Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| oral hairy leukoplakia. | oral hairy leukoplakia was first described in homosexual men infected with the human immunodeficiency virus. it is thought to be caused by infection with both the epstein-barr virus and human papillomavirus. we report 59 cases of oral hairy leukoplakia. the disease was diagnosed in patients in all risk groups and was categorized in all classes of the walter reed classification without significant differences in prevalence. epstein-barr virus could be demonstrated in all tissue samples examined; ... | 1990 | 2153716 |
| human papillomavirus type 6b dna required for initiation but not maintenance of transformation of c127 mouse cells. | we describe the transformation of c127 mouse fibroblasts with human papillomavirus type 6b (hpv-6b) dna, which is associated primarily with benign tumors of the human genital tract. the major transformed phenotype of the hpv-6b-transfected cells lines, which had been g418 selected, pooled, and maintained without subsequent selection, was tumorigenicity in nude mice. we found that, unlike that reported for other hpvs or papovaviruses, the transformed phenotype was expressed after a delay, in whic ... | 1990 | 2154622 |
| anal cloacogenic and squamous carcinomas. comparative histologic analysis using in situ hybridization for human papillomavirus dna. | in order to evaluate the morphologic and possible etiologic distinctions between anal cloacogenic and squamous carcinomas, we performed histologic examination and in situ hybridization for human papillomavirus (hpv) dna on anal canal and anal verge carcinomas from 37 patients. twenty-one neoplasms were invasive or in situ squamous carcinomas, 14 were invasive cloacogenic carcinomas, and two were unclassified. in situ hybridization was positive for hpv types 16/18 in 12 cases and for types 6/11 i ... | 1990 | 2154141 |
| longitudinal study of human papillomavirus infection of the female urogenital tract by in situ hybridization. | the recurrence and/or persistence of human papillomavirus (hpv)-associated lesions constitute a well-recognized clinical problem; yet, few studies have demonstrated and typed the hpv within these recurrence lesions. a patient with a 17-year history of anogenital condylomata presented with an unusual bladder tumor that had histologic features of hpv infection. this prompted a longitudinal analysis of hpv dna in archival material from these urogenital lesions. colorimetric in situ hybridization wa ... | 1990 | 2154168 |
| detection of human papilloma virus dna in semen from patients with intrameatal penile warts. | 1990 | 2164493 | |
| the dna-binding domain of hpv-16 e2 protein interaction with the viral enhancer: protein-induced dna bending and role of the nonconserved core sequence in binding site affinity. | we expressed the carboxy-terminal portion of the e2 open reading frame (orf)-encoded protein of human papillomavirus type 16 (hpv-16) and purified it to near homogeneity. using dnase i footprinting techniques, we show that like the homologous protein from bovine papillomavirus type 1 (bpv-1), hpv-18, hpv-11, it binds dna at the enhancer consensus motif accn6ggt. base and phosphate backbone contact points were determined using methylation protection and interference and ethylation interference as ... | 1990 | 2154890 |
| thermus aquaticus dna polymerase-catalysed chain reaction for the detection of human papillomaviruses. | to delineate the conditions for the polymerase chain reaction (pcr) using primers specific for human papillomavirus (hpv) types 6b, 16 and 18, a number of important technical features were analysed. buffer, concentrations of magnesium, taq polymerase, primers and dna templates, annealing temperature, and extension time were studied by a combination of gel electrophoresis, southern and slot-blot hybridization. amplification of e6 gene fragments of hpv-16 and hpv-18 generated bands of 110 bp and 1 ... | 1990 | 2155246 |
| production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. | a monoclonal antibody was raised against the major capsid protein l1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the l1 protein, as a target for screening. this antibody, designated camvir-1, reacted with a 56 kilodalton protein in cells infected with l1-vaccinia virus, and the protein was present in a predominantly nuclear location. the antibody also detects the hpv-16 l1 antigen in formalin fixed, paraffin wax embedded biopsy specimens and on routine cerv ... | 1990 | 2166093 |
| natural cell-mediated cytotoxicity against various target cells in patients with epidermodysplasia verruciformis. | natural cell-mediated cytotoxicity of peripheral blood mononuclear cells was studied in eight patients with epidermodysplasia verruciformis induced by human papillomaviruses specific for epidermodysplasia verruciformis and in five patients with epidermodysplasia verruciformis-induced exclusively by human papillomavirus type 3. nine patients with various cutaneous warts and 25 age- and sex-matched healthy persons were control subjects. natural cell-mediated cytotoxicity against both k-562 erythro ... | 1990 | 2155952 |
| an alternate view of the management of cervical human papillomavirus infections. | 1990 | 2155956 | |
| transcription of the transforming genes of the oncogenic human papillomavirus-16 is stimulated by tumor promotors through ap1 binding sites. | the promoter p97 of human papillomavirus-16 (hpv-16) gives rise to transcripts that encode the principal transforming genes of the virus, e6 and e7. the activity of p97 is regulated by a cell-type-specific enhancer, as well as by glucocorticoids and progesterone. we show here, that in caski cells, which contain hpv-16 genomes, p97 is also inducible by phorbol esters. functional analysis of restriction fragments and oligonucleotides of the viral enhancer localizes two phorbol ester response eleme ... | 1990 | 2156229 |
| [incidence of human papillomavirus types 31, 33 and 35 in cervix intra-epithelial neoplasms]. | 206 patients with cervical intraepithelial neoplasia (cin) were examined at the department of gynaecology and obstetrics of the university of tübingen for genital infections with human papilloma viruses (hpv) using dna-hybridisation. 1. the hpv-type-groups 31/33/35 and 16/18 occurred with approx. the same frequency. 2. significantly more potentially reversible changes (cin i.-ii.) were associated with hpv types 31, 33 and 35 than with hpv-types 16 and 18, which were found more often in cin iii. ... | 1990 | 2156747 |
| cytologic diagnosis of human papillomavirus infection: spindled nuclei. | the classic cytologic criteria, koilocytotic atypia and dyskeratocytosis, fail to identify most cases with colposcopic and histologic evidence of cervical condylomata. the purpose of this study was to identify a novel cytologic diagnostic criterion, spindled nuclei, to predict the presence of human papillomavirus (hpv) infection of the cervical epithelium. review of the hospital records of 100 consecutive cases with spindled nuclei on papanicolaou smear identified 36 patients in whom a colposcop ... | 1990 | 2167194 |
