Publications

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effects of overfeeding on lipid and carbohydrate metabolism in lean young adults. 20165047918
studies on sea urchin oocytes. ii. synthesis of rna during oogenesis. 20165025407
[clinical evaluation of chlorimipramine in depressive states. preliminary report]. 20165091034
editorial: the delaney amendment. 20164803327
self-evaluation by first-year medical students in a clinical science programme. 20164771337
the pragmatic utilization of the p-k phenomenon. 20165039530
[i. from the diary of a young physician of the mission of brazil to france in the 1st world war]. 20164916215
regional morbidity. 20164772208
ultrasonic diagnosis of abdominal aortic aneurysms. 20164846789
[differential diagnosis of "acute appendicitis" (torsion of an epiploic appendix)]. 20165054687
minimally distinct border as a preferred psychophysical criterion in visual heterochromatic photometry. 20165087389
contact dermatitis from cellulose ester plastics. 20165030237
[therapeutic application of the preparation d 1 s in cardio-vascular neurosis]. 20165018516
can we avoid a 100 billion dollars misunderstanding? 20164992557
guidelines to research. 20164924037
resonance energy transfer in pepsin conjugates. 20164897805
bibliography on medical education. citations obtained through the national library of medicine's medlars program. 20164866023
hypersomatotropism and acanthosis nigricans in two brothers. 20164843205
immigration composition and population policy. 20164841571
pathologic quiz case 1. 20164809945
bobaths' facilitation training and seguin's physiological method related to escape-avoidance conditioning. 20164808072
metastatic urethral obstruction. 20164751838
maytansine, a novel antileukemic ansa macrolide from maytenus ovatus. 20165062169
metabolism of the heart in health and disease. ii. 20164882561
portable transistor instrument for recording the galvanic skin response. 20164817134
parkinsonism, l-dopa, and intelligence. 20164754685
[examinations on the influence of arterial blood pressure on the course of glaucoma simplex]. 20165095602
[successfully treated case of traumatic ventricular septal perforation caused by stabbing]. 20165090175
contrast laryngography in diagnosis. 20165067765
[late results of surgery on the thyroid gland]. 20165068155
simultaneous dispersion and decay of radioactive wastes. 20165014874
are you receiving me? 20165014404
[chromatoclastic and mitoclastic activity of some fractions of tars of cigarette smoke]. 20164987574
rejection of the generic name dendrocystis rees, 1962, and its replacement by arborocystis nov.nom. protozoa: gregarinomorpha. 20164960245
[transduodenal papillotomy and sphincterotomy in biliary surgery: general review of 132 cases]. 20164913238
[death of dr. guillermo mann fischer]. 20164877508
subacute sclerosing encephalitis. 20164862504
[letter: homologous insemination of poole sperma]. 20164849080
[our equipment for cortical audiometry]. 20164837373
letter: how to stamp out creativity. 20164820304
environmental lead pollution. 20164821235
letter: on lid loading in the management of lagophthalmos. 20164843124
letter: beyond cost estimating, another ball game. 20164820302
proceedings: factors influencing the outcome of chemotherapy of brain tumors. 20164764938
some genetic traits in solomon island populations. 3. relative toe length. 20164750669
[aging of the patients with old cranial injuries. (catamnestic study of 88 patients hospitalized in psychiatric environment)]. 20164725280
we broke the communication barrier. 20165076704
pseudomyopia as a presenting sign in ocular myasthenia gravis. 20164706420
preparation, characterization and antibacterial activity of chitosan and phosphorylated chitosan from cuttlebone of sepia kobiensis (hoyle, 1885).chitosan is a commercially available derivative of chitin that has been extensively studied for its antimicrobial properties. in order to improve the water solubility and its biological activity, the chemical modification or derivatisation is attempted. in the present investigation, the chitosan prepared from the cuttlebone of sepia kobiensis was being chemically modified by reacting it with orthophosphoric acid so as to obtain phosphorylated chitosan. then the chitosan and phosphorylated chitos ...201628507912
centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection.we present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (lamp) and colorimetric detection using eriochrome black t (ebt). five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. the sequential loading and aliquoting of the lamp cocktail, the primer mixtures, and the dna sample solutions were accomplishe ...201626322592
