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the vibrio parahaemolyticus-infecting bacteriophage qdvp001: genome sequence and endolysin with a modular structure.vibrio parahaemolyticus, a marine pathogen, is a causative agent of gastroenteritis in humans after consumption of contaminated seafood. in recent years, infections with v. parahaemolyticus have become an increasingly frequent factor in microbial food poisoning; therefore, it is urgent to figure out ways to control vibrio parahaemolyticus. endolysins, lytic enzymes encoded by bacteriophages, have been regarded as a therapeutic alternative to antibiotics in control of bacterial growth and have be ...201627376376
differentially expressed transcripts in stomach of penaeus monodon in response to ahpnd infection.acute hepatopancreatic necrosis disease (ahpnd) is an emerging disease in aquacultured shrimp caused by a pathogenic strain of vibrio parahaemolyticus. as with several pathogenic bacteria, colonization of the stomach appeared to be the initial step of the infection for ahpnd-causing vibrio. to understand the immune responses in the stomach of black tiger shrimp (penaeus monodon), differentially expressed transcripts (dets) in the stomach during v. parahaemolyticus strain 3hp (vp3hp) infection wa ...201627339467
report of leucine-rich repeats (lrrs) from scylla serrata: ontogeny, molecular cloning, characterization and expression analysis following ligand stimulation, and upon bacterial and viral infections.leucine-rich repeat (lrr) proteins are present in all living organisms, and their participation in signal transduction and defense mechanisms has been elucidated in humans and mosquitoes. lrrs possibly involve in protein-protein interactions also and show differential expression pattern upon challenge with pathogens. in the present study, a new lrr gene was identified in mud crab, scylla serrata. lrr gene mrna levels in different developmental stages and various tissues of s. serrata were analys ...201627328453
outbreak of vibrio parahaemolyticus sequence type 120, peru, 2009.in 2009, an outbreak of vibrio parahaemolyticus occurred in piura, cajamarca, lambayeque, and lima, peru. whole-genome sequencing of clinical and environmental samples from the outbreak revealed a new v. parahaemolyticus clone. all the isolates identified belonged to a single clonal complex described exclusively in asia before its emergence in peru.201627315090
[transcriptional regulation of hcp1 by h-ns in vibrio parahaemolyticus].to study the transcriptional regulation of the structural components hcp1 by h-ns in vibrio parahaemolyticus.201627305788
vibrio parahaemolyticus strains of pandemic serotypes identified from clinical and environmental samples from jiangsu, china.vibrio parahaemolyticus has emerged as a major foodborne pathogen in china, japan, thailand, and other asian countries. in this study, 72 strains of v. parahaemolyticus were isolated from clinical and environmental samples between 2006 and 2014 in jiangsu, china. the serotypes and six virulence genes including thermostable direct hemolysin (tdr) and tdr-related hemolysin (trh) genes were assessed among the isolates. twenty five serotypes were identified and o3:k6 was one of the dominant serotype ...201627303379
transcription of exsd is repressed directly by h-ns in vibrio parahaemolyticus.vibrio parahaemolyticus is a leading cause of seafood-associated diarrhea and gastroenteritis. this bacteria expresses a major virulence determinant called t3ss1. expression of t3ss1 is tightly regulated by the exsa-exsc-exsd regulatory system. the transcription of exsa and probably exsc is repressed directly by the h-ns protein. in this study, the regulation of exsd by h-ns was investigated using quantitative rt-pcr, primer extension, lacz fusion, electrophoretic mobility shift, and dnase i foo ...201627289037
characterization of adp ribosylation factor 1 gene from exopalaemon carinicauda and its immune response to pathogens challenge and ammonia-n stress.adp ribosylation factors (arf), as highly conserved small guanosine triphosphate (gtp)-binding proteins, participates in intracellular trafficking and organelle structure. in this study, a full-length cdna of arf1 (designated ecarf1) was cloned from exopalaemon carinicauda by using rapid amplification of cdna ends (race) approaches. the full-length cdna of ecarf1 was 1428 bp, which contains an open reading frame (orf) of 549 bp, encoding a 182 amino-acid polypeptide with the predicted molecular ...201627231192
closing the brief case: safe to go back in the water? vibrio parahaemolyticus wound infection associated with brackish water. 201627217388
effects of intertidal harvest practices on levels of vibrio parahaemolyticus and vibrio vulnificus bacteria in oysters.vibrio parahaemolyticus and vibrio vulnificus can grow rapidly in shellfish subjected to ambient air conditions, such as during intertidal exposure. in this study, levels of total and pathogenic (tdh(+) and/or trh(+)) v. parahaemolyticus and total v. vulnificus were determined in oysters collected from two study locations where intertidal harvest practices are common. samples were collected directly off intertidal flats, after exposure (ambient air [washington state] or refrigerated [new jersey] ...201627208133
photodynamic effect of curcumin on vibrio parahaemolyticus.vibrio parahaemolyticus (v. parahaemolyticus) is currently a major cause of bacterial diarrhoea associated with seafood consumption. the objective of this study was to determine the inactivation effect of curcumin-mediated photodynamic action on v. parahaemolyticus. first of all, v. parahaemolyticus suspended in pbs buffer was irradiated by a visible light from a led light source with an energy density of 3.6j/cm(2). colony forming units (cfu) were counted and the viability of v. parahaemolyticu ...201627177810
complete genome sequence of vibrio parahaemolyticus strain forc_008, a foodborne pathogen from a flounder fish in south korea.vibrio parahaemolyticus is a gram-negative, motile, nonspore-forming pathogen that causes foodborne illness associated with the consumption of contaminated seafoods. although many cases of foodborne outbreaks caused by v. parahaemolyticus have been reported, the genomes of only five strains have been completely sequenced and analyzed using bioinformatics. in order to characterize overall virulence factors and pathogenesis of v. parahaemolyticus associated with foodborne outbreak in south korea, ...201627170457
population relationship of vibrio parahaemolyticus isolates derived from aquaculture ponds, a seafood market, restaurants, and clinical samples.to study the relationship between environmental and clinical populations of vibrio parahaemolyticus, we collected in total 86 isolates from southern china during one and a half years. sixty-eight isolates were recovered from aquaculture ponds, a seafood market, and restaurants, and 18 isolates were recovered from clinical samples. virulence gene analysis revealed that 25 isolates (14 clinical and 11 environmental) tested positive for tdh, but only 4 carried trh. interestingly, none of the tdh(+) ...201627166752