| detection of human papillomavirus in reprocessed routine papanicolaou smears by dna hybridization. | dna dot blot hybridization was used for the detection of human papillomavirus (hpv) types 6, 11, 16, and 18 in reprocessed routinely collected papanicolaou smears. dna was extracted from the smears with alkaline lysis and applied onto a nitrocellulose filter. the specificity and sensitivity of the dot blot hybridization on reprocessed papanicolaou smears were confirmed by southern blot analysis of selected samples, using caski, siha cell lines as positive controls and hf 32 as negative controls. ... | 1990 | 2167195 |
| human papillomavirus infection of the uterine cervix analyzed by nonisotopic in situ hybridization. | some types of human papillomaviruses (hpvs) have been suggested to be strongly related to uterine cervical carcinoma. an attempt to detect these in formalin-fixed, paraffin-embedded sections was made by either immunohistochemical or by in situ hybridization. anticapsid protein of bovine papillomavirus antibody labeled with peroxidase was used for immunohistochemistry, and biotin was used instead of radioisotopes to label probes for in situ hybridization, which resulted in low background and a ra ... | 1990 | 2167347 |
| dna hybridisation of routinely processed tissue for detecting hpv dna in anal squamous cell carcinomas over 40 years. | to study possible changes in the incidence of human papillomavirus (hpv) associated anal squamous cell carcinomas (scc) a simple, rapid, and sensitive technique (alkaline hydrolysis) to permit dna hybridisation from formalin fixed, paraffin wax embedded tissue was developed. the sensitivity and specificity of the technique were established by comparison with southern blot analysis and in situ hybridisation on the same tissue specimens. ninety tissue specimens in a single analysis were examined u ... | 1990 | 2156915 |
| effect of the menstrual cycle on detection and typing of human papillomavirus in uterine cervical cells. | we observe fluctuations in human papillomavirus detection and variation in genotyping between sequential cervical cell specimens analyzed by filter in situ hybridization. furthermore, specimen adequacy for analysis varies. to determine whether these phenomena are correlated with menstrual cycle stage at the time of sampling, we analyzed cervical cell specimens from women with cervical intraepithelial neoplasia. specimens were categorized on the basis of a 28-day menstrual cycle and were analyzed ... | 1990 | 2158233 |
| detection of five different human papillomavirus types in cervical lesions by in situ hybridization. a study of 110 cases using sulfonated probes. | several findings suggest an etiologic relationship between genital tract squamous cell carcinoma and certain types of human papillomavirus (hpv). detection of these hpv types in cervical lesions considered as preneoplastic states (ie, cervical intraepithelial neoplasia or cin) is extremely important but difficult because the morphology of these states is highly heterogeneous and clinical course is rarely predictable. in situ hybridization (ish) is the only technique allowing correlation between ... | 1990 | 2168337 |
| prevalence of human papillomavirus types 11, 16 and 18 in cervical swabs. a study of 1362 pregnant women. | cervical smears from 1362 pregnant women were examined by filter in situ hybridisation for human papillomavirus (hpv) types 11, 16 and 18. 119 women (8.7%) had hpv-positive smears, hpv 16 being the most common type (61% of all infections). there was a correlation with age (r = 0.63, p = 0.004), the highest incidence found in women less than 22 years old with a decline after the age of 30. the incidence of cervical hpv infection was significantly higher (20.3%, p less than 0.01) in the subgroup o ... | 1990 | 2159427 |
| a major transcript of human papillomavirus type 16 in transformed nih 3t3 cells contains polycistronic mrna encoding e7, e5, and e1--e4 fusion gene. | we have cloned cdna of the major 1.8 kb mrna from hpv 16-transformed nih 3t3 cells (pm3t3). the entire nucleotide sequences of this cdna were determined and compared with prototype hpv 16 genomic dna sequences. the 5'-end of the cdna was flanked by approximately 300 bp of cellular sequences, and the 3'-end of the cdna sequences contained poly a residues following at nt 4230. hpv 16 sequences began at nt 124, downstream of a major viral p97 promoter, within the e6 open reading frame (orf). the fi ... | 1990 | 2161158 |
| influence of human papillomavirus on dna ploidy determination in genital condylomas. | it has been reported that deoxyribonuclease (dnase) treatment does not destroy viral dna, but it does digest native nuclear dna. to determine what effect, if any, papillomavirus infection has on dna ploidy values of genitourinary condylomas, dna was measured with and without dnase exposure in seven urethral condylomas, shown by prior in situ hybridization to contain abundant human papillomavirus types 6 and 11. normal human skin was used as a negative control. consecutive paraffin-embedded tissu ... | 1990 | 2161279 |
| human papillomavirus infection. | the human papillomavirus, well known as the etiologic agent of warts, has recently received much attention in the medical literature for its association with various cancers. this article discusses the virology, epidemiology, pathogenesis, immunology, clinical manifestations, and therapy for human papillomavirus infection. some newer techniques for identifying human papillomavirus in tissue, based on recent advances in molecular biology, are also covered. two final topics addressed are human pap ... | 1990 | 2156916 |
| absence of human papillomavirus (hpv) dna sequences in epithelial ovarian carcinoma. | 1990 | 2157639 | |
| detection of human papillomavirus in skin and genital lesions of renal allograft recipients by in situ hybridization. | renal allograft recipients have an increased incidence of malignancy including squamous carcinoma of cervix and skin. there is growing evidence that human papillomavirus (hpv) has a part to play in malignant transformation at these sites. we have previously identified hpv dna in the skin and genital lesions of such patients by dot and southern blotting. in situ hybridization studies, using biotinylated dna probes for hpv 4, 5 and 8 in skin lesions and 6, 11, 16 and 18 in genital lesions, were pe ... | 1990 | 2157656 |
| detection of hpv 6 and 11 in tumours of the upper respiratory tract using the polymerase chain reaction. | we have applied the polymerase chain-reaction (pcr) technique to benign and malignant squamous tumours of the pharynx and larynx and to nasal inverted papillomas to detect evidence of infection with human papillomavirus (hpv) 6 and 11. each of these lesions tended to be infected with either or both of these but the prevalence of infection when compared with that of histologically normal biopsies from the nasopharynx was not significantly increased. | 1990 | 2161718 |
| human papillomavirus genital tract infections during pregnancy. | 1990 | 2161719 | |
