Publications

repeated pulmonary thromboembolism in rabbits.	ten rabbits received 24 to 76 intravenous injections of finely divided thrombi prepared by a modification of chandler's apparatus. eight rabbits were killed soon after the last injection (group a) and two rabbits were allowed to survive for 4 months after the last introduction of thrombi (group b). ten rabbits received repeated saline injections only, and six animals underwent no manipulation whatever. six test rabbits and six control rabbits underwent catheterization of the pulmonary artery. th ...	1975	123612
clinical and microbiologic surveillance of neonatal staphylococcal disease. relationship to hexachlorophene whole-body bathing.	neonatal staphylococcal disease occurred in the nurseries of a university hospital three weeks after discontinuation of routine, daily, whole-body bathing of newborns with hexachlorophene. of 16 infants who had clinical manifestations within a two-week period, 11 cases were confirmed bacteriologically. shortly after onset of the outbreak, daily clinical and microbiologic surveillance and control measures on all infants and personnel were inaugurated. clinical surveillance after the outbreak did ...	1975	123704
[homologous transplants in the middle ear (author's transl)].	the original concept of tympanoplasty included besides the closure of the middle ear, the removal of pathological middle ear components leaving intact the functional and healthy components. the special conditions that prevail in the middle ear (aeration, absence of intrinsic trauma) allow the reconstruction of the middle ear mechanics to a great extent. animal experiments and clinical experience reveal good transplantation properties of denatured and preserved ear ossicles. they are partly repla ...	1975	123985
the mutagenicity of azathioprine in mice, drosophila melanogaster and neurospora crassa.	the chemotherapeutic coumpound azathioprine was tested for possible mutagenicity in swiss albino mice, drosophila melanogaster and neurospora crassa. utilizing the dominant-lethal assay it was found that acute oral doses of azathioprine (2 times 25 mg/kg body weight), induced dominant-lethal mutations in mouse spermatocytes. chronic oral doses of azathioprine (2 times 25 mg/kg body weight/week for 10 weeks) resulted in a greater rate of dominant-lethality. this increase was not permanent, and by ...	1975	124817
letter: bacteroides fragilis and spectinomycin.		1975	126220
amino-acid sequence of the cyanogen-bromide fragment from human plasminogen that forms the linkage between the plasmin chains.	the complete amino acid sequence of a cyanogen bromide fragment (122 residues) obtained from plasminogen is described. this fragment forms the overlap between heavy (a) and light (b) chains of human plasmin. the particular arginyl-valyl bond cleaved in the second step of the activation process is shown to be arg98-val99 in this fragment. this site is not very similar to the one in the nh2-terminal part of the molecule (arg68-met69). remarkable homologies with the 'triple loops' ('kringle structu ...	1975	126863
letter: susceptibility of bacteroides fragilis to spectinomycin.		1975	126984
effects of urinary proteins from certain leukemics upon macromolecular synthesis and enzyme levels in bone marrow cultures.	urinary proteins from human leukemic patients have been found to alter quantitatively macromolecular synthesis in primary mouse bone marrow cultures. urinary protein-stimulated incorporation of [3h]uridine into rna was found after 1 day of culture. increased levels of adenine phosphoribosyltransferase and lysozyme were demonstrable at 3 and 5 days, respectively, with urinary protein-supplemented cultures. the incorporation of 3h-labeled deoxynucleosides into dna was higher in the presence of uri ...	1975	126996
the possible role of tightly bound adenine nucleotides in oxidative and photosynthetic phosphorylation.	the tightly bound nucleotides of the beff-heart mitochondrial atpase are released during cold inactivation followed by ammonium sulfate precipitation. during incubation at 0 degrees c the sedimentation coefficient (s20w) of the atpase first declines from 12.1s to 9s. prolonged incubation or precipitation with ammonium sulfate leads to dissociation of the 9s component into subunits with s20w of 3.5s. the 9s component still bears bound nucleotides which exchange more extensively and rapidly with a ...	1975	127089
a comparative study of the effect of some steroid hormones on the response of rats to other drugs.	the effect of a series of steroid hormones on the pentetrazol convulsing action, hexobarbital narcotic action and hepatic drug metabolizing enzyme activities was determined in rats. all steroid compounds used antagonized the pentetrazol effect: the most potent was cortisone and the least potent testosterone. glucocorticoids and androgens shortened the hexobarbital sleeping time and increased the hepatic drug metabolizing enzyme activity. estradiol exhibited the opposite effect, whereas progester ...	1975	127182
calcium uptake and atpase activity of microsomes from heterotopically transplanted rabbit heart.	heterotopic cardiac transplantation was performed in rabbits. the calcium uptake and atpase activities of microsomal fraction were studied in three groups (group i, normal controls; group ii, early transplants; group iii, late transplants) during severe rejection as characterized histologically and electrocardiographically. group iii showed a significant decrease in calcium uptake but no difference in atpase activity compared with groups i or ii. the results demonstrate that in the rejecting tra ...	1975	127353
