Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
residual bacterial contamination on reusable pulse oximetry sensors.an early study demonstrated that standard cleaning techniques did not adequately disinfect reusable pulse oximetry sensors that had been intentionally contaminated with high titers of pathogenic microorganisms. this current study evaluated patient-ready sensors being used in hospitals, by testing them for bacterial contamination.199310145923
clinical and microbiologic aspects of staphylococcus haemolyticus infections. 19938108209
a comparative study on growth in soft-agar, adherence to glass and haemolysis types of coagulase-negative staphylococci.growth properties of coagulase-negative staphylococci in the presence and in the absence of human and rabbit serum in soft-agar prepared in modified staphylococcus 110 broth were studied. the adherent growth was examined in modified staphylococcus 110 broth and 1% glucose-meat broth. of 100 strains examined 69% exhibited diffuse, 18% compact, 7% transient and 6% mixed growth. compact type colonies were mainly characteristic of staphylococcus haemolyticus strains. the presence of serum failed to ...19938191864
a study of the epidemiology of an endemic strain of staphylococcus haemolyticus (tor-35) in a neonatal intensive care unit.coagulase-negative staphylococci (cns) are among the most prevalent microorganisms that colonize and cause sepsis in neonatal intensive care units (nicu). we had previously identified a strain of cns, staphylococcus haemolyticus (tor-35), in the nicu at mount sinai hospital, that had been repeatedly isolated from blood cultures from neonates. we therefore carried out a prospective study to determine the frequency and time of colonization and the frequency of bacteremia in neonates over a 3.5 mon ...19938248752
internal bacterial flora of solid uterine cervical cancer.twenty-five patients with uterine cervical cancer (two cases stage o; four cases stage ia; five cases stage ib; one case stage iia; ten cases stage iib; two cases stage iiia; one case stage iva) served as the subjects. the bacterial flora inside the cervical cancers was investigated using the optimal technique. there was mixed abnormal aerobic and anaerobic bacterial flora in all subjects. the average number of bacterial species isolated from inside the cervical cancers was 6.3. the predominant ...19938270797
infection problems for the 1990's--do we have an answer?there is and has been continued change in organisms causing infection in the hospital. in the past few years, although gram-negative bacteria have remained a major cause of mortality, gram-positive bacteria and fungi have become increasingly important. this has caused organisms such as methicillin-resistant staphylococci, enterococci, xanthomonas maltophilia and multiply resistant pseudomonas aeruginosa to be common pathogens. can this difficult state of affairs be changed by better antimicrobia ...19938290904
[study on mec gene in methicillin-resistant staphylococci].methicillin-resistant staphylococcus aureus (mrsa), methicillin-susceptible staphylococcus aureus (mssa), methicillin-resistant staphylococcus epidermidis and methicillin-resistant staphylococcus haemolyticus strains which were isolated in 1992 were surveyed for distribution of meca, mecr1 (putative meca gene inducer gene) and meci gene (putative meca gene repressor gene) by the method of polymerase chain reaction (pcr). all the 30 strains (100%) of mrsa carried meca gene and mecr1 gene-1 (32-35 ...19938294771
cell wall deficient bacteria as a cause of idiopathic hematuria.idiopathic hematuria in the absence of bacteriuria is a medical challenge. routine cultures of catheterized bladder and endoscopically obtained ureteral urine specimens from a 22-year-old woman with a 6-week history of hematuria showed no growth after 24 to 48 hours of incubation. however, bacterial variants were grown on enriched media. colonies were typical cell wall deficient/defective bacteria. phase and electron microscopy of cystoscopic urine specimens obtained by retrograde ureteral cathe ...19938326586
[pathogenic properties of coagulase-negative staphylococci in experimental infections].the frequency of persistence of three staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus saprophyticus strains, respectively, was studied in balb/c mice at the 10th day of intraperitoneal (ip) challenge. 245 out of 416 mice survived after infections with four bacterial suspensions of different colony forming units (cfu) of each strain. staphylococci persisted in 61 mice (24,9%). the main sites of persistence were the kidneys, while cocci were rarely isolated from the sple ...19938446403
trends in gram-positive bloodstream organism resistance: a seven-year audit of five glycopeptides and other drugs at a large university hospital.gram-positive pathogens (n = 525) isolated from bloodstream infections were tested by a reference broth microdilution method to establish antibiotic susceptibility trends (1985 to 1991). cefazolin, ciprofloxacin, gentamicin, novobiocin, oxacillin, rifampin, teicoplanin, vancomycin, trimethoprim/sulfamethoxazole, daptomycin and two investigational glycopeptides (ly264826 and mdl62873) were investigated. strains were selected without bias (first two isolates each month/species; no patient duplicat ...19938459260
occurrence of macrolide-lincosamide-streptogramin resistances among staphylococcal clinical isolates at a university medical center. is false susceptibility to new macrolides and clindamycin a contemporary clinical and in vitro testing problem?a total of 2189 staphylococcal strains at the university of iowa hospitals and clinics (iowa city, ia) were initially screened to determine the incidence of constitutive (29.8%) and potential inducible macrolide-lincosamide-streptogramin (mls) resistance (11.3%). staphylococcus haemolyticus and s. epidermidis (62.5% and 55.3%) showed the highest incidence of constitutive resistance. staphylococcus hominis had the highest incidence of inducible resistance (40.6%), while s. aureus had the lowest r ...19938477574
