Publications

escherichia coli as cause of ecthyma gangrenosum.		1986	3511469
feasibility of same-day identification of members of the family vibrionaceae by the api 20e system.	sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ...	1986	3517061
immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique.	thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ...	1986	3519753
production of extracellular enzymes and cytotoxicity by vibrio vulnificus.	thirty-three strains of vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. strains of vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronidase, dnase, sulfatase, and hemolysin. no differences between clinical and environmental isolates were noted. although none of the enzymes appeared to correlate with the ability of th ...	1986	3522090
chitinase determinants of vibrio vulnificus: gene cloning and applications of a chitinase probe.	to initiate study of the genetic control of chitinolytic activity in vibrios, the chitobiase gene was isolated by cloning chromosomal dna prepared from vibrio vulnificus. chimeric plasmids were constructed from sau3a i partial digests of chromosomal dna by ligating 5 to 15-kilobase fragments into the bamhi site, i.e., in the tcr gene, of pbr322 (amr tcr). the resulting plasmids were transformed into escherichia coli dh1. chitobiase activity of the insert-bearing clones was detected by using a ch ...	1986	3524459
hemorrhagic bullae associated with vibrio vulnificus septicemia. report of two cases.	bullous lesions associated with vibrio vulnificus infection developed in two patients, both of whom had hepatic cirrhosis. one patient had a recent history of ingestion of raw oysters, while the other patient had recently exposed skin lacerations to sea water. both patients died within 24 hours of hospitalization, in spite of antibiotic treatment. vibrio vulnificus was isolated from blood and bullae in both patients. histologic examination of skin biopsy specimens revealed epidermal/dermal separ ...	1986	3524472
vibrio vulnificus septicemia: report of four cases.		1986	3564544
composition of major outer membrane proteins of vibrio vulnificus isolates: effect of different growth media and iron deficiency.	the outer membrane proteins of vibrio vulnificus including isolates from humans, seawater and an asari clam were examined by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis. a major outer membrane protein with an apparent molecular weight of 48,000 (48k protein) was common to all the strains grown in 3% nacl-nutrient broth; however this 48k protein was not produced in any of the strains grown in chemically defined medium. other major outer membrane proteins with molecular weights ...	1986	3724558
virulence of vibrio vulnificus strains from marine environments.	vibrio vulnificus strains isolated from geographically diverse marine sources were compared with clinical isolates for phenotype and in vitro and in vivo production of virulence factors. there were no differences between environmental and clinical strains on the basis of biochemical characteristics or antimicrobial susceptibility patterns. cytolysin and cytotoxin titers produced by environmental strains were generally comparable to those of clinical strains. of 29 environmental isolates tested, ...	1986	3729387
plasmid carriage in vibrio vulnificus and other lactose-fermenting marine vibrios.	a total of 42 clinical and environmental isolates of vibrio vulnificus were examined for plasmid carriage. of these, only five (12%) harbored plasmids, which were of various molecular weights. in contrast, 20 of 32 (62.5%) unidentified lactose-fermenting vibrio spp. were found to possess plasmids with masses of 2.1 to 150 megadaltons. in these isolates, multiple plasmids were common, with an average of 2.25 plasmids per plasmid-containing strain. attempts to demonstrate a correlation with the pl ...	1986	3729404
purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins.	the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ...	1986	3730359
preparation, isolation and characterization of mouse ige monoclonal antibodies against trichosanthin protein.		1986	3739501
a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle.	serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ...	1986	3753338
calmodulin gene expression during sea urchin development: persistence of a prevalent maternal protein.	calmodulin gene expression during embryogenesis of the sea urchin strongylocentrotus purpuratus was investigated. several identical bacteriophages containing a cdna insert encoding sea urchin calmodulin (cm-1) were identified by screening a lambda gt10 library of s. purpuratus gastrula-stage cdnas with a chicken calmodulin cdna sequence. a 1.2-kb cdna fragment from cm-1 was subcloned into puc-8 to give plasmid pcal-8. pcal-8 contains a single open reading frame encoding 79 amino acids, a termina ...	1986	3753945
