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identification and application of aflp-derived genetic markers for quantitative pcr-based tracking of bacillus and paenibacillus spp. released in soil.in this study, we show that noncoding sequences from amplified fragment length polymorphisms (aflps) can provide robust and sensitive genetic markers suitable for pcr-based discrimination of closely related strains of bacillus and paenibacillus, and quantitative pcr (qpcr)-based tracking of the strains in complex natural systems like soil. quantitative pcr was accurate in the approximately 1 x 10(9) to approximately 1 x 10(4) colony forming units (cfu)/g soil range. the detection limit was impro ...200919935889
bacillus amyloliquefaciens ga1 as a source of potent antibiotics and other secondary metabolites for biocontrol of plant pathogens.phytopathogenic fungi affecting crop and post-harvested vegetables are a major threat to food production and food storage. to face these drawbacks, producers have become increasingly dependent on agrochemicals. however, intensive use of these compounds has led to the emergence of pathogen resistance and severe negative environmental impacts. there are also a number of plant diseases for which chemical solutions are ineffective or non-existent as well as an increasing demand by consumers for pest ...200919941639
antifungal activity of selected indigenous pseudomonas and bacillus from the soybean rhizosphere.the purpose of this study was to isolate and select indigenous soil pseudomonas and bacillus bacteria capable of developing multiple mechanisms of action related to the biocontrol of phytopathogenic fungi affecting soybean crops. the screening procedure consisted of antagonism tests against a panel of phytopathogenic fungi, taxonomic identification, detection by pcr of several genes related to antifungal activity, in vitro detection of the antifungal products, and root colonization assays. two i ...200920016811
identification of bacillus cereus in a chungkukjang that showed high anticancer effects against ags human gastric adenocarcinoma cells.anticancer effects of chungkukjang (a korean short-term fermented soy paste) were studied in human gastric adenocarcinoma cells, and bacillus strains from chungkukjang were isolated and identified. before the experiments, six different chungkukjang products (k-, m-, mn-, o-, os-, and h-chungkukjangs) were purchased from a folk village in the sunchang region, jeonbuk, republic of korea. based on sensory evaluation tests and general chemical and quality studies, k-, h-, and m-chungkukjangs were se ...200920041781
isolation and characterization of bacterial endophytes of chelidonium majus l.the aim of this study was to isolate and identify endophytic bacteria from stems of chelidonium majus l. (greater celandine) and to evaluate their antifungal properties. in total, 34 bacterial endophyte strains were isolated. the fungistatic effects of these bacteria on the growth of five moulds (alternaria alternata, chaetonium sp., paecilomyces variotti, byssochlamys fulva, aureobasidium pullulans) and one species of black yeast (exophiala mesophila) were tested. the majority of the bacterial ...200920380146
more than anticipated - production of antibiotics and other secondary metabolites by bacillus amyloliquefaciens fzb42.the genome of environmental bacillus amyloliquefaciens fzb42 harbors numerous gene clusters involved in synthesis of antifungal and antibacterial acting secondary metabolites. five gene clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, direct non-ribosomal synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide, and the iron siderophore bacillibactin. bacillomycin and fengycin were shown to act against phytopathogenic fungi in a synergistic manner. ...200918957859
a novel vector for direct cloning pcr fragments by positive selection based on the lethal barnase.a novel vector for direct pcr fragments cloning by positive selection, pbn, was constructed based on the lethal barnase from bacillus amyloliquefaciens. barnase was modified by inserting an additional insert at a pivotal ile-54 site, which could take crucial affect on protein structure and absolute activity. the lacz' expressing cassette of puc19 was replaced by the modified barnase under the nptiii promoter. this novel vector could exist in large quantities as puc19 in e. coli hosts. for the di ...200918979229
genome analysis of bacillus amyloliquefaciens fzb42 reveals its potential for biocontrol of plant pathogens.the genome of plant-associated bacillus amyloliquefaciens fzb42 harbors an array of giant gene clusters involved in synthesis of lipopeptides and polyketides with antifungal, antibacterial and nematocidal activity. five gene clusters, srf, bmy, fen, nrs, dhb, covering altogether 137 kb, were shown to direct synthesis of the cyclic lipopeptides surfactin, bacillomycin, fengycin, an unknown peptide, and the iron-siderophore bacillibactin. in addition, one gene cluster encoding enzymes involved in ...200919041913
difficidin and bacilysin produced by plant-associated bacillus amyloliquefaciens are efficient in controlling fire blight disease.representatives of bacillus amyloliquefaciens were shown to possess biocontrol activity against fire blight, a serious disease of orchard trees caused by erwinia amylovora. genome analysis of b. amyloliquefaciens fzb42 identified gene clusters responsible for synthesis of several polyketide compounds with antibacterial action. we show here that the antibacterial polyketides difficidin and to a minor extent bacillaene act efficiently against e. amylovora. surprisingly, a mutant strain blocked in ...200919061923
transcript profiling of oilseed rape (brassica napus) primed for biocontrol differentiate genes involved in microbial interactions with beneficial bacillus amyloliquefaciens from pathogenic botrytis cinerea.many microorganisms interact with plants but information is insufficient concerning requirements for plant colonization and if interactions become beneficial or detrimental. pretreatment of oilseed rape (brassica napus) with bacillus results in disease suppression upon challenge with pathogens. we have studied transcriptome effects on oilseed rape primed with the bacillus amyloliquefaciens 5113 biocontrol strain and compared that with effects of the fungal pathogen botrytis cinerea. using the cd ...200919184461
intein-mediated protein assembly in transgenic wheat: production of active barnase and acetolactate synthase from split genes.engineering traits by the assembly of non-functional gene products is a promising tool for modern plant biotechnology. in this article, we describe the establishment of male sterility and herbicide resistance in wheat (triticum aestivum) by complementing inactive precursor protein fragments through a split intein system. n- and c-terminal fragments of a barnase gene from bacillus amyloliquifaciens were fused to intein sequences from the synechocystis sp. gene dnab and delivered into the wheat ge ...200919222807
biological interactions to select biocontrol agents against toxigenic strains of aspergillus flavus and fusarium verticillioides from maize.biological control represent an alternative to the use of pesticides in crop protection. a key to progress in biological control to protect maize against fusarium verticillioides and aspergillus flavus maize pathogens are, to select in vitro, the best agent to be applied in the field. the aim of this study was to examine the antagonistic activity of bacterial and yeast isolates against f.verticillioides and a. flavus toxigenic strains. the first study showed the impact of bacillus amyloliquefaci ...200919247799