[immunosuppressive effect of cyclophosphamide in experimental coagulase-negative staphylococcal infection].balb/c mice were given 200 mg/kg cyclophosphamide intraperitoneally. three days later, mice were infected intraperitoneally with staphylococcus epidermidis, staphylococcus haemolyticus, staphylococcus saprophyticus at 4 inocula ranging between 2 x and 20 x 10(8) cfu/ml suspended in dextran microcarrier solution. controls were treated only with bacteria. lethality rates and organ persistence of cocci were significantly higher, and more peritoneal abscesses and adhesions developed in the mice pret ...19938497387
comparative prophylactic efficacies of ciprofloxacin, ofloxacin, cefazolin, and vancomycin in experimental model of staphylococcal wound infection.recent shifts in the species and antibiotic resistance patterns of bacteria causing nosocomial infections present new challenges for providing effective prophylaxis in surgery. traditional regimens lack activity against methicillin-resistant staphylococci and many gram-negative species causing nosocomial infections. the new fluoroquinolones exhibit in vitro activity against many emerging surgical wound pathogens. to determine the potential of this class of antimicrobial agents for use in surgery ...19948092833
posttraumatic meningitis: bacteriology, hydrocephalus, and outcome.to investigate the conditions that have developed in the treatment of posttraumatic meningitis with the use of new antibiotics, the authors studied cases with this infection retrospectively for a period of 68 months. among 860 patients with moderate to severe head injuries, 12 (1.39%) sustained this complication. of these, nine patients (75%) had a demonstrable basilar skull fracture and seven (58.3%) presented obvious rhinorrhea. of these seven, four (57.1%) were treated conservatively and thre ...19947800133
in vitro activity of fk-037, a novel parenteral cephalosporin, against bacterial isolates from neutropenic cancer patients.the in vitro activity of fk-037, a novel parenteral cephalosporin, was compared to that of ceftazidime, aztreonam and piperacillin (agents often used in empiric regimens in cancer patients) against recent bacterial isolates from patients with cancer. fk-037 was either equal to or 2 to 16-fold more active than the comparative agents against members of the enterobacteriaceae. it was also active against acinetobacter spp., aeromonas spp., pseudomonas aeruginosa, and other pseudomonas spp. xanthomon ...19947813504
nosocomial infection by staphylococcus haemolyticus and typing methods for epidemiological study.a patient with chronic myelogenous leukemia became colonized with a staphylococcus haemolyticus strain and experienced a septic episode caused by this strain during a cytostatic course. the strain was multiply resistant to antibiotics; the mic and mbc of vancomycin were 2 and 4 mg/liter, and the mic and mbc of teicoplanin were 4 and 16 mg/liter, respectively. we performed a surveillance study on the carriage of s. haemolyticus in medical and nursing staff of the hospital ward where the patient w ...19947529252
rapd typing of clinical isolates of staphylococcus haemolyticus.the randomly amplified polymorphic dna (rapd) assay was used to generate dna fingerprints from clinical isolates of staphylococcus haemolyticus isolated from patients treated with continuous ambulatory peritoneal dialysis and previously subjected to a combination of typing methods. the rapd profiles generated with one of six randomly designed 10-mer primers allowed visual discrimination of strains. good correlation with the original typing scheme was achieved but rapd typing allowed discriminati ...19947764597
relatedness of coagulase-negative staphylococci causing bacteremia in low-birthweight infants.to investigate coagulase-negative staphylococcus (cons) causing bacteremia in a neonatal intensive care unit (nicu).19947844337
distribution of coagulase-negative staphylococci, including the newly described species staphylococcus schleiferi, in nosocomial and community acquired urinary tract infections.four hundred and four coagulase-negative staphylococci were isolated from 4905 urine specimens obtained from 4192 inpatients and outpatients. the distribution of the strains was as follows: 193 staphylococcus epidermidis (47.8%), 171 staphylococcus saprophyticus (42.3%), 29 staphylococcus haemolyticus (7.2%), 5 staphylococcus warneri (1.2%), 3 staphylococcus schleiferi (0.7%), 2 staphylococcus hominis (0.5%) and 1 staphylococcus simulans (0.2%). all three staphylococcus schleiferi strains were i ...19947889974
dissemination among staphylococci of dna sequences associated with methicillin resistance.dna probes consisting of puc19 containing cloned staphylococcus aureus chromosomal fragments were constructed from two methicillin-resistant s. aureus strains with different dna sequences 5' to meca, the gene that mediates methicillin resistance. the probe from one strain, bms1, contained a portion of the regulatory sequences (the terminal 641 bp of mecr1 and all of meci) associated with the induction and repression of meca transcription (pgo195). the second probe, from strain col (pgo198), cont ...19947911288
activation of the human immunodeficiency virus long terminal repeat in thp-1 cells by a staphylococcal extracellular product.staphylococcal strains can release a factor that strongly activates the human immunodeficiency virus type 1 (hiv-1) long terminal repeat (ltr) in thp-1 cells transfected with the hiv-1 ltr-driven luciferase reporter gene (thp-1 ltrluc). the factor is present in the overnight culture fluid and is readily released from the organisms into aqueous medium by vigorous mixing. staphylococcal extracellular material is a complex mixture of polysaccharide and protein containing peptidoglycan and teichoic ...19947938001
pathogenicity and virulence of coagulase negative staphylococci in relation to adherence, hydrophobicity, and toxin production in vitro.to study the pathogenicity and virulence characteristics of staphylococcus epidermidis, staphylococcus haemolyticus, and staphylococcus sapro-phyticus.19947962630