| genome organization and taxonomic position of human papillomavirus type 47 inferred from its dna sequence. | the complete nucleotide sequence of human papillomavirus type 47 (hpv-47) dna isolated from the lesion of epidermodysplasia verruciformis (ev) was determined. the computer-aided comparison of hpv-47 with other ev-associated viruses using the available sequence data on them revealed that hpv-47 resembles both hpv-5 and hpv-8 as much as hpv-5 and hpv-8 resemble each other, and it led us to regard these three viruses as one cluster and hpv-19 and hpv-25 as another. the conclusion implies that hpv-4 ... | 1990 | 2162112 |
| detection of human papillomavirus-genomic dna in oral epithelial dysplasias, oral smokeless tobacco-associated leukoplakias, and epithelial malignancies. | human papillomavirus (hpv) is an infectious agent that is increasingly associated with mucosal cancers, in particular cancer of the cervix. the present investigation was undertaken in an attempt to determine whether hpv could be easily detected in biopsies of oral tissues, specifically oral squamous cell carcinomas, oral epithelial dysplasias, smokeless tobacco keratoses, verrucous hyperplasia, and verrucous carcinoma. in situ dna hybridization methods were used to isolate specific hpv genomes. ... | 1990 | 2170606 |
| antigen presentation and t-cell activation in epidermodysplasia verruciformis. | aberrant immune responses may play a role in the susceptibility of patients with epidermodysplasia verruciformis to human papilloma virus (hpv). we examined the stimulatory capacity of antigen-presenting cells from hpv-infected skin and peripheral blood t-cell responses of patients with epidermodysplasia verruciformis. the percentage of langerhans cells in relation to total epidermal cells in suspension was slightly reduced in hpv-infected lesions, relative to apparently clinically uninfected ep ... | 1990 | 2162366 |
| fast solid support detection of pcr amplified viral dna sequences using radioiodinated or hapten labelled primers. | oligonucleotides with novel modifications have been synthesized and incorporated into enzymatically amplified dna sequences. they allow the fast detection of viral dna sequences after two rounds of amplification. the hybrids formed are immobilized by affinity on coated tubes and detected by direct beta (32p) or gamma (125i) counting or by colorimetric revelation. the effect of a dilution step between the two amplifications is studied to obtain optimal sensitivity and specificity. this test is us ... | 1990 | 2162518 |
| lichen sclerosus, invasive squamous cell carcinoma, and human papillomavirus. | 1990 | 2163194 | |
| carbon dioxide laser energy disperses human papillomavirus deoxyribonucleic acid onto treatment fields. | the possibility of dispersing viral deoxyribonucleic acid during carbon dioxide laser treatment of human papillomavirus-containing genital infections has been investigated with a commercially available dot blot hybridization technique. the viral ribonucleic acid probes were specific for groups of human papillomavirus types 6/11, 16/18, and 31/33/35. laser energy was delivered by continuous-wave mode and the plume of smoke was evacuated by a vacuum suction system. samples were taken with dacron s ... | 1990 | 2171340 |
| vaginal condylomata acuminata after mcindoe neovagina creation. | the author encountered vaginal condylomata acuminata in two women after mcindoe neovagina creations. by in situ dna hybridization, the authors identified human papillomavirus type 6 in both women. the large exophytic pattern of growth in the neovagina was more characteristic of that encountered on the natural keratinized squamous epithelium of the vulva and perianal area than of the micropapillary and small flat lesions usually found on natural vaginal mucosa. these observations suggest that (1) ... | 1990 | 2163112 |
| the presence of human papillomavirus (hpv) in solitary adult laryngeal papillomas demonstrated by in-situ dna hybridization with sulphonated probes. | human papilloma virus (hpv) types 6 and 11 have been repeatedly demonstrated in multiple laryngeal papillomas, and there is little doubt that these lesions are caused by hpv. it has been clearly demonstrated in recent reports that the clinical course of solitary adult onset laryngeal papillomas is entirely different from that of multiple papillomas of juvenile as well as of adult onset. we here report the presence of hpv types 6 and 11 in 19 out of 20 solitary papillomas from 16 patients, while ... | 1990 | 2171818 |
| use of polymerase chain reaction for successful identification of asymptomatic genital infection with herpes simplex virus in pregnant women at delivery. | the polymerase chain reaction was adapted to the amplification of a herpes simplex virus (hsv) dna sequence, common to hsv types 1 and 2 (hsv-1, hsv-2). the amplified product was detectable by ethidium-bromide staining or southern hybridization of gels and by dot hybridization. the hsv polymerase chain reaction detected hsv dna in samples obtained from eight patients with genital lesions from which hsv-2 was isolated in tissue culture and from four patients with labial lesions from which hsv-1 w ... | 1990 | 2172392 |
| presence of hpv 16 sequences in laryngeal carcinomas. | human papillomavirus types hpv 16 and hpv 11 dna sequences were analyzed in normal and neoplastic tissues of the larynx, using the technique of polymerase chain reaction (pcr). an amplified region of e6 orf was hybridized with 3' end-labelled oligonucleotide probe. twenty six out of 48 (54%) squamous-cell carcinomas, and 3 out of 3 verrucous-cell carcinomas hybridized with hpv 16 dna sequences, whereas we did not detect hpv 11 sequences. hpv 16 dna sequences were also found in normal, autologous ... | 1990 | 2163992 |
| enhancing cervical cancer detection using nucleic acid hybridization and acetic acid tests. | acetowhitening of abnormal cervical epithelium has been suggested as an indicator of increased cervical cancer risk. the presence of human papillomavirus (hpv) types 16, 18, 31, 33 and 35 may also indicate increased cervical cancer risk. hence, tests that detect these two abnormal conditions may augment that papanicolaou smear (pap test) as predictors of cervical cancer risk. the cohort consisted of 145 women aged 14 to 47 (mean 21 years) attending health clinics. thirty women (20.6 percent) sho ... | 1990 | 2164177 |
| single application treatment of human papillomavirus infection of the cervix and vagina with trichloroacetic acid: a randomized trial. | treatment of human papillomavirus (hpv) infection of the lower genital tract with trichloroacetic acid was evaluated in a randomized, double-blind fashion at the hospital of the university of pennsylvania. thirty-four patients who had colposcopic and histologic evidence of hpv without dysplasia were entered into the study and randomly placed into either a treatment or placebo group. the treatment group received a single topical application of 50% trichloroacetic acid to the vagina and cervix on ... | 1990 | 2164652 |
| detection of cervical human papillomavirus by molecular probes. | 1990 | 2164110 | |
