Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [helicobacter pylori--introduction and review of research]. | the discovery of helicobacter pylori has revolutionized the pathophysiological and clinical approach to gastric and duodenal ulcer. since the first paper identifying h. pylori was published only 17 years ago, it has been found out that this bacterium causes probably the commonest human infection. numerous papers published so far have confirmed causal relationship between h. pylori infection and gastritis, duodenal ulcer, gastric ulcer and gastric cancer. if any recent achievement in the world of ... | 2002 | 12592806 |
| deregulation of shp-2 tyrosine phosphatase by the helicobacter pylori virulence factor caga. | helicobacter pylori (h. pylori) is estimated to infect about half of the world population. it causes gastric diseases ranging from gastritis to cancer and has been classified as a class i carcinogen by who. however, little is known about the molecular mechanisms by which h. pylori induces pathogenesis. caga is the product of the caga gene carried among virulent h. pylori strains and is associated with severe clinical outcomes, most notably gastric carcinoma. caga is injected from the attached h. ... | 2002 | 12528933 |
| heat shock protein of mycoplasma salivarium and mycoplasma orale strains isolated from hiv-seropositive patients. | it has been suggested that infection by some mycoplasma species can act as possible cofactors in the acceleration of immunodeficiency in hiv-infected patients. the present study was designed to examine infections by oral mycoplasma species in hiv-seropositive (hiv(+)) patients. mycoplasma salivarium and mycoplasma orale were isolated from 59.5% and 16.7% of 42 hiv(+) patients, respectively. non-m. salivarium and non-m. orale species were isolated from 40.5% of saliva samples from the hiv(+) grou ... | 2002 | 12687728 |
| immunoproteomics of helicobacter pylori infection and relation to gastric disease. | the gram negative bacterium helicobacter pylori is a human pathogen which infects the gastric mucosa and causes an inflammatory process leading to gastritis, ulceration and cancer. a systematic, proteome based approach was chosen to detect candidate antigens of h. pylori for diagnosis, therapy and vaccine development and to investigate potential associations between specific immune responses and manifestations of disease. sera from patients with active h. pylori infection (n = 24), a control gro ... | 2002 | 11921447 |
| critical role of an endogenous gastric peroxidase in controlling oxidative damage in h. pylori-mediated and nonmediated gastric ulcer. | the objective of the present study is to delineate the mechanism of oxidative damage in human gastric ulcerated mucosa despite the presence of some antioxidant enzymes. we report for the first time the critical role of an endogenous peroxidase, a major h(2)o(2) metabolizing enzyme, in controlling oxidative damage in gastric mucosa. human gastric mucosa contains a highly active peroxidase in addition to the myeloperoxidase contributed by neutrophil. in both non-helicobacter pylori (h. pylori)- an ... | 2002 | 11937299 |
| helicobacter pylori infection in skin diseases: a critical appraisal. | more than 50% of the human population have long-term helicobacter pylori infection, causing, in some cases, severe diseases such as peptic ulcers and stomach cancer. in the last few years several extra-gastrointestinal disorders have been associated with h. pylori infection. this review summarized the current medical literature, identified through hand searching and medline research, including our own studies, with regard to h. pylori and skin diseases. from the literature it can be seen that th ... | 2002 | 12010072 |
| adams (a disintegrin and metalloproteinase) messenger rna expression in helicobacter pylori-infected, normal, and neoplastic gastric mucosa. | helicobacter pylori is a risk factor for gastric cancer, and bacterial-epithelial interactions may be critical in this association. studies using complementary dna arrays indicated that the adam (a disintegrin and metalloproteinase) genes in gastric epithelial cells are differentially expressed after bacterial-epithelial interactions. reverse-transcription polymerase chain reaction analysis of gastric biopsy specimens from patients with and without h. pylori showed that infection was associated ... | 2002 | 11807715 |
| identification of helicobacter pylori dna in human cholesterol gallstones. | the gallbladder mucosa secretes hydrogen ions and is covered by mucus. the environmental conditions for bacterial colonization are similar to those in the stomach. gallbladder stones often contain dna from enteric bacteria, but no compelling evidence demonstrates that helicobacter spp. have been present. the aim of this study was to establish bacterial dna profiles in cholesterol gallstones with special reference to helicobacter pylori. | 2002 | 11843027 |
| association analysis of polymorphisms at the interleukin-1 locus in essential hypertension. | infection with microorganisms such as helicobacter pylori and chlamydia pneumoniae has been associated with coronary heart disease (cad) and hypertension (ht). infection increases the release of pro-inflammatory cytokines, thus facilitating interactions that lead to vascular damage and other effects. we hypothesized that genetically determined differences in activity or responsiveness of cytokine(s) might contribute to ht. the interleukin-1 gene (il1) cluster on chromosome 2q14 contains three re ... | 2002 | 11840488 |
| effects of n-alpha-methyl-histamine on human h(2) receptors expressed in cho cells. | production of n-alpha-methyl-histamine (namh), a histamine h(3) receptor (h3r) agonist, is reportedly promoted in helicobacter pylori infected human gastric mucosa. namh was suggested to act directly on histamine h(2) receptors (h2rs) in animals to stimulate acid secretion and to be a h2r agonist. as h2rs and h3rs play different roles in gastric acid secretion, it is very important to verify that namh is a h2r agonist. | 2002 | 12010879 |
| dip, the database of interacting proteins: a research tool for studying cellular networks of protein interactions. | the database of interacting proteins (dip: http://dip.doe-mbi.ucla.edu) is a database that documents experimentally determined protein-protein interactions. it provides the scientific community with an integrated set of tools for browsing and extracting information about protein interaction networks. as of september 2001, the dip catalogs approximately 11 000 unique interactions among 5900 proteins from >80 organisms; the vast majority from yeast, helicobacter pylori and human. tools have been d ... | 2002 | 11752321 |
| removal of mucus for ultrastructural observation of the surface of human gastric epithelium using pronase. | helicobacter pylori adhering to the human gastric epithelium causes gastric diseases such as ulcer, carcinoma and lymphoma. it is thus important to observe in detail both the surface of the epithelial cells and the h. pylori that adhered to it for the elucidation of h. pylori-induced diseases by scanning electron microscopy (sem). since the thick mucus layer blocks the observation of the cell surface and the bacteria, it is generally eliminated during the processing for sem by roughly mechanical ... | 2002 | 11966871 |
