Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| detection of genomic variation in providencia stuartii clinical isolates by analysis of dna restriction fragment length polymorphisms containing rrna cistrons. | chromosomal dna from 26 strains of providencia stuartii isolated mainly in hospitals in the united kingdom and reference strains of p. stuartii, p. rustigianii, and proteus vulgaris were digested with the restriction endonucleases ecori and hindiii. after electrophoresis in agarose gels, the fragments were subjected to southern blot hybridization analysis with a biotin-labeled cdna probe transcribed from a mixture of 16s and 23s rrna from p. stuartii nctc 11800t. the pattern of bands (the rdna f ... | 1988 | 2903175 |
| enoxacin: in-vitro and animal evaluation as a parenteral and oral agent against hospital bacterial isolates. | enoxacin was evaluated in in-vitro tests and in studies of effectiveness and blood concentrations in the mouse. enoxacin was active against both susceptible and multiresistant hospital isolates of enterobacteriaceae, pseudomonas aeruginosa, haemophilus influenzae, neisseria gonorrhoeae and staphylococci. less susceptible were streptococci and anaerobes. of nine quinolones tested, only norfloxacin was equivalent in vitro. the mbcs of enoxacin were one- to twofold greater than the mics, and enoxac ... | 1988 | 3129392 |
| comparative in vitro activity of the new oral cephalosporin cefixime. | cefixime was 8 to 10 times more active than cefaclor and augmentin against isolates of escherichia coli, klebsiella pneumoniae and salmonella typhi, mic90 values ranging from 0.06 to 0.25 micrograms/ml. however, none of these three drugs was particularly active against isolates of more resistant gram-negative bacilli such as enterobacter, serratia, citrobacter, acinetobacter, providencia and achromobacter spp. the lowest mic values for gram-negative bacilli were seen with ciprofloxacin, except f ... | 1988 | 3137053 |
| mechanism of inhibition of chromosomal beta-lactamases by third-generation cephalosporins. | the kinetic interactions of the beta-lactamase from enterobacter cloacae 908 r with ceftriaxone, cefotaxime, and ceftazidime have been examined in detail. with all of these cephalosporins, there is an initial rapid reaction involving opening of the beta-lactam that then decreases to a slower steady-state rate (kss) of beta-lactam hydrolysis (at 37 degrees c: ceftriaxone, kss = 0.044 s-1; cefotaxime, kss = 0.033 s-1; ceftazidime, kss = 0.011 s-1). more than stoichiometric quantities of beta-lacta ... | 1988 | 3142012 |
| further differentiation of enterobacteriaceae by means of siderophore-pattern analysis. | by means of a combination of 5 siderophore bioassays using several indicator strains, genera, species and subspecies of enterobacteriaceae can be further differentiated. enterobactin, aerobactin and other siderophores produced can be detected. each strain shows specific pattern which we called siderophore-pattern. it is easy to separate morganella, proteus, providencia, yersinia strains from the genera salmonella, shigella, escherichia coli, enterobacter, citrobacter, klebsiella, serratia. enter ... | 1988 | 2970195 |
| [nosocomial infections of the urinary tract in urology patients]. | researches effected in different nations have revealed that 5-12% of all hospitalized patients was affected with a nosocomial infection and that almost 1/3 of these were urinary tract infections. the hospitalized urological patients have a considerable proneness to contract a nosocomial urinary tract infection, because they are subjected to transurethral or suprapubic bladder catheterisms, to transurethral surgery and to the endoscopies, that are promoting maneuvers the urinary tract infections. ... | 1988 | 3340313 |
| numerical analysis of electrophoretic protein patterns of providencia alcalifaciens strains from human faeces and veterinary specimens. | twenty-five strains of providencia alcalifaciens from various countries have been characterized by one-dimensional sds-page of cellular proteins. they comprised 15 from human faeces, one from duck faeces, one from a guinea-pig eye and eight from unknown sources. also included, for reference purposes, were the type strains of three other providencia species. the protein patterns, which contained 45-50 discrete bands, were highly reproducible and were used as the basis for two numerical analyses. ... | 1988 | 3350783 |
| a survey of clinical experience with ciprofloxacin, a new quinolone antimicrobial. | ciprofloxacin is a new quinolone antimicrobial agent with activity against a broad spectrum of gram-negative and gram-positive organisms, including pseudomonas aeruginosa and methicillin-resistant strains of staphylococci. the efficacy and safety results of 80 clinical studies of the oral form of ciprofloxacin are reported. drug safety was assessed in 2236 courses in 2203 adult patients treated primarily in the united states. data from 1676 courses were suitable for analysis of drug efficacy. th ... | 1988 | 3360968 |
| new plate medium for screening and presumptive identification of gram-negative urinary tract pathogens. | a new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is described. the medium combines adonitol fermentation, phenylalanine deaminase, and beta-glucuronidase tests and allows the indole and cytochrome oxidase tests to be performed directly from the plates. high-level agreement with individual conventional tests was recorded in comparative studies with 504 cultures of gram-negative rods. there was 100% agreement, except for the providencia spp. i ... | 1988 | 3366875 |
| bacteremia in a veteran's administration extended care facility. | 1988 | 3380833 | |
| clinical correlates of bacteremia in a veterans administration extended care facility. | little is known about bacteremia in long-term care facilities. we have conducted a retrospective study during a 12-month period analyzing the clinical correlates of bacteremia in 533 chronically institutionalized, predominantly male patients, with an average age of 69 years. thirty-four men had forty-two bacteremic illnesses during this period. the incidence rate was 0.30 episodes per 1000 patient care days, and the mortality rate was 21%. the urinary tract was the most frequently identified tis ... | 1988 | 3042843 |
| [in vitro bactericidal effect of cefotetan-aminoside combinations]. | bactericidal activity of cefotetan-gentamicin combinations was studied on 6 bacterial strains: s. aureus, k. pneumoniae, e. cloacae, s. marcescens, p. vulgaris, p. stuarti. time kille curves technic was performed with final concentrations of cefotetan: 4 at 32 mg/l, and of gentamicin: 0.25; 0.5; 1; 2; 8 mg/l and with an 10(6) cfu/ml inoculum. cefotetan at 4 mg/l was not able to obtain a 0.01% (percentage of survivors) bactericidal activity before 24 h. the combination cefotetan-gentamicin (0.25 ... | 1988 | 3043329 |
| [in vitro antibacterial activity of apalcillin on aerobic bacteria and the regression curve]. | minimal inhibitory concentrations (mics) of apalcillin (apl) were evaluated by agar dilution on 1,201 bacterial strains isolated simultaneously in four university hospitals; agar diffusion tests (disks apl: 75 micrograms) were performed on these strains to establish relationship between mic and zone diameters. for enterobacteriaceae naturally non beta-lactamase-producing (e. coli and p. mirabilis), mode mic was 0.5 microgram/ml; some acquired penicillinase-producing strains were only inhibited b ... | 1988 | 3054743 |
