Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the effect on dental caries of topically applied acidulated phosphate-fluoride: results after one year. | 2015 | 4384484 | |
| sewage treatment by means of oxidation ponds. | 2015 | 4432066 | |
| diagnostic abdominal radioisotopic lymphography and radiation therapy. | 2015 | 4333860 | |
| the n.y. dental service corp.--present status. a report. | 2015 | 4519492 | |
| concentration of cyclic 3':5'-adenosine monophosphate and glycogen metabolism in the liver. | 2015 | 4319507 | |
| n-terminal aspartate, glycine and serine in poliovirus capsid protein. | 2015 | 4357935 | |
| [connective tissue tumors of the soft parts (apropos of 30 cases)]. | 2015 | 4334588 | |
| [q beta rna replicase]. | 2015 | 4567707 | |
| dark recovery of erp in isolated octopus retina. | 2015 | 4648395 | |
| mass spectra of biologically active indole, 1-methylindole, and benzo(b)thiopene derivatives. | 2015 | 4824826 | |
| mass spectra of biologically active indole, 1-methylindole, and benzo(b)thiopene derivatives. | 2015 | 4824826 | |
| agglutination of labeled rbc. | 2015 | 4623528 | |
| [functional disorders of the biliary tracts]. | 2015 | 4553777 | |
| the response of chelonian muscle spindles to mechanical stimulation. | 2015 | 4549902 | |
| [hypothalamic monoamine nerve fiber and sexual mechanism control]. | 2015 | 4601360 | |
| [myocardial lesions in progressive muscular dystrophy]. | 2015 | 4467460 | |
| participation of monocytes in transient glomerular hypercellularity in poststreptococcal glomerulonephritis. | 2015 | 4350411 | |
| nursing education: "the clarnnda plan". | 2015 | 4322725 | |
| multicentric reticulohistiocytosis with extensive bone and joint destruction. | 2015 | 4518826 | |
| nursing ethics: the admirable professional standards of nurses. a survey report. | 2015 | 4496571 | |
| metastatic calcification detected through scanning with 99mtc-polyphosphate. | 2015 | 4424278 | |
| prevalence and genetic diversity of clinical vibrio parahaemolyticus isolates from china, revealed by multilocus sequence typing scheme. | the population structure of clinical vibrio parahaemolyticus isolates spreading in china remains undefined. we brought 218 clinical isolates from the pubmlst database originating from different regions of china collected since the year of 1990, analyzed by multilocus sequence typing (mlst), to elucidate the prevalence and genetic diversity of v. parahaemolyticus circulating in chinese population. the mlst scheme produced 137 sequence types (sts). these sts were clustered into six clonal complexe ... | 2015 | 25914691 |
| comprehensive analysis of a vibrio parahaemolyticus strain extracellular serine protease vpsp37. | proteases play an important role in the field of tissue dissociation combined with regenerative medicine. during the years new sources of proteolytic enzymes have been studied including proteases from different marine organisms both eukaryotic and prokaryotic. herein we have purified a secreted component of an isolate of vibrio parahaemolyticus, with electrophoretic mobilities corresponding to 36 kda, belonging to the serine proteases family. sequencing of the n-terminus enabled the in silico id ... | 2015 | 26162075 |
| a novel adhesive factor contributing to the virulence of vibrio parahaemolyticus. | bacterial adhesins play a pivotal role in the tight bacteria-host cells attachment to initiate the downstream processes and bacterial infection of hosts. in this study, we identified a novel adhesin, vpadf in v. parahaemolyticus. deletion of vpadf in v. parahaemolyticus markedly impaired its attachment and cytotoxicity to epithelial cells, as well as attenuated the virulence in murine model. biochemical studies revealed that vpadf recognized both fibronectin and fibrinogen. the binding of vpadf ... | 2015 | 26399174 |
| [total ligation of the esophagus by murphy's button. hemmorrhages caused by esophageal varices in portal hypertension. apropos of 34 cases]. | 2015 | 4549250 | |
| observations on pulmonary function testing and blood-gas analysis in children. | 2015 | 4503927 | |
| [the activity of factor xiii in plasma of children with acute leucaemia (author's transl)]. | 2015 | 4518176 | |
| [caucasian period of the activity of v. s. bobrovskiĭ (on the 100th anniversary of his birth)]. | 2015 | 4592872 | |
| identification, expression, and innate immune responses of two insulin-like peptide genes in the razor clam sinonovacula constricta. | insulin-like peptide (ilp) has emerged as a cell regulatory factor with multiple functions in vertebrates and invertebrates. in the present study, we identified and characterized two ilp genes, ilp1 and ilp2, in the razor clam sinonovacula constricta. both ilps have a signal peptide and a mature domain consisting of six strictly conserved cysteines. the tertiary structure is divided into three main α-helices with a c-domain loop that separates helix 1 from helix 2. both of ilps were found to be ... | 2016 | 26980611 |
| lipopolysaccharide and beta-1, 3-glucan binding protein (lgbp) stimulates prophenoloxidase activating system in chinese mitten crab (eriocheir sinensis). | melanization mediated by prophenoloxidase (propo) activating system play an essential role in killing invading microorganisms in invertebrates. lipopolysaccharide and β-1, 3-glucan binding protein (lgbp) as a pattern recognition protein have been demonstrated to active the propo cascade in insect and shrimp. in this study, we investigated the role of lgbp in prophenoloxidase cascade-induced melanization in chinese mitten crab (eriocheir sinensis). by rt-pcr analysis, eslgbp was detected in all t ... | 2016 | 26995767 |
