Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the transcriptional programme of salmonella enterica serovar typhimurium reveals a key role for tryptophan metabolism in biofilms. | biofilm formation enhances the capacity of pathogenic salmonella bacteria to survive stresses that are commonly encountered within food processing and during host infection. the persistence of salmonella within the food chain has become a major health concern, as biofilms can serve as a reservoir for the contamination of food products. while the molecular mechanisms required for the survival of bacteria on surfaces are not fully understood, transcriptional studies of other bacteria have demonstr ... | 2009 | 20003355 |
| spray washing of tomatoes with chlorine dioxide to minimize salmonella on inoculated fruit surfaces and cross-contamination from revolving brushes. | chlorine dioxide (clo(2)) is an antimicrobial agent available for commercial produce washing. this study examined the efficacy of clo(2) at 5 parts per million (ppm) during spray washing of tomatoes (5.0 ml/s per fruit) for preventing salmonella enterica transfer from inoculated roller brushes to fruit and wash runoff. furthermore, the sanitizing effects of clo(2) during spray washing at low and high flow rates (5.0 and 9.3 ml/s per fruit, respectively) on tomato surfaces (nonstem scar areas) wi ... | 2009 | 20003724 |
| enteric fever in mediterranean north africa. | typhoid fever is endemic in the mediterranean north african countries (morocco, algeria, tunisia, libya, and egypt) with an estimated incidence of 10-100 cases per 100,000 persons. outbreaks caused by salmonella enterica serovar typhi are common and mainly due to the consumption of untreated or sewage-contaminated water. salmonella enterica paratyphi b is more commonly involved in nosocomial cases of enteric fever in north africa than expected and leads to high mortality rates among infants with ... | 2009 | 20009276 |
| clonal dissemination of the multi-drug resistant salmonella enterica serovar braenderup, but not the serovar bareilly, of prevalent serogroup c1 salmonella from taiwan. | nontyphoidal salmonella is the main cause of human salmonellosis. in order to study the prevalent serogroups and serovars of clinical isolates in taiwan, 8931 salmonellae isolates were collected from 19 medical centers and district hospitals throughout the country from 2004 to 2007. the pulsed-field eletrophoresis types (pfge) and antibiotic resistance profiles of salmonella enterica serovars bareilly (s. bareilly) and braenderup (s. braenderup) were compared, and multi-drug resistance (mdr) pla ... | 2009 | 20017951 |
| transcriptional response in the peripheral blood of patients infected with salmonella enterica serovar typhi. | we used microarrays and transcriptional profiling of peripheral blood to investigate the host response of 29 individuals who contracted typhoid fever in the mekong delta region of vietnam. samples were taken over a nine month period encompassing acute disease, convalescence, and recovery. we found that typhoid fever induced a distinct and highly reproducible signature in the peripheral blood that changed during treatment and convalescence, returning in the majority of cases to the "normal" profi ... | 2009 | 20018727 |
| virulence potential of five major pathogenicity islands (spi-1 to spi-5) of salmonella enterica serovar enteritidis for chickens. | salmonella is a highly successful parasite of reptiles, birds and mammals. its ability to infect and colonise such a broad range of hosts coincided with the introduction of new genetic determinants, among them 5 major pathogenicity islands (spi1-5), into the salmonella genome. however, only limited information is available on how each of these pathogenicity islands influences the ability of salmonella to infect chickens. in this study, we therefore constructed salmonella enteritidis mutants with ... | 2009 | 20021686 |
| investigation of a community outbreak of typhoid fever associated with drinking water. | this report is about the investigation of an outbreak of typhoid fever claimed three human lives and left more than 300 people suffered within one week. the aim of this report is to draw the attention of global health community towards the areas that are still far from basic human essentialities. | 2009 | 20021691 |
| genotyping, plasmid analysis, and antimicrobial susceptibility of salmonella enterica serotype enteritidis isolates from humans and chickens in central taiwan. | salmonella enterica serotype enteritidis (se) is the most frequent etiological agent of human salmonellosis. the molecular epidemiology and antimicrobial susceptibility of human and chicken isolates of se were examined. | 2009 | 19864196 |
| tracing lineage by phenotypic and genotypic markers in salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- and salmonella typhimurium isolated in state of são paulo, brazil. | fifty-three salmonella 1,4,[5],12:i:- and 45 salmonella typhimurium strains were characterised using phage typing, plasmid profiles and pulsed-field gel electrophoresis (pfge) for comparison. the majority of the strains were subdivided into definitive type (dt) 41 (22.6%) and dt 193 (18%) and the 60-mda plasmid was detected in 94.3% and 84.4% of strains, respectively. genetic diversity was observed among all strains and 90% presented a > 70% similarity through pfge analysis. these results sugges ... | 2009 | 20027476 |
| molecular characterization of the multidrug-resistant phage types salmonella enterica serovar typhimurium dt104, dt20a and dt120 strains in the slovakia. | an increase in the number of multidrug-resistant salmonella enterica serovar typhimurium strains (definitive phage type dt20a and dt120) as well as the occurrence of dt104 strains during 2003-2005 in slovakia was documented. based on the results of the molecular analysis we suggest that multidrug-resistant dt20a and dt120 phage types are more closely related to multidrug-resistant phage type, and that the occurrence is probably due to changes in the phage susceptibility of dt104. continued surve ... | 2009 | 17306968 |
| potent anti-tumor activity of systemically-administered il2-expressing salmonella correlates with decreased angiogenesis and enhanced tumor apoptosis. | salmonella enterica serovar typhimurium (hereafter s. typhimurium) stains have been shown to exert a potent inhibitory effect on the growth of human and mouse tumors in experimental models. our laboratory has previously demonstrated that an attenuated strain of s. typhimurium engineered to express il2 (designated strain gidil2) has demonstrable immunopotentiating properties, particularly affecting the innate arm of the immune system. in the present study, we wished to explore the properties of i ... | 2009 | 18849195 |
| interaction of salmonella enterica with basil and other salad leaves. | contaminated salad leaves have emerged as important vehicles for the transmission of enteric pathogens to humans. a recent outbreak of salmonella enterica serovar senftenberg (s. senftenberg) in the united kingdom has been traced to the consumption of contaminated basil. using the outbreak strain of s. senftenberg, we found that it binds to basil, lettuce, rocket and spinach leaves showing a pattern of diffuse adhesion. flagella were seen linking s. senftenberg to the leaf epidermis, and the del ... | 2009 | 18830276 |
