence of this disease were available in the flock history. the sera of 228 head of sheep were used for examinations, while this number of ... | 1994 | 7975051 |
| transfer of rhodococcus aichiensis tsukamura 1982 and nocardia amarae lechevalier and lechevalier 1974 to the genus gordona as gordona aichiensis comb. nov. and gordona amarae comb. nov. | we investigated the taxonomic status of rhodococcus aichiensis dsm 43978t (t = type strain) and nocardia amarae dsm 43392t by using both chemotaxonomic and genetic methods. the occurrence of mk-9(h2) as the predominant menaquinone, the presence of relatively long-chain mycolic acids (48 to 62 carbon atoms), and the phylogenetic position as determined by a 16s ribosomal dna sequence analysis provide strong evidence that both r. aichiensis and n. amarae should be transferred to the genus gordona a ... | 1994 | 7981103 |
| identification of a heroin esterase in rhodococcus sp. strain h1. | a strain of a rhodococcus sp. (termed h1) capable of utilizing heroin as its sole carbon and energy source was isolated by selective enrichment. an inducible heroin esterase was partially purified and shown to catalyze the hydrolysis of both of the acetylester groups of heroin. the enzyme displays optimum activity at ph 8.5 and appears to be a trimer of identical subunits with an m(r) or 39,000 and a native m(r) of 120,000. | 1994 | 7986057 |
| brain abscess due to gordona terrae in an immunocompromised child: case report and review of infections caused by g. terrae. | a brain abscess complicated antineoplastic chemotherapy for a primary cerebral rhabdoid tumor in an immunocompromised boy. culture of purulent exudate obtained by surgical puncture of an intracranial hematoma yielded a gram-positive microorganism initially identified as a rhodococcus species by conventional biochemical analysis; however, the isolate was subsequently identified as gordona terrae by ribosomal dna analysis. to our knowledge, this is the third case of human infection caused by g. te ... | 1994 | 7986897 |
| rhodococcus equi pneumonia in an hiv-infected patient. | we report a case of a 46-year-old hiv-infected patient suffering rhodococcus equi pneumonia and septicaemia. after the failure of an initial antibiotic treatment, the upper lobe of the right lung was resected due to a cavitating pneumonia. after that the patient stabilized for a period of 7 months by administration of a resistogram-adapted combination of tetracycline, erythromycin and clindamycin. due to the patient's decision, antibiotic treatment was stopped when a cytomegalovirus retinitis wa ... | 1994 | 7987063 |
| review of 40 cases of lung abscesses in foals and adult horses. | a retrospective study of 40 horses with primary lung abscesses without pleuropneumonia showed that lung abscesses occurred predominantly in young horses, usually in foals aged < or = 6 months. hyperfibrinogenaemia was present in all but 2 horses, and other common clinical and haematological findings were hyperthermia, tachycardia, polypnoea, and neutrophilic leucocytosis. the most common bacterial species isolated from transtracheal aspirates were streptococcus zooepidemicus (20/34) and rhodococ ... | 1994 | 7988535 |
| haemolytic and phospholipase c (plc) activities of rhodococcus equi. | rhodococcus equi, an intracellular organism causing pneumonia and lung abscesses in foals, is generally thought to be non-haemolytic. in the present study, however, 13 of 14 representative isolates were found to be haemolytic when tested on agar media containing washed red blood cells rather than whole blood. red cells of rabbits, dogs, horses and man were more sensitive to lysis than were those of ruminants. two new enzymatic activities of the species were defined: a lecithinase and a phosphati ... | 1994 | 7989259 |
| evaluation of a monoclonal antibody-based colony blot test for rapid identification of virulent rhodococcus equi. | we recently generated a monoclonal antibody immunoglobulin g1 (mab 10g5), which can recognize 15- to 17-kda antigens, virulence-associated antigens of rhodococcus equi, and developed a colony blot enzyme-linked immunosorbent assay with mab 10g5 for the rapid identification of virulent r. equi. in this epidemiologic study, we evaluated the results of the colony blot test in the identification of virulent isolates of r. equi from feces of horses and soil and compared them with those from a convent ... | 1994 | 7999891 |
| permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes. | the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ... | 1994 | 8000549 |
