Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
an investigation of bacterial causes of arthritis in slaughter hogs.joints from 153 arthritic and 80 normal slaughter hogs were examined by culture for presence of bacteria. although none of the normal joints yielded bacteria, 37% of the disease joints were positive for bacterial growth. of 67 bacterial isolates obtained, 45% were erysipelothrix rhusiopathiae. occurrence of other bacteria in order of their frequency was streptococcus suis (16%), actinomyces pyogenes (10%), mycoplasma spp. including 3 m. hyorhinis isolates (7%), staphylococci (7%), streptococcus ...19921554766
monoclonal antibodies preventing the development of polyarthritis in rats induced by experimental infection with erysipelas bacteria.monoclonal igm-antibodies specific for arthritogenic erysipelas bacteria (erysipelothrix rhusiopathiae, serovar 2, strain t28) were isolated from rats suffering from erysipelas polyarthritis. four of them (c52, d9, e51, r117) were administered to syngeneic lewis rats. while d9 and an unrelated rat-igm-antibody showed no effect, c52 protected partially and r117 as well as e51 fully from all symptoms of erysipelas polyarthritis. prevention of the disease was associated with a lack of antibody-form ...19921570491
changes in rat leukocyte populations in peripheral blood, spleen, lymph nodes, and synovia during erysipelas bacteria-induced polyarthritis.kinetics of leukocyte subsets were followed for several weeks in rats suffering from polyarthritis induced by experimental infection with erysipelas bacteria (erysipelothrix rhusiopathiae, serovar 2, strain t28). a marked leukocytosis was found in peripheral blood, and, with some delay, in the synovia and draining lymph nodes of affected joints. in the lymphoid organs tested considerable blast formation of lymphoid cells with a paucity of polymorphonuclear granulocytes was found, while the latte ...19921606200
erysipelothrix rhusiopathiae septicemia-polyserositis and streptococcal encephalitis in a calf. 19921616989
serological assay for swine erysipelas using nitrocellulose particles impregnated with an immunodominant 65 kda antigen from erysipelothrix rhusiopathiae.pigs (n = 10) that were experimentally challenged with an arthritogenic isolate of erysipelothrix rhusiopathiae (strain vrs 229; serotype 1a) developed arthritis in at least one of twelve major limb joints. immunoblots using sera obtained from these pigs at necropsy revealed a major band of immunoreactivity against a subunit polypeptide of apparent molecular mass 65 kda. the usefulness of the 65 kda immunodominant subunit as an assay reagent in an elisa test was examined by presentation of antig ...19921626367
[the development of a technology for the production of live dried vaccines against avian pasteurellosis and swine erysipelas].the technology of the production of dried live vaccine against pasteurella infection of fowl from pasteur's 2nd avirulent strain, strains ab and k, has been developed. this technology includes the process of batch cultivation of pasteurella cells, controlled in such parameters as eh, po2 and glucose concentration, in fermenters in optimized culture medium, based on hottinger hydrolysate and fermentative casein-yeast hydrolysate, and preservation in improved saccharose-gelatin medium prepared in ...19938067095
persistent bacteremia with erysipelothrix rhusiopathiae in a hospitalized patient.a patient with a history of alcohol abuse and pancreatitis presented with a pleural effusion resulting from a fistula between the pancreatic duct and left pleural space. two weeks into her hospitalization, fever and persistent bloodstream infection with erysipelothrix rhusiopathiae and candida albicans developed. the patient had no history of exposure to animals. to our knowledge this is the first report of an e. rhusiopathiae infection presenting during hospitalization. this case suggests the p ...19938268364
prophylactic application of propionibacterium avidum kp-40 in swine with acute experimental infections. ii. bacterial infections: pleuropneumonia and swine erysipelas.the potent immunomodifier propionibacterium avidum kp-40 (pa) demonstrated prophylactic potency in swine infected experimentally with haemophilus (actinobacillus) pleuropneumoniae or erysipelothrix rhusiopathiae. animals received pa either together with the respective vaccine or pa only; 3 resp. 4 weeks later all animals were inoculated with virulent pathogens. eight of 10 swine immunized with inactivated pleuropneumonia vaccine developed mild-moderate forms of infection with temporary stagnatio ...19938319545
nucleotide sequence analysis and heterologous expression of the erysipelothrix rhusiopathiae dnaj gene.dna sequence analysis of chromosomal dna from the gram-positive facultative intracellular pathogen, erysipelothrix rhusiopathiae has identified a dnaj heat shock gene homolog. a 1109-bp open reading frame encoding dnaj is located immediately 3' to the e. rhusiopathiae dnak gene. the deduced dnaj amino acid sequence exhibits the modular structure of other members of the dnaj protein class including a glycine-rich region and the repeating consensus sequence cxxcxgxgx. heterologous expression of th ...19938359682
comparison of etiological and immunological characteristics of two attenuated erysipelothrix rhusiopathiae strains of serotypes 1a and 2.two acriflavine-fast attenuated erysipelothrix rhusiopathiae strains koganei 65-0.15 of serotype 1a (strain kg-1a) and 2 (strain kg-2) were comparatively characterized. biochemical characterization showed the similar reactions with slight variation between the strains. strain kg-2 was more resistant to acriflavine dye than strain kg-1a. pathogenicity of strain kg-2 was higher than strain kg-1a in mice of strains ddy. c3h/he and a/j. significant differences of clinical signs between strains kg-1a ...19938399739
cloning, heterologous expression, and characterization of the erysipelothrix rhusiopathiae dnak protein.the dnak (hsp70) gene from the facultative intracellular pathogen erysipelothrix rhusiopathiae was cloned by heterologous dna hybridization of a genomic library using the escherichia coli dnak gene as a probe. a 3.2-kb fragment which encoded an 1,800-bp open reading frame was recovered. the deduced amino acid sequence of this open reading frame shares 56% identity with the e. coli dnak protein. expression of the encoded protein in e. coli by using the phage t7 promoter/polymerase system resulted ...19938423071
