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bacterial flora in inflammatory bowel disease.the pathogenesis of inflammatory bowel disease (ibd) involves an interaction between host susceptibility (which is partly genetically determined), mucosal immunity and the intestinal milieu. micro-organisms have physiological effects on mucosal structure, epithelial turnover, the intestinal immune cells and, thus, on many intestinal functions. toll-like receptors and nucleotide oligomerisation-binding domain proteins in host cells recognise specific bacterial molecules and modify the immune resp ...201020203504
association between faecalibacterium prausnitzii and dietary fibre in colonic fermentation in healthy human subjects.the intestinal microbiota are a complex ecosystem influencing the immunoregulation of the human host, providing protection from colonising pathogens and producing scfa as the main energy source of colonocytes. our objective was to investigate the effect of dietary fibre exclusion and supplementation on the intestinal microbiota and scfa concentrations. faecal samples were obtained from healthy volunteers before and after two 14 d periods of consuming formulated diets devoid or supplemented with ...201020346190
microbiota in pediatric inflammatory bowel disease.to test the hypothesis that compared with controls, children with inflammatory bowel disease (ibd) exhibit differences in the relationships between gut microbiota and disease activity.201020400104
quantification of faecalibacterium prausnitzii- and subdoligranulum variabile-like bacteria in the cecum of chickens by real-time pcr.the intestinal microbial community is playing an important role in health and production performance of chickens. to understand the effect on the intestinal microflora induced by various feeding strategies, feed additives, infections, and intestinal disorders, it is important to have methods for quantifying potentially important bacteria in the intestine. we describe a real-time quantitative assay for detection and quantification of a whole group of faecalibacterium prausnitzii and subdoligranul ...201020460669
the bacteriology of pouchitis: a molecular phylogenetic analysis using 16s rrna gene cloning and sequencing.to identify, compare, and contrast the microbiota in patients with and without pouchitis after restorative proctocolectomy (rpc) for ulcerative colitis (uc) and familial adenomatous polyposis (fap).201020562611
is the abundance of faecalibacterium prausnitzii relevant to crohn's disease?reports that bacteria within the firmicutes phylum, especially the species faecalibacterium prausnitzii, are less abundant in crohn's disease (cd) patients and supernatants from cultures of this bacterium are anti-inflammatory prompted the investigation of the possible correlations between the abundance of f. prausnitzii and the response to treatment in patients with gut diseases and healthy controls. in a randomized, double-blind trial, faeces were collected from healthy volunteers, and from pa ...201020695899
dysbiosis of fecal microbiota in crohn's disease patients as revealed by a custom phylogenetic microarray.a custom phylogenetic microarray composed of small subunit ribosomal rna probes, representing ≈500 bacterial species from the human and animal gut, was developed and evaluated for analysis of gut microbial diversity using fecal samples from healthy subjects and crohn's disease (cd) patients.201020848492
rifaximin modulates the colonic microbiota of patients with crohn's disease: an in vitro approach using a continuous culture colonic model system.rifaximin, a rifamycin derivative, has been reported to induce clinical remission of active crohn's disease (cd), a chronic inflammatory bowel disorder. in order to understand how rifaximin affects the colonic microbiota and its metabolism, an in vitro human colonic model system was used in this study.201020852272
the role of mucosal immunity and host genetics in defining intestinal commensal bacteria.dramatic advances in molecular characterization of the largely noncultivable enteric microbiota have facilitated better understanding of the composition of this complex ecosystem at broad phylogenetic levels. this review outlines current understanding of mechanisms by which commensal bacteria are controlled and shaped into functional communities by innate and adaptive immune responses, antimicrobial peptides produced by epithelial cells and host genetic factors.201020871399
differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.obesity alters gut microbiota ecology and associates with low-grade inflammation in humans. roux-en-y gastric bypass (rygb) surgery is one of the most efficient procedures for the treatment of morbid obesity resulting in drastic weight loss and improvement of metabolic and inflammatory status. we analyzed the impact of rygb on the modifications of gut microbiota and examined links with adaptations associated with this procedure.201020876719