studies on cardiac distribution and function of neuropeptide y.	high concentrations of a novel peptide, neuropeptide y, have been demonstrated in the guinea-pig and canine heart and in the latter, a particularly high concentration was found in the region of the coronary vasculature (126 +/- 31 pmol g-1). intra-arterial infusion of neuropeptide y for 30 s into the coronary artery of the intact, innervated dog heart resulted in a rapid and short-lasting reduction of blood flow from 38 +/- 4 to 31 +/- 3 ml min-1 (p less than 0.05) to resume control level, 39 +/ ...	1986	3754377
effect of alterations in lipid packing order by hydrophobic solutes on the association state of protein assemblies in model membranes.	the effects of two hydrophobic solutes which perturb lipid packing order, permethrin and allethrin, on the aggregated state of a lipid membrane-incorporated protein, bacteriorhodopsin (br), have been determined by resonance energy transfer measurements. as temperature is increased from well below the main gel-fluid phase transition temperature (tc) of the lipid, patches of aggregated br dissociate into monomers, a few degrees below the tc (m.p. heyn, a. blume, m. rehorek and n.a. dencher, bioche ...	1986	3754778
stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation.	stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ...	1986	3756629
breath hydrogen concentration and small intestinal malabsorption in calves.	breath hydrogen concentrations were measured to assess intestinal carbohydrate malabsorption in preruminating calves. oral administration of 1.25 g of lactulose (a nonabsorbable carbohydrate)/kg to calves produced breath hydrogen concentrations significantly (p less than 0.001) higher than values determined after calves were fed milk and before the treatment was given. this indicates that, in the calf, fermentation of nonabsorbed carbohydrates results in increased breath hydrogen values. to indu ...	1986	3767107
sensitive analytical method for serum primidone and its active metabolites for single-dose pharmacokinetic analysis in human subjects.		1986	3769107
participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and m. paratuberculosis in serum.	serum, which is low in freely-available iron, was bacteriostatic for m. avium and m. paratuberculosis irrespective of the ability of these organisms to produce the iron-chelators mycobactin and exochelin for iron acquisition. bacteriostasis was overcome when the serum was supplemented with exochelin or mycobactin, but only if the mycobacteria had previously adapted to growth in a low iron environment. the results imply that for studies on mycobacterial pathogenicity the test cultures should be g ...	1986	3788345
isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract.	a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ...	1986	3788570
bioluminescence in a strain of the human pathogenic bacterium vibrio vulnificus.	we report the existence of a bioluminescent strain of the human pathogen vibrio vulnificus. the isolate was obtained from blood following a fatal wound infection and thus represents the first description of an infection caused by a luminescent bacterium.	1986	3789716
rapid serological identification of vibrio vulnificus by anti-h coagglutination.	staphylococcus aureus cowan 1 cells were armed with anti-flagellar (anti-h) antibody produced in rabbits immunized with flagellar core protein prepared from vibrio vulnificus. this reagent was assessed by coagglutination for its capacity to agglutinate and identify v. vulnificus. a species-specific h antigen is expressed in the core proteins of the polar flagella of v. vulnificus. of 435 v. vulnificus isolates identified bacteriologically, 432 (99.3%) were agglutinated in the slide test within 2 ...	1986	3789720
comparison of histones in normal and xeroderma pigmentosum lymphoblastoid cells.	histones from normal human and xeroderma pigmentosum, complementation group a (xpa), lymphoblastoid cells were compared both quantitatively, qualitatively and for binding affinity for dna. electrophoretic examination of the histones showed that all five major histone species were present in both cell groups and that there were no quantitative differences between normal and xpa histones. binding affinity to [3h] mammalian dna of the histones was determined. no significant differences were observe ...	1986	3791423
surgical management of peritoneal dialysis catheters in children: five-year experience with 1,800 patient-month follow-up.	currently at our institution more than 90% of the children with end-stage renal disease are managed with continuous ambulatory peritoneal dialysis (capd) in preference to hemodialysis until a successful transplant is accomplished. recent refinements in capd catheters and dialysis techniques have greatly added to the many medical, psychological, and economic advantages of capd compared with chronic hemodialysis. ninety-three patients less than 21 years of age underwent insertion of 167 peritoneal ...	1986	3794985