| reduction in infection risk through treatment of microbially contaminated surfaces with a novel, portable, saturated steam vapor disinfection system. | surface-mediated infectious disease transmission is a major concern in various settings, including schools, hospitals, and food-processing facilities. chemical disinfectants are frequently used to reduce contamination, but many pose significant risks to humans, surfaces, and the environment, and all must be properly applied in strict accordance with label instructions to be effective. this study set out to determine the capability of a novel chemical-free, saturated steam vapor disinfection syst ... | 2009 | 18834748 |
| distribution of integrons and sgi1 among antibiotic-resistant salmonella enterica isolates of animal origin. | in salmonella enterica, resistance to antibiotics can be caused by the presence of sgi1, transposons or conjugative plasmids. in this study we were interested in the relative contribution of these genetic elements to the antibiotic resistance of s. enterica isolates collected within a single year in the czech republic from animal sources. altogether 123 antibiotic-resistant isolates belonging to 16 different s. enterica serovars were classified into 3 groups according to the presence of sgi1 and ... | 2009 | 18723300 |
| salmonella infection of a penile prosthesis. | patients with erectile dysfunction (ed) who have failed oral or intracavernosal erectogenic therapy may benefit from inflatable penile prosthesis (ipp) placement. such individuals commonly include patients with diabetes mellitus or chronic renal insufficiency. complications of ipp placement include erosion and infection. most often, infection of ipps involves skin flora that are hypothesized to be introduced at the time of device placement and persist as a biofilm, causing clinical infection as ... | 2009 | 18823319 |
| salmonella indiana as a cause of abortion in ewes: genetic diversity and resistance patterns. | salmonella enterica subspecies enterica indiana, a food-borne serovar uncommon in most countries, was responsible for an outbreak of abortion in a flock of lacaune dairy ewes in southern spain. drinking water and feedstuff samples were analysed in an attempt to determine the source of the infection. pigeons (columba livia) and turtledoves (streptopelia turtur) in close contact with the ewes were captured and examined for the bacterium. seventeen s. indiana strains were isolated from the ewes and ... | 2009 | 18823722 |
| abelson tyrosine kinase facilitates salmonella enterica serovar typhimurium entry into epithelial cells. | the intracellular gram-negative bacterial pathogen salmonella enterica serovar typhimurium gains entry into nonphagocytic cells by manipulating the assembly of the host actin cytoskeleton. s. enterica serovar typhimurium entry requires a functional type iii secretion system, a conduit through which bacterial effector proteins are directly translocated into the host cytosol. we and others have previously reported the enhancement of tyrosine kinase activities during salmonella serovar typhimurium ... | 2009 | 18936177 |
| modulation of macrophage proinflammatory functions by cytokine-expressing salmonella vectors. | we previously reported that the intraperitoneal administration of recombinant strains of salmonella enterica serovar typhimurium, engineered to express murine il-2 (designated gidil2) or ifn-gamma (gidifngamma), induced a cytokine-specific modulation of the host innate immune response. interestingly, the bacteria-expressed cytokines were not secreted, but instead were associated with the bacterial cytosol. to understand the mechanism by which these two transfectants influence immune cells, we in ... | 2009 | 18835224 |
| predicting salmonella enterica serotypes by repetitive sequence-based pcr. | repetitive extragenic palindromic sequence-based pcr (rep-pcr) utilizing a semi-automated system, was evaluated as a method to determine salmonella serotypes. a group of 216 salmonella isolates belonging to 13 frequently isolated serotypes and one rarer serotype from poultry were used to create a dna fingerprint library with the diversilab system software. subsequently, a blinded set of 44 poultry isolates were fingerprinted and queried against the library in an attempt to putatively assign a se ... | 2009 | 18835303 |
| fluorescent bio-barcode dna assay for the detection of salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis is one of the most frequently reported causes of foodborne illness. it is a major threat to the food safety chain and public health. a highly amplified bio-barcode dna assay for the rapid detection of the insertion element (iel) gene of salmonella enteritidis is reported in this paper. the biosensor transducer is composed of two nanoparticles: gold nanoparticles (au-nps) and magnetic nanoparticles (mnps). the au-nps are coated with the target-specific dna ... | 2009 | 18835708 |
| early immune response following salmonella enterica subspecies enterica serovar typhimurium infection in porcine jejunal gut loops. | salmonella enterica subspecies enterica serovar typhimurium, commonly called s. typhimurium, can cause intestinal infections in humans and various animal species such as swine. to analyze the host response to salmonella infection in the pig we used an in vivo gut loop model, which allows the analysis of multiple immune responses within the same animal. four jejunal gut-loops were each inoculated with 3 x 10(8) cfu of s. typhimurium in 3 one-month-old piglets and mrna expressions of various cytok ... | 2009 | 18922229 |
| human polymorphonuclear neutrophil responses to burkholderia pseudomallei in healthy and diabetic subjects. | the major predisposing factor for melioidosis is diabetes mellitus, but no immunological mechanisms have been investigated to explain this. in this study, polymorphonuclear neutrophil (pmn) responses to burkholderia pseudomallei, the causative agent of melioidosis, in healthy and diabetic thai subjects were determined by flow cytometry. the results showed that b. pseudomallei displayed reduced uptake by pmns compared to salmonella enterica serovar typhimurium and escherichia coli. additionally, ... | 2009 | 18955471 |
| interleukin-23 orchestrates mucosal responses to salmonella enterica serotype typhimurium in the intestine. | salmonella enterica serotype typhimurium causes an acute inflammatory reaction in the ceca of streptomycin-pretreated mice that involves t-cell-dependent induction of gamma interferon (ifn-gamma), interleukin-22 (il-22), and il-17 expression (genes ifn-gamma, il-22, and il-17, respectively). we investigated here the role of il-23 in initiating these inflammatory responses using the streptomycin-pretreated mouse model. compared to wild-type mice, the expression of il-17 was abrogated, il-22 expre ... | 2009 | 18955477 |
| non-typhoidal salmonella septic arthritis in an immunocompetent child with a pharyngeal streptococcal infection. | we report the case of an immunocompetent child who showed monoarticular arthritis and fever, preceded by pharyngitis and arthralgias. because group a beta-hemolytic streptococcus had been detected in the pharyngeal swab, erythromycin was given on admission. however, based on ultrasound examination, therapy with ceftriaxone and joint fluid drainage were promptly performed, and a rapid and full recovery followed. meanwhile, salmonella enterica infection was revealed in blood and joint fluid. our c ... | 2009 | 18707909 |
| ciprofloxacin-resistant salmonella enterica serovar typhi from kuwait with novel mutations in gyra and parc genes. | blood isolates of salmonella enterica serovar typhi from two recently returned bangladeshi patients in kuwait were ciprofloxacin resistant, with ciprofloxacin mics of 12 mg/liter for both isolates. both isolates had three novel gyra mutations (55-leu-->trp, 87-asp-->ala, and 106-gln-->arg) and three novel parc mutations (84-glu-->lys, 106-trp-->gly, and 128-tyr-->asp). | 2009 | 18971360 |
