Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| p212a mutant of dihydrodaidzein reductase enhances (s)-equol production and enantioselectivity in a recombinant escherichia coli whole-cell reaction system. | (s)-equol, a gut bacterial isoflavone derivative, has drawn great attention because of its potent use for relieving female postmenopausal symptoms and preventing prostate cancer. previous studies have reported on the dietary isoflavone metabolism of several human gut bacteria and the involved enzymes for conversion of daidzein to (s)-equol. however, the anaerobic growth conditions required by the gut bacteria and the low productivity and yield of (s)-equol limit its efficient production using on ... | 2016 | 26801575 |
| insights into mutualism mechanism and versatile metabolism of ketogulonicigenium vulgare hbe602 based on comparative genomics and metabolomics studies. | ketogulonicigenium vulgare has been widely used in vitamin c two steps fermentation and requires companion strain for optimal growth. however, the understanding of k. vulgare as well as its companion strain is still preliminary. here, the complete genome of k. vulgare hbe602 was deciphered to provide insight into the symbiosis mechanism and the versatile metabolism. k. vulgare contains the luxr family proteins, chemokine proteins, flagellar structure proteins, peptides and transporters for symbi ... | 2016 | 26979567 |
| key enzymes catalyzing glycerol to 1,3-propanediol. | biodiesel can replace petroleum diesel as it is produced from animal fats and vegetable oils, and it produces about 10 % (w/w) glycerol, which is a promising new industrial microbial carbon, as a major by-product. one of the most potential applications of glycerol is its biotransformation to high value chemicals such as 1,3-propanediol (1,3-pd), dihydroxyacetone (dha), succinic acid, etc., through microbial fermentation. glycerol dehydratase, 1,3-propanediol dehydrogenase (1,3-propanediol-oxydor ... | 2016 | 26966462 |
| transcriptomic responses of the basidiomycete yeast sporobolomyces sp. to the mycotoxin patulin. | patulin is a mycotoxin produced by penicillium expansum, the causal agent of blue mold of stored pome fruits, and several other species of filamentous fungi. this mycotoxin has genotoxic, teratogenic and immunotoxic effects in mammals, and its presence in pome fruits and derived products represents a serious health hazard. biocontrol agents in the pucciniomycotina, such as the yeasts sporobolomyces sp. strain iam 13481 and rhodosporidium kratochvilovae strain ls11, are able to resist patulin and ... | 2016 | 26956724 |
| easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles. | despite the progressive miniaturization of bioreactors for screening purposes, shake flasks are still widespread in biotechnological laboratories and industry as cultivation vessels. shake flasks are often applied as the first or second step in applications such as strain screening or media optimization. thus, there are ongoing efforts to develop online measurement techniques for shake flasks, to gain as much information as possible about the cultured microbial system. since dissolved oxygen ten ... | 2016 | 26912130 |
| reorganization of a synthetic microbial consortium for one-step vitamin c fermentation. | in the industry, the conventional two-step fermentation method was used to produce 2-keto-l-gulonic acid (2-kga), the precursor of vitamin c, by three strains, namely, gluconobacter oxydans, bacillus spp. and ketogulonicigenium vulgare. despite its high production efficiency, the long incubation period and an additional second sterilization process inhibit the further development. therefore, we aimed to reorganize a synthetic consortium of g. oxydans and k. vulgare for one-step fermentation of 2 ... | 2016 | 26809519 |
| a highly efficient sorbitol dehydrogenase from gluconobacter oxydans g624 and improvement of its stability through immobilization. | a sorbitol dehydrogenase (gosldh) from gluconobacter oxydans g624 (g. oxydans g624) was expressed in escherichia coli bl21(de3)-codonplus ril. the complete 1455-bp codon-optimized gene was amplified, expressed, and thoroughly characterized for the first time. gosldh exhibited km and kcat values of 38.9 mm and 3820 s(-1) toward l-sorbitol, respectively. the enzyme exhibited high preference for nadp(+) (vs. only 2.5% relative activity with nad(+)). gosldh sequencing, structure analyses, and bioche ... | 2016 | 27633501 |
| overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-d-gluconic acid by gluconobacter oxydans. | 2-keto-d-gluconic acid (2kga) is widely used as a chemical intermediate in the cosmetic, pharmaceutical and environmental industries. several microbial fermentation processes have been developed for production of 2kga but these suffer from substrate/product inhibition, byproduct formation and low productivity. in previous work, we showed that 2kga can be specifically produced from glucose (glu) or gluconic acid (ga) by resting wild-type gluconobacter oxydans dsm2003 cells, although substrate con ... | 2016 | 27392695 |
| vinegar production from jabuticaba (myrciaria jaboticaba) fruit using immobilized acetic acid bacteria. | cell immobilization comprises the retention of metabolically active cells inside a polymeric matrix. in this study, the production of jabuticaba (myrciaria jaboticaba) vinegar using immobilized acetobacter aceti and gluconobacter oxydans cells is proposed as a new method to prevent losses of jabuticaba fruit surplus. the pulp of jabuticaba was processed and saccharomyces cerevisiae ccma 0200 was used to ferment the must for jabuticaba wine production. sugars, alcohols (ethanol and glycerol) and ... | 2016 | 27956867 |
| pulque, a traditional mexican alcoholic fermented beverage: historical, microbiological, and technical aspects. | pulque is a traditional mexican alcoholic beverage produced from the fermentation of the fresh sap known as aguamiel (mead) extracted from several species of agave (maguey) plants that grow in the central mexico plateau. currently, pulque is produced, sold and consumed in popular districts of mexico city and rural areas. the fermented product is a milky white, viscous, and slightly acidic liquid beverage with an alcohol content between 4 and 7° gl and history of consumption that dates back to pr ... | 2016 | 27446061 |
| design and performance testing of a dna extraction assay for sensitive and reliable quantification of acetic acid bacteria directly in red wine using real time pcr. | although strategies exist to prevent aab contamination, the increased interest for wines with low sulfite addition leads to greater aab spoilage. hence, there is a real need for a rapid, specific, sensitive, and reliable method for detecting these spoilage bacteria. all these requirements are met by real time polymerase chain reaction (or quantitative pcr; qpcr). here, we compare existing methods of isolating dna and their adaptation to a red wine matrix. two different protocols for isolating dn ... | 2016 | 27313572 |
| 16s rrna in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production. | acetic acid bacteria are involved in many biotechnological processes such as vitamin c, gluconic acid, miglitol or acetic acid production, and others. for a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. during the past few years hybridization in a combination with flow cytometry has often been used for this purpose. since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. in ... | 2016 | 27904400 |
| fermentative production of high titer gluconic and xylonic acids from corn stover feedstock by gluconobacter oxydans and techno-economic analysis. | high titer gluconic acid and xylonic acid were simultaneously fermented by gluconobacter oxydans dsm 2003 using corn stover feedstock after dry dilute sulfuric acid pretreatment, biodetoxification and high solids content hydrolysis. maximum sodium gluconate and xylonate were produced at the titer of 132.46g/l and 38.86g/l with the overall yield of 97.12% from glucose and 90.02% from xylose, respectively. the drawbacks of filamentous fungus aspergillus niger including weak inhibitor tolerance, la ... | 2016 | 27484668 |
| whole-genome sequence analysis of bombella intestini lmg 28161t, a novel acetic acid bacterium isolated from the crop of a red-tailed bumble bee, bombus lapidarius. | the whole-genome sequence of bombella intestini lmg 28161t, an endosymbiotic acetic acid bacterium (aab) occurring in bumble bees, was determined to investigate the molecular mechanisms underlying its metabolic capabilities. the draft genome sequence of b. intestini lmg 28161t was 2.02 mb. metabolic carbohydrate pathways were in agreement with the metabolite analyses of fermentation experiments and revealed its oxidative capacity towards sucrose, d-glucose, d-fructose and d-mannitol, but not eth ... | 2016 | 27851750 |