[pseudomonas aeruginosa--the causative agent of infection in birds].	pseudomonas aeruginosa was isolated from the viscera of diseased and dead birds (young and adult chickens) as well as from 9 poultry houses on seven farms. its typing was performed on the basis of the blue-green coloration of the cultures, their hemolytic activity, the pleasant odour of lime-tree, the growth in culturing at 42 degrees c, the presence of the pyocianin pigment, and the positive results of the cytochromoxidase test after gaby and hadley and the biochemical test after kovacs. the pa ...	1975	127412
variation of stemline karyotype in a hela cell line.	100 karyotypes of a hela cell line (modal number 69) were studied in details. it was observed that the stemline cells of this "triploid" somatic cell population showed high degree of chromosomal polymorphism. it has been discussed that the stemline cells have number of extra chromosomes (or parts of chromosomes) which are not essential for the genetic integrity of this cell population. a loss of some of these chromosomes (or their parts) does not induce any appreciable change in the genetic make ...	1975	127440
biologic activity of soluble collagen-anticollagen immune complexes as demonstrated by passive cutaneous anaphylaxis.	collagen-anticollagen immune complexes were prepared by gel filtration. complex formation was chemically and serologically demonstrated. complexes eluting in gammam- and gammag-peaks of antigen-antiserum mixture showed strong positive reactions in pca experiments performed with 20 guinea pigs. control preparations of gammam- and gammag-peaks of antiserum devoid of antigen gave significantly lower pca-response. the positive reaction of control preparations can be explained in part by a certain de ...	1975	127469
[androgen metabolism and leydig-cell function in acute and chronic liver disease (author's transl)].	diurnal variations of plasma testosterone and urinary excretion rates of testosterone-, androsterone-, aetiocholanolone-, and dha-glucuronide, as well as dha-sulphate were measured before and after selective leydig-cell stimulation (with 3 x 5000 iu human gonadotrophin) in eight patients with alcoholic liver cirrhosis, ten with acute hepatitis and four with haemochromatosis. the circadian variation of plasma testosterone and the maximal testosterone concentration after human gonadotrophin stimul ...	1975	127698
effect of essential phospholipids on the properties of atpases of isolated rat liver plasma membranes of young and old animals.	"essential" phospholipids (epl), rich in dilinoleyl-phosphatidylcholine were given orally to young and 780-800 day-old rats. the double-labelled (14c/32p) dilinoleyl-phosphatidylcholine was incorporated in plasma membranes and the amounts incorporated into different organs investigated. old animals incorporated higher radioactivity than young. the 14c/32p ratio however decreased in comparison with the substance administered. the amount of (na+-k+)-atpase in rat liver plasma membranes of old anim ...	1975	127894
[proceedings: the myocardial efficiency of the hypertonic heart--bases for evaluations and typical misinterpretations].		1975	128243
histochemistry and histoenzymology of the hydatid cyst (echinococcus granulosus batsch, 1786). ii. scolices and brood capsules.	scolices and brood capsules of healthy hydatid cysts from lungs of human patients were studied with histochemical and histoenzymatic methods. the subtegumental and flame cells were sepcially rich in glycogen, rna and some dehydrogenases such as sdh, mdh, nadh-reductase and g-6-pdh. the rostellar zone or invaginated pole, an area of marked contractile movements, showed intense activity in atp'ase and simple esterase. the so-called excretory pole shows strong activity in simple esterases, lipase, ...	1975	130750
congenital herpetic keratitis.	an infant, the first-born of twins, delivered by cesarean seciton without prior rupture of the membranes, was noted to have an advanced epithellal and stromal keratitis at berth. herpes simplex, type 1, was cultured form the cornea and from skin vesicles which developed subsequently. the advanced nature of the lesion indicates that it was acquired in utero. this appears to be the first reported case of congential herpetic keratitis.	1975	163075
purification and properties of stringent factor.	the stringent factor from escherichia coli is the product of the rela locus. it is the enzyme that catalyzes the synthesis of pppgpp and ppgpp eliciting a pyrophosphate transfer from atp to the 3'--oh of gtp (or gdp). this protein is responsible for the synthesis of pppgpp and ppgpp in stringent strains in response to an amino acid starvation. in vitro it catalyzes the synthesis of these guanosine compounds in either a ribosome-dependent reaction that requires a particular conformation of the ri ...	1975	163249
in vitro synthesis of dna in nuclei isolated from human lung cells infected with herpes simplex type ii virus.	an isolated nuclei system prepared from herpes type ii- and mock-infected human embryonic lung cells is able to synthesize cellular and viral dna in the same proportion as in vivo at various times after infection. incorporation of (3h)ttp in the in vitro reaction mixture requires mg2 plus and atp. overall in vitro dna synthesis in nuclei isolated from herpes-infected cells is semiconservative as demonstrated by bromodeoxyuridine-substituted dna density-transfer experiments, but exhibits a signif ...	1975	163358
