Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| identification of the gene encoding a 38-kilodalton immunogenic and protective antigen of streptococcus suis. | in our continued effort to search for a streptococcus suis protein(s) that can serve as a vaccine candidate or a diagnostic reagent, we constructed and screened a gene library with a polyclonal antibody raised against the whole-cell protein of s. suis type 2. a clone that reacted with the antibody was identified and characterized. analysis revealed that the gene encoding the protein is localized within a 2.0-kbp ecori dna fragment. the nucleotide sequence contained an open reading frame that enc ... | 2005 | 15817754 |
| generation of transposon insertion mutant libraries for gram-positive bacteria by electroporation of phage mu dna transposition complexes. | transposon mutagenesis is a powerful technique for generating collections of insertion mutants for genetic studies. this paper describes how phage mu dna transposition complexes, transpososomes, can be exploited for gene delivery to efficiently introduce selectable markers to genomes of gram-positive bacteria. mu transpososomes were assembled in vitro with custom-designed mini-mu transposons, concentrated, and electroporated into cells of three gram-positive bacterial species: staphylococcus aur ... | 2005 | 15817788 |
| the concentration of apolipoprotein a-i decreases during experimentally induced acute-phase processes in pigs. | in this work, apolipoprotein a-i (apoa-i) was purified from pig sera. the responses of this protein after sterile inflammation and in animals infected with actinobacillus pleuropneumoniae or streptococcus suis were investigated. decreases in the concentrations of apoa-i, two to five times lower than the initial values, were observed at 2 to 4 days. it is concluded that apoa-i is a negative acute-phase protein in pigs. | 2005 | 15845530 |
| [symposium "emerging zoonoses." dutch association for veterinary epidemiology and economy (veec)]. | 2005 | 15871390 | |
| serotyping and evaluation of the virulence in mice of streptococcus suis strains isolated from diseased pigs. | a total of 110 strains of streptococcus suis, isolated from diseased pigs in brazil were serotyped and analyzed for virulence. serotyping of the strains resulted in the following classification: 42 strains of serotype 2 (38.2%), 10 strains of serotype 14 (9.1%), seven strains of serotype 9 (6.4%), three strains each of serotype 7 and 11 (2.7%), two strains each of serotype 1 and 8 (1.8%) and one strain each of serotypes 1/2, 3, 5, 6 and 10 (0.9%). cross reactions among serotypes 1, 14 and 7 were ... | 2005 | 15880226 |
| first case report of streptococcus suis septicaemia and meningitis from greece. | 2005 | 15885057 | |
| dps/dpr ferritin-like protein: insights into the mechanism of iron incorporation and evidence for a central role in cellular iron homeostasis in streptococcus suis. | the dps family members constitute a distinct group of multimeric and ferritin-like iron binding proteins (up to 500 iron atoms/12-mer) that are widespread in eubacteria and archaea and implicated in oxidative stress resistance and virulence. despite the wealth of structural knowledge, the mechanism of iron incorporation has remained elusive. here, we provide evidence on dpr of the swine and human pathogen streptococcus suis that: (i) iron incorporation proceeds by fe(ii) binding, fe(ii) oxidatio ... | 2005 | 16091046 |
| outbreak associated with streptococcus suis in pigs, china. | 2005 | 16116899 | |
| infectious diseases. who probes deadliness of china's pig-borne disease. | 2005 | 16123268 | |
| human infective endocarditis caused by streptococcus suis serotype 2. | human infective endocarditis due to streptococcus suis occurs infrequently. the case of a 43-year-old male who sustained life-threatening infective endocarditis is reported. since growth of s. suis on commonly used sheep blood agar plates resembles viridans streptococci, diagnosis of s. suis constitutes a diagnostic pitfall. identification was accomplished by using molecular methods. | 2005 | 16145171 |
| acquisition of plasmin activity and induction of arachidonic acid release by streptococcus suis in contact with human brain microvascular endothelial cells. | infections caused by streptococcus suis, a major swine pathogen, include meningitis, arthritis, pneumonia and septicaemia. in this study, we investigated interactions that may occur between human brain microvascular endothelial cells (hbmec), the main constituent of the blood-brain barrier, and s. suis. we show that s. suis acquires plasmin activity in a time-dependent manner when in contact with cultured hbmec. cell-associated plasmin activity reached a plateau following a 48h co-incubation per ... | 2005 | 16182470 |
| dilemma of virulence of streptococcus suis: canadian isolate 89-1591 characterized as a virulent strain using a standardized experimental model in pigs. | virulence of streptococccus suis capsular type 2 strain 89-1591 has been controversial in literature. a standardized experimental model with specific-pathogen free piglets was used for a new evaluation of this strain. twenty-nine piglets were allotted in 4 separated groups. group 1 consisted of negative control animals which received broth medium. groups 2, 3, and 4 were intravenously challenged with 2 ml of s. suis, strains 1330, 89-1591, and 166', respectively. the strain 1330 is a recognized ... | 2005 | 16187555 |
| comparison and transferability of the erm (b) genes between human and farm animal streptococci. | to obtain better insights into the possible exchange of resistance genes between human and animal streptococci, the sequences of the erm (b) genes of streptococcal isolates from humans, pigs, pork carcasses, chickens, and calves were compared. identical erm (b) gene sequences were present in strains from humans, pigs, pork carcasses, and calves. during in vitro mating experiments, the erm (b) gene was exchanged between porcine streptococcus suis and human s. pneumoniae, s. pyogenes, and s. orali ... | 2005 | 16201935 |
