Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| increased expression of vascular endothelial growth factor (vegf) in castleman's disease: proposed pathomechanism of vascular proliferation in the affected lymph node. | castleman's disease is a lymphoproliferative disorder of unknown etiology characterized by enlarged hyperplastic lymph nodes with marked vascular proliferation. to evaluate the possible involvement of vascular endothelial growth factor (vegf) in the pathogenesis of castleman's disease, we studied vegf expression in sera and lymph nodes from four patients with either the plasma-cell type or mixed type of castleman's disease. clinically, one patient had the multicentric type and the others the loc ... | 2000 | 10830746 |
| comparison of serologic assays and pcr for diagnosis of human herpesvirus 8 infection. | a variety of assays for the diagnosis human herpesvirus 8 (hhv-8) infection have been reported. we compared several such assays with a panel of 88 specimens from human immunodeficiency virus (hiv)-infected patients with kaposi's sarcoma (ks) (current-ks patients; n = 30), hiv-infected patients who later developed ks (later-ks patients; n = 13), hiv-infected patients without ks (no-ks patients; n = 25), and healthy blood donors (n = 20). pcr assays were also performed with purified peripheral blo ... | 2000 | 10834972 |
| interaction of human immunodeficiency virus type 1 and human herpesvirus type 8 infections on the incidence of kaposi's sarcoma. | to determine kaposi's sarcoma (ks) risk related to timing of human immunodeficiency virus type 1 (hiv-1) and human herpesvirus type 8 (hhv-8) infections, stored longitudinal sera from 400 homosexual men with known dates of hiv-1 seroconversion (+/-4.5 months) were tested for hhv-8 antibody. times from hhv-8 seroconversion to ks were compared for the 69 men who became infected with hhv-8 after acquiring hiv-1 to the 182 men who were hhv-8 seropositive before their hiv-1 infection. none developed ... | 2000 | 10837173 |
| high prevalence of epstein-barr virus type 2 among homosexual men is caused by sexual transmission. | to investigate whether epstein-barr virus (ebv) type 2 infection is highly prevalent among homosexual men, the prevalence of ebv type 2 was studied among homosexual and heterosexual white men who were at high and low risk for sexually transmitted diseases; these data were correlated with sexual behavior. the prevalence of ebv type 2 among homosexual men was significantly higher than it was among heterosexual men (39% vs. 6%). among high-risk heterosexual men, prevalence was significantly higher ... | 2000 | 10837190 |
| selective elimination of high constitutive activity or chemokine binding in the human herpesvirus 8 encoded seven transmembrane oncogene orf74. | open reading frame 74 (orf74) encoded by human herpesvirus 8 is a highly constitutively active seven transmembrane (7tm) receptor stimulated by angiogenic chemokines, e.g. growth-related oncogene-alpha, and inhibited by angiostatic chemokines e.g. interferon-gamma-inducible protein. transgenic mice expressing orf74 under control of the cd2 promoter develop highly vascularized kaposi's sarcoma-like tumors. through targeted mutagenesis we here create three distinct phenotypes of orf74: a receptor ... | 2000 | 10842179 |
| kaposi's sarcoma-associated herpesvirus gene sequences are detectable at low copy number in primary amyloidosis. | primary amyloidosis (al), like multiple myeloma (mm), results from a clonal proliferation of plasma cells. recent detection of kaposi's sarcoma-associated herpesvirus (kshv) gene sequences in mm patients, although controversial, suggested that kshv may also be present in al. in the present study, we assayed for kshv gene sequences in patients with primary al independently in 2 laboratories. nested polymerase chain reaction (pcr) was performed on dna isolated from 21 bone marrow (bm) core biopsy ... | 2000 | 10842716 |
| absence of herpesvirus dna sequences in the 5t murine model of human multiple myeloma. | kaposi's sarcoma-associated herpesvirus (kshv, also known as hhv-8) has been found in patients with multiple myeloma (mm) and postulated to be aetiologically associated with the development of this common plasma cell malignancy. a murine model of mm was previously established in which intravenous transfer of 5t myeloma cells into c57bl/kalwrij mice resulted in characteristic features of human mm. in the present study, we sought to identify herpesvirus dna sequences in this murine model of mm thr ... | 2000 | 10848833 |
| human herpes virus 8 interleukin-6 homologue triggers gp130 on neuronal and hematopoietic cells. | human herpes virus-8 (hhv8) encodes a cytokine named viral interleukin-6 (vil-6) that shares 25% amino-acid identity with its human homologue. human il-6 is known to be a growth and differentiation factor of lymphatic cells and plays a potential role in the pathophysiology of various lymphoproliferative diseases. vil-6 is expressed in hhv8-associated-diseases including kaposi's sarcoma, body-cavity-based-lymphoma and castleman's disease, suggesting a pathogenetic involvement in the malignant gro ... | 2000 | 10848977 |
| spontaneously regressed kaposi's sarcoma and human herpesvirus 8 infection in a human immunodeficiency virus-negative patient. | kaposi's sarcoma occurring in a 78-year-old woman, with the absence of the human immunodeficiency virus infection, was correctly diagnosed by immunohistochemistry using anti-human herpesvirus 8 (hhv8) antibody (pa1-73n) for the first time. the patient suffered from chronic respiratory failure and was treated with a low dose of steroids for 2.5 years. after her medication dosage was increased for the exacerbation of the respiratory failure, multiple skin tumors in her feet and legs suddenly devel ... | 2000 | 10849322 |
| human herpesvirus 8 infection of the genital tract of hiv-seropositive and hiv-seronegative women at risk of sexually transmitted diseases. canadian women's hiv study group. | 2000 | 10853998 | |
| human herpesvirus-8-associated disseminated angiosarcoma in an hiv-seronegative woman: report of a case and limited case-control virologic study in vascular tumors. | 2000 | 10856415 | |
