Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the primary structure and structural characteristics of achromobacter lyticus protease i, a lysine-specific serine protease. | the complete amino acid sequence of achromobacter lyticus protease i (ec 3.4.21.50), which specifically hydrolyzes lysyl peptide bonds, has been established. this has been achieved by sequence analysis of the reduced and s-carboxymethylated protease and of peptides obtained by enzymatic digestion with achromobacter protease i itself and staphylococcus aureus v8 protease and by chemical cleavage with cyanogen bromide. the protease consists of 268 residues with three disulfide bonds, which have be ... | 1989 | 2492988 |
| molecular relationship of chromosomal genes encoding biphenyl/polychlorinated biphenyl catabolism: some soil bacteria possess a highly conserved bph operon. | all the genes we examined that encoded biphenyl/polychlorinated biphenyl (pcb) degradation were chromosomal, unlike many other degradation-encoding genes, which are plasmid borne. the molecular relationship of genes coding for biphenyl/pcb catabolism in various biphenyl/pcb-degrading pseudomonas, achromobacter, alcaligenes, moraxella, and arthrobacter strains was investigated. among 15 strains tested, 5 pseudomonas strains and one alcaligenes strain possessed the bphabc gene cluster on the xhoi ... | 1989 | 2507526 |
| spectroscopic evidence for a copper-nitrosyl intermediate in nitrite reduction by blue copper-containing nitrite reductase. | the reactions of nitrogen monoxide (no) with the blue copper-containing nitrite reductases from alcaligenes sp. ncib 11015 and achromobacter cycloclastes iam 1013 were investigated spectroscopically. the electron paramagnetic resonance (epr) signals of the blue coppers vanished in the presence of no at 77 k, being fully restored by the removal of no. the additions of no to the enzyme solutions resulted in the substantial bleaching of the visible absorption bands at room temperature. the reaction ... | 1989 | 2556127 |
| action of a bacterial achromobacter collagenase on the soft carious dentine: an in vitro study with the scanning electron microscope. | samples of carious human teeth treated in vitro for 48-92 hours with a bacterial achromobacter collagenase in solution in borate buffer at 33 degrees were examined with the scanning electron microscope. the enzyme was seen to destroy soft carious dentine but not sound layers of dentine beneath the lesion. this finding may have clinical implications. | 1989 | 2559081 |
| asp700i, a novel isoschizomer of xmni from achromobacter species 700 recognizing 5'-gaann/nnttc-3'. | 1989 | 2587236 | |
| asphi, a novel isoschizomer of hgiai from achromobacter species h recognizing 5'-gwgcw/c-3'. | 1989 | 2587281 | |
| fatal neonatal meningitis and ventriculitis caused by multiresistant achromobacter xylosoxidans. a case report. | meningitis and ventriculitis in a 6-day-old neonate caused by a gram-negative glucose-non-fermenting organism, achromobacter xylosoxidans, was resistant to most antibiotics except ceftazidime and imipenem. the organism became resistant after 28 days treatment with ceftazidime. when the infant was 7 weeks old, imipenem became available but, in spite of 3 days of intravenous treatment, the organism was still recovered from ventricular cerebrospinal fluid and the child died. this would appear to be ... | 1989 | 2588089 |
| immunological identification and distribution of dissimilatory heme cd1 and nonheme copper nitrite reductases in denitrifying bacteria. | polyclonal antibodies were used to identify heme or copper nitrite reductases in the following groups: 23 taxonomically diverse denitrifiers from culture collections, 100 numerically dominant denitrifiers from geographically diverse environments, and 51 denitrifiers from a culture collection not selected for denitrification. antisera were raised against heme nitrite reductases from pseudomonas aeruginosa and pseudomonas stutzeri and against copper nitrite reductase from achromobacter cycloclaste ... | 1989 | 2624465 |
| extracellular product of nocardia amarae induces bacterial cell flocculation. | the fact that nocardia amarae yk1 produced a bacterial flocculation-inducing substance (designated as fix) was discovered. fix had a function of flocculating proliferous cells. fix-induced flocculation was inhibited by making cells resting, but not completely by adding chloramphenicol. fix worked widely on gram-positive to -negative bacteria. in the presence of fix, achromobacter cycloclastus iam1013, acinetobacter calcoaceticus iam1517, bacillus subtilis iam1069, escherichia coli c600-1, e. col ... | 1989 | 2653957 |
| cloning of a carbofuran hydrolase gene from achromobacter sp. strain wm111 and its expression in gram-negative bacteria. | a 14-kilobase-pair (kbp) ecori dna fragment that encodes an enzyme capable of rapid hydrolysis of n-methylcarbamate insecticides (carbofuran hydrolase) was cloned from carbofuran-degrading achromobacter sp. strain wm111. when used to probe southern blots containing plasmid and total dnas from wm111, this 14-kbp fragment hybridized strongly to a 14-kbp ecori fragment from the greater than 100-kbp plasmid harbored by this strain but weakly to ecori-digested total dna from achromobacter sp. strain ... | 1989 | 2661544 |
| [catalytic component of calmodulin-independent adenylate cyclase from bovine brain. isolation and determination of partial amino acid sequence]. | the catalytic component of calmodulin-independent adenylate cyclase of cattle cerebral cortex was solubilized and purified to the homogeneous state. the conditions for preparative obtaining of the enzyme on the column with immobilized antibodies to adenylate cyclase were found. these antibodies were proved to interact with the calmodulin-independent rather than the calmodulin-dependent form of the enzyme. molecular mass of the calmodulin-independent adenylate cyclase determined electrophoretical ... | 1989 | 2662976 |
| cloning, nucleotide sequence, and expression of achromobacter protease i gene. | achromobacter protease i (api) is a lysine-specific serine protease which hydrolyzes specifically the lysyl peptide bond. a gene coding for api was cloned from achromobacter lyticus m497-1. nucleotide sequence of the cloned dna fragment revealed that the gene coded for a single polypeptide chain of 653 amino acids. the n-terminal 205 amino acids, including signal peptide and the threonine/serine-rich c-terminal 180 amino acids are flanking the 268 amino acid-mature protein which was identified b ... | 1989 | 2684982 |
| the primary structure of porcine liver acylamino acid-releasing enzyme deduced from cdna sequences. | two overlapping cloned cdnas encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (aare) [ec 3.4.19.1] have been isolated from porcine liver cdna lambda gt10 cdna libraries and sequenced. sequence analyses of the cdna and several achromobacter protease i-digested peptides of the purified protein revealed that porcine liver aare consists of four identical subunits, and each comprising a single chain of 732 amino acids with acetylmethionine at the n-terminus. | 1989 | 2691504 |
