Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| creatinine excretion rates for evaluation of kidney function in children. | a protein load protocol for evaluation of kidney function was tested in normal children and pediatric renal patients. an overnight, timed urine collection was used for calculation of the baseline creatinine clearance and creatinine excretion rate. one hour following ingestion of a standardized protein meal (baked chicken), a 2-3 h urine collection was begun. the post-protein meal changes in creatinine clearance showed considerable variation in both the normal children and those with renal disord ... | 1988 | 3153054 |
| the elastogenic effect of recombinant transforming growth factor-beta on porcine aortic smooth muscle cells. | transforming growth factor-beta, a peptide growth factor, is known to be a multifunctional regulator of cellular activity. the effect of this growth factor on extracellular matrix formation is well established, but its effects on elastin, a critical component of lung, skin, and blood vessels are unknown. in the present study, by use of an enzyme-linked immunoassay method, we found that transforming growth factor-beta strongly increased elastin production in cultured porcine aortic smooth muscle ... | 1988 | 3165637 |
| antibacterial action of powdered semiconductor on a serotype g streptococcus mutans (short communication). | 1988 | 3165714 | |
| a new approach to the quality control of steroid receptor assays. | lyophilized cytosols prepared from calf uterus and human breast tumor tissue are commonly used to assess the reliability of routine steroid receptor assays. however, preanalytical error (sample preparation, storage, homogenization) cannot be detected in this way. participating laboratories were asked to mail us all their receptor results obtained over a four-month interval, and to include some information about the patients involved (age, menopausal status, nodal status). after verifying the hom ... | 1988 | 3168219 |
| [specificity of first and second generation hiv-1 antibody screening tests]. | among 9867 sera tested between january and april 1987 by abbott htlv-iii eia (cell culture-derived antigen) 155 reacted repeatedly positive. 22% of these could not be confirmed, while 77% could be confirmed by other tests. the results of 2 sera remained equivocal. subsequently abbott recombinant htlv-iii eia was used and until august of the same year 184 out of 11508 sera gave reproducible positive results. 12% of these could not be confirmed, while 86% could be confirmed. the results of 3 sera ... | 1988 | 3209234 |
| [consequences of the composition of breast milk for the nutrition of underweight newborn infants. i. calcium and phosphorus]. | 1988 | 3236663 | |
| [gonorrhea, chlamydia infection, trichomonas vaginitis and candidiasis in drug addicts. second report]. | 1988 | 3253803 | |
| rocky mountain spotted fever. | the epidemiology, pathogenesis, clinical features, and treatment of rocky mountain spotted fever are reviewed. rocky mountain spotted fever is a severe infection caused by rickettsia rickettsii transmitted to man by various species of ticks. high-incidence areas exist in the southeast and south central united states. only 60-70% of patients with the disease report a history of tick bite or exposure to tick-infested areas. the disease is initially characterized by fever, headache, gastrointestina ... | 1988 | 3280217 |
| [immunodiagnosis of aids using preparations of the recombinant antigen of the surface protein of the htlv-iii virus]. | a technological scheme has been developed for purification of recombinant antigen of surface protein (asp) of the causative virus of aids from escherichia coli cells carrying plasmid pl2 which codes for the synthesis of a hybrid polypeptide consisting of phage lambda n protein (59 amino acid residues) and a fragment of sp (env) of hiv virus (569 a.r.). purification of asp is based on two separation principles: fractionation of polypeptides of bacterial lysates by preparative isoelectrofocusing i ... | 1988 | 3285593 |
| [study of the effect of myxovirus and adenovirus infections on the level of serum immunoglobulins in infants aged 1-36 months]. | 1988 | 211481 | |
| hepatitis b as an occupational risk for nephrology nurses and technicians. | 1988 | 259616 | |
| focal nodular hyperplasia of the liver--nucleographic and ultrasonic findings. | 1989 | 210191 | |
| twenty-six year old woman with premature ventricular contractions and diagnostic auscultatory and echocardiographic findings. | 1989 | 267325 | |
| characterization of antilipolytic action of polyamines in isolated rat adipocytes. | the interactions of polyamines with the lipolytic system were studied in isolated rat adipocytes. spermine, spermidine and putrescine significantly inhibited adenosine deaminase-stimulated lipolysis. an antilipolytic effect of spermine was detectable at a concentration of 0.25 mm (p less than 0.05). at a concentration of 10 mm all three polyamines inhibited the stimulated lipolysis by 50-60% (p less than 0.001). in addition, spermine enhanced the antilipolytic sensitivity of insulin. spermine (1 ... | 1989 | 2476118 |
| hemocyte monophenol oxidase activity in mosquitoes exposed to microfilariae of dirofilaria immitis. | monophenol oxidase (mpo) activity in hemocytes collected from aedes aegypti liverpool strain and aedes trivittatus intrathoracically inoculated with saline alone, inoculated with dirofilaria immitis microfilariae (mff), or from uninoculated mosquitoes was compared using a radiometric tyrosine hydroxylation assay. hemocyte mpo activity in mff-inoculated (= immune-activated) mosquitoes was significantly increased at 24 hr postinoculation (pi) in a. aegypti and at 6, 12, and 24 hr pi in a. trivitta ... | 1989 | 2493084 |
| ocular manifestations of rocky mountain spotted fever in dogs. | dogs were examined ophthalmoscopically to determine the prevalence and type of ocular lesions associated with naturally acquired rocky mountain spotted fever (rmsf). in a consecutive series of 11 dogs with serologically confirmed rmsf, 9 had ocular involvement reflecting various degrees of vascular damage, including subconjunctival hemorrhage, hyphema, anterior uveitis, iris stromal hemorrhage, retinal petechiae, and focal areas of retinal edema and perivascular inflammatory cell infiltrate. the ... | 1989 | 2494144 |