| [evolution of bacterial resistance to five aminoglycosides. a study of 3,354 strains isolated in a hospital milieu]. | the authors studied the susceptibility to 5 aminoglycosides (amikacin, dibekacin, gentamicin, netilmicin and tobramycin) of 3,354 strains isolated at the centre hospitalier sud in bordeaux during 1987. the results are compared to those obtained in 1984 on 2,818 strains. amikacin remains the most active aminoside against the enterobacteriaceae and acinetobacter; against pseudomonas, tobramycin has become the best one at that time, as well as netilmicin against staphylococcus aureus. evolution: no ... | 1988 | 3054744 |
| inducible beta-lactamases: clinical and epidemiologic implications for use of newer cephalosporins. | the emergence of resistance to multiple beta-lactam antibiotics is a major problem in patients infected with organisms that characteristically produce inducible beta-lactamases--e.g., species of pseudomonas, enterobacter, serratia, citrobacter, indole-positive proteus, and providencia. resistance has emerged in 14%-56% of patients infected with these organisms and treated with one of the newer cephalosporins. the emergence of resistance has been associated with clinical failure or relapse in 25% ... | 1988 | 3055176 |
| in vitro activity of lomefloxacin (sc-47111; ny-198), a difluoroquinolone 3-carboxylic acid, compared with those of other quinolones. | lomefloxacin (sc-47111; ny-198) is a new difluoroquinolone agent. it inhibited 90% of escherichia coli, klebsiella spp., enterobacter spp., citrobacter spp., proteus mirabilis, morganella morganii, proteus vulgaris, serratia marcescens, salmonella spp., shigella spp., aeromonas spp., yersinia spp., haemophilus influenzae, and neisseria gonorrhoeae at less than or equal to 2 micrograms/ml. lomefloxacin inhibited 90% of pseudomonas aeruginosa at 4 micrograms/ml. lomefloxacin was equal in activity ... | 1988 | 3164987 |
| in-vitro and in-vivo activities of t-3262, a new pyridone carboxylic acid. | t-3262[p-toluenesulfonic acid salt of dl-7-(3-amino-1-pyrrolidinyl)-1-(2,4-difluorophenyl)-6-fluoro-1, 4-dihydro-4-oxo-1, 8-naphthyridine-3-carboxylic acid monohydrate] is a new pyridone carboxylic acid with a broad spectrum of antibacterial activity against gram-positive and gram-negative bacteria. the activity of t-3262 against most enterobacteriaceae was comparable with that of ciprofloxacin except proteus spp. and providencia rettgeri and exceeded that of ofloxacin and norfloxacin. the activ ... | 1988 | 3182417 |
| [evaluation on the capability of indole pyruvic acid production by members of proteeae on different brands of sulfide-indole-motility]. | members of proteeae were vertically inoculated into different brands of sulfide-indole-motility (sim) medium for evaluating the capability of ipa production. a total of 328 clinical strains of tribe proteeae was used as tested organisms, including 186 proteus mirabilis strains, 62 morganella morganii strains, 31 proteus vulgaris strains, 27 providencia rettgeri strains, 14 providencia stuartii and 8 providencia alcalifaciens strains. seven different brands of sim medium used in this study are ky ... | 1988 | 3197461 |
| in vitro activities of a-56619 (difloxacin) and a-56620, two aryl fluoroquinolones. | the in vitro activities of a56619 (difloxacin) and a-56620, two newer quinolones, have been compared with the activities of ciprofloxacin, ofloxacin, ceftazidime and netilmicin. a total of 782 clinical, bacterial isolates were employed. minimal inhibitory concentrations (mics) were determined under standard conditions with all isolates and, for 100 isolates against difloxacin and a-56620, with variation of agar ph and bacterial inoculum size. on a weight-for-weight basis, ciprofloxacin and a-566 ... | 1988 | 3208547 |
| [antibacterial activities of monobactams against fresh clinical isolates]. | antibacterial activities of monobactam antibiotics (carumonam (crmn) and aztreonam (azt] against gram-negative bacilli isolated from inpatients in the latter half of 1987 were investigated using penicillin (pc: piperacillin (pipc], cephems (ceps: ceftazidime (caz), cefotaxime (ctx), latamoxef (lmox), cefsulodin (cfs], carbapenem (imipenem (ipm] and pyridonecarboxylic acids (norfloxacin (nflx) and ofloxacin (oflx] as reference antibiotics. a total of 400 strains of 13 species, i.e. escherichia co ... | 1988 | 3210297 |
| [antibacterial activity of gentamicin against clinical isolates in pediatrics]. | antibacterial activity of gentamicin (gm), along with activities of other aminoglycosides and beta-lactams, was studied against clinical isolates collected from pediatric patients during a period of may 1986-april 1987. 1. gm-resistance was noted in 22% of staphylococcus aureus, 6% of proteus vulgaris, 8% of morganella morganii, 40% of providencia spp., 6% of enterobacter spp., 14% of serratia marcescens, and 14% of pseudomonas aeruginosa isolates. no gm-resistance was observed with isolates of ... | 1988 | 3216475 |
| in vitro activity of cefixime, a new oral cephalosporin. | the in vitro activities of cefixime and seven comparative oral antimicrobials were studied. mic90s of cefixime were 0.015-1 micrograms/ml for haemophilus influenzae, branhamella catarrhalis, escherichia coli, klebsiella, citrobacter diversus, proteus, providencia, aeromonas hydrophila and streptococcus. mic90s for other enterobacteriaceae were greater than or equal to 2 micrograms/ml; 44% of those strains were inhibited by less than or equal 1 microgram/ml. staphylococcus, psuedomonas aeruginosa ... | 1988 | 3219746 |
| in vitro activity of pefloxacin compared with five other quinolones on nalidixic acid-resistant proteae species. | the in vitro activity of pefloxacin, a new fluoroquinolone, was compared with that of 5 other quinolone compounds (nalidixic and pipemidic acids, norfloxacin, ciprofloxacin, and ofloxacin) against 416 strains of proteae spp. isolated from urine specimens of hospitalized patients with acute urinary tract infections (uti). ciprofloxacin was the most active agent. norfloxacin, ofloxacin, and pefloxacin were similarly active against proteus strains (mic90 = 0.39 microgram/ml). against providencia sp ... | 1988 | 3224396 |
| effect of pefloxacin on adherence of proteus-providencia spp to squamous epithelial cells. | pefloxacin, a new piperazinyl substituted quinolone derivative, reduced at sub-mics (minimal subinhibitory concentrations: four times lower than minimal inhibitory concentrations) the in vitro adherence of proteus-providencia spp. to squamous epithelial cells obtained from the urinary tract. the decrease of adherence was about 30% and 50% after 1 h and, respectively, 16-18 h of incubation with pefloxacin. this finding suggests that pefloxacin may play a role in determining the in vivo adhesion o ... | 1988 | 3224397 |
| isolation of multiple antibiotic resistant enterobacteriaceae from river water. | water samples from the monocacy river in frederick county, maryland, yielded twenty-four isolates which were resistant to tetracycline (ter, 25 micrograms/ml). although these organisms were not initially cultured on a coliform-selective medium, twenty-two of the isolates were gram-negative and carriers of antibiotic resistance to five or more antibiotics; erythromycin, methicillin, novobiocin, penicillin and tetracycline. of the isolates 45% were biochemically identified as providencia stuartii; ... | 1988 | 3241574 |