| determination of partial amino acid sequence of lipoate acetyltransferase of rat pyruvate dehydrogenase complex. | the partial amino acid sequence of rat lipoate acetyltransferase was determined using the intact protein and the peptides derived from a digest with achromobacter protease i. the results showed the amino-terminal sequence of the mature enzyme to be (n) ser-leu-pro-pro-his-gln-lys-val-pro-leu-pro-ser- leu-ser-pro-thr-met-gln-ala-gly-thr-ile-ala-arg-trp-glu-lys. in addition, the sequences of two possible lipoyl-binding sites in the subunit, which are very similar to each other, were established. | 1989 | 2696958 |
| [production of antibiotic substances by natural variants of the marine bacterium vibrio fischeri]. | it was shown that under definite conditions there was competition between natural variants of sea bacteria belonging to v. fischeri. natural variants of v. fischeri, strain 6 differed in their resistance to streptomycin and had different growth rates under conditions of limited aeration. morphologically all the variants were identical. v. fischeri p-0, v. fischeri p-1 and v. fischeri p-2 were studied. the study revealed that v. fischeri p-0 produced a non-dialysing thermostable trypsin-sensitive ... | 1989 | 2730220 |
| comparative studies on the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes. | we have studied the primary structure of human cystatin as from epidermis, liver, spleen, and leukocytes. these molecules were indistinguishable on page in the presence and absence of sds, by fast protein liquid chromatography (fplc) chromatofocusing on a mono p column, and in amino acid composition. the nh2- and cooh-terminal amino acid sequences of human cystatin as from epidermis, liver, and spleen were identical with those of human leukocyte cystatin a previously reported except for the lack ... | 1989 | 2768224 |
| application of gas-liquid chromatography to the routine identification of nonfermenting gram-negative bacteria in clinical specimens. | a total of 430 strains of glucose-nonfermenting gram-negative bacteria representing 35 species were analyzed for their cellular fatty acid composition by gas-liquid chromatography (glc). on the basis of qualitative differences in their cellular fatty acid composition, these bacteria could be divided into 19 distinct chromatographic groups. eight pseudomonas species, achromobacter xylosoxidans, group vd, and agrobacterium radiobacter were identified from their fatty acid compositions alone. the o ... | 1989 | 2768442 |
| amino acid sequence of alcohol dehydrogenase from the thermophilic bacterium thermoanaerobium brockii. | the complete amino acid sequence of alcohol dehydrogenase of thermoanaerobium brockii (tbad) is presented. the s-carboxymethylated protein was cleaved at methionine residues (with cyanogen bromide) to provide a set of 10 nonoverlapping fragments accounting for 90% of the sequence. these fragments were then overlapped and aligned, and the sequence was completed by using peptides generated by proteolytic cleavage at lysine residues (with achromobacter protease i). the protein subunit contained 352 ... | 1989 | 2790012 |
| achromobacter xylosoxidans corneal ulcer in a therapeutic soft contact lens wearer. | achromobacter xylosoxidans is an opportunistic organism that is usually seen in immunocompromised or immunosuppressed patients. it is an aerobic gram-negative rod, often confused with other more commonly seen gram-negative bacteria such as pseudomonas aeruginosa. the organism is usually sensitive to extended spectrum penicillins such as carbenicillin and usually resistant to aminoglycosides and first generation cephalosporins. we wish to describe a corneal ulcer from a. xylosoxidans that develop ... | 1989 | 2805715 |
| [role of a collagenase in the latent proteolytic activity of fibronectin]. | fibronectin fragments generated by achromobacter iophagus collagenase exhibit a gelatinolytic activity. this activity is inhibited by phenyl-methyl-sulfonyl fluoride and pepstatin a. after separation of this collagenase digest of fibronectin on heparin ultrogel, a laminase activity was also evidenced using laminin and the synthetic peptide gly-pro-ala-gly-pro-arg as substrates. different results were obtained with a cathepsin d digest of fibronectin that exhibited gelatinolytic and laminolytic a ... | 1990 | 1963217 |
| absence of role for plasmids in resistance to multiple disinfectants in three strains of bacteria. | three chlorhexidine-resistant strains, two of achromobacter xylosoxidans, one of pseudomonas cepacia, were isolated from clinical sources. the three strains showed cross-resistance to benzethonium chloride, benzalkonium chloride and alkyldiaminoethylglycine. resistance persisted after treatment of the strains with acridine orange (12.5 mg-25 mg l-1). conjugation to appropriate recipients of escherichia coli was unsuccessful and plasmid dna could not be detected. these results suggest that plasmi ... | 1990 | 1969437 |
| limited proteolysis of silkworm antitrypsin by several proteinases. | silkworm antitrypsin (sw-at) isolated from larval hemolymph was limitedly digested by achromobacter lysylendopeptidase, alpha-chymotrypsin, subtilisin bpn', subtilisin carlsberg, papain, or pseudomonas elastase. each proteinase could cleave specific site(s) around the reactive site identified for the reaction of sw-at and bovine trypsin. among these proteinases, only subtilisin bpn' was inhibited by sw-at, although weakly. by the cleavable amino acid sequence in sw-at, it was suggested that whet ... | 1990 | 1368515 |
| cloning and sequence analysis of cdna for trimeresurus flavoviridis phospholipase a2, and consequent revision of the amino acid sequence. | a cdna clone of trimeresurus flavoviridis phospholipase a2 was isolated from the venom gland cdna library using dna amplified by polymerase chain reaction with oligonucleotide primers corresponding to the n- and c-terminal sequences of this enzyme. the amino acid sequence deduced from the cdna nucleotide sequence determined by the dideoxy termination method was found to be inconsistent in the segment comprising the 69th to 81st positions from that reported previously [tanaka, s. et al. (1986) j. ... | 1990 | 1707052 |
| enzymatic semisynthesis of human insulin: an update. | peptide bond formation can be enzymatically catalysed by the reverse reaction of proteases. application is seen in the industrial production of human insulin. human insulin derivative can be enzymatically prepared using either porcine insulin or the single chain b(1-29)-a(1-21) insulin precursor as the starting material. this is accomplished by either (1) digesting the starting material at lys29 with achromobacter lyticus protease i (ach) and then coupling with thr-x (x = blocking residue) (two- ... | 1990 | 2096884 |