| streptococcus suis infection. | a recent outbreak of streptococcus suis infection associated with the slaughter, preparation or consumption of pigs in sichuan, china has led to concerns that similar outbreaks could occur in other asian countries. although the pig farming industry is flourishing in taiwan, reports of s. suis infection remain rare. we report 2 cases of s. suis meningitis successfully treated with ceftriaxone and penicillin. previous reports of s. suis infection from the english literature are reviewed and the cl ... | 2005 | 16211137 |
| streptococcus suis, streptococcus mitis, what is the problem? | 2005 | 16219949 | |
| detection of extracellular factor-positive streptococcus suis serotype 2 strains in tonsillar swabs of live sows by pcr. | in this study, the sensitivity (se) and specificity (sp) of a pcr for the detection of ef-positive streptococcus suis serotype 2 strains in tonsillar swabs of live sows were assessed. we sampled 471 sows originating from four farrow-to-finish farms by tonsillar swabbing and collected their tonsils after slaughter. on these specimens, a pcr, a bacterial examination (be) or both were performed for the detection of ef-positive s. suis serotype 2 strains. swab-pcr, tonsil-pcr and tonsil-be were rega ... | 2005 | 16029935 |
| [acute meningitis caused by streptococcus suis: review of a case in a splenectomized patient]. | 2005 | 16411313 | |
| [determination of fibronectin-binding region of fbps of streptococcus suis type 2]. | fusion expression plasmid pfbps, pfbps (7 - 82), pfbps (7 - 165) and pfbps3 (87 - 320) of streptococcus suis type strain ha9801 were generated by cloning different fragments of fbps amplified from pmd-t-fbps by pcr into plasmid pet-32a(+). it has been confirmed that the recombinant proteins, fbps, fbps(87 -320), and fbps(7 - 167), which are expressed by recombinant plasmid pfbps, pfbps(87 - 320) and pfbps(7 - 165), respectively, bound human fibronectin by ligand affinity western blot assay. the ... | 2005 | 16342770 |
| the negative acute phase response of serum transthyretin following streptococcus suis infection in the pig. | transthyretin (ttr) is a serum protein which is a negative acute phase reactant in humans and levels of ttr are routinely measured as an indicator of health status. such tests have yet to be established for the pig. in order to measure serum ttr in the pig during an acute phase response an assay was developed using anti-human ttr antibodies which cross reacted with porcine ttr. the assay had a detection limit of 32 microg/ml while the mean concentration of transthyretin measured in healthy pig s ... | 2005 | 15955288 |
| prevalence of the suilysin gene in streptococcus suis strains isolated from diseased and healthy carrier pigs. | knowledge of virulence factors of streptococcus suis is limited. several virulence factor candidates have been proposed, among them suilysin, which is responsible for a toxic effect on epithelial cells. the aim of this study was to detect the suilysin gene sequence in streptococcus suis strains of various origin. in total 63 streptococcus suis isolates were investigated. forty four of them originated from tissues of streptococcosis affected animals. the remaining 19 strains were isolated from to ... | 2005 | 15989134 |
| pulmonary infections in swine induce altered porcine surfactant protein d expression and localization to dendritic cells in bronchial-associated lymphoid tissue. | surfactant protein d (sp-d) is a pattern-recognition molecule of the innate immune system that recognizes various microbial surface-specific carbohydrate and lipid patterns. in vitro data has suggested that this binding may lead to increased microbial association with macrophages and dendritic cells. the aim of the present in vivo study was to study the expression of porcine sp-d (psp-d) in the lung during different pulmonary bacterial infections, and the effect of the routes of infection on thi ... | 2005 | 16011521 |
| adherence of streptococcus suis to porcine endothelial cells. | streptococcus suis can cause invasive diseases in pigs and humans, such as meningitis or arthritis. adherence to and invasion of endothelial cells might represent important steps in survival and spread of s. suis within the host. we tested in vitro adherence and invasion of s. suis strains using a porcine brain microvascular and aortal endothelial cell line. four s. suis strains were tested with and without prior treatment with porcine serum containing anti-s. suis antibodies. strains included a ... | 2005 | 16283918 |
| [human-streptococcus suis infectious disease]. | 2005 | 16321218 | |
| serological characterization of haemophilus parasuis isolates from china. | from september 2002 to december 2004, a total of 281 strains of haemophilus parasuis were isolated from 17 provinces of china. all these isolates were serotyped by both the gel diffusion (gd) and the indirect haemagglutination (iha) tests. by combining the gd and iha results, serovar 4 (24.2%) and serovar 5 (19.2%) were the most prevalent serovars, followed by serovars 13 (12.5%), 14 (7.1%) and 12 (6.8%), while 12.1% of the isolates could not be assigned to a serovar (nontypable). a comparison o ... | 2005 | 16271834 |
| [highly virulent streptococcus suis infection and problems involved in the disease prevention and control in china]. | 2005 | 16471204 | |
| [anlysis on features of dead cases with human streptococcus suis infections]. | to describe the clinical and epidemiological features of dead cases with human streptococcus suis infections, and to find the target population for preventing death and the related indicators. | 2005 | 16471205 |
| [matched case-control study for risk factors of human streptococcus suis infection in sichuan province, china]. | to study the potential risk factors of human infecting with streptococcus suis. | 2005 | 16471206 |
| [detection of virulence-associated factors of streptococcus suis by multiplex pcr assay]. | to rapidly and sensitively detect the four virulence-associated factors of streptococcus suis, a multiplex pcr was developed. | 2005 | 16471207 |
| [human infection due to streptococcus suis]. | 2005 | 16471208 | |
| [streptococcus suis infection--clinical manifestations]. | streptococcus suis is a bacterium causing a disease in pigs and rarely in humans. this zoonosis is mostly found as a sporadic disease in individuals that were in contact with the affected or infected pigs: farmers, veterinarians and workers engaged in fresh pork processing. it is assumed that the bacterium enters the body through a cut abrasion in the skin. initially, the condition resembles a flu, followed by signs of bacteriemia and sepsis. the most frequent clinical manifestation of streptoco ... | 2005 | 16526227 |