| [detection of papillomavirus common antigens in plantar epidermoid cysts]. | a total of 23 cases of human papillomavirus-associated epidermoid cysts, including one presented here, have been reported in japan. a review of the literature revealed that the appearance of eosinophilic intracellular substances and that of vacuolar substances in the horny layer of cysts characterize these cases. high detection of human papillomavirus, the pathological features in these cases, and the observation of our case from the onset in which verrucous lesions had preceded a cyst may favor ... | 1990 | 2165193 |
| teaching colposcopy and androscopy in family practice residencies. | addressing the widespread human papillomavirus and genital epithelial dysplasia epidemic requires mastery of colposcopy, androscopy, and cryotherapy. implementing a family medicine residency training program for these skills requires identifying a faculty facilitator to consider the issues of time, cost, caseload, reimbursement, specialist support, personal training, and office impact related to this training. experience with teaching these skills in a community-based family practice residency i ... | 1990 | 2166129 |
| failure to phosphorylate the retinoblastoma gene product in senescent human fibroblasts. | heterokaryon studies suggest that senescent and quiescent human diploid fibroblasts (hdf) contain a common inhibitor of entry into s phase. dna synthesis can be induced in senescent and quiescent hdf by fusing them with cells containing dna viral oncogenes such as sv40 t antigen, adenovirus e1a, or human papillomavirus e7. both senescent and quiescent hdf contained the unphosphorylated form (p110rb) of the retinoblastoma protein, a putative inhibitor of proliferation. after serum stimulation, se ... | 1990 | 2166342 |
| [asymptomatic and subclinical human papillomavirus infections of the genital organs and anus in males]. | 1990 | 2173853 | |
| detection of human papillomavirus dna in genital condylomata in women and their male partners by using in situ hybridization with digoxygenin labeled probes. | twelve couples (12 women and their male partners) presenting genital warts were investigated in order to evaluate the sexual transmission of human papillomavirus (hpv) in mutual partners and the localization of hpv dna. formalin-fixed, paraffin-embedded biopsy samples of 12 vulvar condylomata, and 12 penile condylomata from male partners were analyzed for the presence of hpv dna-6, -11, and 16/18 by using in situ hybridization with digoxygenin labeled dna probes. hpv dna was identified in 9 wome ... | 1990 | 2166362 |
| detection of human papilloma virus in women referred for colposcopy. a comparison between different diagnostic methods. | various methods presently available for the diagnosis of genital human papilloma virus (hpv) were compared regarding their sensitivity in women referred for specific diagnosis and treatment because of atypical pap smears or clinically suspected neoplasia. colposcopic examination was performed in all cases. in addition to taking a second pap smear, cell suspensions were made from 105 women and analysed by the filter in situ hybridization (fish) technique and tested for hpv 6 + 11 and hpv 16 + 18 ... | 1990 | 2166988 |
| ubiquitous and cell-type-specific protein interactions with human papillomavirus type 16 and type 18 enhancers. | we have studied the protein-dna interactions of human papillomavirus types 16 and 18 constitutive enhancer elements using dnasel footprinting experiments with nuclear extracts from four cervical carcinoma cell lines (c33a, hela, siha, and caski) and one fibroblast cell line (143b). among nine footprints for the hpv 16 enhancer region, six footprints contain nuclear factor 1 (nf1) binding gccaa motif. in vitro competition experiments suggest that the same factors are shared by all six of these mo ... | 1990 | 2167563 |
| genitoanal papillomavirus infection--a diagnostic and therapeutic dilemma. | there are now known to be many genotypes of human papillomavirus (hpv). types 6 and 11 are closely associated with benign lesions of genitoanal epithelia; the classical forms are condylomata acuminata and papular warts, collectively known as genitoanal or "venereal" warts, but it is now known that subclinical infections are much more common. these were first identified on the cervix uteri by colposcopy after the application of acetic acid, but they can also be found on penile and vulval epitheli ... | 1990 | 2167722 |
| [diagnosis and follow-up study of human papillomavirus infection in benign epithelium and mild dysplasia of the uterine cervix]. | in this study, we evaluated the sensitivity of cytology, colposcopy and the dot blot method (vira pap, vira type) for the screening of hpv infection in benign epithelium and mild dysplasia of the uterine cervix, and then investigated subsequent changes in hpv dna in the lesion. the hpv infection detection rates for cytology, colposcopy and the dot blot method were 14/46 (30.4%), 30/46 (65.2%) and 35/46 (76.1%), respectively. cases where hpv dna was negative with the dot blot method but hpv infec ... | 1990 | 2169511 |
| biotin and phosphorus-isotopic labelled dna/rna probes for the detection of human papilloma virus sequences. | in this study, the diagnostic accuracy and practicability of different hybridization techniques for the detection of human papilloma virus (hpv) dna were tested. cervical cell scrapes (n = 67) were analysed for hpv-dnas 6/11 and 16, in order to compare a commercially available in situ dna hybridization-assay with the conventional southern-blot analysis. the in situ dna hybridization-assay gave a sensitivity of 81.5%, a specificity of 97.5% and a diagnostic efficiency of 91.0% for hpv-dnas 6/11. ... | 1990 | 2169684 |
| condylomata acuminata in children: frequent association with human papillomaviruses responsible for cutaneous warts. | to identify the papillomavirus types associated with condylomata acuminata in children and to evaluate their mode of transmission, we studied 32 children with anogenital warts. external condylomata were found in 12 of their mothers and in 10 of their fathers. ten mothers, including two without external lesions, had cervical condylomata. blot hybridization studies disclosed a genital human papillomavirus (hpv) in 14 of 27 children (hpv-6 in 12 and hpv-11 in two) and in 8 of 14 patients (hpv-6 in ... | 1990 | 2170467 |
| langerhans cells and epithelial cell modifications in cervical intraepithelial neoplasia: correlation with human papillomavirus infection. | the study of a series of 18 cervical intraepithelial neoplasia (cin) grade ii and iii was aimed at determining the distribution and phenotype of immunocompetent cells (langerhans cells, t and nk cells) and the alteration in the expression of egf receptors and beta 2-microglobulin in correlation with human papillomavirus (hpv) infection (viral antigen and dna typing with biotinylated probes). these lesions were characterized by a reduced number of langerhans cells and a dense infiltrate. hpv infe ... | 1990 | 2168858 |
| fast multiplex polymerase chain reaction on boiled clinical samples for rapid viral diagnosis. | an assessment of optimal conditions for rapid simultaneous amplification of multiple human papillomavirus (hpv) sequences has been made using thermus aquaticus dna polymerase. all variables of practical value were studied by amplifying known target-sequences from ten-fold dilutions of well characterized cell lines. in our hands, fast multiplex pcr (fm-pcr), the technique of running multiple pcr reactions simultaneously with minimum incubation time at each temperature, was highly sensitive (ampli ... | 1990 | 2175751 |