zebrafish as a useful model for zoonotic vibrio parahaemolyticus pathogenicity in fish and human.vibrio parahaemolyticus is an important aquatic zoonotic pathogen worldwide that causes vibriosis in many marine fish, and sepsis, gastroenteritis and wound infection in humans. however, the pathogenesis of different sources of v. parahaemolyticus is not fully understood. here, we examined the pathogenicity and histopathology of fish (v. parahaemolyticus 1.2164) and human (v. parahaemolyticus 17) strains in a zebrafish (danio rerio). we found that different infection routes resulted in different ...201626519599
prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from oysters in korea.vibrio parahaemolyticus is the most prevalent gastroenteritis-causing pathogen in korea and in some other asian countries. it is frequently found in oysters and other seafood. this study monitored changes in the prevalence of v. parahaemolyticus and environmental parameters in oyster aquaculture environments in korea. from june to october 2014, we tested oysters (crassostrea gigas) from shellfish-harvesting areas off the west coast of korea. these 71 isolates were the sum of 16 (22.5%), 19 (26.8 ...201626511256
detection, identification, and prevalence of pathogenic vibrio parahaemolyticus in fish and coastal environment in jordan.vibrio parahaemolyticus is widely distributed in the marine environments and considered the leading cause of human gastroenteritis in asian countries. a total of 150 marketed fish and 50 water and sediment samples from the gulf of aqaba were examined for the prevalence of pathogenic strains of v. parahaemolyticus. a total of 132 typical isolates obtained from the primary selective medium (thiosulfate-citrate bile salt sucrose agar) and showed positive biochemical properties were subjected to con ...201626554333
epidemiological investigation of a foodborne outbreak in spain associated with u.s. west coast genotypes of vibrio parahaemolyticus.we describe an outbreak of seafood-associated vibrio parahaemolyticus in galicia, spain in on 18th of august 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. the food items identified through the questionnaires as the most probable source of the infections was shrimp. this product was unique in showing a statistically significant and ...201626848427
serology, virulence, antimicrobial susceptibility and molecular characteristics of clinical vibrio parahaemolyticus strains circulating in southeastern china from 2009 to 2013.vibrio parahaemolyticus is a leading cause of food-borne diarrhoea in coastal countries. although v. parahaemolyticus cases have been reported since 1950, they have been poorly documented. from july 2009 to june 2013, we collected 6951 faecal specimens for pathogen detection; v. parahaemolyticus strains were isolated from 563 specimens (8.1%). we then analysed the characteristics of the 501 v. parahaemolyticus strains that were isolated as the sole pathogen. twenty-one serotypes were identified ...201626597222
a label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of vibrio parahaemolyticus in seawater and seafood.a label-free electrochemiluminescence (ecl) immunosensor for ultrasensitive and rapid detection of marine pathogenic bacterium vibrio parahaemolyticus (vp) in seawater and seafood was developed based on multi-functionalized graphene oxide, which was prepared with n-(4-aminobutyl)-n-ethylisoluminol (abei) and vp antibody (anti-vp) simultaneously immobilized on the surface of magnetic graphene oxide (nanofe3o4@go). abei and anti-vp acted as the electrochemiluminophore and the capture device for vp ...201626592599
genetic diversity of vibrio parahaemolyticus strains isolated from farmed pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in thailand.acute hepatopancreatic necrosis disease (ahpnd) is an emerging shrimp disease that causes massive die-offs in farmed shrimps. recent outbreaks of ahpnd in asia have been causing great losses for shrimp culture and have become a serious socioeconomic problem. the causative agent of ahpnd is vibrio parahaemolyticus, which is typically known to cause food-borne gastroenteritis in humans. however, there have been few reports of the epidemiology of v. parahaemolyticus ahpnd strains, and the genetic r ...201626590959
prevalence and factors associated with different pathogens of acute diarrhea in adults in beijing, china.only a small proportion of patients with diarrhea are diagnosed with laboratory tests in china, and most are diagnosed based on clinical symptoms. therefore, understanding the prevalence of different diarrheal pathogens and their specific symptoms is important.201627886032