| antimicrobial activity of ro 24-6778, a covalent bonding of desmethylfleroxacin and desacetylcefotaxime. | a new "dual action" cephalosporin (ro 24-6778), representing an ester-linked desacetylcefotaxime and desmethylfleroxacin was tested against 287 aerobic bacteria. ro 24-6778 was found to be very active (minimal inhibitory concentrations [mic90], less than or equal to 0.5 micrograms/ml) against enterobacteriaceae, streptococcus spp., and aeromonas hydrophila. moderate ro 24-6778 activity (mic90, 1-8 micrograms/ml) was demonstrated against bacillus spp., staphylococcus spp. (including oxacillin-res ... | 1990 | 2116952 |
| identification of lysine 134 in the steroid-binding site of the sex steroid-binding protein of human plasma. | the sex steroid-binding protein of human plasma sbp (or sex hormone-binding globulin, shbg) was specifically inhibited with the alkylating affinity label, 17 beta-[[( 2-14c]bromoacetyl)oxy]-5 alpha-androstan-3-one. the natural ligand, 5 alpha-dihydrotestosterone, was shown to protect against inactivation and labeling. the steroid-binding activity of the protein was abolished when approximately 1 mol of label was incorporated into 1 mol of dimeric sbp. in order to identify and locate the labeled ... | 1990 | 2120231 |
| inhibitory effect of halocyamine, an antimicrobial substance from ascidian hemocytes, on the growth of fish viruses and marine bacteria. | halocyamine a, an antimicrobial substance isolated from hemocytes of the solitary ascidian halocynthia roretzi, inhibited in vitro the growth of fish rna viruses (infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus). pretreatment of rna virus with halocyamine a reduced the infectivity of the virus toward host cells. the growth of marine bacteria, achromobacter aquamarinus and pseudomonas perfectomarinus, was also inhibited by halocyamine a but that of alteromonas put ... | 1990 | 2121517 |
| posttranslationally processed structure of the human platelet protein smg p21b: evidence for geranylgeranylation and carboxyl methylation of the c-terminal cysteine. | smg p21a and -b are small gtp-binding proteins that share putative effector and consensus c-terminal sequences with ras p21 proteins. in the present report, we showed that human platelet smg p21b became labeled when intact platelets were incubated with exogenous [3h]mevalonolactone and when a purified preparation of smg p21b was incubated with bovine brain membranes and s-adenosyl-l-[methyl-3h]methionine. in addition, we demonstrated by gas chromatography/mass spectrometry that treatment of smg ... | 1990 | 2123345 |
| isolation of a high specific activity pink, monomeric nitrous oxide reductase from achromobacter cycloclastes. | nitrous oxide reductase from achromobacter cycloclastes has been purified to homogeneity under aerobic conditions via deae-cellulose, phenyl-sepharose, hydroxyapatite, and sephacryl s-200 chromatography. it consists of a single polypeptide of mw 72 kda, and contains 3.8 +/- 0.1 copper atoms per molecule. the enzyme is pink as isolated, yet exhibits a specific activity (86 u/mg) that is ca. 40 times greater than that observed for other n2o reductases under similar conditions. double integration o ... | 1990 | 2154217 |
| localization of the cytochrome cd1 and copper nitrite reductases in denitrifying bacteria. | the locations of cytochrome cd1 nitrite reductases in pseudomonas aeruginosa and pseudomonas fluorescens and copper nitrite reductases in achromobacter cycloclastes and achromobacter xylosoxidans were identified. immunogold labeling with colloidal-gold probes showed that the nitrite reductases were synthesized exclusively in anaerobically grown (denitrifying) cells. little immunogold label occurred in the cytoplasm of these four strains; most was found in the periplasmic space or was associated ... | 1990 | 2158973 |
| collagenase activation of latent matrix-degrading proteinases from human plasma fibronectin. | fibronectin contains two latent gelatinolytic enzymes, fn-gelatinase and fn-laminase that can be activated in the presence of ca2+ from the purified cathepsin d-produced 190-kda fibronectin fragment. the results of this work show that achromobacter collagenase cleaves fibronectin and generates an active fn-gelatinase. in contrast to the cathepsin d digest, the collagenase digest directly exhibits gelatinolytic activity without additional activation. the gelatinolytic activity of the total collag ... | 1990 | 2159310 |
| spin-equilibrium and heme-ligand alteration in a high-potential monoheme cytochrome (cytochrome c554) from achromobacter cycloclastes, a denitrifying organism. | a c-type monoheme cytochrome c554 (13 kda) was isolated from cells of achromobacter cycloclastes iam 1013 grown anaerobically as a denitrifier. the visible absorption spectrum indicates the presence of a band at 695 nm characteristic of heme-methionine coordination (low-spin form) coexisting with a minor high-spin form as revealed by the contribution at 630 nm. magnetic susceptibility measurements support the existence of a small contribution of a high-spin form at all ph values, attaining a min ... | 1990 | 2159881 |
| aspi, a novel isoschizomer of tth111i [corrected] from achromobacter species 699 recognizing 5'-gacn/nngtc-3'. | 1990 | 2162524 | |
| epr spectra of ferric cytochromes c' from five strains of achromobacter xylosoxidans at low temperature and their temperature dependence. | the epr spectra at low temperature (6 k) and their temperature dependence (10-93 k) for five ferric cytochromes c' isolated from chemoheterotrophic bacteria, achromobacter xylosoxidans ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, gifu 1048, gifu 1051, and gifu 1764 are reported. the epr spectral results indicate that the ground state of the heme iron(iii) of cytochromes c' from these chemoheterotrophic bacteria can appear to be in an admixed spin state which consists of predominan ... | 1990 | 2163634 |
| susceptibility of baboon aorta elastin to proteolysis. | elastin was purified from baboon aorta using achromobacter collagenase and its susceptibility to proteolysis by various enzymes was studied. human leukocyte elastase (hle) hydrolysed baboon aortic elastin 8 times faster than human cathepsin g. bovine chymotrypsin had virtually no activity against this substrate. the kinetic constants v and [s50] of aortic elastin hydrolysis by hle (0.15 microm) were 0.00286 mg x ml-1 x min-1 and 0.158 mg x ml-1, respectively. one mg of this elastin could be satu ... | 1990 | 2165785 |