[the human intestinal microbiota].the human intestinal microbiota constitutes a complex ecosystem which is now well recognized for its impact on human health and well-being. it contributes to maturation of the immune system and provides a direct barrier against colonization by pathogens. its possible implication in diseases of modern societies, currently increasing in prevalence, has been reported. these include allergies, inflammatory bowel diseases and possibly metabolic and degenerative disorders. the analysis of the molecula ...201020889008
[functional biostructure of colonic microbiota (central fermenting area, germinal stock area and separating mucus layer) in healthy subjects and patients with diarrhea treated with saccharomyces boulardii].the colonic content can be compared to a spatially structured high output bioreactor composed of three functionally different regions: a separating mucus layer, a germinal stock area, and a central fermenting area. the stool mirrors this structure and can be used for diagnosis in health and disease. in a first part, we introduce a novel method based on fluorescence in situ hybridization (fish) of sections of punched-out stool cylinders, which allows quantitatively monitor microbiota in the mucus ...201020889010
the role of bacteria in the pathogenesis of inflammatory bowel disease.crohn's disease (cd) and ulcerative colitis (uc) have features that suggest bacterial involvement, and all genetic models of inflammatory bowel disease (ibd) require the presence of commensal bacteria. cd is associated with innate immune response genes such as nod2/card15 and the autophagy genes atg16l1 and irgm. however, ibd responds to immunosuppression, suggesting that any bacteria involved are not acting as conventional pathogens. molecular techniques are rapidly advancing our knowledge of t ...201020981205
numerical ecology validates a biogeographical distribution and gender-based effect on mucosa-associated bacteria along the human colon.we applied constrained ordination numerical ecology methods to data produced with a human intestinal tract-specific phylogenetic microarray (the aus-hit chip) to examine the microbial diversity associated with matched biopsy tissue samples taken from the caecum, transverse colon, sigmoid colon and rectum of 10 healthy patients. consistent with previous studies, the profiles revealed a marked intersubject variability; however, the numerical ecology methods of analysis allowed the subtraction of t ...201021124491
[16s rrna gene-based molecular methods to monitor clostridium cluster iv community in the colon of piglets].to investigate the change of clostridium cluster iv community in the colon of piglets from 7 to 35 days of age, and its correlation with butyrate concentration.201021141473
diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-coa:acetate coa-transferase gene.butyrate-producing bacteria play an important role in the human colon, supplying energy to the gut epithelium and regulating host cell responses. in order to explore the diversity and culturability of this functional group, we designed degenerate primers to amplify butyryl-coa:acetate coa-transferase sequences from faecal samples provided by 10 healthy volunteers. eighty-eight per cent of amplified sequences showed >98% dna sequence identity to coa-transferases from cultured butyrate-producing b ...201019807780
quantitative differences in intestinal faecalibacterium prausnitzii in obese indian children.gut bacteria contribute to energy conservation in man through their ability to ferment unabsorbed carbohydrate. the present study examined the composition of predominant faecal microbiota in obese and non-obese children. the participants (n 28) aged 11-14 years provided fresh faecal samples and completed a dietary survey consisting of 24 h diet recall and a ffq of commonly used foods taken over the previous 3 months. faecal bacteria were quantitated by real-time pcr using primers targeted at 16s ...201019849869
liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates.a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ...20103417817
liquid chromatographic profiles of major carotenoid esters in commercially processed california navel and valencia orange juice concentrates.a procedure for establishing profiles of major carotenoid esters in commercially processed valencia and navel orange juice concentrates by reversed-phase liquid chromatography (lc) using sudan 1 as internal standard is described. the procedure involved conversion of 5,6-epoxides in heat concentrated citrus juices to more stable 5,8-epoxides by treatment of extracted carotenoids with hydrochloric acid followed by dual wavelength analyses at 400 and 465 nm using lc. the esters of 5,8-furanoids (au ...20103417817
