Publications

use of tergitol-7 agar in the clinical laboratory.	tergitol-7 agar has been utilized in the author's laboratory for eight years and has been found the superior medium for the rapid isolation, separation and identification of gram negative bacilli found in a clinical setting.	1975	803348
[the relationship between the structure and antimicrobial activity of several substituted omega-nitrosterols].		1975	803457
demonstration of cell division by septation in a variety of gram-negative rods.	through use of an initial fixative employing a combination of crotonaldehyde and glutaraldehyde, septa were preserved in thin sections of dividing cells of strains of pseudomonas aeruginosa, salmonella typhimurium, shigella sonnei, and escherichia coli when grown at 30 c in a dilute basal medium. the same procedures, however, revealed only a constrictive division process in proteus vulgaris and erwinia sp. this adds to the evidence that septation, although difficult to demonstrate, is the proces ...	1975	803487
the role of vaginal colonization with enterobacteriaceae in recurrent urinary infections.	quantitative cultures of the vaginal introitus for enterobacteriaceae, s. faecalis and p. aeruginosa were compared in 20 premenopausal normal women (200 cultures) to 198 consecutive cultures from 9 premenopausal women in between episodes of bacteriuria. introital colonization in patients susceptible to urinary infections was significantly higher for e. coli (p equals 0.001), s. faecalis (p equals 0.001) and for the presence of any gram-negative pathogens (p equals less than 0.001). in addition, ...	1975	803573
chondroitinase-producing bacteria in natural habitats.	a search was undertaken for bacteria which degrade chondroitin sulfate in nature and to find bacteria with a usefully high rate of chondroitinase (chsase) productivity. first, 253 chsase-producing bacteria were obtained from aquatic and land environments in japan by aerobic and anaerobic screening methods. identification according to bergey's manual of determinative bacteriology or bain and shewan (1968) permitted assignment of the majority of the isolates to seven genera, aeromonas, vibrio, fla ...	1975	803822
immunological studies of an antigen common to many gram-negative bacteria with special reference to e. coli. characterization and biological significance.	a number of different bacterial strains were analyzed for the presence of common antigens using immunodiffusion techniques. most of the studied e. coli strains had many common antigens. especially one antigen was found in all investigated e. coli as well as proteus and pseudomonas strains and antibodies to this antigen were also recognized in antimeningococcal antiserum. the antigen was found possibly to be at least partly a protein with low carbohydrate content located in the inner part of the ...	1975	803931
computer surveillance of shifts in the gross patient flora during hospitalization.	data accumulating routinely in a hospital microbiology laboratory were computer-plotted according to the number of days the patient had been in the hospital when each culture was obtained. the rate at which patients were cultured fell slightly during hospitalization, while the rate at which isolates were obtained from them increased gradually. for some species, such as escherichia coli or staphylococcus aureus, isolation rates changed little during hospitalization. they rose as much as sevenfold ...	1975	804021
rapid detection and identification of enteric bacteria from blood cultures.	the bactec radiometric system and inolex enteric i card were used in conjunction for the rapid detection and presumptive identification of enteric gram-negative rods in blood cultures. excellent correlation was obtained between the inolex card and routine identification techniques when organisms from both artificially inoculated and clinical blood cultures were studied. in many instances, the culture report was available less than 24 hr after receipt of the specimen.	1975	804025
[seasonal aspects of hospital infection in a urological hospital].		1975	804198
comparison of amikacin and gentamicin.	the sensitivity to gentamicin (g) and to amikacin (a) of 25 strains of escherichia coli (ec), 25 klebsiella sp. (k), 25 proteus and providence sp. (pp), and 25 ps. aeruginosa (pa) were tested in vitro by the disc method, by the inocula-replicating method, and by a tube dilution technique using 10-5 microorganisms/ml. bactericidal concentrations active on 50% and 90% of the strains were 1.5 and 4.5 mug/ml for ec, 3 and mug/ml for k, 1.5 and 3 mug/ml for pp, and 3 and 3 mug/ml for pa. resistance t ...	1975	804370
herd studies on coliform mastitis.	escherichia coli, enterobacter aerogenes, and klebsiella pneumoniae were responsible for 63, 10, and 11 percent, respectively, of 158 coliform organisms recovered from mastitis cases in 8 california herds. the severity and nature of the coliform mastitis problem were found to vary greatly among herds but were characteristic for each of 4 herds studied in detail.	1975	804464
a membrane filter technique for testing disinfectants.	a membrane filter was used for assessing the surface disinfecting activity of phenolic disinfectants and a chloroxylenol disinfectant. the influence of the type of organism, inoculum size, and hardness of water was investigated. pseudomonas aeruginosa was chosen for the standardized test. disinfectant solutions were prepared in water of 300 ppm hardness and applied for two and a half minutes and eight minutes to the bacteria deposited from filtration of 1 ml of a suspension containing 10-6 bacte ...	1975	804497