| shp-2 tyrosine phosphatase as an intracellular target of helicobacter pylori caga protein. | helicobacter pylori caga protein is associated with severe gastritis and gastric carcinoma. caga is injected from the attached helicobacter pylori into host cells and undergoes tyrosine phosphorylation. wild-type but not phosphorylation-resistant caga induced a growth factor-like response in gastric epithelial cells. furthermore, caga formed a physical complex with the src homology 2 domain (sh2)-containing tyrosine phosphatase shp-2 in a phosphorylation-dependent manner and stimulated the phosp ... | 2002 | 11743164 |
| [gastric epithelial cell apoptosis and il-18]. | 2002 | 11979769 | |
| src is the kinase of the helicobacter pylori caga protein in vitro and in vivo. | the gastric pathogen helicobacter pylori uses a type iv secretion system to inject the bacterial caga protein into gastric epithelial cells. within the host cell, caga becomes phosphorylated on tyrosine residues and initiates cytoskeletal rearrangements. we demonstrate here that src-like protein-tyrosine kinases mediate caga phosphorylation in vitro and in vivo. first, the src-specific tyrosine kinase inhibitor pp2 specifically blocks caga phosphorylation and cytoskeletal rearrangements thereby ... | 2002 | 11788577 |
| bacterial secrets of secretion: euroconference on the biology of type iv secretion processes. | type iv secretion systems (tfss) mediate secretion or direct cell-to-cell transfer of virulence factors (proteins or protein-dna complexes) from many gram-negative animal, human and plant pathogens, such as agrobacterium tumefaciens, bartonella tribocorum, bordetella pertussis, brucella suis, helicobacter pylori, legionella pneumophila and rickettsia prowazekii, into eukaryotic cells. bacterial conjugation is also classified as a tfss-like process mediating the spread of broad-host-range plasmid ... | 2002 | 11918819 |
| effect of cold starvation, acid stress, and nutrients on metabolic activity of helicobacter pylori. | helicobacter pylori can transform, in vivo as well as in vitro, from dividing spiral-shaped forms into nonculturable coccoids, with intermediate forms called u forms. the importance of nonculturable coccoid forms of h. pylori in disease transmission and antibiotic treatment failures is unclear. metabolic activities of actively growing as well as nonculturable h. pylori were investigated by comparing the concentrations of cellular atp and total rna, gene expression, presence of cytoplasmic polyph ... | 2002 | 11772603 |
| helicobacter pylori stimulates pepsinogen secretion from isolated human peptic cells. | different acid and peptic related gastroduodenal diseases are associated with both increased gastric secretion and helicobacter pylori infection. patients with h pylori associated gastritis or duodenal ulcer have increased serum pepsinogen levels which decrease after eradication. the mechanisms of h pylori induced gastric mucosal damage are not completely understood. | 2002 | 11772960 |
| human plasma is directly bacteriocidal against helicobacter pylori in vitro, potentially explaining the decreased detection of helicobacter pylori during acute upper gi bleeding. | the rapid urease test is the preferred method for detection of h pylori in patients with uncomplicated ulcer disease undergoing egd. however, the sensitivity of this test when performed during upper gi bleeding has been questioned. it has been suggested that false-negative results occur, resulting from buffering effects of blood. the direct effect of blood was evaluated on h pylori growth in ex vivo and in vitro systems. | 2002 | 11756907 |
| reversibility of heterotopic proliferative glands in glandular stomach of helicobacter pylori-infected mongolian gerbils on eradication. | helicobacter pylori (hp) infection is an important factor in human gastric disorders. mongolian gerbils can be easily infected with hp and represent excellent experimental models to clarify the role of hp in chronic active gastritis, peptic ulcers, intestinal metaplasia, and gastric carcinoma. we have proved the enhancing effects of hp infection on all histological types of gastric cancers in mongolian gerbils exposed to chemical carcinogens. heterotopic proliferative glands (hpgs) also frequent ... | 2002 | 11985786 |
| helicobacter pylori activates the proto-oncogene c-fos through sre transactivation. | epidemiological studies have demonstrated a strong association between helicobacter pylori infection and gastric cancer. however, there have been few detailed studies on the mechanism of cellular proliferation by h. pylori. thus, we examined activation of the proto-oncogene c-fos to elucidate the underlying mechanism of cell proliferation caused by h. pylori. activation of c-fos was evaluated in human gastric cancer cells (tmk1) by northern blot and reporter assays with deletion analysis of the ... | 2002 | 11866445 |
| extracellular ph modulates helicobacter pylori-induced vacuolation and vaca toxin internalization in human gastric epithelial cells. | in this study we investigated whether an acidic extracellular ph may inhibit h. pylori-induced internalization of bacterial virulence factors by gastric epithelium, thus preventing ingestion of potentially dangerous luminal contents and resulting cellular damage. the interaction of h. pylori vaca toxin and ammonia (produced by h. pylori urease) with partly polarized gastric mkn 28 cells in culture was investigated at neutral and moderately acidic ph (6.2, compatible with cell viability) by means ... | 2002 | 11890688 |
| effect of lactoferrin on helicobacter felis induced gastritis. | lactoferrin possesses antibiotic, antiinflammatory, and immune-modulating properties that may be active against the gastritis-, ulcer- and cancer-inducing bacterium helicobacter pylori. in vitro testing of bovine and human lactoferrin by several laboratories has shown significant bacteriostatic and bactericidal activity. subsequent in vivo testing of bovine lactoferrin in animal models of h. pylori infection has shown beneficial effects of this agent. our laboratory has utilized a mouse model th ... | 2002 | 11908634 |
| diagnosis of a gastric mucosa-associated lymphoid tissue lymphoma by endoscopic ultrasonography-guided biopsies in a patient with a parotid gland localization. | we report the case of a 32-year-old man with a low-grade mucosa-associated lymphoid tissue (malt) lymphoma of the parotid gland associated with sjögren syndrome. he underwent an upper endoscopy as part of the screening of a gastric localization which showed a diffuse non-specific gastritis. however, endoscopic ultrasonography (eus) evidenced a focal wall thickening of the vertical portion of the smaller curvature. eus-guided biopsies of this area disclosed a malt lymphoma, whereas biopsies under ... | 2002 | 11989843 |