impact of two bacterial biocontrol agents on bacterial and fungal culturable groups associated with the roots of field-grown maize.to assess the impact of bacillus amyloliquefaciens and microbacterium oleovorans on bacterial and fungal groups associated to the roots of field-grown maize.200919292823
influence of pressurization rate and pressure pulsing on the inactivation of bacillus amyloliquefaciens spores during pressure-assisted thermal processing.pressure-assisted thermal processing (patp) is an emerging sterilization technology in which a combination of pressure (500 to 700 mpa) and temperature (90 to 120 degrees c) are used to inactivate bacterial spores. the objective of this study was to examine the role of pressurization rate and pressure pulsing in enhancing patp lethality to the bacterial spore. bacillus amyloliquefaciens tmw 2.479 spore suspensions were prepared in deionized water at three inoculum levels (1.1 x 10(9), 1.4 x 10(8 ...200919435226
interventions for control of salmonella: clearance of microbial growth from rubber picker fingers.a study was conducted to determine if a surface material with antimicrobial properties combined with an effective disinfectant could achieve total clearance of bacterial contamination. before beginning the project, new rubber picker fingers collected from 3 processing facilities were tested for endogenous microflora. five species of bacteria common to soil and human handling were present: bacillus amyloliquefaciens, bacillus cereus/thuringiensis, staphylococcus epidermidis, staphylococcus homini ...200919439642
plant growth-promoting rhizobacteria allow reduced application rates of chemical fertilizers.the search for microorganisms that improve soil fertility and enhance plant nutrition has continued to attract attention due to the increasing cost of fertilizers and some of their negative environmental impacts. the objectives of this greenhouse study with tomato were to determine (1) if reduced rates of inorganic fertilizer coupled with microbial inoculants will produce plant growth, yield, and nutrient uptake levels equivalent to those with full rates of the fertilizer and (2) the minimum lev ...200919466478
studies on an antifungal protein and a chromatographically and structurally related protein isolated from the culture broth of bacillus amyloliquefaciens.an antifungal protein with multiple stable biological activities unaffected by chemical modification of tyrosine and tryptophan, and a protein with n-terminal sequence and molecular mass resemblance but no identifiable activities were isolated from culture broth of bacillus amyloliquefaciens. the findings are analogous to previous reports on thaumatin and thaumatin-like proteins.200919508197
cody activates transcription of a small rna in bacillus subtilis.regulatory small rnas (srnas) in bacterial genomes have become a focus of research over the past 8 years. whereas more than 100 such srnas have been found in escherichia coli, relatively little is known about srnas in gram-positive bacteria. using a computational approach, we identified two srnas in intergenic regions of the bacillus subtilis genome, sr1 and sr2 (renamed bsrf). recently, we demonstrated that sr1 inhibits the translation initiation of the transcriptional activator ahrc. here, we ...200919542274
[optimization of cloning and expression of beta-glucanase gene from bacillus amyloliquefaciens].to compare of performance of beta-1,3-1,4-glucanase gene (bgl) in different expression systems, the beta-1,3-1,4-glucanase gene (genbank accession no. eu623974) was amplified from bacillus amyloliquefaciens bs5582 by pcr and was cloned into three vectors pegx-4t-1, pet20b(+) and pet28a(+) to construct pegx-4t-1-bgl, pet20b(+)-bgl and pet28a(+)-bgl recombinant plasmids. the pegx-4t-1-bgl was transformed into three different escherichia coli host strains. the pet20b (+)-bgl and pet28a (+)-bgl were ...200919637629
fusion of barnase to antiferritin antibody f11 vh domain results in a partially folded functionally active protein.a chimeric protein, vh-barnase, was obtained by fusing the vh domain of anti-human ferritin monoclonal antibody f11 to barnase, a bacterial rnase from bacillus amyloliquefaciens. after refolding from inclusion bodies, the fusion protein formed insoluble aggregates. off-pathway aggregation was significantly reduced by adding either purified groel/groes chaperones or arginine, with 10-12-fold increase in the yield of the soluble protein. the final protein conformation was identical by calorimetric ...200919645673
crystallization and preliminary x-ray analysis of the lov domain of the blue-light receptor ytva from bacillus amyloliquefaciens fzb42.light-oxygen-voltage (lov) proteins play an important role in blue-light-dependent physiological processes in many organisms. the lov domain of the blue-light receptor ytva from bacillus amyloliquefaciens fzb42 has been purified and crystallized at 277 k using the sitting-drop vapour-diffusion method with 2-ethoxyethanol as a precipitant. a data set was collected to 1.60 a resolution from a single crystal at 100 k using synchrotron radiation. the lov domain of ytva crystallized in space group c2 ...200919652358
biochemical characterization of raw-starch-digesting alpha amylase purified from bacillus amyloliquefaciens.alpha amylase (e.c. 3.2.1.1) of bacillus amyloliquefaciens produced by submerged fermentation was purified to near homogeneity by ion exchange chromatography. through the process 38.6-fold increase in purity with a specific activity of 72 u/mg proteins was obtained. the apparent molecular weight of the purified enzyme was found to be 58 kda by sds-page. the enzyme was relatively stable between ph 5.0-8.0 and temperature between 50 and 60 degrees c. the enzyme did not show any obligate requiremen ...200918769877
iturin a is the principal inhibitor in the biocontrol activity of bacillus amyloliquefaciens ppcb004 against postharvest fungal pathogens.a bacillus amyloliquefaciens strain, surviving epiphytically on the surface of fruit, was isolated while searching for naturally occurring biological control agents. this bacterial strain was characterized for its antifungal activity against seven selected fungal postharvest pathogens of citrus.201019674188
efficacy of pressure-assisted thermal processing, in combination with organic acids, against bacillus amyloliquefaciens spores suspended in deionized water and carrot puree.effect of organic acids (acetic, citric, and lactic; 100 mm, ph 5) on spore inactivation by pressure-assisted thermal processing (patp; 700 mpa and 105 degrees c), high pressure processing (hpp; 700 mpa, 35 degrees c), and thermal processing (tp; 105 degrees c, 0.1 mpa) was investigated. bacillus amyloliquefaciens spores were inoculated into sterile organic acid solutions to obtain a final concentration of approximately 1.3 x 10(8) cfu/ml. b. amyloliquefaciens spores were inactivated to undetect ...201020492185
characterization of two anti-fungal lipopeptides produced by bacillus amyloliquefaciens sh-b10.bacillus amyloliquefaciens sh-b10 isolated from deep-sea sediment produces two anti-fungal lipopeptides that were purified by bioactivity-guided fractionation based on acid precipitation, vacuum flash chromatography and semi-preparative hplc. the two compounds were identified by tandem q-tof mass spectroscopy as c16 fengycin a (1) and a new fengycin with an aminobutyric acid at position 6 of the peptide backbone (2). both compounds showed significant inhibitory activities against five plant fung ...201020599380