[allergenic fractions of enterobacteriaceae. vi. gel-chromatographic profile of intracellular protein antigens, forming the allergen-active substrate of enterobacteria].	allergeno-active protein substrate of enterobacteria (e. coli and r. rettgeri strains) was divided into 7 fractions with a different molecular weight with the aid of many-stage gel-filtration through sephadex (g-50, g-75, g-100, g-150, g-200). a study was made of their biochemical composition, antigenic and protein spectrum, allergenic properties in the reactions of hypersensitivity of delayed type in vivo (skin tests) and in vitro (inhibition of macrophage migration). the principal part of alle ...	1975	804783
qualitative assessment of midstream urine cultures in the detection of bacteriuria.	3550 women attending cervical smear and family planning clinics have been screened for bacteriria. suprapubic aspiration of urine was carried out in 131 patients from whom two consecutive midstream urine specimens contained either a pure growth of gram positive cocci or a growth in which gram negative bacilli predominated. 95 patients were found to have bacteriuria; in 93 the urine was infected with a gram negative organism. of 53 patients with a count of more than 10-5 gram negative organisms p ...	1975	805012
treatment of urinary tract infections unresponsive to sulfa with carbenicillin indanyl sodium.		1975	805021
agglutinin response to bacterial infection in acute exacerbations of chronic bronchitis.	agglutinin titres to haemophilus influenzae, streptococcus pneumoniae, klebsiella pneumoniae, staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, and proteus vulgaris in the serum of patients with acute exacerbations of chronic bronchitis, patients producing mucoid sputum, and healthy controls were determined. serological evidence of infection with h. influenzae was found in 38 of 57 patients with acute exacerbations, and str. pneumoniae infection in 10 of the 57 patients, but was g ...	1975	805163
effectiveness of achievable urinary concentrations of tetracyclines against "tetracycline-resistant" pathogenic bacteria.	hospitalized patients with urinary tract infections caused by pseudomonas aeruginosa or other bacterial pathogens are frequently treated with parenteral antibiotics such as gentamicin. many of these organisms are shown by kirby-bauer disk sensitivity testing to be resistant to tetracycline. one hundred seventy-one such tetracycline-resistant bacterial isolates were studied; 84% were found to be sensitive to achievable urinary concentrations of tetracycline. two patients with long-standing chroni ...	1975	805188
in-vitro effect of edta-tris-lysozyme solutions on selected pathogenic bacteria.	the in-vitro effect of edta-tris-lysozyme solution on 16 pathogenic bacteria of medical or veterinary importance was determined. marked decreases in bacterial count occurred with pseudomonas aeruginosa, escherichia coli, moraxella osloensis and campylobacter fetus, and smaller decreses with salmonella typhimurium, shigella boydii, aeromonas hydrophila, proteus mirabilis, listeria monocytogenes and erysipelothrix insidiosa. the test solution had no effect on klebsiella ozaenae, brucella canis, co ...	1975	805241
urinary tract infection treated with oxolinic acid.		1975	805351
comparison of the germicidal activity of prepodyne and betadine surgical scrub solutions.	two iodophoric surgical scrubs soaps, prepodyne and betadine, were evaluated for bacteriocidal activity in a modified phenol coefficient test. a standard inoculum of each of 18 species of microorganisms was mixed with a 1:8 dilution of each iodophor and subcultured after one, three, and five minutes of exposure. complete cidal activty was exhibited by both prepodyne and betadine. twenty-five subjects were tested in hand washing experiments using betadine and prepodyne surgical scrub solutions. q ...	1975	805538
probability graphs resolve components with buoyant-density differences of 0.001 g/cm3 in equilibrium density gradients.		1975	805551
asymptomatic bacteriuria in schoolchildren in newcastle upon tyne.	a screening survey for asymptomatic bacteriuria (asb) in 13464 schoolgirls aged 4 to 18 years in newcastle upon tyne showed an overall prevalence of 1.9%. in girls 4 to 6 years it was 1.4%, in girls aged 7 to 11 years it was 2.5%, in girls aged 12 to 18 years it was 1.6%, a statistically significant rise and fall. renal scarring was found in 39 (15%) of 254 girls with asb. neither the prevalence nor the severity of renal scarring increased with age. there was no association between asb and socia ...	1975	805569
hydrogen peroxide sterilization of hydrophilic contact lenses.	a new, simple, and effective procedure for the sterilization of soft contact lenses utilizes hydrogen peroxide, an inexpensive and readily availiable solution.	1975	805580
volunteer and clinical studies with carfecillin: a new orally administered ester of carbenicillin.	blood and urine levels of carbenicillin were measured in 10 healthy volunteers and four patients with renal failure after single and multiple oral dose of carfecillin. urinary levels after 1000-mg doses in healthy subjects were considered sufficient for treatment of pseudomonas aeruginosa urinary infections, but the serum levels were too low for chemotherapy of systemic infections with this organism even in severe renal failure. urinary infections were treated in 35 inpatients with a seven-day c ...	1975	805625