| identification of rickettsiae in cutaneous biopsy specimens from dogs with experimental rocky mountain spotted fever. | direct immunofluorescence reaction for rickettsia rickettsii was performed on formalin-fixed, paraffin-embedded cutaneous biopsy specimens collected from dogs with experimental rocky mountain spotted fever (rmsf). a technique of trypsin digestion of deparaffinized, rehydrated sections was successful in demonstrating discrete, immunofluorescent organisms in endothelia and adjacent vessel walls in the dermis. r rickettsii was identified only in grossly evident dermal lesions (macular rash or oral ... | 1989 | 2494323 |
| production of antibody to and cellular localization of erythrocyte-sensitizing substance from rickettsia rickettsii. | antibodies to rickettsia rickettsii erythrocyte-sensitizing substance (ess) were raised in rabbits by using a derivatized ess. the resulting antibodies reacted with r. rickettsii and cross-reacted with rickettsia conorii, a member of the spotted fever group rickettsiae, but did not react with rickettsia typhi, a member of the typhus group rickettsiae, legionella bozemanii, or proteus vulgaris ox19 or ox2. immunoblot analysis indicated that ess was present in more than one fraction and that the m ... | 1989 | 2497137 |
| cross-reaction of immune sera from patients with rickettsial diseases. | rickettsia rickettsii and rickettsia conorii are the causative agents of two common and serious diseases, rocky mountain spotted fever and mediterranean spotted fever, respectively. in patients naturally infected with either of these organisms, antibodies are produced which cross-react with antigens of the other so extensively that diagnostic tests usually cannot identify the causative agents. the results of this study indicate that serodiagnostic tests with antigen from one of these two organis ... | 1989 | 2501497 |
| rocky mountain spotted fever: a disease in need of microbiological concern. | rocky mountain spotted fever, a life-threatening tick-transmitted infection, is the most prevalent rickettsiosis in the united states. this zoonosis is firmly entrenched in the tick host, which maintains the rickettsiae in nature by transovarian transmission. although the incidence of disease fluctuates in various regions and nationwide, the problems of a deceptively difficult clinical diagnosis and little microbiologic diagnostic effort persist. many empiric antibiotic regimens lack antirickett ... | 1989 | 2504480 |
| infected ticks feeding on persons in areas endemic for lyme disease and rocky mountain spotted fever. | 1989 | 2507649 | |
| probe directed at a segment of rickettsia rickettsii rrna amplified with polymerase chain reaction. | in an effort to explore a sensitive taxon-specific detection system for bacteria, we sequenced the 16s rrna from two strains of rickettsia rickettsii, five other rickettsiae, and coxiella burnetti to find a probe site unique to r. rickettsii. we then synthesized a 16-mer that hybridized only to the rrna of r. rickettsii. using a primer complementary to a sequence found only in rickettsial rrna, we then generated a cdna. we amplified the probe site in a 180-base segment of the cdna by using the c ... | 1989 | 2512323 |
| detection of rickettsia rickettsii dna in clinical specimens by using polymerase chain reaction technology. | a polymerase chain reaction (pcr) procedure for detecting rickettsial dna was developed and shown to be specific for rickettsia rickettsii and r. conorii, the etiologic agents of rocky mountain spotted fever (rmsf) and boutonneuse fever, respectively. blood clots were obtained from nine confirmed rmsf patients and six controls and analyzed for the presence of rickettsial dna by the pcr method. a defined region of the rickettsial genome was successfully amplified from seven of the nine clinical s ... | 1989 | 2512328 |
| [comparison of in vitro susceptibilities of rickettsia prowazekii, r. rickettsii, r. sibirica and r. tsutsugamushi to antimicrobial agents]. | the in vitro activities of 16 antimicrobial agents against rickettsia prowazekii (breinl strain), r. rickettsii (bitterroot strain), r. sibirica (atcc no. vr151) and r. tsutsugamushi (gilliam, karp, kato, shimokoshi, kawasaki and kuroki strains) were determined by the cell culture method. tetracycline, demethylchlortetracycline, doxycycline, minocycline, chloramphenicol, kitasamycin and rifampicin were generally effective (mic, 0.005-0.78 micrograms/ml) to all strains tested. quinolones such as ... | 1989 | 2512394 |
| cloning, expression and sequence analysis of the gene encoding the 120 kd surface-exposed protein of rickettsia rickettsii. | rickettsia rickettsii (r strain) genomic dna was partially digested and cloned into the lambda expression vector gt11 generating a genomic clone bank. transformant plaques were screened with antisera generated against the 120 kilodalton protein of r. rickettsii to detect those phage expressing the recombinant protein. the gene encoding the 120 kd protein was localized to a 4.3 kilobase sphi-bamhi fragment from the recombinant phage and subcloned into puc18 and puc19. full-length expression of th ... | 1989 | 2515418 |
| in vitro susceptibilities of rickettsia rickettsii and rickettsia conorii to roxithromycin and pristinamycin. | in vitro susceptibilities of rickettsia rickettsii and rickettsia conorii to roxithromycin, pristinamycin, and the pristinamycin compounds, p1 and p2, were determined by a dye uptake assay and a plaque assay. the mics were 1 microgram/ml for roxithromycin, 2 micrograms/ml for pristinamycin, greater than 256 micrograms/ml for p1, and 2 micrograms/ml for p2. compounds p1 and p2 did not share synergetic activity. the toxicity of each compound was determined by a dye uptake assay. toxic concentratio ... | 1989 | 2515795 |