| helicobacter pylori infection and infertility. | to determine (1) the prevalence of helicobacter pylori infection in male and female patients with reproductive disorders and controls; (2) the presence of anti-h. pylori antibodies in samples of follicular fluid, vaginal secretions and sperm; and (3) the existence of a structural homology between a major spermatozoa protein, tubulin, and h. pylori proteins. | 2002 | 12072601 |
| review article: natural history and epidemiology of helicobacter pylori infection. | helicobacter pylori is a common bacterium infecting about half the world's population. it is causally linked with a diverse spectrum of gastrointestinal clinical disorders including peptic ulcer disease, gastric cancer, and gastric malt lymphoma. the principal reservoir is the human stomach, and transmission probably occurs by person-to-person passage. prevalence rates are generally much higher in developing countries compared to developed countries, although there are subgroups within many regi ... | 2002 | 11849122 |
| helicobacter pylori infection in rural china: demographic, lifestyle and environmental factors. | although helicobacter pylori is one of the most common human bacterial infections worldwide, its mode of transmission is unclear. | 2002 | 12055167 |
| adherence of helicobacter pylori to gastric epithelial cells and mucosal inflammation. | adherence of helicobacter pylori to the gastric epithelium is believed to be an important step in the induction of active inflammation of the mucosal layer. however, structural evidence showing a quantitative relationship between the adherence of h. pylori and severity of gastric mucosal inflammation is lacking. we therefore investigated the correlations between severity of gastritis and adherence of morphologically different forms of h. pylori. fifty-seven biopsy specimens from the gastric bodi ... | 2002 | 12024112 |
| helicobacter pylori-induced activation of human endothelial cells. | helicobacter pylori infection causes active chronic inflammation with a continuous recruitment of neutrophils to the inflamed gastric mucosa. to evaluate the role of endothelial cells in this process, we have examined adhesion molecule expression and chemokine and cytokine production from human umbilical vein endothelial cells stimulated with well-characterized h. pylori strains as well as purified proteins. our results indicate that endothelial cells actively contribute to neutrophil recruitmen ... | 2002 | 12117971 |
| differential glycosylation of mhc class ii molecules on gastric epithelial cells: implications in local immune responses. | class ii major histocompatibility complex (mhc) expression is a hallmark of antigen presenting cells (apc). human gastric epithelial cells (gec) express class ii mhc and this expression increases during infection with helicobacter pylori as does the number of cd4 t cells found adjacent or in between epithelial cells. these observations suggested that human gec act as apcs. to characterize and compare class ii mhc complexes with those present in conventional apc, immunoprecipitated class ii mhc f ... | 2002 | 11975982 |
| interrelation between abh blood group 0, lewis(b) blood group antigen, helicobacter pylori infection, and occurrence of peptic ulcer. | helicobacter pylori is a human pathogen that causes chronic gastritis and peptic ulcers. epidemiological studies demonstrated that individuals who are blood group 0 positive or represent non-secretors of their blood group antigens are more likely to develop peptic ulcers. the lewis(b) blood group antigen has been reported to mediate the attachment of h. pylori to human gastric mucosa. the aim of this study was to examine the interrelation between le(a-b+) phenotype, blood group 0, h. pylori infe ... | 2002 | 12016560 |
| identification of surface proteins of helicobacter pylori by selective biotinylation, affinity purification, and two-dimensional gel electrophoresis. | helicobacter pylori is a widespread human pathogen that can cause gastric ulcers and cancer. to identify surface proteins that may play a role in pathogen-host interactions and represent potential targets for the control of this infection, we selectively biotinylated intact h. pylori with the hydrophilic reagent sulfosuccinimidyl-6-(biotinamido)-hexanoate and purified the labeled proteins by membrane isolation, solubilization, and affinity chromatography. after separation of 82 biotinylated prot ... | 2002 | 12023975 |
| [appearance of helicobacter pylori antigen in stool specimens of children with chronic gastritis]. | various examinations are successfully applied for the diagnosis of helicobacter pylori infection, but non-invasive techniques are still required for the therapeutic monitoring after eradication therapy, especially in children. in the search for new non-invasive techniques to diagnose h. pylori infection, we evaluated an eia for h. pylori antigen in stool (hpsa). our study included 62 h. pylori-positive children with chronic gastritis. h. pylori infection was diagnosed by using the culture of gas ... | 2002 | 11995244 |
| proteome analysis of highly immunoreactive proteins of helicobacter pylori. | identification of the immunoreactive proteins of helicobacter pylori is important for the development of both diagnostic tests and vaccines relating to the organism. our aim was to determine whether there are significant differences between human igg and iga reactivities to individual h. pylori proteins, and whether patterns of immunoreactivity are sustained across different strains of h. pylori. | 2002 | 12047323 |
| microaerophilic conditions permit to mimic in vitro events occurring during in vivo helicobacter pylori infection and to identify rho/ras-associated proteins in cellular signaling. | molecular dissection of the mechanisms underlying helicobacter pylori infection suffers from the lack of in vitro systems mimicking in vivo observations. a system was developed whereby human epithelial cells (caco-2) grown as polarized monolayers and bacteria can communicate with each other under culture conditions optimal for each partner. caco-2 cells grown on filter supports were inserted in a vertical position into diffusion chambers equilibrated with air and 5% co(2) at their basolateral su ... | 2002 | 12058029 |
| helicobacter pylori-induced oxidative stress and dna damage in a primary culture of human gastric mucosal cells. | helicobacter pylori has been identified in the pathogenesis of chronic active gastritis and peptic ulcer disease and is epidemiologically linked to gastric cancer and lymphoma. our previous studies have demonstrated enhanced production of reactive oxygen species (ros) in cultured gastric adenocarcinoma cells (atcc crl/1739) in association with h. pylori. recently, we have isolated and cultured normal human gastric mucosal cells (gmc) from h. pylori-negative endoscopic biopsies. the integrity of ... | 2002 | 12064819 |
| effect of weissella confusa strain pl9001 on the adherence and growth of helicobacter pylori. | viable and nonviable weissella confusa strain pl9001 inhibited the binding of helicobacter pylori to human gastric-cell line mkn-45 cells by more than 90%. spent culture supernatant of pl9001 rapidly decreased the viability of h. pylori, rupturing cell walls. the results suggest that pl9001 is a probiotic that can reduce the infectivity and persistence of h. pylori. | 2002 | 12200324 |
