Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| dual infection by chikungunya virus and other imported infectious agent in a traveller returning from india. | chikungunya virus infection is a vector-borne self-limiting disease. recent outbreaks in the indian ocean islands have drawn attention to the condition. nevertheless, only a few reports of co-infection with other communicable agents have been reported. the case described now is of a traveller returning from india with concomitant documented chikungunya virus infection associated with systemic amoebiasis. this report highlights the multifaceted pathology that can be encountered with tropical infe ... | 2008 | 18486073 |
| design, synthesis and in vitro antiprotozoal activity of benzimidazole-pentamidine hybrids. | a series of ten novel hybrids from benzimidazole and pentamidine were prepared using a short synthetic route. each compound was tested in vitro against the protozoa trichomonas vaginalis, giardia lamblia, entamoeba histolytica, leishmania mexicana, and plasmodium berghei, in comparison with pentamidine and metronidazole. some analogues showed high bioactivity in the low micromolar range (ic(50)<1 microm) against the first four protozoa, which make them significantly more potent than either stand ... | 2008 | 18486471 |
| acanthamoeba castellanii: identification and distribution of actin cytoskeleton. | the presence of the cytoskeleton of acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. this structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic structures. in addition, the comparison between total actin content in whole extracts among different amoebae was made. the molecular weight of actin in a. castellanii was 44 kda, and 45 ... | 2008 | 18486940 |
| clinical and serological evidence of toxocara infection in school children from morrope district, lambayeque, peru. | the aim of this study was to estimate the frequency of human toxocariosis in a child population from morrope district, lambayeque, peru. from october to december 2005, 182 school children (96 male and 86 female) were studied. blood samples were collected for toxocara elisa-igg test and hematological examination. additionally, stool samples were collected for coproparasitological examination to check cross reactions. we found frequency of positives in 32.4% (59/182) with a significant higher prop ... | 2008 | 18488089 |
| molecular modeling and docking analysis of entamoeba histolytica glyceraldehyde-3 phosphate dehydrogenase, a potential target enzyme for anti-protozoal drug development. | the enzymes of the glycolytic pathway constitute an excellent target for the design of new anti-protozoal agents; glyceraldehyde-3 phosphate dehydrogenase catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (gap) into d-glycerate 1,3-bisphosphate (1,3-dipg) in the presence of nad+ and inorganic phosphate (p(i)). the prediction of three-dimensional structure of entamoeba histolytica and the docking of the coumarins were performed using the crystal structure of glyce ... | 2008 | 18489439 |
| seroprevalence of viral hepatitis and sexually transmitted disease among adults with recently diagnosed hiv infection in southern taiwan, 2000-2005: upsurge in hepatitis c virus infections among injection drug users. | the purpose of this study was to compare the seroprevalence of viral hepatitis and sexually transmitted disease (std) co-infections among three populations at risk recently diagnosed with hiv infection. | 2008 | 18492625 |
| epigenetic transcriptional gene silencing in entamoeba histolytica. | the human intestinal pathogen entamoeba histolytica has a number of virulence factors which can cause damage to the host. transcriptional silencing of the gene coding for one of its major toxic molecules, the amoebapore (ehap-a), occurred following the transfection of amoebic trophozoites with a plasmid containing the 5' promoter region of ehap-a as well as a truncated segment of a neighboring, upstream sine1 element that is transcribed from the opposite strand. silencing was dependent on the pr ... | 2008 | 18493998 |
| protein ser/thr phosphatases of parasitic protozoa. | protein phosphorylation is an important mechanism implicated in physiology of any organism, including parasitic protozoa. enzymes that control protein phosphorylation (kinases and phosphatases) are considered promising targets for drug development. this review attempts to provide the first account of the current understanding of the structure, regulation and biological functions of protein ser/thr phosphatases in unicellular parasites. we have examined the complements of phosphatases ("phosphato ... | 2008 | 18619495 |
| the risk of pathogenic intestinal parasite infections in kisii municipality, kenya. | intestinal parasitic infections are among the most common infections worldwide. various epidemiological studies indicate that the prevalence of intestinal parasites is high especially in developing countries, although in many of these, the environmental risk factors have not been clearly elucidated. the objective of this study was to determine the risk of pathogenic intestinal parasites infections in kisii municipality. | 2008 | 18620608 |
| amoebiasis among patrons visiting gay saunas in taiwan. | this study aimed to assess the prevalence of amoebiasis among patrons visiting gay saunas in taiwan. | 2008 | 18624723 |
| ehhmbp45 is a novel hemoglobin-binding protein identified in entamoeba histolytica. | hemoglobin-binding proteins are necessary for pathogens to obtain iron from hb. entamoeba histolytica can grow using hb as source of iron, but the underlying mechanism has not previously been established. in this work, we identified a 45 kda hb-binding protein of e. histolytica, which we named ehhmbp45. in silico analysis showed that ehhmbp45 contains the conserved domains needed for hb-binding, while overlay assays demonstrated that ehhmbp45 is able to bind hb. in addition, we found that ehhmbp ... | 2008 | 18625230 |
| amebic liver abscess in a patient with polycystic disease. | 2008 | 18626440 | |
| extracellular alix regulates integrin-mediated cell adhesions and extracellular matrix assembly. | alix (alg-2-interacting protein x), a cytoplasmic adaptor protein involved in endosomal sorting and actin cytoskeleton assembly, is required for the maintenance of fibroblast morphology. as alix has sequence similarity to adhesin in entamoeba histolytica, and we observed that alix is secreted, we determined whether extracellular alix affects fibroblast morphology. here, we demonstrate that secreted alix is deposited on the substratum of non-immortalized wi38 fibroblasts. antibody binding to extr ... | 2008 | 18636094 |