| comparison of antigenic reactivity between intracellular and extracellular rickettsiae of spotted fever group. | antigens of intracellular rickettsiae in vero cells persistently infected with japanese yh strain of the spotted fever group (rickettsia japonica sp. nov.) reacted in the immunofluorescence test with sera from patients to the same extent by igg antibody titration as did antigens of extracellular rickettsiae prepared from culture fluid of lytically infected bhk cells. on the other hand, igm antibody titers measured with intracellular rickettsiae in vero cells were significantly higher in some cas ... | 1989 | 2516664 |
| a serological survey of ehrlichia canis, ehrlichia equi, rickettsia rickettsii, and borrelia burgdorferi in dogs in oklahoma. | serum samples from 259 dogs were tested for antibodies to ehrlichia canis, ehrlichia equi, rickettsia rickettsii, and borrelia burgdorferi using the indirect fluorescent antibody test. the sera were obtained from submissions to the oklahoma animal disease diagnostic laboratory during a 14-month period from june 1986 through july 1987. the rate for positive antibody titers to e. canis was 53%, to e. equi was 33%, to r. rickettsii was 38%, and to b. burgdorferi was 18%. higher percentages of sera ... | 1989 | 2518693 |
| mechanisms and kinetics for platelet and neutrophil localization in immune complex nephritis. | we have previously reported that both neutrophils (pmns) and platelets mediate proteinuria in a model of subendothelial immune complex (ic) nephritis (gn) in the rat. in order to understand the interaction of pmns and platelets in this model, we quantitated the uptake of 111in-labelled platelets in glomeruli and correlated this with the number of pmns observed histologically at 10 and 30 minutes, 1, 4 and 24 hours following induction of gn. platelet accumulation was biphasic with a major peak at ... | 1989 | 2533296 |
| effects of trh on the immune system of newborn rats. | 1989 | 2561202 | |
| metabolic changes in glutathione and metallothionein in newborn rat liver. | metallothionein (mt) and glutathione (gsh) both contain 30% cysteine and they have distinct developmental profiles in perinatal rat liver. the metabolic relationships between these two cysteine pools were investigated in newborn rats under various experimental conditions. injection of 2-day-old rat pups with buthionine sulfoximine, phorone or diethylmaleate decreased hepatic gsh levels without any change in high basal levels of mt or zinc. similarly injection of l-oxothiazolidine carboxylate inc ... | 1989 | 2566683 |
| [entomologic situation regarding arbovirus circulation in the guajaibon area]. | the collections of culicidae made in the guajaibón area, north coast of havana province, are analyzed with arbovirologic purposes. the predominance of aedes scapularis in the human bait collections is confirmed. the possibility of arbovirus circulation in the area is suggested. | 1989 | 2577010 |
| some trematodes of freshwater fishes from north vietnam with a list of recorded endohelminths by fish hosts. | the present paper comprises a systematic survey of trematodes collected from 12 species of freshwater fishes from north vietnam (the red river near hanoi). altogether 16 trematode species (14 adults and 2 juvenile forms) have been recorded. these include: amurotrema dombrowskajae, platycladorchis microacetabularis, p. macroacetabularis, neocladorchis multilobularis, carassotrema koreanum, azygia hwangtsiyui, phyllodistomum clariasi, phyllodistomum sp., orientocreadium batrachoides, masenia colla ... | 1989 | 2583614 |
| the role of rt1.a major histocompatibility antigens in prolonging the survival of hepatic allografts in the rat. | 1989 | 2650272 | |
| effect of oxoplatinum and cbdca on renal functions in rats. | changes in renal function in rats after single-dose intravenous administration of cbdca (cis-diammine-1,1-cyclobutane dicarboxylate platinum(ii) and oxoplatinum [cis-dichlorodiammine-trans-dihydroxo-platinum(iv)] at a dose of 20 mg/kg were determined and compared. renal function was monitored by several determinations of effective renal plasma flow (erpf), glomerular filtration rate (grf), plasma creatinine and urea and urine beta 2-microglobulin. a significant reduction of erpf and grf and a si ... | 1989 | 2654665 |
| regional hemodynamic changes following hypovolemia in conscious rats. | regional hemodynamic responses to subcutaneous injection of hyperoncotic polyethylene glycol (peg) were assessed in long-evans (i.e., normal) and brattleboro [i.e., arginine vasopressin (avp)-deficient] rats with chronically implanted, pulsed doppler probes. the results were compared with saline-injected time controls. peg injection elicited an early (30 min) selective mesenteric vasoconstriction in long-evans rats; 4-5 h after peg the mesenteric vasoconstriction was greater in long-evans than i ... | 1989 | 2655481 |
| adrenalectomy decreases nerve growth factor in young adult rat hippocampus. | the effect of adrenalectomy on the level of nerve growth factor (ngf) in the hippocampus and on the distribution of choline acetyltransferase immunoreactivity in forebrain cholinergic neurons of developing rats was studied. biological and immunohistochemical determinations indicated that in 40-day-old rats, adrenalectomy reduced the ngf level in the hippocampus and the choline acetyltransferase immunoreactivity in the septal lateral bands. furthermore, autoradiographic studies showed that adrena ... | 1989 | 2664787 |
| biologically active casein peptides implicated in immunomodulation. | maternal milk should not only be considered as a nutrient, but also as a protecting agent against aggressions from the neonate's new environment. breast-feeding facilitates transmission of a passive immunity by multifunctional factors which have a direct effect on the neonate's resistance to bacterial and viral infections. among these factors are the main milk proteins, the caseins: during enzymic digestion of human and bovine caseins, immunomodulating peptides are released. corresponding synthe ... | 1989 | 2668358 |
| laboratory survey of fluoroquinolone activity. | fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ... | 1989 | 2672262 |