[a case of vibrio vulnificus infection].		1986	3795561
[changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct].	competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease.	1986	3801148
susceptibility to varicella-zoster virus among adults at high risk for exposure.	the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ...	1986	3019915
susceptibility to varicella-zoster virus among adults at high risk for exposure.	the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ...	1986	3019915
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.	activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...	1986	3020234
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.	activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...	1986	3020234
mapping of the class ii region of the human major histocompatibility complex by pulsed-field gel electrophoresis.	the class ii region of the human major histocompatibility complex (mhc) encodes a polymorphic set of cell surface glycoproteins involved in the regulation of the immune response. each glycoprotein is a heterodimer composed of a alpha-chain of relative molecular mass (mr) 34,000 (34 k) and a beta-chain of mr = 28k. the products of the class ii region have been characterized by the mixed lymphocyte reaction, serology, primed lymphocyte typing and dna cloning. dr, dq and dp, three subregions contai ...	1986	3020430
mapping of the class ii region of the human major histocompatibility complex by pulsed-field gel electrophoresis.	the class ii region of the human major histocompatibility complex (mhc) encodes a polymorphic set of cell surface glycoproteins involved in the regulation of the immune response. each glycoprotein is a heterodimer composed of a alpha-chain of relative molecular mass (mr) 34,000 (34 k) and a beta-chain of mr = 28k. the products of the class ii region have been characterized by the mixed lymphocyte reaction, serology, primed lymphocyte typing and dna cloning. dr, dq and dp, three subregions contai ...	1986	3020430
production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury.	the migration and proliferation of smooth muscle cells (smcs) within the intima of arteries following mechanical injury is thought to be initiated by vessel wall injury and release of growth factors, in particular the platelet-derived growth factor (pdgf). however, the mechanism by which smc proliferation is regulated after platelet interaction with the vessel wall has ceased is unknown. here we show that smcs derived from the intima of injured rat arteries (intimal smcs) are phenotypically dist ...	1986	3020543
production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury.	the migration and proliferation of smooth muscle cells (smcs) within the intima of arteries following mechanical injury is thought to be initiated by vessel wall injury and release of growth factors, in particular the platelet-derived growth factor (pdgf). however, the mechanism by which smc proliferation is regulated after platelet interaction with the vessel wall has ceased is unknown. here we show that smcs derived from the intima of injured rat arteries (intimal smcs) are phenotypically dist ...	1986	3020543
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
[structure and functions of cloned adenovirus dna].		1986	3020801
subtypes and functions of alpha-adrenoceptors.		1986	3020944
thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus.	the acyclovir resistant mutant of varicella-zoster virus acv-r (a 8) induced the same level of thymidine kinase activity in infected cells as the parent kawaguchi strain. however, it induced less deoxycytidine kinase activity and did not induce phosphorylating activity for the nucleotide analogue, 9-(2 hydroxy-ethoxymethyl)-guanine-(acyclovir). another acyclovir resistant mutant, acv-r (a 4), which is cross-resistant to phosphonoacetate and is thought to be a viral dna polymerase mutant, induced ...	1986	3022711
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.	although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...	1986	3022716
synthesis and quantitative structure-activity relationships of antiallergic 2-hydroxy-n-1h-tetrazol-5-ylbenzamides and n-(2-hydroxyphenyl)-1h-tetrazole-5-carboxamides.	the synthesis and antiallergic activity of a series of 2-hydroxy-n-1h-tetrazol-5-ylbenzamides and isomeric n-(2-hydroxyphenyl)-1h-tetrazole-5-carboxamides is described. a relationship between structure and intravenous antiallergic activity in the rat passive cutaneous anaphylaxis (pca) test has been established using a hansch/free-wilson model and used to direct studies toward potent derivatives. the contribution of physicochemical properties to activity is discussed. one member of this series, ...	1986	2870188
