Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| evidence for a "wattle and daub" model of the cyst wall of entamoeba. | the cyst wall of entamoeba invadens (ei), a model for the human pathogen entamoeba histolytica, is composed of fibrils of chitin and three chitin-binding lectins called jacob, jessie3, and chitinase. here we show chitin, which was detected with wheat germ agglutinin, is made in secretory vesicles prior to its deposition on the surface of encysting ei. jacob lectins, which have tandemly arrayed chitin-binding domains (cbds), and chitinase, which has an n-terminal cbd, were each made early during ... | 2009 | 19578434 |
| a possible cluster of sexually transmitted entamoeba histolytica: genetic analysis of a highly virulent strain. | transmission of entamoeba histolytica generally occurs by fecal excretion of cysts followed by oral ingestion of contaminated food or water. however, fecal-oral transmission may occur within households and long-term care institutions, and sexual transmission occurs among men who have sex with men. epidemiologically linked clusters of e. histolytica infection are rare in industrialized countries. we report such a sexually linked cluster in canada. | 2009 | 19580413 |
| crude extracts of, and purified compounds from, pterocarpus angolensis, and the essential oil of lippia javanica: their in-vitro cytotoxicities and activities against selected bacteria and entamoeba histolytica. | in a recent study, various extracts of pterocarpus angolensis were prepared and tested against bacteria. the acetone extract was found to be the most active against all the bacteria investigated, with minimum inhibitory concentrations varying from 0.0156 mg/ml against staphylococcus aureus to 2 mg/ml against enterobacter cloacae. seven pure compounds were subsequently isolated from the ethanol extract of p. angolensis. using several chromatographic and spectroscopic methods, the structures of fi ... | 2009 | 19583913 |
| aids-associated parasitic diarrhoea. | since the advent of human immunodeficiency virus infection, with its profound and progressive effect on the cellular immune system, a group of human opportunistic pathogens has come into prominence. opportunistic parasitic infection can cause severe morbidity and mortality. because many of these infections are treatable, an early and accurate diagnosis is important. this can be accomplished by a variety of methods such as direct demonstration of parasites and by serological tests to detect antig ... | 2009 | 19584497 |
| rapid diagnosis of intestinal parasitic protozoa, with a focus on entamoeba histolytica. | entamoeba histolytica is an invasive intestinal pathogenic parasitic protozoan that causes amebiasis. it must be distinguished from entamoeba dispar and e. moshkovskii, nonpathogenic commensal parasites of the human gut lumen that are morphologically identical to e. histolytica. detection of specific e. histolytica antigens in stools is a fast, sensitive technique that should be considered as the method of choice. stool real-time pcr is a highly sensitive and specific technique but its high cost ... | 2009 | 19584941 |
| synthesis, characterization, antiamoebic activity and toxicity of novel bisdioxazole derivatives. | cyclization of benzene-1,4-dicarbaldehyde dioxime 1 with different aromatic aldehydes in inert atmosphere yielded the corresponding new bisdioxazoles 2-11. the structure of 2-11 was elucidated by spectral data. in vitro antiamoebic activity was performed against hm1:imss strain of entamoeba histolytica. the results showed that the compounds 3 (ic(50)=1.22 microm), 4 (ic(50)=1.41 microm), 7 (ic(50)=1.05 microm) and 10 (ic(50)=1.01 microm) exhibited better antiamoebic activity than the standard dr ... | 2009 | 19589625 |
| dna repair mechanisms in eukaryotes: special focus in entamoeba histolytica and related protozoan parasites. | eukaryotic cell viability highly relies on genome stability and dna integrity maintenance. the cellular response to dna damage mainly consists of six biological conserved pathways known as homologous recombination repair (hrr), non-homologous end-joining (nhej), base excision repair (ber), mismatch repair (mmr), nucleotide excision repair (ner), and methyltransferase repair that operate in a concerted way to minimize genetic information loss due to a dna lesion. particularly, protozoan parasites ... | 2009 | 19591963 |
| [antigen detection methods in diagnosis of amebiasis, giardiasis and cryptosporidiosis]. | amebiasis is a significant health problem in developing countries. humans are infected by two morphologically identical species of entamoeba. entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. giardia intestinalis infection is seen worldwide and in all age groups. but giardiasis is especially prevalent in countries with poor sanitation and unsafe water, where it's responsible for most cases of childhood diarrhea. cryptosporidium parvum, a protozoo ... | 2009 | 19598091 |
| genomic dna k-mer spectra: models and modalities. | the empirical frequencies of dna k-mers in whole genome sequences provide an interesting perspective on genomic complexity, and the availability of large segments of genomic sequence from many organisms means that analysis of k-mers with non-trivial lengths is now possible. | 2009 | 19814784 |
| intestinal parasitic infection and associated risk factors, among children presenting at outpatient clinics in manaus, amazonas state, brazil. | in a cross-sectional study, the prevalences of intestinal parasitic infection and the associated risk factors were explored among children aged 0-10 years attending outpatient clinics in manaus, the capital city of the north brazilian state of amazonas. data indicating socio-economic level and demographic factors were collected in interviews with the accompanying parent or guardian of each child. parasitic infections were detected by the microscopical examination of faecal samples, while nutriti ... | 2009 | 19825280 |
| deficient serum mannose-binding lectin levels and mbl2 polymorphisms increase the risk of single and recurrent cryptosporidium infections in young children. | mannose-binding lectin (mbl) is an evolutionarily conserved protein that functions in human innate immunity by binding to microbial surfaces and promoting opsonophagocytosis. mbl has been shown to bind to cryptosporidium sporozoites, and earlier work has suggested that the protective role of mbl may be most important in childhood. we evaluated the association between polymorphisms in the mbl gene (mbl2), serum mbl deficiency, and infection with cryptosporidium, entamoeba histolytica, and giardia ... | 2009 | 19827946 |