| enteric parasitic infections in hiv/aids patients before and after the highly active antiretroviral therapy. | enteroparasites are related to gastrointestinal alterations among patients with hiv/aids, some causing severe manifestations in the period before the institution of the highly active antiretroviral therapy (haart). the prevalence of enteroparasitoses in patients with hiv/aids seen at two hospitals in ceará , brazil, was compared in the pre-haart (group 1; n = 482) and haart (group 2; n = 100) eras. fecal parasitologic examinations (fpe) were performed using the direct, lutz, baermann-moraes and ... | 2008 | 18641847 |
| primary cutaneous amebiasis with a fatal outcome. | we report a fatal case of disseminated amebiasis in a young african woman, which initially presented with an ulcerated cutaneous lesion on the left flank. the causative organism was confirmed by examination of a wet drop preparation from the ulcer discharge and by skin biopsy. the patient was not immunosuppressed and was treated unsuccessfully with metronidazole. postmortem examination revealed the presence of intestinal amebiasis complicated by a liver abscess. | 2008 | 18645316 |
| [the prevalence of intestinal parasites according to the distribution of the patients' gender and parasite species for five years at the osmangazi university medical faculty]. | in this study, patients (outpatients and inpatients) with various gastrointestinal system complaints presenting at various clinics of the eskisehir osmangazi university medical faculty, from february 2003-december 2007 were investigated for the presence of intestinal parasites. the prevalence of intestinal parasites was evaluated according to parasite species, gender of the patients and the years, in which cases were seen. a total of 34,733 stool samples were prepared by formal-ethyl acetate con ... | 2008 | 18645941 |
| the interplay between entamoeba and enteropathogenic bacteria modulates epithelial cell damage. | mixed intestinal infections with entamoeba histolytica, entamoeba dispar and bacteria with exacerbated manifestations of disease are common in regions where amoebiasis is endemic. however, amoeba-bacteria interactions remain largely unexamined. | 2008 | 18648517 |
| characterization of an entamoeba histolytica high-mobility-group box protein induced during intestinal infection. | the unicellular eukaryote entamoeba histolytica is a human parasite that causes amebic dysentery and liver abscess. a genome-wide analysis of gene expression modulated by intestinal colonization and invasion identified an upregulated transcript that encoded a putative high-mobility-group box (hmgb) protein, ehhmgb1. we tested if ehhmgb1 encoded a functional hmgb protein and determined its role in control of parasite gene expression. recombinant ehhmgb1 was able to bend dna in vitro, a characteri ... | 2008 | 18658254 |
| experimental validation of metabolic pathway modeling. | in the search for new drug targets in the human parasite entamoeba histolytica, metabolic control analysis was applied to determine, experimentally, flux control distribution of amebal glycolysis. the first (hexokinase, hexose-6-phosphate isomerase, pyrophosphate-dependent phosphofructokinase (pp(i)-pfk), aldolase and triose-phosphate isomerase) and final (3-phosphoglycerate mutase, enolase and pyruvate phosphate dikinase) glycolytic segments were reconstituted in vitro with recombinant enzymes ... | 2008 | 18510554 |
| intestinal invasion by entamoeba histolytica. | 2008 | 18512355 | |
| in silico analysis of est and genomic sequences allowed the prediction of cis-regulatory elements for entamoeba histolytica mrna polyadenylation. | in most eukaryotic cells, the poly(a) tail at the 3'-end of messenger rna (mrna) is essential for nuclear export, translatability, stability and transcription termination. poly(a) tail formation involves multi-protein complexes that interact with specific sequences in 3'-untranslated region (3'-utr) of precursor mrna (pre-mrna). here we have performed a computational analysis of a large est and genomic sequences collection from entamoeba histolytica, the protozoan parasite responsible for human ... | 2008 | 18514032 |
| viral, bacterial and parasitic etiology of pediatric diarrhea in gaza, palestine. | to determine the etiology of acute diarrhea in palestinian children under 5 years of age and to improve knowledge of the etiology of gastrointestinal pathogens using traditional and molecular diagnostic techniques. | 2008 | 18523397 |
| detection and surveillance of waterborne protozoan parasites. | the majority of the world's population still live without access to healthy water and the contamination of drinking water with protozoan pathogens poses a serious threat to millions of people in the developing world. even in the developed world periodic outbreaks of diarrhoeal diseases are caused by the protozoan parasites cryptosporidium sp., giardia duodenalis and entamoeba histolytica. thus, surveillance of drinking water is imperative to minimize such contaminations and ensure continuous sup ... | 2008 | 18524569 |
| differences in complement-mediated killing of entamoeba histolytica between men and women--an explanation for the increased susceptibility of men to invasive amebiasis? | men are more than 7 times more likely to develop amebic liver abscess or amebic dysentery caused by entamoeba histolytica than women. because the complement system could play a key role in controlling amebiasis, we determined whether serum from men and women differ in the ability to kill amebic trophozoites. we found that serum from women was significantly more effective in killing e. histolytica trophozoites than serum from men, and this killing was complement dependent. our results provide a p ... | 2008 | 18541770 |
| protozoan protein tyrosine phosphatases. | the aim of this review is to provide a synthesis of the published experimental data on protein tyrosine phosphatases from parasitic protozoa, in silico analysis based on the availability of completed genomes and to place available data for individual phosphatases from different unicellular parasites into the comparative and evolutionary context. we analysed the complement of protein tyrosine phosphatases (ptp) in several species of unicellular parasites that belong to apicomplexa (plasmodium; cr ... | 2008 | 18547579 |
| comparison of two immunoassays for detection of entamoeba histolytica. | effective diagnostic tools are essential in order to combat disease caused by the parasite entamoeba histolytica. in this study, we compared the commercially available ridascreen entamoeba test (r-biopharm) and the e. histolytica ii test (techlab), and we found that the e. histolytica ii test detects e. histolytica infections more accurately. | 2008 | 18550742 |
| differential induction of immunoregulatory circuits of phagocytic cells by gal/gal nac lectin from pathogenic and nonpathogenic entamoeba. | amoebic liver abscess (ala) is an acute inflammatory disease caused by entamoeba histolytica. | 2008 | 18551358 |