the fate and origin of the nuclear envelope during and after mitosis in amoeba proteus. i. synthesis and behavior of phospholipids of the nuclear envelope during the cell life cycle.	the synthesis and behavior of amoeba proteus nuclear envelope (ne) phospholipids were studied. most ne phospholipid synthesis occurs during g2 and little during mitosis or s. (a. proteus has no g1 phase). autoradiographic observations after implantation of [3-h] choline nuclei into unlabeled cells reveal little turnover of ne phospholipid during interphase but during mitosis all the label is dispersed through the cytoplasm. beginning at telophase all the label is dispersed through the cytoplasm. ...	1975	805790
[nature of bacteriocins].	the bacteriocins comprise a large, heterogeneous group of bactericidal substances produced by bacteria from widely differing species. the best known bacteriocins are the colicins. some aspects of research on colicins are discussed especially their chemical nature and mode of action, and their genetic determinants.	1975	806026
pseudomonas aeruginosa r factors determining gentamicin plus carbenicillin resistance from patients with urinary tract colonizations.	r factors determining multiple resistance including both gentamicin and carbenicillin have been identified in high incidence among hospital isolates of pseudomonas aeruginosa. the factors are readily transmitted to other p. aeruginosa but not to escherichia coli strains k-12 or c, or to proteus mirabilis. r factor-containing isolates are predominantly immunotype 7 isolated from urinary sources.	1975	806258
function of the classical and alternate pathways of human complement in serum treated with ethylene glycol tetraacetic acid and mgcl2-ethylene glycol tetraacetic acid.	an immunochemical and functional analysis of the classical and alternate complement pathways in human serum was performed in the presence of 10 mm ethylene glycol tetraacetic acid (egta) and mgcl(2)-egta (mgegta), chelating agents which have been recently utilized as a means of distinguishing between these two complement pathways. total hemolytic activity, integrity of the c1 complex, hemolytic activity of c2, conversion of factor b (c3 proactivator), and complement-dependent bactericidal activi ...	1975	806523
characterization of a non-type-specific antigen(s) associated with group a streptococcal type 12 m protein.	the sharing of one and possibly two or more non-type-specific antigens by most strains of groups a, c, and g streptococci is described. with the exception of a single strain of proteus mirabilis, this antigen(s) was not found among strains of groups b and d streptococci, coagulase-positive staphylococci, escherichia coli, pseudomonas sp., and salmonella sp. the non-type-specific antigen(s) could not be separated from m protein by fractionation with various saturations of ammonium sulfate or by c ...	1975	806525
[clinical study with spectacillin on patients with infections of the urogenital system (author's transl)].	report is given of clinical experiences gained with the new developed semi-syntheticpenicillin "spectacillin" on 75 patients suffering from an urological disease. the drug has been applied for therapy of acute and chronic forms of inflammations of the bladder and the urethra, additionally the prostatitis, the epididymitis, but first of all the pyelonephritis (caused bacterially). within the germ spectrum of these diseases, germs of the coli-type, streptococcus faecalis (enterococcus), staphyloco ...	1975	806772
[ciclacillin therapy for diseases of the urinary tract].	in our own controlled bacteriological investigations, ciclacillin showed a good effect in urological infections at a daily dosage of 3 g for 10 days. the efficacy is comparable to that of ampicillin and corresponds to that of the double blind trials bescribed in the literature. the specially good tolerance is worthy of note.	1975	806791
the varying sensitivity to antibacterial agents of micro-organisms in pure vs. mixed cultures.	in this study the disc sensitivities of five organisms growing in pure cultures (staphylococcus aureus, beta-hemolytic streptococcus, proteus mirabilis, pseudomonas aeruginosa, and candida albicans) were first determined against each of seven antibacterial agents (penicillin, streptomycin, gentamicin, kanamycin, silver nitrate, sulfamylon, and betadine). then the sensitivity of each organism growing in combination with one of the others (10 combinations) was tested against each of the same antib ...	1975	806983
[significance of the antibacterial agent assay of urine for bacteriological diagnosis and control of chemotherapy of urinary tract infections (author's transl)].	the disc agar-diffusion-test using bacillus subtilis atcc 6051 as test organism is a simple and rapid method for routine testing of antibacterial agents in urine specimens. the test records urine levels which are expected under medium dosage, and in many cases even lower concentrations of renal excreted antibiotics. out of 5655 analysed urine samples 22% contain antibacterial substances. in urine specimens over which information was volunteered that either no chemotherapy had been administered o ...	1975	807057
a critical look at chemical disinfection of anaesthetic apparatus.	studies were carried out to test the efficacy of disinfection of a previously contaminated corrugated anaesthetic tube and reservoir bag. bacteriological swabs and sampling with broth were evaluated as methods of detecting contamination. gross contamination was detected by broth sampling on occasions when swabbing failed to reveal contamination. the presence of up to 18% by volume of residual air within the apparatus was detected. a method of ensuring elimination of all air is described and stud ...	1975	807229
