Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| structure of alcohol dehydrogenase from entamoeba histolytica. | the structure of the apo form of alcohol dehydrogenase from a single-cell eukaryotic source, entamoeba histolytica, has been determined at 1.8 a. to date, bacterial and archeal alcohol dehydrogenases, which are biologically active as tetramers, have crystallized with tetramers in the asymmetric unit. however, the current structure has one independent dimer per asymmetric unit and the full tetramer is generated by application of the crystallographic twofold symmetry element. this structure reveal ... | 2006 | 16627948 |
| entamoeba histolytica: ultrastructure of the chromosomes and the mitotic spindle. | we have analyzed by transmission electron microscopy the mitotic process of entamoeba histolytica trophozoites in an asynchronous population of axenically cultured parasites. our observations showed that nuclear microtubules, initially located at random in the karyosome during prophase, formed in subsequent stages a mitotic spindle closely related to the nuclear membrane at the polar regions of dividing nuclei. in late prophase and in anaphase, chromosomes appeared as dense bodies 0.1-0.5 microm ... | 2006 | 16631745 |
| entamoeba histolytica: inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites. | it has been demonstrated that expression of cyclooxygenase-2 (cox-2) isoform is induced by entamoeba histolytica in macrophages and polymorphonuclear cells during amoebic liver abscess (ala) formation in hamsters. trophozoites present in the lesion were also positive for cox-2 signal. however, no cross reactivity of the anti-cox-2 antibody with protein extract of cultivated trophozoites was found. to clarify if trophozoites are involved in pge(2) production during ala development, cox-2 expressi ... | 2006 | 16647055 |
| acetate kinase: not just a bacterial enzyme. | the bacterial enzymes acetate kinase (ak) and phosphotransacetylase (pta) form a key pathway for synthesis of the central metabolic intermediate acetyl coenzyme a (acetyl-coa) from acetate or for generation of atp from excess acetyl-coa. putative ak genes have now been identified in some eukaryotic microbes. in chlamydomonas reinhardtii and phytophthora species, ak forms a pathway with pta. ak has also been identified in non-yeast fungi but these fungi do not have pta. instead, ak forms a pathwa ... | 2006 | 16678422 |
| intestinal parasitic infections among school children of the dhahira region of oman. | to determine the prevalence of intestinal parasitic infections among primary school children in dhahira region of oman. | 2006 | 16680250 |
| unique posttranslational modifications of chitin-binding lectins of entamoeba invadens cyst walls. | entamoeba histolytica, which causes amebic dysentery and liver abscesses, is spread via chitin-walled cysts. the most abundant protein in the cyst wall of entamoeba invadens, a model for amebic encystation, is a lectin called eijacob1. eijacob1 has five tandemly arrayed, six-cys chitin-binding domains separated by low-complexity ser- and thr-rich spacers. e. histolytica also has numerous predicted jessie lectins and chitinases, which contain a single, n-terminal eight-cys chitin-binding domain. ... | 2006 | 16682461 |
| stress by heat shock induces massive down regulation of genes and allows differential allelic expression of the gal/galnac lectin in entamoeba histolytica. | gene expression analysis by microarray assay revealed that when exposed to stress, entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene transcription as well as differential allelic expression of key genes participating in virulence, such as the galactose/n-acetylgalactosamine (gal/galnac) lectin. | 2006 | 16682464 |
| impact of intestinal colonization and invasion on the entamoeba histolytica transcriptome. | a genome-wide transcriptional analysis of entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture. an affymetrix platform gene expression array was designed for this analysis that included probe sets for 9435 open reading frames (orfs) and 9066 5' and 3' flanking regions. transcripts were detected for > 80% of all orfs. a total of 523 transcripts (5.2% of all e. histolytica genes) were significantly changed in amebae isolated fro ... | 2006 | 16569449 |
| drug resistance in amoebiasis. | amoebiasis caused by entamoeba histolytica, is a major public health problem in developing countries. morphologically similar e. dispar is non pathogenic. because of the redefinition of e. histolytica and e. dispar, and the limited number of antiamoebic drugs available, a new approach to treat such individuals is necessary. the cost of treating asymptomatic individuals is highly exorbitant and not justifiable. the indiscriminate use of antiamoebic drugs can result in increased minimum inhibitory ... | 2006 | 16575108 |
| cloning and partial characterization of entamoeba histolytica ptpases. | reversible protein tyrosine phosphorylation is an essential signal transduction mechanism that regulates cell growth, differentiation, mobility, metabolism, and survival. two genes coding for protein tyrosine phophatases, designed ehptpa and ehptpb, were cloned from entamoeba histolytica. ehptpa and ehptpb proteins showed amino acid sequence identity of 37%, both ehptpases showed similarity with dictyostelium discoideum and vertebrate trasmembranal ptpases. mrna levels of ehptpa gene are up-regu ... | 2006 | 16513090 |
| entamoeba histolytica: computational identification of putative microrna candidates. | micrornas are an abundant class of approximately 20-24-nucleotide noncoding rnas that control the expression of several genes at the post-transcriptional level, regulating several important biochemical pathways. micrornas have been identified in many vertebrates including humans, and also in flies, nematodes, plants, and viruses. in the present work we have identified seventeen putative candidate micrornas in the pathogenic parasite entamoeba histolytica using a bioinformatic approach. putative ... | 2006 | 16515787 |
| synthesis, characterization and antiamoebic activity of 1-(thiazolo[4,5-b]quinoxaline-2-yl)-3-phenyl-2-pyrazoline derivatives. | a new series of 1-n-thiocarboxamide-3-phenyl-2-pyrazolines 1-6 was synthesized by cyclization of different mannich bases with unsubstituted thiosemicarbazide. the reaction of cyclized pyrazoline derivatives 1-6 with 2,3-dichloroquinoxaline afforded the title compounds 7-12. the structures of the new compounds were confirmed by elemental analyses as well as (1)h, (13)c nmr, ir and electronic spectral data. the hm1:imss strain of entamoeba histolytica parasite was cultured in vitro and the sensiti ... | 2006 | 16495051 |