a plasma inhibitor of the renin-antirenin reaction and the in vitro generation of angiotensin i.	in the course of studies designed to develop a radioimmunoassay system for the detection of renin, we have identified in human plasma a potent inhibitor that interferes with the renin-antirenin reaction. utilizing gel filtration, this renin-antirenin inhibitory activity was found to have the same molecular size as renin substrate. however, it could be separated from renin substrate by ion-exchange chromatography. when fractions containing this activity were tested in an in vitro system containin ...	1975	163425
induced agglutinability of 3t3 mouse fibroblasts.		1975	163446
equine infectious respiratory disease.	during the past 20 years the equine population of great britain and ireland has increased with the result that the practising veterinary surgeon is more frequently called upon to advise on equine problems. a significant portion of this advice is concerned with the examination of horses showing signs of this advice is concerned with the examination of horses showing signs of respiratory disease. numerous pathogens, which include viruses, bacteria, parasites and moulds invade the respiratory tract ...	1975	163516
cellulase location in cellvibrio fulvus.	the location of cellulase in c. fulvus depends on the carbon source for growth and the age of the culture. when cells were grown on glucose or cellobiose all cmc-hydrolyzing enzyme was cell-bound but only part of the activity was located on the cell surface. treatment of cells with edta, lysozyme, and detergents and subsequent fractionation experiments showed that cellulase was also located in the periplasm and bound to a membrane fraction. growth on cellulose gave cell-free cellulase active aga ...	1975	163668
3h-estradiol distribution in female, androgenized female, and male rats at 100 and 200 days of age.	tissue distribution of radioactivity was studied 48 h after gonadectomy and 1 h after iv injection of 3h-estradiol (1 mug/kg body wt) in 100- and 200-day-old normal female rats, female rats androgenized with 30, 100 or 1250 mug testosterone propionate (tp) at 5 days of age, and male rats. receptor-mediated uptake of estradiol, as shown by diethylstilbestrol (des) competition, was highest in preoptic area-anterior hypothalamus (poa-ah) and median eminence-basal hypothalamus (me-bh), but also occu ...	1975	163737
solubilization of a prostaglandin f2alpha receptor in bovine corpora lutea.		1975	163763
susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli.	susceptibility of 358 clinical isolates of obligate anaerobes to metronidazole was determined by an agar-dilution technique. only 66% of all isolates were inhibited by 6.25 mug/ml, whereas 30% required larger than or equal to 50 mug/ml. considerable variation in susceptibility was observed among different genera and species of bacteria. fusobacterium was most senstitive, followed by clostridium, bacteroides and peptococcus, peptostreptococcus, veillonella and acidaminococcus, and non-spore-formi ...	1975	163867
nongenetic complementation of group v temperature-sensitive mutants of vesicular stomatitis virus by uv-irradiated virus.	cells infected with temperature-sensitive (ts) mutants of complementation group v of vesicular stomatitis virus (vsv) give an enhanced yield at nonpermissive temperature when co-infected or superinfected with uv-irradiated virus. virions produced in these mixed infections are temperature sensitive and do not complement ts v45. rescue of group v mutants is multiplicity dependent. it can occur in the presence of cycloheximide; kinetics of rescue are similar in the absence or in the presence of the ...	1975	163919
some aspects of lipid metabolism in fatty liver and kidney syndrome in chicks.	various aspects of lipid metabolism were examined in broiler chicks affected with fatty liver and kidney syndrome (flks). plasma free fatty acid concentrations were invariably elevated. plasma triglyceride concentrations were increased amounts of triglyceride-rich lipoproteins. lipoprotein lipase activity in adipose tissue was considerably reduced, but in heart tissue the enzyme activity was increased. hepatic lipogenesis was reduced. rates of oxidation of palmitic and succinic acids by liver, h ...	1975	164057
lymphocyte adenyl cyclase activity in canine renal transplant rejection.		1975	164065
an effect of glucagon on 3', 5'-cyclic amp phosphodiesterase activity in isolated rat hepatocytes.		1975	164185
reversible hypothyroidism in growth hormone-deficient children treated with human growth hormone.	six children with human growth hormone (hgh) deficiency became hypothyroid during the course of their therapy with hgh. this was accompanied by a decreasing growth rate, clinical symptoms of hypothyroidism and decreased serum t4 concentrations. three of the 6 patients returned to the euthyroid state, both clinically and biochemically, with cessation of hgh therapy, and reinstitution of hgh precipitated hypothyroidism again in 2 of the three. the patients who remained hypothyroid have evidence of ...	1975	165211
thyroid-stimulating hormone and cyclic adenosine 3',5'-monophosphate in the regulation of thyroid gland function.	the initial step in tsh action reflects binding of the hormone to specific receptor sites on the plasma membrane. such binding has been studied using plasma membranes, homogenates, isolated thyroid cells grown in culture, and thyroid slices. 3-h- and iodinated tsh preparations have been used; the latter have been prepared using both chloramine-t and lactoperoxidase. some of the discrepancies reported in the literature might reflect the different thyroid and hormone preparations and the variable ...	1975	165359
antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis.	the susceptibility of 90 strains of bacteroides fragilis to 16 antibacterial drugs was determined by the agar plate dilution test and the disc diffusion test. good correlation of results was obtained with the two methods, and regression line analysis could be performed for beta-lactam antibiotics, erythromycin, tetracyclines, lincomycin and clindamycin. thus the standardized disc diffusion method can be used for susceptibility testing, but only a clear distinction between sensitive and resistant ...	1975	167431
(na+ + k+)-activated adenosinetriphosphatase: fluorimetric determination of the associated k+ -dependent 3-o-methylfluorescein phosphatase and its use for the assay of enzyme samples with low activities.		1975	167602
cytosine arabinoside- and interferon-mediated control of polyoma and sv40 genome expression.	by metabolic dna inhibitors such as arac, viral as well as host dna replication is suppressed in polyoma- and sv40-infected cells. the interruption of the current viral dna replication has no effect on the current transcription of the late viral genes. the persistence of the late transcription indicates that the onset, but not the persistence, of the viral dna replication is a prerequisite for the persistence of the late polyoma and sv40 genome transcription. pretreatment of monkey kidney cells ...	1975	169073
anaerobic infections.		1975	169694
comparative distribution of cytochrome oxidase, succinate permease, and fixed anionic sites on the intact inner mitochondrial membrane. polycationic ferritin as a visually detectable metabolic inhibitor.		1975	169743
kinetics of murine type c virus-specific dna synthesis newly infected cells.	replicating transforming functions of rauscher leukemia virus (rlv) and the rlv pseudotype of moloney sarcoma virus in mouse embryo fibroblasts were found to be most sensitive to inhibition by cytosine arabinoside (ara-c) 30 to 90 min after infection. the initiation of intracellular rlv dna synthesis was detected by nucleic acid hybridization within this time interval. treatment of infected cells with cytosine arabinoside abolished rlv dna synthesis. peak synthesis of the dna complementary to th ...	1975	170417
intraperitoneal oxygen and carbon dioxide tensions in experimental adhesion disease and peritonitis.	intraperitoneal oxygen and carbon dioxide tensions were studied in rats during silica-induced adhesion formation or fecal peritonitis. measurements of po2 and pco2 in the abdominal cavity were performed by means of an implanted silastic tonometer. during active adhesion formation one to three weeks after administration of silica, the intra-abdominal po2 decreased by 50 per cent from normal whereas the intra-abdominal pco2 and the rate of oxygen consumption in the peritoneum were elevated. progre ...	1975	170836
carcinogenesis in tissue culture 24: tumorigenicity and aggregate-forming capacity of mammalian cells in culture.	twenty two kinds of strains and substrains of mammalian cells were cultivated on a gyratory shaker to obtain cell aggregates. the relationship between the morphology of aggregates and tumorigenicity of the cells was investigated. in some groups of cells, the size of aggregates corresponded with their tumorigenicity, e.g. among rat liver cells untreated, hepatoma cells produced by back-transplantation of rat liver cells after the treatment with a chemical carcinogen in tissue culture, and the hep ...	1975	171466
narcotic dependence, narcotic action and dopamine receptors.		1975	171531
information contained in the amino acid sequence of the alpha1(i)-chain of collagen and its consequences upon the formation of the triple helix, of fibrils and crosslinks.	the molecule of type i collagen from skin consists of two alpha1(i)-chains and one alpha2-chain. the sequence of the entire alpha1-chain comprising 1052 residues is summarily presented and discussed. apart from the 279 residues of alpha1(i)-cb8 whose sequence has been established for rat skin collagen, all sequences have been determined for calf skin collagen. in order to facilitate sequence analysis, the alpha1-chain was cleaved into defined fragments by cyanogen bromide or hydroxylamine or lim ...	1975	171554
the epstein-barr virus and neoplasia.		1975	171578
central role for magnesium in coordinate control of metabolism and growth in animal cells.	the rate of dna synthesis in cultures of chicken embryo fibroblasts is reduced by deprivation of serum, high population density, and other "physiological" effectors, through a reduction in the number of cells in the s-period of the cell cycle. the same effect can be produced by drastically reducing the concentration of mg++ added to the medium. this effect is erratic, however, and better control of [mg++] can be achieved with phosphorylated compounds which preferentially bind mg++. both atp and ...	1975	171666
scanning electron microscopy of glucocrticoid-treated hepatocytes and hepatoma cells in culture.	the morphological effects of exposure to hydrocortisone have been examined in two cell lines of liver origin by scanning electon microscopy. in one of these, an aneuploid line derived from a morris hepatoma, the presence of hormone results not only in a suppression of cell proliferation, but in a marked flattening of the cells and loss of surface microvilli; in the other cell line, a diploid line derived from adult rat liver, the suppression of cell division is less marked, and the morphological ...	1975	171679