impact of osmotic/matric stress and heat shock on environmental tolerance induction of bacterial biocontrol agents against fusarium verticillioides.bacillus amyloliquefaciens and microbacterium oleovorans reduced the fusarium verticillioides count and significantly decreased fumonisin b(1) and b(2) levels in maize grains. the aim of this study was to determine the effect of water stress tolerance and heat shock survival upon cells of the biocontrol agents b. amyloliquefaciens and m. oleovorans. the a(w) of solid and liquid media and tryptic soy medium was modified to 0.99, 0.98, 0.97 and 0.96 by addition of ionic solute nacl and non-ionic s ...201020624459
the final steps of bacillaene biosynthesis in bacillus amyloliquefaciens fzb42: direct evidence for beta,gamma dehydration by a trans-acyltransferase polyketide synthase. 201020087918
structure of bacillus amyloliquefaciens alpha-amylase at high resolution: implications for thermal stability.the crystal structure of bacillus amyloliquefaciens alpha-amylase (baa) at 1.4 a resolution revealed ambiguities in the thermal adaptation of homologous proteins in this family. the final model of baa is composed of two molecules in a back-to-back orientation, which is likely to be a consequence of crystal packing. despite a high degree of identity, comparison of the structure of baa with those of other liquefying-type alpha-amylases indicated moderate discrepancies at the secondary-structural l ...201020124706
antibacterial and antilarval compounds from marine gorgonian-associated bacterium bacillus amyloliquefaciens scsio 00856. 201020150929
cloning of apre86-1 gene encoding 27 kda mature fibrinolytic enzyme from bacillus amyloliquefaciens ch86-1.a gene, encoding the major secreted fibrinolytic protein of bacillus amyloliquefaciens ch86-1, was cloned from the genomic dna. dna sequencing showed that the gene, apre86-1, could direct the synthesis of a mature protein of 275 amino acids long after processing. when apre86-1 was introduced into b. subtilis, 27 kda mature protein was produced as expected. the fibrinolytic activity of b. subtilis transformant (tf) was higher than that of b. amyloliquefaciens ch86-1, showing the possibility of in ...201020208443
role of val289 residue in the alpha-amylase of bacillus amyloliquefaciens mtcc 610: an analysis by site directed mutagenesis.the val289 residue in the alpha-amylase of bacillus amyloliquefaciens, which is equivalent to the ala289 and val286 residues in the alpha-amylases of b. stearothermophilus and b. licheniformis, respectively, was studied by site-directed mutagenesis. this residue was substituted with 10 different amino acids by random substitution of the val codon. in these mutant alpha-amylases, val289 was substituted with ile, tyr, phe, leu, gly, pro, ser, arg, glu, and asp. compared with the wild-type alpha-am ...201020372028
gamma-polyglutamic acid (gamma-pga) produced by bacillus amyloliquefaciens c06 promoting its colonization on fruit surface.bacillus amyloliquefaciens c06, an effective biological agent in controlling brown rot of stone fruit caused by monilinia fructicola, was also found to produce extra-cellular mucilage and form mucoid colonies on semi-solid surfaces. this study aimed to characterize the extra-cellular mucilage produced by b. amyloliquefaciens c06 using transposon mutagenesis and biochemical and physical analyses. the mucilage production in b. amyloliquefaciens c06 was demonstrated to be associated with ywsc gene ...201020638145
optimization of protease secretion in bacillus subtilis and bacillus licheniformis by screening of homologous and heterologous signal peptides.bacillus subtilis and bacillus licheniformis are widely used for the large-scale industrial production of proteins. these strains can efficiently secrete proteins into the culture medium using the general secretion (sec) pathway. a characteristic feature of all secreted proteins is their n-terminal signal peptides, which are recognized by the secretion machinery. here, we have studied the production of an industrially important secreted protease, namely, subtilisin bpn' from bacillus amyloliquef ...201020709850
lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway.bacillus amyloliquefaciens wh1 inhibit the growth of fungi by producing a new surfactin called as wh1fungin. wh1fungin plays an anti-fungal role by two models: high concentration to elicit pores on cell membrane and low concentration to induce apoptosis. wh1fungin can also inhibits the glucan synthase resulting in a decreased synthesis of callose on fungal cell wall. further detection revealed that classical apoptotic markers such as reactive oxygen species (ros) accumulation, phosphatidylserine ...201020713103
toxinogenic and spoilage potential of aerobic spore-formers isolated from raw milk.the harmful effects on the quality and safety of dairy products caused by aerobic spore-forming isolates obtained from raw milk were characterized. quantitative assessment showed strains of bacillus subtilis, the bacillus cereus group, paenibacillus polymyxa and bacillus amyloliquefaciens to be strongly proteolytic, along with bacillus licheniformis, bacillus pumilus and lysinibacillus fusiformis to a lesser extent. lipolytic activity could be demonstrated in strains of b. subtilis, b. pumilus a ...201019944473
hydrolysis of amylopectin by amylolytic enzymes: structural analysis of the residual amylopectin population.amylopectin fine structures were studied following limited hydrolysis of gelatinised waxy maize starch by amylases with a different level of inner chain attack (lica). this was done by size exclusion chromatography as well as by debranching the (partially hydrolysed) amylopectin samples and studying the size distributions of the released chains. alpha-amylases from bacillus amyloliquefaciens and aspergillus oryzae, with a relatively high lica, drastically altered amylopectin chain length distrib ...201019962130
hydrolysis of amylopectin by amylolytic enzymes: level of inner chain attack as an important analytical differentiation criterion.differences in amylase action pattern on amylopectin were demonstrated by the relation between the decrease in potassium iodide-iodine binding of waxy maize starch and the increase in reducing value during hydrolysis, as expressed by the rv(80) value (i.e., the reducing value for a potassium iodide-iodine binding value of 80% of that of the starting material). in the initial stages of the hydrolysis, the ratio of the increase in the level of reducing polysaccharides to the increase in the total ...201019962134
bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil.a gram-positive bacterium, designated strain cbmb205(t), was isolated from the rhizosphere soil of traditionally cultivated, field-grown rice. cells were strictly aerobic, motile, rod-shaped and formed endospores. the best growth was achieved at 30°c and ph 7.0 in ammonium mineral salts (ams) medium containing 600 mm methanol. a comparative 16s rrna gene sequence-based phylogenetic analysis placed strain cbmb205(t) in a clade with the species bacillus amyloliquefaciens, bacillus vallismortis, ba ...201019966000
physical and biological treatments of polyethylene-rice starch plastic films.this study aimed to produce an industrial applicable thermo-stable alpha-amylase from marine bacillus amyloliquefaciens which isolated and selected according to its significant enzyme production. the effect of different ph values and temperatures on the bacterial growth and the enzyme production was estimated using an experimental statistical design; maximum amylase production and bacterial growth was obtained at ph 7.0 and 50 degrees c. some biodegradable polyethylene rice starch plastic films ...201020006438