| in vitro activity of pd127,391, a new quinolone against bacterial isolates from cancer patients. | the in vitro activity of pd127,391, a new 4-quinolone, was compared to that of ciprofloxacin against common clinical bacterial isolates from patients with cancer. pd127,391 was found to have a broad antimicrobial spectrum with excellent activity against gram-positive isolates (including multidrug-resistant organism such as corynebacterium jeikeium, enterococcus faecalis, enterococcus faecium, methicillin-resistant staphylococcus aureus and coagulase-negative staphylococcus spp.). it was also ext ... | 1990 | 2209169 |
| molecular cloning and nucleotide sequence of the beta-lytic protease gene from achromobacter lyticus. | two bacteriolytic enzymes secreted by achromobacter lyticus m497-1 were purified and identified as being very similar (considering their amino acid composition and n-terminal sequence) to alpha- and beta-lytic proteases from lysobacter enzymogenes. a 1.8-kb ecori fragment containing the structural gene for beta-lytic protease was cloned from a. lyticus chromosomal dna. the protein sequence deduced from the nucleotide sequence was identical to the known sequence of beta-lytic protease, except for ... | 1990 | 2228973 |
| complete amino acid sequence of rat kidney ornithine aminotransferase: identity with liver ornithine aminotransferase. | the complete amino acid sequence of rat kidney ornithine aminotransferase [ec 2.6.1.13] is presented. the 404-residue sequence was determined by analysis of peptides generated by digestion of the s-carboxyamidomethylated protein with cnbr, achromobacter protease i, arginylendopeptidase, or staphylococcus aureus v8 protease. mueckler and pitot have reported the amino acid sequence of the rat liver enzyme (440 residues) as predicted from the nucleotide sequence of the cdna [mueckler, m.m. & pitot, ... | 1990 | 2229004 |
| amino acid sequence of a lectin from japanese frog (rana japonica) eggs. | the complete amino acid sequence and the location of disulfide bonds of a lectin from japanese frog (rana japonica) eggs, which specifically agglutinates transformed cells, are presented. the sequence was determined by analysis of peptides generated by digestion of the s-carboxyamidomethylated protein with achromobacter protease i, or chymotrypsin, and by chemical cleavage with bnps-skatole or cyanogen bromide. the lectin is a single-chain protein consisting of 111 residues, with a pyroglutamyl ... | 1990 | 2229005 |
| achromobacter xylosoxidans bacteremia. | 1990 | 2230043 | |
| achromobacter xylosoxidans: a drug-resistant pathogen in newborns and immunocompromised patients. | 1990 | 2237562 | |
| differentiation of achromobacter-like strains from human blood by dna restriction endonuclease digest and ribosomal rna gene probe patterns. | variation amongst achromobacter-like strains was examined by dna restriction endonuclease digestion and rdna gene patterns generated using a non-radioactive probe. chromosomal dna was extracted from 12 cultures representing achromobacter groups b, e and f, all from human blood cultures. dna fingerprinting using ecori, hae iii or hindiii sub-divided the strains in a similar manner to that obtained by their protein patterns. the haeiii patterns, with their small number of bands, were the easiest t ... | 1990 | 2249718 |
| the complete amino acid sequence of the mature form of rat sepiapterin reductase. | the partial amino acid sequence of rat sepiapterin reductase was determined using peptides generated by cleavage of the s-carboxyamidomethylated protein with achromobacter protease i, cyanogen bromide, chymotrypsin or bnps-skatole. the protein began with n-acetyl methionyl residue at the n-terminus and ended with isoleucyl residue at the c-terminus. the present results essentially coincided with the amino acid sequence predicted from the nucleotide sequence of the cdna recently reported by citro ... | 1990 | 2260974 |
| [site-specific endonucleases lpli and aagi]. | new site-specific endonucleases lpli and aagi have been isolated from the lactobacillus plantarum and achromobacter agile cells, respectively. the enzymes' purification stages included treatment of cell-free extracts with polyethylenimine, fractionation in two-phase system by albertsson's method, chromatography on blue sepharose and deae-cellulose. the results of cleavage of a 5'-32p-labelled oligodeoxynucleotide duplex by restriction endonucleases lpli and aagi indicate that these enzymes recog ... | 1990 | 2285421 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. reported by the research group for testing imipenem susceptibility on clinical isolates]. | this study was conducted to investigate susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antibacterial agents at 64 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were carried out at each laboratory and the tests were performed according to the disk dilution method recommended by nccls in which susceptibilities are classified into "s", "ms", "i" and "r". ipm showed markedly high in vit ... | 1990 | 2287060 |
| purification and complex formation analysis of a cysteine proteinase inhibitor (cystatin) from seeds of wisteria floribunda. | seeds of wisteria floribunda contain several kinds of cysteine proteinase inhibitor (cystatin). we purified and characterized one of these inhibitors, named wcpi-3. the molecular weight of wcpi-3 was estimated to be 17,500 and 15,700 by gel filtration and sds-page, respectively. the isoelectric point was 5.7. wcpi-3 formed an equimolar complex with native papain and the dissociation constant was estimated to be 6.1 nm. complex formation between wcpi-3 and cys25-modified papain, such as s-carboxy ... | 1990 | 2292589 |
| microbial keratitis and corneal ulceration associated with therapeutic soft contact lenses. | we reviewed the records of 22 patients whose corneal ulcers were associated with therapeutic soft contact lens wear. the patients required hospitalization on the cornea service at wills eye hospital between january 1, 1978 and september 1, 1988. a majority of the ulcers were associated with pseudophakic or aphakic bullous keratopathy (9 of 22 cases; 41%); neurotrophic/exposure keratitis was the second most common diagnosis (7 of 22; 32%). most patients used topical antibiotics (15 of 22; 68%) an ... | 1990 | 2306853 |
| catheter-related right-sided endocarditis in bone marrow transplant recipients. | bone marrow transplant recipients are at increased risk of severe central venous catheter-related septicemias that may be complicated by endocardial infection. in view of this, we prospectively evaluated 141 consecutive patients receiving allogeneic or autologous bone marrow infusion. seven (5%) of 141 patients developed eight episodes of a clinical syndrome compatible with catheter-related right-sided infective endocarditis; this diagnosis was confirmed at autopsy in two patients who died. stap ... | 1990 | 2330480 |