| activity of cefixime (fk 027) for resistant gram-negative bacilli. | cefixime is an orally absorbable cephalosporin with an extended spectrum of in vitro activity for gram-negative bacteria especially members of the enterobacteriaceae. gram-negative rod isolates collected over a three month period were tested against cefixime. greater than 99% of escherichia coli isolates was susceptible to cefixime including those resistant to ampicillin. similarly isolates of klebsiella, proteus and providencia were also exquisitely susceptible to cefixime as were 91% of entero ... | 1988 | 3243091 |
| resistance of bacterial pathogens to four aminoglycosides and six other antibacterials and prevalence of aminoglycoside modifying enzymes, in 20 uk centres. | a total of 5116 non-fastidious bacterial strains isolated from either blood or urine culture were collected from 20 geographically distributed centres within the uk. upon receipt the strains were identified and the minimum inhibitory concentrations of ten antimicrobial agents in hospital usage were determined for each of the strains. all laboratories submitted a similar range of bacterial species and in species normally considered as sensitive the overall rates of resistance to the different ant ... | 1988 | 3243733 |
| an evaluation of allozyme amino acid substitutions for the study of molecular relationships in providencia strains. | through analysis of molecular relationships in terms of amino acid substitutions, intra- and interspecies differentiations in providencia alcalifaciens, p. stuartii and p. rustigianii were evaluated among the electrophoretic variants of three enzymes, l-malate dehydrogenase, acid phosphatase and esterase-beta a, chosen for their distinct pattern of polymorphism. for each enzyme, molecular relatedness among variants defined by two-dimensional electrophoretic profiles was examined through protein ... | 1988 | 3252906 |
| disk diffusion susceptibility testing for ly163892 (kt-3777), a new orally administered 1-carbacephem. | ly163892, a new orally administered 1-carbacephem, was found to have a spectrum of antimicrobial activity very similar to that of cefaclor. diffusion tests with 30-micrograms ly163892 disks produced acceptable interpretive error rates with greater than or equal to 22 mm as the susceptibility zone diameter. this was required to reduce potential false-susceptibility results, particularly among enterobacter spp. and providencia stuartii: both species included strains that produce beta-lactamases ca ... | 1988 | 3259247 |
| in vitro activity of an oral iminomethoxy aminothiazolyl cephalosporin, r-3746. | the in vitro activity of r-3746, an iminomethoxy aminothiazolyl cephalosporin with a ch2och3 moiety at position 3, was compared with those of other antibiotics. r-3746 inhibited the majority of hemolytic streptococci (groups a, b, c, f, and g) and streptococcus pneumoniae at less than 0.06 micrograms/ml, which was comparable to the activity of amoxicillin, 2- to 8-fold more active than cefixime, and 16- to 64-fold more active than cefaclor and cephalexin. ninety percent of beta-lactamase-produci ... | 1988 | 3260766 |
| the effects of clavulanic acid and sulbactam on beta-lactamase biosynthesis. | clavulanic acid and sulbactam were tested as inducers of beta-lactamase in 21 strains of gram-negative bacteria. beta-lactamase synthesis was inducible in all these strains, as demonstrated by the increased enzyme activities obtained when cells were grown in the presence of 10 mg/l of cefoxitin, the increase varying from 11- to 734-fold however, at the same concentration, neither inhibitor induced significant amounts of beta-lactamase, except in one strain of providencia rettgeri, where both wer ... | 1988 | 3263350 |
| in-vitro activity and beta-lactamase stability of ly163892. | ly163892 is a new orally absorbed carbacephem. it inhibited streptococcus pyogenes and str. pneumoniae at less than or equal to 1 mg/l, but was less active against group b streptococci and groups c, f, g and bovis streptococci with mics of 1 to 2 mg/l for most but as high as 8 mg/l for some isolates. mic90 of methicillin-susceptible staphylococcus aureus was 8 mg/l, but greater than 128 mg/l for methicillin-resistant staphylococci. ly163892 had activity similar to cefaclor and cephalexin with mi ... | 1988 | 3263352 |
| in vitro and in vivo antibacterial activities of me1207, a new oral cephalosporin. | me1207 (pivaloyloxymethyl ester of me1206) is a new oral cephalosporin. me1206 is (6r,7r)-7-[(z)-2-(2-aminothiazol-4-yl)-2-(methoxyimino)- acetamido]-3-[(z)-2-(4-methylthiazol-5-yl)-ethyl]-cephem-4-carboxy lic acid. the susceptibilities of about 1,600 clinical isolates to me1206 were determined by the agar dilution method. me1206 showed a broad spectrum of activity against gram-positive and gram-negative bacteria. me1206 was more active than cefaclor, t-2525, and cefixime against staphylococcus ... | 1988 | 3264132 |
| production and properties of haemolysins from clinical isolates of the proteeae. | a collection of 198 clinical isolates of strains belonging to the tribe proteeae was examined for haemolytic activity on blood agar and in brain heart infusion broth. the strains were of diverse bacteriocin and o-antigenic types and from a wide variety of sources. they included representatives of all species of morganella, proteus and providencia. approximately half of the m. morgani strains were haemolytic on blood agar. this activity was not associated with any particular bacteriocin type. the ... | 1988 | 3275780 |
| a survey of iga protease production among clinical isolates of proteeae. | a collection of 100 strains of proteeae, in which all species within the tribe were represented, was examined for iga protease production. the strains were isolated from various clinical specimens from sick and healthy persons in several countries. iga protease-producing strains were not found amongst species of providencia and morganella but were common in proteus spp. all the strains of p. mirabilis and p. penneri and many of the strains of p. vulgaris examined produced an edta-sensitive prote ... | 1988 | 3275781 |
| rapidec ur, a 2-h miniaturized system for pinpointing uropathogens. | a miniaturized 2-h system (rapidec ur; api system, la balme-les-grottes, montalieu-vercieu, france) that uses nine enzymatic tests for the diagnosis of most uropathogens was challenged with 330 strains. with few exceptions (citrobacter, enterobacter, and providencia spp., morganella morganii, and staphylococci other than staphylococcus saprophyticus), there were distinct patterns that allowed at least a preliminary species diagnosis. | 1988 | 3277991 |
| commercial identification systems often fail to identify providencia stuartii. | we tested 145 clinical isolates in an attempt to evaluate some of the most widely used commercial identification systems in europe in terms of their ability to identify providencia strains. two manual miniaturized systems (api 20e and enterotube ii) and three mechanized-automated systems (cobas-bact, sceptor system, and titertek-enterobac-rapid automated system) were evaluated. providencia alcalifaciens and providencia rettgeri strains were correctly identified by all systems in all cases, and i ... | 1988 | 3277997 |