report of a wound infection caused by vibrio parahaemolyticus and vibrio vulnificus.	the present case describes a foot wound caused by a clam shell from which both vibrio parahaemolyticus and vibrio vulnificus were recovered. although extraintestinal infections associated with vibrio parahaemolyticus have been reported previously, the simultaneous isolation of two marine vibrios from our case suggests that these organisms may coexist in mixed infections from a common source.	1986	2875824
effects of somatostatin and sms 201-995 on carbohydrate metabolism in normal man.	growth hormone (gh) is important in diabetes in view of its anti-insulin actions and its relation to the long-term complications of the disease. the suppression of gh secretion in diabetics has theoretical and possible therapeutic interest. native somatostatin has multiple actions, including inhibition of the secretion of insulin, glucagon, thyroid-stimulating hormone (tsh), and various gut hormones. it also has inhibitory effects on gastrointestinal motility, exocrine secretion, nutrient absorp ...	1986	2876501
serotonin and 5-hydroxyindoleacetic acid in brains of suicide victims. comparison in chronic schizophrenic patients with suicide as cause of death.	serotonin (5-ht) and 5-hydroxyindoleacetic acid concentrations were determined in various brain areas of nonschizophrenic suicide victims, chronic schizophrenic patients with or without suicide as the cause of death, and normal control subjects without psychiatric or neurologic disorders. serotonin concentrations in the basal ganglia were significantly elevated in suicide victims and chronic schizophrenic patients, as were 5-hydroxyindoleacetic acid concentrations in the occipital cortex. these ...	1986	2423050
identification and characterization of the nmyc gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities.	increased n-myc (now designated nmyc in human gene nomenclature) gene expression has been detected at the transcriptional level in certain types of neoplasms. as yet, the n-myc gene product has not been identified. to detect and characterize the n-myc gene product, we have developed monoclonal antibodies against the putative n-myc gene product made in escherichia coli as a fusion protein. the antibodies that recognize the n-myc-specific regions were selected on the basis of their reactivities to ...	1986	2426708
[effects of enzymatic deglycosylation of human goiter thyroglobulin on its immunochemical properties].	thyroglobulin (tg), isolated from soluble iodoproteins by ammonium sulphate fractionation, was enzymatically deglycosylated in vitro and analyzed by polyacrylamide gel electrophoresis, double immunodiffusion and non-commercial ria. carbohydrate and iodine content was chemically determined. by paage deglycosylated tg (dtg) showed the appearance of a major band in the 12s region and three slower migrating bands corresponding to higher aggregates than 19s tg. in immunodiffusion by testing native an ...	1986	2430684
esculin hydrolysis by vibrio vulnificus.	a clinical isolate of vibrio vulnificus was found to hydrolyze esculin when tested on bile-esculin-azide agar during the initial characterization of the strain. reports in the literature of esculin hydrolysis by v. vulnificus are conflicting. we tested herein 52 strains of v. vulnificus from clinical and environmental sources for the ability to hydrolyze esculin. seventy-eight percent of the strains hydrolyzed esculin on bile-esculin-azide agar, whereas all strains of v. vulnificus tested were p ...	1986	3943291
comparison of particle-associated progesterone and oxytocin in the ovine corpus luteum.	the subcellular distribution of progesterone and oxytocin within the ovine corpus luteum was investigated using differential and density gradient centrifugation. progesterone and oxytocin were associated with particles which sedimented to a density of 1.049-1.054 g/ml and 1.054-1.061 g/ml respectively. particle-associated progesterone did not, however, display physical or biochemical characteristics consistent with its storage within secretory granules. when particle-associated progesterone was ...	1986	3944530
gastroenteritis in patients with stool isolates of vibrio vulnificus.	vibrio vulnificus, a marine vibrio associated with severe extraintestinal infections, has not been previously implicated as a cause of infectious diarrhea. three patients were identified with diarrheal illness from whom this organism was the sole pathogen recovered from cultured stool specimens. all three had eaten raw oysters within one week of becoming ill. these patients were all taking medication that reduces gastric acidity, two were heavy drinkers of alcohol, and one had unrecognized colon ...	1986	3946453
detection of anti-vibrio vulnificus cytolysin antibodies in sera from mice and a human surviving v. vulnificus disease.	an enzyme-linked immunosorbent assay and a cytolysin neutralization assay were used detect anti-vibrio vulnificus cytolysin antibodies in sera from mice and a human that survived v. vulnificus disease. the detection of antibodies against the cytolysin indicated that the cytolysin is produced in vivo, and this observation is consistent with the hypothesis that the cytolysin is involved in the pathogenesis of v. vulnificus disease.	1986	3949387