| mkk6 from pacific white shrimp litopenaeus vannamei is responsive to bacterial and wssv infection. | p38 mitogen-actived protein kinases (mapks) broadly exist from yeast to mammals and participate in diverse cellular responses to various stimuli, whose activation can be induced by the mapk kinase 6 (mkk6). in this study, a novel mkk6 homolog from litopenaeus vannamei (lvmkk6) was cloned and characterized. the transcript of lvmkk6 was 1465bp long with an open reading frame (orf) of 987bp that encoded a polypeptide of 328 amino acids. lvmkk6 was a both cytoplasmic- and nuclear-localized protein a ... | 2016 | 26745320 |
| a centrifugal direct recombinase polymerase amplification (direct-rpa) microdevice for multiplex and real-time identification of food poisoning bacteria. | in this study, we developed a centrifugal direct recombinase polymerase amplification (direct-rpa) microdevice for multiplex and real-time identification of food poisoning bacteria contaminated milk samples. the microdevice was designed to contain identical triplicate functional units and each unit has four reaction chambers, thereby making it possible to perform twelve direct-rpa reactions simultaneously. the integrated microdevice consisted of two layers: rpa reagents were injected in the top ... | 2016 | 27216297 |
| identification and function of a novel c1q domain-containing (c1qdc) protein in triangle-shell pearl mussel (hyriopsis cumingii). | c1q is the target recognition sequence of the classical complement pathway and a major link that connects innate and acquired immunity. in this study, a c1qdc homolog, hcc1qdc5, from the triangle-shell pearl mussel (hyriopsis cumingii) was identified. the complete nucleotide sequence of hcc1qdc5 cdna consists of a 5'-untranslated terminal region (utr) of 123 bp, a 3'-utr of 105 bp with a poly(a) tail, and an open reading frame (orf) of 1344 bp, which encodes a polypeptide of 447 amino acids. hcc ... | 2016 | 27725260 |
| identification and characterization of transforming growth factor β-activated kinase 1 from litopenaeus vannamei involved in anti-bacterial host defense. | lvtak1, a member of transforming growth factor β-activated kinase 1 (tak1) families, has been identified from litopenaeus vannamei in this study. the full length of lvtak1 is 2670 bp, including a 2277 bp open reading frame (orf) that encoded a putative protein of 758 amino acids with a calculated molecular weight of ∼83.4 kda lvtak1 expression was most abundant in muscles and was up-regulated in gills after lps, vibrio parahaemolyticus, staphylococcus aureus, poly (i:c) and wssv challenge. both ... | 2016 | 27033469 |
| role of transglutaminase in immune defense against bacterial pathogens via regulation of antimicrobial peptides. | transglutaminase (tgase) is critical for blood coagulation, a conserved immunological defense mechanism among invertebrates. here, a 3248-bp (full-length) tgase cdna in eriocheir sinensis (estgase) was cloned, with a 2274-bp open reading frame (orf) encoding a 757 amino acid protein containing two transglut domains, one tgase/protease-like homolog domain and a kgd (lys-gly-asp) motif. phylogenetic analysis demonstrated that estgase appeared earlier in evolution compared with tgases of other crus ... | 2016 | 26464201 |
| molecular detection of the three major pathogenic vibrio species from seafood products and sediments in tunisia using real-time pcr. | vibrio spp. have emerged as a serious threat to human health worldwide. v. parahaemolyticus , v. cholerae , and v. vulnificus pose a considerable public health risk in tunisia because they cause sporadic and epidemic foodborne infections associated with the consumption of raw or undercooked contaminated seafood. more recently, toxr-positive v. alginolyticus was also reported to be a potential source of contaminated seafood. a total of 247 samples, including 113 fishes ( labrus viridis , penaeus ... | 2016 | 28221954 |
| characterization of vibrio parahaemolyticus and its specific phage from shrimp pond in palk strait, south east coast of india. | phage therapy is an alternative and eco-friendly biocontrol agent to prevent and control multidrug resistant bacteria in the aquatic system. the aim of this study is to isolate and characterize the vibrio parahaemolyticus and its potential lytic phage from penaeus monodon growing-out by rearing in shrimp ponds in palk strait, south east coast of india. the conventional phenotypic characteristics and molecular identification was confirmed using 16s rrna sequence and to determine the antibiotic re ... | 2016 | 27697363 |
| isolation and characterization of agar-digesting vibrio species from the rotten thallus of gracilariopsis heteroclada zhang et xia. | gracilariopsis heteroclada zhang et xia (gracilariaceae, rhodophyta) is one of the most studied marine seaweeds due to its economic importance. this has been cultivated extensively on commercial scale in the philippines and other asian countries. however, sustainable production of g. heteroclada in the philippines could not be maximized due to the occurrence of rotten thallus disease. thus, isolation and characterization of agar-digesting bacteria from the rotten thalli of g. heteroclada was con ... | 2016 | 27285614 |
| effects of methanolic macroalgae extracts from caulerpa sertularioides and ulva lactuca on litopenaeus vannamei survival in the presence of vibrio bacteria. | macroalgae are potentially excellent sources of highly bioactive secondary metabolites that are useful for the development of new functional ingredients. this study was conducted to determine whether methanolic extracts from caulerpa sertularioides and ulva lactuca macroalgae might be possible alternatives for the prevention of shrimp vibriosis, which is caused by vibrio parahaemolyticus and vibrio alginolyticus. macroalgae extracts prepared with methanol as the solvent were evaluated for antiba ... | 2016 | 26915309 |
