Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| mycobacterium tuberculosis dna increases vitamin d receptor mrna expression and the production of nitric oxide and cathelicidin in human monocytes. | the innate immune response to tuberculosis infection may involve the increased production of nitric oxide and cathelicidin due to the up-regulated expression of the vitamin d receptor (vdr), though this proposed mechanism remains controversial. the aim of this study was to determine how the exposure of human monocytes to mycobacterium tuberculosis (m. tuberculosis) dna affects the production of nitric oxide and cathelicidin, as well as the expression of vdr. | 2017 | 26715892 |
| hemophagocytic lymphohistiocytosis: an unusual complication in disseminated mycobacterium tuberculosis. | hemophagocytic lymphohistiocytosis (hlh) is an uncommon, potentially fatal, hyperinflammatory syndrome that may rarely complicate the clinical course of disseminated mycobacterium tuberculosis (mtb). the clinical course of tuberculosis-associated hlh (tb-hlh) has been reported to be unpredictable. | 2017 | 26664166 |
| analysis of hla association among north indian hiv-positive individuals co-infected with mycobacterium tuberculosis. | genetic variation in hla genes influence the immune response and may thus contribute to differential development of tuberculosis (tb) in hiv-infected individuals. the study was designed to determine whether hla polymorphisms influence the development of mycobacterium tuberculosis infection in hiv-infected individuals. | 2017 | 26628757 |
| status of multidrug resistant tuberculosis (mdr-tb) among the sahariya tribe of north central india. | the incidence/prevalence of tuberculosis (tb) is reported to be high in the sahariya tribe of north central india. the outbreaks of different drug-resistant isolates of mycobacterium tuberculosis emphasized the need for continuous monitoring of resistance to anti-tuberculosis drugs. this study aimed to assess the profile of multidrug resistant tb among the sahariya tribe and their non-tribal neighbors for first line drugs through field-based investigations. | 2017 | 26775848 |
| improving t-cell assays for diagnosis of latent tb infection: confirmation of the potential role of testing interleukin-2 release in iranian patients. | since gamma interferon release assays (igras) cannot differentiate between active tuberculosis and latent tuberculosis infection (ltbi), development of rapid and specific diagnosis tools are essential for discriminating between active tuberculosis (tb) from ltbi. both igras are based on mycobacterium tuberculosis-specific antigens, namely, early secretory antigenic target 6 (esat-6) and 10kda culture filtrate (cfp-10). the aim of this study was to evaluate the potential value of il-2 secretion b ... | 2017 | 26786720 |
| [diagnostic strategies in the tuberculosis clinic of the hospital general la raza national medical center]. | in order to diagnose tb infection, tuberculin skin test and interferon gamma release assay are available. the tuberculin test has a sensitivity of 99 % and a specificity of 95 %. for the detection of interferon gamma in blood there are currently two tests available: tbgold quantiferon-in-tube (with a sensitivity of 0.70 and a specificity of 0.90), and t-spot-tb (sensitivity 0.90 and specificity 0.93). to diagnose the disease, a microscopy of direct smears for acid-fast bacilli is used if the phy ... | 2017 | 26820214 |
| [comment on an article: comparative genomic analysis of peruvian strains of mycobacterium tuberculosis]. | 2017 | 28327862 | |
| mycobacterium tuberculosis infection, immigration status, and diagnostic discordance: a comparison of tuberculin skin test and quantiferon-tb gold in-tube test among immigrants to the u.s. | we used a recent source of nationally representative population data on tuberculosis (tb) infection to characterize concordance between the tuberculin skin test (tst) and the quantiferon-tb gold in-tube (qft-git) blood test for immigrants in the united states. | 2017 | 26957665 |
| development of an effective delivery system for intranasal immunization against mycobacterium tuberculosis esat-6 antigen. | introduction the early secreted antigenic target 6-kda protein (esat-6) plays an important role in immune protection against tuberculosis. owing to its great potential to increase immune response, chitosan can be considered as a suitable biodegradable polymer for intranasal administration. methods the physiochemical properties of the nanoparticle were measured in vitro. two weeks after the last intranasal administration, blood samples were collected and specific igg, ifn-gama, and il-4 levels we ... | 2017 | 26924121 |
| in vitro-in vivo evaluation of novel co-spray dried rifampicin phospholipid lipospheres for oral delivery. | the objective of this study comprises of developing novel co-spray dried rifampicin phospholipid lipospheres (sdrpl) to investigate its influence on rifampicin solubility and oral bioavailability. solid-state techniques were employed to characterize the liposphere formulation. sdrpl solubility was determined in distilled water. bactec 460tb system was employed to evaluate sdrpl antimycobacterial activity. the oral bioavailability of the lipospheres was evaluated in sprague dawley rats. lipospher ... | 2017 | 26902373 |