inland presentation of vibrio vulnificus primary septicemia and necrotizing fasciitis.		1986	3953073
anal submucosal injection: a new route for drug administration in pelvic malignancies. part i. experimental study of misonidazole distribution in serum and tissues, with special reference to urinary bladder. preliminary report.	the anal and oral administration routes were compared in 30 rats to study the distribution of misonidazole, a radiation sensitizer, in the serum and tissues with special reference to the urinary bladder. 14c-labelled misonidazole was administered in a dose of 0.2 ml water/100 gm body weight containing 1 mu ci misonidazole. the dose was given orally by stomach tube in 15 rats, and was injected in the submucosa of the anal canal in another 15. animals were then killed after 15, 30, 60, or 120 minu ...	1986	3957602
emergence of bactericidal and opsonizing antibody to vibrio vulnificus following bacterial infection.	virulent isolates of vibrio vulnificus resist the bactericidal and opsonizing effects of normal human serum, in contrast to environmental isolates, which are highly serum susceptible. immune responses to bacteremic v. vulnificus infections in human subjects have not been characterized. serum from a patient who survived sepsis caused by v. vulnificus had substantial bactericidal and opsonizing immunoglobulin g (igg) for his own bloodstream isolate. killing was mediated by the classical complement ...	1986	3958138
degenerative lesions of a coronary chemoreceptor and nearby neural elements in the hearts of victims of sudden death.	a coronary chemoreceptor and its neighboring nerves and ganglia were studied by serial sections of tissue taken from the hearts of sixteen victims of sudden unexpected death. either focal or extensive inflammatory destruction was present in the chemoreceptors of 13 of the 16 hearts, and similar abnormalities of local nerves and ganglia were present in all 16 hearts. since an identical coronary chemoreceptor in the dog has previously been shown to be the site of origin of a powerful reflex with m ...	1986	3837961
evaluation of the hereditary syrian hamster cardiomyopathy by 31p nuclear magnetic resonance spectroscopy: improvement after acute verapamil therapy.	the relation between metabolic and functional derangement in various cardiomyopathies has not been well characterized. this information was specifically sought in a spontaneous cardiomyopathic model. metabolic and hemodynamic parameters were obtained in glucose-perfused beating hearts of 180-200-day-old cardiomyopathic syrian hamsters and age-matched healthy animals. this period in the cardiomyopathic hamster lifetime is intermediary between the necrotic phase and the appearance of heart failure ...	1986	3815756
processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes.	in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ...	1986	3818579
similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling.	we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ...	1986	3820095
[mast cell-affinity lymphocytes as indicator cells of the forms of hemoblastoses].	the formation of mastolymphocyte rosettes was used to determine that in chronic lymphocytic leukemia and bovine leukemia, unlike healthy people and animals as well as patients with solid tumours of different localization and hodgkin's disease, a large amount of cells appear among peripheral blood lymphocytes having membrane affinity for mast cells. this may be used as an additional criterion in laboratory diagnosis of these diseases.	1986	3485042
experience with isoxicam and catabolin.	1 synovial cells produce a protein factor, catabolin, that induces chondrocytes to resorb their own matrix with the release of glycosaminoglycan (gag). 2 studies were performed to determine the effect of isoxicam, a new nsaid, on gag production in a catabolin system utilizing tissue culture of bovine nasal septum in a medium containing pooled synovial tissue from young pigs. 3 using fresh medium containing no synovial tissue, the release of gag by inherent cartilage breakdown was 49.7% after 8 d ...	1986	3497659
[human gingival pigmentation due to electrochemical degradation. immunological study of biopsies using monoclonal antibodies].		1986	3464603
[a case of sepsis, shock, disseminated intravascular coagulation, and necrotizing fasciitis caused by vibrio vulnificus treated with early debridement].		1986	3559356
ranking possible carcinogenic hazards.	this review discusses reasons why animal cancer tests cannot be used to predict absolute human risks. such tests, however, may be used to indicate that some chemicals might be of greater concern than others. possible hazards to humans from a variety of rodent carcinogens are ranked by an index that relates the potency of each carcinogen in rodents to the exposure in humans. this ranking suggests that carcinogenic hazards from current levels of pesticide residues or water pollution are likely to ...	1987	3563506