| role of non-coding regulatory rna in the virulence of human pathogenic vibrios. | in recent decades, the identification of small non-coding rnas in bacteria has revealed an important regulatory mechanism of gene expression involved in the response to environmental signals and to the control of virulence. in the family vibrionaceae, which includes several human and animal pathogens, small non-coding rnas (srnas) are closely related to important processes including metabolism, quorum sensing, virulence, and fitness. studies conducted in silico and experiments using microarrays ... | 2016 | 28123382 |
| occurrence of vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus in the clam ruditapes philippinarum (adams & reeve, 1850) from emilia romagna and sardinia, italy. | marine vibrios, vibrio parahaemolyticus, v. vulnificus and v. cholerae are responsible of the majority of food-borne human infections by consumption of bivalve shellfish. the aim of the present study was to ascertain the occurrence of these bacteria, and their potential pathogenicity, in the manila clam r. philippinarum from emilia romagna (er) and sardinia (sr) regions, italy. isolation was performed on chromagar(tm) vibrio with subculture on (thiosulfate-citrate-bile salts-sucrose) agar and m- ... | 2016 | 27800436 |
| quantification of vibrio species in oysters from the gulf of mexico with two procedures based on mpn and pcr. | oysters can accumulate potentially pathogenic water bacteria. the objective of this study was to compare two procedures to quantify vibrio species present in oysters to determine the most sensitive method. we analyzed oyster samples from the gulf of mexico, commercialized in mexico city. the samples were inoculated in tubes with alkaline peptone water (apw), based on three tubes and four dilutions (10(-1) to 10(-4)). from these tubes, the first quantification of vibrio species was performed (mos ... | 2016 | 27709461 |
| isolation and identification of vibrio cholerae and vibrio parahaemolyticus from prawn (penaeus monodon) seafood: preservation strategies. | bacterial diseases are one of the major problems which affects the production, development and expansion of aqua culture. vibrio sp. are widespread in marine and estuarine environments. the several pathogenic species are commonly associated with outbreaks of vibrio species and it is mainly associated with food poisonings. in this research, the occurrence of vibrio sp. was studied by the isolation and it is confirmed by the biochemical methods. the growth rate was studied by changing the differen ... | 2016 | 27457973 |
| host, pathogen and environment: a bacterial gbpa gene expression study in response to magnesium environment and presence of prawn carapace and commercial chitin. | vibrio parahaemolyticus is a gram-negative halophilic bacterium which is found largely in estuarine and coastal waters. the bacteria has been a main focus in gastro-intestinal infections caused primarily due to the consumption of contaminated seafood. it was shown to survive in magnesium concentrations as high as 300 mm which are toxic to various other micro-organisms. several genes of v. parahaemolyticus were studied, among which gbpa (n-acetyl glucosamine binding protein) was reported in vibri ... | 2016 | 27231485 |
| genetic analysis of vibrio parahaemolyticus intestinal colonization. | vibrio parahaemolyticus is the most common cause of seafood-borne gastroenteritis worldwide and a blight on global aquaculture. this organism requires a horizontally acquired type iii secretion system (t3ss2) to infect the small intestine, but knowledge of additional factors that underlie v. parahaemolyticus pathogenicity is limited. we used transposon-insertion sequencing to screen for genes that contribute to viability of v. parahaemolyticus in vitro and in the mammalian intestine. our analysi ... | 2016 | 27185914 |
| spatiotemporal dynamics of vibrio spp. within the sydney harbour estuary. | vibrio are a genus of marine bacteria that have substantial environmental and human health importance, and there is evidence that their impact may be increasing as a consequence of changing environmental conditions. we investigated the abundance and composition of the vibrio community within the sydney harbour estuary, one of the most densely populated coastal areas in australia, and a region currently experiencing rapidly changing environmental conditions. using quantitative pcr (qpcr) and vibr ... | 2016 | 27148171 |
| detection and quantification of vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus in coastal waters of guinea-bissau (west africa). | v. cholerae, v. parahaemolyticus, and v. vulnificus are recognized human pathogens. although several studies are available worldwide, both on environmental and clinical contexts, little is known about the ecology of these vibrios in african coastal waters. in this study, their co-occurrence and relationships to key environmental constraints in the coastal waters of guinea-bissau were examined using the most probable number-polymerase chain reaction (mpn-pcr) approach. all vibrio species were uni ... | 2016 | 26940502 |
| occurrence and molecular characterisation of vibrio parahaemolyticus in crustaceans commercialised in venice area, italy. | infections due to the pathogenic human vibrios, vibrio parahaemolyticus, vibrio cholerae and vibrio vulnificus, are mainly associated with consumption of raw or partially cooked bivalve molluscs. at present, little is known about the presence of vibrio species in crustaceans and the risk of vibriosis associated with the consumption of these products. the aim of the present study was to evaluate the prevalence and concentration of the main pathogenic vibrio spp. in samples of crustaceans (n=143) ... | 2016 | 26773255 |