rapid identification of micro-organisms from urinary tract infections by beta-glucuronidase, phenylalanine deaminase, cytochrome oxidase and indole tests on isolation media.two commercially available media recommended for the isolation and rapid identification of escherichia coli from urinary tract infections were supplemented with l-phenylalanine and l-tryptophan. the non-selective medium proved suitable for the direct detection of lactose fermentation, beta-glucuronidase and phenylalanine deaminase activities, indole production and the oxidase test. it was highly efficient in making a presumptive identification at species level of the most common gram-negative ur ...19947966214
[numerical taxonomy of staphylococci isolated from water and beach sand from valparaíso and viña del mar, chile].a total of 85 strains of gram positive cocci isolated from beach water and sand located in valparaiso bay, and viña del mar, chile, were examined for 59 morphological, physiological and biochemical characteristics. the taxonomic data were analysed by numerical taxonomy using ssm coefficient and the unweighted pair group method of association (upgma). at 80% similarity level, four phenons were obtained. of the 85 strains 31% were classified as staphylococcus epidermidis, 9% were classified as sta ...19947973182
identification of coagulase-negative staphylococci and typing of staphylococcus epidermidis by a 4 h micromethod.a new 4 h micromethod (minibact-s) for identification of coagulase-negative staphylococci (cns) important in human medicine, staphylococcus epidermidis, staphylococcus hominis, staphylococcus haemolyticus and staphylococcus saprophyticus, has been investigated. the reproducibility for species identification was 100% and the reproducibility for the single reactions was 95%. when compared to a modified kloos & schleifer conventional identification method, discrepant identification was obtained wit ...19948011304
prevalence of antibiotic resistance among clinical isolates of methicillin-resistant staphylococci.the prevalence of methicillin-resistant and multiply antibiotic-resistant staphylococci causing infections in hospitalized patients was studied over a two-year period. among 122 clinically significant staphylococci, the prevalence of methicillin resistance was 66%, with a higher prevalence of staphylococcus haemolyticus (85%) and staphylococcus epidermidis (83%) observed than of staphylococcus aureus (49%). multiple antibiotic resistance was observed more frequently among coagulase-negative stap ...19948013488
characterization of the most frequently encountered staphylococcus sp. in cats.ninety three staphylococci isolated from clinical specimens from cats were characterized and identified. because the biochemical characteristics of staphylococcus felis were very similar to those of staphylococcus simulans, results were submitted to numerical analysis and dna homology. forty-two isolates (45%) were identified as s. felis, and 4 isolates (4%) as s. simulans. the other species identified, in order of their frequency were, 12 staphylococcus aureus (13%), 9 staphylococcus intermediu ...19948042273
studies on constituents of medicinal plants. xviii. constituents of the leaves of clethra barbinervis sieb. et zucc. (1). 1994264188
international colloquium on basic health services in developing countries. antwerp, belgium, 8-10 december 1978. 199445072
early electrophysiologic changes in conduction block.fibrillation potentials and positive sharp waves were recorded in the paralyzed muscles of 31 patients with a conduction block of more than 14 days' duration. in 75% of the muscles, these effects were attributed to axonal damage in addition to the local demyelinating block, this conclusion being based on changes in the properties of the motor unit found electromyographically when recovery had taken place. in the remaining 25% of the muscles, the denervation-like activity was thought to be an eff ...1994263978
[presentation of pulmonary tuberculosis cases suspected of pulmonary cancer (author's transl)]. 1994502150
[share of dental assistant in practice hygiene (i)]. 1994284478
letter: osteoblastic repair of osteolytic tumor metastases following treatment. 1995162786
antibacterial activity of wy-49605 compared with those of six other oral agents and selection of disk content for disk diffusion susceptibility testing.the in vitro antimicrobial activity of an oral penem, wy-49605, was compared with those of six other oral antimicrobial agents against 598 bacterial isolates representing 51 different species. wy-49605 exhibited good activity against most gram-positive bacteria and members of the family enterobacteriaceae. it had little activity against nonfermenting gram-negative bacilli, enterobacter spp., serratia spp., enterococci, staphylococcus haemolyticus, and methicillin-resistant staphylococcus aureus. ...19957492088
in vitro activity of the semisynthetic glycopeptide amide mdl 63,246.the in vitro activity of the semisynthetic glycopeptide amide mdl 63,246 against 293 u.s. clinical isolates of gram-positive cocci was determined by the broth microdilution method. when compared with teicoplanin, mdl 63,246 had improved activity against staphylococcus epidermidis (mics that inhibited 90% strains tested [mic90s], 0.25 versus 8 micrograms/ml, respectively). staphylococcus haemolyticus (mic90s, 1 versus 32 micrograms/ml, respectively), and vana enterococcus faecium (mic90s, 32 vers ...19957492109
comparative antimicrobial activities of the penem wy-49605 (sun5555) against recent clinical isolates from five u.s. medical centers.the in vitro activity of wy-49605 (sun5555) (wy) was compared with those of cefaclor, cefixime, and amoxicillin-clavulanic acid against 2,958 consecutive clinical isolates from five medical centers and 402 respiratory pathogens from 18 other facilities. most members of the family enterobacteriaceae were inhibited by wy (mic at which 50% of the isolates are inhibited [mic50], < or = 2.0 micrograms/ml). mic90s of > or = 8.0 micrograms/ml were observed for enterobacter cloacae, serratia spp., and p ...19957492110