| colonic amoebic abscess mimicking carcinoma of the colon. | amoebiasis is an uncommon disease in developed countries. its clinical presentation can be variable and non-specific, and the diagnosis can be easily overlooked. we report a case of colonic amoebic abscess mimicking advanced colonic cancer with acute intestinal obstruction and liver metastasis. the presentation, diagnosis, and treatment of amoebiasis are also reviewed. | 2006 | 16495594 |
| pleiotropic phenotype in entamoeba histolytica overexpressing dna methyltransferase (ehmeth). | the protozoan parasite entamoeba histolytica expresses a cytosine-5 dna methyltransferase (ehmeth) that belongs to the dnmt2 proteins family. the biological function of members of the dnmt2 family is unknown. constitutive overexpression of ehmeth resulted in a pleiotropic phenotype that includes accumulation of multinucleated cells, upregulation of heat shock protein 70 (hsp70) expression and resistance to oxidative stress. this pleiotropic phenotype suggests that ehmeth plays an important role ... | 2006 | 16497397 |
| epidemiology, etiology and pathophysiology of traveler's diarrhea. | traveler's diarrhea (td) is the most frequent health problem in travelers to developing countries. several personal and environmental risk factors are at the basis of td acquisition and are discussed in this paper. td is caused by a wide range of infectious organisms, etec and eaec bacteria strains being the main enteropathogens incriminated in td. other causative bacteria are: shigella spp., campylobacter spp., vibrio spp., aeromonas spp., salmonella spp., and plesiomonas spp. parasite species ... | 2006 | 16498258 |
| entamoeba histolytica up-regulates the cdc48-like protein, an aaa family member, during the activation of trophozoites with collagen type i and calcium. | 2006 | 16364463 | |
| sexual dimorphism in the control of amebic liver abscess in a mouse model of disease. | amebic liver abscess (ala) is the most common extraintestinal manifestation of human infection by the enteric protozoan parasite entamoeba histolytica. in contrast to intestinal infection, ala greatly predominates in males but is rare in females. since humans are the only relevant host for e. histolytica, experimental studies concerning this sexual dimorphism have been hampered by the lack of a suitable animal model. by serial liver passage of cultured e. histolytica trophozoites in gerbils and ... | 2006 | 16368964 |
| identification of differentially expressed genes in virulent and nonvirulent entamoeba species: potential implications for amebic pathogenesis. | entamoeba histolytica is a protozoan parasite that causes colitis and liver abscesses. several entamoeba species and strains with differing levels of virulence have been identified. e. histolytica hm-1:imss is a virulent strain, e. histolytica rahman is a nonvirulent strain, and entamoeba dispar is a nonvirulent species. we used an e. histolytica dna microarray consisting of 2,110 genes to assess the transcriptional differences between these species/strains with the goal of identifying genes who ... | 2006 | 16368989 |
| cpg-oligodeoxynucleotide is a potent adjuvant with an entamoeba histolytica gal-inhibitable lectin vaccine against amoebic liver abscess in gerbils. | the protozoan parasite entamoeba histolytica causes invasive amoebiasis characterized by amoebic dysentery and liver abscesses (ala). the e. histolytica galactose/n-acetyl-d-galactosamine-inhibitable lectin (gal-lectin), an immunogenic surface molecule involved in colonization and invasion, is a promising vaccine candidate against amoebiasis. gal-lectin is known to induce th1 cytokines in macrophages and spleen cells in vitro, and a th1 response is thought to be protective against ala. in this s ... | 2006 | 16369009 |
| cytokine expression in cd4(+) cells exposed to the monocyte locomotion inhibitory factor produced by entamoeba histolytica. | entamoeba histolytica produces monocyte locomotion inhibitory factor (mlif), a pentapeptide with in vitro and in vivo anti-inflammatory properties. mlif may interfere with leukocyte migration, disturbing the balance of pro- and anti-inflammatory cytokines secreted by cd4(+) t lymphocytes. we evaluated the effect of mlif on expression of pro- and anti-inflammatory cytokines in human cd4(+) t lymphocytes. regulatory cytokines [interleukin-1 beta (il-1beta), il-2, interferon gamma (ifn-gamma), il-5 ... | 2006 | 16374615 |
| membrane trafficking as a virulence mechanism of the enteric protozoan parasite entamoeba histolytica. | 2006 | 16374616 | |
| pathogenesis of acute experimental liver amebiasis. | classical descriptions of the pathology of amebiasis portray the parasite as the cause of tissue damage and destruction, and in recent years a number of amebic molecules have been identified as virulence factors. in this review we describe a series of experiments that suggest a more complex host-parasite relation, at least during the early stages of acute experimental amebic liver abscess in hamsters. the problems of extrapolating experiments in vitro to explain observations in vivo are discusse ... | 2006 | 16380320 |
| experimental amebiasis: a selected review of some in vivo models. | the use of in vivo animal models in amebiasis has contributed significantly to the knowledge of this common human parasitic disease. although there is no animal model that mimics the whole cycle of the human disease, the use of different susceptible and resistant laboratory animals and the availability for many years of techniques for the axenic culture of trophozoites of entamoeba histolytica have allowed a better understanding of the parasite and the host-parasite relationship. the recent intr ... | 2006 | 16380321 |
| calcium-binding proteins of entamoeba histolytica. | calcium plays an essential role in many fundamental processes in almost all eukaryotic cells including protozoan parasite entamoeba histolytica. many of the calcium-mediated processes are carried out through the help of calcium-binding proteins (cabps). a few of these e. histolytica cabps have been described before. these proteins are unique to this organism and are thought to be essential. availability of genome sequence has opened up the possibility of studying cabps at the whole genome level. ... | 2006 | 16380322 |
| epigenetic silencing of gene expression in entamoeba histolytica. | transcriptional silencing of an amebapore (ap-a) gene occurred in entamoeba histolytica following the transfection of plasmids containing a dna segment (473 bp) homologous to the 5' upstream region of the gene. this segment contains the promoter region of the ap-a gene, a t-rich stretch, followed by a truncated sine1 (short interspersed element) that is transcribed from the opposite strand. the downstream silencing of the ap-a gene did not occur with plasmids containing the entire sine1 sequence ... | 2006 | 16380323 |