| detection of lymphotropic herpesvirus dna by polymerase chain reaction in cerebrospinal fluid of aids patients with neurological disease. | cerebrospinal fluid (csf) samples from 49 acquired immunodefficiency disease syndrome (aids) patients with a central nervous system (cns) disease were examined by polymerase chain reaction (pcr) to evaluate the association between the positivity for cytomegalovirus (cmv) and epstein-barr virus (ebv), and clinical diagnosis of a cns disease. frequency and clinical relevance of detection of dna of human herpesviruses 6 (hhv-6), 7 (hhv-7) and 8 (hhv-8) were also determined. dna of one or more of th ... | 2000 | 11155355 |
| human herpesviruses-6, -7 and -8 in organ transplant recipients. | the newer herpesviruses are being increasingly recognized as significant opportunistic pathogens in organ transplant recipients. published data support the role of human herpesvirus-6 as a potential cause of encephalitis and bone marrow suppression in transplant setting. an association of human herpesvirus-6 with fungal infections and cytomegalovirus infection has also been documented. human herpesvirus-7 also appears to be an immunomodulatory agent and may facilitate the pathogenicity of cytome ... | 2000 | 11168178 |
| [primary effusion lymphoma and other lymphoproliferative diseases associated with hhv-8]. | 2000 | 11198465 | |
| a review of topical and intralesional cidofovir. | cidofovir is a potent nucleoside analog antiviral drug approved for the treatment of cytomegalovirus (cmv) retinitis in patients with the acquired immune deficiency syndrome (aids). it is currently available only for intravenous infusion. several small studies and case reports describe the successful use of cidofovir applied either topically or by intralesional injection in several virally induced cutaneous diseases. available information demonstrates that cidofovir is a potent antiviral agent w ... | 2000 | 11328613 |
| exogenous fibroblast growth factor-2 induces a transformed phenotype in vascular kaposi's sarcoma-like cells. | vascular ttb cells derive from murine kaposi's sarcoma-like dermal lesions and share several phenotypic features with aids-associated ks spindle cells. we have recently reported that fibroblast growth factor-2 (fgf-2) promotes dramatic cytoskeletal and morphological alterations in ttb cells, concomitant with the induction of an autocrine loop for hepatocyte growth factor and a relocalization of the urokinase receptor. since all these alterations are hallmarks of cell transformation. we attempted ... | 2000 | 11409912 |
| is volcanic soil a cofactor for classic kaposi's sarcoma? | 2000 | 11484811 | |
| inflammatory cytokines and the reactivation of kaposi's sarcoma-associated herpesvirus lytic replication. | kaposi's sarcoma (ks) is a complex proliferative lesion long suspected of being dependent on exogenous paracrine signaling molecules to stimulate its proliferative, angiogenic, and inflammatory components. in particular, both clinical and experimental observations have pointed to a potential role for inflammatory cytokines as permissive factors for ks development, but ks pathogenesis is also critically dependent on infection by an exogenous herpesvirus, the ks-associated herpesvirus (kshv). to e ... | 2000 | 10612656 |
| kaposi sarcoma of major salivary gland origin: a clinicopathologic series of six cases. | kaposi sarcoma (ks), one of the defining tumors of acquired immune deficiency syndrome (aids), is rarely identified in the major salivary glands. to the authors' knowledge, no previous published series has evaluated the clinicopathologic aspects of this tumor in major salivary glands. | 2000 | 10618601 |
| human immunodeficiency virus-seropositive individual with persistent human herpesvirus 8 infection for >11 years without development of kaposi's sarcoma. | 2000 | 10619767 | |
| immunomodulatory therapy in the management of viral infections in patients with hiv infection. | human immunodeficiency virus (hiv) causes disease by infecting lymphocytes and progressively destroying critical regulatory and effector cells of the immune system, leaving patients vulnerable to a number of bacterial, fungal, and viral infections. facial herpes (herpes simplex virus-1 [hsv-1]), genital herpes (hsv-2), herpes zoster (varicella zoster virus), oral hairy leukoplakia (epstein-barr virus), kaposi's sarcoma (hhv-8), molluscum contagiosum, condyloma acuminata (human papillomavirus [hp ... | 2000 | 10861104 |
| human herpesvirus 8-encoded interleukin-6 homologue (viral il-6) induces endogenous human il-6 secretion. | we found that human herpesvirus 8-encoded il-6 (vil-6) induced endogenous human il-6 (huil-6) secretion from various cell lines (mt-4, thp-1, u937, raji, and cess) including patients with multicentric castleman's disease. especially, in mt-4 cells, huil-6 was enhanced with vil-6 by 30-fold compared with that of control. in addition, reverse transcriptase-polymerase chain reaction confirmed that vil-6 induced huil-6 expression in mt-4 cells. our novel finding of the huil-6 induction by vil-6 indi ... | 2000 | 10861641 |
| [hhv-8/kshv. i - epidemiological and molecular aspects]. | 2000 | 10863190 | |
| protein synthesis by solid-phase chemical ligation using a safety catch linker. | the native chemical ligation reaction has been used extensively for the synthesis of the large polypeptides that correspond to folded proteins and domains. the efficiency of the synthesis of the target protein is highly dependent on the number of peptide segments in the synthesis. assembly of proteins from multiple components requires repeated purification and lyophilization steps that give rise to considerable handling losses. in principle, performing the ligation reactions on a solid support w ... | 2000 | 10864771 |