| identification of entamoeba histolytica and entamoeba dispar by pcr assay of fecal specimens obtained from thai/myanmar border region. | due to the indistinguishable morphology between entamoeba histolytica (pathogenic) and entamoeba dispar (non pathogenic), pcr-based assays were conducted. based on microscopy, suspected entamoeba cells were detected in 30 out of 455 fecal samples obtained from individuals residing at thai/myanmar border region. the target genes for pcr amplification included genes encoding small subunit rrna (ssu-rrna), chitinase and serine rich entamoeba protein. pcr primers derived from ssu-rrna gene amplified ... | 2009 | 19842426 |
| serum leptin concentrations in patients with intestinal parasites. | leptin is a protein hormone controlling food intake and energy expenditure. in all infections including parasitic infections there is loss of appetite and anorexia. the aim of the present study was to clarify the relationship between intestinal parasites and serum leptin concentrations in children and adults. forty patients with intestinal parasites and 34 healthy subjects took part in this study. body weight, height and body mass index (bmi) were measured for all patients and controls. patients ... | 2009 | 19851965 |
| [epidemiological investigation on an outbreak of amoebic dysentery in jiangshan city of zhejiang province]. | the investigation was carried out from 15 may to 10 june 2006 among diarrhea patients of two schools and periphery residents in hecun town, jiangshan city. stool samples were examined for entamoeba histolytica. water samples were taken for microbial analysis. 31 cases with e. histolytica were found,with 74.2% (23 cases) of students and preschool children. 9 cases were found in liuyi kindergarten and 8 cases in hecun central primary school with a prevalence of 7.4% and 0.65%, respectively. among ... | 2009 | 19856515 |
| methionine gamma-lyase: the unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers. | sulfur-containing amino acids (saas) are essential components in many biological processes and ubiquitously distributed to all organisms. both biosynthetic and catabolic pathways of saas are heterogeneous among organisms and between developmental stages, and regulated by the environmental changes. limited lineage of organisms ranging from archaea to plants, but not human, possess a unique enzyme methionine gamma-lyase (mgl, ec 4.4.1.11) to directly degrade saa to alpha-keto acids, ammonia, and v ... | 2009 | 19859976 |
| prevalence of multi-gastrointestinal infections with helminth, protozoan and campylobacter spp. in guatemalan children. | the prevalence of multi-infections with helminthes, protozoans and campylobacter spp. in guatemalan children is a reflection of differences in the risk factors related to pathogen transmission. | 2009 | 19759480 |
| illness in long-term travelers visiting geosentinel clinics. | length of travel appears to be associated with health risks. geosentinel surveillance network data for 4,039 long-term travelers (trip duration >6 months) seen after travel during june 1, 1996, through december 31, 2008, were compared with data for 24,807 short-term travelers (trip duration <1 month). long-term travelers traveled more often than short-term travelers for volunteer activities (39.7% vs. 7.0%) and business (25.2% vs. 13.8%). more long-term travelers were men (57.2% vs. 50.1%) and e ... | 2009 | 19891865 |
| mitosomes in entamoeba histolytica contain a sulfate activation pathway. | hydrogenosomes and mitosomes are mitochondrion-related organelles in anaerobic/microaerophilic eukaryotes with highly reduced and divergent functions. the full diversity of their content and function, however, has not been fully determined. to understand the central role of mitosomes in entamoeba histolytica, a parasitic protozoon that causes amoebic dysentery and liver abscesses, we examined the proteomic profile of purified mitosomes. using 2 discontinuous percoll gradient centrifugation and m ... | 2009 | 19995967 |
| fulminant colitis and toxic myocarditis: a unifying cause? | 2009 | 20006131 | |
| use of bacterially expressed dsrna to downregulate entamoeba histolytica gene expression. | modern rna interference (rnai) methodologies using small interfering rna (sirna) oligonucleotide duplexes or episomally synthesized hairpin rna are valuable tools for the analysis of gene function in the protozoan parasite entamoeba histolytica. however, these approaches still require time-consuming procedures including transfection and drug selection, or costly synthetic molecules. | 2009 | 20037645 |
| association of malnutrition with amebiasis. | observation of a cohort of preschool children in dhaka, bangladesh, is beginning to reveal the contributions of environment, host, and parasite to amebiasis. reviewed here are the associations and interactions of malnutrition, iga and interferon-gamma, human leukocyte antigen alleles, and parasite genotypes to the outcome of infection. future efforts aimed at understanding the mechanisms of these effects are described. | 2009 | 19906225 |
| characterization of the restriction enzyme-like endonuclease encoded by the entamoeba histolytica non-long terminal repeat retrotransposon ehline1. | the genome of the human pathogen entamoeba histolytica, a primitive protist, contains non-long terminal repeat retrotransposable elements called ehlines. these encode reverse transcriptase and endonuclease required for retrotransposition. the endonuclease shows sequence similarity with bacterial restriction endonucleases. here we report the salient enzymatic features of one such endonuclease. the kinetics of an ehline1-encoded endonuclease catalyzed reaction, determined under steady-state and si ... | 2009 | 19878305 |
| [pleuro-pulmonary manifestations disclosing hepatic amoebiasis]. | during a mission in ex-yugoslavia between 2001 and 2004, three french soldiers were sent home because of right pneumopathy, right pleurisy after appendicectomy, haemoptysis and liver haematoma, respectively. they previously were stationed in africa and/or south america. the initial diagnosis was quickly modified: pleuropulmonary manifestations of amoebic hepatic abscess in two cases, and pleuropulmonary amoebiasis in the last case. the outcome was favourable with standard anti-amoebic treatment. ... | 2009 | 19878805 |
| an ex-vivo human intestinal model to study entamoeba histolytica pathogenesis. | amoebiasis (a human intestinal infection affecting 50 million people every year) is caused by the protozoan parasite entamoeba histolytica. to study the molecular mechanisms underlying human colon invasion by e. histolytica, we have set up an ex vivo human colon model to study the early steps in amoebiasis. using scanning electron microscopy and histological analyses, we have established that e. histolytica caused the removal of the protective mucus coat during the first two hours of incubation, ... | 2009 | 19936071 |