| the cytoskeleton of entamoeba histolytica: structure, function, and regulation by signaling pathways. | pathogenesis in the parasite entamoeba histolytica has been related to motility of the trophozoites. motility is an important feature in amebas as they perform multiple motile functions during invasion of host tissues. as motility depends on the organization and regulation of the cytoskeleton elements, in particular of the actin cytoskeleton, the study of the molecular components of the machinery responsible for movement has been a key aspect to study in this parasite. although many of the compo ... | 2006 | 16380324 |
| new insights into molecular mechanisms of phagocytosis in entamoeba histolytica by proteomic analysis. | the protozoan parasite entamoeba histolytica ingests microorganisms and mammalian cells. phagocytosis is essential for cell growth and is implicated in pathogenesis of e. histolytica. phagocytosis consists of a number of steps including recognition of and binding to ligands on the target cells via a galactose/n-acetylgalactosamine-specific lectin, activation of a signaling pathway leading to cytoskeletal reorganization, and vesicle trafficking, all of which play distinct but coordinated roles in ... | 2006 | 16380325 |
| host tissue invasion by entamoeba histolytica is powered by motility and phagocytosis. | during amebiasis, e. histolytica motility is a key factor to achieve its progression across tissues. the pathogenicity of e. histolytica includes its capacity to phagocyte human cells. motility requires polarization of e. histolytica that involves protrusion of a pseudopod containing actin and associated proteins [myosin ib, abp-120 and a p21-activated kinase (pak)] and whole-cell propulsion after contraction of the rear of the cell, where myosin ii and f-actin are concentrated. an interesting c ... | 2006 | 16380326 |
| methods for the investigation of diversity in entamoeba histolytica. | the ability to distinguish variants of a species has many potential applications. in entamoeba histolytica the first method to detect variation was based on isoenzyme analysis. however, this approach has been superseded by dna-based analysis. in this review i discuss the basis of the variation detected in e. histolytica by the various molecular methods that have been published to date. information on diversity in other species is mentioned where such information exists. | 2006 | 16380327 |
| prevalence and incidence of entamoeba histolytica infection in south africa and egypt. | there are little data on the true prevalence and incidence of entamoeba histolytica infection in africa. this is due to the inability, historically, to differentiate entamoeba histolytica from the more common, but non-pathogenic, entamoeba dispar. in addition, newer studies have demonstrated that the previous gold standard, culture with zymodeme analysis, is insensitive in detecting the presence of infection, especially when compared to pcr. recent published articles as well as data from the aut ... | 2006 | 16380329 |
| epidemiology and treatment of amebiasis in hué, vietnam. | hué, a city of about 300,000 inhabitants in central vietnam, is known as a "hot spot" for diseases caused by entamoeba histolytica and in particular for amebic liver abscess (ala), thus representing an excellent site for studies on the epidemiology and treatment of e. histolytica infections. accordingly, during the last few years, various studies on this topic have been performed in hué in close collaboration among the hué medical college, the hué central hospital and the bernhard nocht institut ... | 2006 | 16380330 |
| diversity of clinical isolates of entamoeba histolytica in japan. | in japan, amebiasis is domestically transmitted by two major populations: male homosexuals and mentally handicapped persons, which is remarkably different from most other developed countries where entamoeba dispar infection is predominantly observed. here we briefly summarize epidemiology of amebiasis in japan. we also review our current understanding of the diversity of entamoeba histolytica clinical isolates in japan, based on polymorphic genetic markers, clinical representations, and in vivo ... | 2006 | 16380332 |
| adherence-blocking vaccine for amebiasis. | the gal/galnac lectin is a candidate vaccine antigen for an amebiasis vaccine due to its mediation of parasite adherence to the human intestine, because partial immunity in humans is associated with a mucosal iga response against it, and because it is effective as a vaccine against amebic colitis in the murine model. the leca domain of the gal/galnac lectin contains neutralizing antibody epitopes. leca contains the active site of the lectin (the carbohydrate recognition domain or "crd") and has ... | 2006 | 16380334 |
| the current status of an amebiasis vaccine. | efficient control of infectious diseases requires the development and application of suitable vaccines. development of vaccines against amebiasis is still in its infancy. however, in recent years progress has been made in the identification of possible vaccine candidates, the route of application and the understanding of the immune response that is required for protection against amebiasis. | 2006 | 16380335 |
| genomic and proteomic approaches highlight phagocytosis of living and apoptotic human cells by the parasite entamoeba histolytica. | phagocytosis plays a major role during the invasive process of the human intestine by the pathogenic amoeba e. histolytica. this parasite is the etiologic agent causing amoebic dysentery, a worldwide disease causing 50 million of clinical cases leading to about 100,000 deaths annually. the invasive process is characterized by a local acute inflammation and the destruction of the intestinal tissue at the invasion site. the recent sequencing of the e. histolytica genome has opened the way to large ... | 2006 | 16386742 |
| entamoeba histolytica: fyve-finger domains, phosphatidylinositol 3-phosphate biosensors, associate with phagosomes but not fluid filled endosomes. | endocytosis is an important virulence function for entamoeba histolytica, the causative agent of amoebic dysentery. although a number of e. histolytica proteins that regulate this process have been identified, less is known about the role of lipids. in other systems, phosphatidylinositol 3-phosphate (pi3p), a product of phosphatidylinositol 3-kinase (pi 3-kinase), has been shown to be required for endocytosis. fyve-finger domains are protein motifs that bind specifically to pi3p. using a pi3p bi ... | 2006 | 16387299 |
| heterologous expression of an entamoeba histolytica chitin synthase in saccharomyces cerevisiae. | chitin in the cyst wall of entamoeba histolytica is made by two chitin synthases (chs), one of which is unique (ehchs-1) and one of which resembles those of insects and nematodes (ehchs-2). ehchs-1 is deposited chitin in the lateral wall of transformed saccharomyces cerevisiae chs mutants, independent of accessory proteins (chs4p to chs7p) required by yeast chs3p. | 2006 | 16400183 |