gene cloning, characterization, and heterologous expression of levansucrase from bacillus amyloliquefaciens.although levan produced by bacillus amyloliquefaciens is known to have efficient immunostimulant property which gives 100% survival of common carp when infected with aeromonas hydrophila, no detailed reports are available describing kinetic studies of d: -glucose production and levan formation. in this study, we cloned and characterized the enzymatic kinetics using levansucrase expressed in escherichia coli. optimum ph for d: -glucose production and levan formation was 6.0 and 8.0, respectively, ...201019916084
bacillus siamensis sp. nov., isolated from salted crab (poo-khem) in thailand.a gram-positive, endospore-forming, rod-shaped bacterium, strain pd-a10(t), was isolated from salted crab (poo-khem) in thailand and subjected to a taxonomic study. phenotypic and chemotaxonomic characteristics, including phylogenetic analyses, showed that the novel strain was a member of the genus bacillus. the novel strain grew in medium with 0-14 % (w/v) nacl, at 4-55°c and at ph4.5-9. the predominant quinone was a menaquinone with seven isoprene units (mk-7). the major fatty acids were antei ...201019933584
a simple method to introduce marker-free genetic modifications into the chromosome of naturally nontransformable bacillus amyloliquefaciens strains.a simple method to introduce marker-free deletions, insertions, and point mutations into the chromosomes of naturally nontransformable bacillus amyloliquefaciens strains has been developed. the method is efficient and fast, and it allows for the generation of genetic modifications without the use of a counter-selectable marker or a special prerequisite strain. this method uses the combination of the following: the effective introduction of a delivery plasmid into cells for gene replacement; a tw ...201019820923
role of the calcium-binding residues asp231, asp233, and asp438 in alpha-amylase of bacillus amyloliquefaciens as revealed by mutational analysis.role of the calcium-binding residues asp231, asp233, and asp438 of bacillus amyloliquefaciens alpha-amylase (baa) on the enzyme properties was investigated by site-directed mutagenesis. the calcium-binding residues asp231, asp233, and asp438 were replaced with asn, asn, and gly to produce the mutants d231n, d233n, and d438g, respectively. the mutant amylases were purified to homogeneity and the purified enzymes was estimated to be approximately 58 kda. the specific activity for the mutant enzyme ...201019841977
fibrinolytic serine protease isolation from bacillus amyloliquefaciens an6 grown on mirabilis jalapa tuber powders.in this study, mirabilis jalapa tuber powder (mjtp) was used as a new complex organic substrate for the growth and production of fibrinolytic enzymes by a newly isolated bacillus amyloliquefaciens an6. maximum protease activity (1,057 u/ml) with casein as a substrate was obtained when the strain was grown in medium containing (grams per liter) mjtp 30, yeast extract 6, cacl(2) 1, k(2)hpo(4) 0.1, and k(2)hpo(4) 0.1. the strain was also found to grow and produce extracellular proteases in a medium ...201019842068
relationship of bacillus amyloliquefaciens clades associated with strains dsm7t and fzb42: a proposal for bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discriminating complete genome sequences.the whole genome sequenced rhizobacterium fzb42 (chen et al., 2007) and other plant-associated bacillus strains either designated as bacillus amyloliquefaciens or bacillus subtilis are used commercially to promote growth and health of crop plants. previous investigations revealed that the strains represent an own ecotype related to b. amyloliquefaciens, however its exact taxonomic position remains elusive (reva et al., 2004). here we have demonstrated ability to colonize arabidopsis roots for a ...201020817842
effect of ionic liquids on the structure, stability and activity of two related α-amylases.ionic liquids are recognized as green solvents for carbohydrates dissolution. however, only a limited number of studies have been carried out to investigate their effect on carbohydrate hydrolyzing enzymes. we have investigated the influence of two water miscible ionic liquids on the activity, stability and structure of two related α-amylases from bacillus amyloliquefaciens and bacillus lichiniformis. upon changes in ionic liquids concentrations, both enzymes activity and stability were reduced. ...201020946913
enhancing electro-transformation competency of recalcitrant bacillus amyloliquefaciens by combining cell-wall weakening and cell-membrane fluidity disturbing.bacillus amyloliquefaciens has been a major workhorse for the production of a variety of commercially important enzymes and metabolites for the past decades. some subspecies of this bacterium are recalcitrant to exogenous dna, and transformation with plasmid dna is usually less efficient, thereby limiting the genetic manipulation of the recalcitrant species. in this work, a methodology based on electro-transformation has been developed, in which the cells were grown in a semicomplex hypertonic m ...201020951110
bacteriocin-like substance from bacillus amyloliquefaciens shows remarkable inhibition of acanthamoeba polyphaga.the effectiveness of a bacteriocin-like substance (bls) produced by bacillus amyloliquefaciens was tested against acanthamoeba polyphaga strains, and its cytotoxic potential on vero cells was investigated. amebicidal activity of the purified bls was tested by plate bioassays with concentrations ranging from 12.5 to 6,400 au ml(-1). damage to a. pholyphaga cells was monitored using an inverted microscope and counted in a fuchs-rosenthal chamber after 24, 48, and 72 h. according to the results obt ...201020967460
plantazolicin, a novel microcin b17/streptolysin s-like natural product from bacillus amyloliquefaciens fzb42.here we report on a novel thiazole/oxazole-modified microcin (tomm) from bacillus amyloliquefaciens fzb42, a gram-positive soil bacterium. this organism is well known for stimulating plant growth and biosynthesizing complex small molecules that suppress the growth of bacterial and fungal plant pathogens. like microcin b17 and streptolysin s, the tomm from b. amyloliquefaciens fzb42 undergoes extensive posttranslational modification to become a bioactive natural product. our data show that the mo ...201020971906
immobilization of bacillus amyloliquefaciens mbl27 cells for enhanced antimicrobial protein production using calcium alginate beads.cell immobilization is one of the common techniques for increasing the overall cell concentration and productivity. bacillus amyloliquefaciens mbl27 cells were immobilized in calcium alginate beads and it is a promising method for repeated amp (antimicrobial protein) production. the present study aimed at determining the optimal conditions for immobilization of b. amyloliquefaciens mbl27 cells in calcium alginate beads and the operational stability for enhanced production of the amp. amp product ...201021044046
comparison of microbial diversity of edible flowers and basil grown with organic versus conventional methods.the consumption and use of edible flowers as food is growing; however, no study has been conducted to evaluate their role in the cause of food-borne illness or in food safety. recent food-borne outbreaks traced to fresh herbs have raised concern about their processing and handling. basil, one of the most commonly used fresh herbs, has been identified as a source of food-borne illness. baseline assessments of microflora were performed, and the microbial diversity between growing methods (organic ...201021076485