| broad early immune response of porcine epithelial jejunal ipi-2i cells to entamoeba histolytica. | amoebiasis caused by entamoebahistolytica triggers an acute inflammatory response at early stages of intestinal infection. the patho-physiological study of intestinal amoebiasis requires the development of powerful animal models. swine provide robust model for human diseases and they could be used to study intestinal amoebiasis. here, we introduce an in vitro model of swine intestinal epithelial cell (ipi-2i) co-cultured with e. histolytica. intestinal epithelial cells (iecs) have crucial roles ... | 2009 | 19110312 |
| update on human infections caused by intestinal protozoa. | to update the reader on the recent literature discussing human infections due to intestinal protozoa. | 2009 | 19119509 |
| entamoeba histolytica: oxygen resistance and virulence. | entamoeba histolytica virulence has been attributed to several amoebic molecules such as adhesins, amoebapores and cysteine proteinases, but supporting evidence is either partial or indirect. in this work we compared several in vitro and in vivo features of both virulent e. histolytica (veh) and non-virulent e. histolytica (nveh) axenic hm-1 imss strains, such as complement resistance, proteinase activity, haemolytic, phagocytic and cytotoxic capacities, survival in mice caecum, and susceptibili ... | 2009 | 19073188 |
| characterization of entamoeba histolytica intermediate subunit lectin-specific human monoclonal antibodies generated in transgenic mice expressing human immunoglobulin loci. | four fully human monoclonal antibodies (mabs) to entamoeba histolytica intermediate subunit lectin (igl) were prepared in xenomouse mice, which are transgenic mice expressing human immunoglobulin loci. examination of the reactivities of these mabs to recombinant igl1 and igl2 of e. histolytica showed that xehi-20 {immunoglobulin g2(kappa) [igg2(kappa)]} and xehi-28 [igg2(kappa)] were specific to igl1, xehi-b5 [igg2(kappa)] was specific to igl2, and xehi-h2 [igm(kappa)] was reactive with both igl ... | 2009 | 19001071 |
| entamoeba histolytica: effect on virulence, growth and gene expression in response to monoxenic culture with escherichia coli 055. | monoxenic cultivation of pathogenic entamoeba histolytica trophozoites with escherichia coli serotype 055 which binds strongly to the gal/galnac amoebic lectin, markedly improved the growth of e. histolytica and produced a significant decrease in cysteine proteinase activity and a lower cytopathic activity on monolayer cells after 3 months of monoxenic culture. however, after long term monoxenic culture (12 months) the proteolytic and cytopathic activities were recovered and the amoebic growth r ... | 2009 | 19014938 |
| giardia, entamoeba, and trichomonas enzymes activate metronidazole (nitroreductases) and inactivate metronidazole (nitroimidazole reductases). | infections with giardia lamblia, entamoeba histolytica, and trichomonas vaginalis, which cause diarrhea, dysentery, and vaginitis, respectively, are each treated with metronidazole. here we show that giardia, entamoeba, and trichomonas have oxygen-insensitive nitroreductase (ntr) genes which are homologous to those genes that have nonsense mutations in metronidazole-resistant helicobacter pylori isolates. entamoeba and trichomonas also have nim genes which are homologous to those genes expressed ... | 2009 | 19015349 |
| trophozoites of entamoeba histolytica express a cd59-like molecule in human colon. | in vitro studies have proved the presence of epitopes of cd59 in the surface of trophozoites of entamoeba histolytica (e. histolytica). however, it has not been proved if cd59 molecules are expressed in the surface during the trophozoites' tissue invasion. the aim of the present study was to determine whether the complement-regulatory protein cd59 is present on trophozoites of e. histolytica in human colon. eleven specimens of amoebic colitis were studied by immunohistochemistry and electron mic ... | 2009 | 19018568 |
| high frequency of parasitic and viral stool pathogens in patients with active ulcerative colitis: report from a tropical country. | diarrhoeal relapses in patients with ulcerative colitis (uc) may be associated with enteric infections and its diagnosis may lessen avoidable exposure to corticosteroids and/or immunosuppressants. the purpose of this study was to assess the frequency of stool pathogens (parasitic and viral) in patients with active uc. | 2009 | 19040190 |
| cloning and identification of a gene coding for a 26-kda hemoglobin-binding protein from entamoeba histolytica. | the capability of entamoeba histolytica to use hemoglobin (hb) as an iron source has been documented. however, the underlying mechanism to acquire iron from this source is poorly understood. in the present work, an in silico analysis in the e. histolytica genome (pathema database) allowed us to identify a gene coding for a putative 26-kda protein (ehhmbp26) which contains the motifs necessary for hb-binding. the purified ehhmbp26 protein was able to bind hb. albeit with less efficiency, trophozo ... | 2009 | 19041684 |
| promoter analysis of palindromic transcription units in the ribosomal dna circle of entamoeba histolytica. | rrna genes of entamoeba histolytica are organized as palindromic ribosomal dna (rdna) units (i and ii) in a 24.5-kb circle. although the two rdnas are identical in sequence, their upstream spacers are completely different. since the intergenic sequences (igs) of all rdna copies in other organisms are conserved and contain transcription regulatory sequences, the lack of sequence conservation in the igs prompted the question of whether both rdnas are indeed transcriptionally active. we mapped the ... | 2009 | 18978203 |
| effect of tourism and trade on intestinal parasitic infections in guatemala. | a survey was performed to determine if infection with gastrointestinal parasites differs between the rural and urban poor inhabitants of guatemala. a total of 317 stool samples from children in two towns, one rural and one urban, were examined using the formalin-ether concentration method. the overall prevalence of parasites in infected children was 67%, 20%, 30%, and 22%, respectively for ascaris lumbricoides, trichuris trichiura, giardia duodenalis and entamoeba histolytica in the rural town o ... | 2009 | 18941875 |
| recent insights into entamoeba development: identification of transcriptional networks associated with stage conversion. | entamoeba histolytica is an important human pathogen and a leading parasitic cause of death globally. the parasite life cycle alternates between the trophozoite form, which is motile and causes invasive disease and the cyst stage, which is environmentally resistant and transmits infection. understanding the triggers that initiate stage conversion is an important yet understudied area of investigation. recent progress in dissecting the transcriptional networks that regulate e. histolytica develop ... | 2009 | 18938171 |