differential localization of chemotactic signaling arrays during the lifecycle of vibrio parahaemolyticus.when encountering new environments or changes to their external milieu, bacteria use elaborate mechanisms to respond accordingly. here, we describe how vibrio parahaemolyticus coordinates two such mechanisms - differentiation and chemotaxis. v. parahaemolyticus differentiates between two distinct cell types: short rod-shaped swimmer cells and highly elongated swarmer cells. we show that the intracellular organization of chemotactic signaling arrays changes according to the differentiation state. ...201627853457
draft genome sequences of one marine and one clinical vibrio parahaemolyticus strain, both isolated in sweden.vibrio parahaemolyticus is the leading bacterial pathogen associated with seafood consumption. here, we report the draft genome sequences of one marine and one clinical strain, both isolated in sweden. these sequences will inform future comparative analysis of v. parahaemolyticus in northern europe.201627789643
sensor histidine kinase is a β-lactam receptor and induces resistance to β-lactam antibiotics.β-lactams disrupt bacterial cell wall synthesis, and these agents are the most widely used antibiotics. one of the principle mechanisms by which bacteria resist the action of β-lactams is by producing β-lactamases, enzymes that degrade β-lactams. in gram-negative bacteria, production of β-lactamases is often induced in response to the antibiotic-associated damage to the cell wall. here, we have identified a previously unidentified mechanism that governs β-lactamase production. in the gram-negati ...201626831117
pathogenicity of vibrio parahaemolyticus in different food matrices.the pathogenicity and virulence factors of vibrio parahaemolyticus in four food matrices--shrimp, freshwater fish, pork, and egg-fried rice--were compared by measuring the thermostable direct hemolysin activity and total hemolytic titer. significantly high thermostable direct hemolysin and also hemolytic titers (p < 0.05) were produced by v. parahaemolyticus in egg-fried rice > shrimp > freshwater fish > pork. filtrates of v. parahaemolyticus in shrimp given intraperitoneally induced marked live ...201626818990
biocompatibility and antibacterial activity of the adathoda vasica linn extract mediated silver nanoparticles.the aim of this study is to investigate the biocompatibility and anti-vibrio efficacy of green synthesized silver nanoparticles (agnps) using an aqueous leaf extract of adathoda vasica (a. vasica). the green synthesized silver nanoparticles were characterized by uv-vis, fourier transform infrared (ftir) spectroscopy, x-ray diffraction (xrd), transmission electron microscopy (tem), scanning electron microscopy (sem) and energy dispersive x-ray analysis (edx). a. vasica agnps showed significant an ...201626802519
rapid and sensitive detection of vibrio parahaemolyticus and vibrio vulnificus by multiple endonuclease restriction real-time loop-mediated isothermal amplification technique.vibrio parahaemolyticus and vibrio vulnificus are two marine seafood-borne pathogens causing severe illnesses in humans and aquatic animals. in this study, a recently developed novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (mert-lamp) were successfully developed and evaluated for simultaneous detection of v. parahaemolyticus and v. vulnificus strains in only a single reaction. two mert-lamp primer sets were designed to specifically target tox ...201626797596
serogroup, virulence, and molecular traits of vibrio parahaemolyticus isolated from clinical and cockle sources in northeastern thailand.vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. isolates of v. parahaemolyticus from clinical samples (n=74) and cockles (anadara granosa) (n=74) in thailand were analyzed by serotyping, determination of virulence and related marker genes present, response to antimicrobial agents, and genetic relatedness. serological analysis revealed 31 different serotypes, 10 of which occurred among both clinical and cockle samples. the clinical isolates commonly included th ...201626773828
identification and pathogenicity of vibrio parahaemolyticus isolates and immune responses of penaeus (litopenaeus) vannamei (boone).five different vibrio parahaemolyticus strains (sh8, sh108, sh58, ah5 and gd10) isolated from the hepatopancreas of moribund shrimp in farms of mainland china were identified and capable of inducing massive mortality of penaeus (litopenaeus) vannamei. the immersion challenge results with five isolates indicated variance of virulence, while only gd10 caused massive sloughing of tubule epithelial cells which was recognized as the most significant symptom of ahpnd. differences in immune responses w ...201626763100