| inhibition of cell proliferation and induction of apoptosis by helicobacter pylori through increased phosphorylated p53, p21 and bax expression in endothelial cells. | microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. therefore, the direct effect of helicobacter pylori on endothelial cells in vitro was investigated with h. pylori water extract. the effect of h. pylori water extract on cell proliferation and apoptosis of human umbilical vein endothelial cells (huvecs) was evaluated. the ratio of brdu-positive ... | 2002 | 11990490 |
| expression of g1 phase cyclins in human gastric cancer and gastric mucosa of first-degree relatives. | the aim of this study was to investigate the expression of d-type cyclins and cyclin e in gastric cancer patients (n = 34), in healthy first-degree relatives of gastric cancer patients (n = 29), and in control subjects (n = 18). expression of cyclins d1, d2, d3, and e was determined by rt-pcr. localization of cyclin expression was determined by immunohistochemistry. expression of cyclins d2, d3, and e was more frequently detected in tumor tissue compared with tumor-free gastric mucosa (p < 0.05) ... | 2002 | 12064799 |
| hp (2-20) derived from the amino terminal region of helicobacterpylori ribosomal protein l1 exerts its antifungal effects by damaging the plasma membranes of candida albicans. | the fungicidal effects of the peptide hp (2-20). derived from the n-terminal sequence of helicobacter pylori ribosomal protein l1 (rpl1). have been investigated. hp (2-20) displays a strong fungicidal activity against various fungi, without haemolytic activity against human erythrocyte cells, and the fungicidal activity is inhibited by ca2+ and mg2+ ions. in order to investigate the fungicidal mechanism(s) of hp (2-20). the amount of intracellular trehalose was measured in c. albicans. it was fo ... | 2002 | 12212808 |
| expression of mucins (muc1, muc2, muc5ac, and muc6) and type 1 lewis antigens in cases with and without helicobacter pylori colonization in metaplastic glands of the human stomach. | helicobacter pylori (h. pylori) causes gastritis and intestinal metaplasia (tm) that may evolve to gastric carcinoma. paradoxically, im leads to clearing of h. pylori, except for some cases in which it persists in damaging the mucosa. the objective of this study was to compare the profile of mucins and type 1 lewis antigens in im cases with and without h. pylori. gastric biopsies (n=32) were double-stained using immunohistochemistry (muc1, muc2, muc5ac, muc6, le(a), sialyl-le(a), and le(b)) and ... | 2002 | 12081201 |
| increased in-vitro and in-vivo biological activity of lipopolysaccharide extracted from clinical low virulence vaca genotype helicobacter pylori strains. | helicobacter pylori infection in man is associated with chronic gastritis and peptic ulcer disease. the virulence factors of the species are still under investigation. among these, the lipopolysaccharide (lps) is a potential pathogenic factor of the micro-organism, whose biological activity can be estimated by immunological parameters. the aim of this study was to determine the ability of pure lps extracted from clinical isolates of h. pylori to induce mitogenicity, secretion of tumour necrosis ... | 2002 | 12358068 |
| correlation between helicobacter pylori infection and il-18 mrna expression in human gastric biopsy specimens. | our data indicate that h. pylori infection is associated with active interleukin-18 production in patients with chronic gastritis. different cell types appear to be involved in this activity and may play a role in the development of immunopathologic damage. | 2002 | 12095959 |
| interleukin 1beta polymorphisms increase risk of hypochlorhydria and atrophic gastritis and reduce risk of duodenal ulcer recurrence in japan. | interleukin-1 beta (il-1beta) polymorphisms are associated with increased risk of gastric cancer in whites. this study aimed to examine effects of these polymorphisms on gastric acid secretion, atrophic gastritis, and risk of peptic ulcer in japan. | 2002 | 12105837 |
| analysis of p53 mutations and helicobacter pylori infection in human and animal models. | p53 gene mutations are believed to play a critical role in the development of gastric carcinoma. we examined the relation between helicobacter pylori infection and p53 gene mutations of the gastric mucosa in human and animal models. | 2002 | 12109656 |
| involvement of tnf-alpha changes in human cancer development, prevention and palliative care. | cancer development and ageing are complex sciences. from the study on the process of rodent carcinogenesis, we identified tumor necrosis factor-alpha (tnf-alpha) as an important mediator of cancer development. this paper presents three clinical examples of tnf-alpha up-regulation: by cord factors of mycobacterium tuberculosis, such as trehalose 6-monomycolate, as an activator of protein kinase c and by a cord factor like fraction of microsporum canis obtained in the air inside houses in thailand ... | 2002 | 12470903 |
| randomized open trial for comparison of proton pump inhibitors in triple therapy for helicobacter pylori infection in relation to cyp2c19 genotype. | genetic polymorphism of cytochrome p450 (cyp) 2c19 influences the efficacy of helicobacter pylori eradication therapy with a proton pump inhibitor (ppi) and amoxicillin. however, in triple therapy (ppi plus amoxicillin and clarithromycin), little is known about the impact of cyp2c19 polymorphism, or the use of rabeprazole, which is not well metabolized by cyp2c19. the efficacy of three ppi (omeprazole, lansoprazole, and rabeprazole) in a 1-week triple regimen were compared in relation to cyp2c19 ... | 2002 | 12121503 |
| [association between helicobacter pylori caga strain infection and expression of cyclooxygenase 2 in gastric carcinoma]. | to observe the expression of cyclooxygenase 2 (cox-2) in tissues of human gastric cancer and explore the association between helicobacter pylori caga strain infection and the expression of cox-2 so as to provide a theoretical basis for early prevention of gastric cancer. | 2002 | 12126507 |
| identification of novel immunogenic proteins of helicobacter pylori by proteome technology. | cell surface proteins of the human gastric pathogen helicobacter pylori, reference strain ccug 17874, were extracted with acid glycine and fractionated by heparin affinity chromatography. the extracts were subsequently analysed using high-resolution two-dimensional gel electrophoresis (2-de) and immunoblotting. four proteins of low molecular masses (25-30 kda) stained by coomassie r-350, were identified by peptide esi-ms/ms sequencing after in-gel tryptic digestion. the identified proteins were ... | 2002 | 11730836 |
| human beta-defensin-2 induction in helicobacter pylori-infected gastric mucosal tissues: antimicrobial effect of overexpression. | the objective of this study was to understand more of the innate immune response to helicobacter pylori by determining the expression of human beta-defensin-2 (hbd-2) in various gastric mucosal tissues and mkn45 gastric cancer cells with or without h. pylori. semi-quantitative taqman rt-pcr and immunohistochemistry were carried out. the antimicrobial effects of a transfected hbd-2 gene against h. pylori were also evaluated. the results showed that hbd-2 was expressed in inflamed gastric mucosal ... | 2003 | 12488564 |