| crystal structure of pyruvate decarboxylase from zymobacter palmae. | pyruvate decarboxylase (pdc; ec 4.1.1.1) is a thiamine pyrophosphate- and mg(2+) ion-dependent enzyme that catalyses the non-oxidative decarboxylation of pyruvate to acetaldehyde and carbon dioxide. it is rare in bacteria, but is a key enzyme in homofermentative metabolism, where ethanol is the major product. here, the previously unreported crystal structure of the bacterial pyruvate decarboxylase from zymobacter palmae is presented. the crystals were shown to diffract to 2.15 å resolution. they ... | 2016 | 27599861 |
| heterologous overexpression and biochemical characterization of a nitroreductase from gluconobacter oxydans 621h. | a nadph-dependent and fmn-containing nitroreductase (gox0834) from gluconobacter oxydans was cloned and heterogeneously expressed in escherichia coli. the purified enzyme existed as a dimer with an apparent molecular mass of about 31.4 kda. the enzyme displayed broad substrate specificity and reduced a variety of mononitrated, polynitrated, and polycyclic nitroaromatic compounds to the corresponding amino products. the highest activity was observed for the reduction of cb1954 (5-(1-aziridinyl)-2 ... | 2016 | 27138989 |
| a single membrane-bound enzyme catalyzes the conversion of 2,5-diketo-d-gluconate to 4-keto-d-arabonate in d-glucose oxidative fermentation by gluconobacter oxydans nbrc 3292. | 4-keto-d-arabonate synthase (4kas), which converts 2,5-diketo-d-gluconate (dkga) to 4-keto-d-arabonate (4ka) in d-glucose oxidative fermentation by some acetic acid bacteria, was solubilized from the gluconobacter oxydans nbrc 3292 cytoplasmic membrane, and purified in an electrophoretically homogenous state. a single membrane-bound enzyme was found to catalyze the conversion from dkga to 4ka. the 92-kda 4kas was a homodimeric protein not requiring o2 or a cofactor for the conversion, but was st ... | 2016 | 27010909 |
| enhanced xylitol production: expression of xylitol dehydrogenase from gluconobacter oxydans and mixed culture of resting cell. | xylitol has numerous applications in food and pharmaceutical industry, and it can be biosynthesized by microorganisms. in the present study, xdh gene, encoding xylitol dehydrogenase (xdh), was cloned from the genome of gluconobacter oxydans cgmcc 1.49 and overexpressed in escherichia coli bl21. sequence analysis revealed that xdh has a tgxxgxxg nad(h)-binding motif and a yxxxk active site motif, and belongs to the short-chain dehydrogenase/reductase family. and then, the enzymatic properties and ... | 2016 | 26975753 |
| extracellular targeting of an active endoxylanase by a tolb negative mutant of gluconobacter oxydans. | gluconobacter (g.) oxydans strains have great industrial potential due to their ability to incompletely oxidize a wide range of carbohydrates. but there is one major limitation preventing their full production potential. hydrolysis of polysaccharides is not possible because extracellular hydrolases are not encoded in the genome of gluconobacter species. therefore, as a first step for the generation of exoenzyme producing g. oxydans, a leaky outer membrane mutant was created by deleting the tolb ... | 2016 | 27097633 |
| comparison of d-gluconic acid production in selected strains of acetic acid bacteria. | the oxidative metabolism of acetic acid bacteria (aab) can be exploited for the production of several compounds, including d-gluconic acid. the production of d-gluconic acid in fermented beverages could be useful for the development of new products without glucose. in the present study, we analyzed nineteen strains belonging to eight different species of aab to select those that could produce d-gluconic acid from d-glucose without consuming d-fructose. we tested their performance in three differ ... | 2016 | 26848948 |
| enhancement of 1,3-dihydroxyacetone production from gluconobacter oxydans by combined mutagenesis. | wild strain l-6 was subjected to combined mutagenesis, including uv irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (dha). with application of a high-throughput screening method, mutant gluconobacter oxydans i-2-239 with a dha productivity of 103.5 g/l in flask-shake fermentation was finally obtained with the starting glycerol concentration of 120 g/l, which was 115.7% higher than the wild strain. the cultivation time ... | 2016 | 27876710 |
| enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in gluconobacter oxydans dsm 2003. | membrane-bound alcohol dehydrogenase (madh) was overexpressed in gluconobacter oxydans dsm 2003, and the effects on cell growth and glycolic acid production were investigated. the transcription levels of two terminal ubiquinol oxidases (bo3 and bd) in the respiratory chain of the engineered strain g. oxydans-adhabs were up-regulated by 13.4- and 3.8-fold, respectively, which effectively enhanced the oxygen uptake rate, resulting in higher resistance to acid. the cell biomass of g. oxydans-adhabs ... | 2016 | 27619641 |
| kinetic analysis of dihydroxyacetone production from crude glycerol by immobilized cells of gluconobacter oxydans mtcc 904. | the present study has investigated kinetic features of bioconversion of biodiesel-derived crude glycerol to dihydroxyacetone with immobilized gluconobacter oxydans cells using modified haldane substrate-inhibition model. the results have been compared against free cells and pure glycerol. relative variations in the kinetic parameters ks, ki, vmax, n and x reveal that immobilized g. oxydans cells (on pu foam substrate) with crude glycerol as substrate give higher order of inhibition (n) and lower ... | 2016 | 27343447 |
| role of mannitol dehydrogenases in osmoprotection of gluconobacter oxydans. | gluconobacter (g.) oxydans is able to incompletely oxidize various sugars and polyols for the production of biotechnologically important compound. recently, we have shown that the organism produces and accumulates mannitol as compatible solute under osmotic stress conditions. the present study describes the role of two cytoplasmic mannitol dehydrogenases for osmotolerance of g. oxydans. it was shown that gox1432 is a nadp(+)-dependent mannitol dehydrogenase (ec 1.1.1.138), while gox0849 uses nad ... | 2016 | 27338577 |
| a new homozygous frameshift mutation in the hsd3b2 gene in an apparently nonconsanguineous italian family. | 3β-hydroxysteroid dehydrogenase (3β-hsd) deficiency is a rare cause of congenital adrenal hyperplasia (cah) caused by inactivating mutations in the hsd3b2 gene. | 2016 | 27082427 |
| improving the production yield and productivity of 1,3-dihydroxyacetone from glycerol fermentation using gluconobacter oxydans nl71 in a compressed oxygen supply-sealed and stirred tank reactor (cos-sstr). | in this study, a compressed oxygen gas supply was connected to a sealed aerated stirred tank reactor (cos-sstr) bio-system, leading to a high-oxygen pressure bioreactor used to improve the bio-transformative performance in the production of 1,3-dihydroxyacetone (dha) from glycerol using gluconobacter oxydans nl71. a concentration of 301.2 ± 8.2 g l(-1) dha was obtained from glycerol after 32 h of fed-batch fermentation in the cos-sstr system. the volumetric productivity for this process was 9.41 ... | 2016 | 27021347 |
| effective improvement of the activity of membrane-bound alcohol dehydrogenase by overexpression of adhs in gluconobacter oxydans. | to investigate the roles of adhs, which encodes the adhs subunit of membrane-bound alcohol dehydrogenase (madh) in gluconobacter oxydans dsm2003, and to rationally improve madh activity. | 2016 | 27015861 |
| enhancement of 5-keto-d-gluconate production by a recombinant gluconobacter oxydans using a dissolved oxygen control strategy. | the rapid and incomplete oxidation of sugars, alcohols, and polyols by the gram-negative bacterium gluconobacter oxydans facilitates a wide variety of biological applications. for the conversion of glucose to 5-keto-d-gluconate (5-kga), a promising precursor of the industrial substance l-(+)-tartaric acid, g. oxydans dsm2343 was genetically engineered to strain zju2, in which the gox1231 and gox1081 genes were knocked out in a markerless fashion. then, a secondary alcohol dehydrogenase (gcd) fro ... | 2016 | 26896860 |
| optimization of 1,3-dihydroxyacetone production from crude glycerol by immobilized gluconobacter oxydans mtcc 904. | this study has addressed the matter of optimization of production of the value added product, dihydroxyacetone, from crude glycerol using immobilized cells of gluconobacter oxydans. statistical optimization of the fermentation medium revealed mgso4·7h2o, (nh4)2so4 and kh2po4 as the significant components, in addition to small concentration of yeast extract. as per previous literature, these components augment the activity of glycerol dehydrogenase enzyme in metabolism and provide assimilable nit ... | 2016 | 26873288 |