| purification and amino acid sequence of basic protein i, a lysine-49-phospholipase a2 with low activity, from the venom of trimeresurus flavoviridis (habu snake). | a basic protein (pi 10.2), named basic protein i, was purified to homogeneity from the venom of trimeresurus flavoviridis (habu snake) after four chromatographic steps. the amino acid sequence of this protein was determined by sequencing the s-pyridylethylated derivative of the protein and its peptides produced by chemical (cyanogen bromide and formic acid) and enzymatic (chymotrypsin, achromobacter protease i, and staphylococcus aureus v8 protease) cleavages. the protein consisted of 122 amino ... | 1990 | 2330604 |
| achromobacter group b replacement valve endocarditis. | 1990 | 2341737 | |
| a distinct human testis and brain mu-class glutathione s-transferase. molecular cloning and characterization of a form present even in individuals lacking hepatic type mu isoenzymes. | mu-class glutathione s-transferases (gsts) were identified in all 13 human testes and 28 brains examined; even subjects whose livers were devoid of mu-gsts expressed extrahepatic gsts of this class. testes and brains from individuals with mu-class gsts in their livers had additional forms that also reflected the liver phenotypes. an isoenzyme with an isoelectric point of 5.2, which was a major gst in testis and present as well in cerebral cortex but not detected in any livers, was identified and ... | 1990 | 2345169 |
| rapid purification and characterization of human platelet glycoprotein v: the amino acid sequence contains leucine-rich repetitive modules as in glycoprotein ib. | glycoprotein v (gpv) is a membrane-associated, 82 kd platelet glycoprotein that is hydrolyzed during thrombin activation to yield 69 kd fragment. we have developed a rapid and simple method for isolation of the protein from platelet extracts using a combination of gel permeation, anion-exchange, and lectin affinity chromatography. the partial amino acid sequence was determined by analysis of peptides generated by digestion of the s-carboxyamido-methylated protein with achromobacter protease i or ... | 1990 | 2350580 |
| purification, amino acid sequence, and some properties of rabbit kidney lysozyme. | the lysozyme (rabbit kidney lysozyme) from the homogenate of rabbit kidney (japanese white) was purified by repeated cation-exchange chromatography on bio-rex 70. the amino acid sequence was determined by automated gas-phase edman degradation of the peptides obtained from the digestion of reduced and s-carboxymethylated rabbit lysozyme with achromobacter protease i (lysyl endopeptidase). the sequence thus determined was kiyercelartlkklgldgykgvslanwmclakwessyntratnynpgdkstdygifq insrywcndgktpravn ... | 1990 | 2361954 |
| structure of recombinant human interleukin 5 produced by chinese hamster ovary cells. | the complete peptide map of purified recombinant human interleukin 5 (rhil-5) was determined to verify its primary structure, glycosylation sites, and disulfide bonding structure. each peptide fragment generated by achromobacter protease i (api) digestion was purified and characterized by amino acid analysis and amino acid sequence analysis. after digestion with api, we could identify all the peptides which were expected from human il-5 cdna sequence. the analyses of sulfhydryl content in rhil-5 ... | 1990 | 2361960 |
| numerical analysis of sodium dodecyl sulphate-polyacrylamide gel electrophoretic protein patterns for the classification, identification and typing of medically important bacteria. | a numerical analysis of one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoretic protein patterns of three separate bacterial groups is described. similarity between patterns was estimated using a correlation coefficient and is represented diagramatically by the unweighted pair-group with arithmetic averages method of clustering. protein patterns were scanned using a laser densitometer interfaced directly to a microcomputer, where calculations were performed semi-automatically ... | 1990 | 2364925 |
| steady-state nitric oxide concentrations during denitrification. | three species of denitrifying bacteria, paracoccus denitrificans, pseudomonas stutzeri strain jm300, and achromobacter cycloclastes, were allowed to reduce nitrate or nitrite in anaerobic, closed vials while the equilibration of gases between aqueous and gas phases was facilitated by vigorous stirring. the gas phase was sampled and analyzed for no with use of a chemiluminescence detector calibrated against bottled no standards or against no produced by the nitrite-iodide reaction. [noaq] was inf ... | 1990 | 2365685 |
| numerical analysis of electrophoretic protein patterns of 'achromobacter' group b, e and f strains from human blood. | thirty-two clinical strains representing 'achromobacter' groups b, e and f were characterized by one-dimensional sds-page of cellular proteins. all the strains were isolated from blood samples from hospital patients in the united kingdom. the protein patterns, which contained 40 to 45 discrete bands, were highly reproducible and were used as the basis for a numerical analysis which included all the protein bands. the 32 'achromobacter' strains formed two clusters at the 77% s level. the first, p ... | 1990 | 2370235 |
| phosphorylation of the virg protein of agrobacterium tumefaciens by the autophosphorylated vira protein: essential role in biological activity of virg. | agrobacterium tumefaciens virulence genes are induced by plant signals through the vira-virg two-component regulatory system. the vira protein is a membrane-spanning sensor molecule that possesses an autophosphorylating activity, and the virg protein is a sequence-specific dna-binding protein. in this report, we demonstrate that the virg protein is phosphorylated by the vira protein and that the phosphate is directly transferred from the phosphorylated vira molecule (phosphohistidine) to the vir ... | 1990 | 2394678 |
| detection and identification of groundwater bacteria capable of escaping entrapment on 0.45-micron-pore-size membrane filters. | rural drinking water systems supplied by untreated groundwater were examined to determine whether coliform or heterotrophic plate count bacteria are capable of escaping entrapment on standard porosity (0.45-micron-pore-size) membrane filters. filterable bacteria were present in 42% of the 24 groundwater sources examined by using nonselective media (r2a, full strength m-hpc, and 0.1x m-hpc agars). pseudomonads were the most frequently identified group of filterable bacteria detected. flavobacteri ... | 1991 | 1768096 |
| purification and some properties of phospholipase c from achromobacter xylosoxidans. | a non-haemolytic phospholipase c (ec 3.1.4.3) was purified from the culture medium of achromobacter xylosoxidans with a 5% yield and a purification factor of 330. a combination of ultrafiltration, acetone precipitation and two subsequent affinity chromatographic steps was used. the affinity chromatography is a new application of 2-(4-aminophenylsulphonyl)ethyl-cellulose, a sorbent that has previously been used for the purification of phospholipase c from bacillus cereus. the purified enzyme gave ... | 1991 | 1783637 |