| dioxo- and oxovanadium(v) complexes of thiohydrazone ons donor ligands: synthesis, characterization, reactivity, and antiamoebic activity. | as a contribution to the development of novel vanadium complexes with pharmacologically interesting properties, two neutral dioxovanadium(v) complexes [vo2(hpydx-sbdt)] (1) and [vo2(hpydx-smdt)] (3) [h2pydx-sbdt (i) and h2pydx-smdt (ii) are the schiff bases derived from pyridoxal and s-benzyl- or s-methyldithiocarbazate] have been synthesized by the reaction of [vo(acac)2] and the potassium salts of the ligands in methanol followed by aerial oxidation. heating of the methanolic solutions of thes ... | 2006 | 16441138 |
| kikuchi fujimoto disease secondary to entamoeba histolytica: case report. | kikuchi fujimoto disease (kfd) is a rare histiocytic necrotizing lymphadenitis which has a benign self-limiting clinical course. its origin is unknown, but an abnormal autoimmune reaction has been suggested and infection is often considered to be an inciting agent. a 50-year-old man presented with fever, malaise, fatigue and sweat of 7 days duration, and diarrhea for 2 days. physical examination revealed five mobile and painless cervical adenopathies. entamoeba histolytica trophozoites and cysts ... | 2006 | 16442163 |
| identification and characterization of ehabc a1, an entamoeba histolytica group a abc transporter with similarity to ced-7. | 2006 | 16442643 | |
| the anti-amoebic activity of some medicinal plants used by aids patients in southern thailand. | the anti-amoebic activities of chloroform, methanol and water extracts from 12 thai medicinal plants (39 extracts) commonly used by aids patients in southern thailand were screened, at a concentration of 1,000 microg/ml, against entamoeba histolytica strain hth-56:mutm and strain hm1:imss growing in vitro. the extracts were incubated with 2x10(5) e. histolytica trophozoites/ml of medium at 37 degrees c under anaerobic conditions for 24 h. the cultures were examined with an inverted microscope an ... | 2006 | 16447069 |
| rho signaling in entamoeba histolytica modulates actomyosin-dependent activities stimulated during invasive behavior. | interaction of entamoeba histolytica trophozoites with target cells and substrates activates signaling pathways in the parasite. phosphorylation cascades triggered by phospho-inositide and adenyl-cyclase-dependent pathways modulate reorganization of the actin cytoskeleton to form structures that facilitate adhesion. in contrast, little is known about participation of rho proteins and rho signaling in actin rearrangements. we report here the in vivo expression of at least one rho protein in troph ... | 2006 | 16421927 |
| entamoeba histolytica infection in children and protection from subsequent amebiasis. | the contribution of amebiasis to the burden of diarrheal disease in children and the degree to which immunity is acquired from natural infection were assessed in a 4-year prospective observational study of 289 preschool children in an urban slum in dhaka, bangladesh. entamoeba histolytica infection was detected at least once in 80%, and repeat infection in 53%, of the children who completed 4 years of observation. annually there were 0.09 episodes/child of e. histolytica-associated diarrhea and ... | 2006 | 16428733 |
| human tumor necrosis factor is a chemoattractant for the parasite entamoeba histolytica. | in an analysis of the molecular factors triggering amebiasis, we investigated the chemotaxis of entamoeba histolytica toward tumor necrosis factor (tnf) in vitro, using quantitative imaging techniques. our findings enabled us to propose a hitherto unknown role for tnf as a chemokinetic and chemoattractant agent for this parasite. | 2006 | 16428794 |
| identification and functional characterization of ehclc-a, an entamoeba histolytica clc chloride channel located at plasma membrane. | clc chloride channels perform a wide variety of physiological functions and they had been characterized in animals, yeast, plants and bacteria but not in protozoa. by blast search we found in entamoeba histolytica, the protozoan responsible for human amoebiasis, two genes (ehclc-a and ehclc-b) encoding for putative polypeptides with 25-30% identity to clc chloride channels of several organisms. reverse transcription polymerase chain reaction (rt-pcr) experiments showed that both genes are transc ... | 2006 | 16430698 |
| could the homologous sequence of anti-inflammatory pentapeptide (mlif) produced by entamoeba histolytica in the n protein of rabies virus affect the inflammatory process? | amebiasis and rabies are public health problems, and they have in common a poor inflammatory effect in the target organs that they affect. in the genbank, it was found that the anti-inflammatory peptide monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica homologates 80%, with a fragment of the n protein of the rabies virus. we speculated if the n protein could contribute to the scant inflammatory reaction produced by rabies virus in central nervous system. the n protei ... | 2006 | 16341883 |
| characterization of protein geranylgeranyltransferase i from the enteric protist entamoeba histolytica. | entamoeba histolytica is a unique protozoan parasite possessing both protein farnesyltransferase and geranylgeranyltrasferase i (ggt-i) for isoprenylation of small gtpases. in this study, we demonstrated unique enzymological properties of the amebic ggt-i (ehggt-i), including substrate specificity and insensitivity to known mammalian inhibitors. some of important residues of the catalytic beta subunit implicated in the specificity for gtpase acceptors and prenyl donors are substituted in ehggt-i ... | 2006 | 16300841 |
| synthesis and antiamoebic activity of new 1-n-substituted thiocarbamoyl-3,5-diphenyl-2-pyrazoline derivatives and their pd(ii) complexes. | some 1-n-substituted thiocarbamoyl-3,5-diphenyl-2-pyrazoline derivatives (l), 1-8 were synthesized by a base-catalyzed claisen-schmidt condensation of benzaldehyde with acetophenone followed by cyclization with various n-4 substituted thiosemicarbazides. the palladium(ii) complexes [pdlcl2], 1a-8a of these ligands were obtained by reacting them with [pd(dmso)2cl2]. all compounds have been characterized by means of elemental analyses, electronic, ir, 1h nmr and mass spectroscopic data, while the ... | 2006 | 16300860 |
| unique organisation of trna genes in entamoeba histolytica. | the genome sequence of the protistan parasite entamoeba histolytica hm-1:imss has been completed recently. among the findings has been a unique organisation for the trna genes in this organism. forty-two of the trna isoacceptor types are encoded in tandem arrays that vary in unit length from 490 to 1775 basepairs and contain from 1 to 5 trna genes. in three cases a 5s rna gene is also present in the unit. an estimated 10% of the genome is made up of these arrays. interspersed between rna-encodin ... | 2006 | 16307803 |