identification and characterization of mkk7 as an upstream activator of jnk in litopenaeus vannamei.mitogen-activated protein kinase kinase 7 (mkk7) is a key signal transduction regulator in c-jun n-terminal kinase (jnk) signaling pathway, which is involved in a wide range of physiological and pathological processes. in this study, we described the molecular cloning of a new member of mkk7 group from litopenaeus vannamei named as lvmkk7. the full-length cdna of lvmkk7 was 3093 bp in length, with an open reading frame (orf) of 1440bp encoding a putative protein of 479 amino acids. lvmkk7 contai ...201626707780
two distinct interferon-γ genes in tetraodon nigroviridis: functional analysis during vibrio parahaemolyticus infection.interferon gamma (ifnγ) is a th1 cytokine that plays a very important role in almost all phases of immune and inflammatory responses. in this study, we explored the functions of ifnγ1 and ifnγ2 of tetraodon nigroviridis. treating t. nigroviridis spleen and head kidney cells in vitro with recombinant t. nigroviridis ifnγ1 protein (rtn ifnγ1) or recombinant t. nigroviridis ifnγ2 protein (rtn ifnγ2) enhanced their nitric oxide responses. both rtn ifnγ1 and rtn ifnγ2 also induced the expression of i ...201626701668
inactivation of vibrio parahaemolyticus unattached and attached to a solid surface in ph-controlled sodium hypochlorite solutions.the inactivation of vibrio parahaemolyticus cells that were unattached or attached to a polyethylene terephthalate (pet) disc in ph-controlled sodium hypochlorite (naocl) solutions was studied under turbulent conditions. no significant desorption of attached cells occurred at the free available chlorine (fac) concentrations from 0.1 to 1.0 mg/l. the number of viable cells was estimated by microbial calorimetry. the logarithmic relative reduction of viable cells was proportional to the product of ...201628003634
actin cytoskeleton-modulating t3ss2 effectors and their contribution to the vibrio parahaemolyticus-induced diarrhea.to understand how bacterial pathogens cause diseases is the most important step in order to prevent the infection and develop an effective treatment. however, the past proceeding studies make us aware of quite-complicated interactions between the host and pathogenic bacteria. vibrio parahaemolyticus, a food-born pathogen that is a subject of our study, causes inflammatory diarrhea in human upon ingestion of contaminated raw or undercooked seafood. many virulence factors has been proposed since i ...201627980291
first multi-year retrospective study on vibrio parhaemolyticus and vibrio vulnificus prevalence in ruditapes philippinarum harvested in sacca di goro, italy.the present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of vibrio spp., and the prevalence of v. parahaemolyticus and v. vulnificus in the manila clam r. philippinarum harvested in sacca di goro, emilia-romagna region, northern italy. on the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have ...201628058248
development of a more sensitive and specific chromogenic agar medium for the detection of vibrio parahaemolyticus and other vibrio species.foodborne infections in the us caused by vibrio species have shown an upward trend. in the genus vibrio, v. parahaemolyticus is responsible for the majority of vibrio-associated infections. thus, accurate differentiation among vibrio spp. and detection of v. parahaemolyticus is critically important to ensure the safety of our food supply. although molecular techniques are increasingly common, culture-depending methods are still routinely done and they are considered standard methods in certain c ...201627911360
evaluation of medicinal plants and colloidal silver efficiency against vibrio parahaemolyticus infection in litopenaeus vannamei cultured at low salinity.in shrimp aquaculture, reduction in the use of synthetic antibiotics is a priority due to the high incidence of resistant bacteria (vibrio) in the white shrimp litopenaeus vannamei. an increasing number of studies show bactericidal activity of natural treatments in aquaculture. the effectiveness of neem (azadirachta indica) and oregano (lippia berlandieri) aqueous extracts and colloidal silver against v. parahaemolyticus were evaluated in low salinity shrimp culture. results show that aqueous ex ...201627901504
pi3k-akt signaling pathway is involved in hypoxia/thermal-induced immunosuppression of small abalone haliotis diversicolor.the pi3k-akt signal pathway has been found to be involved in many important physiological and pathological processes of the innate immune system of vertebrates and invertebrates. in this study, the akt (hdakt) and pi3k (hdpi3k) gene of small abalone haliotis diversicolor were cloned and characterized for the important status of pi3k and akt protein in pi3k-akt signaling pathway. the full length cdnas of hdakt and hdpi3k are 2126 bp and 6052 bp respectively, encoding proteins of 479 amino acids a ...201627825946