differentiation of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cell proteins.the protein patterns of whole cells of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum were studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the protein patterns of the 16 strains of e. rhusiopathiae and e. tonsillarum studied, including the type strains of these two species, resembled each other, except that there were 71-, 41-, 34-, and 26-kda proteins in the e. rhusiopathiae pattern and 74-, 44-, 36-, and 25-kda proteins in the e. tonsillarum pattern. th ...19938427802
erysipelas in caged laying chickens and suspected erysipeloid in animal caretakers.erysipelas was diagnosed in 2 succeeding caged layer flocks housed in the same building and was characterized clinically by sudden death. at necropsy, lesions comprised generalized congestion, hemorrhages in the skeletal muscles and visceral organs, and swelling of liver, spleen, and kidney with or without large irregular zones of necrosis. focal to massive hepatic necrosis was seen histologically with minimal or no inflammatory reaction. erysipelothrix rhusiopathiae was cultured from internal o ...19938507697
detection of plasmid dna in erysipelothrix rhusiopathiae isolated from pigs with chronic swine erysipelas.forty-three strains of erysipelothrix rhusiopathiae, isolated from pigs with chronic swine erysipelas, were examined for the presence of plasmid dna by agarose gel electrophoresis and electron microscopy. seven of these strains were found to contain plasmids of which number were varied from 1 to 6. the plasmids ranged from 1.4 to 86 kb in size. this is the first reported evidence for plasmid dna in e. rhusiopathiae. the functions of the plasmids are unknown at present.19938513023
properties of a protective protein antigen of erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae is a widely distributed mucosal commensal of the alimentary tracts of vertebrates. antibodies to a 66-64 kda protein released from the cell surface have been shown to be involved in protective immunity. mice immunized with the purified 66-64 kda protein from strain t28, serotype 2b were protected against challenge by the united states challenge strain e1-6p (serotype 1a) and by the official german challenge strain frankfurt 1 (serotype n). thus, protection is not ser ...19938116193
analysis of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum by multilocus enzyme electrophoresis.the genetic diversity of 74 australian field isolates of erysipelothrix rhusiopathiae and 22 reference strains for serovars of e. rhusiopathiae or erysipelothrix tonsillarum was examined by multilocus enzyme electrophoresis. four serovar reference strains of e. tonsillarum (strains ks 20 a, wittling, lengyel-p, and bano 107 for serovars 25, 3, 10, and 22, respectively) were genetically distinct from e. rhusiopathiae. however, the e. tonsillarum reference strain for serovar 14 (iszap-4) and the r ...19948150944
[bacterial endocarditis due to erysipelothrix rhusiopathiae. a clinical case report].the authors describe an unusual case of bacterial endocarditis. a very rare etiologic agent, erysipelothrix rhusiopathiae, was isolated. the patient was not fisherman or butcher and he did ot other risky job. moreover the disease had an atypical course.19948028747
direct and rapid detection of erysipelothrix rhusiopathiae dna in animals by pcr.erysipelothrix rhusiopathiae is a gram-positive rod capable of causing erysipelas in swine. to establish a method for specifically detecting e. rhusiopathiae for practical applications, such as for the inspection of slaughterhouses, the feasibility of using primers derived from the dna sequence coding for 16s rrna in a pcr-specific detection system was investigated. oligonucleotide primers were designed to amplify a 407-bp dna fragment by pcr. the amplification was specific to the erysipelothrix ...19947521358
alpha-mannosidase: a rapid test for identification of arcanobacterium haemolyticum.a 4-h alpha-mannosidase test for identification of arcanobacterium haemolyticum strains (n = 139) and differentiation of a. haemolyticum from actinomyces pyogenes strains (n = 30) and other gram-positive rods was evaluated. practically all a. haemolyticum strains (138 of 139) and the listeria monocytogenes type strain were alpha-mannosidase positive, while all a. pyogenes strains and corynebacterium (n = .25) strains as well as the rhodococcus equi and erysipelothrix rhusiopathiae type strains w ...19947710479
intercellular adhesion molecule-1 (icam-1) and mhc class ii on chondrocytes in arthritic joints from pigs experimentally infected with erysipelothrix rhusiopathiae.this study set out to investigate the in vivo expression and distribution of the porcine homologues of the intercellular adhesion molecule-1 (icam-1) and mhc class ii as markers of chondrocyte activation during the development of chronic polyarthritis, which was experimentally induced in landrace pigs by intra-articular injection of erysipelothrix rhusiopathiae. icam-1 was found to be strongly expressed in vivo on chondrocytes and synovial cells in arthritic joints but was not detected in cartil ...19947866347
erysipelothrix rhusiopathiae, serotype 17, septicemia in moose (alces alces) from algonquin park, ontario.erysipelothrix rhusiopathiae septicemia was diagnosed in three of four moose found dead in algonquin provincial park, ontario, canada, in the spring of 1989. type 17 e. rhusiopathiae was isolated from liver, lung, kidney, and lymph nodes of affected animals, which were in poor body condition, and suffering hair loss associated with tick (dermacentor albipictus) infestations. microscopic lesions consisted of mild, multifocal, necrotizing myocarditis, sarcocystosis, and lymph node atrophy. the bac ...19947933291
erysipelothrix rhusiopathiae infection in an emu (dromaius novaehollandiae). 19947948213