| identification of an interaction between ei and a histidine kinase-response regulator hybrid protein in gluconobacter oxydans. | gluconobacter oxydans may contain an incomplete phosphoenolpyruvate: carbohydrate phosphotransferase system consisting of three components--ei, hpr and eiia, while the function of individual members of the system remains unknown. in this research, a specific interaction between ei and a histidine kinase-response regulator hybrid protein was screened by yeast two-hybrid assay, and the interaction was further identified with gst pull-down assay and bimolecular fluorescence complementation assay in ... | 2016 | 26792729 |
| improved xylitol production from d-arabitol by enhancing the coenzyme regeneration efficiency of the pentose phosphate pathway in gluconobacter oxydans. | gluconobacter oxydans is used to produce xylitol from d-arabitol. this study aims to improve xylitol production by increasing the coenzyme regeneration efficiency of the pentose phosphate pathway in g. oxydans. glucose-6-phosphate dehydrogenase (g6pdh) and 6-phosphogluconate dehydrogenase (6pgdh) were overexpressed in g. oxydans. real-time pcr and enzyme activity assays revealed that g6pdh/6pgdh activity and coenzyme regeneration efficiency increased in the recombinant g. oxydans strains. approx ... | 2016 | 26727541 |
| towards the computational design and engineering of enzyme enantioselectivity: a case study by a carbonyl reductase from gluconobacter oxydans. | in our previous work, a nad(h)-dependent carbonyl reductase (gocr) was identified from gluconobacter oxydans, which showed moderate to high enantiospecificity for the reduction of different kinds of prochiral ketones. in the present study, the crystal structure of gocr was determined at 1.65å resolution, and a computational strategy concerning substrate-enzyme docking and all-atom molecular dynamics (md) simulation was established to help understand the molecular basis of enantiopreference and e ... | 2016 | 26590330 |
| assessing the stereoselectivity of carbonyl reductases toward the reduction of opbe and docking analysis. | the asymmetric reduction of prochiral carbonyl compounds by nad(p)h-dependent carbonyl reductases represents a powerful method for the production of optically active alcohols. the stereoselectivity of a series of carbonyl reductases were evaluated toward the reduction of ethyl 2-oxo-4-phenylbutyrate (opbe). a majority of reductases produced the ethyl (r)-2-hydroxy-4-phenylbutyrate ((r)-hpbe) with low to excellent enantiomeric excess (e.e.), whereas about 30% reductases catalyzed opbe to form (s) ... | 2016 | 25989134 |
| characterization of cross-linked immobilized arylesterase from gluconobacter oxydans 621h with activity toward cephalosporin c and 7-aminocephalosporanic acid. | cross-linked enzyme aggregates (cleas) were prepared from several precipitant agents using glutaraldehyde as a cross-linking agent with and without bsa, finally choosing a 40% saturation of ammonium sulfate and 25 mm of glutaraldehyde. the cleas obtained under optimum conditions were biochemically characterized. the immobilized enzyme showed higher thermal activity and a broader range of ph and organic solvent tolerance than the free enzyme. arylesterase from gluconobacter oxydans showed activit ... | 2016 | 26443058 |
| simultaneous bioconversion of xylose and glycerol to xylonic acid and 1,3-dihydroxyacetone from the mixture of pre-hydrolysates and ethanol-fermented waste liquid by gluconobacter oxydans. | simultaneous bioconversion of xylose and glycerol to xylonic acid and 1,3-dihydroxyacetone (dha) was realized by using gluconobacter oxydans (g. oxydans). currently, the enzymatic hydrolysate to ethanol-fermented waste liquid and the inorganic acid pre-hydrolysate that contain abundant glycerol and xylose were difficult to be utilized or disposed. based on the method of compressed oxygen supply-sealed and stirred tank reactor system (cos-sstr), the xylonic acid and 1,3-dihydroxyacetone could be ... | 2016 | 26378011 |
| regulation of pyrroloquinoline quinone-dependent glucose dehydrogenase activity in the model rhizosphere-dwelling bacterium pseudomonas putida kt2440. | soil-dwelling microbes solubilize mineral phosphates by secreting gluconic acid, which is produced from glucose by a periplasmic glucose dehydrogenase (gdh) that requires pyrroloquinoline quinone (pqq) as a redox coenzyme. while gdh-dependent phosphate solubilization has been observed in numerous bacteria, little is known concerning the mechanism by which this process is regulated. here we use the model rhizosphere-dwelling bacterium pseudomonas putida kt2440 to explore gdh activity and pqq synt ... | 2016 | 27287323 |