a c-type lectin (mrlec) with high expression in intestine is involved in innate immune response of macrobrachium rosenbergii.c-type lectins (ctls) are pattern-recognition proteins that play an important role in innate immunity of vertebrates and invertebrates. in this study, a lectin cdna named mrlec was cloned and characterized from giant freshwater prawns (macrobrachiun rosenbergii). the full-length cdna of mrlec was 1431 bp, which contained an open reading frame of 1041 bp that encoded a protein with 346 amino acids. mrlec was found to contain a typical signal peptide of 18 amino acids and a single carbohydrate-rec ...201627818342
in vivo activity and the transcriptional regulatory mechanism of the antimicrobial peptide sphyastatin in scylla paramamosain.a new gene homologous to the reported antimicrobial peptide (amp) hyastatin from hyas araneus was screened in the ssh library constructed from the hemocytes of scylla paramamosain, and named sphyastatin. in vivo study showed that sphyastatin was predominantly expressed in hemocytes of s. paramamosain. with the challenge of either vibrio parahaemolyticus or lipopolysaccharide (lps), sphyastatin showed a positive response, meaning that it was probably involved in the immune reaction against bacter ...201627751917
virulence of acute hepatopancreatic necrosis disease pirab-like relies on secreted proteins not on gene copy number.to investigate the virulence of the vp_pirab-like genes in vibrio parahaemolyticus- acute hepatopancreatic necrosis disease (ahpnd)-causing strain and the factors that are associated with the virulence level.201627522063
regulation of the expression of iron-acquisition system genes in pathogenic vibrio species.the genus vibrio includes >70 species, of which roughly a dozen cause vibriosis such as gastroenteritis, wound infections, and septicemia. most bacteria, including vibrio species, require iron for survival and growth. however, the bioavailability of iron is extremely low because it is usually present as an insoluble ferric complex in an aerobic environment or is bound to iron-binding proteins in mammalian hosts. therefore many bacteria have developed iron acquisition systems, including biosynthe ...201627803484
pharmacokinetic indices for cefovecin after single-dose administration to adult sea otters (enhydra lutris).seven sea otters received a single subcutaneous dose of cefovecin at 8 mg/kg body weight. plasma samples were collected at predetermined time points and assayed for total cefovecin concentrations using ultra-performance liquid chromatography and tandem mass spectrometry. the mean (±sd) noncompartmental pharmacokinetic indices were as follows: cmax (obs) 70.6 ± 14.6 μg/ml, tmax (obs) 2.9 ± 1.5 h, elimination rate constant (kel ) 0.017 ± 0.002/h, elimination half-life (t1/2kel) 41.6 ± 4.7 h, area ...201627212054
development of a highly sensitive lateral immunochromatographic assay for rapid detection of vibrio parahaemolyticus.vibrio parahaemolyticus is widely present in brackish water all over the world, causing infections in certain aquatic animals. it is also a foodborne pathogen that causes diarrhea in humans. the aim of this study is to develop an immunochromatographic lateral flow assay (lfa) for rapid detection of v. parahaemolyticus in both aquatic products and human feces of diarrheal patients. two monoclonal antibody (mab) pairs, ga1a-ic9 and ic9-kb4c, were developed and proven to be highly specific and sens ...201627751742
c-type lectin (mrctl) from the giant freshwater prawn macrobrachium rosenbergii participates in innate immunity.c-type lectins (ctls) play important roles in the innate immunity of invertebrates. in this study, a novel ctl with a single carbohydrate recognition domain (crd) containing an epn (glu-pro-asn) motif was identified from the giant freshwater prawn macrobrachium rosenbergii. this ctl was designated as mrctl. the cdna of mrctl is 1788 bp with a 657 bp open reading frame that encodes a protein of 218 amino acids. the cdna and genome sequences of mrctl show a polymorphism that leads to mrctl isoform ...201627620819
population analysis of clinical and environmental vibrio parahaemolyticus isolated from eastern provinces in china by removing the recombinant snps in the mlst loci.vibrio parahaemolyticus is a common seafood-borne pathogenic bacterium which causes gastroenteritis in humans. continuous surveillance on the molecular characters of the clinical and environmental v. parahaemolyticus strains needs to be conducted for the epidemiological and genetic purposes. to generate a picture of the population distribution of v. parahaemolyticus in eastern china isolated from clinical cases of gastroenteritis and environmental samples, we investigated the genetic and evoluti ...201627608607