| rickettsioses of the spotted fever group around the world. | spotted fever group rickettsioses comprise rocky mountain spotted fever (rickettsia rickettsii), boutonneuse fever (r. conorii), north asian tick typhus (r. sibirica), queensland tick typhus (r. australis), rickettsialpox (r. akari), and oriental spotted fever (r. japonica). ticks or mites serve as the vector and reservoir hosts of the rickettsiae. these obligate intracellular bacteria invade vascular endothelial cells, which are damaged directly, causing increased vascular permeability. the ras ... | 1989 | 2677080 |
| [tumor metastasis and growth factors]. | massive investigations are being carried out on growth factors. growth factors are an important determinant for the formation of successive tumor metastasis at target organs. we review the findings, including our data, of growth factors in relation to tumor metastasis. | 1989 | 2679395 |
| interrelationships between digestive proteolytic activities and production and quantitation of toxins in pseudomembranous colitis induced by clostridium difficile in gnotobiotic mice. | clostridium difficile pathogenicity is related to in vivo production of toxins, and it is of great interest to detect toxins produced in biological samples. several reports have shown that proteases in stools interfere with immunological methods for quantitation of toxin a. the purpose of this work was to estimate the relationship between the proteases and the c. difficile toxins produced in a gnotobiotic mouse model of pseudomembranous cecitis. cecal proteolytic activities hydrolyzed toxin a, a ... | 1989 | 2680988 |
| placental transport of calcium and phosphorus in pigs. | the role of vitamin d in regulating placental transport of calcium and phosphate in pigs was studied using the "hannover pig strain" which suffers from pseudo-vitamin d-deficiency rickets, type i. sows and fetuses of normal phenotype (heterozygotes) and of homozygote phenotype which suffered from clinical symptoms of rickets were used. the homozygote animals are devoid of renal 25-cholecalciferol-1-hydroxylase. in the rachitic sows which normally depend on treatment with pharmacological doses of ... | 1989 | 2681666 |
| impairment of lymphocyte suppressive system in recent onset insulin-dependent diabetes mellitus. correlation with blood glucose and serum insulin levels. | in a previous study, we observed an impairment of the theophylline-induced suppressive system in recent onset iddm patients, and demonstrated also a correlation with metabolic derangement. the aim of this study was to better investigate the relationship between theophylline sensitivity (ths) and blood glucose/plasma insulin levels in recent onset iddm patients subjected to preprogrammed variations by an insulin/glucose clamp with artificial pancreas. eight patients were studied within 8 weeks fr ... | 1989 | 2694717 |
| biological and clinical evaluation of the transmandibular implant. | the transmandibular implant has been shown to be a successful clinical solution to prosthetic rehabilitation of patients with gross mandibular atrophy. aspects of the biocompatibility of the implant material including the component metals in the alloy have been tested in an animal model utilizing sheep. the initial adelaide results in the clinical multicentre trial are presented. these were generally excellent and the reasons for the few failures are presented. | 1989 | 2695025 |
| biological and clinical evaluation of the transmandibular implant. | the transmandibular implant has been shown to be a successful clinical solution to prosthetic rehabilitation of patients with gross mandibular atrophy. aspects of the biocompatibility of the implant material including the component metals in the alloy have been tested in an animal model utilizing sheep. the initial adelaide results in the clinical multicentre trial are presented. these were generally excellent and the reasons for the few failures are presented. | 1989 | 2695025 |
| [evaluation of the anti-influenza vaccine in students from basic high school]. | the anti-influenza vaccine was applied to a group of 217 students of "batalla del jigüe" secondary school which was compared to a group inoculated with the vaccine placebo with the view to evaluate the results of vaccine protection against the flue, as well as to determine its secondary effects in an adolescent population. there was a significant difference in the immunologic response in favor of the vaccine group as compared to controls for both strains of the antigenic contents of the vaccine ... | 1989 | 2701186 |
| absence of genetic linkage of charcot-marie-tooth disease (hmsn ia) with chromosome 1 gene markers. | we previously reported a large charcot-marie-tooth family not linked to the duffy blood group marker, supporting the existence of genetic heterogeneity in this neuropathy. in order to investigate the possibility of another disease locus on chromosome 1, we analyzed this family further, using dna polymorphisms of 6 genes. absence of linkage makes a second disease locus on chromosome 1 unlikely. | 1989 | 2725880 |
| quantitative study of the dorsal lateral geniculate nucleus of the rat during the postnatal development. | the chronology of dlgn development was studied in the rat from birth to 90 days postnatal (dpn). our results indicate the faster dlgn growth occurs from 1 dpn to 21 dpn. the neuronal number and density was calculated. no significant neuronal loss was detected in the period investigated but the present study shows evidence of an important decrease (of 75%) in the neuronal density between 7 dpn and 21 dpn. immediately the study of neuronal sizes and shapes was undertaken. approximately at 14 dpn t ... | 1989 | 2732462 |
| alternative routes for the establishment of surface polarity during compaction of the mouse embryo. | during the process of compaction, mouse 8-cell blastomeres flatten upon each other and polarize along an axis perpendicular to cell contacts. if the process of flattening is prevented, polarization can still occur, but does so in a lower proportion of cells than for control populations, and without the normal contact-directed orientation. we compared contact-directed and noncontact-directed processes to see if they involve common mechanisms. in nonflattened cells, surface polarization was favore ... | 1989 | 2744237 |