| identification of a family of bspa like surface proteins of entamoeba histolytica with novel leucine rich repeats. | 2006 | 16199101 | |
| characterization of entamoeba histolytica alpha-actinin. | we have cloned, expressed and characterized a alpha-actinin-like protein of entamoeba histolytica. analysis of the primary structure reveals that the essential domains of the alpha-actinin protein family are conserved: an n-terminus actin-binding domain, a c-terminus calcium-binding domain and a central helical rod domain. however, the rod domain of this entamoeba protein is considerably shorter than the rod domain in alpha-actinins of higher organisms. the cloned entamoeba 63 kda protein is rec ... | 2006 | 16219372 |
| entamoeba histolytica sequences and their relationship with experimental liver abscesses in hamsters. | the purpose of the present paper was to analyse the association between sequences of entamoeba histolytica and their relationship with the development of hepatic abscesses in hamsters, using a complementary dna library for e. histolytica. from the sequences obtained, we designed oligonucleotides for amplification by pcr. trophozoites were isolated from faeces of 11 patients in whom cysts from e. histolytica were identified, and these trophozoites were then subjected to monoaxenic culture. then 1 ... | 2006 | 16283410 |
| prevalence and genetic diversity of entamoeba histolytica in an institution for the mentally retarded in the philippines. | a total of 113 mentally retarded patients residing in a mental institution in metropolitan manila, philippines, were screened for the presence of entamoeba histolytica based on microscopy and polymerase chain reaction (pcr). anti-e. histolytica antibodies were also screened in 97 serum samples collected using immunofluorescence antibody (ifa) test. parasitological examination showed e. histolytica/entamoeba dispar in 43 cases (38.05%), while pcr detected 74 cases (65.48%) positive for e. histoly ... | 2006 | 16284779 |
| biochemical and functional characterization of phosphoserine aminotransferase from entamoeba histolytica, which possesses both phosphorylated and non-phosphorylated serine metabolic pathways. | the enteric protozoan parasite entamoeba histolytica is a unicellular eukaryote that possesses both phosphorylated and non-phosphorylated serine metabolic pathways. in the present study, we described enzymological and functional characterization of phosphoserine aminotransferase (psat) from e. histolytica. e. histolytica psat (ehpsat) showed maximum activity for the forward reaction at basic ph, dissimilar to mammalian psat, which showed sharp neutral optimum ph. ehpsat activity was significantl ... | 2006 | 16289358 |
| kinetics and strain variation of phagosome proteins of entamoeba histolytica by proteomic analysis. | the protozoan parasite entamoeba histolytica ingests and feeds on microorganisms and mammalian cells. phagocytosis is essential for cell growth and implicated in pathogenesis of e. histolytica. we report here the dynamic changes of phagosome proteins during phagosome maturation by proteomic analysis using reversed-phase capillary liquid chromatography and ion trap tandem mass spectrometry. phagosomes were isolated at various intervals after internalization of latex beads. immunoblot analysis and ... | 2006 | 16290089 |
| dehydroepiandrosterone decreases while cortisol increases in vitro growth and viability of entamoeba histolytica. | in vitro exposure of entamoeba histolytica trophozoites to the sex steroids 17beta-estradiol, progesterone, and dehydrotestosterone had little effect on parasite viability or proliferation. however, treatment with the adrenal steroid dehydroepiandrosterone (dhea) markedly inhibited parasite proliferation, adherence and motility, and at a certain dose it induced trophozoite lysis. the opposite effect on proliferation was found when the trophozoites were exposed to cortisol. moreover, dhea decreas ... | 2006 | 16293437 |
| entamoeba histolytica and entamoeba dispar: comparison of two pcr assays for diagnosis in a non-endemic setting. | detection of entamoeba histolytica, the causative agent of amoebiasis, is an important goal of the clinical parasitology laboratory. the identification of entamoeba dispar as a morphologically identical but non-pathogenic species has highlighted the need for non-microscopic detection methods able to differentiate between the two organisms. in this study we evaluated the utility of conventional pcr and real-time pcr as methods for identification and differentiation of e. histolytica and e. dispar ... | 2006 | 16274714 |
| cytokine and chemokine responses in patients co-infected with entamoeba histolytica/dispar, necator americanus and mansonella perstans and changes after anti-parasite treatment. | this study examined the impact of concurrent parasite infections (amoebiasis, filariasis, necatoriasis) and the effect of anti-parasite treatment on cytokine and chemokine responses in singly and poly-parasitized patients. cellular reactivity and parasite-specific th1- and th2-type cytokine and chemokine profiles were investigated before and six weeks after treatment. in those patients infected with three parasite species, cellular secretion of interleukin 5 (il-5) and il-12p40 by pbmc was stron ... | 2006 | 16239120 |
| glycosylated inositol phospholipid from entamoeba histolytica: identification and structural characterization. | 2006 | 16242191 | |
| hemolytic uremic syndrome associated with entamoeba histolytica intestinal infection. | amebiasis secondary to entamoeba histolytica (e. histolytica) continues to be a major source of morbidity and mortality worldwide, mainly in developing countries. an association between amebic dysentery and hemolytic uremic syndrome (hus) has been mentioned, but a cause-and-effect relationship has never been confirmed. we report the case of an 11-year-old healthy white female who developed severe bloody diarrhea after drinking contaminated unboiled water, developing classic hus and requiring acu ... | 2006 | 16222550 |
| intestinal parasitosis among the elderly people in kathmandu valley. | present study was carried out among the elderly people (60+ years of age) from august 2005 to july 2006 in kathmandu valley to assess the prevalence of intestinal parasitosis in them. stool samples were collected from 235 elderly people (122 from government elderly home, 66 from private elderly home and 47 from the households in a rural community). the samples were examined by formal ether sedimentation and sheather's sucrose floatation followed by kinyoun's modified ziehl-neelsen staining. the ... | 2006 | 17357641 |