| intriguing parasites and intramembrane proteases. | rhomboid intramembrane proteases occur throughout the kingdoms of life. in this issue of genes & development, baxt and colleagues (pp. 1636-1646) report that the single proteolytic rhomboid (ehrom1) from entamoeba histolytica cleaves cell surface galactose-binding or n-acetylgalactosamine-binding (gal/gal-nac) lectins. ehrom1 and lectins colocalize during phagocytosis and receptor capping. ehrom1 is found at the base of the cap rather than in the cap proper, suggesting a role in receptor sheddin ... | 2008 | 18559471 |
| an entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion. | rhomboid proteases are membrane-embedded enzymes conserved in all kingdoms of life, but their cellular functions across evolution are largely unknown. prior work has uncovered a role for rhomboid enzymes in host cell invasion by malaria and related intracellular parasites, but this is unlikely to be a widespread function, even in pathogens, since rhomboid proteases are also conserved in unrelated protozoa that maintain an extracellular existence. we examined rhomboid function in entamoeba histol ... | 2008 | 18559479 |
| the prevalence of intestinal parasites and nasal s. aureus carriage among food handlers. | food handlers play a major role in the transmission of foodborne diseases. nasal staphylococcus aureus (s. aureus) carriage and intestinal parasitism are important risk factors in contamination. the purpose of the authors' study was to determine the prevalence of intestinal parasites and nasal s. aureus carriage among food handlers in manisa, turkey. the authors investigated 8,895 people for nasal s. aureus carriage and intestinal parasites. nasal swab materials and stool samples were examined, ... | 2008 | 18561571 |
| detection of entamoeba histolytica antigen in stool samples in mersin, turkey. | entameoba histolytica, 1 of the 2 entamoeba species with similar morphology that infect humans, causes invasive intestinal and extraintestinal diseases, whereas entamoeba dispar is found commensally and is noninvasive. because of their morphologic similarity, e. histolytica and e. dispar cannot be differentiated microscopically. the antigens of e. histolytica and e. dispar, however, may be detected by the elisa method. previous studies have found that the detection of antigens in the stool is as ... | 2008 | 18564756 |
| a case report: entamoeba histolytica infections in the rhesus macaque, china. | a case of amebic dysentery due to a natural infection of entamoeba histolytica in the rhesus macaque (macaca mullata) was reported. a fecal specimen was isolated and identified by the polymerase chain reaction technique. a daily dose of 750 mg of metronidazole was given orally for 10 days, and good results were observed. the early diagnosis of an e. histolytica infection allowed a proper antiamoebic treatment in an early stage of infection resulting in a successful outcome after therapy. | 2008 | 18566832 |
| molecular epidemiology of amebiasis. | entamoeba histolytica, the causative agent of human amebiasis, remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. entamoeba dispar, morphologically indistinguishable from e. histolytica, is more common in humans in many parts of the world. similarly entamoeba moshkovskii, which was long considered to be a free-living ameba, is also morphologically identical to e. histolytica and e. dispar, and is highly ... | 2008 | 18571478 |
| degradation of human secretory iga1 and iga2 by entamoeba histolytica surface-associated proteolytic activity. | the protozoan entamoeba histolytica is the etiological agent of amebiasis, an infection with high prevalence worldwide. the host-ameba relationship outcome depends on parasite and host factors, and among these is secretory iga. these antibodies reduce mucosal colonization by pathogens and neutralize a variety of toxins and enzymes. the functionality of secretory iga depends on its integrity. some bacteria produce iga proteases that cleave mainly the iga1 subclass; live e. histolytica trophozoite ... | 2008 | 18571975 |
| assessment of entamoeba histolytica antigen in stool samples in mersin. | entameoba histolytica, one of the two entamoeba species with similar morphology that infect humans, causes invasive intestinal and extra-intestinal diseases, whereas entamoeba dispar is found commensally and is non-invasive. due to their morphologic similarity, e. histolytica and e. dispar cannot be differentiated microscopically. the antigens of e. histolytica and e. dispar may be detected by the elisa method. previous studies have found that the detection of antigens in the stool is as sensiti ... | 2008 | 18576733 |
| cockroaches as carriers of human intestinal parasites in two localities in ethiopia. | a study was undertaken to assess the role of cockroaches as potential carriers of human intestinal parasites in addis ababa and ziway, ethiopia. a total of 6480 cockroaches were trapped from the two localities from october 2006 to march 2007. all the cockroaches trapped in addis ababa (n=2240) and almost 50% (2100/4240) of those trapped in ziway were identified as blattella germanica. the rest of the cockroaches trapped in ziway were identified as periplaneta brunnea (24.52%), pycnoscelus surina ... | 2008 | 18579170 |
| entamoeba histolytica: lipid rafts are involved in adhesion of trophozoites to host extracellular matrix components. | adhesion is an important virulence function for entamoeba histolytica, the causative agent of amoebic dysentery. lipid rafts, cholesterol-rich domains, function in compartmentalization of cellular processes. in e. histolytica, rafts participate in parasite-host cell interactions; however, their role in parasite-host extracellular matrix (ecm) interactions has not been explored. disruption of rafts with a cholesterol extracting agent, methyl-beta-cyclodextrin (mbetacd), resulted in inhibition of ... | 2008 | 18588878 |
| cost-effectiveness of detection of intestinal amebiasis by using serology and specific-amebic-antigen assays among persons with or without human immunodeficiency virus infection. | among 345 persons who underwent indirect hemagglutination (iha) serological assays and assays of specific amebic antigens in their stool samples, 24 of 36 (66.7%) who were seropositive for entamoeba histolytica had intestinal amebiasis as determined by antigen assays compared with 2 of 309 (0.2%) who were seronegative (odds ratio, 307; 95% confidence interval, 64.9 to 1,451). the estimated cost to detect a case of intestinal amebiasis by serology followed by antigen assays ($52) could be reduced ... | 2008 | 18596142 |