sero-prevalence and genetic diversity of pandemic v. parahaemolyticus strains occurring at a global scale.pandemic vibrio parahaemolyticus is an emerging public health concern as it has caused numerous gastroenteritis outbreaks worldwide. currently, the absence of a global overview of the phenotypic and molecular characteristics of pandemic strains restricts our overall understanding of these strains, especially for environmental strains. to generate a global picture of the sero-prevalence and genetic diversity of pandemic v. parahaemolyticus, pandemic isolates from worldwide collections were select ...201627148244
prevalence, molecular characterization, and antibiotic susceptibility of vibrio parahaemolyticus from ready-to-eat foods in china.vibrio parahaemolyticus is the leading cause of foodborne outbreaks, particularly outbreaks associated with consumption of fish and shellfish, and represents a major threat to human health worldwide. this bacterium harbors two main virulence factors: the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh). additionally, various serotypes have been identified. the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant v. paraha ...201627148231
environmental conditions associated with elevated vibrio parahaemolyticus concentrations in great bay estuary, new hampshire.reports from state health departments and the centers for disease control and prevention indicate that the annual number of reported human vibriosis cases in new england has increased in the past decade. concurrently, there has been a shift in both the spatial distribution and seasonal detection of vibrio spp. throughout the region based on limited monitoring data. to determine environmental factors that may underlie these emerging conditions, this study focuses on a long-term database of vibrio ...201627144925
b-cell activating cpg odn 1668 enhance the immune response of pacific red snapper (lutjanus peru) exposed to vibrio parahaemolitycus.b-class cpg odn 1668 is known to possess clear immunostimulatory properties. in this study, we investigated the potential ability of cpg odn 1668 to enhance the immune response of pacific red snapper exposed to vibrio parahaemolyticus. four different treatments were evaluated in pacific red snapper: (1) stimulatory cpg odn 1668, (2) stimulatory cpg odn 1668 and v. parahaemolyticus, (3) exposure only to v. parahaemolyticus and (4) pbs. samples were taken at 24, 72, 168 and 240 h of stimulation/in ...201627143535
incidence and prevalence of vibrio parahaemolyticus in seafood: a systematic review and meta-analysis.vibrio parahaemolyticus is an important seafood borne human pathogen worldwide due to it occurrence, prevalence and ability to cause gastrointestinal infections. this current study aim at investigating the incidence and prevalence of v. parahaemolyticus in seafood using systematic review-meta-analysis by exploring heterogeneity among primary studies. a comprehensive systematic review and meta-analysis of peer reviewed primary studies reported between 2003 and 2015 for the occurrence and prevalen ...201627119068
metabolome response to temperature-induced virulence gene expression in two genotypes of pathogenic vibrio parahaemolyticus.vibrio parahaemolyticus is a main causative agent of serious human seafood-borne gastroenteritis disease. many researchers have investigated its pathogenesis by observing the alteration of its virulence factors in different conditions. it was previously known that culture conditions will influence the gene expression and the metabolic profile of v. parahaemolyticus, but little attention has been paid on the relationship between them. in this study, for the first time, the metabolomics response i ...201627113578
molecular characterizations of vibrio parahaemolyticus in seafood from the black sea, turkey.vibrio parahaemolyticus is a marine bacterium that is considered as one of the major causes of bacterial food-borne outbreaks at a global scale. a total of 114 samples including mussel (n = 42), seawater (n = 22) and fish (n = 50) samples were collected and subjected to investigation. vibrio parahaemolyticus was detected in 45 (39%) of 114 samples with an occurrence in mussel, seawater and fish samples of 76, 40·9 and 8% respectively. a total of 96 isolates were positive for the species-specific ...201627107401
the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus: sequence variation and implications for detection and function.vibrio parahaemolyticus is a leading cause of bacterial food-related illness associated with the consumption of undercooked seafood. only a small subset of strains is pathogenic. most clinical strains encode for the thermostable direct hemolysin (tdh) and/or the tdh-related hemolysin (trh). in this work, we amplify and sequence the trh gene from over 80 trh+strains of this bacterium and identify thirteen genetically distinct alleles, most of which have not been deposited in genbank previously. s ...201627094247
antibiotic and heavy-metal resistance of vibrio parahaemolyticus isolated from fresh shrimps in shanghai fish markets, china.vibrio parahaemolyticus is a causative agent of human serious seafood-borne gastroenteritis disease and even death. shrimps, often eaten raw or undercooked, are an important reservoir of the bacterium. in this study, we isolated and characterized a total of 400 v. parahaemolyticus strains from commonly consumed fresh shrimps (litopenaeus vannamei, macrobrachium rosenbergii, penaeus monodon, and exopalaemon carinicauda) in shanghai fish markets, china in 2013-2014. the results revealed an extreme ...201627083906
draft genome sequence of an environmental trh+ vibrio parahaemolyticus k23 strain isolated from kerala, india.vibrio parahaemolyticusis the leading cause of seafood-related gastroenteritis. here, we report the draft genome sequence of atrh(+)strain,v. parahaemolyticusk23, isolated from seafood. the sequence will be useful for comparative analysis between environmental and clinical isolates ofv. parahaemolyticus.201627081143
immunoproteomic approach for screening vaccine candidates from bacterial outer membrane proteins.outer membrane proteins (omps) are unique to gram-negative bacteria and have been revealed as potential vaccine candidates for conferring protection against infections in recent years. immunoproteomics is a powerful technique that is ideally suited to screen and identify potential vaccine candidates. this chapter presents a brief outline of the screening of immunogenic omps from vibrio parahaemolyticus by an immunoproteomic strategy that was based on two-dimensional electrophoresis (2-de) and im ...201627076320
complete genome sequence of vibrio parahaemolyticus forc_023 isolated from raw fish storage water.vibrio parahaemolyticusis a gram-negative halophilic bacterium that causes food-borne gastroenteritis in humans who consumev. parahaemolyticus-contaminated seafood.the forc_023 strain was isolated from raw fish storage water, containing live fish at a sashimi restaurant. here, we aimed to sequence and characterize the genome of the forc_023 strain. the genome of the forc_023 strain showed two circular chromosomes, which contained 4227 open reading frames (orfs), 131 trna genes and 37 rrna genes. ...201627073252