| a kinetic study and application of a novel carbonyl reductase isolated from rhodococcus erythropolis. | the newly described carbonyl reductase from rhodococcus erythropolis (recr) accepts a broad range of substrates. based on the kinetic constants of a variety of methyl and ethyl ketones a hypothetical model of the substrate-binding site is proposed. whether a substrate of interest may be reduced by the recr can be predicted from this model together with the kinetic data. a study of initial velocities and product inhibition is presented, which shows that the kinetics of the recr follow a theorell- ... | 1994 | 8000863 |
| enzymatic asymmetric synthesis of alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols by arylalkyl acylamidases. | with the novel microbial enzyme, 'arylalkyl acylamidase', optically active alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols have been obtained through enantioselective hydrolysis of their racemic amides and esters. (s)-enantiomers of 1-methylbenzylamine, 1-methyl-3-phenylpropylamine and 1-methyl-3-phenylpropanol of high optical purity (> 94% e.e.) were synthesized with the cells of nocardia erythropolis iam 1440 or cellulomonas fimi aku 671. (r)-enantiomer of 1-methyl-3-phenylprop ... | 1994 | 8000864 |
| stereoselective epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile. | the chiral intermediate (3s,4r)-trans-3,4-dihydro-3,4-dihydroxy-2,2-dimethyl- 2h-1-benzopyran-6-carbonitrile [(+)-trans diol 3] was made by the stereoselective microbial epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile 1. this compound is a potential intermediate for the total synthesis of potassium-channel openers. several microbial cultures were found which catalyzed the transformation of 1 to the corresponding (3s,4s)-epoxide 2 and (+)-trans diol 3. the two best cultures, corynebact ... | 1994 | 8000876 |
| rapid degradation of the triazinone herbicide metamitron by a rhodococcus sp. isolated from treated soil. | seven bacterial isolates which degraded the herbicide metamitron (3-methyl-4-amino-6-phenyl-1,2,4-triazin-5-one) were obtained from field-enhanced soil by liquid enrichment culture. all isolates appeared to be identical and a representative, 0246b, was identified as a rhodococcus sp. by cell wall and fatty acid analyses. this isolate degraded metamitron as the sole source of carbon within 24 h at 25 degrees c and this is the first report of a bacterium capable of growing with metamitron as the s ... | 1994 | 8002472 |
| [emergent pathologies: rhodococcus equi infection in the acquired immunodeficiency syndrome]. | rhodococcus equi is an intracellular facultative, gram-positive, partially acid-fast, cocco-bacillary microorganism of increasing importance as a pathogen in severely immunocompromised hiv-positive patients. rhodococcus equi may cause invasive pulmonary infection, bacteremia and disseminated infection. rhodococcus equi is easily cultured from blood or sputum on standard media, but is frequently regarded as a contaminant. delay in establishing a definite diagnosis may result in a poor outcome in ... | 1994 | 8003385 |
| molecular cloning and nucleotide sequence of the gene coding photosensitive nitrile hydratase. | 1994 | 8010666 | |
| fatal rhodococcus equi infection in an angora goat. | 1994 | 8011764 | |
| metabolism of styrene by rhodococcus rhodochrous ncimb 13259. | rhodococcus rhodochrous ncimb 13259 grows on styrene, toluene, ethylbenzene, and benzene as sole carbon sources. simultaneous induction tests with cells grown on styrene or toluene showed high rates of oxygen consumption with toluene cis-glycol and 3-methylcatechol, suggesting the involvement of a cis-glycol pathway. 3-vinylcatechol accumulated when intact cells were incubated with styrene in the presence of 3-fluorocatechol to inhibit catechol dioxygenase activity. experiments with 18o2 showed ... | 1994 | 8017910 |
| purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in rhodococcus ruber. | the n-terminal amino acid sequence of the polyhydroxyalkanoic acid (pha) granule-associated m(r)-15,500 protein of rhodococcus ruber (the ga14 protein) was analyzed. the sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated m(r) of 14,175 which was recently localized downstream of the pha synthase gene (u. pieper and a. steinbüchel, fems microbiol. lett. 96:73-80, 1992). a recombinant strain of escherichia coli xl1-b ... | 1994 | 8021220 |
| rhodococcus equi: first case in a heart transplant recipient. | rhodococcus equi is a gram-positive diphtheroid that occasionally affects immunocompromised patients, usually causing a chronic respiratory infection with cavitating pulmonary opacities on chest radiograph that resemble mycobacterial or fungal disease. etiologic diagnosis presents a number of pitfalls, because rhodococcus equi isolates mimic many of the characteristics of other microorganisms more familiar to the laboratory staff. the treatment of choice for this disease has not yet been establi ... | 1994 | 8031817 |