| evaluating shared laboratory services: detecting mycobacterium tuberculosis complex and drug resistance using molecular and culture-based methods. | we explored sharing nucleic acid amplification testing (naat) for detection of mycobacterium tuberculosis complex (mtbc) and molecular and phenotypic drug susceptibility testing between two state public health tuberculosis (tb) laboratories, and evaluated turnaround times and cost-effectiveness. | 2017 | 26843677 |
| non-tuberculous mycobacteria: baseline data from three sites in papua new guinea, 2010-2012. | to determine the proportion of non-tuberculous mycobacteria (ntm) in samples of pulmonary tuberculosis (tb) cases from papua new guinea who were diagnosed using acid-fast microscopy. | 2017 | 26798558 |
| genetics-directed drug discovery for combating mycobacterium tuberculosis infection. | mycobacterium tuberculosis (mtb), the pathogen of tuberculosis (tb), is one of the most infectious bacteria in the world. the traditional strategy to combat tb involves targeting the pathogen directly; however, the rapid evolution of drug resistance lessens the efficiency of this anti-tb method. therefore, in recent years, some researchers have turned to an alternative anti-tb strategy, which hinders mtb infection through targeting host genes. in this work, using a theoretical genetic analysis, ... | 2017 | 26900080 |
| tuberculosis and cardiovascular disease: linking the epidemics. | the burden of tuberculosis and cardiovascular disease (cvd) is enormous worldwide. cvd rates are rapidly increasing in low- and middle-income countries. public health programs have been challenged with the overlapping tuberculosis and cvd epidemics. monocyte/macrophages, lymphocytes and cytokines involved in cellular mediated immune responses against mycobacterium tuberculosis are also main drivers of atherogenesis, suggesting a potential pathogenic role of tuberculosis in cvd via mechanisms tha ... | 2017 | 26835156 |
| everolimus-induced activation of latent mycobacterium tuberculosis infection in a patient with metastatic renal cell carcinoma. | 2017 | 26837008 | |
| an overview of phytotherapeutic approaches for the treatment of tuberculosis. | tuberculosis (tb) is highly infectious disease causing morbidity and death. its causative organism is a contagious bacterium, mycobacterium tuberculosis. the incidence of tuberculosis is increasing worldwide due to the emergence of drug resistance bacteria. the resistance is being developed in mycobacterium tuberculosis against both the first line as well as the second line drugs used for the treatment. the tuberculosis control programme is being complicated and failed to get the desired impact ... | 2017 | 27145855 |
| utility of mpt64 antigen test for differentiating mycobacteria: can correlation with liquid culture smear morphology add further value? | clinical presentation of mycobacterium tuberculosis complex (mtbc) and non-tuberculous mycobacteria (ntm) infections may or may not be the same, but the treatment is always different. hence accurate differentiation between mtbc and ntm is of utmost importance. | 2017 | 27166037 |
| bilateral pleural effusion in a hiv negative patient - tb or not tb? | pleural tuberculosis is an infrequent cause of respiratory illness in europe and usually presents unilaterally. we present the case of a young, immunocompetent sailor from the phillippines, who presented with bilateral pleural fluid caused by mycobacterium tuberculosis infection in the netherlands. in addition challenges in the diagnostic process are discussed. | 2017 | 27040935 |
| pet/ct imaging of mycobacterium tuberculosis infection. | tuberculosis has a high morbidity and mortality worldwide. mycobacterium tuberculosis (mtb) has a complex pathophysiology; it is an aerobic bacillus capable of surviving in anaerobic conditions in a latent state for a very long time before reactivation to active disease. in the latent tuberculosis infection, the individual has no clinical evidence of active disease, but exhibits a hypersensitive response to proteins of mtb. only some 5-10 % of latently infected individuals appear to have reactiv ... | 2017 | 27077068 |
| evaluation of resistance acquisition during tuberculosis treatment using whole genome sequencing. | tuberculosis (tb) is still considered a major global public health problem in the world and there is a concern about the worldwide increase of drug-resistance (dr). this paper describes the analysis of three mycobacterium tuberculosis isolates from a single patient collected over a long treatment period of time. dr was initially investigated through phenotypic testing, followed by line probe assays (lpas) and whole genome sequencing (wgs). it presents an intriguing situation where a multidrug-re ... | 2017 | 27004922 |
| lysine succinylation of mycobacterium tuberculosis isocitrate lyase (icl) fine-tunes the microbial resistance to antibiotics. | lysine succinylation (ksucc) is a newly identified protein posttranslational modification (ptm), which may play an important role in cellular physiology. however, the role of lysine succinylation in antibiotic resistance remains elusive. isocitrate lyase (icl) is crucial for broad-spectrum antibiotics tolerance in mycobacterium tuberculosis (mtb). we previously found that mtbicl (rv0467) has at least three succinylated lysine residues, namely k189, k322, and k334.to explore the effect of succiny ... | 2017 | 27023679 |