| salmonella infections in antarctic fauna and island populations of wildlife exposed to human activities in coastal areas of australia. | salmonella infections in antarctic wildlife were first reported in 1970 and in a search for evidence linking isolations with exposure to human activities, a comparison was made of serovars reported from marine fauna in the antarctic region from 1982-2004 with those from marine mammals in the northern hemisphere. this revealed that 10 (83%) salmonella enterica serovars isolated from antarctic penguins and seals were classifiable in high-frequency (hf) quotients for serovars prevalent in humans an ... | 2009 | 18789175 |
| clya cytolysin from salmonella: distribution within the genus, regulation of expression by slya, and pore-forming characteristics. | functional homologs of the escherichia coli cytolysin a (clya, hlye, shea) gene have recently been detected in salmonella enterica serovars typhi (s. typhi) and paratyphi a (s. paratyphi a). in this study, analysis of a collection of salmonella strains showed that all s. typhi and s. paratyphi a strains tested harbor an intact copy of the corresponding clya variant, i.e. clya(sty) and clya(spaa), respectively. on the other hand, clya proved to be absent in the s. enterica serovar paratyphi b and ... | 2009 | 18715828 |
| multiple-locus variable-number tandem repeat analysis for subtyping of salmonella enterica subsp. enterica serovar enteritidis. | salmonella enterica subsp. enterica serovar enteritidis is a major food-borne pathogen that caused most of salmonella infections worldwide. s. enteritidis phage type 4 (pt4) especially presents a real challenge for the classical typing methods. we developed a simple multiple-locus variable-number tandem repeat analysis (mlva) assay based on three hypervariable variable-number tandem repeat (vntr) loci for subtyping of salmonella enteritidis. testing an arbitrary chosen strain collection of 110 s ... | 2009 | 18706857 |
| improvement of the live vaccine strain salmonella enterica serovar typhi ty21a for antigen delivery via the hemolysin secretion system of escherichia coli. | the attenuated salmonella enterica serovar typhi strain ty21a (ty21a) is the only attenuated live oral vaccine against typhoid fever. ty21a is also an attractive carrier for the delivery of heterologous antigens. we have used ty21a for antigen delivery via the hemolysin (hlya) secretion system of escherichia coli, the prototype of the type i secretion system (t1ss). in this study, we identified by genetic complementation that the specific mutation of rpos correlated with the hemolysin production ... | 2009 | 18706861 |
| the in vitro anti-pathogenic activity of immunoglobulin concentrates extracted from ovine blood. | an immunoglobulin-rich fraction has been prepared from ovine blood in our laboratory. we have investigated its antibacterial activity and binding activity to pathogenic whole cell antigens, lipopolysaccharide (lps) and staphylococcal enterotoxin b. ovine immunoglobulin concentrate (oic) comprised about 73 +/- 2% of igg and 11 +/- 1% of igm on a protein basis. it inhibited the growth of all 13 strains of pathogens tested, but the inhibitory activity varied according to bacterial strain. the inhib ... | 2009 | 18769875 |
| the use of maplt and mlva analyses of phenotypically closely related isolates of salmonella enterica serovar typhimurium. | the resolving power of multiple-locus variable-number tandem repeat analysis (mlva) was undertaken on 78 phenotypically closely related isolates of salmonella enterica serovar typhimurium that were previously analysed with multiple amplification of phage locus typing (maplt) and pulsed-field gel electrophoresis (pfge). isolates were tested with 10 primer sets targeting tandem repeat loci in the s. typhimurium genome. a new primer set targeting the sb21(st64b) locus was also assessed for maplt an ... | 2009 | 18617439 |
| mitracarpus frigidus aerial parts exhibited potent antimicrobial, antileishmanial, and antioxidant effects. | the crude extract and the hexane, ch(2)cl(2), etoac, n-buoh, and hydromethanolic fractions of the aerial parts of mitracarpus frigidus were evaluated against promastigote forms of two species of leishmania (l. chagasi and l. amazonensis), 11 strains of bacteria (staphylococcus aureus, pseudomonas aeruginosa, salmonella enterica sorovar tythimurium, shigella sonnei, klebsiella pneumoniae, escherichia coli, micrococcus luteus, enterococcus faecalis, enterobacter cloacae, streptococcus pyogenes and ... | 2009 | 18644325 |
| molecular analysis of high-level ciprofloxacin resistance in salmonella enterica serovar typhi and s. paratyphi a: need to expand the qrdr region? | fourteen strains of s. typhi (n=13) and s. paratyphi a (n=1) resistant to ciprofloxacin were compared with 30 ciprofloxacin decreased-susceptibility strains on the basis of qnr plasmid analysis, and nucleotide substitutions at gyra, gyrb, parc and pare. in ciprofloxacin-resistant strains, five s. typhi and a single s. paratyphi a showed triple mutations in gyra (ser83-->phe, asp87-->asn, glu133-->gly) and a novel mutation outside the quinolone resistance determining region (qrdr) (met52-->leu). ... | 2009 | 18687156 |
| a multi-state outbreak of salmonella saintpaul in australia associated with cantaloupe consumption. | a multi-state outbreak of salmonella enterica serovar saintpaul infection occurred in australia during october 2006. a case-control study conducted in three affected jurisdictions, new south wales, victoria and australian capital territory, included 36 cases with the outbreak-specific strain of s. saintpaul identified by multiple locus variable-number tandem repeat analysis (mlva) in a faecal specimen and 106 controls. consumption of cantaloupe (rockmelon) was strongly associated with illness (a ... | 2009 | 18559128 |
| disease threats to the endangered iberian lynx (lynx pardinus). | the iberian lynx, (lynx pardinus), is the most endangered felid in the world. to determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (vulpes vulpes), 24 egyptian mongooses (herpestes ichneumon), 10 common genets (genetta genetta) and 2 eurasian badgers (meles meles) in the are ... | 2009 | 18555712 |
| survival of selected pathogens in diluted sludge of a thermophilic waste treatment plant and in nacl-solution under aerobic and anaerobic conditions. | decimal reduction times (drt or d-value) of campylobacter jejuni, salmonella enterica (formerly salmonella choleraesuis) serovar senftenberg, escherichia coli, and listeria monocytogenes were determined in two different matrices, diluted fermenter sludge (dfs) and 0.95% nacl-solution (nacl) at 50 degrees c, both under aerobic and anaerobic conditions. depending on aeration, matrix composition, and the respective organism, the d-values varied between 10min and more than 15h. generally the viabili ... | 2009 | 18445518 |
| acid pre-adaptation enhances virulence of salmonella enterica serovar typhimurium dam mutant. | it is well established that success or failure of bacterial pathogens during infection relies upon its ability to overcome many lethal environments in the host such as acidity, osmolarity and bile salts. in the present study, we have studied the effects of acid adaptation on the virulence of salmonella enterica serovar typhimurium dam mutant. our results indicated that ld(50) of adapted strains were lower than those of control strains. also, the in vivo assays have shown that the development of ... | 2009 | 18456425 |