characteristics of vibrio parahaemolyticus isolates obtained from crayfish (procambarus clarkii) in freshwater.vibrio parahaemolyticus usually occurs in coastal areas and is generally recognized as a marine bacterium. it has become the leading cause of gastroenteritis worldwide. in the present study, 96 v. parahaemolyticus isolates were obtained from freshwater crayfish (procambarus clarkii) and classified by multilocus sequence typing. fifty-three sequence types (sts) were identified among the 96 isolates analyzed, 38 of which were novel sts. these isolates fell into six groups and 42 singletons, sugges ...201627620824
vibrio parahaemolyticus and vibrio vulnificus in south america: water, seafood and human infections.the bacterial species, vibrio parahaemolyticus and vibrio vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. they are concentrated by filter-feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. vibrio parahaemolyticus can cause abdominal cramping, nausea, diarrhoea, vomiting, chills and fever. vibrio vulnificus can ...201627459915
genome sequence of vibrio parahaemolyticus vp103 strain isolated from shrimp in malaysia. 201627708636
expression patterns of two heat-shock cognate 70 genes during immune responses and larval development of the chinese mitten crab eriocheir sinensis.two heat-shock protein (hsp) 70 family transcripts, heat-shock protein 70 cognate 5 and heat-shock protein 70 cognate 3 (designated as eshsc70-5 and eshsc70-3, respectively), were isolated from the chinese mitten crab eriocheir sinensis and their expression profiles were evaluated for their responsiveness to larval development and immune challenge in adult crabs. the hsps exhibited 45-89% identity with other heat-shock proteins, and they shared similar structural features. eshsc70 mrna expressio ...201627706716
genome sequence of vibrio parahaemolyticus vp152 strain isolated from penaeus indicus in malaysia. 201627656174
genetic characterization of a blaveb-2-carrying plasmid in vibrio parahaemolyticus.this report describes the first detection of a blaveb-2 gene in a vibrio parahaemolyticus strain isolated from a shrimp sample. the blaveb-2 gene was carried on a novel inc-type plasmid that was likely to have originated from aquatic organisms, as indicated by a comparison with other known genetic elements in the genbank database. however, the plasmid contains resistance elements usually harbored by members of the family enterobacteriaceae, suggesting that gene transfer events occurred and contr ...201627645248
trh (tdh-/trh+) gene analysis of clinical, environmental and food isolates of vibrio parahaemolyticus as a tool for investigating pathogenicity.sequencing analysis of the trh gene encoding the tdh-related haemolysin of tdh-/trh+ vibrio parahaemolyticus isolated in italy between 2002 and 2011 from clinical, environmental, and food samples revealed the presence of the trh2 variant in all isolates. the trh2 of the clinical isolate was 100% identical to other clinical tdh-/trh2 v. parahaemolyticus from europe. nucleotide and amino acid differences in the trh2 sequences of clinical isolates from italy and other countries allowed a differenti ...201626990408
variability in biofilm formation correlates with hydrophobicity and quorum sensing among vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in biofilm formation.vibrio parahaemolyticus is one of the leading foodborne pathogens causing seafood contamination. here, 22 v. parahaemolyticus strains were analyzed for biofilm formation to determine whether there is a correlation between biofilm formation and quorum sensing (qs), swimming motility, or hydrophobicity. the results indicate that the biofilm formation ability of v. parahaemolyticus is positively correlated with cell surface hydrophobicity, autoinducer (ai-2) production, and protease activity. field ...201626980068
genome characterization of a novel vibriophage vpkk5 (siphoviridae) specific to fish pathogenic strain of vibrio parahaemolyticus.vibrio parahaemolyticus has long been known pathogenic to shrimp but only recently it is also reported pathogenic to tropical cultured marine finfish. traditionally, bacterial diseases in aquaculture are often treated using synthetic antibiotics but concern due to side effects of these chemicals is elevating hence, new control strategies which are both environmental and consumer friendly, are urgently needed. one promising control strategy is the bacteriophage therapy. in this study, we report t ...201626960780