| safety profile of anakinra in the management of rheumatologic, metabolic and autoinflammatory disorders. | anakinra is a biologic response modifier that competitively antagonises the biologic effects of interleukin-1, the ancestor pleiotropic proinflammatory cytokine produced by numerous cell types, found in excess in the serum, synovial fluid and any involved tissues of patients with many inflammatory diseases. the magnitude of the risk of different infections, including mycobacterium tuberculosis (mtb) infection, associated with the large use of anakinra in many rheumatologic, metabolic or autoinfl ... | 2017 | 26940286 |
| [evaluation of stool microscopy and culture to assist the diagnosis of pulmonary tuberculosis in a tuberculosis endemic country]. | the aim of this study is to evaluate the performance of stool microscopy and culture in the diagnosis of pulmonary tuberculosis (ptb). | 2017 | 26948101 |
| utility of multiplex real-time pcr in the diagnosis of extrapulmonary tuberculosis. | the diagnosis of extrapulmonary tuberculosis is still a challenge because of its pauci-bacillary nature. the aim of the study was to evaluate the role of a multiplex pcr assay in the diagnosis of extrapulmonary tuberculosis and to compare the efficiency of two targets, is6110 and mpb64 to detect mycobacterium tuberculosis. | 2017 | 27020707 |
| innate cellular immunity in newly diagnosed pulmonary tuberculosis patients and during chemotherapy. | leukocyte migration (lm) and intracellular killing aspects of the innate immune response play important roles in protection against and containment and cure of mycobacterium tuberculosis infection, and thus may be exploited as immunotherapeutic targets to improve the management and treatment outcomes of patients with tuberculosis (tb). | 2017 | 27036724 |
| detection of mycobacterium tuberculosis complex by nested polymerase chain reaction in pulmonary and extrapulmonary specimens. | to compare the performance of nested polymerase chain reaction (npcr) with that of cultures in the detection of the mycobacterium tuberculosis complex in pulmonary and extrapulmonary specimens. | 2017 | 24473765 |
| nuclear imaging: a powerful novel approach for tuberculosis. | nearly 20 years after the world health organization declared tuberculosis (tb) a global public health emergency, tb still remains a major global threat with 8.6 million new cases and 1.3 million deaths annually. mycobacterium tuberculosis adapts to a quiescent physiological state, and is notable for complex interaction with the host, producing poorly-understood disease states ranging from latent infection to fully active disease. of the approximately 2.5 billion people latently infected with m. ... | 2017 | 25195017 |
| [latent tuberculosis infection: prevalence amongst healthcare students at the sapienza university of rome (italy)]. | healthcare workers, including medical, dentistry and nursing students, have an increased risk of mycobacterium tuberculosis infection. in this study the prevalence of tuberculin skin test (tst) positivity (defined as induration ≥ 5 mm) among healthcare students enrolled at the sapienza university of rome (italy) in the years 2011-2012 was evaluated. overall, 2,519 students with a mean age of 20.6 years were tested, of whom 62.4% were female, 0.4% were vaccinated with bcg and 4.6% were foreign-bo ... | 2017 | 25353269 |
| the cell envelope glycoconjugates of mycobacterium tuberculosis. | tuberculosis (tb) remains the second most common cause of death due to a single infectious agent. the cell envelope of mycobacterium tuberculosis (mtb), the causative agent of the disease in humans, is a source of unique glycoconjugates and the most distinctive feature of the biology of this organism. it is the basis of much of mtb pathogenesis and one of the major causes of its intrinsic resistance to chemotherapeutic agents. at the same time, the unique structures of mtb cell envelope glycocon ... | 2017 | 24915502 |
| mycobacterium tuberculosis resistance to antituberculosis drugs in mozambique. | to determine the drug resistance profile of mycobacterium tuberculosis in mozambique. | 2017 | 24831398 |
| cutaneous tuberculosis: epidemiologic, etiopathogenic and clinical aspects - part i. | cutaneous tuberculosis (ctb) is the result of a chronic infection by mycobacterium tuberculosis, m. ovis and occasionally by the calmette-guerin bacillus. the clinical manifestations are variable and depend on the interaction of several factors including the site of infection and the host's immunity. this article revises the current knowledge about this disease's physiopathology and immunology as well as detailing the possible clinical presentations. | 2017 | 24770496 |
| intra-abdominal mycobacterium tuberculosis infection in a dog. | 2017 | 24689658 | |