| tigemonam, an oral monobactam. | tigemonam is an orally administered monobactam. at less than or equal to 1 microgram/ml it inhibited the majority of strains of escherichia coli, klebsiella spp., enterobacter aerogenes, citrobacter diversus, proteus spp., providencia spp., aeromonas hydrophila, salmonella spp., shigella spp., serratia marcescens, and yersinia enterocolitica. at less than or equal to 0.25 microgram/ml it inhibited haemophilus spp., neisseria spp., and branhamella catarrhalis. it did not inhibit pseudomonas spp. ... | 1988 | 3279906 |
| evaluation of the ids rapid ss/u system for rapid identification of urinary microorganisms. | the accuracy of a new rapid identification system for common urinary pathogens was compared with that of conventional methods and of miniaturized 18-24-hour identification panels. the rapid system, rapid ss/u (innovative diagnostic system inc., atlanta, ga) is a non-growth-dependent micro-method that identifies selected gram-negative bacilli, gram-positive cocci, and yeasts in two hours by detection of constitutive enzymes acting on chromogenic substrates. a total of 185 representative clinical ... | 1988 | 3285661 |
| comparative in vitro activity of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone against hospital strains of gram-negative and -positive bacteria. | the in vitro antibacterial activities of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone were compared against 330 gram-negative and gram-positive strains isolated from clinical specimens received at the king abdulaziz university hospital (kauh) in saudi arabia. the antibacterial susceptibility was determinated by stokes method and by the minimal inhibitory concentration (mic) using an agar dilution method. ceftazidime and ceftriaxone were the most active antibiotics, inhibiting ... | 1988 | 3293818 |
| [the rapid identification of e. coli by the detection of 3 enzymes]. | 232 isolates of gram-negative, oxidase-negative bacteria, isolated from various samples of different animal species, were tested with help of rapidec coli for the production of beta-glucuronidase, beta-galactosidase and indole. the test correctly identified 164 of 175 e. coli strains (sensitivity 93.7%) and correctly indicated that 57 of 57 isolates of the family enterobacteriaceae (7 citrobacter sp., 18 enterobacter sp., 16 klebsiella sp., 10 proteus sp., 2 providencia sp. and 4 salmonella sp.) ... | 1989 | 2497726 |
| providencia alcalifaciens and travellers' diarrhoea. | 1989 | 2504344 | |
| in vitro antibacterial properties of cefetamet and in vivo activity of its orally absorbable ester derivative, cefetamet pivoxil. | the in vitro activity of cefetamet, the microbiologically active metabolite of the orally administered prodrug cefetamet pivoxil, was compared with that of cephalexin, cefaclor, cefuroxime and amoxicillin. cefetamet was highly active against enterobacteriaceae, neisseria spp., vibrio spp., haemophilus influenzae and streptococci other than enterococci. cefetamet inhibited cefaclor-resistant species such as proteus vulgaris, providencia stuartii, providencia rettgeri and serratia marcescens. stap ... | 1989 | 2504597 |
| purple urine bags. | purple urine drainage bags were found in 7 of 71 chronically catheterized elderly women. the purple staining of the bags is due to a violet discoloration (indirubin) of the plastic of the catheter bag and fine blue crystals of indigo in the urine. the colors are formed from the substrate indoxyl sulfate (indican) and all 7 patients had bacteria in the urine that would produce blue colonies on agar enriched with the urine (filter sterilized) of the patients involved. organisms identified were pro ... | 1989 | 2504940 |
| dactimicin, a new aminoglycoside: in vitro activity, post-antibiotic effect and interaction with other antibiotics. | the in vitro activity of the new aminoglycoside dactimicin in comparison to amikacin was tested alone and in combination with piperacillin, mezlocillin and ceftazidime against freshly isolated clinical pathogens. dactimicin was more active than amikacin against enterobacter cloacae, providencia rettgeri and salmonella spp., and less active than amikacin against escherichia coli, pseudomonas aeruginosa and acinetobacter anitratus. using the checkerboard technique, the combination of either dactim ... | 1989 | 2506028 |
| [in vitro antibacterial activity of a new fluoroquinolone, fleroxacin as a function of the sensitivity or resistance to nalidixic acid and to pefloxacin]. | minimal inhibitory concentrations (mic) of fleroxacin (fle) were evaluated by agar dilution for 375 bacterial strains. for enterobacteriaceae, mic of fle were (microgram/ml): nalidixic acid (nal), susceptible (s), pefloxacin (pef), susceptible (s) strains: 0.032 to 0.5; nal, resistant (r), pef s: 0.25 to 1; nal r, pef r: 1 to 32; nal r, pef r strains were particularly observed among serratia marcescens and providencia stuartii, pseudomonas aeruginosa was less susceptible to fle (mic 1 to 4), as ... | 1989 | 2506511 |
| the postantibiotic effect of meropenem and imipenem on selected bacteria. | controlled experiments were conducted to determine the in-vitro postantibiotic effect (pae) of meropenem and imipenem on ten selected bacteria representative of medically important species: staphylococcus aureus (2). pseudomonas aeruginosa (4), escherichia coli (1), serratia marcescens (1), morganella morganii (1), and providencia stuartii (1). the pae was determined by comparing serial colony counts of cultures recovering from exposure to drug concentrations at 4 x mic for 1.5 h with the counts ... | 1989 | 2509416 |
| in-vitro activity of meropenem imipenem, the penem hre 664 and ceftazidine against clinical isolates from west germany. | the antibacterial activity in vitro of meropenem was compared with another carbapenem, imipenem, the penem hre 664, and ceftazidime. mics were not influenced by ph nor by inoculum size below 5 x 10(5) cfu/ml: higher inocula caused a modest increase in mic. cation supplementation of mueller-hinton broth was without effect. meropenem was more active than imipenem and the other comparative agents against the majority of gram-negative species (in particular proteus, providencia, morganella, haemophi ... | 1989 | 2509418 |
| modification of norfloxacin inhibition of dna gyrase induced by a 28 kdal dna binding protein. | we have previously studied a clinical isolate of providencia stuartii which showed high levels of resistance to 4-quinolones, aminoglycoside and beta-lactam antibiotics (landini et al., 1987). dna gyrase from this isolate was inhibited for 50% of activity at a concentration of 15 microm of norfloxacin, which is about 5-fold higher compared to the 50% inhibitory concentration for a standard dna gyrase. it has been described that 4-quinolone inhibition of dna gyrase is caused by their binding to d ... | 1989 | 2550742 |
| in vitro and in vivo efficacy of ytr-830h and piperacillin combinations versus beta-lactamase-producing bacteria. | ytr-830h, a beta-lactamase inhibitor, is a non-amino penicillanic sulfone. in vitro synergistic activity with piperacillin was determined for 226 beta-lactamase producing clinical cultures. combination of piperacillin: ytr in ratios of 2:1, 4:1, and 8:1 were highly effective vs escherichia coli, proteus, providencia, morganella, staphylococcus, and bacteroides. minimum inhibitory concentrations (mics) of piperacillin were reduced from the resistant to susceptible range. the higher ratios were le ... | 1989 | 2552030 |