antistaphylococcal activity of cefdinir, a new oral third-generation cephalosporin, alone and in combination with other antibiotics, at supra- and sub-mic levels.cefdinir is one of the few oral third generation cephalosporins that shows useful activity against nosocomial gram-positive pathogens. for this reason the anti-staphylococcal potency of the new drug, alone or in combination with other drugs was further characterized. against penicillin-resistant, oxacillin-susceptible staphylococcus isolates, cefdinir demonstrated useful in-vitro activity. mic90 values (in mg/l) were 0.25 for staphylococcus aureus (30 strains), 0.06 for staphylococcus epidermidi ...19957768782
[nosocomial infection caused by multiresistant staphylococci in a neonatal and pediatric intensive care unit].we selected a paediatric population with a high risk of nosocomial infection formed by 116 newborns (42.8%) and 155 not newborns (57.2%) admitted into the icu of the giannina gaslini institute during the period 1-1-1992-30-9-1992; we compared it with a reference paediatric population studied in the same department during the period 1-1-1987-30-6-1988 formed by 310 newborns (44.3%) and 391 not newborns (55.7%). the purpose of this study is to evaluate the possible change in the incidence of colon ...19957610072
four-year prospective study of staph-ident system and conventional method for reference identification of staphylococcus, stomatococcus, and micrococcus spp..a 4-year prospective study compared the accuracy of the staph-ident system (biomérieux vitek, inc., hazelwood, mo.) with that of the reference procedure of the centers for disease control and prevention for the identification of staphylococcus species, stomatococcus mucilaginosus, and micrococcus species. the study compared the results from 1,106 cultures (500 eye cultures, 217 strains submitted for reference identification, and 389 known stock strains) representing 21 species of the family micr ...19957699074
staphylococcus haemolyticus contains two d-glutamic acid biosynthetic activities, a glutamate racemase and a d-amino acid transaminase.two d-glutamic acid biosynthetic activities, glutamate racemase and d-amino acid transaminase, have been described previously for bacteria. to date, no bacterial species has been reported to possess both activities. genetic complementation studies using escherichia coli wm335, a d-glutamic acid auxotroph, and cloned chromosomal dna fragments from staphylococcus haemolyticus revealed two distinct dna fragments containing open reading frames which, when present, allowed growth on medium without ex ...19957814322
in-vitro activity of three fluoroquinolones, cefotaxime and clindamycin against staphylococci.the in-vitro activity of three fluoroquinolones (ciprofloxacin, ofloxacin, temafloxacin) against 200 well-defined clinical isolates of staphylococci was investigated. the in-vitro activity of the fluoroquinolones tested was equal, with a strong indication of cross resistance. a clear distribution over two populations of different quinolone susceptibility--"naturally susceptible" and "naturally resistant" strains--could be found in penicillin-resistant, methicillin-susceptible staphylococcus aure ...19959810661
emergence of bacterial resistance to imipenem and ciprofloxacin in a university hospital.we have continuously monitored the in-vitro activities of imipenem and ciprofloxacin against large numbers of non-fastidious clinical isolates. after eight years of use, 97-100% of enterobacteriaceae and acinetobacter baumannii remained susceptible to imipenem, but susceptibility of pseudomonas aeruginosa declined from 100% to 91%. after six years of use, 94%-100% of enterobacteriaceae (except providencia stuartii) remained susceptible to ciprofloxacin but susceptibility of p. stuartii, a. bauma ...19958522464
cloning and characterization of a novel, plasmid-encoded trimethoprim-resistant dihydrofolate reductase from staphylococcus haemolyticus mur313.in recent years resistance to the antibacterial agent trimethoprim (tmp) has become more widespread, and several trimethoprim-resistant (tmpr) dihydrofolate reductases (dhfrs) have been described from gram-negative bacteria. in staphylococci, only one tmpr dhfr has been described, the type s1 dhfr, which is encoded by the dfra gene found on transposon tn4003. in order to investigate the coincidence of high-level tmp resistance and the presence of dfra, we analyzed the dnas from various tmpr stap ...19958540692
functional cloning of the dihydropteroate synthase gene of staphylococcus haemolyticus.a 1,7-kilobase fragment of the staphylococcus haemolyticus chromosome containing the dihydropteroate synthase gene has been cloned by complementation in a temperature-sensitive mutant of escherichia coli. the gene, designated folp, predicts a gene product of 29613 da which shares significant amino acid sequence identity with other known bacterial dihydropteroate synthases. analysis of the dna sequence upstream and downstream of folp identified two further, incomplete open reading frames, one of ...19958586264
methicillin-resistant staphylococcus haemolyticus on the hands of health care workers: a route of transmission or a source?we undertook a cross-sectional study of hand carriage and environmental contamination of methicillin-resistant coagulase-negative staphylococci on three wards of a single subspeciality surgical service. sixteen hand cultures from 15 health care workers and 32 environmental cultures were obtained. of 49 isolates, 35 (72%) were staphylococcus haemolyticus. this species comprised 14 of the 16 (87%) hand isolates and 21 of the 32 (66%) environmental isolates. using restriction length polymorphism of ...19958586788
characterization of coagulase-negative staphylococci causing nosocomial infections in preterm infants.the species spectrum, antibiotic susceptibility, and genomic profile of coagulase-negative staphylococci (cns) isolated from infected preterm infants were compared with those obtained in cns from nursery personnel. staphylococcus epidermidis was the predominant species in the 66 investigated preterm infants (171 isolates), accounting for 64% of all isolates. a high proportion of staphylococcus haemolyticus (32%) could be detected. in contrast to the results in patients, the spectrum in nursery p ...19958605898