| multiplex pcr for differential diagnosis of emerging typhoidal pathogens directly from blood samples. | classically salmonella enterica serovar typhi (s. typhi) is associated with typhoid, a major health problem in developing countries. however, in recent years s. paratyphi a and vi-negative variants of s. typhi have emerged rapidly. we have developed a nested multiplex pcr targeting five different genes for differential diagnosis of typhoidal pathogens which has been optimized to be directly applicable on clinical blood samples. of 42 multiplex pcr-positive blood samples, 26, nine, and two were v ... | 2009 | 18413005 |
| surveillance of antimicrobial resistance of salmonella enterica serotype typhi in seven asian countries. | two hundred and four salmonella enterica serotype typhi (s. typhi) isolates were collected from seven asian countries during 2002-2004. multidrug-resistant s. typhi (resistant to > or = 3 antibiotics) was detected in 84 (41.2%) isolates and 142 (69.6%) showed reduced susceptibility to ciprofloxacin (minimum inhibitory concentration=0.125-1.0 mg/l). this study highlights the worsening situation of antimicrobial resistance of s. typhi in asia. | 2009 | 18474127 |
| regulation of expression of the tricarballylate utilization operon (tcuabc) of salmonella enterica. | the tricarballylate utilization locus (tcurabc) of salmonella enterica serovar typhimurium is comprised of a 3-gene operon (tcuabc) that encodes functions that allow this bacterium to use tricarballylate as a source of carbon and energy, and the tcur gene, which encodes a putative lysr-type transcriptional regulator. in our studies, transcription of the tcuabc operon peaked at mid-log phase, and declined moderately during stationary phase. this pattern was not due to a change in the amount of tc ... | 2009 | 19284970 |
| an increase in enteric fever cases due to salmonella paratyphi a in & around chandigarh. | enteric fever is a major public health problem in india. it is classically caused by salmonella enterica serotype typhi. salmonella enterica serotype paratyphi a which had been reported less frequently from cases of enteric fever has shown an increasing trend since 1996 in india. there is also variation in the antimicrobial susceptibility of salmonella paratyphi a from different parts of the country. an attempt is therefore made to study the rate of isolation and antimicrobial susceptibility pat ... | 2009 | 19287065 |
| an additional novel antimicrobial resistance gene cluster in salmonella genomic island 1 of a salmonella enterica serovar typhimurium dt104 human isolate. | the multi-antimicrobial resistance gene cluster and its derivatives have been detected in salmonella genomic island 1 (sgi1), which has been identified in the salmonella enterica serovar typhimurium, phage types dt104, dt12, dt120, and u302, as well as other salmonella serovars, including agona, paratyphi b, albany, meleagridis, newport, cerro, derby, dusseldorf, infantis, kiambu, and emek. we acquired 53 salmonella typhimurium dt104 isolates from diarrheal patients in korea. from these isolates ... | 2009 | 19292580 |
| emergence, distribution, and molecular and phenotypic characteristics of salmonella enterica serotype 4,5,12:i:-. | salmonella spp. represent one of the most common causes of bacterial foodborne illnesses around the world. the species salmonella enterica contains more than 2500 serotypes, and emergence of new human pathogenic salmonella strains and serotypes represents a major public health issue. salmonella enterica subsp. enterica serotype 4,5,12:i:- represents a monophasic variant of salmonella typhimurium, which has rarely been identified before the mid-1990 s. the prevalence of this serotype among human ... | 2009 | 19292687 |
| characterization of pathogenic and resistant genome repertoire reveals two clonal lines in salmonella enterica subsp. enterica serovar paratyphi b (+)-tartrate positive. | a total of 36 contemporary human, animal, and environmental (+)-tartrate-fermenting (dt+) salmonella enterica serovar paratyphi b isolates, formerly called salmonella serovar java, and five related monophasic s. enterica serovar 4,5,12:b:- isolates from belgium, germany, the netherlands, and the united kingdom were investigated for clonality and antimicrobial resistance profiles, as well as their virulence and resistance gene repertoire. two major clonal lines, which could be phenotypically diff ... | 2009 | 19292689 |
| investigation of an outbreak of salmonella enterica serovar newport infection. | a large outbreak of salmonella enterica serotype newport infection occurred in northern ireland during september and october 2004. typing of isolates from patients confirmed that this strain was indistinguishable from that in concurrent outbreaks in regions of england, in scotland and in the isle of man. a total of 130 cases were distributed unequally across local government district areas in northern ireland. the epidemic curve suggested a continued exposure over about 4 weeks. a matched case-c ... | 2009 | 19296871 |
| differential il-23 requirement for il-22 and il-17a production during innate immunity against salmonella enterica serovar enteritidis. | early activation of the il-12/ifn-gamma axis has been shown following salmonella enterica serovar enteritidis (s. enteritidis) infection. we were interested to study whether il-22 and il-17a production is initiated early in response to s. enteritidis. we demonstrate here that il-22 was strongly elevated in the peritoneal lavage fluid and in serum already 1 day post-intraperitoneal infection (d.p.i.) of mice; not only il-22 but also il-17a was produced ex vivo by activated peritoneal exudate cell ... | 2009 | 19297659 |
| novel approach to control salmonella enterica by modern biophotonic technology: photosensitization. | salmonellosis is one of the most common foodborne diseases in the world. the aim of this study was to evaluate the antibacterial efficiency of 5-aminolevulinic acid (ala) based photosensitization against one of food pathogens salmonella enterica. | 2009 | 19302098 |
| phagocytic superoxide specifically damages an extracytoplasmic target to inhibit or kill salmonella. | the phagocytic oxidative burst is a primary effector of innate immunity that protects against bacterial infection. however, the mechanism by which reactive oxygen species (ros) kill or inhibit bacteria is not known. it is often assumed that dna is a primary target of oxidative damage, consistent with known effects of endogenously produced ros in the bacterial cytoplasm. but most studies fail to distinguish between effects of host derived ros versus damage caused by endogenous bacterial sources. ... | 2009 | 19305502 |
| mesoscopic and microstructural characterization of liposomes formed by the lipooligosaccharide from salmonella minnesota strain 595 (re mutant). | large unilamellar liposomes formed by the re lps (from here below named los) from salmonella enterica serotype minnesota strain 595 (re mutant) have been prepared by the extrusion techniques. the physico-chemical investigation of these systems has been performed by a combined experimental strategy, which has allowed a characterization at different observation scales, from the morphological to the micro-structural level. particularly, dynamic light scattering (dls) measurements have revealed the ... | 2009 | 19305906 |