| gastric stump lymphoma five years after distal gastrectomy. | we report the case of a 77-year-old man who developed low grade b cell non-hodgkin's lymphoma of the gastric stump 5 years after undergoing a distal gastrectomy for benign gastric ulcer. lymphoma occurring in the post-operative stomach would appear to be very rare, with only 14 previously recorded cases. the median period of lymphoma onset after ulcer surgery is about 20 years (range 9-43 years) and gastric remnants of lymphoma are generally diagnosed in low stage, when surgery is possible and m ... | 2003 | 12688360 |
| limited role of lipopolysaccharide lewis antigens in adherence of helicobacter pylori to the human gastric epithelium. | in vitro and in vivo studies from various groups have suggested that helicobacter pylori lipopolysaccharide (lps) lewis x (le(x)) antigens mediate bacterial adhesion. we have now reevaluated this hypothesis by studying the adherence in situ of h. pylori strain 11637 and its corresponding le(x)-negative rfbm mutant to human gastric mucosa from patients (n = 22) with various gastric pathologies. significant binding of the parent strain was observed in only 8 out of 22 sections; in four out of eigh ... | 2003 | 12704161 |
| cytoskeletal rearrangements in gastric epithelial cells in response to helicobacter pylori infection. | helicobacter pylori causes host epithelial cell cytoskeletal rearrangements mediated by the translocation and tyrosine phosphorylation of an outer-membrane protein, caga, and by the vacuolating cytotoxin, vaca. however, the mechanisms by which h. pylori mediates cytoskeletal rearrangements in infected host cells need to be more clearly defined. the aim of this study was to determine the effects of h. pylori isolates from children on the architecture of host gastric epithelial cells. gastric epit ... | 2003 | 12972579 |
| helicobacter pylori water soluble surface proteins prime human neutrophils for enhanced production of reactive oxygen species and stimulate chemokine production. | chronic gastritis induced by helicobacter pylori is characterised by considerable neutrophil infiltration into the gastric mucosa without mucosal invasion of bacteria. bacteria have different characteristics with respect to their ability to stimulate human neutrophils to produce reactive oxygen species and chemokines. the aim of this study was to examine the effects of h pylori water extracts on the oxidative burst and chemokine production of human neutrophils. | 2003 | 12719454 |
| cytotoxic and multidrug resistance reversal activity of a vegetable, 'anastasia red', a variety of sweet pepper. | the vegetable, anastasia red, capsicum annuum l. var. angulosum mill. (solanaceae) was successively extracted with hexane, acetone, methanol and 70% methanol, and the extracts were further separated into a total of 21 fractions by silica gel or octadecylsilane (ods) column chromatography. the biological activities of extracts and fractions were determined. these extracts showed relatively higher cytotoxic activity against two human oral tumor cell lines (hsc-2, hsg) than against normal human gin ... | 2003 | 12722138 |
| strategies for the enrichment and identification of basic proteins in proteome projects. | two-dimensional gel electrophoresis (2-de) is currently the method of choice for separating complex mixtures of proteins for visual comparison in proteome analysis. this technology, however, is biased against certain classes of proteins including low abundance and hydrophobic proteins. proteins with extremely alkaline isoelectric points (pi) are often very poorly represented using 2-de technology, even when complex mixtures are separated using commercially available ph 6-11 or ph 7-10 immobilize ... | 2003 | 12748937 |
| helicobacter pylori infection affects the expression of pcna, p53, c-erbb-2 and bcl-2 in the human gastric mucosa. | helicobacter pylori infection has been related to gastric carcinogenesis. this association is based on epidemiological data, pathological changes observed in the gastric mucosa, and chemical products from bacteria that may induce damage of dna. in the present study we examined gastric endoscopic biopsies from patients with chronic gastritis, with and without h. pylori infection, and surgical biopsies from gastric cancer patients to evaluate whether this bacteria may induce changes in the express ... | 2003 | 12760717 |
| helicobacter pylori activates toll-like receptor 4 expression in gastrointestinal epithelial cells. | helicobacter pylori activates the transcription factor nf-kappab, leading to proinflammatory cytokine production by gastric epithelial cells. however, the receptors for the initial bacterial interaction with host cells which activate downstream signaling events have not been completely defined. recently, it has been shown that microbial components activate toll-like receptors (tlrs), thereby leading to ap-1- and nf-kappab-dependent transcription and resulting in the production of proinflammatory ... | 2003 | 12761134 |
| disruption of the epithelial apical-junctional complex by helicobacter pylori caga. | helicobacter pylori translocates the protein caga into gastric epithelial cells and has been linked to peptic ulcer disease and gastric carcinoma. we show that injected caga associates with the epithelial tight-junction scaffolding protein zo-1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight-junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex. long-term caga delivery to p ... | 2003 | 12775840 |
| trailing gastrointestinal pathogenesis. | 2003 | 12795744 | |
| effect of total secondary carotenoids extracts from chlorococcum sp on helicobacter pylori-infected balb/c mice. | helicobacter pylori is a human pathogen associated with type b gastritis, peptic ulcer disease and gastric cancer. a high intake of carotenoids has been suggested to prevent development of gastric malignancies. microalgae chlorococcum sp. could accumulate secondary carotenoids under stress conditions. the aim of the present study was to investigate whether dietary cell extract of chlorococcum sp. could affect the bacterial load of h. pylori infected balb/c mice and whether it could induce modula ... | 2003 | 12810355 |
| expression of cd3 epsilon subunit in gastric parietal cells: a possible role in signal transduction? | cd3 antigen, formerly thought to be specific for t lymphocytes, has been found on gastric parietal cells in animals and humans. the common anti-cd3 antibodies recognize the epsilon subunit, which has a role in signal transduction. the aim of this study was to immunostain stomach specimens from humans and different animal species for cd3 antigen to determine if cd3 antigen is conserved across species and if cd3 antigen expression is altered in humans by use of certain drugs or the presence of gas ... | 2003 | 12812314 |