major tdh(+)vibrio parahaemolyticus serotype changes temporally in the bay of bengal estuary of bangladesh.vibrio parahaemolyticus is responsible for seafood-related gastroenteritis worldwide. in bangladesh, diarrhea is endemic and diarrheagenic v. parahaemolyticus serotypes occur naturally in the coastal and estuarine aquatic environment. v. parahaemolyticus strains, isolated from estuarine surface water of the bay of bengal villages of bangladesh during 2006-2008, were tested for the presence of virulence and pandemic-marker genes, serodiversity, and phylogenetic relatedness. pcr analysis of v. par ...201627063395
occurrence of pathogenic vibrio parahaemolyticus in crustacean shellfishes in coastal parts of eastern india.the objective of the study was to isolate and characterize pathogenic vibrio parahaemolyticus from crustacean shellfishes (crab and shrimp) commonly retailed in coastal parts of eastern india.201627057121
antibiotic resistance of vibrio parahaemolyticus and vibrio vulnificus in various countries: a review.vibrio parahaemolyticus and vibrio vulnificus are the leading causes of seafood associated infections and mortality in the united states. the main syndromes caused by these pathogens are gastroenteritis, wound infections, and septicemia. this article reviewed the antibiotic resistance profile of v. parahaemolyticus and v. vulnificus in the united states and other countries including italy, brazil, philippines, malaysia, thailand, china, india, iran, south africa and australia. the awareness of a ...201627052711
combined treatment of uva irradiation and antibiotics induces greater bactericidal effects on vibrio parahaemolyticus.the presence of antibiotics in the environment and their subsequent impact on the development of multi-antibiotic resistant bacteria has raised concerns globally. consequently, much research is focused on a method to produce a better disinfectant. we have established a disinfectant system using uva-led that inactivates pathogenic bacteria. we assessed the bactericidal efficiency of a combination of uva-led and antibiotics against vibrio parahaemolyticus. combined use of antibiotic drugs and uva ...201627040055
dietary pyridoxine protects against stress and maintains immunohaematological status in chanos chanos exposed to endosulfan.the amelioration effect of water-soluble vitamin pyridoxine against stress was evaluated in milkfish, chanos chanos exposed to endosulfan. two hundred and twenty-five fish were distributed randomly into five treatments, each with three replicates. four isocaloric and isonitrogenous diets with graded levels of pyridoxine feed were as follows: normal water and fed with control diet (en0/py0); endosulfan-treated water and fed with control diet (en/py0); and endosulfan-treated water and fed with 50 ...201627038044
molecular epidemiology of selected infectious diseases caused by bacteria in juveniles and post-larvae of the white shrimp penaeus vannamei from the north-western coast of mexico. 201627037647
cloning, identification and functional analysis of a β-catenin homologue from pacific white shrimp, litopenaeus vannamei.wnt signaling is known to control multiple of cellular processes such as cell differentiation, communication, apoptosis and proliferation, and is also reported to play a role during microbial infection. β-catenin is a key regulator of the wnt signaling cascade. in the present study, we cloned and identified a β-catenin homologue from litopenaeus vannamei termed lvβ-catenin. the full-length of lvβ-catenin transcript was 2797 bp in length within a 2451 bp open reading frame (orf) that encoded a pr ...201627036405
the master quorum-sensing regulator opar is activated indirectly by h-ns in vibrio parahaemolyticus.apha and opar are the master regulators of quorum sensing in vibrio parahaemolyticus and there is reciprocal negative regulation between apha and opar. the histone-like nucleoid structure (h-ns) protein is a global transcriptional repressor of horizontally transferred genes, but a few prokaryotic genes, for example flagella genes, are stimulated by h-ns. the regulation of opar by h-ns was investigated using the following methods: primer extension, lacz fusion, quantitative rt-pcr, and electropho ...201627025725
a nonautochthonous u.s. strain of vibrio parahaemolyticus isolated from chesapeake bay oysters caused the outbreak in maryland in 2010.in the summer of 2010, vibrio parahaemolyticus caused an outbreak in maryland linked to the consumption of oysters. strains isolated from both stool and oyster samples were indistinguishable by pulsed-field gel electrophoresis (pfge). however, the oysters contained other potentially pathogenic v. parahaemolyticus strains exhibiting different pfge patterns. in order to assess the identity, genetic makeup, relatedness, and potential pathogenicity of the v. parahaemolyticus strains, we sequenced 11 ...201626994080
function of gc1qr in innate immunity of chinese mitten crab, eriocheir sinensis.gc1qr is identified as the globular "head" binding protein of the c1q protein and performs an important function in innate immunity. a esgc1qr gene was identified from the hepatopancreas of eriocheir sinensis. esgc1qr encodes a protein with 275 amino acids. phylogenetic analysis showed that, together with crustaceans gc1qrs, esgc1qr belongs to one group. esgc1qr mrna was detected in hemocytes, intestine, hepatopancreas, gills, eyestalk, heart, muscle, and nerve. the expression of the esgc1qr tra ...201626993663
inactivation of vibrio parahaemolyticus unattached and attached to a solid surface in ph-controlled sodium hypochlorite solutions.the inactivation of vibrio parahaemolyticus cells that were unattached or attached to a polyethylene terephthalate (pet) disc in ph-controlled sodium hypochlorite (naocl) solutions was studied under turbulent conditions. no significant desorption of attached cells occurred at the free available chlorine (fac) concentrations from 0.1 to 1.0 mg/l. the number of viable cells was estimated by microbial calorimetry. the logarithmic relative reduction of viable cells was proportional to the product of ...201628003634
actin cytoskeleton-modulating t3ss2 effectors and their contribution to the vibrio parahaemolyticus-induced diarrhea.to understand how bacterial pathogens cause diseases is the most important step in order to prevent the infection and develop an effective treatment. however, the past proceeding studies make us aware of quite-complicated interactions between the host and pathogenic bacteria. vibrio parahaemolyticus, a food-born pathogen that is a subject of our study, causes inflammatory diarrhea in human upon ingestion of contaminated raw or undercooked seafood. many virulence factors has been proposed since i ...201627980291