salvage of angioaccess after late thrombosis of radiocephalic fistulas for hemodialysis.a retrospective study of 44 surgical procedures performed in 39 patients with late occlusions of radiocephalic fistulas for hemodialysis is reported. in 6 cases (13.6%) no apparent anatomical cause was found, and a simple thrombectomy was performed as a corrective procedure. some sort of arterial and/or venous stenosis was present in the other 38 cases. in 11 cases with evidence of mild localized vascular stenosis we performed a thrombectomy and then an endoluminal angioplasty with a balloon cat ...20103733357
morphological and metastatic murine melanoma variants: motility, adhesiveness, cell surface and in vivo properties.the behaviour in vivo of tight and loose variants of murine melanoma cells is further characterized. in vitro clonal morphology is reproduced on a variety of substrates. results suggest that repeated selection of loose cells can co-select for cells with high metastatic and colonization potentials. measurement of cell motility shows that 1g3 (loose) cells are more motile than 1g8 (tight) which are restricted to movements within clonal boundaries. studies of adhesive properties show that loose cel ...20103426920
new data on the acceptance of the hepatitis b vaccine by dental personnel in the united kingdom. 20102964251
p21-ras effector domain mutants constructed by "cassette" mutagenesis.a series of mutations encoding single-amino-acid substitutions within the v-rash effector domain were constructed, and the ability of the mutants to induce focal transformation of nih 3t3 cells was studied. the mutations, which spanned codons 32 to 40, were made by a "cassette" mutagenesis technique that involved replacing this portion of the v-rash effector domain with a linker carrying two bspmi sites in opposite orientations. since bspmi cleaves outside its recognition sequence, bspmi digesti ...20103062384
contact urticaria to chlorocresol. 20102948766
infective endocarditis and antibiotic prophylaxis failure following an endodontic procedure. 20103063597
infective endocarditis and antibiotic prophylaxis failure following an endodontic procedure. 20103063597
[end-to-side gastroduodenal anastomosis (author's transl)]. 20104618314
cancer cachexia.cancer cachexia describes a syndrome of progressive weight loss, anorexia, and persistent erosion of host body cell mass in response to a malignant growth. although often associated with preterminal patients bearing disseminated disease, cachexia may be present in the early stages of tumor growth before any signs or symptoms of malignancy. a decline in food intake relative to energy expenditure (which may be increased, normal, or decreased) is the fundamental physiologic derangement leading to c ...20103292798
[postoperative disorder of bladder emptying in gynecology: pathophysiology and possibilities for treatment].urinary retention after radical hysterectomy is due to partial or complete denervation of the bladder and proximal urethra which is dependent on surgical radicality. in contrast, incomplete bladder emptying after surgery for stress induced urinary incontinence, has its origin in an infravesicular functional obstruction and is dependent on the operation performed. the use of drugs (alpha-sympatholytics, parasympathomimetics) seem to be of limited value, especially after stress-incontinence surger ...20103063586
[postoperative disorder of bladder emptying in gynecology: pathophysiology and possibilities for treatment].urinary retention after radical hysterectomy is due to partial or complete denervation of the bladder and proximal urethra which is dependent on surgical radicality. in contrast, incomplete bladder emptying after surgery for stress induced urinary incontinence, has its origin in an infravesicular functional obstruction and is dependent on the operation performed. the use of drugs (alpha-sympatholytics, parasympathomimetics) seem to be of limited value, especially after stress-incontinence surger ...20103063586
[immunologic support for clinical bone marrow transplants]. 20103063589
[immunologic support for clinical bone marrow transplants]. 20103063589
lipid solubility of a series of drugs and its relevance to fatal poisoning.for 11 commonly used drugs, the n-octanol/water partition coefficient (ph 7.4 temperature 37 degrees c) and solubility in n-octanol were determined. the drugs tested were chlorpromazine, amitriptyline, trazodone, dextropropoxyphene, diltiazem, dibucaine, amethocaine, procaine, quinidine, acetylsalicylic acid and paracetamol. for eight of the drugs, the relative lipid saturation corresponding to a fatal plasma concentration was estimated from the two parameters determined above and the median fat ...20102897446
effect of salt depletion on sodium concentration in serum and urine of bufo chilensis. evidences for increased levels of neurohypophysial principles in their plasma.salt-depleted toads bufo chilensis were compared with animals maintained in nacl solution and a control group with respect to na+ content in serum and urine. plasma hydro-osmotic activity of the animals was measured by increased water transfer across the isolated urinary bladder of the frog (caudiverbera caudiverbera). sodium in serum is not affected by pre-adaptation in distilled water. urine na+ is markedly reduced. plasma from depleted animals increases water transfer across the isolated urin ...20102881665