| determinants of cofactor specificity for the glucose-6-phosphate dehydrogenase from escherichia coli: simulation, kinetics and evolutionary studies. | glucose 6-phosphate dehydrogenases (g6pdhs) from different sources show varying specificities towards nad+ and nadp+ as cofactors. however, it is not known to what extent structural determinants of cofactor preference are conserved in the g6pdh family. in this work, molecular simulations, kinetic characterization of site-directed mutants and phylogenetic analyses were used to study the structural basis for the strong preference towards nadp+ shown by the g6pdh from escherichia coli. molecular dy ... | 2016 | 27010804 |
| comparative transcriptomics across the prokaryotic tree of life. | whole-transcriptome sequencing studies from recent years revealed an unexpected complexity in transcriptomes of bacteria and archaea, including abundant non-coding rnas, cis-antisense transcription and regulatory untranslated regions (utrs). understanding the functional relevance of the plethora of non-coding rnas in a given organism is challenging, especially since some of these rnas were attributed to 'transcriptional noise'. to allow the search for conserved transcriptomic elements we produce ... | 2016 | 27154273 |
| review: diversity of microorganisms in global fermented foods and beverages. | culturalable and non-culturable microorganisms naturally ferment majority of global fermented foods and beverages. traditional food fermentation represents an extremely valuable cultural heritage in most regions, and harbors a huge genetic potential of valuable but hitherto undiscovered strains. holistic approaches for identification and complete profiling of both culturalable and non-culturable microorganisms in global fermented foods are of interest to food microbiologists. the application of ... | 2016 | 27047484 |
| comparative genomic and functional analysis reveal conservation of plant growth promoting traits in paenibacillus polymyxa and its closely related species. | paenibacillus polymyxa has widely been studied as a model of plant-growth promoting rhizobacteria (pgpr). here, the genome sequences of 9 p. polymyxa strains, together with 26 other sequenced paenibacillus spp., were comparatively studied. phylogenetic analysis of the concatenated 244 single-copy core genes suggests that the 9 p. polymyxa strains and 5 other paenibacillus spp., isolated from diverse geographic regions and ecological niches, formed a closely related clade (here it is called poly- ... | 2016 | 26856413 |
| repeated biotransformation of glycerol to 1,3-dihydroxyacetone by immobilized cells of gluconobacter oxydans with glycerol- and urea-feeding strategy in a bubble column bioreactor. | some inorganic nitrogen sources and amino acids instead of yeast extract, which resulted in trouble of product purification, were introduced for 1,3-dihydroxyacetone (dha) production by biotransformation with gluconobacter oxydans. the results showed that urea is an optimal nitrogen source. furthermore, the effects of glycerol- and urea-feeding strategies for dha production by immobilized cells in a home-made bubble column bioreactor were optimized. cells immobilization was prepared by cultivati ... | 2017 | 28279907 |
| characterization of membrane-bound dehydrogenases of gluconobacter oxydans 621h using a new system for their functional expression. | acetic acid bacteria are used in biotechnology due to their ability to incompletely oxidize a great variety of carbohydrates, alcohols, and related compounds in a regio- and stereo-selective manner. these reactions are catalyzed by membrane-bound dehydrogenases (mdhs), often with a broad substrate spectrum. in this study, the promoters of six mdhs of gluconobacter oxydans 621h were characterized. the constitutive promoter of the alcohol dehydrogenase and the glucose-repressed promoter of the ino ... | 2017 | 28064365 |
| oxidative production of xylonic acid using xylose in distillation stillage of cellulosic ethanol fermentation broth by gluconobacter oxydans. | an oxidative production process of xylonic acid using xylose in distillation stillage of cellulosic ethanol fermentation broth was designed, experimentally investigated, and evaluated. dry dilute acid pretreated and biodetoxified corn stover was simultaneously saccharified and fermented into 59.80g/l of ethanol (no xylose utilization). 65.39g/l of xylose was obtained in the distillation stillage without any concentrating step after ethanol was distillated. then the xylose was completely converte ... | 2017 | 27955866 |