| kaposi's sarcoma cells of different etiologic origins respond to hiv-tat through the flk-1/kdr (vegfr-2): relevance in aids-ks pathology. | kaposi's sarcoma (ks) is an hyperplastic lesion whose main histological features are typical spindle shaped cells with a mixed endothelial-mesenchymal-macrophage phenotype, an intense vascularization and an inflammatory infiltrate. the etiology of ks appears to be linked to activation of a latent hhv8 infection. sporadic and iatrogenic ks are slow progressing lesions that can undergo spontaneous regression. in contrast, ks, which is frequently associated with hiv infection, is found in a highly ... | 2000 | 10873597 |
| detection of the human herpesvirus 8-encoded cyclin protein in primary effusion lymphoma-derived cell lines. | the human herpesvirus 8 (hhv8/kshv), along with certain other herpesviruses, encodes a gene with cyclin homology. although the functional significance of the encoded cyclin is not clear at present, various lines of evidence propose a role for this cyclin in latently infected cells and possibly in the induction of tumors that arise in hhv8-infected individuals. we provide evidence here that the cyclin protein is expressed in hhv8 positive primary effusion lymphoma (pel)-derived cell lines and tha ... | 2000 | 10873769 |
| transfusion-associated infections with cytomegalovirus and other human herpesviruses. | of all human herpesviruses, human cytomegalovirus (hcmv) is the most significant cause of transfusion-associated (ta) morbidity and mortality. the problem of ta hcmv infection differs from that of other transfusion-transmitted infections in that only certain groups of patients require hcmv-free blood or blood components, i.e. seronegative pregnant women, premature infants of low birth weight who are born to seronegative mothers, seronegative recipients of allogeneic bone marrow transplants from ... | 2000 | 10878482 |
| inhibition of intracellular transport of b cell antigen receptor complexes by kaposi's sarcoma-associated herpesvirus k1. | the b cell antigen receptor (bcr) is a large complex that consists of a disulfide-linked tetramer of two transmembrane heavy (mu) chains and two light (lambda or kappa) chains in association with a heterodimer of igalpha and igbeta. kaposi's sarcoma-associated herpesvirus (kshv) encodes a transforming protein called k1, which has structural and functional similarity to igalpha and igbeta. we demonstrate that k1 downregulates the expression of bcr complexes on the surface. the nh(2)-terminal regi ... | 2000 | 10880522 |
| localisation of hhv-8 in aids related lymphadenopathy. | many lymph node abnormalities have been described in aids. these include opportunistic infections that sometimes result in spindle cell pseudotumours, kaposi's sarcoma (ks), malignant lymphoma (hodgkin's and non-hodgkin's), and florid reactive hyperplasia. among these, reactive hyperplasia is the most common manifestation of aids related lymphadenopathy. | 2000 | 10884921 |
| the restricted cellular host range of human herpesvirus 8. | a selection of primary and transformed cell types were evaluated for their susceptibility to infection with human herpesvirus 8 (hhv-8)/kaposi's sarcoma-associated herpesvirus. | 2000 | 10894276 |
| on the control of late gene expression in kaposi's sarcoma-associated herpesvirus (human herpesvirus-8). | herpesvirus late genes require viral dna replication for maximal expression. although late gene expression appears to require dna replication in cis in alphaherpesviruses, studies in epstein-barr virus (ebv) suggest that this cis-requirement might not pertain to the gammaherpesviruses. based on these findings, a system was created to investigate the elements required for the regulation of kaposi's sarcoma-associated herpesvirus (kshv; human herpesvirus-8) late gene expression. the transcript of ... | 2000 | 10900043 |
| human herpes virus-8 associated with two cases of primary-effusion lymphoma. | primary-effusion lymphoma (pel) is a rare form of non-hodgkin's lymphoma which predominantly occurs in patients with acquired immunodeficiency syndrome and is characterized by the presence of a malignant effusion in one or more of the body cavities, generally in the absence of a primary tumor mass. recently, we encountered two cases of pel presenting as cardiac tamponade. in both cases, a diagnosis of diffuse large b-cell lymphoma was made by examination of the pericardial fluid. because human h ... | 2000 | 10901615 |
| treatment of nonmelanotic hyperpigmentation with the q-switched ruby laser. | hyperpigmentation of the skin is often refractory to conventional therapies, but has significant cosmetic implications if located on visible areas. because laser systems are capable of removing pigment deposits caused by selective photothermolysis, we addressed the issue of whether the q-switched ruby laser could be a useful alternative in the treatment of nonmelanotic hyperpigmented skin lesions. we report the successful treatment of a patient with hyperpigmentation caused by iatrogenic human h ... | 2000 | 10906650 |
| characterization of a novel hhv-8-positive cell line reveals implications for the pathogenesis and cell cycle control of primary effusion lymphoma. | primary effusion lymphoma (pel) represents a peculiar type of b cell lymphoma which associates with hhv-8 infection and preferentially grows in liquid phase in the serous body cavities. in this report, we provide the detailed characterization of a newly established pel cell line, termed cro-ap/6. the cell line was obtained from the pleural effusion of a hiv-positive patient with pel. its derivation from the tumor clone was established by immunogenotypic analysis. detailed phenotypic investigatio ... | 2000 | 10914556 |
| cd19+ and cd20+ b cells from the peripheral blood of patients with multiple myeloma are not infected with human herpesvirus 8. | 2000 | 10914566 | |
| no evidence of active infection with human herpesvirus 6 (hhv-6) or hhv-8 in chronic fatigue syndrome. | 2000 | 10917774 | |
| decreased immunoreactivity for cell-cycle regulator p27(kip1) in kaposi's sarcoma correlates with higher stage and extracutaneous involvement. | a consistent relationship has been established between the development of kaposi's sarcoma (ks) and human herpes virus-8 (hhv8) infection. hhv8-encoded v-cyclin, through its complexing with cyclin-dependent kinase 6, contributes to the phosphorylation and proteasome-mediated degradation of p27(kip1). on the other hand, down-regulation of p27(kip1) expression seems to facilitate metastatic dissemination in a variety of human neoplasms. although the neoplastic nature of ks remains controversial, i ... | 2000 | 10918213 |