the kininase activity of ehrlichs' ascites solid tumor after treatment with oncolytic clostridia.	for the first time the effect of bacterial kininases of cl. oncolyticum m 55 atcc 13.732 could be demonstrated in the tumor itself. this is shown by an increased degradation of kinins by spore-treated tumor tissue in comparison with respective controls. the results indicate the influence of kininases on the capillary circulation of the tumor tissue as one of the fundamental reactions of oncolysis.	1975	171870
[mitosis and virus infection in cell cultures. ii. reaction of the nuclear apparatus of the cell to virus infection].		1975	171878
viral hepatitis: a pathologic spectrum.	acute viral hepatitis has several identifiable morphologic components but the major categories are (1) cytopathic, (2) inflammatory, and (3) regenerative. each category has independently variable characteristics. extreme alterations related to severity of disease, alteration of immune response, or pre-existing liver disease may result in diagnostic difficulties for the pathologist. in contrast to the usual concept, patients who survive fulminant viral hepatitis rarely, if ever, develop cirrhosis ...	1975	171949
unique bactericidal action of metronidazole against bacteroides fragilis and clostridium perfringens.	the comparative bactericidal activity of penicillin g, carbenicillin, clindamycin, and metronidazole against eight susceptible strains of bacteroides fragilis and four strains of clostridium perfringens was determined by performing colony counts anaerobically of cultures incubated in brucella broth. with the b. fragilis strains, there was a lag phase of growth of approximately 8 h, during which time metronidazole did not reduce the colony counts. however, within 4 h of the onset of exponential g ...	1975	172007
convenient non-chromatographic assays for the microbial deconjugation and 7alpha-oh bioconversion of taurocholate.	we described two convenient assay methods to estimate bile acid deconjugation and bile acid bioconversion at the 7alpha-oh position by individual microorganisms grown in media containing taurocholic acid. the methods are based on (i) a selective chemical assay for taurine conjugates previously described and (ii) the use of a cell-free preparation of 7alpha-hydroxysteroid dehydrogenase from escherichia coli to directly quantify 7alpha-oh groups. these non-chromatographic approaches have been appl ...	1975	172011
sedimentation behavior of fructose 1.6-bisphosphatase in crude tissue extracts of the rabbit and the rat.		1975	172019
effects of "natural oestrogen" replacement therapy on menopausal symptoms and blood clotting.	in a double-blind study on the value of equine ("natural") oestrogens 30 patients presenting with menopausal symptoms in a group practice were monitored for possible adverse effects on blood clotting, weight, and blood pressure. the women were randomly allocated to two groups and given either three months' hormone treatment followed by three months' placebo or vice versa. an appreciable amelioration of all symptoms on placebo made it difficult to asses the genuine value of oestrogen treatment du ...	1975	172181
letter: cytomegalovirus infection and exchange transfusion.		1975	172186
inhibition of replication of virus-transformed fibroblasts by antibodies to rna.	the activity of 7s immunoglobulins (ig) antibody to rna, obtained in rabbits after a prolonged immunization with rna-methylated bovine serum albumin complex was evaluated in vitro on normal (3t3) and simian virus 40-transformed (sv 3t3) mouse fibroblasts. the presence of anti-rna antibody in the culture medium inhibited both the sv 3t3 cell proliferation and the [3h]thymidine incorporation. in contrast, an increased [3h]uridine incorporation was evident within 48 and 96 hr of culture. no signifi ...	1975	172224
influence of formycin b on polyadenosine diphosphoribose synthesis in vitro and in vivo.	formycin b inhibited growth of l5178y mouse leukemia cells in concentrations of less than twice the concentration that inhibits cell proliferation at 50% by cytostasis; at higher concentrations (more than twice the 50% concentration mentioned), the cells were killed. in cells treated with the concentration that inhibits cell proliferation at 50%, the average cell volume did not change. the formycin b inhibitory effect on cell proliferation was reduced by coincubation with nicotinamide adenine di ...	1975	172231
[demonstration of antigen-antibody complex of tsv5c12 cell membranes by means of guinea pig fluorescent anti-complement antibodies].	the application of rabbit anti-guinea pig complement fluorescent antibody to sv40 transformed cells demonstrated the presence of immune complexes on the cell membrane surface. the presence of such complexes was not detectable by using fluorescent rabbit anti-hamster immunoglobulins antisera.	1975	172249
variant human diploid fibroblast clones able to grow in homocysteine in place of cyst(e)ine.		1975	172353
elevation of intracellular calcium ion concentration provokes production of 1,2-diacylglycerol and phosphatidate in human erythrocytes.		1975	172387
persistence of cmv genome in lymphoid cells after congenital infection.		1975	172796
