Publications

the two-component qsebc signalling system regulates in vitro and in vivo virulence of aeromonas hydrophila.	we recently demonstrated that the n-acyl-homoserine lactone [autoinducer (ai)-1] and luxs (ai-2)-based quorum-sensing (qs) systems exerted positive and negative regulation, respectively, on the virulence of a diarrhoeal isolate ssu of aeromonas hydrophila. however, the role of a newly identified, two-component-based qsebc qs system in the regulation of bacterial virulence in general is not well understood, with only a limited number of studies showing its function in bacterial pathogenesis. in t ...	2012	21998161
The spoilage of air-packaged broiler meat during storage at normal and fluctuating storage temperatures.	Bacterial diversity and the major flora present on air-packaged broiler meat during storage at normal (4°C) and fluctuating storage temperatures (0-4°C and 4-10°C) were investigated using culture-dependent and culture-independent approaches. Culture-dependent analysis revealed that the growth of microflora was retarded when broiler meat was stored at lower temperatures (0-4°C). Denaturing gradient gel electrophoresis profiles showed that Staphylococcus spp., Pseudomonas spp., Acinetobacter spp., ...	2012	22184446
Bacterial infections of Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to gamete collecting weirs in Michigan.	Herein, we describe the prevalence of bacterial infections in Chinook salmon, Oncorhynchus tshawytscha (Walbaum), returning to spawn in two tributaries within the Lake Michigan watershed. Ten bacterial genera, including Renibacterium, Aeromonas, Carnobacterium, Serratia, Proteus, Pseudomonas, Hafnia, Salmonella, Shewanella and Morganella, were detected in the kidneys of Chinook salmon (n = 480) using culture, serological and molecular analyses. Among these, Aeromonas salmonicida was detected at ...	2012	22168454
evaluation of a quantitative h(2)s mpn test for fecal microbes analysis of water using biochemical and molecular identification.	the sensitivity and specificity of the h(2)s test to detect fecal bacteria in water has been variable and uncertain in previous studies, partly due to its presence-absence results. furthermore, in groundwater samples false-positive results have been reported, with h(2)s-positive samples containing no fecal coliforms or escherichia coli. false-negative results also have been reported in other studies, with h(2)s-negative samples found to contain e. coli. using biochemical and molecular methods an ...	2012	22244995
identification and molecular characterization of the interleukin-10 receptor 1 of the zebrafish (danio rerio) and the goldfish (carassius auratus l.).	this is the first report of the identification and molecular characterization of an interleukin-10 receptor 1 in bony fish. by gene synteny analysis, we identified the zebrafish interleukin-10 receptor 1 (il10r1) and using this il10r1 sequence, we cloned the goldfish il10r1 cdna transcript. the identified fish il10r1 protein sequences had a putative jak1 binding site, only one of the two stat3 binding sites, that are present in all other vertebrates il10r1 proteins as well as c-terminal serine r ...	2012	21906622
Bdellovibrios, potential biocontrol bacteria against pathogenic Aeromonas hydrophila.	Recent studies have revealed that the use of bdellovibrios is an alternative to control bacteriosis. However, no bdellovibrios are available against Aeromonas hydrophila infections in sturgeons. In the present study, a potential Bdellovibrio strain F16 was isolated from sturgeon gut samples, using a sturgeon-pathogenic A. hydrophila as the prey bacterium. It was initially identified as a Bdellovibrio strain using morphological characteristics and specific PCR amplification, and confirmed to be B ...	2012	21864996
The Aeromonas dsbA mutation decreased their virulence by triggering type III secretion system but not flagella production.	Pathogenesis of Aeromonas species have been reported to be associated with virulence factors such as lipopolysaccharides (LPS), bacterial toxins, bacterial secretion systems, flagella, and other surface molecules. Dsb (Disulfide bond) proteins play an important role in catalyzing disulfide bond formation in proteins within the periplasmic space. An A. hydrophila dsbA mutant with attenuated virulence using Dictyostelium amoebae as an alternative host model was identified. The attenuated virulence ...	2012	22198000
Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture.	Aims: Subcellullar localizations and cross-immunities of GAPDHs from six common pathogenic bacteria in aquaculture were investigated. Methods and Results: Subcellullar localizations of GAPDHs of Edwardsiella tarda EIB202, Edwardsiella ictaluri ATCC33202, Aeromonas hydrophila LSA34, Vibrio anguillarum MVM425, Vibrio alginolyticus EPGS020401 and Vibrio harveyi VIB647 were analysed with Western blotting, indirect immunofluorescence and flow cytometry examinations. Immunoprotections of different r ...	2012	22017642
identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis candidates.	to describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ...	2012	22074685
a small incq type plasmid carrying the quinolone resistance (qnrs2) gene from aeromonas hydrophila.	aims: to investigate the qnrs2 gene encoded by a plasmid obtained from aeromonas hydrophila. methods and results: to investigate the full length sequence of the plasmid carrying qnrs2 (plasmid designated pahh04) from the strain snufpc-a10, the full length coding sequence of the qnrs region was first amplified. the remaining part of the plasmid was read outwards from this region. the plasmid pahh04 contained the repc, repa, moba and mobc genes and its total size was 7,191 bp with a g + c conten ...	2012	22260532