| transforming growth factors beta 1 and 2 transcriptionally regulate human papillomavirus (hpv) type 16 early gene expression in hpv-immortalized human genital epithelial cells. | human papillomavirus type 16 (hpv16) early proteins e6 and e7 have been implicated in maintenance of the malignant phenotype in cervical cancer. transforming growth factors beta one and two (tgf betas 1 and 2), polypeptides that regulate cellular growth and differentiation, reversibly inhibited expression of the hpv16 e6 and e7 genes in several immortal genital epithelial cell lines. loss of e6 and e7 protein expression followed a dramatic time- and dose-dependent decrease in e6 and e7 rna level ... | 1990 | 2168964 |
| a member of the activator protein 1 family found in keratinocytes but not in fibroblasts required for transcription from a human papillomavirus type 18 promoter. | papillomaviruses are tissue specific and replicate in differentiating keratinocytes. we are interested in the question of tissue specificity at the level of transcription. we used extracts from human keratinocytes and human fibroblasts at low passage number and from hela cells to look for factors binding to the e6 promoter of human papillomavirus type 18 (hpv-18) dna by footprint and gel mobility shift experiments. we found a factor present in hela and keratinocyte extracts but not in fibroblast ... | 1990 | 2168967 |
| human papillomavirus transmission and carcinogenesis. | 1990 | 2169235 | |
| oral focal epithelial hyperplasia removed with co2 laser. | a case of oral focal epithelial hyperplasia (feh) treated with co2 laser surgery is presented. histological diagnosis is discussed. the association of human papillomavirus (hpv) type 32 with the lesions is demonstrated with dna in situ hybridization technique. laser surgery is suggested as a treatment of choice. | 1990 | 2170540 |
| [study on the localization of human papillomavirus dna in the vulvar disease by means of in situ hybridization]. | we investigated human papillomavirus (hpv) dna of vulvar diseases obtained from 18 cases of condyloma acuminatum, 3 cases of hyperplastic dystrophy without atypia, 4 cases of lichen sclerosus, 4 cases of bowenoid papulosis, one case of bowen disease and 8 cases of squamous cell cancer by in situ hybridization with biotinylated hpv 6, 11, 16 and 18 dna probes. the results of in situ hybridization analysis showed that hpv 6/11 was positive in 94.4% (17/18) of condyloma acuminatum. in 17 cases of h ... | 1990 | 2170544 |
| [the role of human papillomaviruses among the risk factors for cervical carcinoma]. | in a prospective study, risk factors of the development of cervical carcinoma in 210 diseased and 100 healthy subjects were investigated. special attention was paid to metaplastic changes in the portio vaginalis cervics and to human papilloma virus which are considered responsible for the development of these changes. it has been found that metaplasias are favoured by adolescence, chronic irritative processes in the cervix (pathologic discharge recorded in 51.9% of the diseased and 38% of the he ... | 1990 | 2170775 |
| the e7 protein of human papillomavirus 8 is a nonphosphorylated protein of 17 kda and can be generated by two different mechanisms. | the e7 protein of human papillomavirus (hpv) 8 shows no in vitro transforming activity, in contrast to e7 of the genital hpvs, but seems to be involved in the control of viral dna replication. to determine whether functional differences between the e7 proteins of hpv16 and hpv8 were reflected in differences in their biochemical properties, we characterized the e7 protein of hpv8 expressed from the late simian virus 40 promoter in cos 7 cells. an e7-specific antiserum was obtained by immunizing r ... | 1990 | 2171214 |
| human cervical cells immortalized in vitro with oncogenic human papillomavirus dna differentiate dysplastically in vivo. | human papillomavirus (hpv) types 16 and 18 are associated with cervical dysplasia and carcinoma. in vitro integration of hpv-16 or hpv-18 dna into cultured human cervical cells results in their immortalization. in this study, in vivo differentiation of human cervical cells immortalized with recombinant hpv-16 or hpv-18 dna was studied by implanting confluent monolayers of hpv-16 and hpv-18 dna immortalized cell lines under dorsal skin flaps of immunodeficient mice. grafts of a human cervical can ... | 1990 | 2172120 |
| sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation. | the sensitivity of digoxigenin and biotin labelled dna probes for the detection of human papillomavirus (hpv) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled with biotin by nick translation and detected with streptavidin, both methods having a common final visualisation procedure using alkaline p ... | 1990 | 2172319 |
| [studies on pathogenesis of cervical carcinoma based on the analysis of growth and differentiation mechanism of cervical epithelium]. | recently cervical cancer is defined as a sexually-transmitted disease, and human papillomavirus (hpv) has been focused as one of its etiologic agents. it is known that cervical cancer is extraordinarily rare in non-human mammals that have the estrous cycle. in contrast, cervical cancer is frequent in human beings which have lost the estrous cycle, and subsequently evolved a sexual behavior irrespective of the menstrual phase. therefore, upon the hypothesis that the estrous cycle is a period prot ... | 1990 | 2172418 |
| [molecular biologic study on the carcinogenesis of hpv in uterine cervical cancer and related lesions--analysis of hpv types 16, 18 e6/e7 gene mrna]. | recent molecular biologic studies have shown that some type of human papillomavirus (hpv) is closely associated with uterine cervical cancer. in order to investigate the relationship between hpv dna and cervical cancer, we studied the carcinogenesis of hpv in cervical cancer and related lesions by dot blot hybridization (d.b.h.), southern blot hybridization (s.b.h.), in situ hybridization (i.s.h.) and polymerase chain reaction (p.c.r.). we also followed some hpv dna, mrna positive or negative ca ... | 1990 | 2172419 |
| human papillomavirus type 8 contains cis-active positive and negative transcriptional control sequences. | human papillomavirus type 8 (hpv-8) is one aetiological agent of macular and flat wart-like lesions in patients with epidermodysplasia verruciformis and appears to be closely linked to skin carcinogenesis. a 1.2 kb region of the genome, which was previously shown to contain a viral e2-dependent enhancer, was progressively shortened from both ends with bal 31. the resulting fragments were tested for their ability to stimulate chloramphenicol acetyltransferase (cat) expression from the simian viru ... | 1990 | 2172459 |