antimicrobial activity of bacillus amyloliquefaciens lbm 5006 is enhanced in the presence of escherichia coli.increased antimicrobial activity was observed when bacillus amyloliquefaciens lbm 5006 strain was cultivated in the presence of thermally inactivated cells of escherichia coli, but not with staphylococcus aureus, listeria monocytogenes, or bacillus cereus. e. coli also enhanced the antimicrobial activity when it was added to the medium in the form of living cells or as cell debris after cellular fractionation. no inducing activity was observed with addition of cell-free supernatant of e. coli cu ...201021104084
functional analysis and application of the cryptic plasmid pbsg3 harboring the rapq-phrq system in bacillus amyloliquefaciens b3.this work sequenced and characterized a cryptic plasmid called pbsg3 from wild-type bacillus amyloliquefaciens b3--a powerful agent for suppression of plant pathogenic organisms. it is an 8439 bp circular molecule, with g+c content of 40.3%. we provide evidence that pbsg3 replicates via the rolling-circle (rc) mechanism and, sequence comparisons place it in the pc194 family of rolling-circle-replicons. the plasmid contains seven putative open reading frames (orfs), including genes repb3, mobb3, ...201021118702
[improvement of thermostability of beta-1,3-1,4-glucanase from bacillus amyloliquefaciens bs5582 through in vitro evolution].in vitro evolution methods are often used to modify protein with improved characteristics. we developed a directed evolution protocol to enhance the thermostability of the beta-1,3-1,4-glucanase. the thermostability of the enzyme was significantly improved after two rounds of directed evolution. three variants with higher thermostability were obtained. the mutant enzymes were further analyzed by their melting temperature, halftime and kinetic parameters. comparing to intact enzyme, the t50 of mu ...201021141122
novel starter cultures to inhibit biogenic amines accumulation during fish sauce fermentation.bacteria with amine oxidase activity have become a particular interest to reduce biogenic amines concentration in food products such as meat and fish sausages. however, little information is available regarding the application of these bacteria in fish sauce. hence, our study was aimed to investigate the effect of such starter cultures in reducing biogenic amines accumulation during fish sauce fermentation. staphylococcus carnosus fs19 and bacillus amyloliquefaciens fs05 isolated from fish sauce ...201021183239
inactivation of bacillus amyloliquefaciens spores by a combination of sucrose laurate and pressure-assisted thermal processing.the aim of this research was to study the effect of sucrose laurate ester (sl) on enhancing pressure-assisted thermal processing (patp) inactivation of bacillus amyloliquefaciens fad 82 spores. b. amyloliquefaciens spores (∼10⁸ cfu/ml) were suspended in deionized water, solutions of 0.1, 0.5, and 1.0% sl, and mashed carrots without or with 1% sl. samples were treated at 700 mpa and 105°c for 0 (come-up time), 1, 2, and 5 min and analyzed by pour-plating and most-probable-number techniques. heat ...201021219716
isolation and characterization of antifungal peptides produced by bacillus amyloliquefaciens lbm5006.bacillus amyloliquefaciens lbm 5006 produces antagonistic activity against pathogenic bacteria and phytopathogenic fungi, including aspergillus spp., fusarium spp., and bipolaris sorokiniana. pcr analysis revealed the presence of itud, but not sfp genes, coding for iturin and surfactin, respectively. the antimicrobial substance produced by this strain was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction. the ultraviolet spectrum was typical of a ...201021221936
glutamic acid independent production of poly-γ-glutamic acid by bacillus amyloliquefaciens ll3 and cloning of pgsbca genes.a new glutamic acid independent poly-γ-glutamic acid (γ-pga) producing strain, which was identified as bacillus amyloliquefaciens ll3 by analysis of 16s rdna and gyrase subunit a gene (gyra), was isolated from fermented food. the product had a molecular weight of 470, 801 and l-glutamate monomer content of 98.47%. the pre-optimal medium, based on single-factor tests and orthogonal design, contained 50 g/l sucrose, 2g/l (nh(4))(2)so(4), 0.6g/l mgso(4), and provided well-balanced changes in proces ...201021232939
a new strategy for generating geminivirus resistant plants using a dna betasatellite/split barnase construct.the betasatellite dna associated with cotton leaf curl disease contains a single orf, βc1, which is a pathogenicity determinant. deletion of the βc1 orf showed that it was not required for betasatellite replication in the presence of tomato leaf curl virus-australia (tlcv-au). a series of betasatellite/split mutant barnase gene constructs, in which a direct repeat of the bacillus amyloliquefaciens barnase gene flanked the betasatellite, were shown to replicate in tobacco in the presence of tlcv- ...201020813135
antibacterial activity of subtilosin alone and combined with curcumin, poly-lysine and zinc lactate against listeria monocytogenes strains.in this paper, the antibacterial effects of the bacillus amyloliquefaciens-produced bacteriocin subtilosin, both alone and in combination with curcumin, ε-poly-l-lysine (poly-lysine), or zinc lactate, were examined against listeria monocytogenes. results indicated that subtilosin inhibits both of the studied bacterial strains, scott a (wild-type, nisin sensitive) and nr30 (nisin resistant). however, l. monocytogenes scott a was more sensitive to subtilosin and pure curcumin. in addition, subtilo ...201026781320
antilisterial activity on poultry meat of amylolysin, a bacteriocin from bacillus amyloliquefaciens ga1.this paper describes the production, the purification and the antilisterial activity of amylolysin, a novel bacteriocin from b. amyloliquefaciens ga1. the strain genome was first analysed using pcr techniques for the presence of gene clusters that direct the synthesis of characterised bacteriocins from b. amyloliquefaciens and the closely related b. subtilis. our results suggest that amylolysin corresponds to a novel bacteriocin. the effect of amylolysin on the growth of different isolates of li ...201026781121
[analysis of microorganism species diversity in plant intercropping models in a wetland system constructed for treatment of municipal sewage].the selective culture method and pcr-dgge technology were applied to analyze the number and the biodiversity of microorganism species in cells with plant intercropping models and without plants in different seasons in a wetland system constructed for treatment of municipal sewage. the results showed that the numbers of microorganisms were considerably larger in the cells with plant intercropping models than those without plants, while the number of microorganisms was apparently larger in summer ...201122619969
isolation and characterization of putative probiotic bacterial strain, bacillus amyloliquefaciens, from north east himalayan soil based on in vitro and in vivo functional properties.this study is aimed to isolate some novel probiotics from the soils of north east himalayas. eleven gram positive isolates were obtained in mrs with oxgall media from soil samples. four of the isolates withstood the in vitro gastric juice ph 3.0 and 0.45% bile salt tolerance screening. among these, pbt 3 showed high cell surface hydrophobicity and adhered to the caco-2 cells. 16s rdna gene sequences of this probiotic strains were identified as bacillus amyloliquefaciens (accession no: jf836079). ...201126781678