| comparative in vitro activities of piperacillin-tazobactam and ticarcillin-clavulanate. | the in vitro activities of ticarcillin, piperacillin, clavulanic acid, tazobactam, ticarcillin-clavulanate, and piperacillin-tazobactam against 819 bacterial isolates were compared. the two beta-lactamase inhibitors, clavulanic acid and tazobactam, had little useful antibacterial activity but enhanced the activities of the penicillins against beta-lactamase-producing strains of haemophilus influenzae, branhamella catarrhalis, and methicillin-susceptible staphylococcus aureus; all strains were su ... | 1989 | 2552904 |
| studies to optimize the in vitro testing of piperacillin combined with tazobactam (ytr 830). | the combination of piperacillin and the beta-lactamase inhibitor tazobactam (formerly ytr 830) was studied to determine optimal disk concentrations and dilution testing conditions. in addition, the potency of the combination was compared to that of piperacillin alone. the spectrum of piperacillin was greatly expanded by the addition to tazobactam principally against beta-lactamase producing strains of haemophilus influenzae, escherichia coli, morganella morganii, proteus vulgaris, providencia st ... | 1989 | 2560422 |
| type 3 fimbriae among enterobacteria and the ability of spermidine to inhibit mr/k hemagglutination. | the distribution of the gene cluster encoding type 3 fimbriae among various isolates of the family enterobacteriaceae was investigated by using 112 clinical and nonclinical isolates. closely related dna sequences were detected in all klebsiella strains, in most enterobacter isolates, in a smaller number of escherichia coli and salmonella spp., and in a single isolate each of yersinia enterocolitica and serratia liquefaciens but not in isolates of morganella or providencia species or serratia mar ... | 1989 | 2562835 |
| usefulness of trehalose fermentation and l-glutamic acid decarboxylation for identification of biochemically aberrant providencia stuartii strains. | a total of 849 providencia isolates were collected during a 4-year period when an increased incidence of nosocomial providencia stuartii infection was noted in urologic wards. of these isolates, 630 were identified as p. stuartii, 206 were identified as providencia rettgeri, and 1 was identified as providencia alcalifaciens. twelve inositol-positive isolates from 10 patients (10 strains) resembled p. stuartii in fermenting trehalose but resembled p. rettgeri in fermenting d-arabitol or meso-eryt ... | 1989 | 2570791 |
| numerical analysis of electrophoretic protein patterns of providencia rettgeri strains from human faeces, urine and other specimens. | thirty-one strains of providencia rettgeri (mainly from humans) were characterized by one-dimensional sds-page of cellular proteins. the strains came from various countries and comprised 14 from urine, eight from faeces, two from bile (plus one from the liver of a sheep), two from sputum, one from an insect pupa and three the sources of which were unknown. also included, for reference purposes, were the type strains of the four other providencia species. the protein patterns, which contained 45- ... | 1989 | 2584173 |
| comparative in vitro activity and beta-lactamase stability of fk482, a new oral cephalosporin. | fk482 is an oral aminothiazolyl hydroxyimino cephalosporin with a c-3 vinyl group. its activity was compared with those of cephalexin, cefuroxime, cefixime, and amoxicillin-clavulanate. fk482 inhibited 90% of staphylococcus aureus isolates at 1 micrograms/ml and 90% of streptococcus pyogenes, streptococcus agalactiae, and streptococcus pneumoniae isolates at less than or equal to 0.012 micrograms/ml, superior to cephalexin and cefuroxime and similar to cefixime. it did not inhibit oxacillin-resi ... | 1989 | 2589845 |
| mechanisms of gentamicin resistance in gram-negative bacilli in riyadh, kingdom of saudi arabia. | a survey was undertaken of the prevalence and mechanisms of gentamicin resistance in gram-negative bacilli in riyadh. gentamicin resistance, as assessed by the stokes method, occurred in less than 2% of isolates of escherichia coli, about 10-25% of most other enterobacteria, about 40% of acinetobacter spp. and about 25% of pseudomonas aeruginosa. this finding of relatively low rates of gentamicin in enterobacteriaceae was surprising in view of the unregulated use of antibiotics until recent year ... | 1989 | 2599993 |
| [determination of the mic of cefotetan against freshly isolated gram-negative bacilli]. | antimicrobial activities (mics) of cefotetan (ctt) against 575 strains of 16 spp. of gram-negative bacilli isolated in 1988 were determined to investigate distribution of mics in comparison with those of cefmetazole (cmz), cefoxitin (cfx), latamoxef (lmox) and cefazolin (cez). the change in frequencies of incidence of cephem-resistant strains in the latter half of the 1980 was also investigated. distribution of mic of ctt varied with test strains. no or very few mics were at or higher than 12.5 ... | 1989 | 2621779 |
| [bacteriologic study of abscesses caused by bites of snakes of the genus bothrops]. | the bacterial flora of 99 cases of abscesses following bothrops snakebite were analysed. they corresponded to 61.1% of all snakebite abscesses observed in 1030 patients attending the hospital de doenças tropicais de goiánia in goiás, brazil, from january 1984 to april 1988. an exsudate sample of each abscess was examined by gram stain, culture and susceptibility tests. the gram negative bacillis, morganella morganii, escherichia coli and providencia sp were the most frequent bacterias isolated. ... | 1989 | 2640511 |
| rapid identification of salmonella from poultry meat products by using 'mucap test'. | a study was made in order to improve a new salmonella identification test (mucap test) in which umbelliferone is released, giving a blue fluorescent light under a wood lamp, after contact with salmonella colonies. the study concerned 354 colonies, previously isolated from 55 poultry meat samples. two enrichment media [tetrathionate bile broth (tbb) and rappaport vassiliadis (rv)] and two isolation media [brilliant green agar (bga) and desoxycholate agar (da)] were used, and the results of the te ... | 1989 | 2641489 |
| [the rapid identification of escherichia coli using bactident e. coli]. | 165 (93.7%) of 175 e. coli strains isolated from various materials of different animal species were correctly identified as e. coli with help of commercially available bactident e. coli (e. merck, d-6100 darmstadt). further 52 strains of the family of enterobacteriaceae (2x providencia sp., 2x salmonella sp., 7 citrobacter sp., 9 proteus sp., 15 klebsiella sp., 17 enterobacter sp.) were correctly not identified as e. coli by this test. bactident e. coli is a suitable test for rapid identificatio ... | 1989 | 2647078 |
| aztreonam in the treatment of urinary tract infections: a multicenter trial. | a multicenter trial involving several urologic units in italy provided pooled data on 1,427 patients with urinary tract infections to evaluate the efficacy and safety of aztreonam, a new monobactam antibiotic. microbiologic and clinical data were collected methodically in all cases. a majority of the patients (79.9%) were hospitalized during the study period, an associated pathology was noted in 29.1%, and 16.7% were receiving additional therapy. aztreonam was administered by different routes an ... | 1989 | 2659294 |