prevalence, characterization, and antibiotic susceptibility of vibrio parahaemolyticus isolated from retail aquatic products in north china.vibrio parahaemolyticus is a major foodborne pathogen, particularly in asian countries. increased occurrence of outbreaks of v. parahaemolyticus gastroenteritis in china indicates the need to evaluation of the prevalence of this pathogenic species. v. parahaemolyticus distribution in shellfish from the eastern coast of china has been reported previously. however, to date, the prevalence of v. parahaemolyticus in retail aquatic products in north china has not been determined. to investigate the p ...201626955871
essential oils of nigella sativa protects artemia from the pathogenic effect of vibrio parahaemolyticus dahv2.the anti-vibrio activity of essential oils (eos) of nine medicinal plants was tested against 28 vibrio spp. isolated from diseased fenneropenaeus indicus. eo of nigella sativa exhibited anti-vibrio activity against all vibrio spp. and greater inhibition was noted for the isolate v2 which was identified as vibrio parahaemolyticus dahv2. further, eo of n. sativa effectively inhibited v. parahaemolyticus dahv2 with an inhibition zone of 23.9mm at 101.2μgml(-1). moreover, eo of n. sativa revealed an ...201626945773
incidence rates and clinical symptoms of salmonella, vibrio parahaemolyticus, and shigella infections in china, 1998-2013.the etiological and clinical characteristics of patients with infectious diarrhea have changed during the last decade in shanghai.201626927452
single crd containing lectin from macrobrachium rosenbergii (mrlec) participates in innate immunity against pathogen infections.as a type of pattern-recognition proteins, lectins perform important functions in the innate immunity of crustaceans, including prawns. although several reports showed that c-type lectin domain family (clec) importantly functions in host-pathogen interactions, limited research has focused on clec in macrobrachium rosenbergii. in the present study, a new single crd containing clec (designated as mrlec) was reported in freshwater prawns, m. rosenbergii. the full-length cdna of mrlec consisted of 1 ...201626923244
editorial: ecology, virulence, and detection of pathogenic and pandemic vibrio parahaemolyticus. 201626909072
protective roles of katg-homologous genes against extrinsic peroxides in vibrio parahaemolyticus.the marine foodborne enteropathogen, vibrio parahaemolyticus, has four putative catalase genes. function of the katg-homologous genes, katg1(vpa0768) and katg2(vpa0453), was examined using gene deletion mutants, and compared with those of the kate-homologous genes, kate1(vpa1418) and kate2(vpa0305). bacterial growth of δkatg1 was significantly delayed in the presence of 200-300 μm h2o2, and such inhibition was enhanced when incubation temperature was lowered from 37°c to 22°c. in the stationary ...201626892020
variations of immune parameters in the lined seahorse hippocampus erectus after infection with enteritis pathogen of vibrio parahaemolyticus.enteritis has been increasingly recognized as one of the major obstacles for the lined seahorse hippocampus erectus mass culture success. in the present study, the intestinal bacteria strains of the lined seahorses h. erectus suffered from enteritis were isolated, then their pathogenicities were confirmed by artificial infection, and one pathogenic bacteria strain named ds3 was obtained. the median lethal dose (ld50) of strain ds3 for 10 days was determined. the seahorses with different infectio ...201626851568
responses of three very large inducible gtpases to bacterial and white spot syndrome virus challenges in the giant fresh water prawn macrobrachium rosenbergii.interferons (ifns) are cytokines secreted by cells in response to invasion by pathogens, such as viruses, bacteria, parasites, or tumor cells. very large inducible gtpases (vlig) are the latest ifn-inducible gtpase family to be discovered and are the largest known gtpases of any species. however, vlig proteins from invertebrates have yet to be characterized. in this study, three forms of vligs designated as mrvlig1, mrvlig2, and mrvlig3 were cloned from the giant fresh water prawn macrobrachium ...201626850335
passive immune-protection of litopenaeus vannamei against vibrio harveyi and vibrio parahaemolyticus infections with anti-vibrio egg yolk (igy)-encapsulated feed.vibrio spp. are major causes of mortality in white shrimp (litopenaeus vannamei) which is lacking adaptive immunity. passive immunization with a specific egg yolk antibody (igy) is a potential method for the protection of shrimp against vibriosis. in this study, immune effects of the specific egg yolk powders (igy) against both v. harveyi and v. parahaemolyticus on white shrimp were evaluated. the egg yolk powders against v. harveyi and v. parahaemolyticus for passive immunization of white shrim ...201627196895
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