| invasive amebiasis in men who have sex with men, australia. | entamoeba histolytica is a pathogenic ameba that has recently been recognized as an emerging pathogen in men who have sex with men (msm) in asia-pacific countries where it is not endemic, i.e., japan, taiwan, and republic of korea. we report locally acquired invasive amebiasis in sydney, australia, exclusively in msm. | 2008 | 18598643 |
| putative dead and dexh-box rna helicases families in entamoeba histolytica. | rna helicases catalyze the unwinding of double-stranded rna structures to perform numerous genetic processes. these enzymes are characterized by the presence of a conserved helicase domain with specific helicases motifs whose amino acid sequence allows the differentiation between dead and dexh-box rna helicase families. taking advantage of the availability of the complete genome sequence of entamoeba histolytica, the protozoan responsible for human amoebiasis, we have performed a genomic survey ... | 2008 | 18760338 |
| mutation detection analysis of a region of 16s-like ribosomal rna gene of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii. | the level of intra-species genetic variation in entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii populations in a localized geographic area, like puducherry, india, remains unknown. | 2008 | 18822136 |
| intestinal parasitosis among subjects undergoing cataract surgery at the eye camps in rural hilly areas of nepal. | present study investigated the intestinal parasitosis among 221 subjects undergoing cataract surgery (m: 129 and f: 92; aged 13-86 years) at the eye camps in three rural hilly districts (ramechhap, sindhupalchok and dhading) in 2006. stool samples collected in clean, dry, screw capped plastic containers were examined locally by direct smear technique and anti-parasitic drugs were distributed to parasite positive subjects. the remaining stool samples were fixed with 10% formal-saline, transported ... | 2008 | 18828431 |
| amebiasis. | amebiasis is defined as infection with entamoeba histolytica, regardless of associated symptomatology. in resource-rich nations, this parasitic protozoan is seen primarily in travelers to and emigrants from endemic areas. infections range from asymptomatic colonization to amebic colitis and life-threatening abscesses. importantly, disease may occur months to years after exposure. although e histolytica was previously thought to infect 10% of the world's population, 2 morphologically identical bu ... | 2008 | 18828976 |
| inhibitory effects of iranian thymus vulgaris extracts on in vitro growth of entamoeba histolytica. | one of the most common drugs used against a wide variety of anaerobic protozoan parasites is metronidazole. however, this drug is mutagenic for bacteria and is a potent carcinogen for rodents. thymus vulgaris is used for cough suppression and relief of dyspepsia. also it has antibacterial and antifungal properties. the aim of this study was to investigate antiamebic effect of thymus vulgaris against entamoeba histolytica in comparison with metronidazole. one hundred gram air-dried t. vulgaris pl ... | 2008 | 18830054 |
| thermal unfolding of triosephosphate isomerase from entamoeba histolytica: dimer dissociation leads to extensive unfolding. | in mesophiles, triosephosphate isomerase (tim) is an obligated homodimer. we have previously shown that monomeric folding intermediates are common in the chemical unfolding of tim, where dissociation provides 75% of the overall conformational stability of the dimer. however, analysis of the crystallographic structure shows that, during unfolding, intermonomeric contacts contribute to only 5% of the overall increase in accessible surface area. in this work several methodologies were used to chara ... | 2008 | 18837510 |
| investigation of the prevalence of amoebiasis in izmir province and determination of entamoeba spp. using pcr and enzyme immunoassay. | amoebiasis is a common and life-threatening disease. the discrimination of the pathogenic entamoeba histolytica from the non-pathogenic entamoeba dispar could be done by advanced methods such as enzyme immunoassay (eia) and pcr. the aim of this study was to investigate the prevalence of amoebiasis in izmir province, and differentiate the entamoeba species by pcr and eia. stool samples of 2,047 individuals were examined by direct microscopy, formalin ethyl acetate concentration, trichrome stainin ... | 2008 | 18843895 |
| entamoeba histolytica: response of the parasite to metronidazole challenge on the levels of mrna and protein expression. | entamoeba histolytica remains a cause of significant morbidity and mortality in many countries. although metronidazole has been used for the treatment of amoebiasis for several decades, little is known on how the amoebae react to this challenge on the levels of mrna and protein expression. in this study, we examined their response using a focused microarray, quantitative rt-pcr, and two-dimensional gel electrophoresis (2de). the amoebae modestly increased the levels of mrna coding for superoxide ... | 2008 | 18845147 |
| targets of the entamoeba histolytica transcription factor ure3-bp. | the entamoeba histolytica transcription factor upstream regulatory element 3-binding protein (ure3-bp) is a calcium-responsive regulator of two e. histolytica virulence genes, hgl5 and fdx1. ure3-bp was previously identified by a yeast one-hybrid screen of e. histolytica proteins capable of binding to the sequence tattctatt (upstream regulatory element 3 (ure3)) in the promoter regions of hgl5 and fdx1. in this work, precise definition of the consensus ure3 element was performed by electrophoret ... | 2008 | 18846235 |
| gigantic hepatic amebic abscess presenting as acute abdomen: a case report. | amebiasis is a parasitic disease caused by entamoeba histolytica. it most commonly results in asymptomatic colonization of the gastrointestinal tract, but some patients develop intestinal invasive or extra-intestinal diseases. liver abscess is the most common extra-intestinal manifestation. the large number of clinical presentations of amebic liver abscess makes the diagnosis very challenging in non-endemic countries. late diagnosis of the amebic abscess may lead to perforation and amebic perito ... | 2008 | 18847505 |
| a new modified technique for concentrating intestinal parasites. | the present study evaluated the diagnostic performance of a modification of the formol ethyl acetate concentration technique, with the addition of 25% acetic acid as compared with formol ethyl acetate concentration technique (fea) and fecal parasite concentrator kit fresh fecal material, free of ova and parasites, was pooled in a ratio of 1:4 with 10% buffered formalin to prepare a standardized specimen. sufficient volumes of formalin-fixed suspension of giardia lamblia cysts, entamoeba histolyt ... | 2008 | 18853623 |