| comparison of the in vitro and in vivo antibacterial activities of cefepime (bmy-28142) with ceftazidime, cefuzonam, cefotaxime and cefmenoxime. | cefepime (bmy-28142), a new semisynthetic cephalosporin, was evaluated for in vitro and in vivo antibacterial activities in comparison with ceftazidime, cefuzonam, cefotaxime and cefmenoxime. cefepime showed a well-balanced, broad spectrum of activity against a number of clinical isolates collected in japan. the activity of cefepime against gram-positive bacteria was several times greater than that of ceftazidime, nearly comparable to cefotaxime and cefmenoxime, and slightly weaker than cefuzona ... | 1989 | 2663406 |
| interaction of the bisbiguanides chlorhexidine and alexidine with phospholipid vesicles: evidence for separate modes of action. | strains of providencia stuartii with demonstrated resistance towards chlorhexidine did not show such resistance towards either of the related biguanide antiseptics, alexidine or vantocil. alexidine promoted a significantly faster alteration in the permeability of escherichia coli cell membranes towards various metal cations than chlorhexidine. differential thermal analysis of various mixed lipid vesicles and pure phospholipid vesicles showed alexidine to share with vantocil the property of produ ... | 1989 | 2663802 |
| [evaluation of a new semi-automatic method of identifying enterobacteriaceae, m.i.s.-enterobacteriaceae]. | m.i.s.-enterobacteriaceae is a new kit for identifying enterobacteriaceae using a microplate consisting of 21 biochemical characters with automated reading and interpretation. the validity of this method was studied by the identification of 350 strains of enterobacteria belonging to 44 species and comparison with the classical method of identification in test-tubes. results showed a diagnosis accuracy of 96 p. cent at the species level and 97.7 p. cent at the genus level. diagnosis accuracy reac ... | 1989 | 2665580 |
| proteeae species bacteriuria accompanying proteeae species groin skin carriage in geriatric outpatients. | there have been numerous reports of proteeae species urinary tract infections among elderly individuals. to explore a possible urinary carrier source of proteeae species in this population, the frequency of aerobic gram-negative bacillus (agnb) bacteriuria at the greater than or equal to 10(2)/ml level was determined in 67 ambulatory elderly outpatients classified as to proteeae group (morganella, proteus, providencia) groin carriage by a set of two skin cultures obtained at least 1 week apart. ... | 1989 | 2674195 |
| in-vitro activity of meropenem against clinical isolates in a multicentre study in italy. | a multicentre in-vitro study was undertaken to evaluate the susceptibility of bacterial pathogens isolated in different italian hospitals to meropenem. a total of 1399 aerobic and 452 anaerobic strains was analysed. comparative agents were imipenem, cefotaxime, ceftazidime, ceftriaxone, piperacillin, ciprofloxacin, gentamicin, amikacin, plus vancomycin when appropriate. the mic ranges (mg/l) of meropenem were: 0.015-2 for klebsiella spp., proteus spp., morganella morganii and providencia spp.; l ... | 1989 | 2681128 |
| randomized, double-blind comparative study of intravenous ciprofloxacin versus ceftazidime in the treatment of serious infections. | we compared intravenously administered ciprofloxacin with ceftazidime in a randomized, double-blind study. patients received ciprofloxacin 200 mg intravenously every 12 hours or ceftazidime 2 g intravenously every eight hours, with placebo infusions to maintain blinding. therapy with metronidazole was added for suspected or documented intra-abdominal infection. thirty-two of the 57 ciprofloxacin-treated patients were evaluable for determination of efficacy and had 41 bacterial isolates from 34 s ... | 1989 | 2686416 |
| a comparative evaluation of oral ofloxacin versus intravenous cefotaxime therapy for serious skin and skin structure infections. | in a single-blind, placebo-controlled randomized trial, 100 successive patients were enrolled with serious skin and soft-tissue infections, whose illnesses had precipitated an initial hospital admission or an extension of inpatient care. there were 93 evaluable patients who received either ofloxacin, 400 mg orally every 12 hours plus an intravenously administered placebo every eight hours, or cefotaxime, 2.0 g intravenously every eight hours plus an orally administered placebo every 12 hours. th ... | 1989 | 2690621 |
| the role of proteae in diarrhea. | a survey was undertaken on the occurrence of protease in the human fecal flora and its coincidence with other well-documented enteropathogens such as campylobacter, salmonella, shigella, staphylococcus aureus, yersinia, protozoa and rotavirus. a total of 2000 fecal specimens was investigated, 1000 from patients suffering from diarrhea and 1000 from healthy persons which served as controls. proteus mirabilis was isolated more frequently from diarrhea cases than from healthy people. the difference ... | 1989 | 2692584 |
| aminoglycoside resistance patterns in clinical isolates of e. coli and klebsiella sp. from czechoslovakia and the united states. | resistance of gram-negative bacilli to aminoglycoside antibiotics differs by region and country. previous studies have demonstrated predominance of the nucleotidyltransferase anl(2") as the mechanism of enzymatic resistance to gentamicin in the united states and many european countries (federal republic of germany, switzerland, greece, turkey) whereas the acetylating enzymes aac(6') and aac(3) were the principal causes of resistance to aminoglycosides in japan and chile. in the present compariso ... | 1989 | 2696761 |
| in-vitro interactions of fce 22101 with aminoglycosides against gram-negative rods. | agar dilution mics of fce 22101 were measured for 894 consecutively-isolated gram-negative rods from clinical specimens (413 escherichia coli, 104 klebsiella spp., 54 enterobacter spp., 19 citrobacter spp., 131 proteus spp., 43 acinetobacter spp., 9 serratia spp., 3 providencia spp., 4 yersinia spp., 3 hafnia spp. and 111 pseudomonas spp.). excluding pseudomonas spp., 98% of these isolates were susceptible to 8 mg/l fce 22101. resistance (mic greater than 8 mg/l) varied from 11% in serratia spp. ... | 1989 | 2732132 |
| comparative in vitro susceptibilities of 504 bacteremic isolates to ticarcillin plus clavulanic acid and other antimicrobial agents. | a total of 504 clinical bacteremic isolates were tested for susceptibility to ticarcillin-clavulanic acid and 12 other antibiotics. ticarcillin-clavulanic acid showed superior antibacterial activity compared to penicillin, mezlocillin, piperacillin, ticarcillin, gentamicin, and amikacin against bacteremic isolates of methicillin-susceptible staphylococcus aureus and staphylococcus epidermidis. however, ticarcillin-clavulanic acid's activity was inferior to that of vancomycin against methicillin- ... | 1989 | 2752713 |
| antibiotic sensitivity of enterobacteriaceae by disc diffusion and rapid fermentation sensitivity test methods. | a total of 400 strains of gram negative bacilli were examined both by conventional disc diffusion (dd) and by rapid fermentation sensitivity test (rfst) methods. various strains which were examined included esch. coli kleb. aerogenes, kleb. pneumoniae, prot. vulgaris, prot. mirabilis, prot. morgani, prot. rittgeri and providencia. the antibiotics used were streptomycin, chloramphenicol, kanamycin, gentamycin and ampicillin. the correlation between the two methods was found to vary from 75 to 98 ... | 1989 | 2777349 |