[endocarditis from erysipelothrix rhusiopathiae].erysipelothrix rhusiopathiae only seldom causes cases of endocarditis. contact with infectious animals leads to endocarditis of the left heart with high lethality. vancomycin and aminoglycosides, which are often used in gram-positive endocarditis, show no effect.19947982715
presence of a capsule in erysipelothrix rhusiopathiae and its relationship to virulence for mice.three avirulent insertional mutants of erysipelothrix rhusiopathiae were obtained by the technique of transposon mutagenesis with the self-conjugative transposon tn916. the interactions between murine polymorphonuclear leukocytes and parent and mutant strains were studied in vitro. in the presence of normal serum, the virulent parent strain was resistant to phagocytosis, whereas the avirulent mutant strains were efficiently phagocytosed. in the presence of immune serum, the parent and the mutant ...19948005671
[erysipelothrix rhusiopathiae septicemia]. 19948059140
cellular fatty acid composition of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum.the cellular fatty acid compositions in 6 strains of erysipelothrix rhusiopathiae and 7 strains of erysipelothrix tonsillarum were determined by gas chromatography. fatty acids, ranging from c10 to c18, were detected in the test strains. the fatty acid profile was characterized by very high percentages of 18:1 (cis-9) (cis-9-octadecenoic acid; 72.4 to 82.1%) and 16:1 (hexadecenoic acid; 8.7 to 13.7%). the profiles of the e. rhusiopathiae and e. tonsillarum strains resembled each other, indicatin ...19948075232
[erysipelothrix rhusiopathiae endocarditis with atrioventricular fistula]. 19948092652
[newer knowledge concerning a protective antigen of erysipelothrix rhusiopathiae]investigations on extracts from erysipelothrix (e.) rhusiopathiae carried out within the last few years yielded the identification of a species-specific proteinaceous antigen of 66-64 kda. the protective properties of this protein presented in particular in crude naoh and naoh-edta but not in acid and heat extracts could be demonstrated in mice and pigs vaccinated with the electroeluted 66-64 kda antigen or treated with polyclonal and monoclonal antibodies, respectively. the identification and c ...199411178405
[potency testing of erysipelas vaccines in mice. elisa vs. challenge]vaccines are widely used for the prophylaxis of swine erysipelas. according to national pharmacopoiea requirements, potency of these biologicals has to be tested in a mouse challenge model. many animals are needed and the test causes much suffering. the development of an enzyme linked immunosorbet assay (elisa) using an alkaline extract of erysipelothrix rhusiopathiae as antigen for coating the microtitre plate allows us to measure antibody titres in the serum of vaccinated mice. by comparing th ...199411178406
comparison of the pathogenicity for chickens of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum.twenty-two strains of erysipelothrix rhusiopathiae, 14 strains of erysipelothrix tonsillarum, and four other strains representing two serovars were examined for virulence in 30- to 40-day-old specific pathogen-free chickens. some of the chickens that were given strains of serovars la, 2, 5, 6, 8, 9, 15 or 21 of e. rhusiopathiae showed signs of disease, lesions, bacteraemia or recovery of the bacterium. in contrast, there were no clinical signs, pathological lesions or bacterial isolations in chi ...199418671089
non-purulent arthritis in danish slaughter pigs. i. a study of field cases.the pathology and microbiology of non-purulent arthritis in danish slaughter pigs were studied in order to draw up an updated picture of the disease. samples of one or more joints, their regional lymph nodes and the heart were collected from 101 arthritic pigs, totalling 137 joints, 86 lymph nodes and 96 hearts (group i). for another selected group including 25 pigs (group ii), the preliminary diagnosis of mycoplasmic arthritis, based on the appearance of the corresponding regional lymph node, w ...19958822187
protective activity and antigenic analysis of fractions of culture filtrates of erysipelothrix rhusiopathiae.the protective activity in mice and antigenic composition of the culture filtrate of 11 strains of erysipelothrix rhusiopathiae were compared. protective activity was found in the first (p-1) fraction obtained by sephadex g-200 gel filtration of the culture filtrate of each strain. comparing the 50% protective dose (pd50) of the p-1 fraction of the 11 strains by active immunization, highly protective activity was shown by 5 strains, such as agata, fujisawa, shizuoka-63, koganei 65-0.15 and se-9. ...19957740756
difficulties encountered in identification of a nutritionally deficient streptococcus on the basis of its failure to revert to streptococcal morphology.difficulties were encountered in the recognition of a nutritionally deficient streptococcus which continued to display aberrant morphologic forms (especially bulbous swellings and filament formation) despite provision of growth factors. with isolates displaying diverse morphologic entities not characteristic of a given species, e.g., streptobacillus moniliformis or erysipelothrix rhusiopathiae, nutritionally deficient streptococci should be considered.19957790428
erysipelas in quail and suspected erysipeloid in processing plant employees.erysipelas was diagnosed in two commercial breeder flocks of coturnix quail and was characterized clinically by sudden mortality. at necropsy, lesions comprised generalized congestion, hemorrhages in the thigh and breast muscles, swelling of liver, spleen, and kidney, and dark red edematous lungs. erysipelothrix rhusiopathiae was cultured from visceral organs of affected birds. medication with penicillin in the drinking water controlled the mortality. a possible source of infection was fish rais ...19957794182
localization of viable bacteria and bacterial antigens in arthritic joints of erysipelothrix rhusiopathiae-infected pigs.chronic polyarthritis was induced in pigs by infection with erysipelothrix rhusiopathiae (serovar 2, strain t28). viable bacteria could be reisolated as long as 5 months post-infection from synovial fluid, synovial tissue and from isolated chondrocytes. the number of viable bacteria could be increased by hypotonic shock of the chondrocytes indicating a substantial intracellular amount of bacteria. bacterial antigens were shown by immunohistochemistry to be present on the surface of both chondroc ...19958589663