| cytomegalovirus colitis mimics amebic colitis in a man with aids. | opportunistic gastrointestinal infections are common in patients with hiv infection; both amebic colitis and cytomegalovirus (cmv) colitis are common causes of chronic diarrhea. it is difficult to distinguish these 2 diseases by nonspecific clinical symptoms such as diarrhea, abdominal pain, and weight loss. here we report a case of cmv colitis mimicking amebic colitis with elevated indirect hemagglutination assay antibody titer against entamoeba histolytica and negative igm antibody titer again ... | 2008 | 18854684 |
| crystallization and preliminary x-ray analysis of l-methionine gamma-lyase 1 from entamoeba histolytica. | l-methionine gamma-lyase (mgl) is a pyridoxal phosphate-dependent enzyme that is involved in the degradation of sulfur-containing amino acids. mgl is an attractive drug target against amoebiasis because the mammalian host of its causative agent entamoeba histolytica lacks mgl. for the development of anti-amoebic agents based on the structure of mgl, one of two mgl isoenzymes (ehmgl1) was crystallized in the monoclinic space group p2(1), with unit-cell parameters a = 99.12, b = 85.38, c = 115.37 ... | 2008 | 18678935 |
| amebic colitis in an antigenically and serologically negative patient: usefulness of a small-subunit ribosomal rna gene-based polymerase chain reaction in diagnosis. | specific identification of entamoeba histolytica in clinical specimens is an essential confirmatory diagnostic step in the management of amebiasis. here, we report an unusual case of amebic colitis in a 20-year-old female immigrant from south china. the patient had experienced diarrhea, crampy abdominal pain, and fever for approximately 3 weeks prior to admission to hospital and had treated herself at home with metronidazole. on admission, stool microscopy and serology for e. histolytica were ne ... | 2008 | 18691843 |
| "inject-mix-react-separate-and-quantitate" (imresq) method for screening enzyme inhibitors. | many regulatory enzymes are considered attractive therapeutic targets, and their inhibitors are potential drug candidates. screening combinatorial libraries for enzyme inhibitors is pivotal to identifying hit compounds for the development of drugs targeting regulatory enzymes. here, we introduce the first inhibitor screening method that consumes only nanoliters of the reactant solutions and is applicable to regulatory enzymes. the method is termed inject-mix-react-separate-and-quantitate (imresq ... | 2008 | 18702487 |
| c1q- and collectin-dependent phagocytosis of apoptotic host cells by the intestinal protozoan entamoeba histolytica. | entamoeba histolytica, the cause of invasive amebiasis, phagocytoses apoptotic host cells during tissue invasion. in mammals, collectin family members (e.g., mannose-binding lectin [mbl]) and the structurally related protein c1q bind to apoptotic cells and stimulate macrophage phagocytosis via a conserved collagenous tail domain. the collectins also bind to bacteria, the usual source of nutrients for e. histolytica. | 2008 | 18702607 |
| prostaglandin e2 produced by entamoeba histolytica binds to ep4 receptors and stimulates interleukin-8 production in human colonic cells. | entamoeba histolytica pathogenesis in the colon occurs in a stepwise fashion. it begins with colonization of the mucin layer, which is followed by stimulation of a proinflammatory response that causes nonspecific tissue damage that may facilitate parasite invasion of the underlying colonic mucosa. unfortunately, the parasite and/or host factors that stimulate a proinflammatory response in the gut are poorly understood. in this study, we found that live e. histolytica or secretory or proteins (sp ... | 2008 | 18710858 |
| entamoeba shows reversible variation in ploidy under different growth conditions and between life cycle phases. | under axenic growth conditions, trophozoites of entamoeba histolytica contain heterogenous amounts of dna due to the presence of both multiple nuclei and different amounts of dna in individual nuclei. in order to establish if the dna content and the observed heterogeneity is maintained during different growth conditions, we have compared e. histolytica cells growing in xenic and axenic cultures. our results show that the nuclear dna content of e. histolytica trophozoites growing in axenic cultur ... | 2008 | 18714361 |
| [epidemiologic and molecular study of entamoeba histolytica and entamoeba dispar strains in pacients with diarrhea in cumana, sucre state, venezuela]. | an epidemiological and molecular study on e. histolytica and e. dispar was carried out in 428 patients with gastrointestinal symptomatology of diarrhea from different health centers in cumana, sucre state. the samples were processed through: direct examination with 0.85% physiological saline solution, temporal lugol staining, trichromic staining and the ritchie method of concentration; a sucrose gradient was used for cyst isolation. the small subunit of the 16s rna was amplified by nested, multi ... | 2008 | 18717268 |
| [amebiasis: importance of the diagnosis and treatment. minireview]. | the protozoa e. histolytica is the etiologic agent of human amebiasis, the identification of its pathogenicity has been of great aid in the search of important antigens for immunodiagnostic and inmunoprofilaxis. in 1995 the isolation and axenization of the first two strains of venezuelan e. histolytica were described from symptomatic patients, which were denominated: iula 1092:1 and iula: 0593:2 these strains have been evaluated through studies of electrophoretic profiles, demonstrating the pres ... | 2008 | 18717272 |
| crusaders' dysentery. | 2008 | 18718438 | |
| ulcers and the colon: keep an open mind. | 2008 | 18719135 | |
| new insights into entamoeba histolytica pathogenesis. | entamoeba histolytica is an important global pathogen and a leading cause of parasitic death worldwide. this article summarizes significant research findings over the last year. | 2008 | 18725798 |
| bursera fagaroides, effect of an ethanolic extract on ornithine decarboxylase (odc) activity in vitro and on the growth of entamoeba histolytica. | the effect of an ethanolic extract from the stem bark of bursera fagaroides on ornithine decarboxylase (odc) activity in vitro and on the growth of entamoeba histolytica was evaluated. for this purpose, increasing concentrations of the extract, up to 8.0mg/ml, were added to amoeba cultures or odc reaction mixtures, which were incubated at 37 degrees c. metronidazole and g418 were added as controls. after 1.5 and 72 h, the odc activity in vitro and growth, respectively, were determined. results r ... | 2008 | 18501354 |