| identification of lysyl residues at the amp-binding site of biodegradative threonine deaminase from escherichia coli. | the biodegradative threonine deaminase from escherichia coli is activated allosterically by amp. to identify the residues interacting with the phosphate group of amp at the binding site, we used the affinity labeling reagent, adenosine diphosphopyridoxal (ap2-pl). in the absence of amp, the enzyme formed the schiff base with ap2-pl and scatchard plot analysis showed a biphasic pattern, the respective kd values for the high- and low-affinity binding phases being 20 and 110 microm. the former valu ... | 1991 | 1794987 |
| [susceptibilities of clinical bacterial isolates to antimicrobial agents, 1989. a study mainly focused on imipenem. the research group for testing imipenem susceptibilities of clinical isolates]. | we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at hospital laboratories throughout japan from september to december of 1989. the susceptibility testing was carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: (+++), (++), (+) and (-). ipm showed markedly high in vitro activities against streptococcus pneumoniae, neisseria gonorrhoeae, moraxella catarrhalis, es ... | 1991 | 1798067 |
| beta-d-glucuronidase (bdg) activity of gram-negative bacteria. | bdg is an inducible enzyme that is encoded by the uida gene in escherichia coli. genetic sequences of this gene are present in most if not all e. coli strains regardless of the bdg phenotype. expression of bdg activity can be influenced by lactose-induced catabolite repression or genetic mutations. salmonella, shigella and yersinia strains frequently exhibit positive bdg reaction. bdg activity of strains belonging to genus edwardsiella, serratia, yersinia, vibrio, erwinia, alcaligenes, acinetoba ... | 1991 | 1817425 |
| [achromobacter xylosoxidans septicemia]. | 1991 | 1827195 | |
| [an uncommon germ of meningitis, achromobacter xylosoxidans]. | 1991 | 1829828 | |
| amino acid sequence of nitrite reductase: a copper protein from achromobacter cycloclastes. | the amino acid sequence of the copper-containing nitrite reductase (ec 1.7.99.3) from achromobacter cycloclastes strain iam 1013 has been determined by using peptides derived from digestion with achromobacter protease i (lys), staphylococcus aureus v8 protease (glu), cyanogen bromide, and bnps-skatole in acetic acid. the subunit contains 340 amino acids. the identity of the first seven amino acids is tentative. the sequence has been instrumental in the x-ray structure determination of this molec ... | 1991 | 1830217 |
| [the dna nucleotide composition of bacteria oxidizing diethylene glycols]. | nucleotide composition of dna has been analyzed in bacteria composing the diethylene glycol-oxidizing association. it is shown that the microorganisms under study are representatives of genera alkaligenes and achromobacter. | 1991 | 1861652 |
| the 2.3 angstrom x-ray structure of nitrite reductase from achromobacter cycloclastes. | the three-dimensional crystal structure of the copper-containing nitrite reductase (nir) from achromobacter cycloclastes has been determined to 2.3 angstrom (a) resolution by isomorphous replacement. the monomer has two greek key beta-barrel domains similar to that of plastocyanin and contains two copper sites. the enzyme is a trimer both in the crystal and in solution. the two copper atoms in the monomer comprise one type i copper site (cu-i; two his, one cys, and one met ligands) and one putat ... | 1991 | 1862344 |
| achromobacter xylosoxidans. an unusual neonatal pathogen. | perinatal acquisition of a rare pediatric pathogen, achromobacter xylosoxidans, with evidence for in utero transmission, is described. cultures from the mother and neonate demonstrated a. xylosoxidans. an ascending bacterial infection in the mother with clinical chorioamnionitis is presented as the probable mode of transmission. postmortem examination of the infant confirmed achromobacter meningitis. in contrast to the current case with transmission from mother to neonate, previously published n ... | 1991 | 1862776 |
| [sepsis caused by achromobacter xylosoxidans]. | 1991 | 1863629 | |
| cysteine-86 is not needed for the enzymic activity of glutathione s-transferase 3-3. | recombinant glutathione s-transferase 3-3 expressed in spodoptera frugiperda (sf9) cells with the use of a baculovirus expression system was modified with 1 mm-iodoacetamide. amino acid analysis indicated that 0.79 +/- 0.15 cysteine residue was modified per enzyme subunit. the s-carbaminomethylated protein retains the gsh-conjugating activity. glutathione s-transferase 3-3 modified with iodo[14c]acetamide was digested with achromobacter proteinase i and the resulting peptides were separated by h ... | 1991 | 1883338 |
| rapid purification and characterization of homoserine dehydrogenase from saccharomyces cerevisiae. | homoserine dehydrogenase of saccharomyces cerevisiae has been rapidly purified to homogeneity by heat and acid treatments, ammonium sulfate fractionation, and chromatography on matrex gel red a and q-sepharose columns. the final preparation migrated as a single entity upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a mr of 40,000. the mr of the native enzyme was 81,000 as determined by gel filtration, suggesting that the enzyme is composed of two identical subunits. this feat ... | 1991 | 1897932 |
| role of the c-terminal region of smg p25a in its interaction with membranes and the gdp/gtp exchange protein. | smg p25a is a ras p21-like small gtp-binding protein which is implicated in the regulated secretory processes. we have recently found that bovine brain smg p25a is geranylgeranylated at its c-terminal region. in this study, we examined the function(s) of the c-terminal region of smg p25a. limited proteolysis of bovine brain smg p25a with achromobacter protease i produced an n-terminal fragment and a c-terminal tail. the mrs of intact smg p25a, the n-terminal fragment, and the c-terminal tail wer ... | 1991 | 1899908 |
| a novel lipopolysaccharide-binding hemagglutinin isolated from hemocytes of the solitary ascidian, halocynthia roretzi: it can agglutinate bacteria. | a hemagglutinin was isolated from hemocytes of the ascidian, halocynthia roretzi, by a procedure including extraction and ion-exchange chromatography on cm-cellulose. the molecular weight of the hemagglutinin was estimated to be 120,000 by gel filtration. it was resistant to acid treatment but sensitive to alkali or heat treatment. the hemagglutinating activity was inhibited by heparin, chondroitin sulfate, and lipopolysaccharide (lps), but not by mono- and disaccharides such as n-acetyl-galacto ... | 1991 | 1904830 |