| effect of perilesional injection of alpha-interferon on cervical intraepithelial neoplasia and associated human papillomavirus infection. | in this controlled prospective study, 14 patients with cervical intraepithelial neoplasia (cin) were observed for one month to exclude spontaneous regression, and then seven patients were treated twice-weekly with perilesional injections of alpha-interferon. both groups were monitored colposcopically, and advised to use the contraceptive sheath. cervical smears and biopsies for histology and viral studies were taken before and after the study. there was an improvement of six out of seven of the ... | 1990 | 2172535 |
| comparison of southern transfer hybridization and dot filter hybridization for detection of cervical human papillomavirus infection with types 6, 11, 16, 18, 31, 33, and 35. | a commercial dot filter hybridization kit (virapap kit) was compared with southern transfer hybridization for the detection of seven types of human papillomavirus (hpv) in cervical specimens from 450 consecutive females attending a sexually transmitted diseases clinic. in comparison with southern transfer hybridization, performed with the same probes used in the dot filter kit, the sensitivity, specificity, and positive and negative predictive values of dot filter hybridization were 90%, 94%, 74 ... | 1990 | 2173397 |
| survey of histologic specimens of human cancer for human papillomavirus types 6/11/16/18 by filter in situ hybridization. | histologic specimens (317) of genital and nongenital cancers and normal tissue were analyzed for the presence of the dna of human papillomavirus (hpv) 6, 11, 16, and 18 by filter in situ hybridization performed on paraffin-embedded, formalin-fixed tissue (histofish). hpv dna was found in 73 of 172 (42%) anogenital lesions and 17 of 116 (15%) nonanogenital carcinomas. no hpv dna was found in normal mouse skin (five samples), human autopsy liver (two samples), or kidney (eight samples), or in carc ... | 1990 | 2173398 |
| time trends in the prevalence of human papillomavirus infections in archival papanicolaou smears: analysis by cytology, dna hybridization, and polymerase chain reaction. | a retrospective study was undertaken to determine the prevalence of human papillomavirus (hpv) infections in routine papanicolaou (pap) smears collected by general practitioners from western australian women in each of the years 1972, 1982, and 1987. hpv infection was detected by cytology, dot-blot hybridization, or polymerase chain reaction (pcr). it was found that the prevalence of hpv infection remained unchanged over the 15 year study period, was independent of age, and was associated with n ... | 1990 | 2173733 |
| cytologic correlates of cervical papillomavirus infection. | a fundamental question in papanicolaou smear screening is the specificity of cytologic criteria for the recognition of genital human papillomavirus (hpv) infection. to address this problem, we conducted a two-phase study of routinely screened women to determine the efficiency with which cytologic findings identified the presence of hpv dna, focusing on the criteria for identifying smears as "atypical." in phase 1, 25 of 290 (8.6%) smears were designated atypical, but only 3 (12%) of the samples ... | 1990 | 2174025 |
| genetic strategies of tumor suppression. | the evaluation of the cancer cell is a complex multigene process. tumor suppressor genes that are lost or inactivated, as well as genes that are overexpressed, play key roles in tumor progression. the identification of overexpressed genes has been expedited by the presence of transforming genes in some animal retroviruses. however, tumor suppressor genes have been difficult to identify and isolate because of their loss or inactivation during tumorigenesis. by a variety of methods, summarized in ... | 1990 | 2174661 |
| synthetic peptides derived from e7 region of human papillomavirus type 16 used as antigens in elisa. | nine overlapping peptides (20 amino acid) covering the entire sequence of early antigen e7 of human papillomavirus type 16 (hpv-16) were synthesized and tested as antigens with human sera in elisa. five of these peptides (no. 1 to 5 counting from the n terminus of the e7 protein) reacted with a pool of sera from hpv-16-infected individuals (as determined by molecular hybridization with their biopsy specimens); one (no. 5) was also reactive with pools of hpv-18- and hpv-6- or 11-infected individu ... | 1990 | 2174961 |
| human papillomavirus infection in sexually active adolescent females: prevalence and risk factors. | we examined the prevalence of human papillomavirus (hpv) infection, and associated risk factors for infection with hpv types 6, 11, 16, 18, 31, 33, and 35, in 661 sexually active adolescent females attending family planning clinics. fifteen percent were positive for hpv dna by rna-dna dot-blot hybridization. more than 60% of the hpv-positive subjects harbored at least one of the following cancer-related hpv types: 16, 18, 31, 33, or 35. those with hpv had a mean range of four to 10 lifetime sexu ... | 1990 | 2175024 |
| uterine cervical punch biopsy specimens can be analyzed by 1h mrs. | biopsy specimens of the uterine cervix, including colposcopically directed punch biopsy specimens of females with atypical papanicolaou smear tests, are suitable for analysis by magnetic resonance (mr) spectroscopy. a narrow lined lipid mr spectrum, characteristic of malignant tissue, is obtained from a 6-mm3 biopsy specimen of histologically confirmed squamous carcinoma of the cervix. in contrast, specimens containing inflammatory cells generate a broad component only centered at 1.3 ppm with a ... | 1990 | 2314221 |
| vestibular papillae of the vulva. lack of evidence for human papillomavirus etiology. | vestibular papillae of the vulva are usually considered as anatomical variants of the vestibular mucosa. clinically, however, they are quite often interpreted as condylomata acuminata and recent studies have suggested that they could be related to human papillomavirus infection. this prompted us to search for human papillomavirus dna using the southern blot hybridization technique, by analyzing biopsy specimens taken from 29 patients who presented with papillae of the vestibular mucosa. human pa ... | 1990 | 2175164 |
| human papillomavirus associated with keratoacanthomas in australian patients. | 1990 | 2175166 | |
| [detection of human papillomavirus dna in the normal genitalia of men]. | the study of human papillomavirus (hpv) in the genitalia of all normal males is difficult, therefore cases of chronic prostatitis, a common disease treated in urological clinics, were chosen to identify hpv dna by using vira type (life technologies inc.). smears of glans and sulcus coronalius of 177 subjects, showed a hpv positive rate of 3.4%, while 86 cases of those cases were negative for hpv in urethral smears. the lack of clinical findings suggests that hpv is an asymptomatic infection. in ... | 1990 | 2175330 |
| cervical intraepithelial neoplasia iii in an adolescent with bowenoid papulosis. | bowenoid papulosis (bp) is a cutaneous condition of the external genitalia seen primarily in young adults. evidence supports an etiologic role of human papillomavirus (hpv), particularly type 16. hpv-16 is also associated with an increased risk for cervical intraepithelial neoplasia (cin) and invasive carcinoma. a 17-year-old female, referred to the adolescent dysplasia clinic with a diagnosis of condyloma acuminatum, was presented. she had multiple smooth, shiny, brown 3- to 4-mm papules on the ... | 1990 | 2175743 |