| participation of rho, rock-2, and gap activities during actin microfilament rearrangements in entamoeba histolytica induced by fibronectin signaling. | in entamoeba histolytica little is known about the microfilament rearrangements formed by actin and abps. fibronectin regulates many aspects of cell behavior involving the actin cytoskeleton and members of the rho family of small gtpases. using trophozoites interacted with fibronectin and glass, we present evidence related with the formation and regulation of different microfilament rearrangements and their cellular distribution, the effect of actin affecting drugs on these arrangements, and on ... | 2008 | 18501645 |
| seroprevalence of entamoeba histolytica infection in female outpatients at a sexually transmitted disease sentinel clinic in tokyo, japan. | from 2003 to 2006, we surveyed the seroprevalence of amoebic infection in female outpatients at a gynecologist's office, which was designated as a sexually transmitted disease sentinel clinic by the tokyo metropolitan government, using an enzyme-linked immunosorbent assay (elisa). the annual rate of anti-entamoeba histolytica (hm-1:imsscl6 strain; hm-1) antibody-positive cases as detected by elisa increased during that period, and anti-chlamydia trachomatis antibodies were detected in 60%, i.e., ... | 2008 | 18503164 |
| amebic cerebral abscess mimicking bacterial meningitis. | we report a case of an amebic brain abscess in a 2-year-old girl, with symptoms mimicking bacterial meningitis with no evidence of disease elsewhere. histological evaluation of the abscess revealed the organisms, and the abscess regressed in response to specific medical treatment. this article reviews the rarity of these abscesses and difficulty in the diagnosis. | 2008 | 18810346 |
| mechanisms of infectious diarrhea. | infectious diarrhea is an important public health problem worldwide. research has provided new insights into the mechanisms of diarrhea caused by various pathogens that are classified as noninflammatory, inflammatory or invasive. these three groups of organisms cause two diarrheal syndromes--noninflammatory diarrhea and inflammatory diarrhea. the noninflammatory diarrheas are caused by enterotoxin-producing organisms such as vibrio cholerae and enterotoxigenic escherichia coli, or by viruses tha ... | 2008 | 18813221 |
| trophozoites of entamoeba histolytica epigenetically silenced in several genes are virulence-attenuated. | the human intestinal parasite entamoeba histolytica causes amoebic colitis and amoebic liver abscesses. three classes of amoebic molecules have been identified as the major virulence factors, the gal/galnac inhibitable lectin that mediates adherence to mammalian cells, the amoebapores which cause the formation of membrane ion channels in the target cells and the cysteine proteinases which degrade the matrix proteins, the intestinal mucus and secretory iga. transcriptional silencing of the amoeba ... | 2008 | 18814693 |
| a survey of amoebic infections and differentiation of an entamoeba histolytica-like variant (jsk2004) in nonhuman primates by a multiplex polymerase chain reaction. | a pathogenic entamoeba histolytica-like variant (jsk2004 strain) with genetic variations and a novel isoenzyme pattern isolated from a de brazza's guenon in a tokyo zoo in japan has previously been documented. in this study, a multiplex polymerase chain reaction (pcr) assay that could distinguish the jsk2004-type e. histolytica-like variant (jsk04-eh-v) from e. histolytica and entamoeba dispar using three newly designed primer sets for amplifying each specific dna fragment from their small-subun ... | 2008 | 18816999 |
| analysis of commercial entamoeba histolytica elisa kits for the detection of entamoeba invadens in reptiles. | entamoeba invadens is pathogenic in multiple reptile species and has caused severe outbreaks in zoos and other facilities worldwide. infections can be difficult to diagnose and to differentiate from other reptilian entamoeba species. the goal of this study was to determine if kits developed to identify the human pathogen entamoeba histolytica could be used to detect e. invadens in reptile species at the maryland zoo. the e. histolytica ii antigen enzyme-linked immunosorbent assay and the prospec ... | 2008 | 18817019 |
| cutaneous amebiasis in pediatrics. | cutaneous amebiasis (ca), which is still a health problem in developing countries, is important to diagnose based on its clinical and histopathologic features. | 2008 | 18936402 |
| aetiology of community-acquired, acute gastroenteritis in hospitalised adults: a prospective cohort study. | the aetiology of severe gastroenteritis leading to hospitalisation in adults frequently remains unclear. our objective was to study the causes and characteristics of community-acquired, acute gastroenteritis in adult hospitalized patients to support the clinical management of these patients. | 2008 | 18940017 |
| the divergent eukaryote trichomonas vaginalis has an m7g cap methyltransferase capable of a single n2 methylation. | eukaryotic rnas typically contain 5' cap structures that have been primarily studied in yeast and metazoa. the only known rna cap structure in unicellular protists is the unusual cap4 on trypanosoma brucei mrnas. we have found that t. vaginalis mrnas are protected by a 5' cap structure, however, contrary to that typical for eukaryotes, t. vaginalis spliceosomal snrnas lack a cap and may contain 5' monophophates. the distinctive 2,2,7-trimethylguanosine (tmg) cap structure usually found on snrnas ... | 2008 | 18957443 |
| protozoa in respiratory pathology: a review. | among the micro-organisms that may affect the respiratory apparatus are the protozoa. the diseases they may give rise to constitute a relatively uncommon group of respiratory ailments with, in the majority of cases, an underlying clinical situation corresponding to states of suppressed immunity (aids, transplants, malign haemopathies, corticotherapy, etc.). other factors, such as visits to endemic areas and immigration, also have to be taken into account. in view of the probable increase in the ... | 2008 | 18978136 |
| first molecular identification of entamoeba moshkovskii in human stool samples in tunisia. | we report the first intestinal infections in tunisia with entamoeba moshkovskii in two healthy adults. entamoeba moshkovskii cysts were distinguished from those of the morphologically identical parasites entamoeba histolytica and entamoeba dispar by specific nested polymerase chain reaction and sequencing. | 2008 | 18981508 |
| three cases of intestinal capillariasis in lao people's democratic republic. | capillaria philippinensis is a rare zoonotic intestinal parasite that emerged in the 1960s. the outcome of intestinal capillariasis may be fatal if untreated in due time. we report three cases of intestinal capillariasis in lao people's democratic republic (lao pdr). the three patients were unrelated previously healthy young men (24, 26, and 27 years of age) with no underlying disease or immune depression. they had chronic diarrhea, abdominal pain, edema, and severe weight loss. two of them acqu ... | 2008 | 18981514 |