antimicrobial activity of cs-940, a new trifluorinated quinolone.the antimicrobial activity of cs-940, a new trifluorinated quinolone drug, was tested against 761 clinical isolates. cs-940 activity against members of the family enterobacteriaceae was most similar to that of ciprofloxacin and ofloxacin, with a large range of mics inhibiting 90% of isolates tested (mic90s) of 0.015 to 16 micrograms/ml (median mic90, 0.06 micrograms/ml). cs-940 had greater activity than ciprofloxacin or ofloxacin when they were tested against acinetobacter spp. (mic90s, 0.03 mic ...19958619590
[introduction and development of an administrative information system for the hospitals of kiel university]. 1995162765
characterisation of a virus (hl23v) isolated from cultured acute myelogenous leukaemic cells. 1995176596
development and application of weighted criteria to assess stainless steel crown restorations on primary teeth. 19953327936
coagulase-negative staphylococci in danish blood cultures: species distribution and antibiotic susceptibility.the distribution and antibiotic susceptibility of coagulase-negative staphylococci (cons) isolated from blood cultures was examined in samples from hospitals covering most of denmark. a total of 499 cons isolates were detected in 477 blood cultures from 340 patients and speciated as staphylococcus epidermidis, 285; staphylococcus hominis, 61; staphylococcus haemolyticus, 43; staphylococcus warneri, 12; staphylococcus cohnii, 7; staphylococcus saprophyticus, 4; staphylococcus capitis, 2 and staph ...19968690885
carriage of staphylococcus haemolyticus among healthcare workers. 19968744517
peptidoglycan synthesis and structure in staphylococcus haemolyticus expressing increasing levels of resistance to glycopeptide antibiotics.the structures of cytoplasmic peptidoglycan precursor and mature peptidoglycan of an isogenic series of staphylococcus haemolyticus strains expressing increasing levels of resistance to the glycopeptide antibiotics teicoplanin and vancomycin (mics, 8 to 32 and 4 to 16 microg/ml, respectively) were determined. high-performance liquid chromatography, mass spectrometry, amino acid analysis, digestion by r39 d,d-carboxypeptidase, and n-terminal amino acid sequencing were utilized. udp-muramyl-tetrap ...19968755902
emergence of teicoplanin-resistant coagulase-negative staphylococci.over a period of 5 years we have recovered 32 clinical isolates of coagulase-negative staphylococci (cons) exhibiting either decreased levels of susceptibility or true resistance to teicoplanin (mics, 16 to 128 micrograms/ml); these isolates make up 0.55% of the total cons isolated by us. twenty-nine of the strains were also methicillin resistant, and all were susceptible to vancomycin. fourteen of the strains were staphylococcus epidermidis, fourteen were staphylococcus haemolyticus, and four w ...19968784585
ampicillin-sulbactam is effective in prevention and therapy of experimental endocarditis caused by beta-lactamase-producing coagulase-negative staphylococci.optimal strategies for the prophylaxis and therapy of endocarditis caused by oxacillin-resistant, coagulase-negative staphylococci in patients with native or prosthetic valvular heart disease are not well defined. we compared the in vivo efficacies of ampicillin-sulbactam-based regimens with those of vancomycin-based oxacillin-resistant, beta-lactamase-producing coagulase-negative staphylococcal isolate (staphylococcus haemolyticus se220). ampicillin-sulbactam (100 and 20 mg/kg of body weight, r ...19968787887
coagulase-negative staphylococci in air samples from operating theatres.the aim of our study was to recognize coagulase-negative staphylococci (cns) in the air of operating theatres. out of the identification of 449 isolates, the most frequent species were staphylococcus epidermidis and staphylococcus haemolyticus. the strains were adherent to glass in 52.3%. most of the s. epidermidis showed adherent growth, while the majority of the s. haemolyticus failed to adhere. the disk-diffusion antibiotic sensitivity tests showed great differences in sensitivity to penicill ...19968806942
dna gyrase gyra mutations in quinolone-resistant clinical isolates of staphylococcus haemolyticus. 19968849234
characterization of is1272, an insertion sequence-like element from staphylococcus haemolyticus.we have previously shown (g. l. archer, d. m. niemeyer, j. a. thanassi, and m. j. pucci, antimicrob. agents chemother. 38:447-454, 1994) that some methicillin-resistant staphylococcal isolates contain a partial deletion of the genes (mecr1 and meci) that regulate the transcription of the methicillin resistance structural gene (meca). when a fragment of dna inserted at the point of the mecr1 deletion was used as a probe, hybridization with multiple bands was detected for staphylococcus haemolytic ...19968849253
a particle agglutination assay for rapid identification of heparin binding to coagulase-negative staphylococci.the heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). the particle agglutination assay was validated by comparing results with the adhesion (percentage binding of adherent cells) of coagulase-negative staphylococcal strains to heparinised microtitration plates. of 38 d ...19968849700
[studies on coagulase negative staphylococci isolated from urine].this study was conducted to invesitigate the relative frequency of staphylocossus spp. and coagulase negative staphylococci (cns) isolates from urine at juntendo university hospital in tokyo. the frequency of staphylococci spp. was encountered in about 10% of the cases from 1984 to 1994 (except 1992 and 1993), and no significant annual changes were observed. the frequency of staphylococcus aureus has been gradually increasing from 1986. the relative frequency of cns (321 strains) from 1989 to 19 ...19968851391