comparative activity of tobramycin and gentamicin against pseudomonas, proteus and providencia species.	tobramycin is a new antibiotic resembling gentamicin. we measured the minimal inhibitory concentrations of these two antibiotics against five bacterial species that cause hospital-acquired infections and are resistant to many presently available antibiotics. the organisms tested were 500 strains of pseudomones aeruginosa, 100 strains of each of proteus rettgeri and pr. morganii, 50 strains of pr. vulgaris and 250 strains of providencia stuartii. tobramycin was 2 to 4 times more active than genta ...	1975	807310
comparison of the in vitro activities of bb-k8 and three other aminoglycosides against 215 strains of pseudomonas and enterobacteriaceae with variable sensitivity to kanamycin and gentamicin.	215 gram-negative bacilli isolated from clinical materials were tested in vitro against bb-k8 by means of disc diffusion and agar dilution tests; the strains included 40 isolates resistant to gentamicin. approximately 90% of the strains were inhibited by 3.12 mug/ml or less bb-k8. this antibiotic exhibited a comparable activity, although somewhat inferior, to that of gentamicin, against organisms sensitive to gentamicin. it was considrably more active than gentamicin, and comparable to tobramyci ...	1975	807457
systematic difference in the methylation of ribosomal ribonucleic acid from gram-positive and gram-negative bacteria.	a survey of gram-positive and gram-negative organisms was performed to compare the distributionof n6-methylated adenine. it was found that (i) all the gram-positive strains tested, staphylococcus aureus, sarcina lutea, bacillus stearothermophilus, bacillus subtilis, and bacillus megaterium, contain neither n6-monomethyl adenine (m6a) nor n6-dimethyladenine (m26a) in 23s ribosomal ribonucleic acid (rrna). in the case of s. aureus and streptococcus pyogenes, strains which are clinically resistant ...	1975	807565
detection of endotoxins with the limulus test in burned and unburned mice infected with different species of gram-negative bacteria.	the limulus test detected endotoxins in the plasma of burned and unburned mice infected with different species of gram-negative bacteria produced different amounts of endotoxin in the plasma of infected mice. plasma from mice given lethal infections showed very high concentrations of endotoxin. low concentrations of endotoxin in the plasma were tolerated by mice but high concentrations were invariably fatal. a polyvalent pseudomonas vaccine reduced endotoxin in the plasma of mice given lethal in ...	1975	807621
interference with alpha-antitrypsin studues in stored serum by presumed bacterial proteases.	contamination of werum by certain gram-negative bacteria has been shown to spoil the serum for measurement of trypsin inhibitory capacity (stic) or for antitrypsin phenotyping. such sera develop intense fibrinolytic activity when the stic has dropped to itsminimal level, but antitrypsin concentration as measured by radial immunodiffusion remainsconstant. cultures of enterobacter, klebsiella, bacillus subtilis, and pseudomonas species were shown to have this capability, but production of the fi ...	1975	807660
the use of analytical isoelectric focusing for detection and identification of beta-lactamases.	beta-lactamases (ec. 3.5.2.6) from strains of gram-negative bacteria have been studied using analytical isoelectric focusing. this permits a visual comparison of the patterns of beta-lactamase bands produced by enzymes from different organisms. purification of crude intracellular preparations is unnecessary and the technique is sufficiently sensitive to demonstrate beta-lactamase in mutants previously reported to lack the enzyme. r that have not been distinguished from one another biochemically ...	1975	807678
contractility of glycerinated amoeba proteus and chaos-chaos.	immediate contact with large volumes of cold 50% (v/v) buffered glycerol preserved typical ameboid shape of chaos chaos and amoeba proteus with no visible distortions. these technics allowed determination of the contraction sites in these glycerinated models upon applications of atp-ca-mg-solutions. the ectoplasmic tube was the main site of contraction. preliminary em investigations revealed thick and thin filaments, associated with the ectoplasmic tube near the plasma-lemma, which appeared to b ...	1975	807722
substituted 1-[(5-nitrofurfurylidene)amino]-4-imidazolin-2-ones.	a series of 1-[(5-nitrofurfurylidene)amino]-4-imidazolin-2-ones has been prepared. a new synthesis of 4-alkyl-1-[(5-nitrofurfurylidene)amino]-4-imidazolin-2-ones involving the oxidative ring closure of 5-nitro-2-furaldehyde 2-(2-hydroxyethylalkyl)semicarbazones is described. the in vitro testing of the compounds against a variety of bacteria is reported.	1975	807733
[amikacin treatment of septicemia due to gentamicin-resistant gram-negative bacilli (author's transl)].		1975	807757
[bacteriological studies on fosfomycin. antibacterial activity in vitro and in vivo (author's transl)].	we have examined the antibacterial activity of the new antibiotic, fosfomycin (fom) in vitro and in vivo. the following results were obtained. 1. fom showed a broad antibacterial spectrum. 2. the antibacterial activity of fom was enhanced in the medium at ph 6 and ph 7, and was also influenced by the addition of rabbit serum, calf serum, glucose-6-phosphate or defibrilated sheep blood to the growth medium, and by the size of inoculum. 3 fom showed especially strong bactericidal action upon the b ...	1975	807758