| a case of classic kaposi's sarcoma in a japanese man: detection of human herpes virus 8 (hhv-8) infection by means of polymerase chain reaction and immunofluorescence assay. | the recently discovered human herpes virus 8 (hhv-8) has been implicated in the pathogenesis of kaposi's sarcoma (ks). because classic ks in japan is rare and the detection of hhv-8 dna by polymerase chain reaction (pcr) has been successful only in limited cases, the frequency and role of hhv-8 infection in ks in japan remain unclear. herein we report a case of classic ks in a japanese man whose hhv-8 infection was confirmed by the detection of lesional viral dna and serum antibodies against lyt ... | 2000 | 10920585 |
| impaired ctl recognition of cells latently infected with kaposi's sarcoma-associated herpes virus. | kaposi's sarcoma-associated herpes virus (kshv) is a recently identified human gamma2-herpesvirus associated with kaposi's sarcoma, primary effusion lymphoma, and castleman's disease. we reasoned that ctl responses may provide host defense against this virus, and consequently, kshv may have evolved strategies to evade the ctl-mediated immune surveillance. in this study six b cell lines latently infected with kshv were found to express reduced levels of hla class i surface molecules compared with ... | 2000 | 10925292 |
| primary infection with human herpesvirus 8 in an hiv-1-infected patient. | 2000 | 10930174 | |
| does hhv-8 have a neuroprotective role on the development of hiv encephalopathy? | 2000 | 10932300 | |
| absence of immunohistochemical evidence for human herpesvirus 8 (hhv8) in oral cavity plasmablastic lymphoma in an hiv-positive man. | 2000 | 10942339 | |
| selenium and interleukins in persons infected with human immunodeficiency virus type 1. | an important role for selenium in human immunodeficiency virus (hiv) disease has been proposed. decreased selenium levels, as found in persons with hiv infection or aids, are sensitive markers of disease progression. selenium deficiency, an independent predictor of mortality in both hiv-1-infected adults and children, is an essential micronutrient that is associated with an improvement of t cell function and reduced apoptosis in animal models. in addition, adequate selenium may enhance resistanc ... | 2000 | 10944486 |
| the role of human immunodeficiency virus-i in the pathogenesis of acquired immunodeficiency syndrome-related kaposi's sarcoma: the importance of an inflammatory and angiogenic milieu. | people infected with human immunodeficiency virus type-i (hiv-i) have an increased incidence of kaposi's sarcoma (ks) and hiv-i infection alters the natural history of ks. the potent trans-activator hiv-i protein tat plays a major role in the pathogenesis of acquired immunodeficiency syndrome (aids)-related ks. among many of its ks-promoting activities, the tat protein augments the angiogenic activities of basic fibroblast growth factor (bfgf), interferon gamma, and vascular endothelial growth f ... | 2000 | 10950368 |
| new therapies for the treatment of aids-related kaposi sarcoma. | kaposi sarcoma (ks) is the most common tumor arising in hiv-infected patients and is an aids-defining illness by the centers for disease control guidelines. the clinical course of aids-related ks is highly variable, ranging from minimal stable disease to explosive growth. recent advances in the elucidation of the pathogenesis of ks are uncovering many potential targets for ks therapies. such targets include the processes of angiogenesis and cellular differentiation, sex hormones, and the ks herp ... | 2000 | 10975552 |
| kaposi's sarcoma. | 2000 | 10979790 | |
| [primary effusion lymphoma associated with type-8 human herpes virus infection]. | primary effusion lymphoma is an unusual entity and it has been described as a subset associated with human herpes virus 8 infection in homosexual males with aids. its inclusion as a new entity in the revised european-american lymphoma classification has been recommended. the case in which it is presented is a 47-year-old man, diagnosed with aids two years ago, who came with kaposi's sarcoma. nowadays, he has a right pleural effusion and a thoracentesis has been carried out. we obtain 10 ml of ha ... | 2000 | 10981334 |
| seroprevalence of human herpesvirus 8 and its association with kaposi sarcoma in brazil. | epidemiologic studies have indicated that human herpesvirus 8 is implicated in the development of kaposi sarcoma in different geographic areas worldwide. | 2000 | 10987454 |
| human herpesvirus 8 lana interacts with proteins of the msin3 corepressor complex and negatively regulates epstein-barr virus gene expression in dually infected pel cells. | the human herpesvirus 8 (hhv-8) latency-associated nuclear antigen (lana) is expressed in all latently hhv-8 infected cells and in hhv-8-associated tumors, including primary effusion lymphoma (pel). to better understand the contribution of lana to tumorigenesis and to the pel phenotype, we performed a yeast two-hybrid screen which identified the corepressor protein sap30 as a lana binding protein. sap30 is a constituent of a large multicomponent complex that brings histone deacetylases to the pr ... | 2000 | 11000236 |
| kshv-like herpesviruses in chimps and gorillas. | 2000 | 11001045 | |
| long latency of human herpesvirus type 8 infection and the appearance of classic kaposi's sarcoma. | 2000 | 11004646 | |
| herpesviruses 6, 7 and 8 in hiv- and non-hiv-associated periodontitis. | human herpesviruses, especially cytomegalovirus and epstein barr virus type-1, occur with higher frequency in subgingival specimens from periodontitis lesions than from healthy/gingivitis sites. little or no information is available on the relationship between herpesvirus 6 (hhv-6), herpesvirus 7 (hhv-7) and herpesvirus 8 (hhv-8) and periodontal disease. this study determined the periodontal occurrence of hhv-6, hhv-7 and hhv-8 in 21 hiv-seropositive and 14 hiv-negative adults affected by period ... | 2000 | 11005155 |