| an outbreak of gastroenteritis associated with intestinal parasites. | intestinal protozoa are one of the leading causes of waterborne outbreaks. stool samples of 196 residents from a village of izmir, using the public water supply, were collected during an outbreak of gastroenteritis. patients were asked to fill out a questionnaire reporting on gender, age, gastrointestinal symptoms, whether or not there was a toilet in the house, their hygiene practices, and similar symptoms in the household members. of the patients who had gastrointestinal symptoms (74.5%), diar ... | 2008 | 18985582 |
| [the prevalence of intestinal parasites in children brought to the kars maternal and children's hospital with complaints of gastrointestinal symptoms]. | this study was carried out to determine the prevalence of intestinal parasites in 2-6 year-old children who were brought to kars maternal and children's hospital with complaints of gastrointestinal symptoms during march-june 2007. fecal samples were taken from children and brought to the parasitology laboratory of the faculty of veterinary medicine to be examined for intestinal parasites. fecal samples were examined by centrifugal formalin ether, zinc-sulphate floatation, and modified acid fast ... | 2008 | 18985583 |
| [the investigation of intestinal parasites in two primary schools in different social-economic districts of the city of yozgat, turkey]. | the aim of this study was to determine the incidence of intestinal parasites among children in two primary schools in different social-economic districts in the central region of yozgat and to educate the children about parasitic diseases. during the study, cellophane tape preparations and stool samples that had been prepared using direct mounting methods were examined. in addition the students were informed about intestinal parasites. parasitic infection was observed in 128 (34.9%) out of 367 s ... | 2008 | 18985585 |
| [distribution of intestinal parasites in patients presenting at the parasitology laboratory of the medical school of erciyes university between the years of 2005 and 2008]. | in this study carried out from january 2005-june 2008, a total of 28,911 stool samples were examined using native-lugol and flotation/sedimentation methods and 7,164 cellophane tape preparations were examined directly. intestinal parasites were found in 6,975 (24.13%). of the patients, 52.90% were female and 47.10%, male. blastocystis hominis, 5,701 (19.72%); entamoeba coli, 910 (3.15%); giardia intestinalis, 568 (1.96%); endolimax nana, 363 (1.26%); entamoeba hartmann i, 357 (1.23%); entamoeba ... | 2008 | 18985586 |
| transcriptional regulatory networks in entamoeba histolytica. | expression profiling with microarray technology has revolutionized exploration of transcriptional regulatory networks on a genome-wide scale. this approach has been successfully applied to the study of entamoeba histolytica, which causes dysentery and liver abscesses and is a leading parasitic cause of death globally. a variety of microarray platforms have been developed for this system including those generated from genomic dna, long oligonucleotides, and short oligonucleotides. using these too ... | 2008 | 18991605 |
| not only coughs and sneezes. | 2008 | 18994670 | |
| is intestinal parasitic infection still a public health concern among saudi children? | to determine the possible health risk associated with intestinal parasite infections among children with gastroenteritis in jeddah, kingdom of saudi arabia. | 2008 | 18998015 |
| imaging of hepatic infections. | hepatic infections include pyogenic and amebic abscesses and fungal and parasitic diseases. entry of the infectious organisms into the liver can occur by hematogenous spread via the portal vein or hepatic artery, ascension of the infection from the biliary tract, or from trauma. worldwide, liver abscess is most often caused by entamoeba histolytica, but in the developed world, pyogenic liver abscess is more common. fungal infection is most often seen in immunosuppressed chemotherapy patients, wh ... | 2008 | 19060716 |
| detection of entamoeba histolytica dna in the saliva of amoebic liver abscess patients who received prior treatment with metronidazole. | saliva is an easily-accessible and a non-invasive clinical specimen alternate to blood and liver pus. an attempt was made to detect entamoeba histolytica dna released in the saliva of amoebic liver abscess (ala) patients by applying 16s-like rrna gene-based nested multiplex polymerase chain reaction (nm-pcr). the nm-pcr detected e. histolytica dna in the saliva of eight (28.6%) of 28 ala patients. the nm-pcr result was negative for e. histolytica dna in the saliva of all the eight ala patients w ... | 2008 | 19069620 |
| prevalence of bacteria and intestinal parasites among food-handlers in gondar town, northwest ethiopia. | food-handlers with poor personal hygiene working in food-service establishments could be potential sources of infection due to pathogenic organisms. the study was undertaken to determine the prevalence of bacteria and intestinal parasites among 127 food-handlers working in the cafeterias of the university of gondar and the gondar teachers training college, gondar, ethiopia. fingernail contents of both the hands and stool specimens were collected from all the 127 food-handlers. the samples were e ... | 2008 | 19069624 |
| genome wide survey, discovery and evolution of repetitive elements in three entamoeba species. | identification and mapping of repetitive elements is a key step for accurate gene prediction and overall structural annotation of genomes. during the assembly and annotation of three highly repetitive amoeba genomes, entamoeba histolytica, entamoeba dispar, and entamoeba invadens, we performed comparative sequence analysis to identify and map all class i and class ii transposable elements in their sequences. | 2008 | 19077187 |
| persistence of entamoeba histolytica infection in cba mice owes to intestinal il-4 production and inhibition of protective ifn-gamma. | the mechanisms whereby certain mouse strains develop persistent intestinal infection with entamoeba histolytica remain unclear. in this work, we characterized the kinetic pattern of cytokine responses during the course of natural infection in cba mice and showed that intracecal amebic infection led to a rapid and sustained upregulation of th2 (il-4, il-5, il-13) and th17 cytokine responses while th1 cytokines, il-12p35 and interferon (ifn)-gamma, were suppressed. depletion of il-4 cleared infect ... | 2008 | 19079171 |