| public health importance of non-biting cyclorrhaphan flies. | this study was carried out to determine the role of non-biting cyclorrhaphan flies as carriers of human intestinal parasites at woreta, northwestern ethiopia. in total, 6530 flies were collected from four breeding sites and then examined for human intestinal parasites, mainly using the formol-ether concentration method. fly species identified were musca domestica (32.9%), chrysomya rufifacies (32.6%), musca sorbens (23%), lucina cuprina (4.7%), calliphora vicina (2.8%), chrysomya bezziana (2.3%) ... | 2009 | 18817940 |
| entamoeba histolytica uses ferritin as an iron source and internalises this protein by means of clathrin-coated vesicles. | entamoeba histolytica is a parasitic protozoan that produces dysentery and often reaches the liver, leading to abscess formation. ferritin is an iron-storage protein that is mainly found in liver and spleen in mammals. the liver contains a plentiful source of iron for amoebae multiplying in that organ, making it a prime target for infection since iron is essential for the growth of this parasite. the aim of this study was to determine whether trophozoites are able to take up ferritin and interna ... | 2009 | 18848948 |
| isoform-dependent feedback regulation of serine o-acetyltransferase isoenzymes involved in l-cysteine biosynthesis of entamoeba histolytica. | serine acetyltransferase (sat; ec 2.3.1.30) catalyzes the coa-dependent acetylation of the side chain hydroxyl group of l-serine to form o-acetyl serine, in the first step of the l-cysteine biosynthetic pathway. since this pathway is selectively present in a few parasitic protists and absent in mammals, it represents a reasonable target to develop new chemotherapeutics. entamoeba histolytica apparently possesses three sat isotypes (ehsat1-3) showing 48-73% mutual identity, a calculated molecular ... | 2009 | 18851994 |
| blastocystis exhibits inter- and intra-subtype variation in cysteine protease activity. | blastocystis is an enteric protistan parasite of zoonotic potential and poorly understood pathogenesis. we have previously reported that blastocystis cysteine proteases can degrade human secretory iga and are also responsible for the induction of il-8 response in colonic epithelial cells in vitro. differences in virulence between blastocystis subtypes have been reported recently in both animal models and clinical studies, although cellular mechanisms for these differences are currently unknown. ... | 2009 | 18846388 |
| polymorphonuclear neutrophil infiltration intensity as consequence of entamoeba histolytica density in amebic colitis. | it has been suggested that the damage observed in invasive amebiasis is related to interactions between polymorphonuclear leukocytes (pmn) and entamoeba histolytica trophozoites. we analyzed the relation between infiltrating inflammatory cell populations and e. histolytica density in intestinal amebic lesions. | 2009 | 18831680 |
| development of the gateway system for cloning and expressing genes in entamoeba histolytica. | the early branching eukaryote entamoeba histolytica is a human parasite that is the etiologic agent of amebic dysentery and liver abscess. the sequencing of the e. histolytica genome combined with the development of an e. histolytica microarray has resulted in the identification of several distinct gene expression profiles associated with virulence. the function of many modulated transcripts is unknown and their role in pathogenicity is unclear. they however represent a pool of potential virulen ... | 2009 | 18822389 |
| purification, refolding and autoactivation of the recombinant cysteine proteinase ehcp112 from entamoeba histolytica. | the cysteine proteinase ehcp112 and the adhesin ehadh112 assemble to form the ehcpadh complex involved in entamoeba histolytica virulence. to further characterize this cysteine proteinase, the recombinant full-length ehcp112 enzyme was expressed and purified under denaturing conditions. after a refolding step under reductive conditions, the inactive precursor (ppehcp112) was processed to a 35.5 kda mature and active enzyme (ehcp112). the thiol specific inhibitor e-64, but not serine or aspartic ... | 2009 | 18824105 |
| entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis. | upon host infection, the protozoan parasite entamoeba histolytica is confronted with reactive oxygen and nitrogen species and must survive these stresses in order to cause invasive disease. we analysed the parasite's response to oxidative and nitrosative stresses, probing the transcriptional changes of trophozoites of a pathogenic strain after a 60 min exposure to h2o2 (1 mm) or a no donor (dipropylenetriamine-nonoate, 200 microm), using whole-genome dna microarrays. genes encoding reactive oxyg ... | 2009 | 18778413 |
| antiamoebic coumarins from the root bark of adina cordifolia and their new thiosemicarbazone derivatives. | in continuation of our search for potential antiamoebic agents from folklore indian medicinal plants, we found that the benzene and ethyl acetate extracts from the root bark of adina cordifolia exhibited strong antiamoebic activity with ic(50) values of 2.92 and 2.50 microg/ml, respectively. bioassay-guided fractionation of benzene and ethyl acetate extracts led to the isolation of 7-hydroxycoumarin (umbelliferone 1) and 7-beta-d-glucosylcoumarin (skimmin 2), respectively. umbelliferone 1 was co ... | 2009 | 18675491 |
| amebic colitis can mimic tuberculosis and inflammatory bowel disease on endoscopy and biopsy. | biopsies of 11 patients with histopathologically diagnosed amebic colitis was evaluated; endoscopically, they were suspected to have tuberculosis or inflammatory bowel disease. amebiasis was suggested in the differential diagnosis in only 3 cases. three patients had purely rectal or sigmoid involvement, whereas the others had ileocecal, cecal, ascending, or transverse colon disease. the biopsies showed cryptitis and depletion of mucin but no crypt branching. crypt abscesses were seen in one biop ... | 2009 | 18499688 |
| new derivatives of 3,5-substituted-1,4,2-dioxazoles: synthesis and activity against entamoeba histolytica. | dioxazole derivatives (1-33) were synthesized in two steps via their corresponding oximes (i-iii). all the compounds were characterized using various spectroscopic techniques. a comparative study of in vitro antiamoebic activity of these heterocyclic compounds, viz. 3-o-chloro (1-11), 3-m-chloro (12-22) and 3-p-chloro (23-33) dioxazoles having same substituents at 5-position of dioxazole ring, was performed against hm1:imss strain of entamoeba histolytica. the results showed a regular pattern in ... | 2009 | 18384916 |
| intestinal parasitosis in school going children of dharan municipality, nepal. | this community-based investigation was carried out to estimate the prevalence of intestinal parasitosis in primary school-going children of the dharan municipality, sunsari, nepal from 2007 through 2008. a total of 182 stool samples were collected from 101 male and 81 female children of ages 4 to 10 years. a questionnaire including the factors predisposing to parasitosis, was filled up by the parents of each child. parasitic examination was carried out by direct wet mount examination and concent ... | 2009 | 20306743 |