| streptococcus suis meningitis with ventriculoperitoneal shunt infection and spondylodiscitis. | streptococcus suis is a zoonotic pathogen which causes meningitis, bacteremia, and endocarditis in pigs. human infection is rare and often presents as meningitis with the sequela of permanent deafness and endocarditis. previous cases were reported from pig-rearing countries such as holland and hong kong. we report a 55-year-old bedridden man with s. suis meningitis complicated with ventriculoperitoneal shunt infection and lumbar spine spondylodiscitis. he presented with fever, delirium, neck sti ... | 2005 | 16607454 |
| molecular characterization of streptococcus suis strains by 16s-23s intergenic spacer polymerase chain reaction and restriction fragment length polymorphism analysis. | we developed a new molecular method of typing streptococcus suis based on polymerase chain reaction (pcr) amplification of a large fragment of rrna genes, including a part of the 16s and 23s genes and the 16s-23s intergenic spacer region (isr), followed by restriction fragment length polymorphism (rflp) analysis with rsai or mboii endonuclease. the 16s-23s isrs of 5 s. suis isolates were sequenced and compared. size and sequence polymorphisms were observed between the s735 reference strain and t ... | 2006 | 16639941 |
| streptococcus suis infection: a series of 41 cases from chiang mai university hospital. | the objectives of this study were (1) to assess the clinical manifestations, treatment, and outcome of streptococcus suis infection in adult patients in northern thailand, (2) to evaluate the anti-microbial sensitivity pattern and (3) to determine the predicting factors of high mortality rate. | 2006 | 16690131 |
| proinflammatory cytokine and chemokine modulation by streptococcus suis in a whole-blood culture system. | streptococcus suis is an important swine and human pathogen. inflammation, a hallmark of s. suis infection, is thought to be responsible for most clinical signs of meningitis, septicaemia and sudden death. in this work, using a porcine whole blood model, s. suis serotype 2 was shown to trigger the release of several pro-inflammatory cytokines as evaluated by reverse transcriptase-pcr and enzyme-linked immunosorbent assay. although individual variations were observed among different s. suis strai ... | 2006 | 16706792 |
| human streptococcus suis outbreak, sichuan, china. | from mid-july to the end of august 2005, a total of 215 cases of human streptococcus suis infections, 66 of which were laboratory confirmed, were reported in sichuan, china. all infections occurred in backyard farmers who were directly exposed to infection during the slaughtering process of pigs that had died of unknown causes or been killed for food because they were ill. sixty-one (28%) of the farmers had streptococcal toxic shock syndrome; 38 (62%) of them died. the other illnesses reported w ... | 2006 | 16707046 |
| phagocytosis and killing of streptococcus suis by porcine neutrophils. | streptococcus suis serotype 2 is an important swine pathogen responsible for diverse infections, mainly meningitis. virulence factors and the pathogenesis of infection are not well understood. neutrophils may play an important role in the pathogenesis of infection given that infiltration by neutrophils and mononuclear cells are frequently observed in lesions caused by s. suis. the objective of this work was to study the interactions between s. suis serotype 2 and porcine neutrophils. results sho ... | 2006 | 16714092 |
| [meningitis due to streptococcus suis: a case report]. | 2006 | 16762264 | |
| [endocarditis and spondylodiscitis caused by streptococcus suis]. | 2006 | 16762266 | |
| the porcine acute phase protein response to acute clinical and subclinical experimental infection with streptococcus suis. | the pig acute phase protein (app) response to experimental streptococcus suis (s. suis) infection was mapped by the measurement of the positive apps c-reactive protein (crp), serum amyloid a (saa), haptoglobin (hp) and major acute phase protein (pig-map) and the negative apps albumin and apolipoprotein (apo) a-i. the aim was to elucidate the differences in the acute phase behaviour of the individual apps during a typical bacterial septicaemic infection. pigs were inoculated subcutaneously with l ... | 2006 | 16774789 |
| cell death, caspase activation, and hmgb1 release of porcine choroid plexus epithelial cells during streptococcus suis infection in vitro. | the choroid plexus epithelium constitutes the structural basis of the blood-cerebrospinal fluid barrier. we previously demonstrated that streptococcus suis (s. suis), a relevant cause of bacterial meningitis in pigs and humans, affects porcine choroid plexus epithelial cell (pcpec) barrier function and integrity. we now characterized pcpec cell death and investigated whether apoptosis or necrosis is responsible for the cytotoxicity after infection with different s. suis isolates. we found s. sui ... | 2006 | 16781680 |
| surface plasmon resonance biosensors for detection of pathogenic microorganisms: strategies to secure food and environmental safety. | this review describes the exploitation of exclusively optical surface plasmon resonance (spr) biosensors for the direct and indirect detection of pathogenic microorganisms in food chains and the environment. direct detection is, in most cases, facilitated by the use of defined monoclonal or polyclonal antibodies raised against (a part of) the target pathogenic microorganisms. the antibodies were immobilized to a solid phase of the sensor to capture the microbe from the sample. alternatively, ant ... | 2006 | 16792081 |
| [an outbreak of human streptococcus suis serotype 2 infections presenting with toxic shock syndrome in sichuan, china]. | in mid-july 2005, five patients presented with septic shock to a hospital in ziyang city in sichuan, china, to identify the etiology of the unknown reason disease, an epidemiological, clinical, and laboratory study were conducted. | 2006 | 16792880 |