| blockage of urinary catheters: role of microorganisms and constituents of the urine on formation of encrustations. | long-term indwelling urinary catheters may become blocked in some patients by formation of encrustations made up of aggregated struvite crystals while other patients rarely develop blocked catheters. we have designated these groups as "blockers", "intermediates" or "non-blockers". to further understand this phenomenon we followed 32 catheterized elderly women in a nursing home. catheters were changed six times at 2 week intervals. patients tended to remain as "blockers", "intermediates" or "non- ... | 1989 | 2778465 |
| induction potency of various beta-lactam derivatives in gram-negative rods. | the induction potency of various beta-lactams as well as that of 'nonspecific' inducers such as the media employed or body fluids were studied in gram-negative clinical isolates and in their resistant corresponding counterparts. in all wild-type isolates quite a few beta-lactams (mainly cefoxitin and imipenem) shared the ability to induce the chromosomal beta-lactamase, whereas all beta-lactams - including 6-aps and 7-cps clavulanic acid, and sulbactam (both beta-lactamase inhibitors), exhibited ... | 1989 | 2785904 |
| in-vitro activity of fce 22101 and synergy studies with other antimicrobial agents. | fce 22101 is a penem antibiotic with broad in-vitro activity similar to that of imipenem, although less active against pseudomonas aeruginosa. combinations of fce 22101 with ciprofloxacin or gentamicin against methicillin-resistant staphylococcus aureus and methicillin-resistant staph. epidermidis resulted in addition or indifference by the chequerboard method. the combination of fce 22101 and gentamicin against streptococcus faecalis was usually additive. against enterobacter cloacae fce 22101 ... | 1989 | 2786520 |
| in vitro activity of lomefloxacin (sc-47111) against enterobacteriaceae, enterococci and staphylococci. | the activity of lomefloxacin, a new difluorinated quinolone, was tested against 190 enterobacteriaceae strains (belonging to 23 different species), 70 enterococci and 70 staphylococci. as regards enterobacteriaceae, the activity of lomefloxacin was the same as that of norfloxacin in 9 out of the 23 species tested, and only slightly lower in further 8 species. minimum inhibitory concentrations (mic) values for 90% of strains were 0.5 microgram/ml in 2 species, 0.25 microgram/ml in 6, 0.125 microg ... | 1989 | 2786558 |
| lomefloxacin activity against 2,813 clinical isolates: a collaborative study at three medical centers in brazil. | the in vitro activity of lomefloxacin was compared to norfloxacin and two parenteral drugs against 2,813 clinical isolates at three medical centers in brazil. considering mics less than or equal to 4 micrograms/ml as the criterion, lomefloxacin inhibited 97.9% of all enterobacteriaceae, 100% of staphylococci, 100% of neisseria gonorrhoeae, 100% of campylobacter pylori, 81% of pseudomonas aeruginosa, and 79% of streptococcus spp. norfloxacin was generally comparable in potency and spectrum, but c ... | 1989 | 2791496 |
| cloning of ureaplasma urealyticum dna sequences showing genetic homology with urease genes from gram-negative bacteria. | dna chromosomic sequences from ureaplasma urealyticum were shown, by dna hybridization, to possess homology with providencia stuartii urease genes. the homologous ureaplasma dna fragment was cloned in escherichia coli and a dna probe, designated ic61 probe was specific for only the urease-producing u. urealyticum among the mollicutes. genetic homology shown between the ic61 probe and urease genes from campylobacter pylori, e. coli and p. stuartii suggests that (1) the cloned dna fragment from u. ... | 1989 | 2799066 |
| in-vitro activity of meropenem against clinical isolates obtained in canada. | the in-vitro activity of meropenem, a new parenteral carbapenem, was compared with that of imipenem, ceftazidime, cefotaxime, piperacillin, gentamicin and, where appropriate, other antibiotics against recent clinical isolates and characterized beta-lactamase producers. mics were determined by a standard agar dilution procedure and two inocula (10(4) and 10(6) cfu) were used throughout. meropenem inhibited 90% of isolates of escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-posit ... | 1989 | 2808216 |
| antibacterial activity of tigemonam dicholate (sq 30836) and interaction with beta-lactamases of gram-negative bacteria. | sq 30836 is an orally absorbed salt of tigemonam, a new monobactam similar to aztreonam in structure and microbiologic properties. when assayed against 400 clinical isolates, tigemonam's activity was similar to that of aztreonam and carumonam. it was highly effective against enterobacteriaceae but showed poor activity against gram-positive organisms. it inhibited 90% of escherichia coli, klebsiella, shigella, yersinia, proteus, providencia, and morganella strains at 0.5 micrograms/ml or less, an ... | 1989 | 2809698 |
| in vitro activity of tigemonam against multiresistant nosocomial enterobacteriaceae. | tigemonam, an oral monobactam that exhibits beta-lactamase stability similar to that of aztreonam, was tested in vitro against 240 species of enterobacteriaceae (50 escherichia coli, 48 klebsiella pneumoniae, 52 enterobacter cloacae, 32 proteus mirabilis, 22 proteus indole-positive [providencia sp.], 24 serratia sp., and 12 citrobacter sp. all strains were resistant to ampicillin and first-generation cephalosporins. in addition, 77.4% were resistant to amoxicillin plus clavulanic acid, 46.8% to ... | 1989 | 2809700 |
| in vitro studies of tigemonam: a comparison of the minimum inhibitory and minimum bactericidal concentrations (mic vs mbc). | the in vitro activity of tigemonam, a new oral monobactam, was studied with special attention to minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs). against 250 clinical isolates, it inhibited 100% of escherichia sp., klebsiella sp., serratia sp., citrobacter sp., providencia sp., proteus sp., morganella sp., aeromonas sp., yersinia sp., shigella sp., and haemophilus sp. at 0.5 mg/l or less. with 1 mg/l, 75% of enterobacter sp. were inhibited; however, three ... | 1989 | 2809701 |
| improved susceptibilities to cinoxacin and other antimicrobial agents of some providencia species re-identified on the basis of fermentation of polyhydric alcohols. | 1989 | 16312291 | |
| antibacterial properties of the bicyclic pyrazolidinones. | ly173013 and ly186826 are bicyclic pyrazolidinones containing a novel aza-gamma-lactam ring structure. the antibacterial properties of these compounds appear to be related to those of beta-lactam antibiotics in that both classes of compounds share certain common binding molecules such as beta-lactamases and penicillin-binding proteins. | 1990 | 1689710 |
| the in-vitro activity of cefodizime: a review. | for enterobacteriaceae, mic50s and mic90s of cefodizime (mg/l), respectively, were as follows, for naturally non-beta-lactamase-producing species: escherichia coli 0.12 and 0.5, salmonella spp. and shigella spp. 0.25 and 0.5, proteus mirabilis 0.016 and 0.03; for chromosomal penicillinase-producing species. klebsiella spp. 0.25 and 64, and for chromosomal cephalosporinase-producing species. enterobacter cloacae 1 and 64, citrobacter freundii 1 and 128, serratia marcescens 2 and 8: indole-positiv ... | 1990 | 2074248 |