first multi-year retrospective study on vibrio parhaemolyticus and vibrio vulnificus prevalence in ruditapes philippinarum harvested in sacca di goro, italy.the present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of vibrio spp., and the prevalence of v. parahaemolyticus and v. vulnificus in the manila clam r. philippinarum harvested in sacca di goro, emilia-romagna region, northern italy. on the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have ...201628058248
development of a more sensitive and specific chromogenic agar medium for the detection of vibrio parahaemolyticus and other vibrio species.foodborne infections in the us caused by vibrio species have shown an upward trend. in the genus vibrio, v. parahaemolyticus is responsible for the majority of vibrio-associated infections. thus, accurate differentiation among vibrio spp. and detection of v. parahaemolyticus is critically important to ensure the safety of our food supply. although molecular techniques are increasingly common, culture-depending methods are still routinely done and they are considered standard methods in certain c ...201627911360
evaluation of medicinal plants and colloidal silver efficiency against vibrio parahaemolyticus infection in litopenaeus vannamei cultured at low salinity.in shrimp aquaculture, reduction in the use of synthetic antibiotics is a priority due to the high incidence of resistant bacteria (vibrio) in the white shrimp litopenaeus vannamei. an increasing number of studies show bactericidal activity of natural treatments in aquaculture. the effectiveness of neem (azadirachta indica) and oregano (lippia berlandieri) aqueous extracts and colloidal silver against v. parahaemolyticus were evaluated in low salinity shrimp culture. results show that aqueous ex ...201627901504
pi3k-akt signaling pathway is involved in hypoxia/thermal-induced immunosuppression of small abalone haliotis diversicolor.the pi3k-akt signal pathway has been found to be involved in many important physiological and pathological processes of the innate immune system of vertebrates and invertebrates. in this study, the akt (hdakt) and pi3k (hdpi3k) gene of small abalone haliotis diversicolor were cloned and characterized for the important status of pi3k and akt protein in pi3k-akt signaling pathway. the full length cdnas of hdakt and hdpi3k are 2126 bp and 6052 bp respectively, encoding proteins of 479 amino acids a ...201627825946
a c-type lectin (mrlec) with high expression in intestine is involved in innate immune response of macrobrachium rosenbergii.c-type lectins (ctls) are pattern-recognition proteins that play an important role in innate immunity of vertebrates and invertebrates. in this study, a lectin cdna named mrlec was cloned and characterized from giant freshwater prawns (macrobrachiun rosenbergii). the full-length cdna of mrlec was 1431 bp, which contained an open reading frame of 1041 bp that encoded a protein with 346 amino acids. mrlec was found to contain a typical signal peptide of 18 amino acids and a single carbohydrate-rec ...201627818342
in vivo activity and the transcriptional regulatory mechanism of the antimicrobial peptide sphyastatin in scylla paramamosain.a new gene homologous to the reported antimicrobial peptide (amp) hyastatin from hyas araneus was screened in the ssh library constructed from the hemocytes of scylla paramamosain, and named sphyastatin. in vivo study showed that sphyastatin was predominantly expressed in hemocytes of s. paramamosain. with the challenge of either vibrio parahaemolyticus or lipopolysaccharide (lps), sphyastatin showed a positive response, meaning that it was probably involved in the immune reaction against bacter ...201627751917
virulence of acute hepatopancreatic necrosis disease pirab-like relies on secreted proteins not on gene copy number.to investigate the virulence of the vp_pirab-like genes in vibrio parahaemolyticus- acute hepatopancreatic necrosis disease (ahpnd)-causing strain and the factors that are associated with the virulence level.201627522063
regulation of the expression of iron-acquisition system genes in pathogenic vibrio species.the genus vibrio includes >70 species, of which roughly a dozen cause vibriosis such as gastroenteritis, wound infections, and septicemia. most bacteria, including vibrio species, require iron for survival and growth. however, the bioavailability of iron is extremely low because it is usually present as an insoluble ferric complex in an aerobic environment or is bound to iron-binding proteins in mammalian hosts. therefore many bacteria have developed iron acquisition systems, including biosynthe ...201627803484
pharmacokinetic indices for cefovecin after single-dose administration to adult sea otters (enhydra lutris).seven sea otters received a single subcutaneous dose of cefovecin at 8 mg/kg body weight. plasma samples were collected at predetermined time points and assayed for total cefovecin concentrations using ultra-performance liquid chromatography and tandem mass spectrometry. the mean (±sd) noncompartmental pharmacokinetic indices were as follows: cmax (obs) 70.6 ± 14.6 μg/ml, tmax (obs) 2.9 ± 1.5 h, elimination rate constant (kel ) 0.017 ± 0.002/h, elimination half-life (t1/2kel) 41.6 ± 4.7 h, area ...201627212054
passive immune-protection of litopenaeus vannamei against vibrio harveyi and vibrio parahaemolyticus infections with anti-vibrio egg yolk (igy)-encapsulated feed.vibrio spp. are major causes of mortality in white shrimp (litopenaeus vannamei) which is lacking adaptive immunity. passive immunization with a specific egg yolk antibody (igy) is a potential method for the protection of shrimp against vibriosis. in this study, immune effects of the specific egg yolk powders (igy) against both v. harveyi and v. parahaemolyticus on white shrimp were evaluated. the egg yolk powders against v. harveyi and v. parahaemolyticus for passive immunization of white shrim ...201627196895
detection and antimicrobial resistance of vibrio isolates in aquaculture environments: implications for public health.the aim of this study was to evaluate the presence of vibrio isolates recovered from four different fish pond facilities in benin city, nigeria, determine their antibiogram profiles, and evaluate the public health implications of these findings. fish pond water samples were collected from four sampling sites between march and september 2014. a total of 56 samples were collected and screened for the isolation of vibrio species using standard culture-based methods. polymerase chain reaction (pcr) ...201626540391
differential proteomics to explore the inhibitory effects of acidic, slightly acidic electrolysed water and sodium hypochlorite solution on vibrio parahaemolyticus.slightly acidic electrolysed water (slaew) and acidic electrolysed water (aew) have been demonstrated to effectively inactivate food-borne pathogens. however, the underlying mechanism of inactivation remains unknown. therefore, in this study, a differential proteomic platform was used to investigate the bactericidal mechanism of slaew, aew, and sodium hypochlorite (naocl) solutions against vibrio parahaemolyticus. the upregulated proteins after slaew, aew, and naocl treatments were identified as ...201626471589
halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality.predation on bacteria and accompanying mortality are important mechanisms in controlling bacterial populations and recycling of nutrients through the microbial loop. the agents most investigated and seen as responsible for bacterial mortality are viruses and protists. however, a body of evidence suggests that predatory bacteria such as the halobacteriovorax (formerly bacteriovorax), a bdellovibrio-like organism, contribute substantially to bacterial death. until now, conclusive evidence has been ...201626251870
centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection.we present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (lamp) and colorimetric detection using eriochrome black t (ebt). five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. the sequential loading and aliquoting of the lamp cocktail, the primer mixtures, and the dna sample solutions were accomplishe ...201626322592
zebrafish as a useful model for zoonotic vibrio parahaemolyticus pathogenicity in fish and human.vibrio parahaemolyticus is an important aquatic zoonotic pathogen worldwide that causes vibriosis in many marine fish, and sepsis, gastroenteritis and wound infection in humans. however, the pathogenesis of different sources of v. parahaemolyticus is not fully understood. here, we examined the pathogenicity and histopathology of fish (v. parahaemolyticus 1.2164) and human (v. parahaemolyticus 17) strains in a zebrafish (danio rerio). we found that different infection routes resulted in different ...201626519599
prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from oysters in korea.vibrio parahaemolyticus is the most prevalent gastroenteritis-causing pathogen in korea and in some other asian countries. it is frequently found in oysters and other seafood. this study monitored changes in the prevalence of v. parahaemolyticus and environmental parameters in oyster aquaculture environments in korea. from june to october 2014, we tested oysters (crassostrea gigas) from shellfish-harvesting areas off the west coast of korea. these 71 isolates were the sum of 16 (22.5%), 19 (26.8 ...201626511256
detection, identification, and prevalence of pathogenic vibrio parahaemolyticus in fish and coastal environment in jordan.vibrio parahaemolyticus is widely distributed in the marine environments and considered the leading cause of human gastroenteritis in asian countries. a total of 150 marketed fish and 50 water and sediment samples from the gulf of aqaba were examined for the prevalence of pathogenic strains of v. parahaemolyticus. a total of 132 typical isolates obtained from the primary selective medium (thiosulfate-citrate bile salt sucrose agar) and showed positive biochemical properties were subjected to con ...201626554333
epidemiological investigation of a foodborne outbreak in spain associated with u.s. west coast genotypes of vibrio parahaemolyticus.we describe an outbreak of seafood-associated vibrio parahaemolyticus in galicia, spain in on 18th of august 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. the food items identified through the questionnaires as the most probable source of the infections was shrimp. this product was unique in showing a statistically significant and ...201626848427
identification of immune response-related genes and signalling pathways in spleen of vibrio parahaemolyticus-infected epinephelus fuscoguttatus (forskal) by next-generation sequencing. 201625786532
inactivation of vibrio parahaemolyticus by antimicrobial photodynamic technology using methylene blue.vibrio parahaemolyticus is the leading causative pathogen of gastroenteritis often related to contaminated seafood. photodynamic inactivation has been recently proposed as a strategy for killing cells and viruses. the objective of this study was to verify the bactericidal effects caused by photodynamic inactivation using methylene blue (mb) over v. parahaemolyticus via flow cytometry, agarose gel electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis. vibrio parahaemolytic ...201625989459
detection of acute hepatopancreatic necrosis disease strain of vibrio parahaemolyticus using loop-mediated isothermal amplification. 201625988949
the first report of vibrio parahaemolyticus strain o10:k60 in japan, a new combination of o and k serotypes isolated from a patient with gastroenteritis.vibrio parahaemolyticus is an important pathogen that causes gastroenteritis in humans, generally associated with the consumption of contaminated seafood, particularly raw shellfish. there are many serotypes in v. parahaemolyticus resulting from a combination of o and k antigens. among them, o3:k6 and their variants, which represent the pandemic clone, are the most widespread strains worldwide. in this study, we examined v. parahaemolyticus isolated from a gastroenteritis patient's stool at a ho ...201625971326
preparation, characterization and antibacterial activity of chitosan and phosphorylated chitosan from cuttlebone of sepia kobiensis (hoyle, 1885).chitosan is a commercially available derivative of chitin that has been extensively studied for its antimicrobial properties. in order to improve the water solubility and its biological activity, the chemical modification or derivatisation is attempted. in the present investigation, the chitosan prepared from the cuttlebone of sepia kobiensis was being chemically modified by reacting it with orthophosphoric acid so as to obtain phosphorylated chitosan. then the chitosan and phosphorylated chitos ...201628507912
identification and characterization of a laccase from litopenaeus vannamei involved in anti-bacterial host defense.phenoloxidases (pos) are a family of enzymes including tyrosinases, catecholases and laccases, which play an important role in immune defences of various invertebrates. whether or not laccase exists in shrimp and its function is still poorly understood. in this study, a laccase (lvlac) was cloned and identified from litopenaeus vannamei for the first time. the full length of lvlac is 3406 bp, including a 2034 bp open reading frame (orf) coding for a putative protein of 677 amino acids with a sig ...201728476665
identification of a macrobrachium nipponense c-type lectin with a close evolutionary relationship to vertebrate lectins.c-type lectins (ctls) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. this study cloned and characterized a novel c-type lectin (mnctl) of the oriental river prawn, macrobrachium nipponense. the cloned mnctl cdna encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. the deduced mnctl protein contains a single carbohydrate recognition domain (crd) with an epn (glu-pro-asn) motif in calcium-binding site ...201728441623
vibrio parahaemolyticus- and v. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (tursiops truncatus) from the adriatic coast of italy.a case of vibrio parahaemolyticus- and v. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (tursiops truncatus) found stranded along the adriatic coast of italy in 2016 is herein reported, along with a minireview on v. parahaemolyticus and v. alginolyticus infections in aquatic mammals. macroscopically, two abscesses were found in the dolphin's forebrain, along with an extensive, bilateral, parasitic broncho-pneumonia. histologically, a suppurative-to-pyogranulomatous mening ...201728709108
previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora.a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ...201728842846
corrigendum: assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis.[this corrects the article on p. 760 in vol. 6, pmid: 26284045.].201728450861
a single wap domain-containing protein from litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against ahpnd-inducing vibrio parahaemolyticus.the single wap domain-containing protein (swd) is a type iii crustin antimicrobial peptide whose function is to defense the host animal against the bacterial infection by means of antimicrobial and antiproteinase activities. a study of swd from litopenaeus vannamei (lvswd) is reported herein about its activities and function against bacteria, particularly the ahpnd-inducing vibrio parahaemolyticus (vpahpnd) that causes acute hepatopancreatic necrosis disease (ahpnd). the lvswd is mainly synthesi ...201728743625
bioluminescence imaging to track bacteroides fragilis inhibition of vibrio parahaemolyticus infection in mice.bacteroides fragilis is an anaerobic, gram-negative, commensal bacterium of the human gut. it plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. many recent studies have focused on the relationship between b. fragilis and human immunity, and indicate that b. fragilis has many useful probiotic effects. as inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether b. fragil ...201728553617
whole-body microbiota of sea cucumber (apostichopus japonicus) from south korea for improved seafood management.sea cucumber (apostichopus japonicus) is a popular seafood source in asia, including south korea, and its consumption has recently increased with recognition of its medicinal properties. however, because raw sea cucumber contains various microbes, its ingestion can cause foodborne illness. therefore, analysis of the microbiota in the whole body of sea cucumber can extend our understanding of foodborne illness caused by microorganisms and help to better manage products. we collected 40 sea cucumb ...201728851208
role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties.β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ...201728684323
expression of a vibrio parahaemolyticus toxin in escherichia coli results in chromosomal dna degradation.a toxin-antitoxin system, vp1842/vp1843, locates within a superintegron on the vibrio parahaemolyticus genome chromosome i whose toxin gene vp1843 encodes a dna nicking endonuclease. we found that the vp1843 expression in escherichia coli cells strongly induced chromosomal dna degradation. on the basis of these observations, we discuss a possible physiological role of vp1842/vp1843 in v. parahaemolyticus.201728820009
ecofriendly synthesis of silver and gold nanoparticles by euphrasia officinalis leaf extract and its biomedical applications.biogenic synthesis of silver (agnps) and gold nanoparticles (aunps) using aqueous extract of euphrasia officinalis has been reported. stable agnps and aunps were formed on adding aqueous solutions of silver nitrate and chloroauric acid with e. officinalis leaf extract, in 19 min and 2 min, respectively. the synthesis method used in present study was simple, reliable, rapid, cost effective and ecofriendly. the synthesized nanoparticles were characterized with field emission transmission electron ...201728784039
seasonal influence of scallop culture on nutrient flux, bacterial pathogens and bacterioplankton diversity across estuaries off the bohai sea coast of northern china.in this study, we investigated the environmental impacts of scallop culture on two coastal estuaries adjacent the bohai sea including developing a quantitative pcr assay to assess the abundance of the bacterial pathogens escherichia coli and vibrio parahaemolyticus. scallop culture resulted in a significant reduction of nitrogen, chlorophyll a, and phosphorous levels in seawater during summer. the abundance of bacteria including v. parahaemolyticus varied significantly across estuaries and breed ...201728779889
removal of foodborne pathogen biofilms by acidic electrolyzed water.biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (eps), are difficult to remove in food production facilities. in this study, the use of acidic electrolyzed water (aew) to remove foodborne pathogen biofilms was evaluated. we used a green fluorescent protein-tagged escherichia coli for monitoring the efficiency of aew for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm di ...201728638370
a rele/pare superfamily toxin in vibrio parahaemolyticus has dna nicking endonuclease activity.type ii toxins in toxin-antitoxin (ta) systems fold into a similar fold and belong to the rele/pare superfamily. however, they display two distinct biochemical activities: rele toxins are mrna interferases, while pare toxins are dna gyrase (gyr) inhibitors. previously, we found a ta system, vp1842/vp1843, on the vibrio parahaemolyticus genome whose toxin vp1843 belongs to the rele/pare toxin superfamily. vp1843, unlike rele toxins, has neither protein synthesis inhibitory activity nor ribonuclea ...201728533087
molecular characteristics, biofilm-forming abilities, and quorum sensing molecules in vibrio parahaemolyticus strains isolated from marine and clinical environments in korea.vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. in this study, an ultrafast labchip real-time pcr assay was evaluated for rapid detection and quantification of pathogenic v. parahaemolyticus isolates. escherichia coli and vibrio harveyi were used as negative controls. twenty-six tdh-positive, biofilm-producing v. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction ...201728452235
application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review.electrolysed oxidising water (e.o. water) is produced by electrolysis of sodium chloride to yield primarily chlorine based oxidising products. at neutral ph this results in hypochlorous acid in the un-protonated form which has the greatest oxidising potential and ability to penetrate microbial cell walls to disrupt the cell membranes. e.o. water has been shown to be an effective method to reduce microbial contamination on food processing surfaces. the efficacy of e.o. water against pathogenic ba ...201728416883
groel is a suitable genetic marker for detecting vibrio parahaemolyticus by loop-mediated isothermal amplification assay.a groel gene-based loop-mediated isothermal amplification (lamp) assay was developed to detect vibrio parahaemolyticus in contaminated seafood and water. the assay was optimized and conducted at 63°c for 40 min using bacillus stearothermophilus (bst) dna polymerase, large fragment. amplification was analysed via multiple detection methods, including opacity, formation of white precipitate, dna intercalating dyes (ethidium bromide and sybr green i), metal ion-binding indicator dye, calcein, and 2 ...201728585379