enhancement of extracellular purine nucleoside accumulation by bacillus strains through genetic modifications of genes involved in nucleoside export.using a simple method to introduce genetic modifications into the chromosome of naturally nontransformable bacillus, a set of marker-free inosine-producing and 5-aminoimidazole-4-carboxamide (aica) ribonucleoside-producing bacillus amyloliquefaciens strains has been constructed. these strains differ in expression levels of the genes responsible for nucleoside export. overexpression of b. amyloliquefaciens pbue and heterologous expression of escherichia coli nepi, which encode nucleoside efflux t ...201120814730
Quantification of the antifungal lipopeptide iturin A by high performance liquid chromatography coupled with aqueous two-phase extraction.Iturin A, a powerful antifungal surfactant, is a kind of bacterial lipopeptide produced by Bacillus strains. This study addresses the use of an aqueous two-phase system (ATPS) using ethanol/ammonium sulfate to extract iturin A from Bacillus amyloliquefaciens NJN-6 fermentation broth and the quantification of iturin A by HPLC. Baseline separation of iturin A homologues was performed using an RP-C(18) column with a mixture of water and acetonitrile. The results showed that the correlation coeffici ...201121872538
An analysis of temperature adaptation in cold active, mesophilic and thermophilic Bacillus a-amylases.A comparative biochemical and structural study was performed on a cold active a-amylase from Bacillus cereus (BCA) and two well-known homologous mesophilic and thermophilic a-amylases from Bacillus amyloliquefaciens (BAA) and Bacillus licheniformis (BLA). In spite of a high degree of sequence and structural similarity, drastic variations were found for T(opt) as 50, 70 and 90°C for BCA, BAA and BLA, respectively. The half-lives of thermoinactivation were 1 and 9 min for BCA and BAA at 80°C respe ...201121907234
[isolation and identification of a bacillus amyloliquefaciens yb-3 against rhizoctonia solani].an antagonistic bacterial strain yb-3 against rhizoctonia solani was isolated from soils.201122097779
Elucidation of the Molecular Mechanisms of Action of the Natural Antimicrobial Peptide Subtilosin Against the Bacterial Vaginosis-associated Pathogen Gardnerella vaginalis.Subtilosin A is a 35-amino acid long cyclical peptide produced by Bacillus amyloliquefaciens that has potent antimicrobial activity against a variety of human pathogens, including the bacterial vaginosis-related Gardnerella vaginalis. The specific mode of action of subtilosin against G. vaginalis was elucidated by studying its effects on the proton motive force's (PMF) components: transmembrane electric potential (??), transmembrane pH gradient (?pH), and intracellular ATP levels. The addition o ...201121949544
Purification and Characterization of a Major Fibrinolytic Enzyme from Bacillus amyloliquefaciens MJ5-41 Isolated from Meju.Meju is a traditional Korean fermented soy product used as a key element for soy sauce and doenjang. Bacilli with antimicrobial activity were isolated from meju prepared by traditional methods at Sunchang county, Jeollabukdo, Korea. Six isolates were identified as Bacillus amyloliquefaciens by recA gene sequencing and RAPD-PCR. One isolate, B. amyloliquefaciens MJ5-41, showed the strongest fibrinolytic activity. A 27 kDa active fibrinolytic enzyme, AprE5-41, was purified from the culture superna ...201122127128
Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499.Cyclic lipopeptides of the surfactin, iturin and fengycin families synthesized by plant-associated Bacilli represent an important class of antibiotics since they may be tightly involved in the protective effect of selected strains against phytopathogens. However, their production by Bacillus cells developing on roots under rhizosphere conditions is still poorly understood. In this work, we combined electrospray and imaging mass spectrometry-based approaches to determine the detailed pattern of s ...201122029651
Structure determination and interception of biosynthetic intermediates for the plantazolicin class of highly discriminating antibiotics.The soil-dwelling, plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42 is a prolific producer of complex natural products. Recently, a new FZB42 metabolite, plantazolicin (PZN), has been described as a member of the growing thiazole/oxazole-modified microcin (TOMM) family. TOMMs are biosynthesized from inactive, ribosomal peptides and undergo a series of cyclodehydrations, dehydrogenations, and other modifications to become bioactive natural products. Using high-resolution mass spe ...201121950656
Antimicrobial factor from Bacillus amyloliquefaciens inhibits Paenibacillus larvae, the causative agent of American foulbrood.Bacillus amyloliquefaciens LBM 5006 produces an antimicrobial factor active against Paenibacillus larvae, a major honeybee pathogen. The antagonistic effect and the mode of action of the antimicrobial factor were investigated. The antibacterial activity was produced starting at mid-logarithmic growth phase, reaching its maximum during the stationary phase. Exposure of cell suspensions of P. larvae to this antimicrobial resulted in loss of cell viability and reduction in optical density associate ...201121858429
antifungal lipopeptides from bacillus amyloliquefaciens strain bo7.three new lipopeptides (1-3) were isolated from the organic extract of bacillus amyloliquefaciens strain (bo7). these compounds represented the major constituents (>60%) of the total cell lipids extractable with chcl(3)/meoh (2:1). elucidation of their chemical structure was carried out by spectroscopic analyses, including 1d and 2d nmr spectroscopy, mass spectrometry (ms), and secondary ion mass spectrometry (ms/ms), along with chemical degradation. the compounds are members of the surfactins f ...201121235220
efficient colonization of plant roots by the plant growth promoting bacterium bacillus amyloliquefaciens fzb42, engineered to express green fluorescent protein.a single copy of the gfp gene linked with the p(spac) promoter and flanked by the terminal fzb42 amye sequences was stably integrated into the chromosome of plant growth promoting bacterium bacillus amyloliquefaciens fzb42 via homologous recombination. a spontaneous mutant, fb01mut, emitting bright fluorescence was detected among the transformants and found suitable for colonization experiments performed with zea mays, arabidopsis thaliana and lemna minor. real-time rt-pcr revealed that fb01mut ...201121237217
genome sequence of b. amyloliquefaciens type strain dsm7(t) reveals differences to plant-associated b. amyloliquefaciens fzb42.the complete genome sequence of bacillus amyloliquefaciens type strain dsm7(t) is presented. a comparative analysis between the genome sequences of the plant associated strain fzb42 (chen et al., 2007) with the genome of b. amyloliquefaciens dsm7(t) revealed obvious differences in the variable part of the genomes, whilst the core genomes were found to be very similar. the strains fzb42 and dsm7(t) have in common 3345 genes (cds) in their core genomes; whilst 547 and 344 cds were found to be uniq ...201121262282