| priming and expression of immune responses in the gastric mucosa. | this review deals with the induction and expression of immune responses in the human stomach following mucosal immunisation. as prerequisites for developing a helicobacter pylori vaccine, the role of gastric inflammation, in particular inflammation induced by h. pylori infection, as well as lymphocyte homing within the common mucosal immune system, and the importance of immunisation route are discussed. | 2003 | 12814774 |
| helicobacter pylori: pathogen and symbiont. | there are puzzles in defining the exact role of helicobacter pylori infection in humans. the bacterium, which is associated with human disease of the upper gastrointestinal tract, may otherwise exist as a commensal with probable symbiotic association in some human hosts. although virulence associated genes have been detected in some strains which explain their pathogenicity, their pathogenic effect and subsequent clinical manifestations seem to vary in different human populations or geographical ... | 2003 | 14567051 |
| [disturbance of intracellular signaling by helicobacter pylori infection]. | 2003 | 14577356 | |
| remission of severe anemia persisting for over 20 years after eradication of helicobacter pylori in cases of ménètrier's disease and atrophic gastritis: helicobacter pylori as a pathogenic factor in iron-deficiency anemia. | a man with a 20-year history of recurrent iron-deficiency anemia complicated by helicobacter pylori-positive ménètrier's disease was observed over a 10-year clinical course, during which time he was successfully treated for the anemia and a gastric helicobacter pylori (h. pylori) infection through eradication. considering the satisfactory therapeutic results in this case, we performed eradication therapy on another h. pylori-positive atrophic gastritis case with a 24-year history of iron-deficie ... | 2003 | 14606710 |
| animal models of helicobacter pylori infection and disease. | the acceptance of helicobacter pylori as a major human pathogen has necessitated the development of animal models to help elucidate the pathogenic mechanisms of this bacterium and aid in the development of improved strategies for the treatment of gastric disease. appropriate models, utilising a range of animal species, have been developed to examine factors such as the influence of host responses and bacterial factors in disease development and the success of new therapeutic regimens, including ... | 2003 | 12814775 |
| time-dependent amplified pharmacokinetic and pharmacodynamic responses of rabeprazole in cytochrome p450 2c19 poor metabolizers. | to determine the pharmacokinetic and pharmacodynamic rationale for the optimum regimen of rabeprazole in the treatment of helicobacter pylori infection in patients who are cytochrome p450 (cyp) 2c19 poor metabolizers or extensive metabolizers. | 2003 | 12820812 |
| gastric transitional zones, areas where helicobacter treatment fails: results of a treatment trial using the sydney strain mouse model. | current combination therapies cure helicobacter pylori infection in 75 to 85% of cases. however, many treatment failures are not explained by antibiotic resistance. our goal was to explore treatment failures under in vivo conditions by using the h. pylori sydney strain (ss1) mouse model. mice infected with h. pylori (ss1) were treated with monotherapies or combination therapies used in human trials. bacterial levels and distribution of organisms within the stomach were assessed 24 h after treatm ... | 2003 | 12821476 |
| effect of omeprazole 10 mg on intragastric ph in three different cyp2c19 genotypes, compared with omeprazole 20 mg and lafutidine 20 mg, a new h2-receptor antagonist. | omeprazole 10 mg is used as maintenance therapy for gastro-oesophageal reflux disease, but previous reports have not mentioned the potency of its acid suppression. | 2003 | 14653835 |
| [the prospects of vaccination against h. pylori infection]. | h. pylori causes numerous gastroduodenal diseases including malignancies. although eradication of h. pylori using antibiotics is clinically performed, it is sometimes unsuccessful because of resistant bacteria and patient non-compliance with treatment. therefore prophylactic and therapeutic vaccination against h. pylori infection is necessary for clinical use but it is still under study. h. pylori is widely believed to be transmitted in childhood. if long and chronic h. pylori infection causes g ... | 2003 | 12607329 |
| [tailor-made medicine in helicobacter pylori eradication therapy]. | pharmacokinetic profiles of omeprazole and lansoprazole were well correlated with the cyp2c19 genotype. the heterozygous extensive metabolizer was slightly different from the homozygote, but there was no statistically significant difference. the cyp2c19 genotype dependence found for lansoprazole was not obvious compared with omeprazole. as for rabeprazole, the pharmacokinetic profile was independent of the cyp2c19 genotype. cyp2c19 genotyping can provide a new strategy to choose an optimal regim ... | 2003 | 12607330 |
| gastric epithelial reactive oxygen species prevent normoxic degradation of hypoxia-inducible factor-1alpha in gastric cancer cells. | the expression of hypoxia inducible factor (hif)-1alpha protein is tightly regulated by cellular oxygen status. namely, hif-1alpha protein is degraded rapidly in normoxic cells, whereas hypoxia stabilizes hif-1alpha to transactivate hypoxia-responsive genes. here we show that hif-1alpha protein is expressed aberrantly in gastric cancer cells under normoxia in a reactive oxygen species (ros)-dependent manner. the normoxic expression of hif-1alpha in concordance with its dna binding activity enhan ... | 2003 | 12538497 |
| long-term treatment with sterigmatocystin, a fungus toxin, enhances the development of intestinal metaplasia of gastric mucosa in helicobacter pylori-infected mongolian gerbils. | helicobacter pylori is a human gastric carcinogen. sterigmatocystin (st), a fungus toxin, is a risk factor of gastric cancer. cytotoxin-vacuolation toxin a (vaca) present in supernatants of h. pylori suspensions can cause gastritis and ulcer. the aim of this study was to examine the effects of h. pylori, st and vaca in mongolian gerbils. | 2003 | 12739707 |
| molecular mimicry in atherosclerosis: a role for heat shock proteins in immunisation. | atherosclerosis has long been recognised as having an inflammatory component, and this has a particularly important bearing on to its clinical complications as it may result in plaque instability. results of recent epidemiological studies have reinforced the potential importance of this aspect of the disease. positive associations have been reported between exposure to several specific pathogens, and future risk of coronary heart disease (chd). whilst it is possible that each individual organism ... | 2003 | 12818399 |
| catalase (kata) and kata-associated protein (kapa) are essential to persistent colonization in the helicobacter pylori ss1 mouse model. | helicobacter pylori infects the human gastric mucosa and elicits an aggressive inflammatory response. despite the severity of the inflammatory response, the bacterium is able to persist and cause a chronic infection. it is believed that antioxidant defence mechanisms enable this organism to persist. wild-type h. pylori strain ss1, and kata- and kapa-deficient mutants, were used to infect c57/bl6 mice to test this hypothesis. neither kata nor kapa was essential for the initial colonization of h. ... | 2003 | 12634335 |