| phylogenetic diversity of the rickettsiae. | small subunit rrna sequences have been determined for representative strains of six species of the family rickettsiaceae: rickettsia rickettsii, rickettsia prowazekii, rickettsia typhi, coxiella burnetii, ehrlichia risticii, and wolbachia persica. the relationships among these sequences and those of other eubacteria show that all members of the family rickettsiaceae belong to the so-called purple bacterial phylum. the three representatives of the genus rickettsia form a tight monophyletic cluste ... | 1989 | 2753854 |
| [blood supply of skeletal muscles in reactive hyperemia]. | in 18 anesthetized cats, 60-sec occlusion of the m. gastrocnemius artery entailed a decrease in the muscle blood supply by 15-20 ml of the blood volume in the vessels whereas the post-occlusion increase in the blood flow restored the blood volume with 45% excess. the intravascular accumulation of the blood exerting a moderating effect upon the venous return while preserving the increased arterial inflow, can be regarded as an automatic way of limiting the hemodynamic excitation due to the postoc ... | 1989 | 2759286 |
| effects of idebenone on memory impairment induced in ischemic and embolization models of cerebrovascular disturbance in rats. | two rat models of memory impairment in passive avoidance learning induced by cerebrovascular disturbance, were established to estimate the effects of a cerebral metabolic enhancer, idebenone. transient and global cerebral ischemia in rats, produced by 4-vessel occlusion for 200 s immediately after the acquisition trial of passive avoidance learning, shortened the latencies in the retention test trial performed 24 h later. this retrograde amnesia was reversed significantly by idebenone administer ... | 1989 | 2764639 |
| morphologic and morphometric studies on tumor necrosis produced by radiotherapy, and hyperthermia singly and in combination. | the purpose of the present study was to evaluate the therapeutic effects of hyperthermia associated with radiotherapy on neoplasia. two transplanted experimental tumors (undifferentiated carcinoma of mouse breast and sarcoma 37) were used. a protocol was followed that included, for both models, four groups of animals: 1) control group; 2) radiotherapy group; 3) hyperthermia group; 4) radiotherapy associated with hyperthermia group. the animals were sacrificed after therapy according to a differe ... | 1989 | 2770309 |
| [chronic pyelonephritis in polycystic kidney]. | the characteristics of chronic pyelonephritis are studied in 37 patients out of a total of 53 patients with proved renal polycystosis. a group of 71 patients with chronic pyelonephritis selected at random are used as a control group. the frequency of chronic pyelonephritis among the patients with renal polycystosis is 69.8%. the difference between the mean age of the patients with renal polycystosis and chronic pyelonephritis and the patients with renal polycystosis without chronic pyelonephriti ... | 1989 | 2773465 |
| human aldehyde dehydrogenase: coenzyme binding studies. | the binding of nadh and nad+ to the human liver cytoplasmic, e1, and mitochondrial, e2, isozymes at ph 7.0 and 25 degrees c was studied by the nadh fluorescence enhancement technique, the sedimentation technique, and steady-state kinetics. the binding of radiolabeled [14c]nadh and [14c]nad+ to the e1 isozyme when measured by the sedimentation technique yielded linear scatchard plots with a dissociation constant of 17.6 microm for nadh and 21.4 microm for nad+ and a stoichiometry of ca. two coenz ... | 1989 | 2775711 |
| opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony. | 1989 | 2780954 | |
| the induction of protective immunity to leishmania major in the balb/c mouse by interleukin 4 treatment. | the ability of interleukin 4 (il4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established leishmania major infections in the balb/c mouse was studied. il4 had a therapeutic effect on l. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. adoptive transfer of splenic t cells, obtained from il4-tre ... | 1989 | 2786473 |
| the development of analytical models for human trypanosomiasis. | the idea of biological equilibria is presented and applied first to the case of single species populations. difference and differential descriptions of population changes over time are compared and contrasted. the complications that arise when describing interactions between species are then illustrated for the case of vector-borne diseases, such as malaria. a simple model for malaria is explored and later extended to the case of the african trypanosomiases where more than one vertebrate host sp ... | 1989 | 2802828 |
| steroidogenesis in liposomal system containing adrenal microsomal cytochrome p-450 electron transfer components. | purified adrenal microsomal p-450c21 and/or p-45017 alpha,lyase were incorporated with purified nadph-cytochrome-p-450 reductase into liposome membranes composed of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine at a molar ratio of 5:3:1. the rate dependences of reduction of liposomal p-450c21 in the fast phase as well as progesterone hydroxylation activities of p-450c21 and p-45017 alpha,lyase on the reductase concentration in the liposome membranes suggested that electron ... | 1989 | 2804111 |
| modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats. | the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ... | 1989 | 2812289 |
| modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats. | the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ... | 1989 | 2812289 |
| a vaccine for ovine cysticercosis. | 1989 | 2815974 | |