| the posttranslationally modified c-terminal structure of bovine aortic smooth muscle rhoa p21. | rhoa p21, a ras p21-like small gtp-binding protein, has the same c-terminal consensus motif of cys-a-a-x (a is an aliphatic amino acid and x is any amino acid) as ras p21s, which is posttranslationally processed. we here determine the posttranslationally processed c-terminal structure of the rhoa p21 purified from bovine aortic smooth muscle. incubation of rhoa p21-expressing insect cells with exogenous [3h]mevalonolactone caused the labeling of rhoa p21, suggesting that rhoa p21 is prenylated. ... | 1991 | 1905729 |
| h218o isotope exchange studies on the mechanism of reduction of nitric oxide and nitrite to nitrous oxide by denitrifying bacteria. evidence for an electrophilic nitrosyl during reduction of nitric oxide. | reduction of no and no2-by whole cells of eight strains of denitrifying bacteria known to contain either heme cd1 or copper-containing nitrite reductases (nirs) has been examined in the presence of h218o. all organisms containing heme cd1 nirs exhibited relatively large extents of exchange between no2- and h218o (39-100%), as monitored by the 18o content of product n2o. organisms containing copper nirs gave highly variable results, with achromobacter cycloclastes and pseudomonas aureofaciens exh ... | 1991 | 1906460 |
| primary structure of nuclease p1 from penicillium citrinum. | the primary structure of nuclease p1, which cleaves both rna and single-stranded dna, from penicillium citrinum was elucidated. the complete amino acid sequence consisting of 270 residues was determined by analysis of peptides obtained by digestion with achromobacter protease i of the reduced and s-aminoethylated protein and by digestion with staphylococcus aureus v8 protease of the reduced and s-carboxymethylated protein. four half-cystine residues were assigned to cys72-cys217 and cys80-cys85. ... | 1991 | 1915339 |
| bacteria associated with tegument of clinostomum marginatum (digenea). | adults of clinostomum marginatum freshly collected from a heron, ardea herodias, were examined using transmission electron microscopy. specimens from the mouth of the bird were encrusted with bacteria that were not removed by washing unless the saline contained antibiotics. there was no evidence that the attached bacteria were damaging to the trematode tegument. three species of gram-negative bacteria were isolated from the worm surfaces and identified; achromobacter sp. was present in pure cult ... | 1991 | 1919930 |
| complete amino acid sequence of human liver cytosolic alanine aminotransferase (gpt) determined by a combination of conventional and mass spectral methods. | the complete amino acid sequence of human liver cytosolic alanine aminotransferase (gpt) (ec 2.6.1.2) is presented. two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and achromobacter protease i, respectively. isolated peptides were analyzed with a protein sequencer or with a plasma desorption time of flight mass spectrometer and placed in the sequence on the basis of their molecular mass and hom ... | 1991 | 1931970 |
| evidence for a no-rebound mechanism for production of n2o from nitrite by the copper-containing nitrite reductase from achromobacter cycloclastes. | reduction of no2- by the cu-containing nitrite reductase from achromobacter cycloclastes produces no as the primary product initially, but as no accumulates, no production levels-off and n2o production becomes significant. reaction of the enzyme with no2- in the presence of no increases the amount of n2o product significantly, while trapping the no product as nitrosylhemoglobin or rapid removal of no by sparging results in no detectable n2o production. reaction of the enzyme with 15no2- in the p ... | 1991 | 1936249 |
| amino acid sequence of nuclease s1 from aspergillus oryzae. | the amino acid sequence of nuclease s1, a nuclease which cleaves both single-stranded dna and rna, from aspergillus oryzae was determined. reduced and s-carboxymethylated or s-aminoethylated nuclease s1 was digested with achromobacter protease i, staphylococcus aureus v8 protease, or endoproteinase asp-n. peptides thus obtained were purified by reverse-phase high-performance liquid chromatography and sequenced, and the complete primary structure was established. nuclease s1 consists of a single ... | 1991 | 1939022 |
| in vitro activity of chlorhexidine against enteric rods, pseudomonads and acinetobacter from human periodontitis. | fifty-eight periodontal isolates of the families enterobacteriaceae and pseudomonadaceae, and the genera acinetobacter and achromobacter were studied to determine their susceptibility to peridex (0.12% chlorhexidine digluconate solution; procter & gamble). in an agar dilution assay, about 50% of the study strains grew in the presence of 70 micrograms/ml of chlorhexidine. enterobacter cloacae, klebsiella pneumoniae, pseudomonas aeruginosa, pseudomonas cepacia, and serratia marcescens comprised th ... | 1991 | 1945483 |
| achromobacter xylosoxidans septic arthritis in a patient with systemic lupus erythematosus. | 1991 | 1953827 | |
| spectral properties of achromobacter xylosoxidans cytochromes c' and their no complexes. | cytochromes c' have been isolated from six strains of achromobacter xylosoxidans: ncib 11015 (formerly alcaligenes sp. ncib 11015), gifu 543, 1048, 1051, 1055 and 1764. they are dimeric proteins with more positive redox potentials than those of cytochromes c' from phototrophic bacteria at neutral ph. the electronic absorption, epr and mcd spectra on no-ferrous cytochromes c' at physiological ph showed that the major part of the heme-iron of nitrosylheme was penta-coordinated. the epr spectral re ... | 1991 | 1646026 |
| proton and tritium nmr relaxation studies of peptide inhibitor binding to bacterial collagenase: conformation and dynamics. | the interaction of succinyl-pro-ala, a competitive inhibitor of achromobacter iophagus collagenase, with the enzyme was studied by longitudinal proton and tritium relaxation. specific deuterium and tritium labeling of the succinyl part at vicinal positions allowed the measurement of the cross-relaxation rates of individual proton or tritium spin pairs in the inhibitor-enzyme complex as well as in the free inhibitor. overall correlation times, internuclear distances, and qualitative information o ... | 1991 | 1651124 |
| [structure and function of cytochrome c' from achromobacter xylosoxidans]. | 1991 | 1665239 | |
| achromobacter xylosoxidans infection in baboons. | 1991 | 1666159 | |
| a prion protein missense variant is integrated in kuru plaque cores in patients with gerstmann-sträussler syndrome. | kuru plaques are the pathologic hallmark in gerstmann-sträussler syndrome (gss). to demonstrate that prion protein (prp) is a component of kuru plaque cores, we fractionated and sequenced kuru plaque core derived peptides, following digestion with achromobacter lyticus protease i. we identified 3 prp-derived peptides by reverse-phase high-performance liquid chromatography and found a fragment of digests derived from a missense variant of prp. the variant prp was also present in the prion rod fra ... | 1991 | 1671530 |