| kaposi's sarcoma-associated virus mounts novel attack on immune system. | 2000 | 11006519 | |
| human herpesvirus 8 in brazilian amerindians: does vertical transmission play a role? | 2000 | 11023487 | |
| inhibition of p300 histone acetyltransferase by viral interferon regulatory factor. | kaposi's sarcoma-associated herpesvirus (kshv) has been consistently identified in kaposi's sarcomas, body cavity-based lymphomas, and some forms of castleman's disease. the k9 open reading frame of kshv encodes a viral interferon regulatory factor (virf) which functions as a repressor for cellular interferon-mediated signal transduction and as an oncogene to induce cell growth transformation. we demonstrate that kshv virf directly interacts with cellular transcriptional coactivator p300 and dis ... | 2000 | 11027294 |
| human herpesvirus 8 is not associated with sarcoidosis in japanese patients. | the etiology of sarcoidosis remains unknown, but recently it was reported that human herpesvirus 8 (hhv-8) may be detected in sarcoid tissue in a high proportion of patients. this study was performed to determine whether hhv-8 is implicated in sarcoidosis in japan. | 2000 | 11035657 |
| detection of human herpesvirus 8 dna and antibodies to latent nuclear and lytic-phase antigens in serial samples from aids patients with kaposi's sarcoma. | human herpesvirus 8 (hhv-8) have recently implicated in the etiology of kaposi's sarcoma (ks), but the pathophysiologic and immunologic interactions between hhv-8 and the human host are incompletely understood. | 2000 | 10738143 |
| characterization of signaling cascades triggered by human interleukin-6 versus kaposi's sarcoma-associated herpes virus-encoded viral interleukin 6. | kaposi's sarcoma-associated herpes virus (kshv) is associated with kaposi's sarcoma, multicentric castleman's disease, and body cavity-based lymphomas, settings in which human interleukin-6 (hil-6) acts as a growth factor. the kshv open reading frame k2 encodes for viral il-6 (vil-6), a protein with 25% amino acid identity to hil-6, which can promote the growth of hil-6-dependent cell lines. in the present study, we characterized biological sequelae and signaling cascades triggered by hil-6 vers ... | 2000 | 10741750 |
| lack of detection of human herpesvirus (hhv)-8 dna in lesional skin of german pemphigus vulgaris and pemphigus foliaceus patients. | 2000 | 10745034 | |
| quantitative analysis of human herpesvirus 8 viral load using a real-time pcr assay. | we have developed a quantitative real-time pcr (taqman) assay aimed at measuring the cellular human herpesvirus 8 (hhv-8) dna load in various clinical samples. standard curves were obtained by serial dilutions of a control plasmid containing both hhv-8 (orf73 gene) and the cellular target (human albumin gene). the assay appeared to be very sensitive (100% detection rate for at least 10 copies per well) and specific and was easily reproducible (less than 3% intra-assay variability, 5% interassay ... | 2000 | 10747115 |
| two distinct gamma-2 herpesviruses in african green monkeys: a second gamma-2 herpesvirus lineage among old world primates? | primate gamma-2 herpesviruses (rhadinoviruses) have so far been found in humans (kaposi's sarcoma-associated herpesvirus [kshv], also called human herpesvirus 8), macaques (macaca spp.) (rhesus rhadinovirus [rrv] and retroperitoneal fibromatosis herpesvirus [rfhv]), squirrel monkeys (saimiri sciureus) (herpesvirus saimiri), and spider monkeys (ateles spp.) (herpesvirus ateles). using serological screening and degenerate consensus primer pcr for the viral dna polymerase gene, we have detected seq ... | 2000 | 10627572 |
| gammaherpesviruses and "hit-and-run" oncogenesis. | 2000 | 10623645 | |
| the human herpesvirus 8 homolog of epstein-barr virus sm protein (ks-sm) is a posttranscriptional activator of gene expression. | homologs of the epstein-barr virus (ebv) sm protein exist in several human and nonhuman herpesviruses. structure and function differ significantly among these proteins. we have cloned and characterized the human herpesvirus 8 (hhv8) gene, ks-sm, which is homologous to the ebv sm and herpes simplex virus icp27 genes, from an hhv8-infected primary effusion lymphoma. ks-sm is shown to be a posttranscriptional activator of gene expression in cotransfection studies. ks-sm activated gene expression in ... | 2000 | 10623771 |
| classic kaposi sarcoma: epidemiology and risk factors. | although kaposi sarcoma (ks) initially was described over a century ago, its biology remains enigmatic and conflicting. whereas the classic type occurs mainly in older men of mediterranean or eastern european backgrounds and is not linked to impairment of the host immune response, iatrogenic and human immunodeficiency virus (hiv)-associated ks are linked to such conditions. a recently discovered pathogen, ks-associated herpesvirus (kshv) (also known as human herpesvirus 8 [hhv8]), is found in ti ... | 2000 | 10649240 |
| danish patients with untreated multiple myeloma do not harbour human herpesvirus 8. | the role of human herpesvirus 8 (hhv-8) in multiple myeloma (mm) remains controversial. we examined 15 danish mm patients before cytoreductive therapy. mononuclear cells isolated from peripheral blood and bone marrow aspirates, as well as long-term cultured bone marrow stromal cells, were assayed for the presence of hhv-8 dna. all material was tested by three simple unnested polymerase chain reaction (pcr) assays (amplifying regions of orf26, orfk1 and orf75) and two nested pcr assays (amplifyin ... | 2000 | 10651731 |
| human herpesvirus 8-associated solid lymphomas that occur in aids patients take anaplastic large cell morphology. | human herpesvirus type 8 (hhv-8; kaposi's sarcoma-associated herpesvirus) is a recently isolated human herpesvirus frequently identified in kaposi's sarcoma, primary effusion lymphoma, and multicentric castleman's disease. here we report three cases of hhv-8-bearing solid lymphomas that occurred in aids patients (cases 1-3). all three patients were homosexual men presenting extranodal masses in the lungs (case 1) or skin (cases 2 and 3), together with the presence of kaposi's sarcoma (case 1), p ... | 2000 | 10658913 |