| a 23-year old male with cough, fever and blood-streaked sputum. | 2009 | 20298388 | |
| intestinal polyparasitism in a rural kenyan community. | polyparasitism seems to be a common feature in human populations in sub-saharan africa. however, very little is known about its epidemiological significance, its long term impact on human health or the types of interactions that occur between the different parasite species involved. | 2009 | 20358789 |
| prevalence of intestinal parasitic and bacterial pathogens in diarrhoeal and non-diarroeal human stools from vhembe district, south africa. | in the present study, a cross-sectional survey of intestinal parasitic and bacterial infections in relation to diarrhoea in vhembe district and the antimicrobial susceptibility profiles of isolated bacterial pathogens was conducted. stool samples were collected from 528 patients attending major public hospitals and 295 children attending two public primary schools and were analyzed by standard microbiological and parasitological techniques. entamoeba histolytica/e. dispar (34.2%) and cryptospori ... | 2009 | 20099757 |
| [intestinal parasitoses detected in primary schoolchildren in the van province]. | this study was carried out on school children in primary schools in the van province in order to investigate the distribution of intestinal parasitoses. the study was conducted in 7-15 year-old primary schoolchildren during the years from 2003-2005. stool specimens were taken from 2975 school children; of these, 1289 were female and 1686, male. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of the health research and training ... | 2009 | 20101580 |
| [intestinal parasites detected in süphan primary schoolchildren in van]. | this study was performed in order to determine the distribution of intestinal parasites among students of süphan primary school in van in 2005. in the study, stool specimens were taken from 395 students (166 girls and 229 boys) between 7-15 years of age. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of health research and training hospital, yüzüncü yil university. one or more parasite species were detected in 28.9 % of the ch ... | 2009 | 20101581 |
| [overdiagnosis of amebiasis in children with dysentery]. | there are morphologically identical amebaes, but with differences that can distinguish them; one as pathogenic: entamoeba histolytica, and the other: entamoeba dispar, as inoffensive. that brought the new hypothesis that many of the cases treated as amebiasis, weren't so. | 2009 | 20049395 |
| th-1/th-2 cytokine pattern in human amoebic colitis. | amoebiasis, caused by entamoeba histolytica, is still considered a major health problem in developing countries. since the immune response during human amoebiasis has not been clearly defined, we chose to evaluate cytokine production in patients suffering from amoebic colitis. a case-control association study was carried out on 62 subjects, including 31 patients with amoebic colitis and 31 healthy controls (age, sex and geographic region-matched). serum levels of il-12, ifn-gamma, il-13 and il-5 ... | 2009 | 20128506 |
| characteristics of amebic liver abscess in patients with or without human immunodeficiency virus. | amebic liver abscess (ala) is the most common extra-intestinal amebiasis. this study was performed to review the clinical features of patients with amebic liver abscesses and compare them in taiwanese patients with or without human immunodeficiency virus (hiv). | 2009 | 20422135 |
| undiagnosed amebic brain abscess. | we report a case of amebic brain abscess due to entamoeba histolytica. the patient was a 31-year-old man who presented with amebic liver abscess. his clinical course deteriorated in spite of proper drainage and treatment. he developed delirium, lethargy and then expired. with a history of heroin addiction, withdrawal syndrome from heroin was suspected. at autopsy, amebic abscesses were detected in the liver, large intestine, meninges and brain. a 19 cm amebic liver abscess was found in the right ... | 2009 | 20578451 |
| current and future perspectives on the chemotherapy of the parasitic protozoa trichomonas vaginalis and entamoeba histolytica. | trichomonas vaginalis and entamoeba histolytica are clinically important protozoa that affect humans. t. vaginalis produces sexually transmitted infections and e. histolytica is the causative agent of amebic dysentery. metronidazole, a compound first used to treat t. vaginalis in 1959, is still the main drug used worldwide to treat these pathogens. it is essential to find new biochemical differences in these organisms that could be exploited to develop new antiprotozoal chemotherapeutics. recent ... | 2009 | 21426030 |
| synthesis, spectral studies and antiamoebic activity of new 1-n-substituted thiocarbamoyl-3-phenyl-2-pyrazolines. | thirty new pyrazoline derivatives were synthesized by cyclization of mannich bases with thiosemicarbazides being substituted by different cyclic and aromatic amines. the structures of the compounds were elucidated by elemental analyses, uv, ir, (1)h and (13)c nmr and esi-ms spectral data. the in vitro antiamoebic activity was evaluated against entamoeba histolytica in comparison with metronidazole used as reference substance. out of the 30 compounds screened for antiamoebic activity, 10 (5, 6, 1 ... | 2009 | 18068873 |
| bis-pyrazolines: synthesis, characterization and antiamoebic activity as inhibitors of growth of entamoeba histolytica. | the cyclization of chalcone with n-4 substituted thiosemicarbazides under basic condition led to the formation of new compounds, thiocarbamoyl bis-pyrazoline derivatives. the structure of the compounds were elucidated by uv, ir, (1)h nmr, (13)c nmr and esi-ms spectral data and thermogravimetric analysis, and their purities were confirmed by elemental analyses. the antiamoebic activity of these complexes was evaluated by microdilution method against hm1:imss strain of entamoeba histolytica and th ... | 2009 | 18187238 |
| prevalence of intestinal parasitic infections among hiv patients in benin city, nigeria. | this study was carried out to determine the presence of intestinal parasites and their correlation with cd4(+) t-cell counts and demographics among human immunodeficiency virus (hiv)-positive patients in benin city, nigeria. stool specimens from 2,000 hiv-positive patients and 500 controls (hiv-negative individuals) were examined for ova, cysts, or parasites, using standard procedures. in addition, patient's blood samples were analyzed for cd4 counts by flow cytometry. an overall prevalence rate ... | 2010 | 21483561 |