| isolation and evaluation of the enantiospecific antitubercular activity of a novel triazole compound. | cyclohex-3-enyl(5-phenyl-4h-1,2,4-triazol-3-yl)methanol (msdrt 12) is a novel triazole-based antitubercular compound with two chiral centers. to evaluate the enantiospecific antitubercular activity, the four stereoisomers were isolated using preparative chiral chromatography and the individual stereoisomers were evaluated using the resazurin microtiter assay method (rema) and a microbroth dilution technique against the mycobacterium tuberculosis h37rv strain. isomer iii of msdrt 12 was found to ... | 2017 | 24634844 |
| qsar and molecular docking studies of oxadiazole-ligated pyrrole derivatives as enoyl-acp (coa) reductase inhibitors. | a quantitative structure-activity relationship model was developed on a series of compounds containing oxadiazole-ligated pyrrole pharmacophore to identify key structural fragments required for anti-tubercular activity. two-dimensional (2d) and three-dimensional (3d) qsar studies were performed using multiple linear regression (mlr) analysis and k-nearest neighbour molecular field analysis (knn-mfa), respectively. the developed qsar models were found to be statistically significant with respect ... | 2017 | 24634843 |
| pathogen roid rage: cholesterol utilization by mycobacterium tuberculosis. | the ability of science and medicine to control the pathogen mycobacterium tuberculosis (mtb) requires an understanding of the complex host environment within which it resides. pathological and biological evidence overwhelmingly demonstrate how the mammalian steroid cholesterol is present throughout the course of infection. better understanding mtb requires a more complete understanding of how it utilizes molecules like cholesterol in this environment to sustain the infection of the host. cholest ... | 2017 | 24611808 |
| mycobacterium tuberculosis dosr regulon gene rv2004c encodes a novel antigen with pro-inflammatory functions and potential diagnostic application for detection of latent tuberculosis. | approximately 1.7 billion people in the world harbor latent mycobacterium tuberculosis (mtb) with a substantial risk of progression to clinical outcome. containment of these seed beds of mtb is essential to eliminate tuberculosis completely in high burden settings such as india. hence, there is an urgent need for the identification of new serological markers for detection or vaccine candidates to prevent latent tuberculosis infection (ltbi). dosr regulon antigens of mtb might serve as attractive ... | 2017 | 28694808 |
| medicinal plants used in the treatment of tuberculosis - ethnobotanical and ethnopharmacological approaches. | tuberculosis is a highly infectious disease declared a global health emergency by the world health organization, with approximately one third of the world's population being latently infected with mycobacterium tuberculosis. tuberculosis treatment consists in an intensive phase and a continuation phase. unfortunately, the appearance of multi drug-resistant tuberculosis, mainly due to low adherence to prescribed therapies or inefficient healthcare structures, requires at least 20months of treatme ... | 2017 | 28694178 |
| systematic review and meta-analysis of the nitrate reductase assay for drug susceptibility testing of mycobacterium tuberculosis and the detection limits in liquid medium. | recently, the need for rapid, reliable, and low-cost drug susceptibility testing (dst) methods has increased due to the emergence of multidrug-resistant mycobacterium tuberculosis. colorimetric methods of dst provide results more quickly than standard culture methods and are inexpensive than molecular methods. thus, colorimetric methods, such as the nitrate reductase assay (nra), are being recommended. we searched medline pubmed for reports on the nra for dst of m. tuberculosis written in englis ... | 2017 | 28694139 |
| hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins. | improved methods for detailed characterization of complex glycoproteins are required in the growing sector of biopharmaceuticals. hydrophilic interaction liquid chromatography (hilic) coupled to high resolution (hr) time-of-flight mass spectrometric (tof-ms) detection was examined for the characterization of intact neo-glycoproteins prepared by chemical conjugation of synthetic saccharides to the lysine residues of selected recombinant proteins. the separation performances of three different ami ... | 2017 | 28693734 |
| evaluation of the relationship between marco and cd36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a chinese han population. | gene polymorphisms impact greatly on a person's susceptibility to pulmonary tuberculosis (ptb). macrophage receptor with collagenous structure (marco) and cd36 are two scavenger receptors (srs) that can recognize mycobacterium tuberculosis (mtb) and play a key role in tuberculosis infection. gene polymorphisms of marco and cd36 may contribute to tuberculosis risk. | 2017 | 28693442 |
| whole genome sequencing of mycobacterium tuberculosis isolates from extrapulmonary sites. | tuberculosis (tb) remains one of the leading causes of morbidity and mortality worldwide. extrapulmonary tuberculosis (eptb) constitutes around 15-20% of tb cases in immunocompetent individuals. extrapulmonary sites that are affected by tb include bones, lymph nodes, meningitis, pleura, and genitourinary tract. whole genome sequencing has emerged as a powerful tool to map genetic diversity among mycobacterium tuberculosis (mtb) isolates and identify the genomic signatures associated with drug re ... | 2017 | 28692415 |