| differential expression of the hhv-8 vgcr cellular homolog gene in aids-associated and classic kaposi's sarcoma: potential role of hiv-1 tat. | human herpesvirus 8 (hhv-8) has been causally linked to kaposi's sarcoma (ks). there is significant homology between some hhv-8 genes and cellular genes including d-type cyclin (vcyc), g protein coupled receptor (vgcr), macrophage inflammatory proteins (vmip-i, vmip-ii), bcl-2 (vbcl2), interferon regulatory factor-1 (virf1), interleukin-6 (vil6), and complement-binding protein (vcbp). in this study, we analyzed expression of these viral homologs and hiv-1 tat by reverse-transcriptase polymerase ... | 2000 | 10662620 |
| risk factors for human herpesvirus 8 seropositivity and seroconversion in a cohort of homosexual men. | sexual and nonsexual modes of transmission of human herpesvirus 8 (hhv8) have been suggested, but specific routes remain unclear. therefore, the objective of this study was to assess risk factors for hhv8 seropositivity and determine specific sexual practices associated with hhv8 seroconversion. sera from 1,458 homosexual men (amsterdam cohort study, 1984-1996) were tested for antibodies to hhv8 with a modified version of an enzyme immunoassay, using recombinant hhv8 lytic phase capsid (orf65) a ... | 2000 | 10670545 |
| detection of antibodies to human herpesvirus 8 in italian children: evidence for horizontal transmission. | human herpesvirus 8 (hhv-8), also known as kaposi's sarcoma associated herpesvirus (kshv), has been shown to be the causative agent for kaposi's sarcoma (ks) and to be more prevalent in populations or risk groups at increased risk for ks. hhv-8 infection is rare in children from the us and the uk, but has been reported in african children. in this study we examine hhv-8 infection in children from italy, a country with an elevated prevalence of hhv-8 in adults and high socio-economic conditions. | 2000 | 10682685 |
| do intravenous immunoglobulin products manufactured from plasma collected in italy place immunocompromised patients at risk of contracting human herpesvirus 8? | 2000 | 10686015 | |
| occurrence of primary cancers in association with multiple myeloma and kaposi's sarcoma in the united states, 1973-1995. | the causes of multiple myeloma (mm) are obscure, but a laboratory association was recently reported between mm and human herpesvirus 8 (hhv-8), the probable etiologic agent of kaposi's sarcoma (ks). although there has been some additional laboratory corroboration, most laboratory studies have found no association between mm and hhv-8. we looked for indirect evidence of an hhv-8/mm association by evaluating whether mm is associated with ks in the united states. cancer incidence and survival data ... | 2000 | 10699913 |
| the primary sequence of rhesus monkey rhadinovirus isolate 26-95: sequence similarities to kaposi's sarcoma-associated herpesvirus and rhesus monkey rhadinovirus isolate 17577. | the primary sequence of the long unique region l-dna (l for low gc) of rhesus monkey rhadinovirus (rrv) isolate 26-95 was determined. the l-dna consists of 130,733 bp that contain 84 open reading frames (orfs). the overall organization of the rrv26-95 genome was found to be very similar to that of human kaposi sarcoma-associated herpesvirus (kshv). blast search analysis revealed that in almost all cases rrv26-95 coding sequences have a greater degree of similarity to corresponding kshv sequences ... | 2000 | 10708456 |
| changing prevalence of oral manifestations of human immuno-deficiency virus in the era of protease inhibitor therapy. | the purpose of this study was to determine temporal trends in the prevalence of oral manifestations of human immunodeficiency virus (hiv). | 2000 | 10710453 |
| serologic prevalence of antibody to human herpesvirus type 8 in patients with various monoclonal gammopathies. | both viral and serologic studies have consistently shown an association of human herpesvirus type 8 (hhv-8) with kaposi's sarcoma, primary effusion lymphoma, and castleman's disease. the presence of hhv-8 dna in patients with myeloma has been reported by some investigators but not substantiated by others. in addition, variable results have been obtained with serologic studies for hhv-8 in patients with myeloma and certain other monoclonal gammopathies (mg). we tested 238 coded serum or plasma sa ... | 2000 | 10721787 |
| kaposi's sarcoma and transplantation. | kaposi's sarcoma is a tumour of multicentric origin, composed of endothelium-lined vascular spaces and spindle-shaped cells. the incidence of kaposi's sarcoma in transplant recipients is 400--500 times greater than that in the general population, and is rising within the transplant population, currently comprising more than 5% of all de novo neoplasms in this group. the exact pathogenesis is still unknown but dna sequences from human herpesvirus 8 (hhv-8) are present in the different clinical va ... | 2000 | 11866996 |
| human herpesvirus 8 and protection from aids dementia complex. | infection with human herpesvirus 8 (hhv-8) has been associated with the development of three distinct conditions: kaposi's sarcoma, body cavitybased lymphoma and castleman's disease. hhv-8 produces chemokinelike proteins including viral macrophage inflammatory protein ii, which has been shown to block human immunodeficiency virus 1 (hiv-1) infection of cd4-positive cells expressing ccr-3. as ccr-3 is a receptor for hiv-1 into microglial cells, it has been hypothesized that hhv-8 infection may in ... | 2000 | 11867000 |
| [gastric epithelioid angiosarcoma, a biopsy diagnostic pitfall]. | we report the case of a 70-year-old man who presented a bulky gastric mass. the diagnosis of poorly differenciated adenocarcinoma was made on the biopsy. on surgical specimen, the final diagnosis of gastric angiosarcoma with secondary intestinal involvement was established. the patient did not respond to chemotherapy and died 8 months later. the diagnosis was difficult because of epithelioid areas which expressed both cytokeratin and vascular antigens. human herpesvirus 8 previously detected in ... | 2001 | 11852364 |