| human streptococcus suis meningitis in the united states. | 2006 | 16554543 | |
| streptococcal toxic shock syndrome caused by streptococcus suis serotype 2. | streptococcus suis serotype 2 (s. suis 2, ss2) is a major zoonotic pathogen that causes only sporadic cases of meningitis and sepsis in humans. most if not all cases of streptococcal toxic shock syndrome (stss) that have been well-documented to date were associated with the non-ss2 group a streptococcus (gas). however, a recent large-scale outbreak of ss2 in sichuan province, china, appeared to be caused by more invasive deep-tissue infection with stss, characterized by acute high fever, vascula ... | 2006 | 16584289 |
| invasive disease and toxic shock due to zoonotic streptococcus suis: an emerging infection in the east? | 2006 | 16594733 | |
| [cloning and prokaryotic expression of truncated muramidase-released protein gene of human streptococcus suis type 2]. | to clone and express the truncated habb mrp gene of human streptococcus suis type 2 (s.suis 2) and detect its activity. | 2006 | 16507253 |
| [zoonotic importance of selected species of gram-positive bacteria.] | zoonoses continue to play a signicant role in both human and veterinary medicine. their eradication is difficult and some authors are convinced of their growing importance. among the pathogens of infectious diseases transmissible from animals to man are several gram-positive bacteria. the authors focus on three agents that present a risk in three different areas-alimentary infections (listeria monocytogenes), professional infections (streptococcus suis) and infections of breeders of so-called pe ... | 2006 | 16508905 |
| rational proteomics v: structure-based mutagenesis has revealed key residues responsible for substrate recognition and catalysis by the dehydrogenase and isomerase activities in human 3beta-hydroxysteroid dehydrogenase/isomerase type 1. | mammalian 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-hsd) is a member of the short chain dehydrogenase/reductase. it is a key steroidogenic enzyme that catalyzes the first step of the multienzyme pathway conversion of circulating dehydroepiandrosterone and pregnenolone to active steroid hormones. a three dimensional model of a ternary complex of human 3beta-hsd type 1 (3beta-hsd_1) with an nad cofactor and androstenedione product has been developed based upon x-ray structures of the ter ... | 2006 | 16889958 |
| meningitis and septicemia in a 7-week-old piglet caused by dual streptococcal infection. | a 7-week-old piglet was presented with a 2-hour history of neurological signs progressing to death. necropsy, bacteriologic culture, and immunohistochemical staining indicated an unusual case of streptococcus suis-like bacterial meningitis and s. suis type 3 septicemia. management and control of this important bacterial disease are discussed. | 2006 | 16933560 |
| streptococcus suis sequence type 7 outbreak, sichuan, china. | an outbreak of streptococcus suis serotype 2 emerged in the summer of 2005 in sichuan province, and sporadic infections occurred in 4 additional provinces of china. in total, 99 s. suis strains were isolated and analyzed in this study: 88 isolates from human patients and 11 from diseased pigs. we defined 98 of 99 isolates as pulse type i by using pulsed-field gel electrophoresis analysis of smai-digested chromosomal dna. furthermore, multilocus sequence typing classified 97 of 98 members of the ... | 2006 | 16965698 |
| reporter metabolite analysis of transcriptional profiles of a staphylococcus aureus strain with normal phenotype and its isogenic hemb mutant displaying the small-colony-variant phenotype. | in this study, full-genome dna microarrays based on the sequence of staphylococcus aureus n315 were used to compare the transcriptome of a clinical s. aureus strain with a normal phenotype to that of its isogenic mutant with a stable small-colony-variant (scv) phenotype (hemb::ermb). in addition to standard statistical analyses, systems biology advances were applied to identify reporter metabolites and to achieve a more detailed survey of genome-wide expression differences between the hemb mutan ... | 2006 | 16980462 |
| iron incorporation in streptococcus suis dps-like peroxide resistance protein dpr requires mobility in the ferroxidase center and leads to the formation of a ferrihydrite-like core. | the dps-like peroxide resistance protein (dpr) is a dodecameric protein that protects the human and swine pathogen streptococcus suis from hydrogen peroxide by removing free fe2+ from the cytosol. subsequent oxidation of iron by dpr results in the deposition of fe3+ inside the protein's central cavity. structural changes that occur in the ferroxidase center were studied by x-ray crystallography after soaking dpr crystals with fe2+ in the presence of sodium dithionite. twelve iron-binding sites w ... | 2006 | 16997323 |
| streptococcus suis endocarditis and colon carcinoma: a case report. | streptococcus bovis has classically been associated with colorectal carcinomas in literature. curiously enough, no other streptococcus species have hitherto been associated with such a malignancy or any other tumors. this report presents a case of early-stage colorectal carcinoma diagnosed in a patient with infection with the related streptococcus species s suis. a discussion of possible pathogenetic implications follows. | 2006 | 17026794 |
| [isolation and identification of streptococcus suis serotype 2 from sick-pig samples of sichuan province]. | streptococcus suis serotype 2 (ss2) is a major pathogen frequently associated with infections in pigs. there are presently 35 serotypes of s.suis (serotype 1 to 34 and serotype 1/2) recognized on the basis of capsular antigens. few people were reported to infect with ss2 in the past years. however, an accidental case happened in sichuan province of china in 2005. some people got ill and died, and all of them were closely contacted with sick pigs. based on clinical features and epidemiologic data ... | 2006 | 17037069 |