| cloning, expression and purification of three chaperonin 60 homologues. | the chaperonin 60 (cpn60) proteins have, in addition to their well-known functions of protein folding and protection, a range of intercellular signalling activities. as part of a study to investigate the biological activity of the cpn60 proteins, particularly from pathogenic organisms, we have cloned and expressed three cpn60 proteins from homo sapiens, helicobacter pylori and chlamydia pneumoniae. the cpn60 proteins were purified to apparent homogeneity using a combination of nickel column affi ... | 2003 | 12651007 |
| mutant p53 expression and apoptotic activity of helicobacter pylori positive and negative gastritis in correlation with the presence of intestinal metaplasia. | mutation of the p53 gene is detectable in most cases of gastric cancer, as it is the most common genetic alteration in human malignancies. it is also well documented that helicobacter pylori infection plays an important role in gastric carcinogenesis. there is still no clarification, however, concerning how genetic instability influences the homeostasis of gastric epithelium. we have studied the effect of h. pylori infection on apoptosis of the antral epithelium in the presence/absence of intest ... | 2003 | 12655259 |
| molecular cloning of p53 cdna of mongolian gerbil and establishment of yeast p53 functional assay system. | epidemiological studies have shown a correlation between helicobacter pylori infection and human gastric carcinogenesis. a mongolian gerbil model has demonstrated that h. pylori infection induced gastric carcinoma. however, the disadvantage of this animal model is a lack of information regarding the cellular genes involved in oncogenesis. mutation of the p53 gene is one of the most common steps in gastric carcinogenesis. in this study, we aimed to clone the p53 gene of the mongolian gerbil and d ... | 2003 | 12662374 |
| topology and membrane interaction of helicobacter pylori comb proteins involved in natural transformation competence. | the human gastric pathogen helicobacter pylori is naturally competent for genetic transformation. the h. pylori comb gene duster encodes the virb4-homologous atpase comb4 and the structural proteins comb7-comb10, which share significant sequence identity to the agrobacterium tumefaciens virb-encoded type iv secretion system. to study the topology of the comb7-10 proteins, we applied tnmax transposon mutagenesis by generating fusions of comb proteins with mature beta-lactamase (blam) or alkaline ... | 2003 | 12868652 |
| lafutidine, a novel histamine h2-receptor antagonist, vs lansoprazole in combination with amoxicillin and clarithromycin for eradication of helicobacter pylori. | in contrast to the growing amount of data concerning proton pump inhibitor-based triple therapy for helicobacter pylori infection, it is still controversial whether proton pump inhibitor can be replaced by h2 receptor antagonist without compromising efficacy. lafutidine is a novel potent h2 receptor antagonist with gastroprotective activities such as enhancement of gastric mucosal blood flow. | 2003 | 12662378 |
| the neutrophil-activating protein of helicobacter pylori (hp-nap) activates the mapk pathway in human neutrophils. | infection by helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa characterized by the infiltration of neutrophils andmononuclear inflammatory cells. the neutrophil-activating protein of helicobacter pylori (hp-nap) is a virulence factor that activates neutrophils, monocytes, and mast cells. however, the mechanism by which hp-nap activates these cells is not fully understood. here, we show that hp-nap induces extracellular regulate ... | 2003 | 12672049 |
| influence of gastrin on the expression of cyclooxygenase-2, hepatocyte growth factor and apoptosis-related proteins in gastric epithelial cells. | several studies have shown a link between gastrin and gastric cancer, both in humans and animals, especially infected with helicobacter pylori (h. pylori). however, the exact role of hypergastrinemia in gastric carcinogenesis remains still undetermined. the aim of the present study was to evaluate the interaction between gastrin, cyclooxygenase-2 (cox-2), hepatocyte growth factor (hgf) and apoptosis-related proteins (bax, bcl-2, caspase-3, survivin) in cultured gastric epithelial cancer cells. | 2003 | 12674216 |
| variable frequencies of t(11;18)(q21;q21) in malt lymphomas of different sites: significant association with caga strains of h pylori in gastric malt lymphoma. | t(11;18)(q21;q21) is a specific chromosomal translocation associated with mucosa-associated lymphoid tissue (malt) lymphoma. it fuses the amino terminal of the api2 gene to the carboxyl terminal of the malt1 gene and generates a chimeric fusion product. although the translocation is frequently detected in gastric and pulmonary malt lymphoma, its incidence in malt lymphomas from other sites is largely unknown. it also remains unknown whether the occurrence of the translocation is influenced by th ... | 2003 | 12676782 |
| expression cloning of a periodontitis-associated apoptotic effector, cage homologue, in actinobacillus actinomycetemcomitans. | to study anti-bacterial immunity and to identify critical bacterial antigens associated with specific periodontal infection, we screened the genomic library of actinobacillus actinomycetemcomitans, a major gram(-) anaerobe causing human periodontitis, by expression cloning using disease-associated periodontal cd4(+)t cells derived from hupbl-engrafted nod/scid mice. here, we report one of the novel genes identified and designated, cage homologue (in short: cage) of a. actinomycetemcomitans, whic ... | 2003 | 12684047 |
| decreased epithelial cytokine responses in the duodenal mucosa of helicobacter pylori-infected duodenal ulcer patients. | helicobacter pylori colonizes the human stomach and areas of gastric metaplasia in the duodenum, but only a minority of those that are infected develop symptoms, e.g., peptic ulcers. although most ulcers occur in the duodenum, almost all studies of mucosal immune responses against the infection have been limited to responses in the stomach. in the present study we evaluated whether there are differences in the levels of proinflammatory cytokines as well as immunoregulatory cytokines in the duode ... | 2003 | 12522049 |
| association of cyclooxygenase-2 expression with hp-caga infection in gastric cancer. | to observe the expression of cyclooxygenase-2 (cox-2) and to investigate the association between cox-2 expression and infection with cytotoxic-associated gene a (caga) positive strain helicobacter pylori (hp) in human gastric cancer, and subsequently to provide fresh ideas for the early prevention of gastric cancer. | 2003 | 12532440 |