| [etiopathogenic importance of human herpes viruses type 6, 7 and 8 in manifestations of certain skin diseases]. | introduction: in the past few years new human herpes viruses (hhv): hhv-6, -7 and -8 have been discovered. according to the most recent literature, they might have an important role in etiopathogenesis of some dermatological diseases. human herpesvirus 6: hhv-6 was isolated in 1984 from peripheral blood lymphocytes of aids patients and patients with different lymphoproliferative diseases. up to now, two variants of this virus have been identified, a and b, which differ in genetic, biological and ... | 2001 | 11876008 |
| the kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen binds to specific sequences at the left end of the viral genome through its carboxy-terminus. | latent infection by members of the gammaherpesvirus family is typically characterized by stable episomal maintenance of genomic viral dna. in the case of epstein--barr virus (ebv), this is dependent upon binding of the epstein-barr nuclear antigen 1 (ebna1) to sites which lie within the origin of plasmid replication (orip). the recently discovered kaposi's sarcoma-associated herpesvirus (kshv) encodes the latency-associated nuclear antigen (lana), which appears to be important for supporting the ... | 2001 | 11878894 |
| two cases of kaposi's sarcoma mimicking stewart-treves syndrome found to be human herpesvirus-8 positive. | although angiosarcoma is the most frequent tumor arising in the clinical setting of chronic lymphedema, as in stewart-treves syndrome, kaposi's sarcoma has also been reported in this setting, although rarely. we describe two women who developed kaposi's sarcoma in the lymphedematous arm many years after surgery for breast cancer. case 1 is a 92-year-old and case 2 is an 81-year-old; they underwent left total mastectomy and axillary node dissection for infiltrating breast carcinoma in 1981 and 19 ... | 2001 | 11801776 |
| a seroprevalence study of human herpesvirus type 8 (hhv8) in eastern and central africa and in the mediterranean area. | human herpes virus type 8 (hhv8) is the major determinant of kaposi's sarcoma (ks), a neoplasm with wide geographic variations in incidence rates. to assess the prevalence of hhv8 infection among populations with differing rates of ks, we used sera from 1,402 persons (central africa: cameroon, n = 293, age range: 5-40; eastern africa: uganda, n = 315, age range: 1-64: mediterranean area: egypt, n = 236, age range: 13-19: italy, blood donors n = 134, age range: 20-67: italy. hiv seroconverters n ... | 2001 | 12081107 |
| emerging viral infections. | the past decade has witnessed the emergence of several significant viral pathogens and the further evolution of additional viral pathogens. transmitted by a variety of differing routes, these organisms have presented substantial intellectual challenges to medicine of the 20th and 21st centuries. as perhaps the benchmark pathogen of the past decade, hiv has provided medicine and society with a most formidable opponent, and one that has yet to be fully conquered. nonetheless, a variety of addition ... | 2001 | 11964867 |
| skin diseases associated with human herpesvirus 6, 7, and 8 infection. | relatively recently, the discovery and analysis of three new human herpesviruses, human herpesvirus (hhv)-6, hhv-7, and kaposi's sarcoma-associated herpesvirus (kshv), also known as hhv-8, has contributed greatly to our understanding of the pathogenesis of several common dermatoses. hhv-6 and hhv-7 are closely related beta-herpesviruses that have been linked with roseola (mostly hhv-6), severe drug eruptions (hhv-6), and pityriasis rosea (mostly hhv-7). kshv is a gamma-herpesvirus that is now be ... | 2001 | 11924827 |
| constitutive signaling of the human cytomegalovirus-encoded chemokine receptor us28. | previously it was shown that the hhv-8-encoded chemokine receptor orf74 shows considerable agonist-independent, constitutive activity giving rise to oncogenic transformation (arvanitakis, l., geras-raaka, e., varma, a., gershengorn, m. c., and cesarman, e. (1997) nature 385, 347-350). in this study we report that a second viral-encoded chemokine receptor, the human cytomegalovirus-encoded us28, also efficiently signals in an agonist-independent manner. transient expression of us28 in cos-7 cells ... | 2001 | 11050102 |
| high incidence of classic kaposi's sarcoma in mantua, po valley, northern italy (1989-1998). | the incidence of classic kaposi's sarcoma was estimated in the province of mantua, po valley, northern italy, yielding age-standardized rates of 2.5/100 000 men and 0.7/100 000 women (1989-98). elevated rates in the rural zone of viadana/sabbioneta (5.0/100 000 men and 2.8/100 000 women) are among the highest so far reported for italian communities. | 2001 | 11487269 |
| classic kaposi's sarcoma associated with human herpesvirus 8 infection in a 13-year-old male: a case report. | classic kaposi's sarcoma (ks) is rare in children. although its etiology is not fully understood, human herpesvirus 8 (hhv-8) is present in the angiogenic lesions. we report an hiv-negative, 13-year-old patient of sicilian descent with hhv-8-associated classic ks to facilitate the diagnosis and treatment of this entity in children. | 2001 | 11489800 |
| emerging viruses and transfusion. | the development of new technologies leads to the discovery of new viruses. for each of these new infectious agents, their possible relevance to blood transfusion needs to be assessed. the questions to be answered are transmissibility by transfusion, pathogenicity, prevalence in blood donors, persistence, and the availability of screening assays. since 1995, three new viruses have been identified and extensively studied. | 2001 | 11499963 |