strong and consistently synergistic inactivation of spores of spoilage-associated bacillus and geobacillus spp. by high pressure and heat compared with inactivation by heat alone.the inactivation of spores of four low-acid food spoilage organisms by high pressure thermal (hpt) and thermal-only processing was compared on the basis of equivalent thermal lethality calculated at a reference temperature of 121.1°c (f(z)(121.1)(°)(c, 0.1 mpa or 600 mpa)) and characterized as synergistic, not different or protective. in addition, the relative resistances of spores of the different spoilage microorganisms to hpt processing were compared. processing was performed and inactivation ...201121278265
expression of an endotoxin-free s-layer/allergen fusion protein in gram-positive bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy.genetic fusion of the major birch pollen allergen (bet v1) to bacterial surface-(s)-layer proteins resulted in recombinant proteins exhibiting reduced allergenicity as well as immunomodulatory capacity. thus, s-layer/allergen fusion proteins were considered as suitable carriers for new immunotherapeutical vaccines for treatment of type i hypersensitivity. up to now, endotoxin contamination of the fusion protein which occurred after isolation from the gram-negative expression host e. coli had to ...201121310062
functions of lipopeptides bacillomycin d and fengycin in antagonism of bacillus amyloliquefaciens c06 towards monilinia fructicola.in previous studies, bacillus amyloliquefaciens c06 has been proven to be effective in controlling brown rot of stone fruit caused by monilinia fructicola. when tested in vitro, cell-free filtrate of b. amyloliquefaciens c06 significantly inhibited mycelial growth and conidial germination of the fungal pathogen. this study aimed to determine the role of the antifungal compound(s) in the cell-free filtrate of b. amyloliquefaciens c06 by an approach combining a dna-based suppression subtractive hy ...201121335978
single step purification and characterization of a thermostable and calcium independent a-amylase from bacillus amyloliquifaciens tswk1-1 isolated from tulsi shyam hot spring reservoir, gujarat (india).a thermophilic bacteria, identified and designated as bacillus amyloliquifaciens tswk1-1 (16s rrna gene sequence, genbank: gq121033), was isolated from a hot water reservoir located at tulsi shyam, gujarat, india. the optimum temperature and ph for amylase production were 50 °c and 7.0, respectively. the crude enzyme was partially purified by ammonium sulphate fractionation followed by dialysis. however, single step purification was achieved on phenyl sepharose 6ff affinity column with 45.71% yi ...201121352849
characterization of bacillus subtilis hc8, a novel plant-beneficial endophytic strain from giant hogweed.thirty endophytic bacteria were isolated from various plant species growing near saint-petersburg, russia. based on a screening for various traits, including plant-beneficial properties and dna fragment patterns, potential siblings were removed. the remaining isolates were taxonomically identified using 16s rdna sequences and potential human and plant pathogens were removed. the remaining strains were tested for their ability to promote radish root growth and to protect tomato plants against tom ...201121366893
production of fibrinolytic enzyme from bacillus amyloliquefaciens by fermentation of chickpeas, with the evaluation of the anticoagulant and antioxidant properties of chickpeas.to develop safe and cheap thrombolytic agents, a fibrinolytic enzyme productive strain of lsse-62 was isolated from chinese soybean paste. this strain was identified as bacillus amyloliquefaciens by 16s rdna sequence analysis. nucleotide and amino acid sequence analysis showed that this fibrinolytic enzyme was identical to subtilisin dj-4. chickpeas were used as the substrate for fibrinolytic enzyme production from b. amyloliquefaciens in solid-state fermentation. under the optimized conditions ...201121391672
overexpression and characterization of thermostable chitinase from bacillus atrophaeus sc081 in escherichia coli.the chitinase-producing strain sc081 was isolated from korean traditional soy sauce and identified as bacillus atrophaeus based on a phylogenetic analysis of the 16s rdna sequence and a phenotypic analysis. a gene encoding chitinase from b. atrophaeus sc081 was cloned in escherichia coli and was named scchi-1 (gq360078). the scchi-1 nucleotide sequences were composed of 1788 base pairs and 596 amino acids, which were 92.6, 89.6, 89.3, and 78.9% identical to those of bacillus subtilis (abg57262), ...201121429298
synthesis of stable cadmium sulfide nanoparticles using surfactin produced by bacillus amyloliquifaciens strain ksu-109.in this study, a surfactin was extracted from a novel surfactant producing bacterial strain bacillus amyloliquifaciens ksu-109, isolated from rhizosphere of date palm (phoenix dactylifera), and characterized based on 16sr rna and sfp genes using blastn, blastx and phylogenetic analyses. the study was performed to obtain a renewable bioresource for surfactin production, and its application in nanotechnology as a non-hazardous and environmentally compatible nanoparticle (np) stabilizer. the strain ...201121435848
structures of clusters in sweetpotato amylopectin.sweetpotato amylopectin was subjected to partial hydrolysis by α-amylase from bacillus amyloliquefaciens to release the clusters. clusters were then fractionated and precipitated by methanol and structurally characterized by gel-permeation chromatography and high-performance anion-exchange chromatography. an initial stage of α-amylolysis on the amylopectin isolated mostly domains but also clusters. a second stage of α-amylolysis on the domains and clusters further isolated their respective clust ...201121507388
complete genome sequence of bacillus amyloliquefaciens ta208, a strain for industrial production of guanosine and ribavirin.here, we report the complete genome sequence of bacillus amyloliquefaciens ta208, a strain for industrial production of guanosine and synthesis of ribavirin by assimilation of formamide. comparison of its genome sequence with those of strains dsm7 and fzb42 revealed horizontal gene transfer represented by unique prophages and restriction-modification systems and indicated significant accumulation of guanosine.201121515778
production of fusarium verticillioides biocontrol agents, bacillus amyloliquefaciens and microbacterium oleovorans, using different growth media: evaluation of biomass and viability after freeze-drying.the aims of this study were to compare the viability and biomass production of b. amyloliquefaciens and m. oleovorans in different growth media, and the efficiency of a freeze-drying method as a possible formulation process. b. amyloliquefaciens and m. oleovorans were grown in 100 ml of four different media. media water activity was modified at 0.99, 0.98, 0.97 and 0.96. nutrient yeast dextrose broth (nydb) and molasses soy powder (msb) media were selected and survival levels of cells were deter ...201121547798
complete genome sequence of bacillus amyloliquefaciens ll3 with glutamic acid-independent production of poly-{gamma}-glutamic acid.bacillus amyloliquefaciens is one of most prevalent gram-positive aerobic spore-forming bacteria with the ability of synthesizing polysaccharides and polypeptides. here, we report the complete genome sequence of b. amyloliquefaciens ll3, which was isolated from fermented food and presents the glutamic acid-independent production of poly-γ-glutamic acid.201121551302