| identification of dld, by immunoproteomic analysis and evaluation as a potential vaccine antigen against three vibrio species in epinephelus coioides. | vibrio spp. represent a serious threat to the culture of epinephelus coioides (orange-spotted grouper) in southeast asia. in this study we used two-dimensional electrophoresis (2-de) and western blotting to identify common immunogenic proteins of vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus. membranes were probed with orange-spotted grouper anti-v. alginolyticus sera and accordingly 60, 58 and 48 immunogenic protein spots were detected. by matching analysis for the three west ... | 2016 | 26562319 |
| antibacterial efficacy of 405, 460 and 520 nm light emitting diodes on lactobacillus plantarum, staphylococcus aureus and vibrio parahaemolyticus. | little information is available on a direct comparison of the antibacterial efficacy of light emitting diode (leds) of different peak wavelengths. thus, the objective of this study was to evaluate the effect of leds of three different wavelengths on bacterial inactivation. | 2016 | 26481103 |
| exse is a negative regulator for t3ss gene expression in vibrio alginolyticus. | type iii secretion systems (t3sss) contribute to microbial pathogenesis of vibrio species, but the regulatory mechanisms are complex. we determined if the classic exsacde protein-protein regulatory model from pseudomonas aeruginosa applies to vibrio alginolyticus. deletion mutants in v. alginolyticus demonstrated that, as expected, the t3ss is positively regulated by exsa and exsc and negatively regulated by exsd and exse. interestingly, deletion of exse enhanced the ability of v. alginolyticus ... | 2016 | 27999769 |
| inhibition of marine vibrio sp. by pyoverdine from pseudomonas aeruginosa pa1. | siderophores are low-molecular-weight chemicals that are secreted by many microorganisms to chelate iron from the external environment in order to facilitate their growth and diverse metabolisms. in this study, a fluorescent siderophore, pyoverdine, secreted by pseudomonas aeruginosa pa1 was purified by affinity chromatography using cu-sepharose. pyoverdine was determined to have a molecular mass of 1333.54 da, as determined by maldi-tof/tof, and belong to type i pyoverdine, as determined by pcr ... | 2016 | 26476308 |
| the expression of heterologous mam-7 in lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen vibrio parahaemolyticus in vitro. | vibrio parahaemolyticus (v. parahaemolyticus) is a gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. when ingested, v. parahaemolyticus causes a self-limiting illness (vibriosis), characterized mainly by watery diarrhoea. treatment is usually oral rehydration and/or antibiotics in complicated cases. since 1996, the pathogenic and pandemic v. parahaemolyticus o3:k6 serotype has spread worldwide, increasing the reported number of vibriosis cases. thus, ... | 2016 | 26739707 |
| analysis of microbiota on abalone (haliotis discus hannai) in south korea for improved product management. | abalone is a popular seafood in south korea; however, because it contains various microorganisms, its ingestion can cause food poisoning. therefore, analysis of the microbiota on abalone can improve understanding of outbreaks and causes of food poisoning and help to better manage seafood products. in this study, we collected a total of 40 abalones from four different regions in march and july, which are known as the maximum abalone production areas in korea. the microbiota were analyzed using hi ... | 2016 | 27371902 |
| bacterial and viral pathogens detected in sea turtles stranded along the coast of tuscany, italy. | during 2014, six loggerhead turtles, caretta caretta and one green turtle, chelonia mydas, found stranded on the tuscany coast of italy, were examined for the presence of specific bacterial and viral agents, along with their role as carriers of fish and human pathogens. thirteen different species of bacteria, 10 gram negative and 3 gram positive, were identified. among them, two strains of vibrio parahaemolyticus and one strain of lactococcus garviae were recovered and confirmed by specific pcr ... | 2016 | 26931392 |
| survival of foodborne pathogens (escherichia coli o157:h7, salmonella typhimurium, staphylococcus aureus, listeria monocytogenes, and vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce. | knowing the survival characteristics of foodborne pathogens in raw ready-to-eat (rte) seafood is the key to predicting whether they pose a microbiological hazard. the present study examined the survival of escherichia coli o157:h7, salmonella typhimurium, vibrio parahaemoliticus, listeria monocytogenes, and staphylococcus aureus in raw rte crab marinated in soy sauce. inoculated crabs (initial bacterial population=4.1-4.4logcfu/g) were immersed in soy sauce and then stored at refrigeration (5°c) ... | 2016 | 27591386 |
| three novel c1q domain containing proteins from the disk abalone haliotis discus discus: genomic organization and analysis of the transcriptional changes in response to bacterial pathogens. | the globular c1q (gc1q) domain containing proteins, commonly referred as c1q domain containing (c1qdc) proteins, are an essential family of proteins involved in various innate immune responses. in this study, three novel c1qdc proteins were identified from the disk abalone (haliotis discus discus) transcriptome database and designated as abc1qdc1, abc1qdc2, and abc1qdc3. the cdna sequences of abc1qdc1, abc1qdc2, and abc1qdc3 consisted of 807, 1305, and 660 bp open reading frames (orfs) encoding ... | 2016 | 27417231 |
| fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device. | this work describes fully automated and colorimetric foodborne pathogen detection on an integrated centrifugal microfluidic device, which is called a lab-on-a-disc. all the processes for molecular diagnostics including dna extraction and purification, dna amplification and amplicon detection were integrated on a single disc. silica microbeads incorporated in the disc enabled extraction and purification of bacterial genomic dna from bacteria-contaminated milk samples. we targeted four kinds of fo ... | 2016 | 27112702 |