| structural and biophysical characterization of the mycobacterium tuberculosis protein rv0577, a protein associated with neutral red staining of virulent tuberculosis strains and homologue of the streptomyces coelicolor protein kbpa. | mycobacterium tuberculosis protein rv0577 is a prominent antigen in tuberculosis patients, the component responsible for neutral red staining of virulent strains of m. tuberculosis, a putative component in a methylglyoxal detoxification pathway, and an agonist of toll-like receptor 2. it also has an amino acid sequence that is 36% identical to that of streptomyces coelicolor afsk-binding protein a (kbpa), a component in the complex secondary metabolite pathways in the streptomyces genus. to gain ... | 2017 | 28692281 |
| the emergence of phenolic glycans as virulence factors in mycobacterium tuberculosis. | tuberculosis is the leading infectious cause of mortality worldwide. the global epidemic, caused by mycobacterium tuberculosis, has prompted renewed interest in the development of novel vaccines for disease prevention and control. the cell envelope of m. tuberculosis is decorated with an assortment of glycan structures, including glycolipids, that are involved in disease pathogenesis. phenolic glycolipids and the structurally related para-hydroxybenzoic acid derivatives display potent immunomodu ... | 2017 | 28692249 |
| case report of a lupus patient with a severe flare and miliary tuberculosis: need for proper guidelines for management. | systemic lupus erythematosus (sle) is an autoimmune disease that is frequently treated with high doses of corticosteroids and other immunosuppressive drugs. thus patients with sle are at increased risk for infections with several pathogens including mycobacterium tuberculosis. there are no established guidelines available for treatment of tuberculosis in sle patients with high disease activity due to lack of relevant studies and management based more on physician expertise. we report a case of a ... | 2017 | 28690859 |
| identification of inhibitors against α-isopropylmalate synthase of mycobacterium tuberculosis using docking-mm/pbsa hybrid approach. | α-isopropylmalate synthase (α-ipms) encoded by leua in mycobacterium tuberculosis (m.tb) is involved in the leucine biosynthesis pathway and is extremely critical for the synthesis of branched-chain amino acids (leucine, isoleucine and valine). α-ipms activity is required not only for the proliferation of m.tb but is also indispensable for its survival during the latent phase of infection. it is absent in humans and is widely regarded as one of the validated drug targets against tuberculosis (tb ... | 2017 | 28690380 |
| [diagnostic value of different detection methods for mycobacterium tuberculosis in bronchoalveolar lavage fluid from pulmonary tuberculosis patients]. | to investigate the diagnostic value of different detection methods for mycobacterium tuberculosis in bronchoalveolar lavage fluid (balf) from patients with pulmonary tuberculosis. methods: balf from100 patients in changsha central hospital from january 2013 to december 2015 was collected. among 100 patients, 65 cases were clinically diagnosed as tuberculosis, and 35 cases served as control. balf smear method, polymerase chain reaction (pcr) and membrane reverse dot blot (rdb) were used for sync ... | 2017 | 28690221 |
| combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis. | early diagnosis of tuberculosis is of major clinical importance. among 4733 clinical specimens collected from 3363 patients and subjected to ziehl-neelsen microscopy, 4109 were inoculated onto löwenstein-jensen slants and 3139 in bactec/9000mb. polymerase chain reaction (pcr) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 mycobacterium tuberculosis complex pcr-positive for isoniazid and rifampicin resistance detection specimens (genotype mtbdrplus). recove ... | 2017 | 28689813 |
| invasive mucinous adenocarcinoma with lepidic-predominant pattern coexisted with tuberculosis: a case report. | we observed a rare case of invasive mucinous adenocarcinoma (ima) with a lepidic-predominant pattern accompanied by pulmonary tuberculosis. an 85-year-old man with repeated cough and sputum was admitted to xinhua hospital. t-spot test result was 212 pg/ml (reference value of negative is < 14 pg/ml), mycobacterium tuberculosis culture was positive, and tuberculin skin test (ppd) was negative (skin induration < 5 mm). the patient was treated with several courses of antibiotics and anti-tuberculosi ... | 2017 | 28687977 |
| a review of eleven cases of tuberculosis presenting as sternal wound abscess after open heart surgery. | sternal wound infection with mycobacterium tuberculosis is an uncommon yet highly challenging disease that can be quite insidious with various presentations. we hereby provide a review of 10 cases in current literature and describe an additional case which illustrates the difficulties associated with diagnosis. | 2017 | 28687049 |
| proteomic profile of mycobacterium tuberculosis after eupomatenoid-5 induction reveals potential drug targets. | we investigated a proteome profile, protein-protein interaction and morphological changes of mycobacterium tuberculosis after different times of eupomatenoid-5 (eup-5) induction to evaluate the cellular response to the drug-induced damages. | 2017 | 28686056 |