plantazolicin a and b: structure elucidation of ribosomally synthesized thiazole/oxazole peptides from bacillus amyloliquefaciens fzb42.the structures of the ribosomally synthesized peptide antibiotics from bacillus amyloliquefaciens fzb42, plantazolicin a and b, have been elucidated by high resolving esi-msms, 2d (1)h-(13)c-correlated nmr spectroscopy as well as (1)h-(15)n-hmqc/(1)h-(15)n-hmbc nmr experiments. (15)n-labeling prior to the experiments facilitated the structure determination, unveiling a hitherto unusual number of thiazoles and oxazoles formed from a linear 14mer precursor peptide. this finding further extends the ...201121568297
cloning and expression of a bpr gene encoding bacillopeptidase f from bacillus amyloliquefaciens ch86-1.a gene encoding bacillopeptidase f, bpr86-1, was cloned from b. amyloliquefaciens ch86-1 isolated from cheonggukjang. this gene could encode a preproenzyme of 1,431 amino acids. when bpr86-1 was introduced into b. subtilis wb600 via phy300plk, an e. coli-bacillus shuttle vector, the transformant showed fibrinolytic activity. during growth on lb, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. the highest activity (761.4 mu/mg protein) was observed at ...201121617349
optimization of the production of poly-╬│-glutamic acid by bacillus amyloliquefaciens c1 in solid-state fermentation using dairy manure compost and monosodium glutamate production residues as basic substrates.poly-╬│-glutamic acid (╬│-pga) is a polymer with uses in foods, cosmetics, medicine and agriculture. the medium for the production of ╬│-pga by bacillusamyloliquefaciens c1 was optimized by response surface methodology using agro-industrial wastes in solid-state fermentation (ssf). the optimal ssf medium (20g substrates with 50% initial moisture) for producing ╬│-pga was determined to contain 5.51g dairy manure compost, 1.91g soybean cake, 0.57g corn flour, 2.15g monosodium glutamate production ...201121665467
dynamics of bacterial communities during solid-state fermentation using agro-industrial wastes to produce poly-╬│-glutamic acid, revealed by real-time pcr and denaturing gradient gel electrophoresis (dgge).the dynamics of bacterial communities play an important role in solid-state fermentation (ssf). poly-╬│-glutamic acid (╬│-pga) was produced by bacillus amyloliquefaciens c1 in ssf using dairy manure compost and monosodium glutamate production residuals as basic substrates. the production of ╬│-pga reached a maximum of 0.6% after 20┬ádays fermentation. real-time polymerase chain reaction showed the amount of total bacteria reached 3.95ôçë├ùôçë10(9) 16s rdna copies/g sample after 30┬ádays, which w ...201121670980
the purl gene of bacillus subtilis is associated with nematicidal activity.parasitic nematodes of plants are important plant pathogens that represent a significant financial burden on agriculture. this study evaluated the efficacy of bacillus spp. as nematode biocontrol agents and identified bacillus genes associated with nematicidal activity. culture by products of bacillus subtilis strains okb105 and 69 and bacillus amyloliquefaciens strains fzb42 and b3 were used to treat aphelenchoides besseyi, ditylenchus destructor, bursaphelenchus xylophilus and meloidogyne java ...201121671997
expression of the lantibiotic mersacidin in bacillus amyloliquefaciens fzb42.lantibiotics are small peptide antibiotics that contain the characteristic thioether amino acids lanthionine and methyllanthionine. as ribosomally synthesized peptides, lantibiotics possess biosynthetic gene clusters which contain the structural gene (lana) as well as the other genes which are involved in lantibiotic modification (lanm, lanb, lanc, lanp), regulation (lanr, lank), export (lant(p)) and immunity (lanefg). the lantibiotic mersacidin is produced by bacillus sp. hil y-85,54728, which ...201121811596
bacillus amyloliquefaciens: a mosquitocidal bacterium from mangrove forests of andaman & nicobar islands, india.samples collected from the mangrove forests of andaman & nicobar islands yielded a mosquitocidal bacterium, whose extracellular metabolite(s) exhibited mosquito larvicidal and pupicidal activity. the bacterium was isolated using standard microbiological methods and identified using classical biochemical tests and rpob gene sequences. the mosquitocidal bacterium was identified as bacillus amyloliquefaciens. mosquitocidal metabolite(s) was separated from the culture supernatant of the bacterium an ...201121810402
study of phenol biodegradation using bacillus amyloliquefaciens strain wjdb-1 immobilized in alginate-chitosan-alginate (aca) microcapsules by electrochemical method.an aerobic microorganism with an ability to utilize phenol as sole carbon and energy source was isolated from phenol-contaminated wastewater samples. the isolate was identified as bacillus amyloliquefaciens strain wjdb-1 based on morphological, physiological, and biochemical characteristics, and 16s rdna sequence analysis. strain wjdb-1 immobilized in alginate-chitosan-alginate (aca) microcapsules could degrade 200 mg/l phenol completely within 36 h. the concentration of phenol was determined us ...201121809019
identification of a gene cluster that initiates azasugar biosynthesis in bacillus amyloliquefaciens. 201121786380
enhancement of the catalytic activity of a 27 kda subtilisin-like enzyme from bacillus amyloliquefaciens ch51 by in vitro mutagenesis.apre51 from bacillus amyloliquefaciens ch51 is a 27 kda subtilisin-like protease with fibrinolytic activity. to enhance the catalytic activity of apre51, two residues, gly-169 and ser-101, which, according to the three-dimensional structural model of subtilisin, are located in the p1 substrate-binding site and s3 subsite, respectively, were mutated by site-directed mutagenesis. results of the mutational analysis showed that substitution of alanine for gly-169 increased the fibrinolytic activity ...201121780825
production of 2,3-butanediol from glucose by gras microorganism bacillus amyloliquefaciens.in the current study, a gras (generally recognized as safe) strain of bacillus amyloliquefaciens producing 2,3-butanediol (2,3-bd) designated as b10-127 was isolated in our lab. the strain b10-127 produced 2,3-bd effectively under the condition of 20% glucose (quality concentration), showed a high-glucose tolerance. the effects of initial glucose concentration, temperature, ph and agitation on 2,3-bd production were investigated in this work and the proper parameters were identified. accordingly ...201121780143
[controlling effect of antagonist bioorganic fertilizer on tomato root-knot nematode].indoor in vitro culture experiment and greenhouse pot experiment were conducted to evaluate the capabilities of three bacterial strains xz-173 (bacillus amyloliquefaciens), sl-25 (b. gibsonii), and ks-62 (paenibacillus polymyxa) that can hydrolyze collagen protein in controlling tomato root-knot nematode. in the in vitro culture experiment, suspensions of xz-173, sl-25, and ks-62 induced a mortality rate of 75.9%, 66.7%, and 50.0% to the second-stage junior nematode within 24 h, and decreased th ...201121774329
bacillus amyloliquefaciens g1: a potential antagonistic bacterium against eel-pathogenic aeromonas hydrophila.recent studies have revealed that the use of probiotics is an alternative to control marine aeromonas. however, few probiotics are available against aeromonas hydrophila infections in eels. in the present study, a potential antagonistic strain g1 against the eel-pathogenic a. hydrophila was isolated from sediment underlying brackish water. its extracellular products with antibacterial activities were shown to be stable under wide range of ph, temperature, and proteinase k. it was initially ident ...201121754944
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