classification of erysipelothrix strains on the basis of restriction fragment length polymorphisms.restriction fragment length polymorphisms of the 16s rrna genes of erysipelothrix strains were studied by cleavage of the chromosomal dna with restriction endonuclease ecori, followed by hybridization to a 420-bp internal fragment of the 16s rrna gene. thirty-two erysipelothrix type and reference strains were classified, together with seven field strains. reference strains of all serotypes and the type strains of erysipelothrix rhusiopathiae and erysipelothrix tonsillarum were included. nine rib ...19957537073
[erysipelothrix rhusiopathiae--a cause of erysipeloid and endocarditis].erysipelothrix rhusiopathiae is widespread among many species of animals including fish. erysipeloid is the most common infection caused by this bacterium in man; systemic infection, with endocarditis, is rare. most of the affected patients risk exposure to the organism at work. two patients with e. rhusiopathiae infections are described; one with erysipeloid after slaughtering a deer, the other with fatal endocarditis after gutting an eel. erysipeloid may be confused with "seal finger", but thi ...19957570494
antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens).the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ...19957587049
erysipeloid infection in a sheep farmer with coexisting orf.erysipeloid is a rarely reported zoonotic infection caused by erysipelothrix rhusiopathiae. we report a case of coexisting erysipeloid and orf infections in a sheep farmer, an association not previously recorded to our knowledge.19957636284
erysipelothrix rhusiopathiae bacteraemia in a patient without endocarditis. 19957636289
[infectious endocarditis caused by erysipelothrix rhusiopathiae]. 19957723720
[erysipelothrix rhusiopathiae]. 199610605243
intracellular survival and replication of erysipelothrix rhusiopathiae within murine macrophages: failure of induction of the oxidative burst of macrophages.we investigated the ability of a virulent wild-type parent strain and acapsular avirulent transposon mutants to enter and survive intracellularly within murine peritoneal macrophages. in the presence of normal or immune serum, the parent and mutant strains were both ingested; however, the number of ingested bacteria was three- to fourfold greater in the case of mutant strains than in the case of the parent strain. the parent strain, but not the mutant strains, survived and replicated intracellul ...19968613392
growth ability and immunological properties of erysipelothrix rhusiopathiae serotype 2.five field strains of erysipelothrix rhusiopathiae belonging to serotype 2 were compared for their growth ability, immunogenicity in mice, sds-page profile of cell surface proteins and their immunoblotting patterns. strain tama-96 showed the most stable growth in feist medium and tryptose phosphate broth with tween 80 (tpb), and its immunogenicity was highest in a mouse protection test using the inactivated vaccines prepared from 20-h tpb culture. the 50% mouse protective dose of the vaccine was ...19968645767
[bacteremia caused by erysipelothrix rhusiopathiae in an hiv-positive patient]. 19968756229
endocarditis and acute renal failure due to erysipelothrix rhusiopathiae. 19968781890
serovars of erysipelothrix strains isolated from pigs affected with erysipelas in japan.serovars of 1,046 erysipelothrix strains isolated from diseased pigs during a period from 1983 to 1993 in japan were determined by an agar gel double-diffusion precipitation system using typing sera representing all the known serovars, 1 through 23 and type n, of 2 species of erysipelothrix. a total of 943 strains could be serotyped within the serovars. the remaining 103 strains could not be determined for their serovars and were classified as untypable. of the 938 (99.5%) strains serologically ...19968811634
acute oliguric renal failure in a patient with an erysipelothrix rhusiopathiae bacteremia and endocarditis. 19968883054
erysipelothrix rhusiopathiae septicemia in systemic lupus erythematosus. 19968915741
characterization of spaa, a streptomyces coelicolor gene homologous to a gene involved in sensing starvation in escherichia coli.a streptomyces coelicolor gene, called spaa, homologous to the stationary phase regulatory gene rspa of escherichia coli [huisman and kolter (1994) science 265, 537-539], was cloned using the streptomyces ambofaciens rspa homologue spa2 [schneider et al. (1993) j. gen. microbiol. 139, 2559-2567] as a probe. considerable differences in sequence and in genetic context were detected between spa2 of s. ambofaciens and spaa of s. coelicolor. a cloned internal fragment of spaa was used to direct integ ...19968921874
[aortic endocarditis following erysipelothrix rhusiopathiae septicemia]. 19968949797
[erysipelothrix rhusiopathiae: plasmids, resistance to antibacterial drugs].the plasmid profile, virulence and antibacterial drug susceptibility of various strains of e. rhusiopathiae were determined. no correlations between the virulence of the strains, their antibiotic resistance and the plasmid content were detected. structural and functional analysis of one of the isolated plasmids was carried out to use the plasmid as a vector in the genetic study of e. rhusiopathiae.19968967808
[septic arthritis caused by erysipelothrix rhusiopathiae]. 19968991446
infective endocarditis caused by erysipelothrix rhusiopathiae in a patient with systemic lupus erythematosus. 19969251330
phenotypic and phylogenetic characterization of some eubacterium-like isolates containing a novel type b wall murein from human feces: description of holdemania filiformis gen. nov., sp. nov.a group of eubacterium-like strains (designated group s14), isolated from the feces of healthy people, was characterized by biochemical tests, fatty acid analysis, cell wall murein analysis, and 16s rdna analysis. our results indicate that group s14 is phylogenetically a member of the clostridium subphylum of the gram-positive bacteria. despite a phenotypic resemblance to the genus eubacterium, group s14 was shown to be phylogenetically distantly related to the type species of the genus, eubacte ...19979336928