| [the process of n-glycosylation of proteins in entamoeba histolytica]. | 2006 | 17578075 | |
| [study on genetic approach in the detection of cryptosporidium parvum and giardia lamblia in acquired immunodeficiency syndrome patients]. | to establish genetic method in detecting cryptosporidium parvum and giardia lamblia which often coinfected with aids patients. | 2006 | 17343184 |
| [investigation of intestinal parasites in residents of the kayseri karpuzsekisi basin]. | in this study, we collected stool and cellophane tape samples from residents in four villages located in the kayseri-karpuzsekisi basin in order to investigate the distribution of intestinal parasites. a total of 240 samples collected from hanyeri (40), sariküklü (20), dokuzpinar (80) and karpuzsekisi (120) were investigated. the parasites detected were as follows: blastocystis hominis, 82 (34.16%); enterobius vermicularis, 37 (15.41%); entamoeba coli 26, (10.83%); entamoeba hartmanni, 10 (4.16% ... | 2006 | 17160846 |
| [incidence of intestinal parasites in municipal sanitary workers in malatya]. | the incidence of intestinal parasites is closely related to such factors as the socio-economic level of the society, nutritional and hygienic habits, climate, environmental conditions, infrastructure and degree of literacy. in this study, the municipal sanitary workers who are regarded as a high risk group in malatya were examined for intestinal parasites. cellophane slides and fecal samples from 241 workers were examined and intestinal parasites were found in 93 (39.0%). the most common parasit ... | 2006 | 17160847 |
| cryptosporidiosis and other intestinal protozoan infections in children less than one year of age in mexico city. | parasitic diseases are very important in mexico because of their economic impact and adverse effects on normal growth in children. cryptosporidiosis has been associated with acute diarrhea in immune competent and incompetent human hosts, fecal contamination of drinking water sources, and handling of animals. due to the lack of reports on cryptosporidiosis in mexico, we conducted a parasitologic study in children with diarrhea and other clinical symptoms. the main objectives were 1) to determine ... | 2006 | 17172373 |
| comparison of the proteome profiles of entamoeba histolytica and its close but non-pathogenic relative entamoeba dispar. | the microaerophilic protist entamoeba histolytica is a source of considerable morbidity and mortality in many countries of the world by acting as a causative agent of amoebic dysentery and liver abscess. its close relative, entamoeba dispar, also colonises the human colon but is non-pathogenic. in the present study, we wanted to assess if the close relatedness of these two organisms could be used to identify virulence factors in e. histolytica through a comparison of the protein expression profi ... | 2006 | 17131239 |
| entamoeba histolytica cell movement: a central role for self-generated chemokines and chemorepellents. | entamoeba histolytica cells, the cause of amoebic dysentery, are highly motile, and this motility is an essential feature of the pathogenesis and morbidity of amoebiasis. however, the control of e. histolytica motility within the gut and during invasion is poorly understood. we have used an improved chemotaxis assay to identify the key extracellular signals mediating entamoeba chemotaxis. the dominant responses we observe are caused by factors generated by e. histolytica cells themselves. medium ... | 2006 | 17132728 |
| locally acquired infection with entamoeba histolytica in men who have sex with men in australia. | 2006 | 17137428 | |
| oral fecal parasites and personal hygiene of food handlers in abeokuta, nigeria. | ingestion of infective eggs and cysts of faecal orally transmissible parasites has been linked with the level of environmental and personal hygiene. the possibility of contamination of food with eggs and cysts by infected food vendors has also been recorded. | 2006 | 17140338 |
| decreased chemiluminescence in leukocyte adhesion deficiency presenting with recurrent sepsis, amoebiasis and candida albicans urinary tract infection. | leukocyte adhesion deficiency (lad) is a rare disorder of cellular immunity, generally due to various mutations producing reduced or altered expression of membrane integrins. the authors report a case of lad due to integrins expression imbalance. lad was suspected after recurrent sepsis, fungal infection and amoebiasis with persistent leukocytosis. neutrophils were studied with chemiluminescence showing decreased functional activity: up to now, this seems the first chemiluminescence study of neu ... | 2006 | 17146424 |
| intestinal parasites in dakahlia governorate, with different techniques in diagnosing protozoa. | a total of 3180 patients attending mansoura university hospitals' clinics, were subjected to stool examination by direct wet smear, formol-ether concentration, original formol-tween concentration, modified formol-tween concentration, modified sheather's sugar floatation, potassium hydroxide concentration and gomori's trichrome stain, and modified kinyoun's acid-fast stain, and ryan's trichrome blue stain for microsporidia. the intestinal helminthes in a descending order of abundance were: s. man ... | 2006 | 17153711 |
| parasitic etiology of childhood diarrhea. | the objective was to determine the prevalence of parasitic agents among under-five children with diarrhea in ilesa, nigeria and the clinical correlates of diarrhea associated with parasitic infestation. all under-five children presenting with diarrhea in the hospital had stool microscopic examination. children with parasites in diarrheic stools (cases) were compared with those without (controls) for clinical features. out of 300 under-five children with diarrhea, 70 (23.3%) had parasites. there ... | 2006 | 17202634 |
| vaccines for amoebiasis: barriers and opportunities. | amoebiasis, infection by the protozoan parasite entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. while improved sanitation could lead to the eradication of this disease, it is unlikely that this will occur worldwide in the foreseeable future; thus alternative measures must be pursued. one approach is to develop a vaccine to prevent this deadly disease. clinical studies indicate that mucosal immunity may provide some protection against recur ... | 2006 | 17274850 |
| invasive amebiasis. | digestive amoebiasis with his invasive form is an unusual pathology encountered in the temperate zone. this could lead to a life threatening complication: systemic amoebiasis. a 55-year-old male was treated successfully of systemic amoebiasis in a third referral hospital. the diagnosis was established based on epidemiology data and microscopical identification of trophozoites of entamoeba histolytica. the amoebicidal, antibiotic and supportive treatments was firstly administrated. the clinical p ... | 2006 | 17278650 |