[antimicrobial susceptibility of coagulase negative staphylococci isolated from urine].this study was conducted to investigate the antimicrobial susceptibilities of the strains of coagulase negative staphylococci (cns) isolates from urine at juntendo university hospital in tokyo from 1989 to 1994. the susceptibility testing were performed according to the broth dilution method standerdized by the japan society of chemotherapy. the following bacteria were tested; staphylococcus epidermidis (59 strains), staphylococcus haemolyticus (42 strains), staphylococcus saprophyticus (30 stra ...19968851392
plasmid analysis of 26 staphylococcal species by a rapid microscale technique.evaluate a plasmid typing technique for a diverse group of staphylococci.19968899445
different susceptibility of coagulase-positive and coagulase-negative staphylococci to ciprofloxacin.ciprofloxacin susceptibility was evaluated in 573 clinical staphylococcal isolates (staphylococcus aureus 230, methicillin-resistant (mr) 36%; coagulase-negative strains 343, mr 66%) collected from 1989 to 1995. resistance to ciprofloxacin for mr staphylococcus aureus was 25% until 1991 when ciprofloxacin was introduced into the hospital formulary, and rose progressively to 90% in 1994-1995. mr staphylococcus haemolyticus showed a ciprofloxacin resistance of 46% until 1991 and of 73% from 1992 t ...19968914131
an electron microscopic study of clinical and laboratory-derived strains of teicoplanin-resistant staphylococcus haemolyticus.staphylococcal resistance to glycopeptides (which involves more teicoplanin than vancomycin) is uncommon and largely confined to staphylococcus haemolyticus, an emerging nosocomial pathogen with a tendency to develop antibiotic resistance. in this study, six s. haemolyticus strains, including two isogenic pairs of teicoplanin-susceptible/-resistant strains and two resistant clinical isolates, were used in a morphologic and morphometric electron microscope investigation. cells from both clinical ...19969158766
aminoglycoside resistance among danish blood culture isolates of coagulase-negative staphylococci.a sample of 137 coagulase-negative staphylococci isolated from blood cultures in denmark over a 4-month period during 1992-1993 were tested for aminoglycoside resistance and for the presence of aminoglycoside-modifying enzymes. this was done on the basis of minimum inhibitory concentrations (mics) measured by agar dilution, inhibition zone diameter by disk diffusion, and dna dot blot analysis. using the national committee for clinical laboratory standards (nccls) mic breakpoints, 5%, 46%, 57%, a ...19969048865
elasmobranch rectal gland cell: autoradiographic localization of [3h]ouabain-sensitive na, k-atpase in rectal gland of dogfish, squalus acanthias.specific binding of radiolabeled inhibitor was employed to localize the na-pump sites (na,k-atpase) in rectal gland epithelium, a nacl-secreting osmoregulatory tissue which is particularly rich in pump sites. slices of gland tissue from spiny dogfish were incubated in suitable [3h]ouabain-containing media and then prepared for na,k-atpase assay, measurement of radiolabel binding, or quantitative freeze-dry autoradiography at the light microscope level. gross freezing or drying artifacts were exc ...1996229110
impotence in patients with chronic renal failure on dialysis: its frequency and etiology.fourteen patients with chronic renal failure and on a hemodialysis program underwent neurologic, psychiatric, and endocrine studies to determine the frequency and etiology of impotence in uremics. the data suggest that impotence is more frequent in the chronic dialysis group than in the general population. all seven of the impotent men were found to have prolonged nerve conduct on velocity and absent bulbocavernosus reflexes. several of the impotent men also had clinical depression and low plasm ...1997163773
breakthrough nosocomial bacteraemia due to teicoplanin-resistant staphylococcus haemolyticus in five patients with acute leukaemia. 19979049820
conjugative transfer of high-level mupirocin resistance from staphylococcus haemolyticus to other staphylococci.a conjugative plasmid, pxu10, encoding high-level mupirocin resistance was transferred from a staphylococcus haemolyticus isolate, cn216, to other coagulase-negative staphylococci and a restriction deficient staphylococcus aureus strain, xu21, but not to clinical isolates or a restriction-proficient laboratory strain (strain wbg541) of s. aureus. however, from xu21 it was cotransferred with a 3.5-kb chloramphenicol resistance plasmid to wbg541. the results demonstrated the ability of pxu10 to mo ...19979056015
the source of coagulase-negative staphylococci in the endophthalmitis vitrectomy study. a comparison of eyelid and intraocular isolates using pulsed-field gel electrophoresis.to determine the species distribution of coagulase-negative staphylococci (cons) in patients with endophthalmitis and to ascertain whether the patient's own flora was a major source of postoperative endophthalmitis following cataract extraction.19979076208
teicoplanin resistance in staphylococcus haemolyticus, developing during treatment. 19979096201
emergence of the trimethoprim resistance gene dfrd in listeria monocytogenes bm4293.the sequence of the trimethoprim resistance gene of the 3.7-kb plasmid (pip823) that confers high-level resistance (mic, 1,024 microg/ml) to listeria monocytogenes bm4293 was determined. the gene was identical to dfrd recently detected in staphylococcus haemolyticus mur313. the corresponding protein, s2dhfr, represents the second class of high-level trimethoprim-resistant dihydrofolate reductase identified in gram-positive bacteria. we propose that trimethoprim resistance in l. monocytogenes bm4 ...19979145882
compact growth of staphylococcus haemolyticus in soft agar is not due to hydrophobic interaction between the cocci.it was hypothesized that the formation of compact colony in soft-agar both in the presence and absence of serum, characteristic mainly for strains of the species staphylococcus haemolyticus among coagulase-negative staphylococci [szúcs et al. acta microbiologica hungarica 40:181-189 (1993)] was due to hydrophobic interaction between cocci. the effect of a number of surface active agents on this phenomenon was examined. neither 0.1% and 1%. tween-80 nor 5% and 10% ethylene glycol and polyethylene ...19979554167