concurrent infection with enteric protozoa and erysipelothrix rhusiopathiae in chicken and pheasant flocks. 19979347625
disseminated intravascular coagulation in chickens inoculated with erysipelothrix rhusiopathiae.in a first experiment, 28 specific pathogen-free chickens aged 3 weeks showed clinical signs 1 to 5 days after intramuscular inoculation with erysipelothrix rhusiopathiae. twelve of 28 birds died 2 to 4 days after inoculation. macroscopically, the liver, spleen and kidneys were seen to be enlarged and congested. histologically, fibrinous thrombus formation, seen in the hepatic sinusoids, renal glomerular capillaries and small pulmonary blood vessels, was a characteristic feature. in addition, th ...19979352439
erysipelothrix rhusiopathiae endocarditis: clinical features of an occupational disease.erysipelothrix rhusiopathiae is becoming more commonly recognized in humans and has the potential for significant morbidity and mortality. in this article, we describe one patient's clinical symptoms after occupational exposure to e rhusiopathiae and its sequela. we discuss the natural history of the organism, three major categories of human disease, and treatment options.19979386061
effect of attenuated erysipelothrix rhusiopathiae vaccine in pigs infected with porcine reproductive respiratory syndrome virus.twenty 2nd specific pathogen-free pigs were divided into 4 groups: group a were infected with porcine reproductive and respiratory syndrome (prrs) virus at 6 weeks of age and treated with available swine erysipelas and swine fever combined vaccine (vaccinated) at 7 weeks of age; group b were vaccinated at 7 weeks of age and infected with prrs virus at 8 weeks of age; group c were vaccinated at 7 weeks of age: group d were neither vaccinated nor infected with prrs virus. all pigs were challenged ...19979409511
bacterial isolation from slaughtered pigs associated with endocarditis, especially the isolation of streptococcus suis.bacterial isolation from slaughtered pigs with endocarditis was carried out from 1985 to 1994. a total of 495 (0.025%) out of 2,006,127 pigs were diagnosed as having endocarditis. though bacteria were significantly isolated from 399 of the 495 pigs, bacteria could not be isolated in 96 pigs (19.4%). in 11 pigs, 2 bacterial species were isolated from heart lesion. streptococcus suis was isolated from 127 cases (25.7%), streptococcus dysgalactiae from 75 (15.2%), erysipelothrix rhusiopathiae from ...19979035085
erysipelothrix rhusiopathiae septicemia in a neonate. 19979114208
meat safety consequences of implementing visual postmortem meat inspection procedures in danish slaughter pigs.the consequences of a change from a traditional meat inspection procedure, including manual handling, palpation and incision, to an entirely visual postmortem meat inspection procedure in danish slaughter pigs were assessed by a comparative study of the two methods in 183,383 slaughter pigs. out of 58 lesion codes (selected with a prevalence > or = 5.5 x 10(-5)), 26 (45 per cent) were assessed either as merely aesthetic or as the healed stage of an earlier lesion and nine (15 per cent) as active ...19979160530
[erysipelothrix rhusiopathiae bacteremia after dog bite].a case of erysipeloid with bacteraemia caused by erysipelothrix rhusiopathiae (er) in a previously healthy 41-year old man is presented. the bacterium was probably introduced by the bite of a dog. he was treated successfully with penicillin v. the er bacteraemia occurred without complications of endocarditis.19979235737
fatal erysipelothrix rhusiopathiae septicemia in a captive pacific white-sided dolphin (lagenorhyncus obliquidens).one male of a group of seven pacific white-sided dolphins (lagenorhynchus obliquidens) died after a brief period of nonspecific clinical signs. four beluga whales (delphinapterus leucas) and four harbor seals (phoca vitulina) were managed in the same water system. gross examination of the dolphin revealed only moderately enlarged mesenteric lymph nodes. histopathology revealed small to massive numbers of gram-positive bacilli, usually intravascular, in all tissues. bacteria were both extracellul ...19979523647
typing of staphylococcus aureus isolated from nasal carriers.eighteen staphylococcus aureus strains (9 pairs) were isolated from 9 apparently healthy persons (including 5 persons from medical staff) regarded as persistent nasal carriers in a 5-7-day or 3-5-month period, respectively. the 18 s. aureus strains were characterized phenotypically and by genotypic methods. biochemical properties determined with a commercial identification system, production of haemolysins as well as most of the antibiotic resistance date revealed an identity between both strain ...19979440205
[erysipelothrix rhusiopathiae bacteremia in a child with acute leukemia].the systemic infections caused by e. rhusiopathiae and reported in literature are very rare, majority evolving with endocarditis. the authors present a child with acute leukemia, to whom the blood culture during a high temperature episode allows to isolate this bacterium, without valvular lesions. the case is interesting because of the arisen bacteriological diagnosis problems and because of the pathogenic aspects of the infection. an animal source contact being absent, we took in account the in ...199710756759
comparison of the protein patterns of erysipelothrix rhusiopathiae strains by sds-page and autoradiography.the proteins of 12 erysipelothrix rhusiopathiae strains were radiolabelled with l-[35s] methionine, and the protein fractions were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and autoradiography. the strains were found to differ from each other in their protein patterns; thus, it was concluded that the method applied could provide useful data for the identification of strains. the described procedure was used for determining the percentage contents of protein ...19979557319