| [antibacterial activity and beta-lactamase stability of eleven oral cephalosporins]. | oral cephalosporins (cefixime, cefdinir, cefetamet, ceftibuten, cefpodoxime, loracarbef, cefprozil, cefuroxime, cefaclor, cefadroxil and bay 3522) were compared by their antibacterial profile including stability against new beta-lactamases. both activity and antibacterial spectrum of compounds structurally related to third generation parenteral cephalosporins (of the oximino class) were superior to established compounds. activity against staphylococci was found to be highest for cefdinir, cefpro ... | 1990 | 2079378 |
| a comparison of the mechanisms of decreased susceptibility of aztreonam-resistant and ceftazidime-resistant enterobacteriaceae. | twenty five strains of enterobacteriaceae (five each of enterobacter cloacae, citrobacter freundii, serratia marcescens, morganella morganii and providencia stuartii) were exposed to aztreonam and ceftazidime at 1/2 and 1 x mic in liquid medium for 22 h, and also to 3, 5 and 10 x mic and 16 mg/l of each agent in agar. any putative mutant with an increase in the mic of greater than or equal to 4 fold was examined for beta-lactamase expression and outer membrane protein profile. mutants were selec ... | 1990 | 2081717 |
| antimicrobial activity and beta-lactamase stability of bmy-28232, parent compound of an oral cephalosporin. | bmy-28232, an aminothiazolyl imino methoxy cephalosporin which is available as an orally absorbed acetoxyethyl ester, inhibited strains of methicillin-susceptible staphylococcus aureus, hemolytic streptococci, streptococcus pneumoniae, escherichia coli, klebsiella species, and many strains of proteus and providencia stuartii at concentrations less than 1 microgram/ml, including isolates resistant to cephalexin and cefaclor. it had activity similar to that of cefixime, but was more active against ... | 1990 | 2086221 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ... | 1990 | 2086814 |
| comparative in vitro and in vivo activities of six new monofluoroquinolone and difluoroquinolone 3-carboxylic acids with a 7-azetidin ring substituent. | e-4502, e-4501, e-4500, e-4480, e-4474, and e-4441 are new monofluorinated or difluorinated quinolone agents that are chemically characterized by the presence of an azetidin ring, with different c'-3 substituents, at position 7 of the molecular structure. the mics of the difluorinated compounds e-4501, e-4474, and e-4441 for 90% of isolates were 0.06 to 1, 0.06 to 1, and 0.12 to 1 microgram/ml, respectively, against gram-positive organisms (staphylococci, streptococci, and enterococcus faecalis) ... | 1990 | 2088187 |
| combination of ofloxacin and other antimicrobial agents. | we evaluated the combination of ofloxacin with piperacillin, gentamicin, vancomycin, rifampin, clindamycin, and metronidazole by the checkerboard agar dilution method against 165 isolates which included escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, pseudomonas aeruginosa, providencia stuartii, acinetobacter, staphylococcus aureus, bacteroides fragilis, and citrobacter perfringens. synergy of piperacillin-ofloxacin was demonstrated for 50% and 40% respectivel ... | 1990 | 2093103 |
| adherence of helicobacter pylori cells and their surface components to hela cell membranes. | four helicobacter pylori strains were used to develop in vitro methods to assess adherence to hela cells. using direct detection by microscopy, adhesion scores increased with the initial bacteria-to-cell ratio. the urease method assessed h. pylori bound to hela cells by their urease activity. the percentage of the original inoculum adhering to hela cells remained constant for initial ratios from 10(2) to 10(5) bacteria per cell. an elisa using anti-h. pylori serum assessed whole bacteria or comp ... | 1990 | 2097496 |
| biological characterization of a new radioactive labeling reagent for bacterial penicillin-binding proteins. | radiolabeled penicillin g is widely used as the imaging agent in penicillin-binding protein (pbp) assays. the disadvantages of most forms of labeled penicillin g are instability on storage and the long exposure times usually required for autoradiography or fluorography of electrophoretic gels. we investigated the utility of radioiodinated penicillin v as an alternative reagent. radioiodination of p-(trimethylstannyl)penicillin v with [125i]na, using a modification of the chloramine-t method, is ... | 1990 | 2113792 |
| in-vitro and in-vivo antibacterial activities of e1040, a new cephalosporin with potent antipseudomonal activity. | e1040 is a new parenteral cephalosporin with a broad antibacterial spectrum and potent activity against gram-negative bacteria including pseudomonas aeruginosa. the in-vitro activities of e1040 against clinical isolates of enterobacter cloacae, ent. aerogenes, providencia rettgeri, and morganella morganii were superior to those of ceftazidime, cefoperazone, cefmenoxime, and cefuzonam. the activities of e1040 against gram-positive cocci were comparable with those of ceftazidime, but it was less a ... | 1990 | 2127928 |
| [in vitro activity of imipenem against hospital bacteria]. | imipenem proved highly active against enterobacteriaceae: the different bacterial groups exhibited similar mode mics (0.12 to 0.25 micrograms/ml), except for serratia (0.25-0.5 micrograms/ml), proteus mirabilis (0.5 micrograms/ml), indole-positive proteus (2 micrograms/ml) and providencia (1 mu/ml). the mics of cefotaxime-resistant strains (cephalosporinase hyperproducing or very broad spectrum betalactamase producing) were within the susceptibility range. imipenem also exhibited satisfactory ac ... | 1990 | 2139936 |
| the in-vitro activity of piperacillin/tazobactam, ciprofloxacin, ceftazidime and imipenem against multiple resistant gram-negative bacteria. | one hundred and fifty gram-negative bacterial strains including respiratory pathogens, such as haemophilus influenzae and branhamella catarrhalis, and enterobacteriaceae with known resistance to beta-lactam and other antibiotics were tested in vitro for sensitivity to piperacillin, piperacillin/tazobactam (ratio 8:1), ceftazidime, ciprofloxacin and imipenem. a 16-fold or greater reduction in the mic90 of piperacillin was achieved by the addition of tazobactam, in the respiratory pathogens, thus ... | 1990 | 2164513 |
| use of a novel phosphatase test for simplified identification of species of the tribe proteeae. | phosphatase activity of 334 isolates of the tribe proteeae carefully identified to species level has been evaluated by using both a number of conventional tests and three versions of the novel methyl green-phenolphthalein (mgp) method (g. satta. r. pompei, g. grazi, and g. cornaglia, j. clin. microbiol. 26:2637-2641, 1988). we found that the different species of proteeae show different and easily distinguishable. behaviors by the mgp method, while all of them behave in a uniform way in the conve ... | 1990 | 2166077 |
| genetic analysis of escherichia coli urease genes: evidence for two distinct loci. | studies with two uropathogenic urease-producing escherichia coli strains, 1021 and 1440, indicated that the urease genes of each are distinct. recombinant plasmids encoding urease activity from e. coli 1021 and 1440 differed in their restriction endonuclease cleavage sites and showed minimal dna hybridization under stringent conditions. the polypeptides encoded by the dna fragments containing the 1021 and 1440 urease loci differed in electrophoretic mobility under reducing conditions. regulation ... | 1990 | 2174868 |