| identification of a novel virulence determinant with serum opacification activity in streptococcus suis. | streptococcus suis serotype 2 is a porcine and human pathogen with adhesive and invasive properties. in other streptococci, large surface-associated proteins (>100 kda) of the mscramm family (microbial surface components recognizing adhesive matrix molecules) are key players in interactions with host tissue. in this study, we identified a novel opacity factor of s. suis (ofs) with structural homology to members of the mscramm family. the n-terminal region of ofs is homologous to the respective r ... | 2006 | 17057090 |
| characteristics of streptococcus suis isolated from patients in japan. | seven cases of streptococcus suis infection in japan during 1994 and 2006 were summarized. all cases had porcine exposure and five of them had hand skin injury during the exposure. five cases presented symptoms of meningitis, three presented symptoms of sepsis, and one resulted in sudden death. all of the isolated s. suis belonged to lancefield's group d and to serotype 2. they were susceptible to penicillin g, ampicillin, cefotaxime, and ciprofloxacin. however, six of them were resistant to bot ... | 2006 | 17186962 |
| [changes of bacterial flora from hindguts of piglets after oral administration of lactobacillus amylovorus s1 as a probiotic strain]. | changes of bacterial flora from hindguts of piglets from 7 to 35 days of age (two weeks after weaning) were studied after oral administration of l. amylovorus s1, using molecular techniques based on 16s rdna gene. six litters of neonatal piglets were divided randomly into control group and treatment group. at 7, 9, 11 days of age, piglets in treatment group received 1, 2 and 3ml preparation of s1 (5 x 10(9) cfu/ml) through oral administration, respectively. on d 7, 14, 21, 24 and 35, one piglet ... | 2006 | 17302162 |
| [cloning and characterization of the gene encoding impdh of streptococcus suis serotype 2]. | given the lack of effective vaccines to control streptococcus suis infection and the lack of a rapid and reliable molecular diagnostic assay to detect its infection, s. suis serotype 2 was sequenced partly in an effort to identify important virulence factors. two new open reading frames were found located between orf2 and mrp. one of new open reading frame (2738 - 3694) that encoded a polypeptide of 319 amino acid residues with a calculated molecular mass of 33.5kda was identified by western blo ... | 2006 | 17172018 |
| upregulation of prostaglandin e2 and matrix metalloproteinase 9 production by human macrophage-like cells: synergistic effect of capsular material and cell wall from streptococcus suis. | streptococcus suis, an early colonizer of the upper respiratory tract, is a major swine pathogen worldwide that can cause meningitis, arthritis, pneumonia, and septicemia. while numerous studies on potential virulence factors of s. suis have been carried out over the past decade, the exact mechanisms by which this bacterium invades the host and migrates through the blood brain barrier (bbb) remain unclear. in the study presented here, we show that whole cells of s. suis were able to upregulate t ... | 2006 | 16324819 |
| identification of a surface protein of streptococcus suis and evaluation of its immunogenic and protective capacity in pigs. | a streptococcus suis surface protein reacting with convalescent-phase sera from pigs clinically infected by s. suis type 2 was identified. the apparent 110-kda protein, designated sao, exhibits typical features of membrane-anchored surface proteins of gram-positive bacteria, such as a signal sequence and an lpvtg membrane anchor motif. in spite of high identity with the partially sequenced genomes of s. suis canadian strain 89/1591 and european strain p1/7, sao does not share significant homolog ... | 2006 | 16368985 |
| structure, regulation, and putative function of the arginine deiminase system of streptococcus suis. | streptococcus suis is an important cause of infectious diseases in young pigs. little is known about the virulence factors or protective antigens of s. suis. recently, we have identified two proteins of the arginine deiminase system (ads) of s. suis, which were temperature induced and expressed on the streptococcal surface (n. winterhoff, r. goethe, p. gruening, m. rohde, h. kalisz, h. e. smith, and p. valentin-weigand, j. bacteriol. 184:6768-6776, 2002). in the present study, we analyzed the co ... | 2006 | 16385025 |
| quantitative susceptibility of streptococcus suis strains isolated from diseased pigs in seven european countries to antimicrobial agents licensed in veterinary medicine. | the susceptibility of streptococcus suis strains (n=384) isolated from diseased pigs in seven european countries to 10 antimicrobial agents was determined. for that purpose a microbroth dilution method was used according to clsi recommendations. the following antimicrobial agents were tested: ceftiofur, cefquinome, enrofloxacin, florfenicol, gentamicin, penicillin, spectinomycin, tetracycline, tilmicosin and trimethoprim/sulphamethoxazole. using breakpoints established by clsi for veterinary pat ... | 2006 | 16387456 |
| virulence-associated gene profiling of streptococcus suis isolates by pcr. | definition of virulent streptococcus suis strains is controversial. one successful approach for identification of virulent european strains is differentiation of capsular serotypes (or the corresponding cps types) and subsequent detection of virulence-associated factors, namely the extracellular factor (ef, epf), the muramidase-released protein (mrp, mrp) and the hemolysin suilysin (sly, sly). in this work we present a novel multiplex pcr (mp-pcr) and an mrp variant pcr for identification and ch ... | 2006 | 16431041 |
| chronic meningitis in thailand. clinical characteristics, laboratory data and outcome in patients with specific reference to tuberculosis and cryptococcosis. | the charts of 114 consecutive patients with chronic meningitis admitted to a general hospital in bangkok, thailand, between 1993 and 1999 were retrospectively reviewed. the most common causative agents were cryptococcus neoformans (54%) and mycobacterium tuberculosis (37%). hiv and other underlying diseases had a major impact on the presentation of chronic cryptococcal meningitis patients. compared to hiv-negative cryptococcal meningitis patients (21%), hiv-positives (79%) had a significantly lo ... | 2006 | 16254452 |