| a rapid fluorometric method for semiautomated determination of cytotoxicity and cellular proliferation of human tumor cell lines in microculture. | a fluorometric method for the determination of cellular growth and cytotoxicity of human tumor cell lines in 96-well microculture plates is described. the assay is based on the combined use of the dna-binding dye hoechst 33342 and the fluorogenic substrate fluorescein diacetate (fda). hoechst 33342 undergoes a dramatic enhancement of fluorescence when specifically intercalated with cellular dna, whereas the fda fluorescence is dependent on cellular hydrolysis of the non-fluorescent substrate int ... | 1989 | 2817793 |
| alterations of coronary blood flow and reserve with aging in fischer 344 rats. | to determine whether aging affects the coronary circulation, left and right ventricular coronary blood flow and vascular resistance at rest and after maximal vasodilation were measured by left atrial injection of radioactive microspheres in conscious, unrestrained male fischer 344 rats at 4, 12, and 20 mo of age. as a function of age, maximal coronary blood flow per 100 g of tissue decreased by 43% in the left ventricle at both 12 and 20 mo, whereas a 44 and a 47% reduction was found in the righ ... | 1989 | 2912199 |
| effect of reduced energy metabolism and reperfusion on the permeability and morphology of the capillaries of an isolated rete mirabile. | the effects of reduction in energy metabolism were explored in the eel rete mirabile, an organ composed predominantly of capillaries. in vitro experiments showed that glycolysis is the major pathway of energy production in this capillary tissue, and that iodoacetate, kcn, and low po2 in combination markedly reduce its atp generation. when in situ energy generation was inhibited by this combination during countercurrent perfusion of the arterial and venous capillaries of the rete, an approximate ... | 1989 | 2912596 |
| duration of ischemia is vital for collateral development: repeated brief coronary artery occlusions in conscious dogs. | the effects of two types of repeated transient coronary artery occlusions on collateral development were examined in chronically instrumented, conscious dogs. a 2-minute coronary occlusion at 32-minute intervals (group 1, n = 11) or a 15-second occlusion at 4-minute intervals (group 2, n = 7) were repeated day and night without interruption. in both groups, the total duration of coronary occlusions each day was the same (90 minutes). before and after repetitive occlusions of either group, effect ... | 1989 | 2912600 |
| synthesis and copper-dependent antimycoplasmal activity of 1-amino-3-(2-pyridyl)isoquinoline derivatives. 2. amidines. | in our search for new compounds with antimycoplasmal activity, a series of aromatic amidines derived from 1-amino-3-(2-pyridyl)isoquinoline (1) was synthesized. in the presence of 40 microm copper the most active compounds show growth inhibition of mycoplasma gallisepticum in the nanomolar range. these compounds are 3 times as active as tylosin, an antimycoplasmal therapeutic agent that is used in veterinary practice. in the presence of copper, amidines derived from 1 are 2-3 times more active t ... | 1989 | 2913309 |
| improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin. | as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ... | 1989 | 2914405 |
| potassium secretion by the cortical collecting tubule: effect of c1 gradients and ouabain. | in various epithelia k and cl transport are molecularly coupled and kcl cotransport is dependent on the na-k pump. the present study examines 1) the effect of a bath-to-lumen cl gradient on k secretion during active na transport and 2) the effect of basolateral ouabain on k secretion and na absorption in the presence and the absence of a bath-to-lumen cl gradient. under symmetrical conditions there was significant k secretion (jk = -24.0 +/- 3.9 pmol.mm-1.min-1) and cl secretion (jcl = -15.7 +/- ... | 1989 | 2916662 |
| promoter structure and expression of the 155-kda surface antigen gene of rickettsia rickettsii. | 1990 | 1696076 | |
| species-specific monoclonal antibodies to rickettsia japonica, a newly identified spotted fever group rickettsia. | a total of 192 hybridomas were developed from mice immunized with rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathogenic species of spotted fever group rickettsiae, rickettsia akari, rickettsia australis, rickettsia conorii, rickettsia rickettsii, and rickettsia sibrica. seven of the species-speci ... | 1990 | 1696280 |
| polypeptides constituting the antigenic basis for identification of rickettsia sibirica species by the standard serotyping method for spotted fever group rickettsiae. | the antigens of rickettsia sibirica, rickettsia rickettsii, and rickettsia conorii were examined by western immunoblots and the standard immunofluorescent serotyping assay with mouse anti-rickettsia sibirica serum that is used for determining the species identification of spotted fever group rickettsiae. serum was employed prior to absorption and after absorption with purified native or heated rickettsia sibirica. the unabsorbed antiserum, the antiserum absorbed with heated rickettsia sibirica a ... | 1990 | 1697727 |
| iloprost inhibits neutrophil-induced lung injury and neutrophil adherence to endothelial monolayers. | we hypothesized that iloprost, a long-acting prostacyclin analog, would inhibit neutrophil (pmn)-induced lung injury and decrease pmn adherence to vascular endothelium. human pmns infused into isolated buffer-perfused rat lungs subsequently stimulated with phorbol myristate acetate (pma) resulted in lung injury as assessed by the accumulation of [125i]bovine serum albumin (125i-bsa) in lung parenchyma and alveolar lavage fluid. addition of iloprost to the lung perfusate, prior to activation of t ... | 1990 | 1698399 |
| [rocky mountain spotted fever]. | after returning from a holiday in the usa a 24-year-old man fell ill with diarrhoea, high fever and marked rash including the palms of the hands and soles of the feet. when a history of a tick bite in the usa was elicited, a rickettsial infection was suspected. treatment with doxycycline, 100 mg twice daily, was instituted finally and the fever slowly resolved. the patient became completely well again within four weeks. serological tests confirmed the diagnosis of rocky mountain spotted fever. | 1990 | 2104798 |