| human genetic factors in tuberculosis: an update. | tuberculosis (tb) is a major threat to human health, especially in many developing countries. human genetic variability has been recognised to be of great relevance in host responses to mycobacterium tuberculosis infection and in regulating both the establishment and the progression of the disease. an increasing number of candidate gene and genome-wide association studies (gwas) have focused on human genetic factors contributing to susceptibility or resistance to tb. to update previous reviews o ... | 2017 | 28685916 |
| conjugated and entrapped hpma-pla nano-polymeric micelles based dual delivery of first line anti tb drugs: improved and safe drug delivery against sensitive and resistant mycobacterium tuberculosis. | first line antitb drugs have several physical and toxic manifestations which limit their applications. rif is a hydrophobic drug and has low water solubility and inh is hepatotoxic. the main objective of the study was to synthesize, characterize hpma-pla co-polymeric micelles for the effective dual delivery of inh and rif. | 2017 | 28685299 |
| evaluation of mycotube, a modified version of lowenstein-jensen (lj) medium, for efficient recovery of mycobacterium tuberculosis (mtb). | timely diagnosis of tuberculosis (tb), caused by mycobacterium tuberculosis (mtb), is only achieved for ~58% cases. an improved, accurate, time- and cost-effective method for bacteriological confirmation of mtb is necessary. we evaluated mycotube, a new variant of lowenstein-jensen (lj) culture medium, by comparing it with mycobacterium growth indicator tube (mgit) 960 (gold standard), local lj, and biomérieux lj-t in terms of isolation rate and time-to-growth. pulmonary and extra-pulmonary samp ... | 2017 | 28685188 |
| erratum: data intensive genome level analysis for identifying novel, non-toxic drug targets for multi drug resistant mycobacterium tuberculosis. | this corrects the article doi: 10.1038/srep46595. | 2017 | 28684876 |
| complete genome sequences of three representative mycobacterium tuberculosis beijing family strains belonging to distinct genotype clusters in hanoi, vietnam, during 2007 to 2009. | we present here three complete genome sequences of mycobacterium tuberculosis beijing family strains isolated in hanoi, vietnam. these three strains were selected from major genotypic clusters (15-miru-vntr) identified in a previous population-based study. we emphasize their importance and potential as reference strains in this asian region. | 2017 | 28684565 |
| longitudinal profiling reveals a persistent intestinal dysbiosis triggered by conventional anti-tuberculosis therapy. | effective treatment of mycobacterium tuberculosis (mtb) infection requires at least 6 months of daily therapy with multiple orally administered antibiotics. although this drug regimen is administered annually to millions worldwide, the impact of such intensive antimicrobial treatment on the host microbiome has never been formally investigated. here, we characterized the longitudinal outcome of conventional isoniazid-rifampin-pyrazinamide (hrz) tb drug administration on the diversity and composit ... | 2017 | 28683818 |
| host-directed therapeutics as novel angle for tuberculosis treatment. | despite significant efforts to improve tuberculosis (tb) treatment, it remains a worldwide infectious disease due to the limitations of current tb regimens. recent work on novel tb treatment strategies has suggested that directly targeting host factors is beneficial for tb treatment. such strategies, termed host-directed therapeutics (hdts), focus on host-pathogen interactions. hdts are more beneficial than existed tb drugs, which are limited the long durations of treatment and the emergence of ... | 2017 | 28683527 |
| proteomics of culture filtrate of prevalent mycobacterium tuberculosis strains: 2d-page map and maldi-tof/ms analysis. | although diverse efforts have been done to identify biomarkers for control of tuberculosis using laboratory strain mycobacterium tuberculosis h37rv, the disease still poses a threat to mankind. there are many emerging m. tuberculosis strains, and proteomic profiling of these strains might be important to find out potential targets for diagnosis and/or prevention of tuberculosis. we evaluated the comparative proteomic profiling of culture filtrate (cf) proteins from prevalent m. tuberculosis stra ... | 2017 | 28683213 |
| synthesis and immunological studies of the lipomannan backbone glycans found on the surface of mycobacterium tuberculosis. | investigations into novel bacterial drug targets and vaccines are necessary to overcome tuberculosis. lipomannan (lm), found on the surface of mycobacterium tuberculosis (mtb), is actively involved in the pathogenesis and survival of mtb. here, we report for the first time a rapid synthesis and biological activities of an lm glycan backbone, α(1-6)mannans. the rapid synthesis is achieved via a regio- and stereoselective ring opening polymerization to generate multiple glycosidic bonds in one sim ... | 2017 | 28682637 |