multi-resistant staphylococcus haemolyticus in a neonatal unit in new delhi.we describe a cluster of infections in a neonatal nursery due to an infrequently reported staphylococcal species, staphylococcus haemolyticus. s. haemolyticus resistant to penicillin, methicillin, gentamicin, erythromycin, chloramphenicol and tetracycline (pmgect) was isolated from a series of infections in neonates (conjunctivitis 6, blood 2, pustules 2) over a period of 3 weeks in a neonatal nursery. surveillance cultures from 22 neonates, their mothers in an adjacent maternity ward and staff ...19979176572
an epidemiological study of blood culture isolates of coagulase-negative staphylococci demonstrating hospital-acquired infection.we applied pulsed-field gel electrophoresis (pfge) after smai digestion and random amplification of polymorphic dna (rapd) analysis with nine oligonucleotide primers to 146 blood culture isolates of staphylococcus epidermidis and 25 blood culture isolates of staphylococcus haemolyticus. these were obtained over a 12-month period from patients on the neonatal and hematology units of the central manchester health care trust. pfge demonstrated two clusters of isolates of s. epidermidis (type a and ...19979196185
adhesion of bacteria to stainless steel and silver-coated orthopedic external fixation pins.bacterial adhesion to silver-coated orthopedic external fixation pins was compared with stainless steel controls in an in vitro study. using five bacterial isolates from wound infections, the silver coating was found to reduce adhesion for escherichia coli, pseudomonas aeruginosa, and two strains of staphylococcus aureus while the converse applied for staphylococcus haemolyticus. when placed in human serum, both surfaces were conditioned to a similar extent with serum proteins; this conditioning ...19979260103
comparative in-vitro activities of quinupristin-dalfopristin against gram-positive bloodstream isolates.the in-vitro activity of quinupristin-dalfopristin was compared with those of vancomycin, teicoplanin, erythromycin, clarithromycin, rifampicin, imipenem, meropenem, ciprofloxacin and sparfloxacin against 414 bloodstream isolates of gram-positive cocci. quinupristin-dalfopristin inhibited strains of streptococcus pyogenes and streptococcus agalactiae at 0.12 mg/l, methicillin- and/or erythromycin-resistant staphylococcus aureus and staphylococcus epidermidis at 0.5 mg/l, staphylococcus haemolyti ...19979301986
antibacterial activity of quinolones against coagulase-negative staphylococci and the quinolone resistance-determining region of the gyra genes from six species.antibacterial activity of quinolones against three species of coagulase-negative staphylococci was investigated. tosufloxacin and sparfloxacin exhibited potent activities against staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus saprophyticus compared with other quinolones tested. from the analysis of the dna sequence in the quinolone resistance-determining region (qrdr), greater than 80% homology was recognized in coagulase-negative staphylococci. a series residue was c ...19979338491
cloning and expression of the glutamate racemase gene of bacillus pumilus.a glutamate racemase gene (muri) was found in bacillus pumilus cells and cloned into escherichia coli wm335, a d-glutamate auxotroph, by means of a genetic complement method. muri of b. pumilus encodes a 272-amino acid protein with an unusual initiation codon, ttg. the deduced amino acid sequence shows significant similarity with those of glutamate racemases from e. coli (ratio of identical residues, 28%), pediococcus pentosaceus (44%), and staphylococcus haemolyticus (49%). b. pumilus muri was ...19979354391
protease-induced multicell formation in staphylococcus haemolyticus.multicellular cells were efficiently induced in staphylococcus haemolyticus by the addition of protease to exponentially growing cultures at 30 c. electron microscopy revealed the formation of tetrad-shaped multicells that were septated but not separated from each other. incubation of the multicells with extract from the cells grown without protease resulted in a fourfold increase in the number of colony-forming units as compared with the untreated control. an electrophoretic analysis showed tha ...19979403505
avoparcin, a glycopeptide used in animal foods: antimicrobial spectrum and potency tested against human isolates from the united states.avoparcin is a glycopeptide antimicrobial that is widely used as a growth promoter in animal feeds in portions of western europe. recently, concern has emerged about the possible contribution of avoparcin use to the emergence of glycopeptide resistance in enterococci. relatively little information exists on the spectrum of activity and potency of avoparcin. we studied the activity of avoparcin compared to vancomycin, teicoplanin, and 3 other antimicrobials against 814 recent human clinical isola ...19979458981
pcr-based dna fingerprinting of staphylococcus haemolyticus to investigate nosocomial infections.objective: to apply pcr-based dna fingerprinting in a clinical microbiology laboratory to investigate nosocomial infections with staphylococcus haemolyticus. methods: dna fingerprints were generated by pcr on 99 s. haemolyticus isolates using different primer combinations based on eric, rep or arbitrarily chosen simple repeat sequences. results: primer combinations rep1+(gtc)6 and eric1+eric2 had sufficient discrimatory power and were chosen to analyze the clinical isolates. dna fingerprint patt ...199711864133