differential effects of oxygen and oxidation-reduction potential on the multiplication of three species of anaerobic intestinal bacteria.	the sensitivity of three strains of anaerobic intestinal bacteria, clostridium perfringens, bacteroides fragilis, and peptococcus magnus, to the differential effects of oxygen and adverse oxidation-reduction potential was measured. the multiplication of the three organisms was inhibited in the presence of oxygen whether the medium was at a negative oxidation-reduction potential (eh of -50 mv), poised by the intermittent addition of dithiothreitol, or at a positive oxidation-reduction potential ( ...	1975	173238
effect of calcium on cyclic amp-dependent and cyclic gmp-dependent endogenous protein phosphorylation in mouse brain cytosol.		1975	173328
the consequences of lipid peroxidation in isolated hepatocytes.	lipid peroxidation was initiated by the addition of either adp-complexed fe3+ or cumene hydroperoxide to isolated rat hepatocytes and the resultant biochemical and morphological alterations investigated. as previously observed with microsomes, malonaldehyde formation was associated with the inactivation of glucose-6-phosphatase. inhibition of microsomal oxidative drug metabolism was correlated with the release and subsequent inactivation of nadph-cytochrome c reductase, whereas cytochrome p-450 ...	1975	173537
plasma levels of beta-msh and acth during acute stresses and metyrapone administration in man.	simultaneous measurements of both beta-melanocyte stimulating hormone (beta-msh) and adrenocorticotropic hormone (acth) in extracted plasma were performed by specific radioimmunoassays. during insulin-induced hypoglycemia, there was a marked increase of plasma acth levels and a slight but significant increase of plasma beta-msh levels. lysine-vasopressin on the other hand, caused a significant rise of plasma acth levels without corresponding response of plasma beta-msh. following glucagon admini ...	1975	173735
strand-specific transcription of polyoma virus dna-early in productive infection and in transformed cells.	the dna strand origin of nuclear and cytoplasmic polyoma-specific rna in productively infected mouse cells and in a line of polyoma-transformed hamster cells was determined by hybridization of unlabeled rna with radioactively labeled separated strands of polyoma dna. early in the productive cycle (10 h postinfection) nuclear viral rna is complementary to only about 40% of the e strand of viral cna. no rna complementary to the l strand was detected even when the rna was first self-annealed to enr ...	1975	173883
studies on the distribution of certain phosphatases in the alimentary canal of indian cattle leech, poecilobdella granulosa (savigny, 1822).		1975	174484
[enzyme alterations of the arterial wall following the combination of a lathyrogenic treatment and of moderate hypercholesterolemia in the young rabbit].	to determinate the part of humoural and parietal factors in atherosclerotic injury genesis, metabolism alteration study is realised on aortic cell wall of rabbits which are submitted to a chronical lathyritic intoxication alone, or simultaneously or alternatively associated with a cholesterolemic diet. catabolic activity increase of beta-glucuronidase occurs in hypercholesterolemic rabbits. beta-aminoproprionitrile, lathyrogenic drug used, stimulates biosynthetic pathways: increase of soluble pr ...	1975	174789
non-bacterial infections associated with neoplastic disease.		1975	176031
immunofluorescent studies on the multiplication of equine arteritis virus in vero and e. derm (nbl-6) cells.		1975	176497
epizootiology of natural infection with bovine leukemia virus.		1975	183681
experimental infection of marmoset and squirrel monkeys with attenuated hvs.		1975	183695
[effect of morphine in vitro on the oxidative phosphorylation in rat liver mitochondria].	the rates of respiration in the presence of adp and of phosphorylation as an atp-ase activity of rat liver mitochondria was inhibited was in vitro by morphine with ki=6.5 mm. the uncoupler-stimulated respiration of the mitochondria and the activity of atp-ase and synthesis of atp in the submitochondrial particles were not altered in the presence of morphine. it is suggested that morphine inhibited the adenine nucleotide transport through the mitochondrial membrane	1975	179644
steroid production by quartered and capsular rat adrenal gland in response to haemorrhage.	60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ...	1975	179800
effects of cholecalciferol on the translocation of calcium by non-everted chick ileum in vitro.	an apparatus is described that allows perfusion of a non-everted segment of intestine in vitro and the study of the accumulation of substances within the mucosal cells. the translocation of ca(2+) by rachitic-chick ileum and the effect of pretreatment with cholecalciferol was investigated, with the following conclusions. (1) entry of ca(2+) across the microvilli into mucosal cells is by diffusion; it does not require metabolic energy or the presence of any other inorganic ions. (2) pretreatment ...	1975	177000
lack of correlation between the presence of estrogen receptor and histological type of mammary cancer induced by 7,12-dimethylbenz[alpha]anthracene.	the relation between the histological type and the presence of estrogen receptor or response to oophorectomy was investigated in the mammary cancer induced by 7,12-dimethylbenz[alpha]anthracene in spargue-dawley rats. the estrogen receptor was present in 75.0% (3/4) of poorly differentiated cancer and in 62.5% (10/16) of well-differentiated cancer. the histological degree of differentiation or grading of the rat mammary cancer did not affect the presence of the estrogen receptor or response to o ...	1975	178560