relationship between hepatitis b surface antigen prevalence, per capita alcohol consumption and primary liver cancer death rate in 30 countries.the relationships between the prevalence of hepatitis b surface antigen (hbsag), mean annual per capita alcohol consumption and primary liver cancer (plc) death rates were explored in 30 countries. hbsag prevalence was associated, significantly, with the logarithm of the primary liver cancer death rate (simple correlation coefficient = 0.44, p less than 0.05). this significant association increased following adjustment for a country's mean annual per capita alcohol consumption (partial correlati ...20102843610
[bibliography of scientific papers of the charles' university medical school at hradec králové]. 20104620040
the physiology and pathophysiology of eicosanoids in the skin.eicosanoids may play a role in the pathophysiology of common inflammatory skin diseases such as psoriasis and atopic eczema. their involvement offers the hope for development of new antiinflammatory drugs for use in dermatopharmacology. on the other hand, new findings also suggest a tissue protective role of prostanoids in skin. in addition, physiological functions of lipoxygenase-derived eicosanoids are emerging, which may serve to maintain cutaneous integrity and restore normal skin structure ...20103152488
[selection and management of heart procurement]. 20113319508
[selection and management of heart procurement]. 20113319508
[clinical evaluation of bi-axial pendulum irradiation and pelvic structure tolerance in radiation therapy of carcinoma of the uterine cervix]. 20113237468
comparison of methods of carbamazepine dosage, individualization in epileptic patients.the aim of the present work was to analyse the predictive capacity of different optimization methods for carbamazepine dosage regimens according to population pharmacokinetic parameters and/or serum levels data. calculations were performed using a multiple non-linear regression program (multi 2 bayes) with two different options: (a) using the ordinary least squares methods and (b) the least squares method based on the bayesian algorithm. the predictive capacity of the three methods was analysed ...20113235481
comparison of methods of carbamazepine dosage, individualization in epileptic patients.the aim of the present work was to analyse the predictive capacity of different optimization methods for carbamazepine dosage regimens according to population pharmacokinetic parameters and/or serum levels data. calculations were performed using a multiple non-linear regression program (multi 2 bayes) with two different options: (a) using the ordinary least squares methods and (b) the least squares method based on the bayesian algorithm. the predictive capacity of the three methods was analysed ...20113235481
[scanning electron microscopy findings in brand new silicon posterior chamber lenses and those folded for implantation]. 20113209155
needle aspiration of nonperitonsillar head and neck abscesses. a six-year experience.twenty-five patients with various head and neck abscesses have been managed with needle aspiration as the initial surgical modality over a six-year period. twenty (83%) of 24 patients' abscesses resolved without formal surgical intervention. patient 25 in the series whose abscess, though initially resolving, reaccumulated within two weeks requiring an incision and drainage. recurrent cancer was then diagnosed. eight of ten large-volume abscesses (over 10 ml) were controlled with needle aspiratio ...20113166766
[comparison of the parameters of illness status in primary and intensive prenatal care]. 20113264973
[liver involvement in hodgkin's disease. histopathological study with clinico-biochemical correlation]. 20113187122
system characterization of a rotating gamma camera, optimized for single-photon emission computed tomography of the head using iodine 123 iodoamphetamine. 20113260502
system characterization of a rotating gamma camera, optimized for single-photon emission computed tomography of the head using iodine 123 iodoamphetamine. 20113260502
[surgical therapy of decubitus ulcers]. 20113374072
[surgical therapy of decubitus ulcers]. 20113374072
ileal ph in cystic fibrosis.in cystic fibrosis jejunal hyperacidity has an adverse effect on lipid digestion. it is not known whether hyperacidity extends to the ileum. we have studied ph through the whole length of the small bowel in 42 normal and 6 cystic fibrosis subjects by means of ph-sensitive radiotelemetry capsules. in cystic fibrosis subjects the time spent below ph 6.0 was significantly greater during the 1st h in the small bowel than in normal subjects. in the rest of the small bowel the ph remained above 6.0 in ...20113164499