| a molluscan calreticulin ortholog from haliotis discus discus: molecular characterization and transcriptional evidence for its role in host immunity. | calreticulin (calr), a ca(2+) binding chaperone of the endoplasmic reticulum (er) and mainly involved in ca(2+) storage and signaling. in this study, we report the molecular characterization and immune responses of calr homolog from disk abalone (abcalr). the full length abcalr cdna (1837 bp) had an orf of 1224 bp. according to the multiple alignments analysis, n- and p-domains were highly conserved in all the selected members of calrs. in contrast, the c-domain which terminated with the charact ... | 2016 | 27086846 |
| acidic electrolyzed water as a novel transmitting medium for high hydrostatic pressure reduction of bacterial loads on shelled fresh shrimp. | acidic electrolyzed water (aew), a novel non-thermal sterilization technology, is widely used in the food industry. in this study, we firstly investigated the effect of aew as a new pressure transmitting medium for high hydrostatic pressure (aew-hhp) processing on microorganisms inactivation on shelled fresh shrimp. the optimal conditions of aew-hhp for vibrio parahaemolyticus inactivation on sterile shelled fresh shrimp were obtained using response surface methodology: nacl concentration to ele ... | 2016 | 27014228 |
| lptd is a promising vaccine antigen and potential immunotherapeutic target for protection against vibrio species infection. | outer membrane proteins (omps) are unique to gram-negative bacteria. several features, including surface exposure, conservation among strains and ability to induce immune responses, make omps attractive targets for using as vaccine antigens and immunotherapeutics. lptd is an essential omp that mediates the final transport of lipopolysaccharide (lps) to outer leaflet. the protein in vibrio parahaemolyticus was identified to have immunogenicity in our previous report. in this study, broad distribu ... | 2016 | 27922123 |
| nqrm (duf539) protein is required for maturation of bacterial na+-translocating nadh:quinone oxidoreductase. | na(+)-translocating nadh:quinone oxidoreductase (na(+)-nqr) catalyzes electron transfer from nadh to ubiquinone in the bacterial respiratory chain, coupled with na(+) translocation across the membrane. na(+)-nqr maturation involves covalent attachment of flavin mononucleotide (fmn) residues, catalyzed by flavin transferase encoded by the nqr-associated apbe gene. analysis of complete bacterial genomes has revealed another putative gene (duf539, here renamed nqrm) that usually follows the apbe ge ... | 2016 | 26644436 |
| influence of oxyr on growth, biofilm formation, and mobility of vibrio parahaemolyticus. | vibrio parahaemolyticus is a common marine food-borne enteropathogen. in this study, we examined the antioxidative activity, growth, biofilm formation, and cell mobility of an oxyr deletion mutant and its genetically complementary strain of v. parahaemolyticus. oxyr is the regulator of catalase and ahpc genes. protection against extrinsic h2o2 and against the organic peroxides cumene hydroperoxide and tert-butyl hydroperoxide was weaker in the deletion mutant than in its parent strain. expressio ... | 2016 | 26590276 |
| sensitive visual detection of ahpnd bacteria using loop-mediated isothermal amplification combined with dna-functionalized gold nanoparticles as probes. | acute hepatopancreatic necrosis disease (ahpnd) is a component cause of early mortality syndrome (ems) of shrimp. in 2013, the causative agent was found to be unique isolates of vibrio parahaemolyticus (vpahpnd) that contained a 69 kbp plasmid (pap1) carrying binary pir-like toxin genes pirvpa and pirvpb. in thailand, ahpnd was first recognized in 2012, prior to knowledge of the causative agent, and it subsequently led to a precipitous drop in shrimp production. after vpahpnd was characterized, ... | 2016 | 27003504 |
| two endoplasmic reticulum proteins (calnexin and calreticulin) are involved in innate immunity in chinese mitten crab (eriocheir sinensis). | calnexin (cnx) and calreticulin (crt), which are important chaperones in the endoplasmic reticulum (er), participate in the folding and quality control of client proteins. cnx and crt identified from chinese mitten crab (eriocheir sinensis) are designated as escnx and escrt, respectively. escnx and escrt are expressed in the hemocyte, hepatopancrea, gill, and intestine at the mrna and protein level. immunofluorescence analysis indicated that escnx and escrt are located in the er. moreover, the m ... | 2016 | 27279413 |
| the brief case: safe to go back in the water? vibrio parahaemolyticus wound infection associated with brackish water. | 2016 | 27217385 | |
| a novel pcr-based approach for accurate identification of vibrio parahaemolyticus. | a pcr-based assay was developed for more accurate identification of vibrio parahaemolyticus through targeting the bla carb-17 like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. homologous analysis showed that bla carb-17 like genes were more conservative than the tlh, toxr and atpa genes, the genetic markers commonly used as detection targets in identification of v. parahaemolyticus. our data showed that this bla car ... | 2016 | 26858713 |
| development of a quadruplex loop-mediated isothermal amplification assay for field detection of four vibrio species associated with fish disease. | a quadruplex loop-mediated isothermal amplification (lamp) method was developed to detect four vibrio species, including vibrio ichthyoenteri, vibrio parahaemolyticus, vibrio scophthalmi, and vibrio vulnificus, simultaneously. four sets of species-specific primers were designed with different restriction sites contained in the inner primers. the quadruplex lamp method could distinguish four vibrio species via the subsequent restriction enzyme analysis. the sensitivity of the quadruplex lamp meth ... | 2016 | 27468405 |