| amplified fragment length polymorphism of streptococcus suis strains correlates with their profile of virulence-associated genes and clinical background. | amplified fragment length polymorphism (aflp) typing was applied to 116 streptococcus suis isolates with different clinical backgrounds (invasive/pneumonia/carrier/human) and with known profiles of virulence-associated genes (cps1, -2, -7 and -9, as well as mrp, epf and sly). a dendrogram was generated that allowed identification of two clusters (a and c) with different subclusters (a1, a2, c1 and c2) and two heterogeneous groups of strains (b and d). for comparison, three strains from each aflp ... | 2007 | 17172524 |
| a glimpse of streptococcal toxic shock syndrome from comparative genomics of s. suis 2 chinese isolates. | streptococcus suis serotype 2 (ss2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. very recently, ss2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (stss), which was originally associated with streptococcus pyogenes (gas) in streptococci. however, the molecular mechanisms underlying stss are poorly understood. | 2007 | 17375201 |
| interactions between streptococcus suis serotype 2 and cells of the myeloid lineage in the palatine tonsil of the pig. | streptococcus suis is a major pathogen in pigs and causes significant morbidity and mortality in herds world-wide. a major problem with s. suis is the presence of asymptomatic carrier animals which can spread the organism within and between herds. the palatine tonsil is one of the main sites where the organism can be recovered, both in infected and carrier animals. the use of multiple-colour immunohistology allowed identification of the cell types associated with bacteria in the tonsils of infec ... | 2007 | 17397935 |
| immunoproteomics of extracellular proteins of chinese virulent strains of streptococcus suis type 2. | streptococcus suis type 2 (ss2) is a porcine zoonotic pathogen with worldwide distribution, and lacking suitable vaccine and virulent maker were bottleneck to control this infection. an immunoproteomic assay was used to identify antigenic proteins from the total extracellular proteins of the virulent chinese ss2 strain zy05719. the convalescent serum of a specific pathogen free (spf) mini-pig recognized nine protein spots on pvdf membrane. antigenic proteins on a duplicate gel, as well as those ... | 2007 | 18022935 |
| [cloning, expression and characterization analysis of the arginine deiminase of streptococcus suis of china isolates]. | pcr analysis demonstrated the presence of the ad gene in all 29 s. suis strains tested, but none of the seven s. equi subsp. zooepidemicus strains. the fragment of ad gene of virulent isolate ss2-ha9801 was later cloned into pbad/myc-hisc vector via restriction endonuclease and then transformed into host strain top10. a recombinant protein of 47000da was highly expressed after induced by l-ararose and purified by ni-nitrilotriacetic acid affinity chromatography. western blotting demonstrated tha ... | 2007 | 18062263 |
| high-throughput sequence alignment using graphics processing units. | the recent availability of new, less expensive high-throughput dna sequencing technologies has yielded a dramatic increase in the volume of sequence data that must be analyzed. these data are being generated for several purposes, including genotyping, genome resequencing, metagenomics, and de novo genome assembly projects. sequence alignment programs such as mummer have proven essential for analysis of these data, but researchers will need ever faster, high-throughput alignment tools running on ... | 2007 | 18070356 |
| [study on expression of streptococcus suis serotype 2 sly gene, purification and activity of its product]. | to clone and express streptococcus suis serotype 2 (s. suis 2) sly gene for constructing an foundation on identification of s. suis 2 protective antigen. | 2007 | 18476581 |
| [identification and characterization of a novel antigenic protein of streptococcus suis type 2]. | an immunogenic protein, named hm3, of streptococcus suis type 2 ha9801 was identified by using immunoproteomic assay. some characters of this protein were analyzed by several online bioinformatical tools, including blast, singlp, hmmtop and psortb. the most homologous sequence of hm3 was extracellular solute-binding protein (gi/69246104) of enterococcus faecium. the predictions results showed that hm3 contained signal peptide and one transmembrane region, and non-cytoplasmic localization were al ... | 2007 | 18271262 |
| tlr2-dependent recognition of streptococcus suis is modulated by the presence of capsular polysaccharide which modifies macrophage responsiveness. | streptococcus suis capsular type 2 is an important swine pathogen and an agent of zoonosis. although meningitis is the most common form of disease, septicemia and septic shock are also frequently reported. despite reports that cd14 is involved in the recognition of encapsulated s. suis by host cells, the mechanisms underlying exacerbated release of pro-inflammatory cytokines, which may have a negative impact on disease outcome, are unclear. here, we demonstrated that stimulation of human monocyt ... | 2007 | 17307800 |
| streptococcus suis: an emerging zoonotic pathogen. | streptococcus suis is a major porcine pathogen worldwide, and can be transmitted to human beings by close contact with sick or carrier pigs. s suis causes meningitis, septicaemia, endocarditis, arthritis, and septic shock in both pigs and human beings, and mortality is high. human infection with s suis occurs mainly among certain risk groups that have frequent exposure to pigs or pork. outbreaks of human s suis infection are uncommon, although several outbreaks have occurred in china in recent y ... | 2007 | 17317601 |
| detection and molecular typing of streptococcus suis in tonsils from live pigs in france. | streptococcus suis is an important pathogen of swine, causing meningitis, arthritis, polyserositis, septicemia, and sudden death in weaning piglets as well as fattening pigs. recently, 3 molecular tests have been developed in our laboratory: a multiplex polymerase chain reaction (m-pcr) assay for the detection of s. suis species and serotypes 2 and 1/2, and 2 molecular typing methods, pulsed-field gel electrophoresis and an approach based on pcr amplification of a fragment of rrna genes, includi ... | 2007 | 17193877 |