| unusual class 1 integron-associated gene cassette configuration found in inca/c plasmids from salmonella enterica. | inca/c plasmids carrying an unusual cassette configuration in a class 1 integron and five further shared resistance genes, aacc4, apha1, hph, sul2, and teta(d) were found in salmonella enterica serovars senftenberg and ohio. a deletion formed using a short region of homology in the 5' conserved segment and the orff cassette created an array with only part of orff followed by the aada2 cassette. the inca/c plasmids were not recoverable by conjugation, but additional conjugative resistance plasmid ... | 2009 | 19307357 |
| omeprazole antagonizes virulence and inflammation in salmonella enterica-infected raw264.7 cells. | the proton pump inhibitor omeprazole reduced the intracellular replication of salmonella enterica serovar typhimurium in raw264.7 cells without affecting bacterial growth in vitro or the viability of the host cells. the mechanism was bacteriostatic and interfered with replication mediated by the virulence-associated spi2 type iii secretion system. the proton pump inhibitor bafilomycin a(1), in contrast, mediated killing of intracellular bacteria and imposed a marked cytotoxicity on raw264.7 cell ... | 2009 | 19307359 |
| invited review: breaking barriers--attack on innate immune defences by omptin surface proteases of enterobacterial pathogens. | the omptin family of gram-negative bacterial transmembrane aspartic proteases comprises surface proteins with a highly conserved beta-barrel fold but differing biological functions. the omptins ompt of escherichia coli, pgte of salmonella enterica, and pla of yersinia pestis differ in their substrate specificity as well as in control of their expression. their functional differences are in accordance with the differing pathogenesis of the infections caused by e. coli, salmonella, and y. pestis, ... | 2009 | 19318417 |
| microarray analysis of antimicrobial resistance genes in salmonella enterica from preharvest poultry environment. | to detect antimicrobial resistance genes in salmonella isolates from turkey flocks using the microarray technology. | 2009 | 19320942 |
| functions exerted by the virulence-associated type-three secretion systems during salmonella enterica serovar enteritidis invasion into and survival within chicken oviduct epithelial cells and macrophages. | salmonella enterica serovar enteritidis (se) infection of chickens is a major contributing factor to non-typhoidal salmonellosis. the roles of the type-three secretion systems (t3ss-1 and t3ss-2) in the pathogenesis of se infection of chickens are poorly understood. in this study, the functions of t3ss-1 and t3ss-2 during se infection of primary chicken oviduct epithelial cells (coec) and macrophages were characterized. the t3ss-1 and t3ss-2 mutants (sipb and ssav), impaired in translocation and ... | 2009 | 19322708 |
| in vitro evaluation of a new cefixime-clavulanic acid combination for gram-negative bacteria. | the study was conducted to evaluate a new cefixime-clavulanic acid combination for in vitro susceptibility towards gram-negative bacteria. a total of 220 isolates of escherichia coli, klebsiella pneumoniae, pseudomonas aeroginosa, acinetobacter spp, salmonella enterica serovar typhi and salmonella enterica serovar typhimurium were included in the study. the isolates were tested for susceptibility towards the new combination antimicrobial molecule cefixime with clavulanic acid by disk diffusion a ... | 2009 | 19323045 |
| role of pre-a motif in nitric oxide scavenging by truncated hemoglobin, hbn, of mycobacterium tuberculosis. | mycobacterium tuberculosis truncated hemoglobin, hbn, is endowed with a potent nitric-oxide dioxygenase activity and has been found to relieve nitrosative stress and enhance in vivo survival of a heterologous host, salmonella enterica typhimurium, within the macrophages. these findings implicate involvement of hbn in the defense of m. tuberculosis against nitrosative stress. the protein carries a tunnel system composed of a short and a long tunnel branch that has been proposed to facilitate diat ... | 2009 | 19329431 |
| interaction of candida albicans with an intestinal pathogen, salmonella enterica serovar typhimurium. | candida albicans is an opportunistic human fungal pathogen that normally resides in the gastrointestinal tract and on the skin as a commensal but can cause life-threatening invasive disease. salmonella enterica serovar typhimurium is a gram-negative bacterial pathogen that causes a significant amount of gastrointestinal infection in humans. both of these organisms are also pathogenic to the nematode caenorhabditis elegans, causing a persistent gut infection leading to worm death. in the present ... | 2009 | 19329669 |
| myxinidin, a novel antimicrobial peptide from the epidermal mucus of hagfish, myxine glutinosa l. | fish epidermal mucus contains innate immune components that provide a first line of defense against various infectious pathogens. this study reports the bioassay-guided fractionation and characterization of a novel antimicrobial peptide, myxinidin, from the acidic epidermal mucus extract of hagfish (myxine glutinosa l.). edman sequencing and mass spectrometry revealed that myxinidin consists of 12 amino acids and has a molecular mass of 1,327.68 da. myxinidin showed activity against a broad rang ... | 2009 | 19330556 |
| aquaculture and florfenicol resistance in salmonella enterica serovar typhimurium dt104. | 2009 | 19331756 | |
| emergence and evolution of multiply antibiotic-resistant salmonella enterica serovar paratyphi b d-tartrate-utilizing strains containing sgi1. | the first australian isolate of salmonella enterica serovar paratyphi b d-tartrate-utilizing (dt(+)) that is resistant to ampicillin, chloramphenicol, florfenicol, streptomycin, spectinomycin, sulfonamides, and tetracycline (apcmflsmspsutc) and contains sgi1 was isolated from a patient with gastroenteritis in early 1995. this is the earliest reported isolation globally. the incidence of infections caused by these sgi1-containing multiply antibiotic-resistant s. enterica serovar paratyphi b dt(+) ... | 2009 | 19332668 |
| genetic analysis of colistin resistance in salmonella enterica serovar typhimurium. | colistin is a cyclic cationic peptide that kills gram-negative bacteria by interacting with and disrupting the outer membrane. we isolated 44 independent mutants in salmonella enterica serovar typhimurium with reduced susceptibility to colistin and identified 27 different missense mutations located in the pmra and pmrb genes (encoding the regulator and sensor of a two-component regulatory system) that conferred increased resistance. by comparison of the two homologous sensor kinases, pmrb and en ... | 2009 | 19332669 |
| a simple broth-disk method to determine the minimum inhibitory concentration of ceftriaxone on salmonella enterica serovar typhi and paratyphi. | resistance to fluoroquinolones and cephalosporins is a major problem with salmonella enterica serovar typhi and paratyphi. an accurate determination of antibiotic susceptibility requires tests for minimum inhibitory concentration (mic) of antibiotics. we describe a simple broth-disk method to determine the mic of ceftriaxone on s. typhi and s. paratyphi. | 2009 | 19332908 |
| deep sequencing of salmonella rna associated with heterologous hfq proteins in vivo reveals small rnas as a major target class and identifies rna processing phenotypes. | the bacterial sm-like protein, hfq, is a key factor for the stability and function of small non-coding rnas (srnas) in escherichia coli. homologues of this protein have been predicted in many distantly related organisms yet their functional conservation as srna-binding proteins has not entirely been clear. to address this, we expressed in salmonella the hfq proteins of two eubacteria (neisseria meningitides, aquifex aeolicus) and an archaeon (methanocaldococcus jannaschii), and analyzed the asso ... | 2009 | 19333007 |