| human papillomavirus in oesophageal squamous cell carcinoma. | thirty seven cases of oesophageal squamous cell carcinoma were studied by applying dna slot blot analysis and in situ hybridisation using type specific probes for hpv 6, 11, 16 and 18. cases of condyloma accuminata, cervical carcinoma, and laryngeal papilloma were used as controls. blocks including areas of invasive carcinoma, intraepithelial neoplasia, and normal epithelium were studied in each case. no hpv genome was detectable in any of the oesophageal cases. it is concluded that these types ... | 1990 | 2175754 |
| cell differentiation-related gene expression of human papillomavirus 33. | the gene expression of human papillomavirus (hpv) 33, which can be detected both in benign and malignant genital tumors, was analyzed in a cervical condyloma acuminatum by in situ hybridization using open reading frame-specific rna probes. viral mrna concentrations increased with the degree of differentiation of the keratinocytes. the probes for reading frames e4 and e5 generated the most intense signals. the patterns of the specific viral mrnas were very similar to those in condylomas induced b ... | 1990 | 2175837 |
| outcome in mild and moderate cervical dysplasias related to the presence of specific human papillomavirus types. | in situ hybridization with biotinylated dna viral probes (ish-b) for human papillomavirus (hpv) types 6/11, 16, 18, 31, and 33 was used to study the outcome in 32 cases of mild and 21 cases of moderate cervical dysplasia with koilocytotic change that were followed for an average of 27 mo. the rates of regression, persistence, and progression for cervical intraepithelial neoplasia (cin) i and cin ii were 50%, 41%, and 9%, and 43%, 48%, and 9%, respectively. while progression of hpv 16 cin i and i ... | 1990 | 2175897 |
| [detection of human papillomavirus (hvp) 16/18 infections of the vaginal uterine cervix in females before and following conisation]. | 280 women have been tested by fish to detect an hpv 16/18-infection of the portio uteri. in a group of 133 women treated by conisation (all with a history of abnormal smear and colposcopy) 67 (50%) were positive for hpv 16/18 dna before the operation. follow up examinations of 20 cases with positive hpv 16/18 test before conisation, showed 6 and 12 months after operation only in 3 cases a persistence of hpv 16/18 infection. only 5 (6.4%) of 78 women with a history of conisation and diagnosis cin ... | 1990 | 2176023 |
| antibody-mediated neutralization in vivo of infectious papillomaviruses. | specific antibody-mediated neutralization of infectious human papillomavirus type 11 (hpv-11) was achieved in the athymic mouse xenograft system, in which hpv-11 induced morphological transformation of human foreskin. virus-specific neutralization was demonstrated by the ability of an hpv-11-specific polyclonal antiserum to neutralize hpv-11 infectivity and not bovine papillomavirus type 1 (bpv-1) or cottontail rabbit papillomavirus (crpv) infectivity. in all three virus infectivity systems, neu ... | 1990 | 1693698 |
| humoral assays of human sera to disrupted and nondisrupted epitopes of human papillomavirus type 1. | the use of different assay systems and the disparity in results obtained has meant that we have little understanding about the role played by the humoral response during human papillomavirus (hpv) infection. human antibody responses have so far appeared to be largely directed against the major capsid protein, l1. this protein possesses both type-specific and type-common antigenic determinants but it is not known which of these is important in vivo during the natural course of infection. in this ... | 1990 | 1689524 |
| in vitro differentiation of epithelial cells from cervical neoplasias resembles in vivo lesions. | cell lines derived from cervical neoplasias, as well as cells from normal cervix and neonatal foreskin were examined in an in vitro culture system (raft system) that allows for stratification and differentiation of epithelial cells at an air-liquid interface. epithelial cells from human foreskin and ectocervix were observed to differentiate in a manner histologically similar to normal epithelium in vivo as indicated by a single layer of basal cells with a defined mid and upper zone. in contrast, ... | 1990 | 1691480 |
| growth-regulating functions of human papillomavirus early gene products in cervical cancer cells acting dominant over enhanced epidermal growth factor receptor expression. | squamous cell carcinomas of the human anogenital tract are usually associated with infection of specific types of human papillomaviruses (hpv 16, 18, 31, 33, 35). the intracellular concentration of human papillomavirus early gene products e6 and e7 has been directly linked to the proliferative capacity of cervical cancer cells. since the expression rate of epidermal growth factor receptor correlates to growth properties in squamous carcinoma cell lines, it has been presumed that human papillomav ... | 1990 | 1692767 |
| expression of cytokeratin no. 19 polypeptide in genital papillomavirus lesions. | a series of 23 punch biopsies proved to contain human papillomavirus (hpv) type 16 and with established clinical course (including hpv-ncin, hpv-cin i, and hpv-cin ii lesion), and 18 additional biopsies of hpv 6-, 11-, 16- or 18-induced genital lesions were analyzed immunohistochemically for expression of cytokeratin no. 19 polypeptide. an immunoperoxidase-abc technique was used with a polyclonal antibody raised against a synthetic nonapeptide corresponding to the residues 2-10 of the nh2-end, n ... | 1990 | 1693587 |
| identification of b epitopes in human papillomavirus type 16 e7 open reading frame protein. | human papillomavirus (hpv) type 16 is implicated in the aetiology of anogenital dysplasia which may progress to malignancy. hpv-16 dna is actively transcribed in cervical carcinomas, the most abundant transcripts being from the e6 and e7 early open reading frames. the e7 protein has been shown to have transforming activity in vitro. in this report we define four immunodominant b epitopes within the protein corresponding to the e7 gene, using a panel of murine monoclonal antibodies. three epitope ... | 1990 | 1693666 |
| immortalization of primary rat embryo cells by human papillomavirus type 11 dna is enhanced upon cotransfer of ras. | transfer of human papillomavirus type 11 (hpv11) dna and a neo(r) marker into primary rat embryo cells (rec) led to colony formation in g418-selective medium. about 20% of hpv11 clones were eventually established in culture but displayed low growth rates. cotransfection of hpv11 dna and an activated ras oncogene led to formation of both drug-resistant flat colonies and phenotypically transformed clones which grew efficiently when expanded in culture. a number of transformants reverted to a flat, ... | 1990 | 1695409 |