trends in gastric cancer sex ratio in the united states.recent reports have suggested that the male/female ratio for gastric cancer has decreased over time and that the mean age of cases has increased. the authors calculated age- and sex-specific mortality rates for gastric cancer from 1950 through 1979 for whites using data from us vital statistics and population estimates from the us census bureau. whereas sex ratios based on crude mortality rates showed a decrease over time from 1.7 in 1950 to 1.5 in 1979, similar ratios based on age-adjusted mort ...20113815262
[stress incontinence in women; results of the stamey-pereyra surgical method of treatment]. 20114047215
[a case of cystic changes in a giant leiomyoblastoma of the stomach].a 44-year-old male was admitted to hospital with a complaint of abdominal distention. a ct scan revealed a giant multicystic mass in the abdominal cavity, a tumor that measured 34 cm in diameter, which had risen from the gastric body and grew in an exophytic fashion with a stalk. histologic findings exhibited a malignant leiomyoblastoma with cellular pleomorphism and a intracytoplasmic cystic change, which included disseminated nests of immature small round cells. after excisional surgery, the p ...20113204665
veterinary medicines in animal foods. 20113145058
[problems in realizing the results of medical research in practice]. 20113677150
damp housing and childhood asthma: validation of reporting of symptoms.the relations among parental reports of respiratory symptoms, bronchospasm measured after exercise, and the presence of visible fungal mould in the home was assessed in a population sample of 7 year old children (n = 873). wheeze in the past year was the symptom most closely associated with reported dampness and particularly with mould. the unadjusted odds ratio relating mould and wheeze was 3.70 (95% confidence interval 2.22 to 6.15), and after adjustment for housing tenure, number of people pe ...20113145060
hpa axis disturbance and treatment outcome in panic disorder.some patients with panic disorder exhibit an abnormal response to dexamethasone. as this phenomenon may reflect disturbances in the central noradrenergic system and as alprazolam may work through this system to produce improvement, we predicted a particularly robust response among nonsuppressors. fifty-two patients with panic disorder or with agoraphobia with panic attacks were given alprazolam as the sole treatment during either of 2, 8-week, double-blind, placebo-controlled trials. though base ...20113067754
[what happens to gallstone fragments? what clears the gallbladder after nonsurgical treatment of gallstones?]. 20113181010
[experiences with the preventive use of aminophylline (euphylline) in apnea and periodic respiration in infants].sleep apnea and periodic breathing in infants are the result of a mild hypoxia and they are the requirement for sids. as a results of the modern medicine each 4.-5. death is the cause of sids during the first year of life. the positive effect of theophylline on apnea of prematurity is known since 1973. we have given theophylline (euphyllin) from 1979 till 1986 to 198 premature infants with a birth weight below 2000 g for apnea prophylaxis orally in a doses of 9 mg/kg body weight per day distribu ...20113043316
acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum.acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls.201119926616
dysbiosis of the faecal microbiota in patients with crohn's disease and their unaffected relatives.a general dysbiosis of the intestinal microbiota has been established in patients with crohn's disease (cd), but a systematic characterisation of this dysbiosis is lacking. therefore the composition of the predominant faecal microbiota of patients with cd was studied in comparison with the predominant composition in unaffected controls. whether dysbiosis is present in relatives of patients cd was also examined.201121209126
randomised, double-blind, placebo-controlled trial of fructo-oligosaccharides in active crohn's disease.the commensal intestinal microbiota drive the inflammation associated with crohn's disease. however, bacteria such as bifidobacteria and faecalibacterium prausnitzii appear to be immunoregulatory. in healthy subjects the intestinal microbiota are influenced by prebiotic carbohydrates such as fructo-oligosaccharides (fos). preliminary data suggest that fos increase faecal bifidobacteria, induce immunoregulatory dendritic cell (dc) responses and reduce disease activity in patients with crohn's dis ...201121262918
intestinal microbiota was assessed in cirrhotic patients with hepatitis b virus infection. intestinal microbiota of hbv cirrhotic patients.to unravel the profile of intestinal microecological parameters in chinese patients with asymptomatic carriage of hepatitis b virus (hbv), chronic hepatitis b, decompensated hbv cirrhosis, and health controls and to establish their correlation with liver disease progression, we performed quantitative pcr and immunological techniques to investigate fecal parameters, including population of fecal predominant bacteria and the abundance of some virulence genes derived from escherichia coli, bacteroi ...201121286703