| identification of a flja gene on a linear plasmid as the repressor gene of flic in salmonella enterica serovar typhi. | salmonella enterica serovar typhi z66-positive strain contains an fljba-like operon on a linear plasmid and the flic gene on the chromosome encoding d or j antigen. the fljb-like gene has been identified as the gene encoding z66 antigen. to investigate the function of the flja-like gene in s. enterica serovar typhi, a z66-positive wild-type strain gifu10007 and its phase variation j positive strain 007j+ were used in the present study. after deletion of the flja-like gene, the wild-type strain t ... | 2009 | 19341422 |
| a novel hybrid yeast-human network analysis reveals an essential role for fnbp1l in antibacterial autophagy. | autophagy is a conserved cellular process required for the removal of defective organelles, protein aggregates, and intracellular pathogens. we used a network analysis strategy to identify novel human autophagy components based upon the yeast interactome centered on the core yeast autophagy proteins. this revealed the potential involvement of 14 novel mammalian genes in autophagy, several of which have known or predicted roles in membrane organization or dynamics. we selected one of these membra ... | 2009 | 19342671 |
| humoral immune responses inhibit the antitumor activities mediated by salmonella enterica serovar choleraesuis. | salmonella is a powerful antitumor agent owing to its tumor-targeting potential, antitumor capability, and ability to deliver therapeutic genes. host factors including innate and adaptive immune responses play roles in salmonella-induced antitumor activity. an emerging realization from animal models that host immune system responses to salmonella remains uncertain. herein, we used naive mice and salmonella enterica serovar choleraesuis (s. choleraesuis) immunized mice to study the role of humora ... | 2009 | 19342967 |
| an accelerated method for isolation of salmonella enterica serotype typhimurium from artificially contaminated foods, using a short preenrichment, immunomagnetic separation, and xylose-lysine-desoxycholate agar (6ix method). | rapid isolation of salmonella from food is essential for faster typing and source tracking in an outbreak. the objective of this study was to investigate a rapid isolation method that would augment the standard u.s. food and drug administration's bacteriological analytical manual (bam) method. food samples with low microbial load, including egg salad and ice cream, moderately high-microbial-load tomatoes, and high-microbial-load ground beef were intentionally inoculated with 2 to 48 cfu of salmo ... | 2009 | 19343948 |
| transfer of salmonella enterica serovar typhimurium from contaminated irrigation water to parsley is dependent on curli and cellulose, the biofilm matrix components. | enteric pathogens can contaminate fresh produce, and this contaminated produce can be a significant potential source of human illness. the objective of this study was to determine a possible mode of transfer of salmonella typhimurium from contaminated irrigation water to mature parsley plants and to investigate the role of bacterial cellulose and curli. parsley plants were drip irrigated with water containing green fluorescent protein-labeled salmonella typhimurium. stems and leaves were harvest ... | 2009 | 19343953 |
| roles of the outer membrane protein asma of salmonella enterica in the control of marrab expression and invasion of epithelial cells. | a genetic screen for suppressors of bile sensitivity in dna adenine methylase (dam) mutants of salmonella enterica serovar typhimurium yielded insertions in an uncharacterized locus homologous to the escherichia coli asma gene. disruption of asma suppressed bile sensitivity also in phop and wec mutants of s. enterica and increased the mic of sodium deoxycholate for the parental strain atcc 14028. increased levels of mara mrna were found in asma, asma dam, asma phop, and asma wec strains of s. en ... | 2009 | 19346309 |
| escherichia coli and salmonella enterica are protected against acetic acid, but not hydrochloric acid, by hypertonicity. | chapman et al. (b. chapman, n. jensen, t ross, and m. b. cole, appl. environ. microbiol. 72:5165-5172, 2006) demonstrated that an increased nacl concentration prolongs survival of escherichia coli o157 serl 2 in a broth model simulating the aqueous phase of a food dressing or sauce containing acetic acid. we examined the responses of five other e. coli strains and four salmonella enterica strains to increasing concentrations of nacl under conditions of lethal acidity and observed that the averag ... | 2009 | 19346344 |
| salmonella infection after surgery of small intestine foreign bodies. | to report the first case of salmonella enterica serotype choleraesuis infection after surgery for small intestine foreign bodies. | 2009 | 19349730 |
| effects of in-feed egg yolk antibodies on salmonella shedding, bacterial antibiotic resistance, and health of pigs. | to determine effects of anti-salmonella egg yolk antibodies on shedding and antibiotic resistance of salmonella enterica typhimurium, newly weaned pigs were randomly assigned to six dietary treatments. these treatment groups were (i) a control (basal) diet without additives and similar diets with (ii) egg yolk powder derived from chickens challenged with salmonella typhimurium antigens, (iii) a commercial egg yolk powder control, (iv) apramycin for 14 days followed by carbadox, (v) oxytetracycli ... | 2009 | 19350971 |
| contamination of bottles used for feeding reconstituted powdered infant formula and implications for public health. | microbial contamination of powdered infant formula (pif) is known to cause gastrointestinal infections in infants. of concern is intrinsic contamination of the formula with (for example) salmonella enterica as well as extrinsic contamination from inappropriate handling or ineffective disinfection. the aim of this study was to evaluate organic and microbial contamination of 'in-use' bottles used for feeding infants powdered formula milk in south wales, uk. | 2009 | 19354201 |
| characterisation of antibiotic resistance in host-adapted salmonella enterica. | salmonella enterica serovars dublin, choleraesuis and pullorum are host-adapted serovars that cause disease primarily in cattle, swine and poultry, respectively. in addition, serovars dublin and choleraesuis are important human pathogens that are disproportionately associated with severe invasive infections that require antimicrobial therapy. because of the potential increased emergence and spread of antimicrobial resistance, isolates of 42 s. enterica serovars dublin, choleraesuis and pullorum ... | 2009 | 19356907 |
| [molecular biological and phenotypical characterization of human isolates of salmonella enterica serovar paratyphi b dt+ or salmonella java]. | salmonella paratyphi b dt+ (or salmonella java) is an emerging public health problem. the study objective was to characterize phenotypically and genotypically 13 human isolates from sporadic cases of infection. | 2009 | 19358450 |
| reduction of salmonella enterica var. enteritidis colonization and invasion by bacillus cereus var. toyoi inclusion in poultry feeds. | the effect of continuously feeding the probiotic microorganism toyocerin to birds inoculated with salmonella enteritidis field-isolated strains on salmonella enteritidis prevalence, and performance variables were studied in 2 experiments. the experiments were performed with 1) broiler chickens in floor pens until slaughter 42 d of age, challenge was performed on d 3, 7, or 14 with 2 x 10(6) cfu per chick, and 2) single comb white leghorn chickens in cages until 28 d of age, challenge was perform ... | 2009 | 19359685 |