| synthesis of a 3-amino-2,3-dihydropyrid-4-one and related heterocyclic analogues as mechanism-based inhibitors of bioa, a pyridoxal phosphate-dependent enzyme. | amiclenomycin (acm) is a chemically unstable antibiotic with selective activity against mycobacterium tuberculosis (mtb) due to mechanism-based inhibition of bioa, a pyridoxal 5'-phosphate (plp)-dependent aminotransferase. the first-generation acm analogue dihydro-2-pyridone 1 maintains a similar bioactivation mechanism concluding with covalent labeling of the plp cofactor. to improve on 1, we report the synthesis of dihydro-4-pyranone 2, dihydro-4-pyridone 3, and dihydro-4-thiopyranone 13, whic ... | 2017 | 28682613 |
| development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of mycobacterium tuberculosis. | loop-mediated isothermal amplification (lamp) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. we present a novel strategy for the detection of lamp amplicons derived from mycobacterium tuberculosis by the use of au-nanoprobes. when applied to a total of 93 clinical specimens, the lamp assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. the au-nanoprobe augmented lamp test platform with its advantages o ... | 2017 | 28681828 |
| levels of circulating immune complexes containing mycobacterium tuberculosis-specific antigens in pulmonary tuberculosis and sarcoidosis patients. | the present study was conducted to understand the aetiological link between tuberculosis (tb) and sarcoidosis. sera from smear-positive tb subjects (n = 24), smear-negative tb subjects (n = 24), sarcoidosis patients (n = 24) and healthy controls (n = 24) were collected and circulating immune complexes were isolated. sandwich elisa was performed for detecting four highly specific mycobacterial regions of difference (rd) proteins (early secretory antigenic target 6 [esat6], 10 kda culture filtrate ... | 2017 | 28681824 |
| genotypic characterisation of mycobacterium tuberculosis isolates from tuberculous meningitis patients at a tertiary neurocare centre in southern india. | specific genotypes of mycobacterium tuberculosis (mtb) have been reported to cause outbreaks of pulmonary tuberculosis (tb) in geographical areas that are endemic to tb. however, since there is little epidemiological evidence on the association of particular genotypes that cause tuberculous meningitis (tbm), we sought to investigate the association of specific mtb strains with infection of the central nervous system (cns). | 2017 | 28681808 |
| disruption of key nadh-binding pocket residues of the mycobacterium tuberculosis inha affects dd-coa binding ability. | tuberculosis (tb) is a global health problem that affects over 10 million people. there is an urgent need to develop novel antimicrobial therapies to combat tb. to achieve this, a thorough understanding of key validated drug targets is required. the enoyl reductase inha, responsible for synthesis of essential mycolic acids in the mycobacterial cell wall, is the target for the frontline anti-tb drug isoniazid. to better understand the activity of this protein a series of mutants, targeted to the ... | 2017 | 28680153 |
| virtual screening, pharmacophore development and structure based similarity search to identify inhibitors against ider, a transcription factor of mycobacterium tuberculosis. | ider, an essential gene of mycobacterium tuberculosis, is an attractive drug target as its conditional knockout displayed attenuated growth phenotype in vitro and in vivo. to the best of our knowledge, no inhibitors of ider are identified. we carried out virtual screening of nci database against the ider dna binding domain followed by inhibition studies using emsa. nine compounds exhibited potent inhibition with nsc 281033 (i-20) and nsc 12453 (i-42) exhibiting ic50 values of 2 µg/ml and 1 µg/ml ... | 2017 | 28680150 |
| mechanism-based inactivator of isocitrate lyases 1 and 2 from mycobacterium tuberculosis. | isocitrate lyase (icl, types 1 and 2) is the first enzyme of the glyoxylate shunt, an essential pathway for mycobacterium tuberculosis (mtb) during the persistent phase of human tb infection. here, we report 2-vinyl-d-isocitrate (2-vic) as a mechanism-based inactivator of mtb icl1 and icl2. the enzyme-catalyzed retro-aldol cleavage of 2-vic unmasks a michael substrate, 2-vinylglyoxylate, which then forms a slowly reversible, covalent adduct with the thiolate form of active-site cys191 2-vic disp ... | 2017 | 28679637 |
| orbital tuberculosis: clinicopathological correlation and diagnosis using pcr in formalin-fixed tissues. | this article describes the clinicopathological correlation and challenges encountered in the diagnosis of orbital tuberculosis (tb). retrospective, interventional case series. a chart review of six patients who underwent biopsy for an orbital mass between january 2012 and december 2013 was performed. institutional review board approval was obtained for the study. clinical, radiological, and laboratory findings were documented, and response to antitubercular therapy (att) was noted. age at presen ... | 2017 | 28678592 |