purification and antibacterial mechanism of fish-borne bacteriocin and its application in shrimp (penaeus vannamei) for inhibiting vibrio parahaemolyticus.vibrio parahaemolyticus: is recognized as the main cause of gastroenteritis associated with consumption of seafood. bacteriocin-producing lactobacillus plantarum fgc-12 isolated from golden carp intestine had strong antibacterial activity toward v. parahaemolyticus. the fish-borne bacteriocin was purified by a three-step procedure consisting of ethyl acetate extraction, gel filtration chromatography and high performance liquid chromatography. its molecular weight was estimated at 4.1 kda using s ...201728702797
molecular identification of disk abalone (haliotis discus discus) tetraspanin 33 and cd63: insights into potent players in the disk abalone host defense system.tetraspanins are a superfamily of transmembrane proteins involved in a diverse range of physiological processes including differentiation, adhesion, signal transduction, cell motility, and immune responses. in the present study, two tetraspanins, cd63 and tetraspanin 33 (tspan33) from disk abalone (abcd63 and abtspan33), were identified and characterized at the molecular level. the coding sequences for abcd63 and abtspan33 encoded polypeptides of 234 and 290 amino acids (aa) with predicted molec ...201728823981
a cysteine protease (cathepsin z) from disk abalone, haliotis discus discus: genomic characterization and transcriptional profiling during bacterial infections.cathepsin z (ctsz) is lysosomal cysteine protease of the papain superfamily. it participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. hence, ctsz is also acknowledged as an acute-phase protein in host immunity. in this study, we sought to identify the ctsz homolog from disk abalone (abctsz) and characterize it at the molecular, genomic, and transcrip ...201728687334
new chlorinated xanthone and anthraquinone produced by a mangrove-derived fungus penicillium citrinum hl-5126.two new chlorinated metabolites 4-chloro-1-hydroxy-3-methoxy-6-methyl-8-methoxycarbonyl-xanthen-9-one (1) and 2'-acetoxy-7-chlorocitreorosein (2), together with three known compounds (3-5), were obtained from the etoac extract of the endophytic fungus penicillium citrinum hl-5126 isolated from the mangrove bruguiera sexangula var. rhynchopetala collected in the south china sea. their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. all compounds were evalu ...201728465625
antibacterial activities and preservative effect of chitosan oligosaccharide maillard reaction products on penaeus vannamei.the purpose of this study was to understand the influence of maillard reaction (mr) on bioactivity of chitosan oligosaccharide (cos). the antibacterial activity against pseudomonas aeruginosa and vibrio parahaemolyticus and preservative effect of two kinds of cos mr products (mrps) on penaeus vannamei were evaluated. the structures of cg (mrps of cos and glucose) and cm (mrps of cos and maltose) were characterized by ft-ir and their molecular weights were measured by gel permeation chromatograph ...201728732733
a c1qdc protein (hcc1qdc6) with three tandem c1q domains is involved in immune response of triangle-shell pearl mussel (hyriopsis cumingii).c1q-domain-containing (c1qdc) proteins are a family of proteins with a globular c1q (gc1q) domain and participate in several immune responses. in this study, a c1qdc gene was identified from the triangle-shell pearl mussel hyriopsis cumingii (designated as hcc1qdc6). this gene has a full-length cdna of 1782 bp and an open reading frame of 1,335 bp that encodes a 444-amino acid polypeptide containing three gc1q domains. hcc1qdc6 contains at least five exons and four introns. the mrna transcripts ...201728785225
microencapsulation of essential thyme oil by spray drying and its antimicrobial evaluation against vibrio alginolyticus and vibrio parahaemolyticus.the aims of this research were first, to evaluate the antibacterial potential of commercial thyme essential oil against v. alginolyticus and v. parahaemolyticys and second, using the spray drying technique to produce microcapsules. chemical compounds of thyme oil and microcapsules were identified and quantified being thymol the chemical component present at the highest concentration. oil-in-water (o/w) emulsions were prepared and the microcapsules were obtained with a spray dryer using maltodext ...201728832836
occurrence of vibrio species, beta-lactam resistant vibrio species, and indicator bacteria in ballast and port waters of a tropical harbor.ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. ballast waters from six ships docked in the port of singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation d-2 of the ballast water management convention (bwmc) guidelines. vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant vibr ...201728822933
molecular cloning, characterization and functional analysis of a putative mitogen-activated protein kinase kinase kinase 4 (mekk4) from blood clam tegillarca granosa.the mitogen-activated protein kinase (mapk) cascades stand for one of the most important signaling mechanisms in response to environmental stimuli. in the present study, we cloned and identified for the first time the full-length cdna of mapk kinase kinase 4 (tgmekk4) from blood clam tegillarca granosa using rapid amplification of cdna ends method. the full-length cdna of tgmekk4 was of 1605 bp in length, encoding a polypeptide of 364 amino acids with a predicted molecular mass of 41.22 kda and ...201728476674
environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic vibrio cholerae and vibrio parahaemolyticus strains.with the overuse of antibiotics, many pathogens including vibrio cholerae and vibrio parahaemolyticus have evolved multidrug resistance making treatment more difficult. while understanding the mechanisms that underlie pathogenesis is crucial, knowledge of bacterial interactions of v. cholerae and v. parahaemolyticus could provide insight to their susceptibility outside of the human host. based on previous work showing competition among environmental strains, we predict that marine-derived bacter ...201728857444
effects of desiccation practices of cultured atlantic oysters (crassostrea virginica) on vibrio spp. in portersville bay, alabama, usa.the expansion of off-bottom aquaculture to the gulf of mexico has raised public health concerns for human health officials. high temperatures in the gulf of mexico are associated with high levels of vibrio parahaemolyticus and vibrio vulnificus. routine desiccation practices associated with off-bottom aquaculture expose oysters to ambient air, allowing vibrio spp. to proliferate in the closed oyster. currently, there is limited research on the length of time needed for vibrio spp. levels in desi ...201728696147
a double-quadratic model for predicting vibrio species in water environments of japan.vibrio spp. are natural inhabitants of marine and estuarine environments. vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus are the major infectious agents for humans. their densities are affected by environmental factors such as water temperature and salinity. the detailed contribution of each factor still remains to be elucidated. here we conducted multi-coastal study in a 21-month period to examine relationships between environmental factors and v. cholerae, v. parahaemolyticus ...201728646477