microbial dysbiosis in colorectal cancer (crc) patients.the composition of the human intestinal microbiota is linked to health status. the aim was to analyze the microbiota of normal and colon cancer patients in order to establish cancer-related dysbiosis.201121297998
bacteroides uniformis is a putative bacterial species associated with the degradation of the isoflavone genistein in human feces.inter-individual variation in isoflavone absorption depends on gut microbial degradation and affects the efficacy of these compounds. we hypothesized that inter-individual variation in fecal isoflavone disappearance coincided with variation in bacterial species. in vitro anaerobic fecal disappearance of isoflavones was measured from 33 participants by hplc. fecal microbial 16s rrna variable region pcr products were obtained from 4 participants with the greatest and least genistein or glycitein d ...201121525249
diversity of the autochthonous colonic microbiota.a longstanding hypothesis in intestinal microbial ecology is that autochthonous microbes (resident) play a role that is distinct from allochthonous microbes (transient microbes in the fecal stream). a challenge has been to identify this pool of microbes. we used laser capture microdissection to collect microbes from the mouse ascending colon. this area contains transverse folds that mimic human intestinal folds and contains a distinct population of intestinal microbes that is associated with the ...201121637026
in vitro fermentation of linear and {alpha}-1,2-branched dextrans by the human fecal microbiota.the role of structure and molecular weight in fermentation selectivity in linear +¦-1,6 dextrans and dextrans with +¦-1,2 branching was investigated. fermentation by gut bacteria was determined in anaerobic, ph-controlled fecal batch cultures after 36 h. inulin (1%, wt/vol), which is a known prebiotic, was used as a control. samples were obtained at 0, 10, 24, and 36 h of fermentation for bacterial enumeration by fluorescent in situ hybridization and short-chain fatty acid analyses. the gas prod ...201121666027
bacteria as trigger for chronic gastrointestinal disorders.apart from acute infections, microorganisms may also induce or perpetuate chronic inflammatory diseases and reversible or irreversible proliferation of various cells in the gastrointestinal tract (the extreme being adenocarcinoma and lymphoma). helicobacter pylori is not only involved in the pathogenesis of lymphoma and gastric adenocarcinoma. the steps and mechanisms of the carcinogenic process involve host predisposition, environmental factors, and strain virulence. the steps of lymphoma genes ...201121734380
identification and characterization of potential performance-related gut microbiotas in broiler chickens across various feeding trials.three broiler feeding trials were investigated in order to identify gut bacteria consistently linked with improvements in bird performance as measured by feed efficiency. trials were done in various geographic locations and varied in diet composition, broiler breed, and bird age. gut microbial communities were investigated using microbial profiling. eight common performance-linked operational taxonomic units (otus) were identified within both the ilea (180, 492, and 564-566) and ceca (140-142, 2 ...201121742925
Diversity of the autochthonous colonic microbiota.A longstanding hypothesis in intestinal microbial ecology is that autochthonous microbes (resident) play a role that is distinct from allochthonous microbes (transient microbes in the fecal stream). A challenge has been to identify this pool of microbes. We used laser capture microdissection to collect microbes from the mouse ascending colon. This area contains transverse folds that mimic human intestinal folds and contains a distinct population of intestinal microbes that is associated with the ...201121694499
highlighting new phylogenetic specificities of crohn's disease microbiota.recent studies suggest that gastrointestinal (gi) microbes play a part in the pathogenesis of crohn's disease (cd).201120722058
Influence of dietary blueberry and broccoli on cecal microbiota activity and colon morphology in mdr1a(-/-) mice, a model of inflammatory bowel diseases.OBJECTIVE: Enteric microbiota has been shown to be associated with various pathological conditions such as inflammatory bowel disease (IBD). This study aimed to determine the anti-inflammatory colonic effects of blueberries and broccoli in mdr1a(-/-) mice (IBD mouse model) through modification of microbiota composition in the gastrointestinal tract. METHODS: The mdr1a(-/-) mice were fed either a control diet or the control diet supplemented with either 10% blueberry or broccoli for 21 wk. We inv ...201122113065