| puncture wound-induced achromobacter xylosoxidans osteomyelitis of the foot. | 1991 | 1674638 | |
| p-cresol methylhydroxylase from a denitrifying bacterium involved in anaerobic degradation of p-cresol. | a bacterium, strain pc-07, previously isolated as part of a coculture capable of growing on p-cresol under anaerobic conditions with nitrate as the acceptor was identified as an achromobacter sp. the first enzyme of the pathway, p-cresol methylhydroxylase, which converts its substrate into p-hydroxybenzyl alcohol, was purified. the enzyme had an mr of 130,000 and the spectrum of a flavocytochrome. it was composed of flavoprotein subunits of mr 54,000 and cytochrome subunits of mr 12,500. the mid ... | 1991 | 1991722 |
| amino acid sequence and molecular characterization of a d-galactoside-specific lectin purified from sea urchin (anthocidaris crassispina) eggs. | the complete amino acid sequence of a 11.5-kda subunit of d-galactoside binding lectin purified from sea urchin (anthocidaris crassispina) eggs is presented. the 105-residue sequence of the subunit was determined by analysis of the intact s-carbamoylmethylated protein and peptides generated by digestion with achromobacter protease i or staphylococcus aureus v8 protease. the lectin exists as a disulfide-linked homodimer of two subunits; the dimeric form is essential for hemagglutination activity. ... | 1991 | 2001368 |
| human recombinant cuzn-superoxide dismutase. amino acid sequence and location of the disulfide bond. | the complete amino acid sequence of recombinant human cu-zn superoxide dismutase (cuznsod) is presented. the s-carboxymethylated protein was cleaved at lysine residues (with achromobacter protease i) to provide a set of nine non-overlapping fragments accounting for 90% of the sequence. these fragments were then overlapped and aligned, and the sequence was completed by using peptides generated by cleavage at glutamic acid residues (with s. aureus v8 protease) and at arginine (with clostripain). t ... | 1991 | 2019474 |
| catalysis of nitrosyl transfer by denitrifying bacteria is facilitated by nitric oxide. | two denitrifying bacteria, pseudomonas stutzeri and achromobacter cycloclastes, were incubated with na15no2 and nan3 under conditions that allowed catalysis of nitrosyl transfer from nitrite to azide. this transfer, which is presumed to be mediated by the heme- and copper-containing nitrite reductase of p. stutzeri and a. cycloclastes, respectively, leads to formation of isotopically mixed 14,15n2o, whereas denitrification leads to 15n2o. the conditions that emphasized nitrosyl transfer also par ... | 1991 | 2025262 |
| complete amino acid sequence of rat liver cytosolic alanine aminotransferase. | the complete amino acid sequence of rat liver cytosolic alanine aminotransferase (ec 2.6.1.2) is presented. two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and achromobacter protease i, respectively. the protein was found to be acetylated at the amino terminus and contained 495 amino acid residues. the molecular weight of the subunit was calculated to be 55,018 which was in good agreement with ... | 1991 | 2043642 |
| amino acid sequence of a phospholipase a2 from the venom of trimeresurus gramineus (green habu snake). | two phospholipases a2, named phospholipases a2-i and a2-ii, were purified to homogeneity from the venom of trimeresurus gramineus (green habu snake). the complete amino acid sequence of phospholipase a2-i was determined by sequencing the native protein and the peptides produced by enzymatic (achromobacter protease i, clostripain, and chymotrypsin) and chemical (hydroxylamine) cleavages of the s-pyridylethylated derivative of the protein. the protein consisted of 122 amino acid residues and was s ... | 1991 | 2048135 |
| a new procedure for enzymatic semisynthesis of human insulin by hydrolysis of single-chain des-(b-30)-lnsulin precursor with lysyl endopeptidase. | it has been shown that the single-chain des-(b-30)-insulin precursor (sci) can be converted into human insulin ester by transpeptidation using trypsin in the presence of a threonine derivative. the present study demonstrates that achromobacter lyticus protease 1 (lysyl endopeptidase) can catalyze the transpeptidation reaction more efficiently than can trypsin. it is also shown that des-(b-30)-insulin (dai) can be produced by hydrolysis of sci with the lysyl endopeptidase. since it is well known ... | 1991 | 18600661 |
| relationship between cell surface properties and transport of bacteria through soil. | a study was conducted to relate the properties of enterobacter, pseudomonas, bacillus, achromobacter, flavobacterium, and arthrobacter strains to their transport with water moving through soil. the bacteria differed markedly in their extent of transport; their hydrophobicity, as measured by adherence to n-octane and by hydrophobic-interaction chromatography; and their net surface electrostatic charge, as determined by electrostatic interaction chromatography and by measurements of the zeta poten ... | 1991 | 16348394 |
| inhibition of achromobacter protease i by lysinal derivatives. | z-val-, z-pro-, z-leu-leu-, and z-leu-pro-lysinals and bz-dl-lysinal were chemically synthesized and tested as novel inhibitors for achromobacter protease i (api), a lysine-specific serine protease. among the lysinal derivatives tested, z-val-lysinal was the most potent competitive inhibitor, its ki being estimated as 6.5 nm in an esterolytic assay with tos-lys-ome. in an amidolytic assay, z-leu-leu-lysinal was the most potent inhibitor and the apparent mode of inhibition was non-competitive. th ... | 1992 | 1369061 |
| construction of protein analogues by site-specific condensation of unprotected fragments. | the extreme sensitivity to periodate of 1-amino, 2-hydroxy compounds permits the selective conversion of n-terminal serine and threonine to an aldehydic group. we have used this reaction to construct analogues of human granulocyte colony stimulating factor (g-csf) by allowing such oxidized peptides to react with others that have had a hydrazide derivative attached to the c-terminus by reversed proteolysis. two recombinant analogues of g-csf were used as starting materials. both had only a single ... | 1992 | 1381617 |
| prosthetic knee infection due to achromobacter xylosoxidans. | achromobacter xylosoxidans is an aerobic gram negative organism that has been infrequently implicated in clinical infections in a variety of anatomical sites. we describe a case of a prosthetic knee infection due to achromobacter xylosoxidans in a patient with rheumatoid arthritis receiving high dose prednisone. | 1992 | 1404142 |