| parasitism in kansas in the 1800s: a glimpse to the past through the analysis of grave sediments from meadowlark cemetery. | during the excavations of the xix century meadowlark cemetery (manhattan, kansas, us), samples of sediments were taken from around five skeletons, and analyzed to detect intestinal parasites. no helminth eggs were found, but immunological elisa tests for entamoeba histolytica were positive in three samples. the immunological techniques have been successfully used in paleoparasitology to detect protozoan infections. amoebiasis could have been a severe disease in the past, especially where poor sa ... | 2006 | 17308809 |
| [the distribution of patients with intestinal parasites presenting at the parasitology laboratory of the mustafa kemal university medical faculty]. | in this study, fecal specimens of 3,679 patients presenting at the parasitology laboratory of the medical faculty of the mustafa kemal university between september and may 2005 were examined for intestinal parasites. a total 3,679 stool specimens from 1,260 (34.3%) males and 2,419 (65.7%) females for intestinal parasites using the native-lugol method were examined. intestinal parasites were found in 265 (34.23%) male and 509 (65.76%) females. one or more intestinal parasites were detected in 774 ... | 2006 | 17309033 |
| [comparison of the results of examination of fecal samples from students at six months intervals in the alahaci village primary school in sivas]. | the aim of this study was to determine the distribution of intestinal parasites in primary school children in the alahaci central village in the sivas province, but owing to high prevalence of parasites in soil, new stool specimens were taken from the same subject group after 6 months. in the first stage of investigation, stool samples from a total of 189 children were taken and examined. it was found that the 110 (58.2%) out of 189 subjects were infected with parasites. the distribution of para ... | 2006 | 17309034 |
| [the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2003 and 2004]. | a retrospective evaluation of the data from 7,712 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2003 and december 2004 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome staining method after sedimentation by the fecal concentration tube. one or more parasites were detected in 495 (6.41%) of the patients. the distribution of the intestinal ... | 2006 | 17309035 |
| prevalence of intestinal parasites in the area of parma during the year 2005. | intestinal parasitosis represent a relevant clinical problem, especially in developing countries, where they are responsible for morbidity and mortality in adults and children and many epidemiological data are available for these areas. the actual situation of intestinal parasitosis in europe is not yet well investigated since they are usually not notified. we describe the occurrence of intestinal parasitosis in our laboratory from january to december 2005. | 2006 | 17312984 |
| frequency of giardia lamblia among children in dohuk, northern iraq. | out of 1261 stool specimens collected from children in dohuk city, northern iraq, the prevalence of giardia lamblia infection was 38.5%. the highest rate of infection was in orphan care centres (48.1%) and the lowest in the paediatric hospital (31.3%). the age group 10-12 years had the highest rate (81.2%) and 7-9 years the lowest (22.9%); boys had a higher rate than girls. some infected samples (70/486) showed double or triple infections and g. lamblia was combined with hymenolepis nana, blasto ... | 2006 | 17333793 |
| [advances in research on virulence factors of entamoeba histolytica]. | the parasitic protozoan entamoeba histolytica is an important pathogen responsible for intestinal and extraintestinal amebiasis in human, claiming up to 100 000 victims every year. this parasite is characterized by its high potential for invading and destroying human tissues. invasion past the mucous barrier of the colonic epithelium leads to the development of colitis and amebic dysentery. dissemination of the trophozoite through blood stream permits the invasion of other tissues, resulting in ... | 2006 | 17366980 |
| [diagnosis and treatment of acute amebian liver abscess with laparoscopic method]. | laparoscopic surgical treatment was performed at 55 patients with acute amebian liver abscess. the diagnosis of acute amebian liver abscess was verified with clinical, serological, echoscopic, x-ray and ct methods. the laparoscopic method permits to sanify the abscess cavity of any localization. all the 55 patients have been discharged in good health. laparoscopic method is maximally rational, associated with minimal surgical trauma, permits to decrease hospital stay and period of postoperative ... | 2006 | 17419485 |
| [hepatic amebiasis]. | 2006 | 17465190 | |
| infection by human immunodeficiency virus-1 is not a risk factor for amebiasis. | 2006 | 17123964 | |
| changes in bacterial profile during amebiasis: demonstration of anaerobic bacteria in ala pus samples. | little is known about the changes in gut resident flora during amebic colitis and amebic liver abscess (ala) caused by entamoeba histolytica infection. fecal samples from ala patients, from healthy e. histolytica negative and positive (asymptomatic) individuals, and from pre- and post-metronidazole-treated healthy volunteers and pus samples from ala patients were tested for the presence of various bacterial genera using 16s rrna-based primers. statistically significant reduction in lactobacillus ... | 2006 | 17123981 |
| [parasitic fauna and the frequency of entamoeba histolytica/entamoeba dispar detected by elisa in stool samples in sanliurfa, turkey]. | amoebiasis is a significant health problem in developing countries. humans are infected by two morphologically identical species of entamoeba. entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. the aim of this study was to determine the prevalence of the e. histolytica/e. dispar complex using the elisa method on stools of patients. a total of 1600 stool specimens were examined using lugol preparations and the modified ritchie method. a total of 58 ... | 2006 | 17124656 |
| interaction of vibrio cholerae o139 with an intestinal parasite, entamoeba histolytica. | 2006 | 17108284 | |
| [intestinal parasitosis in french west indies: endemic evolution from 1991 to 2003 in the university hospital of pointe-a-pitre, guadeloupe]. | guadeloupe is one of the french west indies, where, until 1960, intestinal parasitic infections were endemic. in the microbiological laboratory of the university hospital of the island, we carried out a retrospective study of the 17,660 stool examinations received from 1991 to 2003. all the stool specimens were examined using at least a wet mount preparation and a concentration method (bailenger). specific techniques such as ziehl-neelsen modified acid fast stain, chromotrope staining procedure ... | 2006 | 17111973 |