| variant size- and glycoforms of the scavenger receptor cysteine-rich protein gp-340 with differential bacterial aggregation. | glycoprotein gp-340 aggregates bacteria in saliva as part of innate defence at mucosal surfaces. we have detected size- and glycoforms of gp-340 between human saliva samples (n = 7) and lung gp-340 from a proteinosis patient using antibodies and lectins in western blots and elisa measurements. western blots of saliva samples, and of gp-340 purified, from the seven donors using a gp-340 specific antibody distinguished four gp-340 size variants, designated i to iv (n = 2,2,2 and 1). while saliva g ... | 2007 | 17243023 |
| a novel signature-tagged mutagenesis system for streptococcus suis serotype 2. | streptococcus suis is an economically important, zoonotic pathogen causing death and disease in swine. the objectives of this study were to develop a signature-tagged mutagenesis (stm) system for s. suis serotype 2 and to identify genes required for in vivo virulence. identification of such candidate genes may lead to a better understanding of the pathogenesis of s. suis and may provide substrate for the discovery of new vaccines. a novel stm approach was designed to allow for a higher throughpu ... | 2007 | 17275218 |
| potential use of an unencapsulated and aromatic amino acid-auxotrophic streptococcus suis mutant as a live attenuated vaccine in swine. | streptococcus suis is responsible for severe economic losses to the swine industry. prevention of the diseases caused by this pathogen is hampered by the inability of available vaccines to generate a protective response in pigs. a non-virulent, aromatic amino acid-auxotrophic and unencapsulated mutant strain of s. suis was generated in this study and a preliminary evaluation of its protective capacities was conducted in swine. deletion of the cognate promoter of the aro operon in s. suis virulen ... | 2007 | 17296252 |
| prevalence of streptococcus suis genotypes in wild boars of northwestern germany. | invasive serotype 2 (cps2+) strains of streptococcus suis cause meningitis in pigs and humans. four case reports of s. suis meningitis in hunters suggest transmission of s. suis through the butchering of wild boars. therefore, the objective of this study was to investigate the prevalence of potentially human-pathogenic s. suis strains in wild boars. s. suis was isolated from 92% of all tested tonsils (n=200) from wild boars. a total of 244 s. suis isolates were genotyped using pcr assays for the ... | 2007 | 17085699 |
| first characterization of fluoroquinolone resistance in streptococcus suis. | we have identified and sequenced the genes encoding the quinolone-resistance determining region (qrdr) of parc and gyra in fluoroquinolone-susceptible and -resistant streptococcus suis clinical isolates. resistance is the consequence of single point mutations in the qrdrs of parc and gyra and is not due to clonal spread of resistant strains or horizontal gene transfer with other bacteria. | 2007 | 17116660 |
| rapid pulsed-field gel electrophoresis protocol for subtyping of streptococcus suis serotype 2. | a rapid pulsed-field gel electrophoresis (pfge) protocol for subtyping of streptococcus suis serotype 2 was developed and evaluated using 27 clinical isolates from 22 epidemiologically unrelated patients. results were matched against antibiogram, virulence genotyping and multi locus sequence typing (mlst). pfge appeared to be the most discriminatory with numerical index of discrimination (d) equal to 0.87. | 2007 | 17157941 |
| neurological infections: clinical advances and emerging threats. | 2007 | 17166795 | |
| streptococcus suis in hong kong. | streptococcus suis was isolated from 6.1% of raw pork meat from 3 of the 6 wet markets in 6 districts in hong kong. s. suis was particularly isolated in sites from the tongue, tonsil, bone, and tail, but not from lean meat/minced pork or internal organs. isolates were confirmed by polymerase chain reaction using s. suis-specific primers, did not belong to serotype 2 using serotype 2-specific antiserum, and were clustered closely with other known serotypes by phylogenetic analysis. ten strains fr ... | 2007 | 16860513 |
| meningitis due to streptococcus suis with no contact with pigs or porcine products. | 2007 | 17434590 | |
| characterization of the invasion of porcine endothelial cells by streptococcus suis serotype 2. | streptococcus suis serotype 2 is an important swine pathogen associated mainly with meningitis. in a previous study, we demonstrated the ability of s. suis serotype 2 to adhere to and invade immortalized porcine brain microvascular endothelial cells (pbmecs) forming the blood-brain barrier. the aim of the current work was to further characterize the mechanism(s) by which s. suis invades porcine endothelial cells. the ability of several s. suis strains to interact with pbmecs was not found to cor ... | 2007 | 17479770 |
| use of selective capture of transcribed sequences to identify genes preferentially expressed by streptococcus suis upon interaction with porcine brain microvascular endothelial cells. | by using the selective capture of transcribed sequences (scots) approach, we identified 28 genes preferentially expressed by the major swine pathogen and zoonotic agent streptococcus suis upon interaction with porcine brain microvascular endothelial cells. several of these genes may be considered new s. suis candidate virulence factors. results from this study demonstrate the suitability of scots for the elucidation of gene expression in streptococcal species and may contribute to a better under ... | 2007 | 17483264 |
| elimination of strains of streptococcus suis serotype 2 from the tonsils of carrier sows by combined medication and vaccination. | the effect of vaccination with a killed whole-cell vaccine of extracellular factor-positive streptococcus suis serotype 2 (s suis 2 ef(+)) combined with medication with amoxicillin on the presence of virulent s suis 2 ef(+) strains on the tonsils of sows and their offspring was evaluated. in two herds, 14 pregnant sows that carried these virulent strains, as detected by pcr in three consecutive tonsillar brush samples, were selected and randomly assigned to be treated or left untreated as contro ... | 2007 | 17483379 |