| salmonella enterica serovar enteritidis pathogenicity island 1 is not essential for but facilitates rapid systemic spread in chickens. | salmonella enterica subsp. enterica serovar enteritidis is a leading cause of human food-borne illness that is mainly associated with the consumption of contaminated poultry meat and eggs. to cause infection, s. enteritidis is known to use two type iii secretion systems, which are encoded on two salmonella pathogenicity islands, spi-1 and spi-2, the first of which is thought to play a major role in invasion and bacterial uptake. in order to study the role of spi-1 in the colonization of chicken, ... | 2009 | 19364835 |
| salmonella enterica serovar typhimurium pathogenicity island 1-encoded type iii secretion system translocases mediate intimate attachment to nonphagocytic cells. | delivery of bacterial proteins into mammalian cells by type iii secretion systems (ttss) is thought to require the intimate association of bacteria with target cells. the molecular bases of this intimate association appear to be different in different bacteria involving ttss components, as well as surface determinants not associated with ttss. we show here that the protein translocases sipb, sipc, and sipd of the salmonella enterica serovar typhimurium pathogenicity island 1 (spi-1)-encoded ttss ... | 2009 | 19364837 |
| o-antigen-negative salmonella enterica serovar typhimurium is attenuated in intestinal colonization but elicits colitis in streptomycin-treated mice. | lipopolysaccharide (lps) is a major constituent of the outer membrane and an important virulence factor of salmonella enterica subspecies 1 serovar typhimurium (serovar typhimurium). to evaluate the role of lps in eliciting intestinal inflammation in streptomycin-treated mice, we constructed an o-antigen-deficient serovar typhimurium strain through deletion of the wbap gene. the resulting strain was highly susceptible to human complement activity and the antimicrobial peptide mimic polymyxin b. ... | 2009 | 19364844 |
| relative transport behavior of escherichia coli o157:h7 and salmonella enterica serovar pullorum in packed bed column systems: influence of solution chemistry and cell concentration. | the influence of solution chemistry and cell concentration on bacterial transport has been examined using salmonella pullorum sa1685 and escherichia coli o157:h7. a column was employed to determine the transport behavior and deposition kinetics with aquifer sand over a range of ionic strengths and cell concentrations. o157:h7 was found to be more adhesive than sa1685, with calculated deposition rate coefficients higher than those of sa1685. comprehensive cell surface characterization techniques ... | 2009 | 19368180 |
| identification of resistance and virulence factors in an epidemic enterobacter hormaechei outbreak strain. | bacterial strains differ in their ability to cause hospital outbreaks. using comparative genomic hybridization, enterobacter cloacae complex isolates were studied to identify genetic markers specific for enterobacter cloacae complex outbreak strains. no outbreak-specific genes were found that were common in all investigated outbreak strains. therefore, the aim of our study was to identify specific genetic markers for an enterobacter hormaechei outbreak strain (ehos) that caused a nationwide outb ... | 2009 | 19372158 |
| [active efflux as the multidrug resistance mechanism]. | active efflux is a common resistance mechanism in a wide range of bacterial pathogens. it is responsible for the transport of such toxic compounds as drugs, toxins, and detergents. pumps with broad substrate profiles promote the emergence of multidrug-resistant bacterial pathogens that are a particular threat to contemporary medicine, such as pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, and salmonella enterica sv typhimurium. one can distinguish five major class of transport ... | 2009 | 19373193 |
| genetic basis of multidrug resistance in salmonella enterica serovars enteritidis and typhimurium isolated from diarrheic calves in egypt. | up to this date, nothing is known about the molecular basis of antimicrobial resistance in salmonella isolated from animals in africa. therefore, this study was carried out to screen the incidence of multidrug-resistant (mdr) strains of salmonella from neonatal calf diarrhea in egypt and also to characterize the molecular basis of this resistance. nine unique salmonella isolates were obtained from 220 fecal samples, and six of these showed multidrug resistance phenotypes and harbored at least tw ... | 2009 | 19375408 |
| salmonella induces flagellin- and myd88-dependent migration of bacteria-capturing dendritic cells into the gut lumen. | intestinal dendritic cells (dcs) sample bacteria, such as salmonella, by extending cellular processes into the lumen to capture bacteria and shuttle them across the epithelium; however, direct evidence of bacteria-loaded dcs travelling back into the tissue is lacking. we hypothesized that sampling is paralleled by migration of dcs into the lumen prior to or following the internalization of salmonella. | 2009 | 19375423 |
| effect of long chain fatty acids on salmonella killing, superoxide and nitric oxide production by chicken macrophages. | the objective of this study was to investigate the effect of uptake of different commonly consumed long chain fatty acids on superoxide (o(2)(-)), nitric oxide (no) production, and ability to kill salmonella enterica serotype typhimurium (s. typhimurium) by chicken macrophages (hd11 cells). all the fatty acids were taken up by hd11 cells with stearic acid uptake higher than polyunsaturated fatty acids. uptake of green fluorescent protein-labeled bacteria and the viability of hd11 cells (measured ... | 2009 | 19375809 |
| prevalence and concentration of verocytotoxigenic escherichia coli, salmonella enterica and listeria monocytogenes in the beef production chain: a review. | this review examines the prevalence of three important pathogens, verocytotoxigenic escherichia coli (vtec), salmonella enterica and listeria monocytogenes, in cattle and beef from the farm to the final, ready-to-eat product. factors affecting prevalence of pathogens in the beef chain, such as the season and cattle rearing method, are examined. data from many key surveys are summarized in table form. the observed prevalence of pathogens in cattle and beef varies considerably from survey to surve ... | 2009 | 19376457 |
| mutations in flk, flgg, flha, and flhe that affect the flagellar type iii secretion specificity switch in salmonella enterica. | upon completion of the flagellar hook-basal body (hbb) structure, the flagellar type iii secretion system switches from secreting rod/hook-type to filament-type substrates. the secretion specificity switch has been reported to occur prematurely (prior to hbb completion) in flk-null mutants (p. aldridge, j. e. karlinsey, e. becker, f. f. chevance, and k. t. hughes, mol. microbiol. 60:630-643, 2006) and in distal rod gene gain-of-function mutants (flgg* mutants) that produce filamentous rod struct ... | 2009 | 19376867 |
| a role for the eal-like protein stm1344 in regulation of csgd expression and motility in salmonella enterica serovar typhimurium. | the bacterial second messenger cyclic di-gmp (c-di-gmp) regulates the transition between sessility and motility. in salmonella enterica serovar typhimurium, the expression of csgd, the regulator of multicellular rdar morphotype behavior, is a major target of c-di-gmp signaling. csgd expression is positively regulated by at least two diguanylate cyclases, ggdef domain proteins, and negatively regulated by at least four phosphodiesterases, eal domain proteins. here, we show that in contrast to eal ... | 2009 | 19376870 |