| mass treatment for intestinal helminthiasis control in an amazonian endemic area in brazil. | the objective of the present study was to estimate the prevalence of soil-transmitted helminthiasis and evaluate the sanitary conditions and the role of a mass treatment campaign for control of these infections in santa isabel do rio negro. a cross-sectional survey was carried out in 2002, to obtain data related to the sanitary conditions of the population and fecal samples for parasitological examination in 308 individuals, followed by a mass treatment with albendazole or mebendazole with cover ... | 2006 | 17119673 |
| [occurrence of entamoeba histolytica/entamoeba dispar in ambulatory patients of recife, pe]. | the objective this study was to determine the occurrence of the species entamoeba histolytica/entamoeba díspar in clinical samples of ambulatory patients in pernambuco. a specific assay for entamoeba histolytica was used in this study, which identified no positive fecal samples among the 213 patients. these results confirm that e. dispar is the dominant species in pernambuco state. | 2006 | 17119758 |
| pathogenicity of entamoeba dispar under xenic and monoxenic cultivation compared to a virulent e. histolytica. | two xenic isolates and cloned cultures of entamoeba dispar were submitted to monoxenization using crithidia fasciculata as the associated organism. growth in monoxenic cultivation and ability of xenic and monoxenic trophozoites to destroy vero cells and produce lesions in hamster livers were compared to those of a virulent e. histolytica. parental and cloned e. dispar under monoxenic cultivation showed a remarkable lower growth than the monoxenic e. histolytica and were avirulent in both in vivo ... | 2006 | 17086310 |
| expression, purification and crystallization of l-methionine gamma-lyase 2 from entamoeba histolytica. | l-methionine gamma-lyase (mgl) is considered to be an attractive target for rational drug development because the enzyme is absent in mammalian hosts. to enable structure-based design of drugs targeting mgl, one of the two mgl isoenzymes (ehmgl2) was crystallized in the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters a = 88.89, b = 102.68, c = 169.87 a. the crystal diffracted to a resolution of 2.0 a. the presence of a tetramer in the asymmetric unit (4 x 43.1 kda) gives a matt ... | 2006 | 17012806 |
| multidrug resistance in amoebiasis patients. | amoebiasis, caused by entamoeba sp. a protozoan parasite, is a major public health problem in tropical and subtropical countries. the symptomatic patients are treated by specific chemotherapy. however, there are reports of treatment failure in some cases suggesting the possibility of drug resistance. the present study was therefore planned to assess the presence and expression of mrna of multidrug resistance (mdr) gene in clinical isolates of entamoeba histolytica and e. dispar. | 2006 | 17015933 |
| expression and function of a family of transmembrane kinases from the protozoan parasite entamoeba histolytica. | the signaling proteome of entamoeba histolytica is made of transmembrane kinases (tmks) that are rarely found in unicellular eukaryotes. there are 90 tmk genes reported for e. histolytica, and these have been grouped into nine distinct families based on motifs present on both extracellular and kinase domains. of these, the b1 family was chosen for further analysis. genomic sequencing revealed the presence of 28 members belonging to this family. genes corresponding to the majority of these were t ... | 2006 | 16926429 |
| toward a rational design of selective multi-trypanosomatid inhibitors: a computational docking study. | compound v7, a benzothiazole which was recently found as selective inhibitor of trypanosomal tims, was docked into tims from trypanosoma cruzi, trypanosoma brucei, entamoeba histolytica, plasmodium falciparum, yeast, and human. structural analyses revealed the importance of the accessibility to the two aromatic clusters located at the dimer's interface for the selective inhibition of trypanosomal tims. thus, it was found that different accessibilities of the protein interface of tims plays an im ... | 2006 | 16997551 |
| evaluation of entamoeba histolytica antigen and antibody point-of-care tests for the rapid diagnosis of amebiasis. | the bedside diagnosis of amebiasis could improve patient care. in bangladesh and vietnam, a novel and simple-to-use entamoeba histolytica rapid antigen test had 97% sensitivity and 100% specificity compared to the results of a standard enzyme-linked immunosorbent assay antigen detection method, and a rapid antibody test had 89 to 100% sensitivity and 89 to 95% specificity. | 2006 | 17035498 |
| preliminary evidence on existence of transplasma membrane electron transport in entamoeba histolytica trophozoites: a key mechanism for maintaining optimal redox balance. | entamoeba histolytica, an amitochondriate parasitic protist, was demonstrated to be capable of reducing the oxidized form of alpha-lipoic acid, a non permeable electron acceptor outside the plasma membrane. this transmembrane reduction of non permeable electron acceptors with redox potentials ranging from -290 mv to +360 mv takes place at neutral ph. the transmembrane reduction of non permeable electron acceptors was not inhibited by mitochondrial electron transport inhibitors such as antimycin ... | 2006 | 17039394 |
| identification of insertion hot spots for non-ltr retrotransposons: computational and biochemical application to entamoeba histolytica. | the genome of the human pathogen entamoeba histolytica contains non-long terminal repeat (ltr) retrotransposons, the ehlines and ehsines, which lack targeted insertion. we investigated the importance of local dna structure, and sequence preference of the element-encoded endonuclease (en) in selecting target sites for retrotransposon insertion. pre-insertion loci were tested computationally to detect unique features based on dna structure, thermodynamic considerations and protein interaction meas ... | 2006 | 17040894 |
| use of molecular tools to distinguish entamoeba histolytica and entamoeba dispar infection among the aborigines in cameron highlands. | amoebiasis is an infectious diseased caused by parasitic one-celled protozoan called entamoeba histolytica. numerous protozoa also can inhabit the gastro-intestinal tract of human. majority of these protozoa are non-pathogenic commensals or only causes disease under certain circumstances. morphologically, e. histolytica, the invasive form, share the same characteristic with the nonpathogenic form, e. dispar. both strains can be distinguished by using dna identification. many previous researches ... | 2006 | 17041549 |