| [homologous analysis of streptococcus suis srt gene and prokaryotic expression of srta]. | to elucidate the distribution of srt gene in the genome of s.suis 05zyh33, prokaryotically express sortase a and analyze its antigenicity. | 2007 | 17488596 |
| [meningitis caused by streptococcus suis]. | a 42-year-old man was admitted to hospital because of headache and fever for two days and showing signs of meningism. as a butcher he had had frequent cuts on his hands and forearms. | 2007 | 17492568 |
| an outbreak of streptococcus suis in chengdu, china. | 2007 | 17504369 | |
| mobilomefinder: web-based tools for in silico and experimental discovery of bacterial genomic islands. | mobilomefinder (http://mml.sjtu.edu.cn/mobilomefinder) is an interactive online tool that facilitates bacterial genomic island or 'mobile genome' (mobilome) discovery; it integrates the arrayome and trnacc software packages. arrayome utilizes a microarray-derived comparative genomic hybridization input data set to generate 'inferred contigs' produced by merging adjacent genes classified as 'present'. collectively these 'fragments' represent a hypothetical 'microarray-visualized genome (mvg)'. ar ... | 2007 | 17537813 |
| [investigation of a possible chain of streptococcus suis infection in a pig breeding community using pcr-based genotyping]. | streptococcus (s.) suis plays an important role in pig breeding causing invasive diseases such as meningitis and polyarthritis. herd problems with streptococcus suis are common in many pig farms and are frequently characterised by disease outbreaks. in this context, it is often important to identify chains of infection, e.g. between a farrow-to-wean and a grower farm. in the following case report a possible chain of infection among the different farms of a farrow-to-finish system was investigate ... | 2007 | 17555039 |
| adhesion activity of glyceraldehyde-3-phosphate dehydrogenase in a chinese streptococcus suis type 2 strain. | a total of 36 streptococcal strains, including seven s. equi ssp.zooepidemicus, two s. suis type 1 (ss1), 24 ss2, two ss9, and one ss7, were tested for glyceraldehyde-3-phosphate dehydrogenase gene (gapdh). except from non-virulent ss2 strain t1 5, all strains harboured gapdh. the gapdh of chinese sichuan ss2 isolate zy05719 and jiangsu ss2 isolate ha9801 were sequenced and then compared with published sequences in the genbank. the comparison revealed a 99.9 % and 99.8 % similarity of zy05719 an ... | 2007 | 17555040 |
| immunization with recombinant sao protein confers protection against streptococcus suis infection. | sao is a streptococcus suis surface protein recently identified as a potential vaccine candidate. in this study, recombinant sao in combination with quil a provided cross-protection against s. suis serotype 2 disease in mouse and pig vaccination protocols. subcutaneous immunization of mice elicited strong immunoglobulin g (igg) antibody responses. all four igg subclasses were induced, with the igg2a titer being the highest, followed by those of igg1, igg2b, and igg3. challenge of the mice with s ... | 2007 | 17567767 |
| human meningitis caused by streptococcus suis: the first case report from north-eastern italy. | we describe the first case of streptococcus suis meningitis in the north-east of italy. we would like to stress that streptococcus suis should be seriously considered in the differential diagnosis of human meningitis especially in adults with a recent history of close contact with pigs or unprocessed pork meat. | 2007 | 17598998 |
| development and evaluation of an immunochromatographic strip for detection of streptococcus suis type 2 antibody. | in this study, an immunochromatographic strip (ics) was developed for the detection of antibody against streptococcus suis serotype 2 (ss2). colloidal gold particles labeled with staphylococcal protein a (spa), which can bind to the f(c) fragment of mammalian immunoglobulin, were used as the detector reagent. the capsular polysaccharide (cps) of ss2 and affinity-purified igg from a healthy naive pig were immobilized on test and control regions of a nitrocellulose membrane, respectively. the ics ... | 2007 | 17609343 |
| inhibition of p-fimbriated escherichia coli adhesion by multivalent galabiose derivatives studied by a live-bacteria application of surface plasmon resonance. | uropathogenic p-fimbriated escherichia coli adheres to host cells by specific adhesins recognizing galabiose (galalpha1-4gal)-containing structures on cell surfaces. in search of agents inhibiting this first step of infection, the inhibition potency of a set of synthetic mono- and multivalent galabiose compounds was evaluated. in order to mimic the flow conditions of natural infections, a live-bacteria application of surface plasmon resonance (spr) was established. | 2007 | 17623698 |
| [meningoencephalitis related-to streptococcus suis in a butcher: relevance of occupational questionnaire at emergency room]. | 2007 | 17629656 | |
| streptococcus suis serotype 2, an important swine and human pathogen, induces strong systemic and cerebral inflammatory responses in a mouse model of infection. | streptococcus suis, an important swine and human pathogen, causes septic shock and meningitis. the pathogenesis of both systemic and cns infections caused by s. suis is poorly understood. a hematogenous model of infection in cd1 mice was developed to study the systemic release of cytokines during the septic shock phase and the proinflammatory events in the cns associated with this pathogen. using a liquid array system, high levels of systemic tnf-alpha, il-6, il-12, ifn-gamma, ccl2, cxcl1, and c ... | 2007 | 17641051 |
| streptococcus suis infections in humans: the chinese experience and the situation in north america. | infections caused by streptococcus suis are considered a global problem in the swine industry. in this animal species, s. suis is associated with septicemia, meningitis, endocarditis, arthritis and, occasionally, other infections. moreover, it is an agent of zoonosis that afflicts people in close contact with infected pigs or pork-derived products. although sporadic cases of s. suis infection in humans have been reported, a large outbreak due to s. suis serotype 2 emerged in the summer of 2005 i ... | 2007 | 17692141 |