| expression of kaposi's sarcoma-associated herpesvirus g protein-coupled receptor monocistronic and bicistronic transcripts in primary effusion lymphomas. | kaposi's sarcoma-associated herpesvirus (kshv/hhv-8) encodes a g protein-coupled receptor (vgpcr) in open reading frame (orf) 74, which is homologous to human chemokine receptors. kshv vgpcr is constitutively active and induces vegf-mediated angiogenesis. previous studies have shown that orf 74 is transcribed as part of a bicistronic message containing orf k14 upstream of orf 74, with an early lytic pattern of expression. we have now extended these studies by analyzing three different kshv-posit ... | 2001 | 11504542 |
| activation of nf-kappab by the human herpesvirus 8 chemokine receptor orf74: evidence for a paracrine model of kaposi's sarcoma pathogenesis. | infection with human herpesvirus 8 (hhv-8), also known as kaposi's sarcoma (ks)-associated herpesvirus, is necessary for the development of ks. the hhv-8 lytic-phase gene orf74 is related to g protein-coupled receptors, particularly interleukin-8 (il-8) receptors. orf74 activates the inositol phosphate/phospholipase c pathway and the downstream mitogen-activated protein kinases, jnk/sapk and p38. we show here that orf74 also activates nf-kappab independent of ligand when expressed in ks-derived ... | 2001 | 11507211 |
| latency-associated nuclear antigen encoded by kaposi's sarcoma-associated herpesvirus interacts with tat and activates the long terminal repeat of human immunodeficiency virus type 1 in human cells. | the latency-associated nuclear antigen (lana) is constitutively expressed in cells infected with the kaposi's sarcoma (ks) herpesvirus (kshv), also referred to as human herpesvirus 8. kshv is tightly associated with body cavity-based lymphomas (bcbls) in immunocompromised patients infected with human immunodeficiency virus (hiv). lana, encoded by open reading frame 73 of kshv, is one of a small subset of proteins expressed during latent infection and was shown to be important in tethering the vi ... | 2001 | 11507221 |
| herpesvirus type 1-8 in bal fluid from hiv-1-infected patients with suspected pneumonia and from healthy individuals. | pneumonia is still a major problem in human immunodeficiency virus (hiv)-infected patients, and despite extensive investigation the aetiology remains unknown in many cases. the prevalence of the eight human herpesviruses was determined by polymerase chain reaction in 91 samples of bronchoalveolar lavage (bal) fluid from 72 hiv-infected patients with 91 episodes of suspected pneumonia. the presence of herpesviruses was related to clinical and immunological findings and the prevalence of herpesvir ... | 2001 | 11510785 |
| safety of italian plasma for fractionation with respect to contamination with human herpes virus 8. | 2001 | 11520418 | |
| low incidence of human herpesvirus 8 in bone marrow samples from brazilian patients with multiple myeloma. | 2001 | 11528101 | |
| a novel gamma 2-herpesvirus of the rhadinovirus 2 lineage in chimpanzees. | old world monkeys and, recently, african great apes have been shown, by serology and polymerase chain reaction (pcr), to harbor different gamma2-herpesviruses closely related to kaposi's sarcoma-associated herpesvirus (kshv). although the presence of two distinct lineages of kshv-like rhadinoviruses, rv1 and rv2, has been revealed in old world primates (including african green monkeys, macaques, and, recently, mandrills), viruses belonging to the rv2 genogroup have not yet been identified from g ... | 2001 | 11544194 |
| seroprevalence of human herpesvirus 8 in okinawa, japan. | 2001 | 11544407 | |
| seroprevalence of human herpes virus 8 antibody in populations at high or low risk of transfusion, graft, or sexual transmission of viruses. | the routes of transmission of human herpes virus 8 (hhv-8) remain unclear. in particular, hhv-8 transmission by blood components and organ transplantation is still debated and raises public health issues. the objective of this study was to determine the prevalence of anti-hhv-8 in selected populations of persons or patients with or without risk factors for the transmission of viral infections, in order to determine the routes of hhv-8 transmission. | 2001 | 11552068 |
| seroprevalence of kaposi's sarcoma-associated herpesvirus infection among blood donors from texas. | kaposi's sarcoma-associated herpesvirus (kshv), a gammaherpesvirus recently discovered among aids patients with kaposi's sarcoma, is a potential candidate for screening in blood and plasma donors. while a number of studies have assessed kshv infection among u.s. blood donors, larger-scale population-based studies would be necessary to develop more refined estimates of the magnitude and variation of kshv infection across different geographic regions of the u.s. blood supply. the goal of the prese ... | 2001 | 11557184 |
| treatment of kaposi sarcoma with oral administration of shark cartilage in a human herpesvirus 8-seropositive, human immunodeficiency virus-seronegative homosexual man. | 2001 | 11559209 | |
| [human herpes virus and malignancies]. | two type of human herpes viruses, type 4(epstein barr virus, ebv) and type 8(hhv8) are known to cause malignancies. among those, ebv is the very most popular human oncovirus. it can cause malignant lymphomas of various subtypes including burkitt's lymphoma, opportunistic lymphomas, pyothorax-associated lymphoma, nasal t/nk cell lymphoma, hodgkin's disease and a part of diffuse large cell lymphomas. hhv8 is a new member of human oncovirus associating with primary effusion lymphoma, a part of soli ... | 2001 | 11573285 |
| quantitative, fluorogenic probe pcr assay for detection of human herpesvirus 8 dna in clinical specimens. | a quantitative, fluorescence-based pcr assay (taqman-based system) was developed for detection of human herpesvirus 8 (hhv-8) dna in clinical specimens. primers and probes chosen from each of five 10-kb segments from the unique region of the hhv-8 genome were evaluated for sensitivity with dilution series of dna extracted from a cell line (bcbl-1) that harbors hhv-8 dna. although several of the primer-probe sets performed similarly with bcbl-1 dna that had been diluted in water, their performanc ... | 2001 | 11574569 |