[study on prostaglandin e involved in tooth movement--immunohistochemical observation].		1987	3475379
comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization.	the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ...	1987	3478194
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
tissue-specific and developmental expression of human transthyretin gene in transgenic mice.	to analyze the regulation of transthyretin gene expression we have produced transgenic mice by microinjecting cloned human transthyretin genes into fertilized eggs of c57bl/6 mice. the 7.6-kilobase (kb) human transthyretin gene containing about 500 base pairs (bp) in the upstream region was used for microinjection. seven out of nine transgenic mice had detectable amounts of human transthyretin in serum when analyzed by enzyme-linked immunosorbent assay. transthyretin mrna was detected in liver a ...	1987	3502973
a quantitative morphological examination of bovine vulval skin glands.	in adult cows the volumes of sweat and sebaceous glands per unit skin surface area (ssa) in the vulva were greater (p less than 0.01) than in the neck, lip or eyelids. within this perineal region skin gland volumes were greater dorsally than ventrally (p less than 0.01). adult cows had much greater (p less than 0.01) volumes of perineal skin glands per unit ssa than 8 weeks or 6 weeks old heifer calves or steers. there was little or no difference between animal categories in neck skin gland dime ...	1987	3503046
isolation and properties of a novel igg-binding protein from streptococci of serological group u.	a nonimmune binding of immunoglobulin (ig) g has been detected in streptococci of group u. the group u fc-binding site differed from the five previously known types of staphylococcal and streptococcal fc-binding sites by its strong affinity for murine igg, with dissociation constants in nanomolar range for rat and mouse igg, as well as for mouse igg subclasses 1, 2a, 2b and 3. it also differed from other binding sites by the high sensitivity towards trypsin. the fc-binding protein could be solub ...	1987	3821654
reaction to injectable collagen: results in animal models and clinical use.	since its commercial release, zyderm collagen implant has been used to treat more than 200,000 subjects in the united states for soft-tissue contour defects and more than 250,000 patients internationally (including the united states). approximately 3 percent of subjects' skin tested with zyderm collagen experience localized hypersensitivity reactions to collagen, whereas approximately 1 percent of treated patients demonstrate symptoms of hypersensitivity at treatment sites. of the latter treatme ...	1987	3823250
identification of environmental vibrio vulnificus isolates with a dna probe for the cytotoxin-hemolysin gene.	we screened 44 lactose-positive vibrio strains isolated from the marine environment for homology with a 3.2-kilobase dna fragment encoding the vibrio vulnificus cytotoxin-hemolysin gene. all 29 marine isolates identified as v. vulnificus on the basis of numerical taxonomy and dna-dna hybridization studies hybridized with the cytotoxin gene probe, as did all v. vulnificus reference strains. homologous gene sequences were identified in no other lactose-positive marine vibrio isolates nor in 10 oth ...	1987	3827248
clinical and ecological characteristics of vibrio vulnificus in the northeastern united states.	multiple seawater sites in the northeastern united states, particularly long island sound, and shellfish from long island sound were sampled from april to november for 3 successive yr, 1983-1985. hospitals in coastal and metropolitan areas of connecticut were surveyed for the same 3-yr period, vibrio vulnificus can be found in these waters during the summer months. the appearance of these virulent bacteria in both seawater and shellfish are a function of the water temperature; no v. vulnificus c ...	1987	3816129
vibrio vulnificus infection after raw oyster ingestion in a patient with liver disease and acquired immune deficiency syndrome-related complex.	sepsis, peritonitis, and gastroenteritis developed in a 45-yr-old homosexual man 1 day after ingestion of raw oysters. the patient had chronic active hepatitis and cirrhosis with hepatitis b virus and delta-infection. he also had persistent generalized lymphadenopathy associated with htlv-iii antibody positivity. vibrio vulnificus was isolated from the patient's blood and peritoneal fluid as well as from the same batch of oysters at the restaurant where the patient had visited. to our knowledge, ...	1987	3817400