synergistic hemolytic activity of staphylococcus lugdunensis is mediated by three peptides encoded by a non-agr genetic locus.some strains of the coagulase-negative staphylococcus lugdunensis produce a synergistic hemolytic activity (slush), phenotypically similar to the delta-hemolysin of s. aureus. reverse-phase high-pressure liquid chromatography of supernatants from s. lugdunensis 307 yielded three late-eluting peaks of 3.5 kda with synergistic hemolytic activity. a degenerate oligonucleotide probe was designed from partial amino acid sequences of the 23-amino-acid (aa) tryptic fragments from one of the three peaks ...19978975897
neonatal airway colonization with gram-negative bacilli: association with severity of bronchopulmonary dysplasia.airway colonization with gram-negative bacilli (gnb) and gram-positive cocci (gpc) is common in mechanically ventilated neonates. whether gnb are related to nosocomial bloodstream infection (bsi) and/or to the severity of bronchopulmonary dysplasia (bpd) is unknown.19979002095
bacteremia due to teicoplanin-resistant and vancomycin-susceptible staphylococcus haemolyticus in seven patients with acute leukemia and neutropenia receiving prophylaxis with ofloxacin. 19979039542
activities of ciprofloxacin, levofloxacin, ofloxacin and sparfloxacin against speciated coagulase-negative staphylococci sensitive and resistant to fluoroquinolones.a total of 119 strains of coagulase-negative staphylococci isolated from clinical specimens were speciated and tested for sensitivity to methicillin and four fluoroquinolones (ciprofloxacin, sparfloxacin, levofloxacin and ofloxacin). resistance to fluoroquinolones was significantly more common in staphylococcus haemolyticus (43%) than in staphylococcus epidermidis (11%). methicillin-resistant strains of s. haemolyticus were more often resistant to ciprofloxacin than were methicillin-resistant st ...199718611829
induction of malignant transformation and mutagenesis by dihydrodiols derived from 7,12-dimethylbenz[a]anthracene. 1998105732
radiology societies. 1998104596
[action for children]. 1998107435
[histological dysplasias and cardiovascular pathology]. 1998105685
a second look at rheumatoid factor and other "autoantibodies". 199888899
[immunochemical determination of fetal hemoglobin]. 1998105745
[the behavior of bioelements during long-term parenteral feeding].26 adult patients were investigated for 26--35 postoperative days while being fed parenterally. serum levels and urine excretion of zinc, magnesium, calcium and phosphate were measured. from the results it can be assumed that only phosphate substitution is necessary during parenteral long-term nutrition.1998107121
[in vitro susceptibilities of clinical isolates of coagulase-negative staphylococci to glycopeptide antibiotics].two hundred and thirteen clinical strains of coagulase-negative staphylococci isolated in japan between 1980 and 1997 were analyzed for glycopeptide susceptibility by determining mic using both mueller-hinton agar (mha) and brain heart infusion agar (bhia) plates. of 37 staphylococcus epidermidis strains isolated between 1980 and 1981, all were susceptible to vancomycin and teicoplanin on both mha and bhia. however, of 122 isolates of staphylococcus epidermidis isolated between 1994 and 1997, 1 ...199810077772
[effectiveness of prolonged uterine relaxation by betamimetic drugs for threatened premature labour (author's transl)].it is statistically proven that long-term administered betamimetic drugs inhibit excessive uterine activity, but for the individual subject the extent of this effect can hardly be predicted. by studying 167 pregnancies treated for threatened premature labour it is confirmed that the success rate of the tocolytic treatment depends strictly on the clinical feature of the condition as observed on admission. by use of a simple scheme for classification of success, correlations with the importance of ...1998105481
problems in diagnosis and treatment of staphylococcus haemolyticus endocarditis in a hemodialysis patient. 199811864232
molecular characterization of epidemic multiresistant staphylococcus haemolyticus isolates.fifty-five staphylococcus haemolyticus specimens isolated from patients and neonatal intensive care unit staff were tested for susceptibility to 12 antimicrobial agents. there were 34 multidrug-resistant isolates which were resistant to oxacillin, ampicillin, cefazolin, cefmetazole, imipenem, and gentamicin. these isolates had a higher frequency of resistance to tobramicin and ofloxacin, and relatively high mics (2 to 4 micrograms/ml) for vancomycin, although none of the isolates were vancomycin ...19989884833
in vivo presence of capsular polysaccharide in coagulase-negative staphylococci of ovine origin.three strains of coagulase-negative staphylococci identified as staphilococcus warnery, staphylococcus simulans and staphylococcus haemolyticus were examine for evidence of capsular polysaccharide, in vitro by negative staining with india ink, and in vivo by transmission electron microscopy. in vitro, unstained materials surrounded clusters or single cocci. in vivo, capsula materials were surrounding phagocytosed bacteria cells. these capsular materials were either closely or loosely bound to ba ...19989497929
[bacterial strains isolated from neutropenic patients and their resistance to antibiotics].although gram negative as well as gram positive bacteria participate in febrile episodes of neutropenic patients, in particular recently the ratio of gram positive bacteria is increasing. the objective of the present work was to investigate the incidence and antibiotic resistance of pathogenic bacterial agents in neutropenic patients.19989511277
determination of the chromosomal relationship between meca and gyra in methicillin-resistant coagulase-negative staphylococci.meca, the gene that mediates methicillin resistance, and its accompanying mec locus dna, insert near the gyra gene in staphylococcus aureus. to investigate whether there is a similar relationship between meca and gyra in coagulase-negative staphylococci (cns), meca- and gyra-specific dna fragments were used to probe methicillin-resistant isolates of staphylococcus epidermidis (mrse) (n = 11) and staphylococcus haemolyticus (mrsh) (n = 11). the gyra probe hybridized to the same smai dna fragment ...19989527777
Displaying items 501 - 600 of 1723