molecular cloning and analysis of a hsp (heat shock protein)-like 42 kda antigen gene of mycoplasma hyopneumoniae.the recombinant clone expressing the 42 kda protein (p42) of mycoplasma hyopneumoniae in escherichia coli was analyzed. the 4.4 kb hindiii-xmal dna fragment expressing the p42 gene product encodes three orfs: p42 and p16 in the forwarding strand, p24 in the reverse strand. sequence comparisons revealed that p42 could be part of a p65 gene, and has 62% identities with mycoplasma genitalium hsp70 gene and 56% identities with bacillus subtilis dnak gene; p16 and p24 genes share 73% and 47% identiti ...19979111943
erysipelothrix rhusiopathiae: an uncommon but ever present zoonosis.erysipelothrix rhusiopathiae is an important animal pathogen with a worldwide distribution, yet this zoonotic infection is rarely reported in humans. three cases of e. rhusiopathiae infection, which illustrate the varied clinical presentations of this pathogen in humans, are presented together with the pathological findings and treatment regimens.19989839315
protective effect of naoh-extracted erysipelothrix rhusiopathiae vaccine in pigs.a vaccine was prepared from a naoh-extracted antigen of the kyoto strain (serovar 2) of erysipelothrix rhusiopathiae (e. rhusiopathiae) with an oil adjuvant, and was injected twice at 3-week intervals into spf pigs and conventional pigs with maternal antibodies. after the second vaccination, igg-ga titers of immunized spf pigs were more than 256-fold at 3 weeks, and immunized pigs with maternal antibodies were 64-fold at 7 weeks. the pig with maternal antibodies vaccinated once with live vaccine ...19989492354
acute nonfatal erysipelas in sows in a commercial farrow-to-finish operation.acute nonfatal erysipelas was diagnosed in 4 sows in a 1,000-sow commercial farrow-to-finish operation in indiana. sows were pyrexic, lethargic, lame, and had multiple, 1.3- to 7.6-cm, erythematous rhomboid skin lesions. outbreak was attributed to failure to properly vaccinate pigs. further morbidity and mortality were prevented by treatment of clinically affected sows and all pigs in close proximity with procaine penicillin g daily for 3 consecutive days and vaccination of all pigs with questio ...19989524646
in vitro susceptibilities of aerobic and facultative non-spore-forming gram-positive bacilli to hmr 3647 (ru 66647) and 14 other antimicrobials.the comparative in vitro activity of the ketolide hmr 3647 (ru 66647) and those of structurally related macrolide-lincosamide-streptogramin compounds (erythromycin, roxithromycin, azithromycin, clarithromycin, josamycin, lincomycin, pristinamycin, and quinupristin-dalfopristin) as well as those of benzylpenicillin, doxycycline, vancomycin, teicoplanin, levofloxacin, and rifapentine against 247 aerobic and facultative non-spore-forming gram-positive bacilli were determined by an agar dilution met ...19989593121
superoxide release in human fibroblasts upon treatment with culture supernatants of the arthritogenic bacteria erysipelothrix rhusiopathiae and mycoplasma arthritidis.culture supernatants of the arthritogenic bacteria mycoplasma pneumonia, mycoplasma arthritidis, borrelia burgdorferi and erysipelothrix rhusiopathiae stimulated primary cultures of human fibroblasts to release reactive oxygen species into the environment, whereas cell walls and membranes of these bacteria had no effects. lipopolysaccharides of various gram-negative bacteria and lipid a, the lipid moiety of endotoxines, also failed to stimulate the release of reactive oxygen species by fibroblas ...19989618939
construction and vaccine potential of acapsular mutants of erysipelothrix rhusiopathiae: use of excision of tn916 to inactivate a target gene.we previously showed that acapsular transposon tn916 mutants of erysipelothrix rhusiopathiae are avirulent for mice. in this study, we constructed nonreverting acapsular mutants and examined the vaccine potential of the mutants in mice. a representative acapsular transposon mutant, 33h6, was plated on selective agar containing autoclaved chlortetracycline and quinaldic acid, and two tetracycline-sensitive mutants were obtained. sequence analysis of chromosomal regions of the mutants in which tn9 ...19989632592
occurrence of erysipelothrix spp. in broiler chickens at an abattoir.from september 1995 to august 1996, 750 chickens from 66 farms sent to an abattoir in nagano prefecture, japan, were examined for the presence of erysipelothrix spp. erysipelothrix spp. were isolated from 118 (15.7%) of 750 skin samples, 27 (7.3%) of 372 hypoderm samples, 12 (1.9%) of 630 throat samples, 106 (59.2%) of 179 feather samples, and none of 257 spleen samples. of 66 farms, 55 farms (83.3%) sent erysipelothrix-positive chickens and 11 farms (16.7%) only negative ones. of 297 erysipelot ...19989678179
cross protection against erysipelothrix rhusiopathiae serotype 10 induced by a serotype 1 and 2 vaccine. 19989682422
comparison of the protein composition of erysipelothrix rhusiopathiae strains of subtype 1a isolated from ducks and pigs.erysipelothrix rhusiopathiae strains isolated from duck carcasses and pigs were examined by sodium dodecyl sulphate--polyacrylamide gel electrophoresis (sds-page) and the results were evaluated by a computerised method in order to compare the strains for protein composition according to molecular mass. to characterise the similarity of the strains, pearson's correlation coefficient was calculated. the degree of similarity between duck strains originating from the same flock was 980-991, while th ...19989704524
properties of repeat domain found in a novel protective antigen, spaa, of erysipelothrix rhusiopathiae.erysipelothrix rhusiopathiae is a small gram-positive rod bacterium that causes erysipelas in swine and a variety of diseases in other animals and humans. although live-attenuated or bacterin vaccines are effective in protecting against erysipelas, the genetic construction of their active antigen has not been identified. to clarify the surface antigen(s) involved in protective and arthritic response, using monoclonal antibody i2a against the surface proteins of e. rhusiopathiae, we identified a ...19989712689
an outbreak of urticarial form of swine erysipelas in a medium-scale piggery in kiambu district, kenya.this report concerns an outbreak that occurred during july/august 1997. ten pigs from a herd of 181 pigs in a medium-scale, semi-closed piggery in kiambu district, kenya, contracted the clinical disease. the main clinical findings in affected pigs included: fever (40.5-41.8 degrees c), prostration, inappetence, dog-sitting posture, abortion, erythema and raised, firm to the touch and easily palpated light pink to dark purple diamond-shaped to square/rectangular spots on the skin around the belly ...19989760400