correlation between virulence and colony morphology in vibrio vulnificus.	of 38 isolates of vibrio vulnificus examined, all avirulent strains produced only translucent colonies. all virulent strains, with the exception of biogroup 2 (eel pathogens), exhibited both opaque and translucent colonies. isogenic morphotypes were examined for a variety of phenotypic and virulence traits. only the ability to utilize transferrin-bound iron and the presence of a surface polysaccharide were found to correlate with colony opacity and virulence.	1987	2432016
the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr.	a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo.	1987	2435577
evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the oam.	an antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ...	1987	2446533
influence of neuropeptides on airway smooth muscle.	the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ...	1987	2446543
1,4-dithiothreitol-induced changes in histamine h1-agonist efficacy and affinity in the longitudinal smooth muscle of guinea-pig ileum.	the effect of 1,4-dithiothreitol (dtt) on histamine h1-receptor agonist affinity and efficacy has been investigated in longitudinal muscle strips of guinea-pig ileum. exposure of ileal smooth muscle to dtt significantly increased the maximal responses to the partial agonists skf71473 and de-2pea, indicative of an increase in agonist efficacy. this effect was paralleled by a small decrease in ec50 values. in contrast, dtt produced a parallel displacement of the concentration-response curve to the ...	1987	2880626
glutathione metabolizing enzyme activities in human thyroid.	glutathione peroxidases, glutathione transferase, glutathione reductase and gamma-glutamyl transpeptidase activities were analyzed in human thyroid tissues obtained from 17 patients undergoing resectional surgery because of a malignancy. it was deduced, from measurements of glutathione peroxidase activity with both h202 and cumene hydroperoxide, that thyroid contains only the selenium enzyme. the absence of selenium independent glutathione peroxidase activity in thyroid was confirmed with gel fi ...	1987	2883074
absence of age-related changes in the binding of the beta adrenergic antagonist 125i-iodohydroxybenzylpindolol in rat heart.	the effect of age on the density and the affinity of beta adrenergic receptors was determined in the hearts of fischer 344 rats at three ages, 6, 12, and 24 months old. the binding of the beta adrenergic antagonist 125i-iodohydroxybenzylpindolol (ihyp), was used to quantitate and characterize cardiac beta adrenergic receptors. the maximal number of binding sites (bmax = f moles/mg of protein) were 26.3 +/- 2.5, 25.4 +/- 0.99, and 24.5 +/- 2.4 and the dissociation constants (kd = nm) were 0.166 + ...	1987	2888126
chlamydia trachomatis in antenatal clinics.		1987	2889053
[h2-antihistaminics. 35. synthesis and h2-antagonistic action of imidazolylmethylthioalkyl-substituted 1,2,4-triazoles].		1987	2889442
a unique t-cell line derived from an htlv-1-negative adult t-cell leukemia patient.	a unique t-cell line, designated atl-5t, was established from lymphoma cells in pericardial effusion of an adult t-cell leukemia (atl) patient not carrying htlv-1 provirus. the cell line is okt4 and/or leu3a+ and okt8 and/or leu2a+, but interleukin 2 receptor (il2r)- and htlv-1 provirus genome negative, and has cytogenetically abnormal karyotypes. the cell line contains rearranged t-cell receptor beta-chain gene, which was identical in rearrangement pattern to the t-cell receptor beta-chain gene ...	1987	2890613
[mechanisms of hypergastrinemia after vagotomy].		1987	2824916
[mechanisms of hypergastrinemia after vagotomy].		1987	2824916
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
overlapping distributions of receptors for atrial natriuretic peptide and angiotensin ii visualized by in vitro autoradiography: morphological basis of physiological antagonism.	atrial natriuretic peptides exert actions on many key organs involved in blood pressure and water and electrolyte balance. many of these actions result in a physiological antagonism of angiotensin. to investigate the morphological basis of this interaction, we have mapped the distribution of receptors for atrial natriuretic peptide and angiotensin ii in a number of target organs, using 125i-labelled rat atrial natriuretic peptide (99-126) and 125i-labelled [sar1,ile8]angiotensin ii. in the kidne ...	1987	2825937
cytomegalovirus pneumonia.	cytomegalovirus (cmv) is a common virus that asymptomatically infects the majority of the population by early adulthood. after initial infection, the virus remains in a latent state in the host and can be reactivated under a variety of conditions. in contrast to the normal host, both primary and reactivated symptomatic cmv infections are common in certain immunocompromised patients including organ transplant recipients and patients with the acquired immunodeficiency syndrome (aids). one of the m ...	1987	2827281