effects of graded levels of dietary methionine hydroxy analogue on immune response and antioxidant status of immune organs in juvenile jian carp (cyprinus carpio var. jian).	immune response and antioxidant status of immune organs in juvenile jian carp (cyprinus carpio var. jian) fed graded levels of methionine hydroxy analogue (mha) (0, 5.1, 7.6, 10.2, 12.7, 15.3 g kg(-1) diet) for 60 days were investigated. results indicated that head kidney index, spleen index, red and white blood cell counts significantly increased with increasing mha levels up to a point (p < 0.05), whereupon decreased (p < 0.05). glutathione reductase activity in head kidney and spleen, anti-hy ...	2012	22245840
an aerolysin-like enterotoxin from vibrio splendidus may be involved in intestinal tract damage and mortalities in turbot, scophthalmus maximus (l.), and cod, gadus morhua l., larvae.	vibrio splendidus is a pathogen that can cause major losses during the early stages of larval turbot rearing when live feed (rotifers or artemia) is used. as haemolytic bacteria have often been associated with larval rearing losses, we studied the role of the v. splendidus haemolysin in infection of larvae. from a bank of over 10 000 transposon mutants of v. splendidus, two different types of haemolysin-negative mutants were obtained. both had lost virulence for larval fish, and immunohistochemi ...	2012	22233514
prevalence and antimicrobial susceptibilities of vibrio, salmonella, and aeromonas isolates from various uncooked seafoods in thailand.	uncooked seafood samples were collected from open markets and supermarkets in bangkok, thailand, and were examined for the presence of vibrio, salmonella, and aeromonas species from january to february 2008. from 120 samples, 272 bacterial isolates were identified through biochemical testing. of all sea bass, shrimp, oyster, and blood cockle samples (30 of each) that were processed for culture, 114 (95%) samples had at least one detectable isolate of vibrio, salmonella, or aeromonas, leaving on ...	2012	22221354
baltic salmon, salmo salar, from swedish river lule älv is more resistant to furunculosis compared to rainbow trout.	furunculosis, caused by aeromonas salmonicida, continues to be a major health problem for the growing salmonid aquaculture. despite effective vaccination programs regular outbreaks occur at the fish farms calling for repeated antibiotic treatment. we hypothesized that a difference in natural susceptibility to this disease might exist between baltic salmon and the widely used rainbow trout.	2012	22276121
Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products.	Aims: Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae (phaC(Ac) ) was transformed into this strain. Methods and Results: The resulting transformant incorporated approximately 1 ± 0·3 mol% of 3HHx ...	2012	22054430
the patient presenting with acute dysentery - a systematic review.	objectives: the etiologies, clinical presentations and diagnosis of acute pathogen-specific dysentery in children and adults in industrialized and developing regions is described to help develop recommendations for therapy. methods: we conducted a systematic review of literature published between january 2000 and june 2011 to determine the frequency of occurrence of pathogen-specific dysentery. results: shigella, salmonella, and campylobacter remain the most frequent bacterial causes of dysenter ...	2012	22266388
immunomodulatory effect of withania somnifera supplementation diet in the giant freshwater prawn macrobrachium rosenbergii (de man) against aeromonas hydrophila.	the effect of withania somnifera extract supplementation diets on innate immune response in giant freshwater prawn macrobrachium rosenbergii (de man) against aeromonas hydrophila was investigated. the bacterial clearance efficiency significantly increased in prawn fed with 0.1% and 1.0% doses of w. somnifera supplementation diet against pathogen from weeks 1-4 as compared to the control. the innate immune parameters such as, phenoloxidase activity, superoxide anion level, superoxide dismutase ac ...	2012	22118967
In vitro evaluation of antibacterial activity of Asparagopsis taxiformis from the Straits of Messina against pathogens relevant in aquaculture.	Ethanol extracts of Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antibacterial activity against pathogenic shellfish and fish bacteria previously isolated from local marine and brackish environments. Genetic labelling by DNA barcoding allowed us to identify the algal population as a biogeographical strain conspecific to A. taxiformis. The extract obtained in May showed the broadest antibacterial activity against all tested pathogenic bacteria, especiall ...	2012	22078751
Phenoloxidase in the scallop Chlamys farreri: Purification and antibacterial activity of its reaction products generated in vitro.	Phenoloxidase (PO) was purified from hemocytes of the scallop Chlamys farreri using native-PAGE and gel permeation column chromatography, and then substrate specificity and antibacterial activity generated from reaction products of purified PO were analyzed. The results showed purified PO had a molecular mass of 576 kDa in native-PAGE and 53 kDa in denatured PAGE, and could catalyze the substrates L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, catechol and hydroquinone suggesting it is a type ...	2012	22119576