high-level dietary fibre up-regulates colonic fermentation and relative abundance of saccharolytic bacteria within the human faecal microbiota in vitro.background: health authorities around the world advise citizens to increase their intake of foods rich in dietary fibre because of its inverse association with chronic disease. however, a few studies have measured the impact of increasing mixed dietary fibres directly on the composition of the human gut microbiota. aims of the study: we studied the impact of high-level mixed dietary fibre intake on the human faec ...201121952691
Decreased colonization of fecal Clostridium coccoides/Eubacterium rectale species from ulcerative colitis patients in an in vitro dynamic gut model with mucin environment.The mucus layer in the colon, acting as a barrier to prevent invasion of pathogens, is thinner and discontinuous in patients with ulcerative colitis (UC). A recent developed in vitro dynamic gut model, the M-SHIME, was used to compare long-term colonization of the mucin layer by the microbiota from six healthy volunteers (HV) and six UC patients and thus distinguish the mucin adhered from the luminal microbiota. Although under the same nutritional conditions, short-chain fatty acid production by ...201122092917
smokers with active crohn's disease have a clinically relevant dysbiosis of the gastrointestinal microbiota.background: patients with crohn's disease (cd) have an intestinal dysbiosis with components of the microbiota exerting differential immune effects. smoking is associated with an increased incidence of cd, more frequent relapse, and greater burden of surgery. this study aimed to investigate the association between smoking and the intestinal microbiota in patients with active cd. methods: patients with active cd (n = 103) and healthy controls (n = 66) were recruited and demographic and clinical da ...201122102318
common biostructure of the colonic microbiota in neuroendocrine tumors and crohn's disease and the effect of therapy.background: the aims were to comparatively investigate the biostructure of colonic microbiota in patients with neuroendocrine tumors and crohn's disease (cd) and to study the response of the microbiota to therapy. methods: sections of fecal cylinders from 66 patients with neuroendocrine tumors (net; 25 foregut, 30 midgut, 11 hindgut), 50 patients with cd (crohn's disease activity index [cdai] ≥150), and 30 patients with chronic idiopathic diarrhea seen at the charité hospital and 25 healthy cont ...201122113988
faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern india.the effect of vegetarian diets on faecal microbiota has been explored largely through culture-based techniques. the present study compared the faecal microbiota of vegetarian and omnivorous young women in southern india. faecal samples were obtained from thirty-two lacto-vegetarian and twenty-four omnivorous young adult women from a similar social and economic background. macronutrient intake and anthropometric data were collected. faecal microbiota of interest was quantified by real-time pcr wi ...201122182464
relationship between human intestinal dendritic cells, gut microbiota, and disease activity in crohn's disease.altered intestinal dendritic cell (dc) function underlies dysregulated t-cell responses to bacteria in crohn's disease (cd) but it is unclear whether composition of the intestinal microbiota impacts local dc function. we assessed the relationship between dc function with disease activity and intestinal microbiota in patients with cd.201121910165
Changes in Human Fecal Microbiota Due to Chemotherapy Analyzed by TaqMan-PCR, 454 Sequencing and PCR-DGGE Fingerprinting.We investigated whether chemotherapy with the presence or absence of antibiotics against different kinds of cancer changed the gastrointestinal microbiota.201122194876
[fine needle biopsy of the pancreas with computerized tomography control].the results of 96 computed tomography guided percutaneous pancreatic aspiration biopsies were reviewed and compared with the findings at laparotomy or autopsy or with a follow-up of at least one year. in only four instances the follow-up was inadequate and in 29 cases the specimen did not allow a cytologic or histologic diagnosis. of the remaining 67 biopsies 65 were diagnosed correctly and only 2 incorrectly. this corresponds to a sensitivity of 67.7% and a specificity of 97%. in patients with ...20113232439
[assessment of the functional potentials of the body in adapting to different altitudes]. 20113227675
organized nursing declares zero tolerance for rct. 20113167923
radiological demonstration of healing in paget's disease of bone treated with apd.twenty-three patients with paget's disease received the bisphosphonate apd and were examined radiologically every 6 months. because routine roentgenographic procedures were followed, a number of the x rays were not fit for comparison. the radiographic technique, as well as the positioning of the patients, is critical since both could lead to artefacts. all patients reached normal biochemical levels, usually within 6 months. out of the 23 patients 11 showed definite radiological improvement in on ...20113842289