| innate immunity prevents tissue invasion by entamoeba histolytica. | although innate and adaptive immunity both play a role in amoebiasis, the mechanisms involved in the elimination of entamoeba histolytica are poorly understood. to provide more information about the innate immune mechanisms that may confer protection against invasive amoebiasis, we administered inflammatory substances (bacillus calmette-guérin, lipopolysaccharide, complete freund's adjuvant, or mineral oil) into the peritoneum of hamsters. the animals were then challenged with pathogenic trophoz ... | 2008 | 19096458 |
| the vacuolar atpase from entamoeba histolytica: molecular cloning of the gene encoding for the b subunit and subcellular localization of the protein. | entamoeba histolytica is a professional phagocytic cell where the vacuolar atpase plays a key role. this enzyme is a multisubunit complex that regulates ph in many subcellular compartments, even in those that are not measurably acidic. it participates in a wide variety of cellular processes such as endocytosis, intracellular transport and membrane fusion. the presence of a vacuolar type h+-atpase in e. histolytica trophozoites has been inferred previously from inhibition assays of its activity, ... | 2008 | 19108705 |
| a case report of pulmonary amoebiasis with entamoeba histolytica diagnosed in western romania. | among parasitic diseases, amoebiasis is the third most frequent cause of mortality after malaria and schistosomiasis. pulmonary amoebiasis, the second most common extraintestinal manifestation of infection, occurs in 2%-3% of patients with invasive amoebiasis. the present clinical report aims to present a single case of a rare diagnosed parasitic disease in romania and to emphasize the difficulties encounted while attempting to establish the correct diagnosis and to completely cure the patient. | 2008 | 19745512 |
| [prevalence and polyparasitism of intestinal protozoa and spatial distribution of entamoeba histolytica, e. dispar and giardia intestinalis from pupils in the rural zone of man in côte d'ivoire]. | diseases caused by environmental contamination by micro-organisms, including intestinal helminths and protozoa, are prevalent in developing countries. according to some authors, their strong expansion in some zones of these countries is due primarily to favourable climatic conditions, combined with inadequate hygiene measures and cleaning and the generally low socio-economic level. progress in disease control has resulted from new studies that improve our understanding of the epidemiology of hel ... | 2008 | 19810617 |
| entamoeba dispar: genetic diversity of iranian isolates based on serine-rich entamoeba dispar protein gene. | the nucleotide sequences of serine-rich entamoeba histolytica protein (srehp) gene have already exhibited stable and significant polymorphism in the gene studies. serine-rich protein is also present and polymorphic in entamoeba dispar which called sredp. the polymorphism of the serine-rich entamoeba dispar protein (sredp) gene among 8 isolates obtained from iranian cyst carriers were analyzed by a nested pcr-rflp followed by sequencing of the pcr products. from those isolates, six distinct dna p ... | 2008 | 19630212 |
| sequence specific 1h, 13c and 15n resonance assignments of a calmodulin-like calcium-binding protein from the protozoan parasite entamoeba histolytica (ehcam). | we report almost complete sequence specific 1h, 13c and 15n nmr assignments of a 151-residue long calmodulin-like calcium-binding protein from entamoeba histolytica (ehcam). | 2008 | 19636930 |
| endoplasmic reticulum continuity in the protozoan parasite entamoeba histolytica: evolutionary implications and a cautionary note. | entamoeba histolytica has been described as an early branching eukaryotic parasite based on the lack of organelles such as mitochondria and peroxisomes, and on morphologic studies that concluded it possesses a vesicular endoplasmic reticulum (er) and golgi complex. however, a recent study from our laboratory showed that the e. histolytica er is continuous by using an er-targeted green fluorescent protein fusion protein and photobleaching experiments. we proposed that the vesicular er seen earlie ... | 2008 | 19704884 |
| comparison of clinical characteristics of amebic liver abscess in human immunodeficiency virus (hiv)-infected and non-hiv-infected patients. | amebic liver abscess is an emerging parasitic disease in human immunodeficiency virus (hiv)-infected patients. clinical characteristics of this disease have rarely been compared between patients with and without hiv infection. | 2008 | 19255688 |
| intestinal parasitosis in nalout popularity, western libya. | this paper studied gastrointestinal parasitosis in nalout popularity. a hospital based study was done on a total of 800 stool samples from a private laboratory in tigi city. the results showed an overall rate of 29.6%. of these patients, 7.59% had double or triple parasitic infections. children were more infected than adults. the parasites in a descending order were entamoeba histolytica (21%), enterobius vermicularis (7.5%), giardia lamblia (2.5%), balantidium coli (0.38%), and hymenolepis nana ... | 2008 | 19143135 |
| parasitiosis in handicapped children in an egyptian blind asylum. | examination of visually handicapped children and their serving staff revealed in a descending order enterobius vermicularis, giardia lamblia, entamoeba histolytica, hymenolepis nana, ascaris lumbricoides, cryptospodium parvum, tricocephalus trichura and strongyloides stercoralis. head lice were encountered. but, neither blood parasites nor protozoan infective agent in water for human consumption. | 2008 | 19143142 |
| small rnas with 5'-polyphosphate termini associate with a piwi-related protein and regulate gene expression in the single-celled eukaryote entamoeba histolytica. | small interfering rnas regulate gene expression in diverse biological processes, including heterochromatin formation and dna elimination, developmental regulation, and cell differentiation. in the single-celled eukaryote entamoeba histolytica, we have identified a population of small rnas of 27 nt size that (i) have 5'-polyphosphate termini, (ii) map antisense to genes, and (iii) associate with an e. histolytica piwi-related protein. whole genome microarray expression analysis revealed that esse ... | 2008 | 19043551 |
| ipecacuanha: the south american vomiting root. | the story of ipecacuanha, derived from the plant cephaelis, is a fascinating one. it was discovered in brazil in the 1600s and then transported to paris in the latter part of the same century. it was used there by the physician helvetius on various members of the french royal court to treat the flux (dysentery) with some success. later, in the eighteenth century, it was taken up by the physician and privateer thomas dover and became, with opium, a fundamental constituent of his celebrated powder ... | 2008 | 19227966 |