[laboratory studies and therapeutic effect on respiratory infections of a combined preparation of ampicillin and cloxacillin (author's transl)].		1975	807759
competitive effects of intestinal microflora on vibrio cholerae in gnotobiotic mice.	the coexistence of vibrio cholerae and several intestinal bacteria was determined in gnotobiotic mice. the bacteria tested included a bacteroides sp, clostridium difficile, clostridium perfringens type a, 2 separate isolates of escherichia coli, 2 different lactobacilli, 2 separate isolates of proteus mirabilis, pseudomonas maltophilia, and streptococcus faecalis. each species of bacteria became established and was recovered repeatedly from the stools during the studies. no single strain or spec ...	1975	807775
[hospital infections from the neurosurgical point of view (author's transl)].	a report is made on infections of the trachea and of the bladder which occurred in patients of a neurosurgical intensive care unit. the development of resistance and counter-measures are discussed in detail.	1975	807839
otitis externa in the dog -- a clinical and microbiological study.	the effect on canine otitis externa of a topical preparation composed of fucidin, framycetin, nystatin, and prednisolone was evaluated in a clinical trial comprising 235 dog ears. cases caused by parasites or foreign bodies and cases requiring systemic therapy were excluded. swabs from these and an additional 434 affected ears were examined bacteriologically and the sensitivity to antibiotics of the bacteria isolated determined. the infection most frequently diagnosed was staphylococci plus yeas ...	1975	807907
relationship of bacterial infection in urine and calculi to canine urolithiasis.	in 26 dogs treated surgically for urolithiasis, bacteriological examination of the urine and the interior of calculi showed that infection was present in both materials in 14 cases. infection with phosphate calculi, present in 13 of these 14 dogs, was associated with a variety of bacteria including staphylococcus aureus, staph epidermidis, streptococcus faecalis, escherichia coli and proteus spp. in a follow-up examination of 16 dogs, organisms different from the original isolates were recovered ...	1975	808018
nation-wide survey of antibiotic resistance by means of a computer. analysis of 200.000 strains of problem bacteria isolated in 1973.	based on a pilot study performed in 5 district public health laboratories (phl) in the slovakia in 1972 (1), the computer system for elaborating the informations concerning the resistance of "problem bacteria" to antibiotics has completely been introduced in 18 phls for all-year studies on the resistance to antibiotics in 1973. the results obtained in each phl and also in total of about 200.000 bacterial strains belonging to 8 of the clinically most important bacterial species were passed on to ...	1975	808050
the prophylactic use of keflex and keflin in vaginal hysterectomy.	although the number of severe infections occurring after vaginal hysterectomy are few,they sometimes result in the death of a patient or a protracted hospital course. a prospective double-blind study, using keflex and keflin in theraputic doses,was undertaken to elucidate more clearly the effect upon morbidity in vaginal hysterectomy. cultures were taken form a catheterized urine specimen and the cervix of all patients before surgery. cultures were repeated on the fourth postoperative day. mo ...	1975	808126
arylamidase activity of salmonella species.	arylamidase activity in cell extracts of sonically cell treated suspensions of 23 salmonella strains, including 12 strains of s. typhimurium, was investigated. all cultures hydrolyzed five of nine different neutral and basic substrates. activity against aspartyl-, cystyl- histidinyl-, and isoleucyl-beta-naphylamide was negligible. alanyl-beta-naphthylamide was the preferred substrate for the salmonella species; however, specific activities ranged widely. of several gram-negative organisms survey ...	1975	808165
[comparison of the esterases of providencia alcalifadiens and providencia stuartii].		1975	808353
carbenicillin therapy of urinary tract infections due to difficult uropathogens. i. indole-positive proteus.		1975	808383
carbenicillin therapy of urinary tract infections dur to difficult urop athogens. iv. summary and conclusions.		1975	808386
laboratory studies with cefatrizine (sk + f 60771), a new broad-spectrum orally-active cephalosporin.	cefatrizine (sk&f 60771), a new orally-active semisynthetic cephalosporin antibiotic with broad-spectrum antibacterial activity, was compared with cephalexin and cefazolin for in vitro and in vivo antibacterial activity and pharmacokinetic behavior in laboratory animals. the average mic values obtained with cefatrizine against gram-positive and gram-negative bacteria were superior to those obtained with cephalexin and somewhat poorer than those of cefazolin. in addition, a large percentage of th ...	1975	808525
aromatic esters of 5-o-desosaminylerythronolide a oxime.	several substituted aromatic esters of the c-3 hydroxyl of 5-o-desosaminylerythronolide a oxime were prepared. ribosomal binding studies showed that meta substituents on the aromatic ring gave the most active analogs. the esters described were all inactive in vivo at the maximum level tested.	1975	808612