| vascular permeability and coagulation during rickettsia rickettsii infection in dogs. | the vascular permeability of the ocular fundus, alterations in the coagulation system, and plasma concentrations of thromboxane b2 (txb2) and 6-keto-prostaglandin f1 alpha (6-keto-pgf1 alpha) were studied in dogs following intradermal inoculation with 5 x 10(5) tcid50 of rickettsia rickettsii. twenty-four to 48 hours after the onset of fever and rickettsemia, multifocal areas of retinal vasculitis were evident, which corresponded to areas of altered vascular permeability demonstrated by fluoresc ... | 1990 | 2105679 |
| serologic survey of selected zoonotic disease agents in black-tailed jack rabbits from western texas. | a serologic survey for the agents of rocky mountain spotted fever (rmsf) (rickettsia rickettsii), borrelia spp. including the causative agent for lyme disease (borrelia burgdorferi), and plague (yersinia pestis) was conducted on blood samples collected from 30 and 46 black-tailed jack rabbits (lepus californicus) from an urban environment in lubbock, texas (usa) during winter 1987 and the following spring 1988, respectively. antibody titers to the agents of rmsf and borreliosis were detected in ... | 1990 | 2106044 |
| a recombinant rickettsia conorii vaccine protects guinea pigs from experimental boutonneuse fever and rocky mountain spotted fever. | there are no vaccines against boutonneuse fever and rocky mountain spotted fever. previous studies have identified a rickettsia rickettsii surface protein as a vaccine candidate and shown that an antigenically related protein is present in r. conorii, which causes boutonneuse fever. the gene encoding the r. rickettsii protein has been cloned and expressed in escherichia coli. we confirmed by 7.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis of rickettsial lysates followed by immunob ... | 1990 | 2106490 |
| rapid immunoperoxidase demonstration of rickettsia rickettsii in fixed cutaneous specimens from patients with rocky mountain spotted fever. | immunofluorescence (if) of skin biopsies for detection of rickettsia rickettsii (rr) has proven useful as a rapid test for confirmation of rocky mountain spotted fever (rmsf). however, if lacks sensitivity, requires special equipment and training, and is difficult to interpret. the authors have developed an indirect avidin-biotin immunoperoxidase (ip) technique to detect rr in fixed and frozen tissue sections. the technique was evaluated on fixed cutaneous specimens from patients dying of rmsf a ... | 1990 | 2106778 |
| causative agent of spotted fever group rickettsiosis in japan. | since 1984, it has been known that spotted fever group rickettsiosis exists in japan. we isolated three strains of the causative rickettsiae, designated katayama, misaka, and abe, from patients with the disease and studied the characteristics of the isolates. nude mice and cyclophosphamide-treated mice died after infection with the isolates. however, infected normal mice recovered and acquired immunity. infected adult male guinea pigs had fever, a scrotal reaction, and seroconversion. the isolat ... | 1990 | 2108090 |
| diagnosis of acute typhus infection using the polymerase chain reaction. | the first use of the polymerase chain reaction (pcr) for the diagnosis of an acute rickettsial infection is described. a primer pair derived from the 17-kda antigen sequence of rickettsia rickettsii gave specific amplification of a 434-base pair dna fragment from the genome of rocky mountain spotted fever and endemic and epidemic typhus. the assay could detect as few as 30 rickettsiae. detection of pcr-amplified dna with a nonradioactive dna probe confirmed an acute infection with rickettsia pro ... | 1990 | 2108225 |
| secretion of tissue-type plasminogen activator and plasminogen activator inhibitor by rickettsia conorii- and rickettsia rickettsii-infected cultured endothelial cells. | hemostasis abnormalities have been described in patients with mediterranean spotted fever and rocky mountain spotted fever. evidence of the activation of the fibrinolytic system has been obtained in both diseases. after experimental rocky mountain spotted fever, an elevated level of fibrinogen was found in parallel with the activation of the fibrinolytic system and transient elevation of the tissue-type plasminogen activator. later protein is mainly synthesized by endothelial cells. the ability ... | 1990 | 2115024 |
| a potential protective role for thiols against cell injury caused by rickettsia rickettsii. | 1990 | 2116100 | |
| evidence supporting the hypothesis that rickettsial virulence factors determine the severity of spotted fever and typhus group infections. | 1990 | 2116102 | |
| possible typhus-group infection in new york state: presentation of four suspect cases. | epidemiologic investigations were recently conducted on four cases which were reported in new york state in 1986 and 1987, three of which were within one family. these included hospital chart reviews, case or family interviews, animal trappings, and ectoparasite surveys. serologic tests and immunoblots were performed on blood samples obtained from these patients. all four patients had acute febrile illnesses; two required hospitalization and one died. microimmunofluorescence test results using r ... | 1990 | 2116104 |
| the 17-kilodalton protein antigens of spotted fever and typhus group rickettsiae. | 1990 | 2116105 | |
| characterization of the 120-kda surface protein gene of rickettsia rickettsii (r strain). | 1990 | 2116108 | |
| detection of rickettsia rickettsii dna in clinical specimens by enzymatic amplification using polymerase chain reaction technology. | 1990 | 2116111 | |