| detection of live salmonella sp. cells in produce by a taqman-based quantitative reverse transcriptase real-time pcr targeting inva mrna. | salmonella enterica contamination in foods is a significant concern for public health. when dna detection methods are used for analysis of foods, one of the major concerns is false-positive results from the detection of dead cells. to circumvent this crucial issue, a taqman quantitative real-time rt-pcr (qrt-pcr) assay with an rna internal control was developed. inva rna standards were used to determine the detection limit of this assay as well as to determine inva mrna levels in mid-exponential ... | 2009 | 19376910 |
| role of ceftiofur in selection and dissemination of blacmy-2-mediated cephalosporin resistance in salmonella enterica and commensal escherichia coli isolates from cattle. | third-generation cephalosporin resistance of salmonella and commensal escherichia coli isolates from cattle in the united states is predominantly conferred by the cephamycinase cmy-2, which inactivates beta-lactam antimicrobial drugs used to treat a wide variety of infections, including pediatric salmonellosis. the emergence and dissemination of bla(cmy-2)(-)-bearing plasmids followed and may in part be the result of selection pressure imposed by the widespread utilization of ceftiofur, a third- ... | 2009 | 19376926 |
| glucose and glycolysis are required for the successful infection of macrophages and mice by salmonella enterica serovar typhimurium. | salmonella is a widespread zoonotic enteropathogen that causes gastroenteritis and fatal typhoidal disease in mammals. during systemic infection of mice, salmonella enterica serovar typhimurium resides and replicates in macrophages within the "salmonella-containing vacuole" (scv). it is surprising that the substrates and metabolic pathways necessary for growth of s. typhimurium within the scv of macrophages have not been identified yet. to determine whether s. typhimurium utilized sugars within ... | 2009 | 19380470 |
| predictive value of clinical and laboratory findings in the diagnosis of the enteric fever. | although the definitive diagnosis of enteric fever requires the isolation of salmonella enterica serotype typhi or paratyphi, the diagnosis is usually made according to clinical and laboratory findings. there is usually a diagnostic dilemma. the aim of this study was to determine the minimum required parameters that could be valuable in the diagnosis of enteric fever. a retrospective study was performed to compare the clinical and laboratory findings in 60 patients who proved to have enteric fev ... | 2009 | 19382666 |
| b cell precursors are targets for salmonella infection. | we previously reported that, in mice, b cells are a reservoir for bacteria during salmonella infection. here, we show that, within the bone marrow, b cells and their precursors are targeted for infection by salmonella enterica serovar typhimurium. our data suggest that b cells within the bone marrow may be a bacterial niche during chronic salmonella infection. | 2009 | 19383536 |
| fimh alleles direct preferential binding of salmonella to distinct mammalian cells or to avian cells. | this study aimed to determine whether allelic variants of the fimh adhesin from salmonella enterica confer differential bacterial binding to different types of mammalian cells [murine bone marrow-derived dendritic cells (dcs) and hep-2 cells] and chicken leukocytes. although the type 1 fimbriated s. enterica serovar typhimurium strains ajb3 (sr-11 derivative) and sl1344 both aggregated yeast cells, only the former bound efficiently to dcs and hep-2 cells. type 1 fimbriae-mediated binding to dcs ... | 2009 | 19383701 |
| multilocus variable-number tandem-repeat method for typing salmonella enterica serovar newport. | in recent years, the proportion of salmonella enterica infections represented by s. enterica serovar newport has increased markedly among humans and animals. multilocus variable-number tandem-repeat analysis (mlva) has proven to be useful in discriminating other highly clonal salmonella serovars. here, we report on the development of a highly discriminatory mlva for salmonella serovar newport. | 2009 | 19386855 |
| adhesion and growth inhibitory effect of chicken egg yolk antibody (igy) on salmonella enterica serovars enteritidis and typhimurium in vitro. | the protective effects of powder preparation of egg yolk immunoglobulin y (igy), specific to salmonella enteritidis and salmonella typhimurium outer membrane proteins (omp), against these two salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. the adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial caco-2 cell line. first, the conditions of salmonella adherence to caco-2 cells wer ... | 2009 | 19388827 |
| ferrous iron-binding protein omb of salmonella enterica serovar choleraesuis promotes resistance to hydrophobic antibiotics and contributes to its virulence. | salmonella enterica serovar choleraesuis (sc) is an important enteric pathogen that causes serious systemic infections in swine and humans. to identify the genes required for resistance to antimicrobial peptides, we constructed a bank of sc transposon mutants and screened them for hypersensitivity to the cationic peptide polymyxin b. here we report one isolated polymyxin b-susceptible mutant that also exhibited increased sensitivity toward human neutrophil peptide alpha-defensin 1 (hnp-1) and hy ... | 2009 | 19389759 |
| proteomic analysis of the adaptive response of salmonella enterica serovar typhimurium to growth under anaerobic conditions. | in order to survive in the host and initiate infection, salmonella enterica needs to undergo a transition between aerobic and anaerobic growth by modulating its central metabolic pathways. in this study, a comparative analysis of the proteome of s. enterica serovar typhimurium grown in the presence or absence of oxygen was performed. the most prominent changes in expression were measured in a semiquantitative manner using difference in-gel electrophoresis (dige) to reveal the main protein factor ... | 2009 | 19389776 |
| accurate prediction of secreted substrates and identification of a conserved putative secretion signal for type iii secretion systems. | the type iii secretion system is an essential component for virulence in many gram-negative bacteria. though components of the secretion system apparatus are conserved, its substrates--effector proteins--are not. we have used a novel computational approach to confidently identify new secreted effectors by integrating protein sequence-based features, including evolutionary measures such as the pattern of homologs in a range of other organisms, g+c content, amino acid composition, and the n-termin ... | 2009 | 19390620 |
| search for salmonella spp. in ostrich productive chain of brazilian southeast region. | we analyzed ostriches from an equipped farm located in the brazilian southeast region for the presence of salmonella spp. this bacterium was investigated in 80 samples of ostrich droppings, 90 eggs, 30 samples of feed and 30 samples of droppings from rodents. additionally, at slaughter-house this bacterium was investigated in droppings, caecal content, spleen, liver and carcasses from 90 slaughtered ostriches from the studied farm. also, blood serum of those animals were harvested and submitted ... | 2009 | 19390983 |