[the fundamental and clinical studies on pt-122m (doxycycline) in the otorhinolaryngological field (author's transl)].	as the results of laboratory and clinical investigation with a new tetracycline derivate, pt-122m (doxycycline) was performed with the results which may lead to the following conclusions. 1) in vitro antibacterial activity: the minimal inhibitory concentration of doxycycline (dotc) was measured by an agar plate dilution method. the mic of dotc against 60 strains of coagulase-positive staphylococcus aureus isolated from otorrhoea was distributed over a range of 0.2 approximately 1.56 mcg/ml and 1 ...	1975	808657
a review of 105 necropsies in captive baboons (papio cynocephalus).	in contrast to the findings in newly caught babboons in africa, the leading cause of death in captivity in america was a pneumonia and enteritis complex. bacterial species such as proteus morgani, proteus mirabilis, clostridium sp., pseudomonas paracolon, pathogenic escherichia coli and cocci have been isolated in association with a pneumoenteric syndrome of 35 newborn baboons, including neonates. the majority of these animals died with pulmonary factors such as anoxia and pneumonia.	1975	808667
[bacteriological detection of asymptomatic urinary tract infections].		1975	808673
detection of bacteriuria in pediatric outpatients. a new culture device.	urine samples from 1,003 pediatric patients were examined for bacteriuria with a culture-nitrite strip test, along with microscopic assessment of pyruia and conventional chemical analysis. the latter two methods proved of little value in reliably detecting or excluding substantial bacteriuria. an overall prevalence of 5.2% was shown. using microstix reagent strips, 92 urine samples with substantial colony counts were detected. subculture from the strips disclosed that 52 of these involved single ...	1975	808961
intermittent neutrophil-monocyte bactericidal defects in a patient with sarcoidosis.	reccurrent abnormalities of polymorphonuclear leukocyte and monocyte bactericidal activity were demonstrated in a patient with sarcoidosis. defective function occurred during hypercalcemia complicating recovery from listeria meningitis, and during separate, unrelated episodes of erythema nodosum, staphylococcal cellulitis, and pneumococcal pneumonia. leukocyte morphology, oxidative metabolism, degranulation, and content of myeloperoxidase and lysozyme were normal, but low leukocyte alkaline phos ...	1975	808991
physiological and environmental control of gram negative bacteria on skin.	the relative importance of the environment versus the physiology of the host in the control of gram negative bacteria on skin has been examined by two experimental designs. (i) the changes in incidence of gram negative carriage from three skin sites in a climate controlled chamber at 35 degrees c and 90% humidity for 64 h were determined. following 2 weeks of tetracycline treatment the above experiment was repeated. high temperatures and humidity increased the overall frequency of isolation of g ...	1975	809052
antibacterial activity of human cervical mucus.	the antimicrobial property of human uterine cervical mucus was tested in three groups of women. healthy women, using no contraception, women using an intrauterine device and women receiving hormonal treatment for contraception. cervical mucus was taken on the 10th, 14th, 18th and 22nd day of the menstrual cycle. cervical mucus had a strong inhibitory effect on the growth of micrococcus lysodeicticus in all three groups. the strength of the inhibitory effect on the other microorganisms were in t ...	1975	809320
the antibacterial activity of a new cephalosporin, defamandole.		1975	809407
clinical, pharmacokinetic and laboratory study of penicillin v in the treatment of acute urinary infection.		1975	809408
cholesterol incorporation into bacterial membranes.	the wall-covered bacteria micrococcus lysodeikticus, bacillus megaterium, and proteus mirabilis incorporated exogenous cholesterol into their cytoplasmic membrane in quantities resembling those incorporated by sterol-nonrequiring mycoplasmas. cholesterol incorporation into the outer membrane of p. mirabilis was much more restricted than into the cytoplasmic membrane.	1975	809427
endemic infection in surgical wards.	endemic infection in male surgical wards has been studied during three periods. there was some infection due to gram-negative bacilli, though staphylococcus aureus remained as the single most important pathogen even in the absence of epidemic spread. beta haemolytic streptococci were isolated in large numbers from the lesions of four patients with deep wound infection. changes introduced in the pattern of post-operative care reduced sepsis due to staph. aureus, reduced the severity of wound infe ...	1975	809499
[proceedings: septicemia and menigitis in the newborn].		1975	809746
effect of consecutive antibacterial therapy on bacteriuria in hospitalized geriatric patients.	the effects of treatment with a consecutive series of different antibacterial drugs, most of them commonly used in urinary tract infections, were studied in 36 hospitalized geriatric patients with bacteriuria. most of the patients had advanced handicaps and all had urinary incontinence. urinary acidifying drugs, penicillin v, sulfonamide, nitrofurantoin, nalidixic acid, and trimethoprim/sulfamethoxazole were used as antibacterial agents. the general conclusion was that even the administration of ...	1975	809837
antimicrobial activity of alkaloids from amphibian venoms and effects on the ultrastructure of yeast cells.		1975	809864