| polar flagella rotation in vibrio parahaemolyticus confers resistance to bacteriophage infection. | bacteriophage has been recognized as a novel approach to treat bacterial infectious diseases. however, phage resistance may reduce the efficacy of phage therapy. here, we described a mechanism of bacterial resistance to phage infections. in gram-negative enteric pathogen vibrio parahaemolyticus, we found that polar flagella can reduce the phage infectivity. deletion of polar flagella, but not the lateral flagella, can dramatically promote the adsorption of phage to the bacteria and enhances the ... | 2016 | 27189325 |
| purification and host specificity of predatory halobacteriovorax isolates from seawater. | halobacteriovorax (formerly bacteriovorax) is a small predatory bacterium found in the marine environment and modulates bacterial pathogens in shellfish. four strains of halobacteriovorax originally isolated in vibrio parahaemolyticus o3:k6 host cells were separated from their prey by an enrichment-filtration-dilution technique for specificity testing in other bacteria. this technique was essential, since 0.45-μm filtration alone was unable to remove infectious vibrio minicells, as determined by ... | 2016 | 26590288 |
| molecular characteristics of lysozyme g in euryglossa orientalis; cdna cloning, phylogenic analysis, physicochemical properties and tissue gene expression. | lysozymes are the key molecules of innate immune system against bacterial infections. in the present study, we identified the molecular characteristics, physicochemical properties, antibacterial activity, evolutionary relationship and tissue expression pattern of g-type lysozyme in euryglossa orientalis (euor lysg). the full-length euor lysg cdna is composed of 588 nucleotides and an open reading frame encoding a protein with 195 amino acids with more than 65 % identity to g-type lysozyme of sol ... | 2016 | 27393386 |
| effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis). | the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ... | 2016 | 27208583 |
| antibacterial activity of litsea cubeba (lauraceae, may chang) and its effects on the biological response of common carp cyprinus carpio challenged with aeromonas hydrophila. | the aims of this study were to characterize the antibacterial activity and the chemotype of litsea cubeba leaf essential oil (eo) harvested in north vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (cyprinus carpio) challenged with aeromonas hydrophila. | 2016 | 27124660 |
| identification of polyvalent protective immunogens from outer membrane proteins in vibrio parahaemolyticus to protect fish against bacterial infection. | vaccination is one of the most effective and economic way to prevent infectious diseases in aquaculture. the development of effective vaccines, however, is still limited, especially for polyvalent vaccines, which are against multiple species. with this regard, identification of polyvalent protective immunogens, serving as polyvalent vaccines, became a key step in vaccine development. in the current study, 17 outer membrane proteins from vibrio parahaemolyticus were identified as immunogens. furt ... | 2016 | 27071519 |
| characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker larimichthys crocea. | galectins are a family of endogenous lectins with β-galactosides affinity, playing significant roles in the innate immunity of vertebrates and invertebrates. in this report, a new galectin-9 cdna was identified and characterized in large yellow croaker larimichthys crocea (designated as lcgal-9). the complete cdna sequence of lcgal-9 was 1795 bp, with an open reading frame (orf) of 1032 bp encoding 343 amino acids. the putative lcgal-9 protein contained two carbohydrate recognition domains (crds ... | 2016 | 26997199 |
| characterization of blooming algae and bloom-associated changes in the water quality parameters of traditional pokkali cum prawn fields along the south west coast of india. | in tropical shrimp farms, especially in traditional pokkali shrimp ponds, poor water quality management can lead to serious threats like heavy algal blooms and frequent disease outbreaks. an investigation regarding the frequent disease outbreaks in selected pokkali shrimp pond adjoining the cochin backwaters has been conducted. water quality parameters were analyzed during the study period. results indicate a considerable fluctuation in the concentration of dissolved oxygen (do) in the study in ... | 2016 | 26850711 |
| a novel c-type lectin, nattectin-like protein, with a wide range of bacterial agglutination activity in large yellow croaker larimichthys crocea. | c-type lectins (ctls) are generally recognized as a superfamily of ca(2+)-dependent carbohydrate-binding proteins, which serve as pattern recognition receptors (prrs) in innate immunity of vertebrates. in this study, the molecular characterization and immune roles of a novel ctl from larimichthys crocea (designated as lcntc) were investigated. lcntc is a novel protein that shared 33%-49% homology with other teleosts ctls. the full-length cdna of lcntc was composed of 859 bp with a 465 bp open re ... | 2016 | 26828263 |
| a novel c-type lectin is involved in the innate immunity of macrobrachium nipponense. | c-type lectins (ctls) play important roles in invertebrate innate immunity by recognizing and eliminating pathogens. in the present study, a low-density lipoprotein receptor class a (ldla) domain-containing ctl was identified from the oriental river prawn macrobrachium nipponense, designated as mnctldcp1. the full-length cdna of mnctldcp1 was composed of 1462 bp, with a 999-bp orf encoding a 332-aa protein. an ldla and a single c-type lectin-like domain (ctld) were found. the mrna transcripts of ... | 2016 | 26804648 |