| co-production of hydrogen and ethanol from glucose in escherichia coli by activation of pentose-phosphate pathway through deletion of phosphoglucose isomerase (pgi) and overexpression of glucose-6-phosphate dehydrogenase (zwf) and 6-phosphogluconate dehydrogenase (gnd). | biologically, hydrogen (h2) can be produced through dark fermentation and photofermentation. dark fermentation is fast in rate and simple in reactor design, but h2 production yield is unsatisfactorily low as <4 mol h2/mol glucose. to address this challenge, simultaneous production of h2 and ethanol has been suggested. co-production of ethanol and h2 requires enhanced formation of nad(p)h during catabolism of glucose, which can be accomplished by diversion of glycolytic flux from the embden-meyer ... | 2017 | 28360941 |
| identification of acetic acid bacteria through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and report of gluconobacter nephelii kommanee et al. 2011 and gluconobacter uchimurae tanasupawat et al. 2012 as later heterotypic synonyms of gluconobacter japonicus malimas et al. 2009 and gluconobacter oxydans (henneberg 1897) de ley 1961 (approved lists 1980) emend. gosselé et al. 1983, respectively. | the classification and identification of acetic acid bacteria (aab) underwent much change during the last two decades. however, several closely related aab species are difficult to differentiate and rapid and correct species level identification remains challenging. in the present study, 281 well-characterized aab strains representing aab species of the family acetobacteraceae were used to construct a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) ide ... | 2017 | 28237188 |
| effect of ammonium and amino acids on the growth of selected strains of gluconobacter and acetobacter. | acetic acid bacteria (aab) are a group of microorganisms highly used in the food industry. however, its use can be limited by the insufficient information known about the nutritional requirements of aab for optimal growth. the aim of this work was to study the effects of different concentrations and sources of nitrogen on the growth of selected aab strains and to establish which nitrogen source best encouraged their growth. two strains of three species of aab, gluconobacter japonicus, gluconobac ... | 2017 | 27870985 |
| reconstruction of amino acid biosynthetic pathways increases the productivity of 2-keto-l-gulonic acid in ketogulonicigenium vulgare-bacillus endophyticus consortium via genes screening. | defect in the amino acid biosynthetic pathways of ketogulonicigenium vulgare, the producing strain for 2-keto-l-gulonic acid (2-kga), is the key reason for its poor growth and low productivity. in this study, five different strains were firstly reconstructed by expressing absent genes in threonine, proline and histidine biosynthetic pathways for better 2-kga productivity. when mono-cultured in the shake flasks, the strain sybe_kv02080002 expressing hsk from gluconobacter oxydans in threonine bio ... | 2017 | 28283955 |
| highly efficient bioreduction of 2-hydroxyacetophenone to (s)- and (r)-1-phenyl-1,2-ethanediol by two substrate tolerance carbonyl reductases with cofactor regeneration. | optically pure 1-phenyl-1,2-ethanediol is a very important chiral building block and intermediate in fine chemical and pharmaceutical industries. reduction of 2-hydroxyacetophenone provides a straightforward approach to access these important compounds. in this study, two enantiocomplementary carbonyl reductases, bdha (2,3-butanediol dehydrogenase from bacillus subtilis) and goscr (polyol dehydrogenase from gluconobacter oxydans) were discovered for the first time to convert 2-hydroxyacetophenon ... | 2017 | 28011130 |
| global analysis of mannitol 2-dehydrogenase in lactobacillus reuteri crl 1101 during mannitol production through enzymatic, genetic and proteomic approaches. | several plants, fungi, algae, and certain bacteria produce mannitol, a polyol derived from fructose. mannitol has multiple industrial applications in the food, pharmaceutical, and medical industries, being mainly used as a non-metabolizable sweetener in foods. many heterofermentative lactic acid bacteria synthesize mannitol when an alternative electron acceptor such as fructose is present in the medium. in previous work, we reported the ability of lactobacillus reuteri crl 1101 to efficiently pr ... | 2017 | 28060932 |