the chicken carbonic anhydrase ii gene: evidence for a recent shift in intron position.	the complete nucleotide sequence of the coding region of the chicken carbonic anhydrase ii (ca ii) gene has been determined from clones isolated from a chicken genomic library. the sequence of a nearly full length chicken ca ii cdna clone has also been obtained. the gene is approximately 17 kilobase pairs (kb) in size and codes for a protein that is comprised of 259 amino acid residues. the 5' flanking region contains consensus sequences commonly associated with eucaryotic genes transcribed by r ...	1987	3029691
role of postnatal gonadal function in the determination of thyrotropin (tsh) releasing hormone-induced tsh response in adult male and female rats.	permanent effects of postnatal gonadal function on the hypothalamo-pituitary-thyroid axis were examined in male and female rats of the wistar-imamichi strain. animals were used at the age of about 10 weeks. neonatally castrated (nc) males showed a significantly higher plasma tsh response to trh (10 micrograms/kg bw, ip) than males castrated at 1, 2, 3, 4, or 6 weeks of age. the tsh response in intact males was similar to that seen in nc males. neonatal androgenization of nc males with 100 microg ...	1987	3032567
effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40.	to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ...	1987	3033894
maturation antigen of the mouse sperm flagellum: ii. origin from holocrine cells of the distal caput epididymis.	during epididymal transit, the mouse sperm flagellum acquires a surface glycoprotein (sma4) from epididymal fluid that functions as a sperm antiagglutinin. to determine the origin of this molecule, testes and epididymides of male mice were sectioned for light microscopy and stained with wheat germ agglutinin (wga)-peroxidase, a probe that has been used previously to examine the biology of sma4. wga reactivity was localized to the cytoplasm in a small population of cells in the distal caput epidi ...	1987	3034104
calmodulin-mediated cadmium inhibition of phosphodiesterase activity, in vitro.	ion-stripped bovine brain calmodulin (cam) binds 4 moles cd2+ as well as 4 moles ca2+ per mole protein, with similar affinity; in the presence of 1 mm mg2+ the molar binding ratio of cam for ca2+ decreased to 3, the apparent k0.5 for ca2+ nearly doubled, but the binding characteristics of cam for cd2+ were not changed. saturating concentrations ca2+ did not affect the molar binding ratio of cam for cd2+, but increased the apparent k0.5 for cd2+; vice versa, saturating concentrations cd2+ decreas ...	1987	3034196
radiation-induced dna damage and its repair.	application of modern methods of organic chemistry and recombinant dna technologies has provided new insights in the field of dna radiation damage and its repair. an overview of the chemical nature of the lesions inflicted on dna by ionizing radiation is presented. the structures of 29 different dna modified base or sugar residues are shown in comprehensive formation schemes. a fraction of radiation-induced modified bases is spontaneously released from the dna chain during irradiation. another p ...	1987	3034813
radiation-induced dna damage and its repair.	application of modern methods of organic chemistry and recombinant dna technologies has provided new insights in the field of dna radiation damage and its repair. an overview of the chemical nature of the lesions inflicted on dna by ionizing radiation is presented. the structures of 29 different dna modified base or sugar residues are shown in comprehensive formation schemes. a fraction of radiation-induced modified bases is spontaneously released from the dna chain during irradiation. another p ...	1987	3034813
identification of free radicals in myocardial ischemia/reperfusion by spin trapping with nitrone dmpo.	the spin trapping esr technique was applied to investigate oxygen-derived radicals in ischemic and post-ischemic rat hearts. using 5,5'-dimethyl-l-pyrroline-n-oxide, carbon-centered radicals were identified during ischemia and oxy-radical adducts (superoxide anion radical, o.-2 and hydroxyl radicals, .oh) in post-ischemic rat heart. the formation of these spin adducts was inhibited by superoxide dismutase, suggesting that superoxide plays a role in the adducts' formation. the results demonstrate ...	1987	3040465
examination of cardiac alpha-adrenoceptors from pharmacological responses and radioligand binding. comparison of rat and guinea pig tissues.	the object of this study was to determine suitable experimental conditions for the pharmacological evaluation of cardiac alpha-adrenoceptors. atrial and ventricular preparations of the guinea pig and rat were employed, and the alpha-adrenoceptor responsiveness was compared with the binding of the alpha-adrenoceptor radioligand [3h]prazosin in membranes prepared from these cardiac regions. the experimental variables examined were the pacing frequency, bath temperature, choice of agonist, and card ...	1987	3041119