| [failure to offer parasitology screening to vulnerable migrants in france: epidemiology and consequences.] | the "consultations de diagnostic et d'orientation" (cdo), created in 1998 by the paris health department, are medical consultations for vulnerable populations, which are held in municipal clinics, free of charge. more than two-thirds of cdo patients come from africa. our study was designed to assess and analyze whether these clinics offered consulting migrants screening for intestinal parasitic and schistosoma haematobium infections.material and methodsthis retrospective study included all immig ... | 2010 | 21320812 |
| infectious diarrhea: cellular and molecular mechanisms. | diarrhea caused by enteric infections is a major factor in morbidity and mortality worldwide. an estimated 2-4 billion episodes of infectious diarrhea occur each year and are especially prevalent in infants. this review highlights the cellular and molecular mechanisms underlying diarrhea associated with the three classes of infectious agents, i.e., bacteria, viruses and parasites. several bacterial pathogens have been chosen as model organisms, including vibrio cholerae as a classical example of ... | 2010 | 21327112 |
| frequency of intestinal parasitic infection among children in hadhramout governorate (yemen). | six hundred children from urban and rural regions of hadhramout governorate were examined targeted for the detection of intestinal parasites during the year 2009. the main infective parasites prevailed in children were gardia lamblia (19.17%), entamoeba histolytica (16.83%), ascaris lumbricoides (15.83%), trichuris trichiura (2.33%), hymenolepis nana (2.33%), taenia saginata (1.50%) and schistosoma mansoni (0.67%). besides, infections were accompanied by different symptoms as diarrhea (43.5%), a ... | 2010 | 21246955 |
| intestinal parasites infections in hospitalized aids patients in kinshasa, democratic republic of congo. | to determine the prevalence and the species spectrum of intestinal parasites (ip) involved in hospitalized aids patients, a prospective observational and cross-sectional study was carried out in the four main hospitals in kinshasa, democratic republic of the congo. from november 2006 through september 2007, a single stool sample was collected from 175 hospitalized aids patients older than 15 years. parasites were detected by light microscopy, including ziehl-neelsen, fungi-fluor, modified trichr ... | 2010 | 21275238 |
| the antiretroviral lectin cyanovirin-n targets well-known and novel targets on the surface of entamoeba histolytica trophozoites. | entamoeba histolytica, the protist that causes amebic dysentery and liver abscess, has a truncated asn-linked glycan (n-glycan) precursor composed of seven sugars (man(5)glcnac(2)). here, we show that glycoproteins with unmodified n-glycans are aggregated and capped on the surface of e. histolytica trophozoites by the antiretroviral lectin cyanovirin-n and then replenished from large intracellular pools. cyanovirin-n cocaps the gal/galnac adherence lectin, as well as glycoproteins containing o-p ... | 2010 | 20852023 |
| biophysical characterization of entamoeba histolytica phosphoserine aminotransferase (ehpsat): role of cofactor and domains in stability and subunit assembly. | we investigated the role of the cofactor plp and its binding domain in stability and subunit assembly of phosphoserine aminotransferase (ehpsat) from an enteric human parasite entamoeba histolytica. presence of cofactor influences the tertiary structure of ehpsat because of the significant differences in the tryptophan microenvironment and proteolytic pattern of holo- and apo-enzyme. however, the cofactor does not influence the secondary structure of the enzyme. stability of the protein is signi ... | 2010 | 21161522 |
| epidemiology and control of schistosomiasis and other intestinal parasitic infections among school children in three rural villages of south saint lucia. | the purpose of this study was to determine the epidemiology of parasitic infections and the efficacy of treatment among school children in rural villages of south saint lucia. | 2010 | 21178216 |
| development of a negative selectable marker for entamoeba histolytica. | entamoeba histolytica is the causative agent of amebiasis and infects up to 10% of the world's population. the molecular techniques that have enabled the up- and down-regulation of gene expression rely on the transfection of stably maintained plasmids. while these have increased our understanding of entamoeba virulence factors, the capacity to integrate exogenous dna into genome, which would allow reverse genetics experiments, would be a significant advantage in the study of this parasite. the c ... | 2010 | 21189470 |
| 3d active meshes: fast discrete deformable models for cell tracking in 3d time-lapse microscopy. | variational deformable models have proven over the past decades a high efficiency for segmentation and tracking in 2d sequences. yet, their application to 3d time-lapse images has been hampered by discretization issues, heavy computational loads and lack of proper user visualization and interaction, limiting their use for routine analysis of large data-sets. we propose here to address these limitations by reformulating the problem entirely in the discrete domain using 3d active meshes, which exp ... | 2010 | 21193379 |
| intestinal parasites in immigrants in the city of naples (southern italy). | the present study was aimed at carrying out a cross-sectional copromicroscopic survey of helminths and intestinal protozoa in immigrants in naples (southern italy). between october 2008 and november 2009, a total of 514 immigrants were tested comparing the flotac dual technique and the ethyl acetate concentration technique. combined results of the two techniques served as a diagnostic 'gold' standard and revealed an overall prevalence of parasitic infections of 61.9% (318/514). the ethyl acetate ... | 2010 | 21195044 |
| epigenetics in the unicellular parasite entamoeba histolytica. | amoebiasis is a serious infectious disease that is caused by the unicellular parasite, entamoeba histolytica. this parasite is mainly found in developing countries, and are named owing to its ability to destroy tissues. the molecular mechanisms that regulate the virulence of this parasite are not well understood. in recent years, an increasing interest in the epigenetic regulation of the parasite's virulence has emerged. in this article, an overview of our current knowledge about the role of dna ... | 2010 | 21198420 |
| effect of phosphatidylcholine-cholesterol liposomes on entamoeba histolytica virulence. | trophozoites of entamoeba histolytica hm-1:imss become less virulent after long-term maintenance in axenic cultures. the factors responsible for the loss of virulence during in vitro cultivation remain unclear. however, it is known that in vitro cultivation of amoeba in culture medium supplemented with cholesterol restores their virulence. in this study, we analyzed the effect of adding phosphatidylcholine-cholesterol (pc-chol) liposomes to the culture medium and evaluated the effect of this lip ... | 2010 | 21164568 |