[significance of changes in the oxidation-reduction processes in the pathogenesis of visceral shock].		1975	181721
effects of temperature on diphosphopyridine nucleotide-linked isocitrate dehydrogenase from bovine heart. aspects of the kinetics, stability, and quarternary structure of the enzyme.	a temperature-dependent conformational change of the active dpn-linked isocitrate dehydrogenase was observed. when initial reaction kinetic data were examined between 35 and 5 degrees, the hill number (n) varied from 2 at higher to n approaching unity at lower temperatures, with an inflection point at 17 degrees. the presence of manganous isocitrate in the incubation media shifted the transition temperature for enzyme inactivation by 5,5'-dithiobis(2-nitrobenzoate) from 8-16 degrees. these tempe ...	1975	234954
the esterase activity of bovine carbonic anhydrase b above ph 9. reversible and cooalent inhibition by acetozolamide.	the reversible complex between the metalloenzyme bovine carbonic anhydrase b and the sulfonamide inhibitor acetazolamide can be "frozen" irreversibly by the addition of a covalent bond between the methyl group of the inhibitor and the tau-nitrogen of histidine-64. in both cases the inhibited enzyme is inactive as an esterase toward p-nitrophenyl propionate at physiological ph but retains activity controlled by an ionization in the protein exhibiting a pk-a greater than 10. similarly, both the co ...	1975	235550
diuretics, urate excretion and sodium reabsorption: effect of acetazolamide and urinary alkalinization.	the uricosuric properties of acetazolamide were investigated in order to elucidate the relationship between changes in proximal tubular sodium reabsorption and urate excretion in man. acetazolamide produced a modest uricosuric response which was not suppressible by pyrazinamide. alkalinization of the urine with sodium bicarbonate elicited an even smaller increment in the urate clearance. if urinary alkalinization does play a role in the uricosuric response to acetazolamide, it probably decreases ...	1975	235746
multiple forms of 7-alpha-hydroxysteroid dehydrogenase in selected strains of bacteroides fragilis.	multiple forms of 7-alpha-hydroxysteroid dehydrogenase were detected in six of nine strains of bacteroides fragilis. the enzymes differed with respect to pyridine nucleotide specificity, thermal stability, divalent metal cation requirement, and elution profilies from sephadex g-200 columns. the nicotinamide adenine dinucleotide phosphate (nadp)-dependent enzyme required divalent metal cations, preferentially mn-2+ (km, 57 mum), for maximum catalytic activity. the nadp-dependent enzyme was labile ...	1975	236279
production of cell-bound antigens by bacteroides fragilis nctc 9343 in continuous culture.	highly active antigen preparations, as measured by precipitation and inhibition of haemaglutination, were obtained from bacteroides fragilis nctc 9343 grown in continuous culture. the activity varied slightly only with the glucose concentration of the medium and the dilution rate. ph had a greater influence on antigen production.	1975	236632
nad- and nadp-dependent 7alpha-hydroxysteroid dehydrogenases from bacteroides fragilis.	twenty strains of bacteroides fragilis were screened for hydroxysteroid oxidoreductase activity in cell-free preparations. eighteen strains were shown to contain nad-dependent 7alpha-hydroxysteroid dehydrogenase. sixteen of the strains containing the nad-dependent enzyme also contained nadp-depedent 7alpha-hydroxysteroid dehydrogenase, but invariably in lesser amounts. a strain particulary rich in both 7alpha-hydroxysteroid dehydrogenase activities was selected for further study. measurement of ...	1975	236764
acid-base changes and excitation-contraction coupling in rabbit myocardium. ii. effects on resting membrane potential, action potential characteristics and propagation velocity.	the effects of changes in acid-base parameters on the resting membrane potential, action potential characteristics and propagation velocity were studied in isolated rabbit papillary muscles. lowering extracellular ph from 7.4 to 6.7 in a bathing solution buffered with 10 mm histidine did not alter the resting membrane potential or action potential characteristics but casused slight reduction in propagation velocity. a parallel increase in hco3-minus concentration (up to 47 mm)and pco2 at a const ...	1975	238360
susceptibility of bacteroides fragilis to spectinomycin.		1975	240793
survival of anaerobic and aerobic bacteria on cotton swabs in three transport systems.	the capacity of aerobic and anaerobic bacteria to survive on cotton swabs placed into a dry gassed-out co2-filled tube (dgt), dry sterile aerobic tube (dat), and a tube containing a modified stuarts' transport medium (mst), was assessed. pseudomonas aeruginosa increased in numbers by 2 and 3 logs when stored in mst and dat, respectively. the viability of p. aeruginosa, although retarded when compared to msa and dat, was not adversely affected by the co2 environment in the dgt. the mst maintaine ...	1975	240870