| selective suppression of human papilloma virus type 18 mrna level in hela cells by interferon. | we have studied the effect of ifn on the transcription of integrated hpv 18 dna in hela cells. we found that the amount of cytoplasmic hpv 18 mrna was markedly reduced in hela cells treated with 10(3) iu/ml of ifn alpha or gamma, and that the inhibitory effect was dose dependent and was detectable by 12h after the addition of ifn. furthermore, northern blot analysis indicated that ifn remarkably reduced 3.4 kb mrna species of hpv 18 transcripts in hela cells. in contrast, the level of beta-actin ... | 1990 | 1696477 |
| a method for detection of human papillomavirus dna in stored slides of stained cervical smears. | a simple method was developed for extraction of dna from stored slides of papanicolaou stained cervical smears. hpv type 16 dna was detected by dna hybridization in 7/10 samples from known hpv-16 positive patients, whereas the 10 samples from negative patients were also negative with our assay. using our method for obtaining data from stained cervical smears, it will be possible to design and perform historical cohort studies on stored material. | 1990 | 1705024 |
| hybricomb. a novel diagnostic tool for dna probing. | a technique is described in which unlabeled dna probes are immobilized on the plastic surface of a 12-tooth comb and used to capture homologs by a hybridization procedure. in the examples described, cellular dna from cervical biopsies is chemically labeled using the chemi-probe system. the sample containing labeled dna is then hybridized with the immobilized unlabeled probe (reverse hybridization). by means of this technique, human papillomavirus sequences can be detected with a sensitivity comp ... | 1990 | 1963055 |
| transcriptional activation of the human papillomavirus-16 p97 promoter by an 88-nucleotide enhancer containing distinct cell-dependent and ap-1-responsive modules. | the p97 promoter upstream of the oncogenic early genes of human papillomavirus (hpv)-16 is active in keratinocytes and in cervical carcinoma cells due to a 5' keratinocyte-dependent cis enhancer. in this study, we have mapped the main enhancer activity to an 88-nucleotide (nt) fragment composed of multiple cis elements. a 63-nt promoter-proximal enhancer core was sufficient for p97 activation in a human keratinocytic cell line, hacat, and in cervical carcinoma cells. although the enhancer functi ... | 1990 | 1963084 |
| [studies of human papillomavirus (hpv) in urological tumors]. | urological tumors were examined for the presence of human papillomavirus (hpv) dna by using southern blot hybridization. in 20 male patients with condyloma acuminatum, hpv type 6 was found at 85% (17/20), hpv type 11 at 95% (19/20), hpv type 16 at 5% (1/20) and hpv type 18 at 0% (0/20). in 2 female patients with condyloma acuminatum, hpv types 6, 11, 16 and 18 were found at 100% (2/2), 100% (2/2), 50% (1/2) and 0% (0/2), respectively. all 6 of the patients who were positive for hpv type 6, were ... | 1990 | 1962828 |
| [in situ hybridization study of human papillomavirus from the penile cancer]. | the histochemical detection of human papillomavirus (hpv) was performed using the technique of in situ hybridization from the formalin fixed paraffin embedded specimens of penile carcinoma and condylomata+ acuminata . one of 19 penile cancer cases (5.3%) revealed the positive staining on the nuclei of the tumors for hpv type 16/18. however, the positive nuclei were scattered and localized in small part of the tumor which showed no koilocytosis. on the contrary, 11 of 12 condyloma acuminata cases ... | 1990 | 1962829 |
| herpes simplex and human papillomavirus genital infections: controversy over obstetric management. | plasma inhibitory factors, high levels of sex hormones, and depression of cell-mediated immunity may interfere with the natural host resistance to viral infections during pregnancy. it is apparent that hormonal, immunologic, and vascular changes in pregnancy may account for increased replication of herpes and for enhanced growth of condylomatous lesions. the challenge is to develop a rational plan of management for pregnant patients with herpes simplex or human papilloma virus infection. there h ... | 1990 | 1963124 |
| discrimination of closely homologous hpv types by nonisotopic in situ hybridization: definition and derivation of tissue melting temperatures. | it is generally assumed that nucleic acid association during in situ hybridization reactions is similar to that of nucleic acid association in solution. this assumption has been investigated by detecting closely homologous human papillomavirus types 6 and 11 by in situ hybridization as a model for the evaluation of stringency conditions in clinical biopsies. by examining matched and mismatched, labelled and target sequences under various stringency conditions, empirical dna-dna stability curves ... | 1990 | 1963174 |
| [relationship between human papillomavirus and oncogenes (c-myc, n-myc) amplification in human cervical cancers]. | the human papillomavirus detection and oncogenes amplifications were studied on dnas from fifteen cervical cancers. we detected hpv16 and hpv18 using southern blot hybridization and polymerase chain reaction (pcr) technique. the positive subjects of hpvs were eight cases (53%) observed by southern blot hybridization and fourteen cases (93%) by pcr technique. the gene amplifications of oncogenes (c-myc and n-myc) were analysed by slot-blot method and were observed in c-myc but not in n-myc. the " ... | 1990 | 1963632 |
| human papillomavirus dna from warts for typing by endonuclease restriction patterns: purification by alkaline plasmid methods. | the alkaline plasmid dna extraction method of birnboim and doly was applied for the isolation of human papillomavirus (hpv) from warts. tissue from common and plantar warts was digested with proteinase k, and the extrachromosomal circular covalently-closed form of hpv-dna was rapidly extracted by alkaline sodium dodecyl sulphate and phenol-chloroform treatment. recovery of hpv-dna from the tissue was sufficient for determination of endonuclease restriction patterns by agarose gel electrophoresis ... | 1990 | 1964033 |
| precursor lesions of the cervix. | screening has played a major role in decreasing the morbidity and mortality associated with carcinoma of the cervix. aggressive management of lesions recognized as precursor to invasive squamous carcinoma, (cervical intraepithelial neoplasia), has markedly reduced the incidence of invasive cancer. squamous carcinomas constitute most of the cervical cancers. the relationship between human papilloma virus and cervical abnormalities is reviewed. nursing diagnoses and care planning ideas are discuss ... | 1990 | 1964388 |
| a comparative sequence analysis of two human papillomavirus (hpv) types 2a and 57. | hpv 2a is commonly associated with verrucae vulgares, whereas hpv 57 was detected in mucosal lesions of the maxillary sinus and the genital tract, as well as in cutaneous lesions. the complete dna sequences of hpv 2a and hpv 57 were determined. the hpv 2a genome consists of 7860 base pairs and the hpv 57 genome contains 7861 base pairs. on the nucleotide level an 83% homology between the two sequences could be ascertained. compared to other hpvs they have a high g/c-content (hpv 2a: 48.8%, hpv 5 ... | 1990 | 1964523 |