effects of tamoxifen, melatonin, coenzyme q10, and l-carnitine supplementation on bacterial growth in the presence of mycotoxins.the involvement of toxic oxygen intermediates in the bacteriostatic effects of mycotoxins (t-2 toxin, deoxynivalenol, ochratoxin a, aflatoxin b1, and fumonisin b1) was investigated by producing bacterial growth curves using turbidimetry assays in the presence and absence of oxygen radical-scavenging substances. the strains used in this study included escherichia coli (ft 101), streptococcus agalactiae (ft 311, ft 313, ft 315), staphylococcus aureus (ft 192), yersinia enterocolitica (ft 430), sal ...19989774492
use of the protective antigen of erysipelothrix rhusiopathiae in the enzyme-linked immunosorbent assay and latex agglutination.to establish a safe and convenient serodiagnostic method for swine erysipelas, a purified protective protein antigen of erysipelothrix rhusiopathiae, which included a large amount of protective protein (64 kda protein), was used for enzyme-linked immunosorbent assay (elisa) and the latex agglutination (la) test. in the elisa, the antisera to four different serovars (1a, 2, 5 and 20) of e. rhusiopathiae exhibit a positive reaction, while antisera to other species of bacteria (listeria monocytogen ...19989780828
isolation of acriflavine resistant erysipelothrix rhusiopathiae from slaughter pigs in japan.erysipelothrix rhusiopathiae is the causative agent of swine erysipelas. although an attenuated vaccine is used in japan, recent increases in disease occurrence have cast doubts on its efficacy. we investigated the similarity between the vaccine strain and e. rhusiopathiae field isolates by the analysis of acriflavine resistance (the vaccine strain marker), serotype, dna fingerprinting and pathogenicity to mice. although 7 acriflavine resistant e. rhusiopathiae isolates were separated from arthr ...19989795903
measurement of antibacterial activities of t-2 toxin, deoxynivalenol, ochratoxin a, aflatoxin b1 and fumonisin b1 using microtitration tray-based turbidimetric techniques.various mycotoxins were tested for their antibacterial activity by evaluating growth delays using a fully automated microturbidmetric method. ten different strains of the genera escherichia, streptococcus, staphylococcus, yersinia, salmonella, erysipelothrix and lactobacillus were used as test micro-organisms. t-2 toxin, deoxynivalenol (don), ochratoxin a (ota), aflatoxin b1 (afb1) and fumonisin b1 (fb1) were used as representative mycotoxins. the inhibitory effect in vitro was defined as the di ...19989838857
computerized evaluation procedure for comparing the electrophoretic protein patterns of bacterial strains.the advantages and drawbacks of different methods of evaluating electrophoretic (sds-page) protein patterns were studied by comparing erysipelothrix rhusiopathiae strains. the computerized evaluation procedure recommended by the authors renders it possible to determine objectively the degree of similarity of the protein composition of the different strains according to molecular mass. the degree of similarity obtained in four parallel analyses of a given strain was 988 +/- 0.55 on a scale on whi ...19989867344
use of an enrichment broth cultivation-pcr combination assay for rapid diagnosis of swine erysipelas.we have previously described the creation by tn916 mutagenesis of avirulent transposition mutants from a highly virulent strain of erysipelothrix rhusiopathiae, the causative agent of swine erysipelas. in this study, we cloned a 2.2-kb dna fragment which flanked the tn916 insertion in an avirulent mutant (strain 33h6) and evaluated the possibility that this region could be used for the specific detection of e. rhusiopathiae. according to the sequences of this region, oligonucleotide primers were ...19989431926
synovial fluid isolation of erysipelothrix rhusiopathiae in knee septic arthritis. 199819078295
bacteriologic analysis of infected dog and cat bites. emergency medicine animal bite infection study group.to define better the bacteria responsible for infections of dog and cat bites, we conducted a prospective study at 18 emergency departments. to be eligible for enrollment, patients had to meet one of three major criteria for infection of a bite wound (fever, abscess, and lymphangitis) or four of five minor criteria (wound-associated erythema, tenderness at the wound site, swelling at the site, purulent drainage, and leukocytosis). wound specimens were cultured for aerobic and anaerobic bacteria ...19999887159
immunological characterization of a protective antigen of erysipelothrix rhusiopathiae: identification of the region responsible for protective immunity.the gene encoding a protective protein antigen of the gram-positive bacterium erysipelothrix rhusiopathiae, an important veterinary pathogen responsible for erysipelas in swine and a variety of diseases in animals, was cloned and sequenced. the gene encodes a polypeptide of 597 amino acids plus a putative signal sequence of 29 amino acids, resulting in a mature protein with a molecular mass of 69,017 da. sequence analysis of the gene product revealed a c-terminal region composed of nine tandem r ...199910084998
isolation and purification of a protective protein antigen of erysipelothrix rhusiopathiae.the rapid growth and high survival rate of erysipelothrix rhusiopathiae was determined using a culture of the bacterium in tryptic soy broth supplemented with 0.3% tris-hydroxymethyl aminomethane and 0.1% tween 80 (tt-ts broth). high concentrations of 64, 66 and 43 kda proteins, which are associated with protection against e. rhusiopathiae infection in mice, were obtained by alkaline treatment of whole cells using 0.05-1 n naoh. the supernatant of alkaline treated cells (alkaline extract; ae) wa ...199910216450