a ligand-induced conformational change of yeast dipeptidase at physiological ph: kinetic consequences and possible regulatory significance.	the hydrolysis of dipeptides by purified yeast dipeptidase (ec 3.4.13.?) shows marked deviations from michaelis-menten kinetics over a wide range of ph. quite anomalous kinetics is observed between ph 6 and 7, indicating a drastic change in the enzyme's properties. a reasonable explanation is provided by the assumption of a conformational transition brought about either by ph shifts or, at a constant ph, by changes in the substrate concentration. the transition, which may have a half-life on the ...	1975	241695
microbial kinetics of drug action against gram-positive and gram-negative organisms. ii: effect of clindamycin on staphylococcus aureus and escherichia coli.	clindamycin-affected staphylococcus aureus cultures show biphasic steady-state generation curves. an initial (phase i) generation of the clindamycin-affected staph. aureus is followed by an ultimate (phase ii) generation at the same dose level. the phase i apparent generation rate constant is greater than the phase ii apparent generation rate constant and suggests the development of resistant staph. aureus mutants to clindamycin action after a finite period of drug-bacteria contact at any subcom ...	1975	241826
[report of "lethal factor" synthetized by bacillus cereus (author's transl)].	bacillus cereus growing in nutrition broth produces during logarithmic growth and in stationary phase a lethal factor extremely toxic for rodents. this toxin is only produced in the presence of oxygen and depends on bacterial replication. the highest titers of toxin are obtained using bacteria in a concentration of 10(7) to 10(9) per ml of nutrition broth. during regression phase of bacillus cereus the titer of toxin declines to zero. toxicity of the lethal factor becomes not altered in an alcal ...	1975	242167
resistance of pseudomonas aeruginosa to antimicrobial drugs.		1975	181789
herpesvirus infections in renal allograft recipients.	17 of 37 immunosuppressed renal allograft recipients developed clinical and/or serological evidence of herpes simplex virus infections, 7 of 83 developed a zoster rash, 85 of loo had evidence of cytomegalovirus infection and in 3 of 30 an active epstein-barr virus was seen.	1975	181840
susceptibility of anaerobic bacteria to carbenicillin, cefoxitin, and related drugs.	the agar dilution technique was used for determination of the bacteriostatic activity of carbenicillin, penicillin g, cefazolin, cephaloridine, cefoxitin, and cephalothin agaomst a variety of anaerobic bacteria. carbenicillin showed a high level of activity at a concentration of smaller than or equal to 100 mug/ml; only five of 123 strains of bacteroides fragilis, one strain of bifidobacterium eriksonii, and one strain of clostridium bifermentans were resistant to a concentration of larger than ...	1975	804023
immune responses in eight patients infected with bacteroides fragilis.	sera from eight patients with either septicemia or pyothorax caused by bacteroides fragilis were assayed for specific antibody by the agglutination, immunofluorescence, and agar gel diffusion techniques. antibodies were detected in the sera of all eight patients by all three serological tests employed; titers as high as 1:160 and 1:320 were detected by the agglutination test and the immunofluorescence technique, respectively, and all patients had precipitating antibody. failure of b. fragilis su ...	1975	805185
factors that may prevent transfer of anti-biotic resistance between gram-negative bacteria in the gut.	antibiotic-resistance transfer between populations of donor and recipient strains of escherichia coli was completely inhibited in broth by dense suspensions of bacteroides fragilis. comparable amounts of inert bacterial matter (for-molised suspensions of e. coli or b. fragilis), or smaller numbers of viable b. fragilis, streptococcus faecalis, staphylococcus albus, neisseria catarrhalis, or solutions of sodium taurocholate or glycocholate were only moderately inhibitory. anaeroboisis had no effe ...	1975	805244
polymicrobial etiology of acute pelvic inflammatory disease.	we studied 204 women with acute pelvic inflammatory disease to delineate further the causes of that illness. gonococci were recovered from 91. gonococcal pili antibody rose or fell significantly in 12 of 18 patients with positive cultures and only two of 19 who had negative cultures and smears for neisseria gonorrhaoea(p smaller than 0.005). n. gonorrhoeae was found in peritoneal exudate from eight of 21 patients with, and none of 33 without, cervical gonococcal infection. among patients with se ...	1975	806017
gram-negative pneumonia in hospitalized patients.		1975	808801
immunochemical characterization of the outer membrane complex of bacteroides fragilis subspecies fragilis.	the outer membrane of gram-negative bacteria is immunologically the most important structure of the cell because of its accessibility to host defense mechanisms. by electron micrographic studies, it has been shown that bacteroides fragilis has a cell envelope typical of gram-negative bacteria. the outer memberane of this envelope was found to consist of protein, two polysaccharides bound to lipid in various degrees, and loosely bound lipid. the protein component of the outer membrane had a disti ...	1975	810521
combined activity of clindamycin and gentamicin on bacteroides fragilis and other bacteria.		1975	812858
[salmonella infection and host reaction, with special reference to enzymatic and histochemical studies].		1975	765538
a simple method for rapid isolation and identification of bacteroides fragilis.		1975	1108844