[lipoidosis of the glomerular mesangium with accumulation of cholesterol. a novel hereditary disease].a new hereditary disease, transmitted autosomal-dominant, which affects the kidneys has been observed in four members of the same family, three males and a woman. no specific clinical manifestations of the disease have been established. a slight proteinuria was first revealed at the age of 17 years. after about twenty years of moderate proteinuria and microscopic hematuria terminal renal failure and severe arterial hypertension occur. specific for the disease is the storage of lipids, mainly cho ...20113237300
[sound injuries after surgery of otosclerosis].surgery for otosclerosis means that the sound protection due to the fixation of the footplate does not exist anymore. as the corti hair cells have been fragilized by proteolytic enzymes, it is absolutely necessary to reconstruct the sound mechanism in order to maintain the vibration of the perilymph absolutely within acoustical physiologic limit of movement. the means to realize this reconstruction are presented in this paper, as well as the results.20113059938
characterization of radiation resistant hypoxic cell subpopulations in kht sarcomas. (ii). cell sorting.hypoxic cells in kht sarcomas were characterized using fluorescence activated cell sorting based on the diffusion properties of the fluorochrome hoechst 33342. tumour-bearing female c3h/hej mice were injected i.v. with 10 micrograms g-1 hoechst 33342 and the cells derived from the tumours sorted on the basis of their staining intensities. for each sorted fraction the dna histogram was evaluated using fcm analysis. the results indicated that the bright and dim cells were not equally distributed a ...20113179180
[being a fan of delegated matters (aleksandr vasil'evich borisovskiĭ)]. 20113064403
use of inotropic and chronotropic agents in neonates.the determinants of cardiovascular function include preload, myocardial contractility, and afterload. myocardial contractility and afterload can be altered pharmacologically and form the basis for the clinical management of shock with inotropic and chronotropic drugs. there are extensive data from experiments in laboratory animals that the immature cardiovascular system may respond differently to these inotropic and chronotropic agents than the mature cardiovascular system. digitalis, isoprotere ...20113322631
obesity in children. 20113257638
cancer and interleukin-2. 20113260362
cancer and interleukin-2. 20113260362
penetration of nadolol into aqueous humour after a single oral dose.nadolol 20 mg was administered orally as a single-blind, single dose to nine patients about to undergo cataract extraction. intraocular pressures fell by a mean of 24% 3 h after administration. during the operation, aqueous humour and serum samples were taken for measurement of nadolol concentrations. aqueous nadolol concentrations ranged from 3.8 to 13.4 ng ml-1, and correlated with the serum drug concentrations (r = 0.84). the fall in intraocular pressure did not correlate with either the aque ...20113203065
food allergy. 20113202279
antibiotic associated henoch-schonlein purpura syndrome. 20113256349
synthesis of some derivatives of 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(piperazin-1-yl)-3-quino linecarbo xylic acid (norfloxacin) as potential antibacterial agents.twelve new fluoroquinolones, structurally related to norfloxacin, have been synthesized in order to investigate the effect of substituents at the secondary nitrogen of the piperazine ring on antimicrobial activity. the new substances (carbamates, isoureas, guanidines, ureas and cyanamides) tested on a variety of gram-positive and gram-negative organisms showed lower activity than the model compound.20113208891
[complications of retinoid treatment in children]. 20113165253
intercalary heterochromatin in drosophila. iii. homology between dna sequences from the y chromosome, bases of polytene chromosome limbs, and chromosome 4 of d. melanogaster.molecular and cytogenetic characteristics are given of a 2846 bp dna sequence from the ydm12 clone, previously derived from the long arm of the drosophila melanogaster y chromosome. sequence analysis revealed within it a 1176 bp fragment with 37 bp terminal inverted repeats, flanked by 6 bp direct repeats. this fragment (called "element 1360") appeared to be a-t rich, and was saturated with short direct and inverted repeats of different degrees of homology and consensus sequences for transcripti ...20113146482
a controlled trial of gamma-vinyl-gaba (vigabatrin) in drug-resistant epilepsy. 20113145776
hyponatremia and schizophrenia: a case presentation. 20113126424
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