endophthalmitis following cataract extraction. a study of 24 cases in 4,498 operations.	the incidence of postoperative endophthalmitis in a series of 4,498 consecutive cataract operations, performed in a period of 10 years, was examined and found to equal 0.533%. paracentesis of the anterior chamber was done in two cases and revealed staphylococcus albus to be the causative organism in both instances. conjunctival cultures were recovered in a further 17 cases, and showed s. albus in pure culture in eight cases and in combination with pseudomonas aeruginosa and proteous morgani in t ...	1975	809983
prevalence of gram-negative rods in the normal pharyngeal flora.	we obtained throat cultures from 100 randomly selected people free from any chronic upper or lower respiratory disease who did not work in a hospital and who had not experienced any acute illness or received any antibacterial therapy in the 4 weeks preceding culture. eighteen percent harbored either a species of enterobacteriaceae or pseudomonas aeruginosa in their pharynx. in all cases, colony counts were low, the majority being detected in broth media selective for gram-negative rods. there we ...	1975	810051
susceptibilities of gram-negative bacteria to combinations of antimicrobial agents in vitro.	combinations of either clindamycin or erythromycin with gentamicin, colimycin, or carbenicillin were studied for their antimicrobial effects on clinical isolates of escherichia coli, klebsiella sp., proteus mirabilis, serratia marcescens, enterobacter aerogenes, and pseudomonas aeruginosa. no antagonistic effects of the antibiotic combinations were observed. synergistic effects of the antibiotic combinations were noted against most strains of e. coli tested, especially when either clindamycin or ...	1975	810077
disk diffusion and serial dilution tests of susceptibility of some pathogenic gram-negative bacilli and enterococci to carbenicillin and ampicillin.	tests for susceptibility to ampicillin and carbenicillin were performed with 35 strains each of klebsiella, enterobacter, serratia, and proteus, 71 strains of pseudomonas aeruginosa, and 68 strains of enterococci by serial dilution and disk-diffusion tests employing 10(-3) dilutions of overnight cultures as inocula for both. commercial 10-mug ampicillin and 50- and 100-mug carbenicillin disks, and freshly prepared 10-, 50-, and 75-mug ampicillin and 10- and 50-mug carbenicillin disks were used. ...	1975	810078
antibacterial activity of nitropyrroles, nitrothiphenes, and aminothiophenes in vitro.	the antibacterial activities of nitropyrroles, nitrothiophenes, and aminothiophenes were studied. replacement of the nitro group with an amino group enhanced the activity of the thiophene compounds.	1975	810079
[new development on the field of antibiotic therapy].		1975	810448
the antibacterial and pharmacological activity of oxolinic acid (prodoxol).		1975	810473
[the antibiotic effect of the madder root (rubia tinctorium)].		1975	810813
a theoretical approach of disinfectant testing.	the purpose of this study is to compare six methods of disinfectant testing, currently applied in europe. the testing techniques are analysed into the separate elements, which can influence the activity of the disinfectant. the differences observed in these components allow to explain why in each method other parameters are evaluated and consequently, why the interpretation of the results varies from method to method. the six tests examined are (1) the suspension test of the dghm, (2) the suspen ...	1975	811003
[occurrence and function of a proteinase inhibitor in the hemolymph of insects].	the presence of proteinase inhibitor has been proved in the hemolymph of a number of insect species from seven different insect orders. the amount of proteinase inhibitor in the hemolymph significantly increases after injection of inactivated bacteria into the hemocoelom of galleria mellonella-larvae. moreover the larvae show an increased resistance against normally lethal concentrations of trypsin, chymotrypsin, pronase p and extracellular proteinase produced by pseudomonas aeruginosa. it is di ...	1975	811027
attempted reversal of oxytetracycline resistance of proteus mirabilis by edta-tromethamine lavage in experimentally induced canine and feline cystitis.	an attempt was made to reverse the antibiotic resistance of proteus mirabilis used to induce experimental cystitis in cats and dogs. results of in vitro studies on an oxytetracycline-resistant strain of p mirabilis, utilizing standard plate counts, indicated the organism became susceptible to oxytetracycline when it was treated with a solution of edta-tromethamine. in vivo experiments were conducted with cats and dogs in which 50% of the experimentally infected animals were treated with bladder ...	1975	811139
bacteriophage typing of gram-negative rod-shaped bacteria.		1975	811424
in vitro activity of ticarcillin, carbenicillin and ampicillin against some gram-negative bacilli.	alpha-carboxy-3-thienylmethyl penicillin (ticarcillin) is a relatively new semisynthetic penicillin which is more active than carbenillin against pseudomonas aeruginosa. among the strains tested, those isolated from the respiratory tract showed an increased susceptibility to carbenicillin and ticarcillin. as with carbenicillin, synergistic activity against p. aeruginosa could be demonstrated with ticarcillin in combination with gentamicin. like other penicillins, the antibacterial activity was i ...	1975	811636
thin-layer chromatographic technique for rapid detection of bacterial phospholipases.	silica gel thin-layer chromatography was employed to detect lecithinase activity induced from bacterial resting cell preparations induced from bacterial resting cell preparations incubated at 37 c for 4 h in the presence of purified egg yolk lecithin. bacillus subtilis, bacillus cereus, serratia marcescens, and pseudomonas aeruginosa hydrolyzed lecithin with the formation of free fatty acids as the sole lipid-soluble product. in none of the escherichia coli and citrobacter freundii strains teste ...	1975	811682