| inhibition of pseudomonas aeruginosa exsa dna-binding activity by n-hydroxybenzimidazoles. | the pseudomonas aeruginosa type iii secretion system (t3ss) is a primary virulence determinant and a potential target for antivirulence drugs. one candidate target is exsa, a member of the arac family of dna-binding proteins required for expression of the t3ss. a previous study identified small molecules based on an n-hydroxybenzimidazole scaffold that inhibit the dna-binding activity of several arac proteins, including exsa. in this study, we further characterized a panel of n-hydroxybenzimidaz ... | 2016 | 26574012 |
| [monitoring and research on pathogen spectrum in patients with acute diarrhea from sentinel hospital of zhejiang province during 2009 to 2014]. | objective: to explore pathogen spectrum constitution of acute diarrhea in outpatient and emergency of zhejiang province, and provide basis for treatment, prevention and control of the disease. methods: during january 2009 to december 2014, we selected seven sentinel hospitals in different regions of zhejiang, monitored and researched on pathogen spectrum in patients with acute diarrhea from outpatient and emergency. we recorded patients' personal basic information, the main symptoms and signs, a ... | 2016 | 28057113 |
| establishment and characterization of a skin epidermal cell line from mud loach, misgurnus anguillicaudatus, (mase) and its interaction with three bacterial pathogens. | a continuous skin epidermal cell line from mud loach (misgurnus anguillicaudatus) (mase cell line) was established with its application in bacteria infection demonstrated in this study. primary mase cell culture was initiated at 26 °c in dulbecco's modified eagle medium/f12 medium (1:1; ph7.2) supplemented with 20% fetal bovine serum (fbs). the primary mase cells in spindle morphology proliferated into a confluent monolayer within 2 weeks, and were continuously subcultured even in 10% fbs- dmem/ ... | 2016 | 27288257 |
| antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago. | twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ... | 2016 | 28352539 |
| biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications. | in the present study, we report a green methodology for the synthesis of silver and gold nanoparticles, using the root extract of the herbal medicinal plant korean red ginseng. the silver and gold nanoparticles were synthesized within 1 h and 10 min respectively. the nanoparticles generated were not aggregated, and remained stable for a long time, which suggests the nature of nanoparticles. the phytochemicals and ginsenosides present in the root extract assist in reducing and stabilizing the syn ... | 2016 | 25706249 |
| a preliminary study on the antibacterial mechanism of tegillarca granosa hemoglobin by derived peptides and peroxidase activity. | the blood clam, tegillarca granosa, is one of the few bivalve molluscs containing hemoglobin (hb). in the present study, we purified two types of t. granosa hemoglobin, tg-hbi and tg-hbii, using size exclusion chromatography and measured their antibacterial and peroxidase activities. we also tested antibacterial activities of peptides prepared by trypsin digestion of purified tg-hb and reversed-phase high-performance liquid chromatography purification. purified tg-hbi and tg-hbii showed antibact ... | 2016 | 26876330 |
| the development of a green approach for the biosynthesis of silver and gold nanoparticles by using panax ginseng root extract, and their biological applications. | the biosynthesis of nanoparticles has received attention because of the development of economic and environmentally friendly technology for the synthesis of nanoparticles. the study develops a convenient method for the green synthesis of silver and gold nanoparticles by utilizing fresh root extract of the four-year old panax ginseng plant, and evaluated the antimicrobial applications of silver nanoparticles against pathogenic microorganisms. p. ginseng is a well-known herbal medicinal plant, and ... | 2016 | 25771716 |
| green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: siberian ginseng. | pharmacologically active stem of the oriental herbal adaptogen, siberian ginseng, was employed for the ecofriendly synthesis of siberian ginseng silver nanoparticles (sg-agnps) and siberian ginseng gold nanoparticles (sg-aunps). first, for metabolic characterization of the sample, liquid chromatography-tandem mass spectrometry analysis (indicated the presence of eleutherosides a and e), total phenol content, and total reducing sugar were analyzed. second, the water extract of the sample mediated ... | 2016 | 27468232 |
| extracellular synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 and their biological applications. | the present study highlights the microbial synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 strain, in an efficient way. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. the spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. the energy dispersive x-ray spectroscopy and elemental ... | 2016 | 26992796 |
| a strategic approach for rapid synthesis of gold and silver nanoparticles by panax ginseng leaves. | the study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of panax ginseng, an herbal medicinal plant. the reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°c, respectively. the developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. the nanopa ... | 2016 | 26698271 |