preparation and partial characterization of monoclonal antibodies against the protective protein antigen of erysipelothrix rhusiopathiae.thirteen mouse monoclonal antibodies (mabs) against the protective protein antigen (p64) of erysipelothrix rhusiopathiae were prepared and partially characterized. the titres of the mabs varied from 200 to 1,638,400 as determined by enzyme-linked immunosorbent assay (elisa). of the 13 mabs 10, two and one belonged to the igg2a, igg1 and igm subclasses, respectively. all mabs reacted strongly with the 64 kda protein and weakly with the 43 kda protein upon western blotting of the alkaline extract ...199910216451
[comparative investigations of a combined vaccine against parvovirus and erysipelas and corresponding monovaccines in different vaccination schedules. 1: field trial].in a field trial, the development of antibodies of a combined vaccine against the porcine parvovirus (ppv) as well as against swine erysipelas was compared with corresponding mono vaccines. furthermore, these vaccines were used in different vaccination schedules. the tests were carried out on 109 gilts in three closed farms. in all gilts, a basic immunization repeated twice was carried out at the age of six months and at intervals of three weeks. the revaccination was carried out four months aft ...199910384706
erysipelas in a free-ranging hawaiian crow (corvus hawaiiensis).we describe a case of erysipelas in a free-ranging endangered hawaiian crow. the partially scavenged carcass exhibited gross emaciation and petechial hemorrhages in both lungs. microscopy revealed multiple necrotic foci associated with gram-positive rods in the liver and adrenal, diffuse acute proximal tubular necrosis of kidney, diffuse necrosis and inflammation of proventricular mucosa associated with gram-positive rods, and multiple intravascular aggregates of gram-positive rods associated wi ...199910396650
a novel protein of erysipelothrix rhusiopathiae that confers haemolytic activity on escherichia coli.erysipelothrix rhusiopathiae, the cause of swine erysipelas and human erysipeloid, produces a haemolysin. a recombinant plasmid, phly, conferring haemolytic activity on escherichia coli was isolated from a genomic library of ery. rhusiopathiae strain tama-96. this plasmid was stable in reca- e. coli, but unstable in a reca+ strain. a spontaneous deletion plasmid, pmini-hly, also conferring haemolytic activity was derived from phly. two orfs were detected in phly. analysis of pmini-hly and other ...199910411263
home intravenous antibiotic therapy for patients with infective endocarditis.although home intravenous antibiotic therapy (hiat) is increasingly being used for various infectious diseases, outpatient treatment of infective endocarditis (ie) is still uncommon. recently, the american heart association recommended outpatient treatment of endocarditis only for infections with streptococci that are highly susceptible to penicillin. herein, the experience with hiat in patients with ie due to a diversity of pathogens is presented. during a 3-year period, 37 patients with ie who ...199910421039
truncated surface protective antigen (spaa) of erysipelothrix rhusiopathiae serotype 1a elicits protection against challenge with serotypes 1a and 2b in pigs.erysipelothrix rhusiopathiae is a causal agent of swine erysipelas, which is of economic importance in the swine industry by virtue of causing acute septicemia, chronic arthritis, and endocarditis. however, little is known about the genetic properties of its protective antigens. recently, a surface protective antigen (spaa) gene was identified from serotype 2 in a mouse model. we cloned spaa from virulent strain fujisawa (serotype 1a) and determined that the n-terminal 342 amino acids without c- ...199910456877
erysipelothrix rhusiopathiae: bacteriology, epidemiology and clinical manifestations of an occupational pathogen.erysipelothrix rhusiopathiae has been recognised as a cause of infection in animals and man since the late 1880s. it is the aetiological agent of swine erysipelas, and also causes economically important diseases in turkeys, chickens, ducks and emus, and other farmed animals such as sheep. the organism has the ability to persist for long periods in the environment and survive in marine locations. infection in man is occupationally related, occurring principally as a result of contact with animals ...199910482289
valvular and myocardial abscesses due to erysipelothrix rhusiopathiae. 199910525004
serotyping and pathogenicity of erysipelothrix strains isolated from tonsils of slaughter pigs in thailand.erysipelothrix strains were isolated from the tonsils of 46 (15.0%) of 307 apparently healthy slaughter pigs in thailand during the period of august to september, 1997. a total of 27 of the 46 erysipelothrix isolates could be classified into 5 serovars but the remaining 19 were untypable in this study. of the 25 isolates serologically identified as erysipelothrix rhusiopathiae, 20, 4, and 1 isolates belonged to serovars 2, 12, and 17, respectively. only 2 isolates from the tonsils belonged to er ...199910535518
direct and rapid detection by pcr of erysipelothrix sp. dnas prepared from bacterial strains and animal tissues.a pcr method for rapid screening of erysipelothrix spp. in the slaughterhouse was carried out by using four species-specific sets of oligonucleotide primers after initial amplification with the primer set mo101-mo102, which amplifies the 16s rrna sequences of all four erysipelothrix species. the dna sequences coding for the rrna gene cluster, including 16s rrna, 23s rrna, and the noncoding region downstream of 5s rrna, were determined in order to design primers for the species-specific pcr detec ...199910565937
serovar, pathogenicity and antimicrobial susceptibility of erysipelothrix rhusiopathiae isolates from farmed wild boars (sus scrofa) affected with septicemic erysipelas in japan.six strains of erysipelothrix rhusiopathiae were isolated from farmed wild boars with acute septicemic erysipelas during the period from 1983 to 1998 in japan. all isolates belonged to serovar 1a or 2 (predominant serovars in swine). the 50 per cent lethal dose values of those isolates ranged from 10(1.3)to 10(6.2)colony forming units in mice. in swine, all isolates were virulent, capable of inducing localized or generalized urticarial lesions after intradermal inoculation. all of the isolates w ...199910607512
Displaying items 501 - 600 of 869