ecological studies of the microbial flora of the female lower genital tract.		1975	812101
[results of swimming-pool-filter examination].		1975	812177
nationwide survey of antibiotic resistance by means of a computer. an introduction into the analysis of multiple antibiotic resistance.	one-year surveillance of single and multiple antibiotic resistance of 8 species of so-called "problem bacteria" by computer analysis of data obtained from 18 public health laboratories in slovakia in 1973 enables to establish the most frequently occuring spectra of multiple drug resistance which would point to the spread of typical r plasmids among the problem bacteria investigated. as an introduction, we surveyed the mode of testing of problem bacteria in individual phls against antibiotics - d ...	1975	812292
nation-wide survey of antibiotic resistance by means of a computer: bacterial strains from the urine, stool, and upper respiratory tract.	all-nation data of antibiotic resistance of about 200 000 bacterial strains isolated in the slovak socialist republic in 1973 have been grouped, and compared, by means of a computer, into resistances of strains isolated from the urine, from upper respiratory tract (urt), from stool and from other pathological materials. in general, urine strains of all species investigated were found to be more resistant to "main antibiotics", and, suprisingly, to septrin, than strains from other sources. strain ...	1975	812295
[test methods for surgical hand disinfection (author's transl)].	as exemplified by a test preparation, methods for assessing the effect of hand disinfection on the resident flora are put up for discussion. the test of a method for hand disinfection must make allowance for conditions prevailing in practice. accordingly, the following steps should be taken into consideration: a. single use 1. immediate disinfecting effect 2. duration of the disinfecting effect b. repeated use 3. course of the initial colony count 4. immediate disinfecting effect 5. behaviour o ...	1975	812297
[microflora and immunity in surgical patients. i. some microfloral indices in the pre- and postoperative periods].	surgical treatment can lead to the appearance of a purulent staphylococcus infection of endogenous etiology. endogenous infection originates as a result of the patient's diminished resistance, indicated by an increase in the number of pathogenic staphylococci, the appearance of representatives of the intestinal flora in the oral cavity and on the surface of the skin. no iogenous purulent infection.	1975	812300
[biocenotic dynamics of liquid sewage in the process of its biological purification at aeration stations].	a study was made of biological purification of sewage at the aeration stations on the quantitative composition of the main indicator microbes--of bacteria of the coliform group and of the fecal coliform bacilli, enterococci, proteus, and also pathogenic enterobacteria. there was found a difference in the behaviour of different species of proteus, i.e. reduction in the process of purification in the numbers of pr. mirabilis, and a sharp elevation of pr. morganii content. there was noted an insign ...	1975	812301
combined activity of clindamycin and gentamicin on bacteroides fragilis and other bacteria.		1975	812858
[post-partum infectious sacro-iliitis caused by "commonplace bacteria" (proteus mirabilis?)].		1975	788081
functional modifications and nuclear analogues of beta-lactam antibiotics--part i.		1975	781739
[transition of dna with r factor nr84].		1975	765551
[bactericidal activity of serum and chemotherapy in sensitive and resistant exciter (author's transl)].	comparing examinations with ampicillin sensitive and resistant bacteria-strains show that the bactericidal activity of serum is dependent on the bacteria-strains, on the ampicillin sensitivity of the particular exciter and on the number of bacteria/ml (germ count). bactericide effect could always be obtained with sensitive strains as a result of additional chemotherapy. with several resistant strains a bactericide effect could not be obtained in this case the continuous optimal ampicillin addit ...	1975	765704
[new data on the karyology of formation of l forms of proteus vulgaris].	the growth and development of the proteus vulgaris culture as well as the process of formation of its l-forms were studied following the pitzurra method on slides covered with an agar layer. the latter were kept upon inoculation in a vertical position in a moist chamber at 37 degrees c. on fixed and stained after giemza preparations, it was possible to follow the consecutive stages of the cytoplasmic "hernia" formation due to the local disruption of the bacillary cell wall. it was easy to disern ...	1975	775258
gram negative septic shock.		1975	801269
enterobacterial chelators of iron: their occurrence, detection, and relation to pathogenicity.	in or on agar media, low-density seedings of enterobacteria fail to grow in the presence of certain concentrations of ethylene diamine-di-orthohydroxyphenyl acetic acid (edda); on the other hand, high-density seedings not only grow but secrete iron chelators which release the iron bound by the edda in the medium and stimulate the growth of low-density seedings. plates of media containing edda with low-density seedings of indicator organisms were used to survey iron-chelator production in seven e ...	1975	812996