| impact of clarithromycin resistance and cyp2c19 genetic polymorphism on treatment efficacy of helicobacter pylori infection with lansoprazole- or rabeprazole-based triple therapy in japan. | helicobacter pylori treatment failure is thought to be due mainly to polymorphic cytochrome p450 2c19 (cpy2c19) genetic polymorphism, associated with proton pump inhibitor metabolism, and antimicrobial susceptibility. this report has ascertained which was more important, cpy2c19 polymorphism or antimicrobial susceptibility, when using 1-week lansoprazole-based or rabeprazole-based triple therapy in japan. | 2003 | 12544691 |
| the dendritic cell-specific c-type lectin dc-sign is a receptor for schistosoma mansoni egg antigens and recognizes the glycan antigen lewis x. | schistosoma mansoni soluble egg antigens (seas) are crucially involved in modulating the host immune response to infection by s. mansoni. we report that human dendritic cells bind seas through the c-type lectin dendritic cell-specific icam-3-grabbing nonintegrin (dc-sign). monoclonal antibodies against the carbohydrate antigens lewisx (lex) and galnacbeta1-4(fucalpha1-3)glcnac (ldnf) inhibit binding of dc-sign to seas, suggesting that these glycan antigens may be critically involved in binding. ... | 2003 | 12626400 |
| endotoxic and immunobiological activities of a chemically synthesized lipid a of helicobacter pylori strain 206-1. | a synthetic lipid a of helicobacter pylori strain 206-1 (compound hp206-1), which is similar to its natural lipid a, exhibited no or very low endotoxic activities as compared to escherichia coli-type synthetic lipid a (compound 506). furthermore, compound hp206-1 as well as its natural lipid a demonstrated no or very low mitogenic responses in murine spleen cell. on the other hand, compound hp206-1 showed a weaker but significant production of interleukin-8 in a gastric cancer cell line, mkn-1, ... | 2003 | 12727359 |
| the rapid detection of low molecular mass proteins differentially expressed under biological stress for four helicobacter spp. using proteinchip technology. | helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. in addition h. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from h. ... | 2003 | 12627380 |
| t helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions. | atherosclerotic lesions are infiltrated by macrophages and t lymphocytes, potentially reactive to pathogens. we studied in vivo activated t lymphocytes that infiltrate atherosclerotic plaques of helicobacter pylori-infected patients with or without anti-chlamydia pneumoniae antibodies. in all atherosclerotic lesions, t helper type 1 (th1) cells were predominant. c. pneumoniae-specific t cells were detected only in the plaques of anti-c. pneumoniae seropositive patients, whereas h. pylori-specifi ... | 2003 | 12740434 |
| the impact of parietal cells on helicobacter pylori tropism and host pathology: an analysis using gnotobiotic normal and transgenic mice. | helicobacter pylori infection of the human stomach is common and typically benign, although a subset of hosts develops severe pathology. infection occurs in an organ with distinct microenvironments characterized by pronounced differences in the composition of acid-producing parietal cells. in this study, we examine determinants of bacterial tropism to various gastric niches by using germ-free normal and transgenic mice with an engineered parietal cell ablation. mice were colonized for 8 weeks wi ... | 2003 | 12629225 |
| helicobacter pylori induces apoptosis in barrett's-derived esophageal adenocarcinoma cells. | helicobacter pylori may protect against the development of dysplasia in barrett's epithelium of patients with gastroesophageal reflux disease. the aim of this study was to determine whether h. pylori preferentially induces apoptosis in barrett's-derived cancer cells compared to normal cells. a barrett's-derived adenocarcinoma cell line (oe33) was grown. h. pylori wild-type, isogenic vaca-, caga(-), and picb-/cage- mutant strains were grown on agar plates. intact or sonicated bacteria were used t ... | 2003 | 12559187 |
| multiple infections and subsequent cardiovascular events in the heart outcomes prevention evaluation (hope) study. | limited prospective epidemiological data are available on the relation between exposure to chlamydia pneumoniae, helicobacter pylori, cytomegalovirus (cmv), and hepatitis a virus (hav), individually or as a total pathogen score, and human cardiovascular (cv) disease. | 2003 | 12538424 |
| plural transformation-processes from spiral to coccoid helicobacter pylori and its viability. | helicobacter pylori (h. pylori), present in a half of the world's population, is a very successful pathogen. the infection by this bacterium causes several gastric diseases in human. h. pylori is morphologically divided into two types; a spiral and a coccoid form. both types are observed in human stomach. although the former is converted into the latter in vitro, the process of coccoid formation remains obscure. furthermore, whether coccoid forms possess viability arouses much controversy among ... | 2003 | 12504609 |
| is intestinal metaplasia of the stomach reversible? | intestinal metaplasia (im) of the stomach is a risk factor in developing intestinal-type gastric cancer and hence the question of reversibility is vital. there is emerging epidemiological evidence that with long term follow up, im may be reversible although a combination of antioxidant agents and eradication of h pylori may be necessary to achieve this. the pathogenesis of im is currently being elucidated and it is likely that a combination of bacterial, host, and environmental factors will be s ... | 2003 | 12477745 |
| increased zinc finger protein zfoc1 transcripts in gastric cancer compared with normal gastric tissue. | screening of cdna arrays of the image library identified human zfoc1 as a differentially expressed cdna that was upregulated in kato iii gastric cancer cells following stimulation with the gastric pathogen helicobacter pylori. | 2003 | 12782764 |
| review article: nsaids, gastroprotection and cyclo-oxygenase-ii-selective inhibitors. | in patients at high risk of nsaid-associated serious upper gastrointestinal complications, gastroprotection with misoprostol or a proton pump inhibitor should be considered. only misoprostol, 800 micro g/day, has been shown to reduce serious upper gastrointestinal complications in a large clinical outcome trial. the benefit of helicobacter pylori eradication in reducing nsaid-associated gastrointestinal toxicity is controversial, and routine testing for and eradication of h. pylori in nsaid user ... | 2003 | 12562444 |
| chemokine receptor 5 expression in gastric mucosa of helicobacter pylori-infected and noninfected children. | experimental data from human adults or animal models indicate that the helicobacter pylori-specific immune response is dominated by inflammatory t cells of the th1 type. to investigate whether a th1 immune response is established in early h. pylori infection, gastric biopsy samples from 70 children were subjected to immunohistochemical analysis. to this end, t cells, b cells, monocytes, neutrophils, and chemokine receptor 5 (ccr5)-expressing (ccr5(+)) cells, which are associated with th1 immune ... | 2003 | 12522035 |