| characterization and antimicrobial application of biosynthesized gold and silver nanoparticles by using microbacterium resistens. | various microorganisms were found to be cable of synthesizing gold and silver nanoparticles when gold and silver salts were supplied in the reaction system. the main objective of this study was to evaluate the extracellular synthesis of gold and silver nanoparticles by the type strain microbacterium resistens(t) [kacc14505]. the biosynthesized gold and silver nanoparticles were characterized by ultraviolet-visible spectroscopy (uv-vis), field emission transmission electron micrograph (fe-tem), e ... | 2016 | 26597594 |
| weissella oryzae dc6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential. | nanoparticles and nanomaterials are at the prominent edge of the rapidly developing field of nanotechnology. recently, nanoparticle synthesis using biological resources has been found to be a new area with considerable prospects for development. biological systems are the masters of ambient condition chemistry and are able to synthesize nanoparticles by utilizing metal salts. in the perspective of the current initiative to develop green technologies for the synthesis of nanoparticles, microorgan ... | 2016 | 26212222 |
| a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces. | a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ... | 2016 | 26902211 |
| a specific antimicrobial protein cap-1 from pseudomonas sp. isolated from the jellyfish cyanea capillata. | a bacterium strain, designated as cmf-2, was isolated from the jellyfish cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. the strain cmf-2 was identified as pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16s rrna sequence analysis. in this study, an antimicrobial protein, named as cap-1, was isolated from the culture of cmf-2 through ammonium sulfate precipitation and gel filtration chromatography. ... | 2016 | 26529191 |
| recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis. | anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, w ... | 2016 | 27517939 |
| gfp tagged vibrio parahaemolyticus dahv2 infection and the protective effects of the probiotic bacillus licheniformis dahb1 on the growth, immune and antioxidant responses in pangasius hypophthalmus. | in this study, the pathogenicity of gfp tagged vibrio parahaemolyticus dahv2 and the protective effect of the probiotic strain, bacillus licheniformis dahb1 was studied on the asian catfish, pangasius hypophthalmus. the experiment was carried out for 24 days with three groups and one group served as the control (without treatment). in the first group, p. hypophthalmus was orally infected with 1 ml of gfp tagged v. parahaemolyticus dahv2 at two different doses (10(5) and 10(7) cfu ml(-1)). in the ... | 2016 | 26952171 |
| biosynthesis of silver nanoparticles using a probiotic bacillus licheniformis dahb1 and their antibiofilm activity and toxicity effects in ceriodaphnia cornuta. | in the present study, we synthesized and characterized a probiotic bacillus licheniformis cell free extract (blcfe) coated silver nanoparticles (blcfe-agnps). these blcfe-agnps were characterized by uv-visible spectrophotometer, xrd, edx, ftir, tem and afm. a strong surface plasmon resonance centered at 422 nm in uv-visible spectrum indicates the formation of agnps. the xrd spectrum of silver nanoparticles exhibited 2θ values corresponding to the silver nanocrystal. tem and afm showed the agnps ... | 2016 | 26802520 |
| complete genome of vibrio parahaemolyticus forc014 isolated from the toothfish. | foodborne illness can occur due to various pathogenic bacteria such as staphylococcus aureus, escherichia coli and vibrio parahaemolyticus, and can cause severe gastroenteritis symptoms. in this study, we completed the genome sequence of a foodborne pathogen v. parahaemolyticus forc_014, which was isolated from suspected contaminated toothfish from south korea. additionally, we extended our knowledge of genomic characteristics of the forc_014 strain through comparative analysis using the complet ... | 2016 | 27891183 |
| characterization of chemically-induced bacterial ghosts (bgs) using sodium hydroxide-induced vibrio parahaemolyticus ghosts (vpgs). | acellular bacterial ghosts (bgs) are empty non-living bacterial cell envelopes, commonly generated by controlled expression of the cloned lysis gene e of bacteriophage phix174. in this study, vibrio parahaemolyticus ghosts (vpgs) were generated by chemically-induced lysis and the method is based on minimum inhibitory concentration (mic) of sodium hydroxide (naoh), acetic acid, boric acid, citric acid, maleic acid, hydrochloric acid, and sulfuric acid. the mic values of the respective chemicals w ... | 2016 | 27854308 |
| evidences of abundant hemocyanin variants in shrimp litopenaeus vannamei. | hemocyanin (hmc) is a multifunctional immune molecule present in mollusks and arthropods and functions as an important antigen non-specific immune protein. our previous evidences demonstrated that litopenaeus vannamei hmc might display extensive molecular diversities. in this study, bioinformatics analysis showed dozens of variant sequences of the hmc subunit with higher molecular weight from l. vannamei (lvhmc). three variant fragments, named as lvhmcv1-3, which shared 85-99% nucleotide identit ... | 2016 | 27497189 |
| [gene deletion and functional analysis of the heptyl glycosyltransferase (waaf) gene in vibrio parahemolyticus o-antigen cluster]. | to construct heptyl glycosyltransferase gene ii (waaf) gene deletion mutant of vibrio parahaemolyticus, and explore the function of the waaf gene in vibrio parahaemolyticus. | 2016 | 27373077 |
| prevalence and antibiotic resistance of bacterial pathogens isolated from childhood diarrhea in beijing, china (2010-2014). | diarrhea is one of the main causes of morbidity and mortality among children less than 5 years of age worldwide, and its causes vary by region. this study aimed to determine the etiologic spectrum, prevalent characteristics and antimicrobial resistance patterns of common enteropathogenic bacteria from diarrheagenic children in beijing, the capital of china. | 2016 | 27303446 |