genome analysis of three novel lytic vibrio coralliilyticus phages isolated from seawater, okinawa, japan.three novel vibrio phages were isolated from seawater in okinawa. the vibrio phage ryc infected vibrio coralliilyticus swa 07, while vibrio phages ckb-s1 and ckb-s2 infected the coral pathogen v. coralliilyticus p1 (lmg 23696). the vibrio phages ckb-s1 and ckb-s2 displayed head-tail structures whereas the vibrio phage ryc showed a tailless non-enveloped capsid. all these vibrio phages contained linear and double-stranded dna. the whole genome sequencing revealed that vibrio phage ryc has a large ...201728689690
antagonistic activity of thymus vulgaris extracts against vibrio species isolated from seafoods.this study examined the antibacterial activity of thymus vulgaris on multiple antibiotic resistant (mar) vibrio parahaemolyticus and vibrio fluvialis isolated from shrimps. the ethanol extract of t. vulgaris antibacterial properties was assessed using the agar diffusion method. survival of test organisms in shrimp meat using different concentrations of t. vulgaris was done using standard method. the quantitative and qualitative phytochemical tests of t. vulgaris extract were determined. the etha ...201728416870
environmental determinants of vibrio parahaemolyticus in the chesapeake bay.vibrio parahaemolyticus naturally-occurs in brackish and marine waters and is one of the leading causes of seafood-borne illness. previous work studying the ecology of v. parahaemolyticus is often limited in geographic extent and lacking a full range of environmental measures. this study used a unique, large dataset of surface water samples in the chesapeake bay (n=1,385) collected from 148 monitoring stations from 2007 to 2010. water was analyzed for over 20 environmental parameters with additi ...201728842541
variation of genomic islands and flanking fragments in vibrio parahaemolyticus isolates from environmental and clinical sources in taiwan.vibrio parahaemolyticus is a halophilic foodborne pathogenic bacterium that causes gastroenteritis; it has become an issue of global concern since the emergence and spread of pandemic o3:k6 strains. this study evaluated the role of vibrio pathogenicity island (vpai)-associated fragments in the genetic variation and grouping of this pathogen. distribution of some vpai fragments and flanking fragments (vpai-1, vpai-4, vpai-5, vpai-6 and vpai-7) was determined in a total of 53 v. parahaemolyticus i ...201728841447
inactivation of vibrio parahaemolyticus in shucked raw oyster ( grassostrea gigas) and clam ( venerupis phillippinarum) by using a combination of naclo and gamma irradiation.this study investigated the synergistic effects of sodium hypochlorite (naclo) and gamma irradiation combination against vibrio parahaemolyticus in shucked oysters and clams. v. parahaemolyticus decreased to 1.1-5.6 log10 cfu/g in oysters and 1.1-5.7 log10cfu/g in clams by naclo (20-80 ppm) + γ irradiation (0.1-2.0 kgy) combinations. v. parahaemolyticus was not detected by 60 or 80 ppm naclo + 2.0 kgy. synergistic reduction of >1 log was observed by 60 ppm naclo + 0.3-2 kgy and 80 ppm naclo + 0. ...201728840748
microbiome dynamics in a shrimp grow-out pond with possible outbreak of acute hepatopancreatic necrosis disease.acute hepatopancreatic necrosis disease (ahpnd) (formerly, early mortality syndrome) is a high-mortality-rate shrimp disease prevalent in shrimp farming areas. although ahpnd is known to be caused by pathogenic vibrio parahaemolyticus hosting the plasmid-related pirab(vp) toxin gene, the effects of disturbances in microbiome have not yet been studied. we took 62 samples from a grow-out pond during an ahpnd developing period from days 23 to 37 after stocking white postlarvae shrimp and sequenced ...201728839269
in vitro isothermal nucleic acid amplification assisted surface-enhanced raman spectroscopic (sers) for ultrasensitive detection of vibrio parahaemolyticus.detection of pathogenic microorganisms is of great importance for public health and food safety. traditional protocols can hardly meet the continuously increased demanding in sensitivity and specificity of pathogen detections. in this study, we adopted vibrio parahaemolyticus (v. parahaemolyticus, vp) as the model analyte, and developed an antibody-vp-aptamer hetero-sandwich based surface-enhanced raman scattering (sers) method in conjunction with in vitro isothermal amplification for sensitive ...201728832123
proteomic analysis of vibrio parahaemolyticus under cold stress.vibrio parahaemolyticus is a kind of food-borne pathogenic bacterium, which can seriously infect food, especially seafood causing gastroenteritis and other disease. we studied the global proteome responses of v. parahaemolyticus under cold stress by nano-liquid chromatography-tandem mass spectrometry to improve the present understanding of v. parahaemolyticus proteomics events under cold stress. a total of 1151 proteins were identified and 101 proteins were differentially expressed, of which 69 ...201728831596
characterization, expression, and evolutionary analysis of new tlr3 and tlr5m genes cloned from the spiny eel mastacembelus armatus.toll-like receptors (tlrs) play an important role in innate and adaptive immunity. here, we identify two new tlrs from the spiny eel mastacembelus armatus (tlr3 and membrane tlr5m). both matlr3 and matlr5m were expressed in all tested tissues; expression was highest in liver and spleen, respectively. after infection with vibrio parahaemolyticus, expression of both tlrs fluctuated and differed significantly from controls at several time points. the predicted three-dimensional model of the matlr3 ...201728821419
christmas-tree derived amplification immuno-strategy for sensitive visual detection of vibrio parahaemolyticus based on gold label silver stain technology.a developed christmas-tree derived immunosensor based on a gold label silver stain (glss) technique was fabricated for a highly sensitive analysis of vibrio parahaemolyticu (vp). in this strategy, captured vp antibody (cab) was immobilized on a solid substrate; then, the vps were sequentially tagged with a signal probe by incubating the assay with a detection vp antibody (dab) conjugated gold nanoparticles (aunps)-labeled graphite-like carbon nitride (g-c3n4). finally, the attached signal probe ...201728794324
insight into the origin and evolution of the vibrio parahaemolyticus pandemic strain.a strain of vibrio parahaemolyticus that emerged in 1995 caused the first known pandemic involving this species. this strain comprises clonal autochthonous ocean-dwelling bacteria whose evolution has occurred in the ocean environment. the low sequence diversity in this population enabled the discovery of information on its origin and evolution that has been hidden in bacterial clones that have evolved over a long period. multilocus sequencing and microarray analysis, together with phylogenetic a ...201728790994
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