| involvement of src family tyrosine kinase in apoptosis of human neutrophils induced by protozoan parasite entamoeba histolytica. | tyrosine kinases are one of the most important regulators for intracellular signal transduction related to inflammatory responses. however, there are no reports describing the effects of tyrosine kinases on neutrophil apoptosis induced by entamoeba histolytica. in this study, isolated human neutrophils from peripheral blood were incubated with live trophozoites in the presence or absence of tyrosine kinase inhibitors. entamoeba-induced receptor shedding of cd16 and ps externalization in neutroph ... | 2010 | 21234229 |
| rotenoids from lablab purpureus l. and their bioefficacy against human disease vectors. | various plant parts of lablab purpureu s l. were collected and analyzed separately for their rotenoid content. among the plant parts, the maximum content was in the roots and minimum in the seeds. the identity of different rotenoids was confirmed by melting point, mixed melting point, uv, and infrared spectral studies and gas-liquid chromatography. six rotenoids (deguelin, dehydrodeguelin, rotenol, rotenone, tephrosin, and sumatrol) were isolated, identified, and quantified both in vivo and in v ... | 2010 | 20803155 |
| case report: huge amoebic liver abscesses in both lobes. | we describe the case of a patient who returned to china from africa and underwent emergency open surgical drainage with evacuation of 600 ml of anchovy sauce-like fluid from hepatic lesions. computed tomography scans and surgical findings indicated abscesses in both hemilivers and communication between them. bacteriological investigation of the fluid yielded negative results, but dna assay of the pus detected 18s rrna genes of entamoeba histolytica. serum anti-amoebic antibodies were detected us ... | 2010 | 20811141 |
| approaches to characterizing entamoeba histolytica transcriptional regulation. | entamoeba histolytica causes an estimated 100,000 deaths per year and is one of the leading causes of death among parasitic infections. studies using e. histolytica-specific polymerase chain reaction identified that 13.8% of adults in a rural mexican community and 11.2% of adults in central vietnam are asymptomatically colonized. such high incidents of asymptomatic infection suggest that only a minority of infections proceed to invasive disease. understanding the mechanisms that underpin variabl ... | 2010 | 20812994 |
| polymerase chain reaction confirmation of diagnosis of intestinal amebiasis in puducherry. | stool microscopy, the commonly used test for diagnosis of intestinal amebiasis, is unreliable as it does not differentiate entamoeba histolytica, the causative agent for amebiasis, from non-pathogenic entamoeba dispar and entamoeba moshkovskii. | 2010 | 20814775 |
| mass infection with entamoeba histolytica in a japanese institution for individuals with mental retardation: epidemiology and control measures. | after two cases of amoebic colitis were detected at an institution for the mentally retarded in the yamagata prefecture of japan, the prevalence and epidemiology of entamoeba histolytica infection at the institution were investigated. when the 76 residents with mental retardation were checked by serology and stool examinations, 40 (53%) showed evidence of infection with e. histolytica (i.e. e. histolytica-specific antibodies in their serum, entamoeba cysts in their stools, and/or e. histolytica- ... | 2010 | 20819306 |
| intestinal parasites infection among immunocompromised patients in riyadh, saudi arabia. | this study was conducted to determine the prevalence of intestinal parasites among immunocompromised patients in riyadh, saudi arabia. fecal samples were collected from 136 immunocompromised patients (52 females and 84 males), aged 2 to 69 years. specimens were fixed with 10% formalin for 30 min (2-3 g fecal(-1)) and then concentrated by a formalin-ether sedimentation technique. samples were examined as wet saline mounts and in iodine preparation for detection of protozoan oocysts, cysts, helmin ... | 2010 | 20836300 |
| entamoeba histolytica cysteine proteinase 5 binds integrin on colonic cells and stimulates nfkappab-mediated pro-inflammatory responses. | integrins are important mammalian receptors involved in normal cellular functions and the pathogenesis of inflammation and disease. entamoeba histolytica is a protozoan parasite that colonizes the gut, and in 10% of infected individuals, causes amebic colitis and liver abscess resulting in 10(5) deaths/year. e. histolytica-induced host inflammatory responses are critical in the pathogenesis of the disease, yet the host and parasite factors involved in disease are poorly defined. here we show tha ... | 2010 | 20837477 |
| chemical constituents of osyris alba and their antiparasitic activities. | phytochemical investigation of osyris alba l. (santalaceae) of jordanian origin resulted in the isolation and identification of one new pyrrolizidine alkaloid, osyrisine (1), together with 16 other known compounds. the structures of all compounds were established on the basis of spectroscopic analysis. osyrisine, catechin, and catechin-3-o-α-l-rhamnopyranoside exhibited a significant level of antiparasitic activity against two parasites, entamoeba histolytica and giardia intestinalis. | 2010 | 20839131 |
| prevalence of intestinal parasites among school children in northern districts of west bank-palestine. | to assess the prevalence of intestinal parasite infections in northern districts of west bank, palestine and to determine associated sociodemographic factors. | 2010 | 21073639 |
| epidermal cyst infected by entamoeba histolytica in a patient with no past history of intestinal amebiasis. | 2010 | 21091686 | |
| cd4+ and cd8+ t cell- and il-17-mediated protection against entamoeba histolytica induced by a recombinant vaccine. | amebiasis in the murine model can be prevented by vaccination with the gal/galnac lectin through a t cell-dependent mechanism. in this work we further decipher the mechanism of this protection. mice vaccinated with the recombinant "leca" fragment of the gal/galnac lectin with alum were capable of transferring protection to naïve recipients by both cd4+ t cells and surprisingly cd8+ t cells. we then examined the cytokine profile of these cells. cd4+ t cells from pbmc of leca-alum vaccinated mice ... | 2010 | 21095257 |
| calmodulin-like protein from entamoeba histolytica: solution structure and calcium-binding properties of a partially folded protein. | the mechanism of ca(2+)-signaling in the protozoan parasite entamoeba histolytica is yet to be understood as many of the key regulators are still to be identified. e. histolytica encodes a number of multi-ef-hand ca(2+)-binding proteins (ehcabps). functionally only one of these molecules, ehcabp1, has been characterized to date. the calmodulin-like protein from e. histolytica (abbreviated as ehcam or ehcabp3) is a 17.23 kda monomeric protein that shows maximum sequence identity with heterologous ... | 2010 | 21114322 |