| kinetics of igm and igg responses to experimental and naturally acquired rickettsia rickettsii infection in dogs. | the kinetics of specific igm and igg antibody response was characterized in four 9-month-old beagles after inoculation of 2 x 10(2) plaque-forming units (pfu) of sheila smith strain of rickettsia rickettsii. immunoglobulin m antibodies were first detected by indirect immunofluorescence on postinoculation (pi) day 9, peaked by pi day 20, and were no longer detectable by pi day 80. immunoglobulin g antibodies became detectable between pi days 22 and 28, peaked by pi day 42, and decreased gradually ... | 1990 | 2117411 |
| a protective protein antigen of rickettsia rickettsii has tandemly repeated, near-identical sequences. | the nucleotide sequence of a rickettsia rickettsii gene that encodes a high-molecular-mass surface antigen (190 kilodaltons), which elicits protective immunity, was determined. the 6,747-nucleotide gene coded for a 2,249-amino-acid protein with a calculated molecular weight of 224,321. a 3.8-kilobase psti fragment proximal to the 5' end of the gene was found to consist of 13 highly related tandem repeats which constituted over 40% of the coding region. the repeated sequences could be divided int ... | 1990 | 2117568 |
| hispid cotton rats (sigmodon hispidus) as a source for infecting immature dermacentor variabilis (acari: ixodidae) with rickettsia rickettsii. | hispid cotton rats (sigmodon hispidus say and ord) were susceptible to infection with rickettsia rickettsii wolbach under laboratory conditions and were capable of serving as sources for infecting ticks with rickettsiae. cotton rats developed rickettsemias that could be detected for as long as 6 h following intraperitoneal inoculation of 10(5) plaque-forming units (pfus) of r. rickettsii (morgan strain). an estimate of the median infectious dose (id50) was made by inoculating six groups (eight a ... | 1990 | 2117665 |
| [spotted fever group rickettsiosis on awaji island, japan]. | three cases of spotted fever group rickettsiosis occurring on awaji island, hyogo prefecture, the first reported in the kinki district, are described. the illness appeared from august to september in 1988. high-grade fever and papular erythema were observed in all cases. eschar, lymphadenopathy and hepatomegaly were observed in two-thirds of the cases, respectively. the antibody titers for proteus ox-2 by the weil-felix reaction were elevated, and immunofluorescence test with r. rickettsii of th ... | 1990 | 2119406 |
| nucleotide sequence of the p34 gene from rickettsia rickettsii. | 1990 | 2124682 | |
| reduction of drug accumulation and dna topoisomerase ii activity in acquired teniposide-resistant human cancer kb cell lines. | we have isolated stable teniposide (vm26)-resistant cell lines from human cancer kb cells by stepwise exposure to increasing doses of the drug. at each step, we have purified vm26-resistant cell lines. kb/vm-a, kb/vm-b, kb/vm-1, kb/vm-2, kb/vm-3, and kb/vm-4 showed 3-, 6-, 12-, 16-, 74-, and 95-fold higher resistance to vm26 than did kb. we have further characterized kb/vm-2 and kb/vm-4 which showed about 15- and 100-fold higher resistance to vm26 or etoposide (vp16) than did kb. both vm26-resis ... | 1990 | 2168282 |
| morphological aspects of inhibition of transformation of rat kidney cells infected with a temperature-sensitive mutant of the rous sarcoma virus by rat fibroblast interferon. | normal rat kidney cells (nrk) infected with a temperature-sensitive (ts) mutant of rous sarcoma virus (rsv) at the permissive temperature (33 degrees c) exhibit the morphological and growth characteristics of the transformed state. by the use of this system, it has been shown that the addition of rat fibroblast interferon to infected cells grown at a nonpermissive temperature (38 degrees c) prevents re-transformation when they are introduced to the permissive temperature. merely treating cells a ... | 1990 | 2168311 |
| diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity. | human ehrlichiosis, an acute febrile illness caused by ehrlichia canis or a closely related rickettsial organism, was first identified in 1986. from 1986 through 1988, sera from 85 patients demonstrated a fourfold rise or fall in antibody titer to e. canis. seven (22%) of 32 patients initially tested during the first week after onset of illness. 17 (68%) of 25 tested during the second week, and all 18 tested during the third week had titers that exceeded the minimum positive titer of greater tha ... | 1990 | 2192013 |
| partitioning of nucleoids. | 1990 | 2194249 | |
| [nmr-microscopic studies of the condylar cartilage of the temporomandibular joint]. | the objectifying of growth mechanisms of the tmj is very important for orthodontic diagnosis, treatment as well as clinical research. condylar cartilage of the tmj from neonatal pigs was investigated on in vivo preparations by nmr-microscopy. nmr-micro-pictures and histologic slide preparations of condylar cartilage were compared and discussed in connection with its implications for treatment with functional appliances. | 1990 | 2227743 |
| immunization of cattle (bos indicus x bos taurus) against hyalomma anatolicum anatolicum using antigens derived from tick salivary gland extracts. | studies were conducted on 41 cross-bred (bos taurus x bos indicus) calves which were less than 1 year old. three types of salivary gland antigens (sg ag) viz. sg ag-i (whole), sg ag-ii (supernatant) and sg ag-iii (sediment), prepared from the ixodid tick, hyalomma anatolicum anatolicum, were used for immunizing these calves. the calves were divided into five groups. the first three groups were immunized with sg ag-i, -ii and -iii with freund's complete adjuvant (fca), respectively. the fourth gr ... | 1990 | 2276870 |
| ca2+ transients are not required as signals for long-term neurite outgrowth from cultured sympathetic neurons. | a method for clamping cytosolic free ca2+ ([ca2+]i) in cultures of rat sympathetic neurons at or below resting levels for several days was devised to determine whether ca2+ signals are required for neurite outgrowth from neurons that depend on nerve growth factor (ngf) for their growth and survival. to control [ca2+]i, normal ca2+ influx was eliminated by titration of extracellular ca2+ with egta and reinstated through voltage-sensitive ca2+ channels. the rate of neurite outgrowth and the number ... | 1990 | 2324199 |