| n-acetyl-d-glucosaminylphosphatidylinositol de-n-acetylase from entamoeba histolytica: metal alters catalytic rates but not substrate affinity. | pig-l/gpi12 proteins are endoplasmic reticulum-resident membrane proteins involved in the second step of glycosylphosphatidylinositol anchor biosynthesis in eukaryotes. we show that the entamoeba histolytica pig-l protein is optimally active in the acidic ph range. the enzyme has an intrinsic low level of de-n-acetylase activity in the absence of metal and is significantly stimulated by divalent cations. metal binding induces a large conformational change in the protein that appears to improve c ... | 2010 | 21118807 |
| leptin signaling in intestinal epithelium mediates resistance to enteric infection by entamoeba histolytica. | leptin is an adipocytokine that links nutrition to immunity. previous observation that a genetic polymorphism in the leptin receptor affected susceptibility to entamoeba histolytica infection led to the hypothesis that leptin signaling has a protective role during intestinal amebic infection. in this study we show that mice lacking the functional leptin receptor developed devastating mucosal destruction after e. histolytica infection. bone marrow chimera experiments demonstrated that leptin rece ... | 2010 | 21124310 |
| amebic liver abscess. | 2010 | 21036819 | |
| acute amebic appendicitis: report of a rare case. | acute appendicitis of amebic origin is considered a rare cause of acute appendicitis. we report a case of amebic appendicitis presenting with fever, severe pain in the right lower quadrant of the abdomen and rebound tenderness. lab investigations revealed neutrophilic leukocytosis. the patient underwent appendectomy. histopathological examination revealed numerous entameba histolytica trophozoites in the mucosa of the appendix. acute appendicitis of amebic origin does not appear frequently. appe ... | 2010 | 21045411 |
| [evolution of the prevalence the enteroparasitoses in talca-chile]. | the prevalence of intestinal parasites in preschool and school children in the city of talca and rural areas belonging to the maule region, was assessed annually by means of the serial parasitological studies in stool which were performed in the parasitology laboratory of the "universidad de talca". for consecutive periods since 1980 until 2008, an estimated prevalence of parasitism of 76.2% in the population studied was found. these results show a marked decrease from 9.8% to 2.5% in pathogenic ... | 2010 | 21046719 |
| evaluation of multiplex tandem real-time pcr for detection of cryptosporidium spp., dientamoeba fragilis, entamoeba histolytica, and giardia intestinalis in clinical stool samples. | the aim of this study was to describe the first development and evaluation of a multiplex tandem pcr (mt-pcr) assay for the detection and identification of 4 common pathogenic protozoan parasites, cryptosporidium spp., dientamoeba fragilis, entamoeba histolytica, and giardia intestinalis, from human clinical samples. a total of 472 fecal samples submitted to the department of microbiology at st. vincent's hospital were included in the study. the mt-pcr assay was compared to four real-time pcr (r ... | 2010 | 21048004 |
| in vitro trna methylation assay with the entamoeba histolytica dna and trna methyltransferase dnmt2 (ehmeth) enzyme. | protozoan parasites are among the most devastating infectious agents of humans responsible for a variety of diseases including amebiasis, which is one of the three most common causes of death from parasitic disease. the agent of amebiasis is the amoeba parasite entamoeba histolytica that exists under two stages: the infective cyst found in food or water and the invasive trophozoite living in the intestine. the clinical manifestations of amebiasis range from being asymptomatic to colitis, dysente ... | 2010 | 21048666 |
| synthesis, characterization, antiamoebic activity and cytotoxicity of new pyrazolo[3, 4-d]pyrimidine-6-one derivatives. | a new series of pyrazolo[3,4-d]pyrimidine-6-one derivatives (2a-2j) were prepared by using the biginelli multicomponent cyclocondensation of 3-methyl-1-phenyl-1h-pyrazol-5(4h)-one (1a), different aromatic aldehydes, and urea with a catalytic amount of hcl at reflux temperature. these compounds were characterized by ir, (1)h nmr, (13)c nmr, and mass spectral data. in vitro antiamoebic activity was performed against hm1:imss strain of entamoeba histolytica. the results showed that the compounds 2b ... | 2010 | 21054147 |
| [adaptation of a sensitive dna extraction method for detection of entamoeba histolytica by real-time polymerase chain reaction.] | this study was aimed to adapt a sensitive dna extraction protocol in stool samples for real-time polymerase chain reaction (pcr) detection of entamoeba histolytica which causes important morbidity and mortality worldwide. stool extraction is a problematic step and has direct effects on pcr sensitivity. in order to improve the sensitivity of e.histolytica detection by real-time pcr, "qiaamp dna stool minikit (qiagen, germany)" was modified by adding an overnight incubation step with proteinase k ... | 2010 | 21063995 |
| in vivo visualization of trophozoites in patients with amoebic colitis by using a newly developed endocytoscope. | the endocytoscopy system (ecs) is a new method to provide real-time super-magnifying microscopic imaging in vivo. routine diagnosis of amebic colitis requires multiple tests that are both time consuming and costly. | 2010 | 20579647 |
| downregulation of an entamoeba histolytica rhomboid protease reveals roles in regulating parasite adhesion and phagocytosis. | entamoeba histolytica is a deep-branching eukaryotic pathogen. rhomboid proteases are intramembrane serine proteases, which cleave transmembrane proteins in, or in close proximity to, their transmembrane domain. we have previously shown that e. histolytica contains a single functional rhomboid protease (ehrom1) and has unique substrate specificity. ehrom1 is present on the trophozoite surface and relocalizes to internal vesicles during erythrophagocytosis and to the base of the cap during surfac ... | 2010 | 20581296 |
| entamoeba histolytica liver abscess. | 2010 | 20584930 | |
| an unusual presentation of amebic liver abscesses. | 2010 | 20584933 | |
| infection status of hospitalized diarrheal patients with gastrointestinal protozoa, bacteria, and viruses in the republic of korea. | to understand protozoan, viral, and bacterial infections in diarrheal patients, we analyzed positivity and mixed-infection status with 3 protozoans, 4 viruses, and 10 bacteria in hospitalized diarrheal patients during 2004-2006 in the republic of korea. a total of 76,652 stool samples were collected from 96 hospitals across the nation. the positivity for protozoa, viruses, and bacteria was 129, 1,759, and 1,797 per 10,000 persons, respectively. especially, cryptosporidium parvum was highly mixed ... | 2010 | 20585526 |