| hla-e presents glycopeptides from the mycobacterium tuberculosis protein mpt32 to human cd8(+) t cells. | infection with mycobacterium tuberculosis (mtb), the bacterium that causes tuberculosis, remains a global health concern. both classically and non-classically restricted cytotoxic cd8(+) t cells are important to the control of mtb infection. we and others have demonstrated that the non-classical mhc i molecule hla-e can present pathogen-derived peptides to cd8(+) t cells. in this manuscript, we identified the antigen recognized by an hla-e-restricted cd8(+) t cell clone isolated from an mtb late ... | 2017 | 28676677 |
| the sigma factor sigd of mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments. | this work examined the expression of the septum site determining gene (ssd) of mycobacterium tuberculosis cdc1551 and its δsigd mutant under different growing conditions. the results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for sigd in the control of ssd expression mainly under lack-of-nutrients environments. furthermore, we elucidated a putative link between ssd expression and cell elongation o ... | 2017 | 28675242 |
| genexpert technology. a breakthrough for the diagnosis of tuberculous pericarditis and pleuritis in less than 2 hours. | to evaluate the diagnostic validity of genexpert for the detection of mycobacterium tuberculosis (mtb) in pericardial and pleural effusions samples. | 2017 | 28674714 |
| mycobacterium tuberculosis endocarditis in native valves. | 2017 | 28674587 | |
| comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in china. | to evaluate the diagnostic utilities of different methods for mycobacterium tuberculosis (m. tuberculosis) detection in pleural fluid that represent potentially cost-effective measures for resource-limited settings in china. we compared diagnostic characteristics of the ampsure simultaneous amplification and testing method, the bactec mgit 960 system, and acid-fast bacilli staining of specimen smears for detection of m. tuberculosis in pleural fluids of 438 patients with suspected tuberculous pl ... | 2017 | 28674427 |
| a novel 6-benzyl ether benzoxaborole is active against mycobacterium tuberculosis in vitro. | we identified a novel 6-benzyl ether benzoxaborole with potent activity against mycobacterium tuberculosis the compound had an mic of 2 μm in liquid medium. the compound was also able to prevent growth on solid medium at 0.8 μm and was active against intracellular bacteria (50% inhibitory concentration [ic50] = 3.6 μm) without cytotoxicity against eukaryotic cells (ic50 > 100 μm). we isolated resistant mutants (mic ≥ 100 μm), which had mutations in rv1683, rv3068c, and rv0047c. | 2017 | 28674058 |
| rare presentation of intractable tuberculous panophthalmitis with intraocular and intraorbital abscesses: a case report. | we report a rare presentation of extrapulmonary tuberculosis. | 2017 | 28673337 |
| crystallographic and solution structure of the n-terminal domain of the rel protein from mycobacterium tuberculosis. | modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppgpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. in mycobacterium tuberculosis (mtb), (p)ppgpp synthesis and degradation are carried out by the bifunctional enzyme mtrel, which consists of 738 residues, including an n-terminal hydrolase- and synthetase-domain (n-terminal domain or ntd) and a c-terminus with a ri ... | 2017 | 28672070 |
| epidemiology of musculoskeletal tuberculosis in an area with high disease prevalence. | retrospective observational study. | 2017 | 28670408 |
| an unusual triad of hemophagocytic syndrome, lymphoma and tuberculosis in a non-hiv patient. | background lymphoma complicated with hemophagocytic syndrome and tuberculosis has been rarely reported. the clinical and radiological presentation of these potentially fatal conditions can be easily confused and there is a potential for misdiagnosis. case report we present a 58-year-old hispanic female who was admitted to the hospital with dizziness and fever. her initial admission diagnosis was severe sepsis secondary to community acquired pneumonia. she was started on intravenous antibiotics. ... | 2017 | 28669977 |
| investigation of pre-xdr beijing mycobacterium tuberculosis transmission to a healthcare worker in france, 2016. | a case of occupational contamination of a healthcare worker by a pre-extensively drug-resistant (pre-xdr) beijing strain of mycobacterium tuberculosis at the university hospital of montpellier, france is reported. the index case was identified using genetic fingerprinting of isolates. this report underscores the risk of healthcare-associated contamination by pre-xdr tuberculosis (tb) in low-incidence countries and the importance of molecular tools for tb care. it also calls for increased vigilan ... | 2017 | 28669673 |
| development of a novel lead that targets m. tuberculosis polyketide synthase 13. | widespread resistance to first-line tb drugs is a major problem that will likely only be resolved through the development of new drugs with novel mechanisms of action. we have used structure-guided methods to develop a lead molecule that targets the thioesterase activity of polyketide synthase pks13, an essential enzyme that forms mycolic acids, required for the cell wall of mycobacterium tuberculosis. our lead, tam16, is a benzofuran class inhibitor of pks13 with highly potent in vitro bacteric ... | 2017 | 28669536 |
| high incidence of fluoroquinolone resistance and effect of efflux pump inhibitors on moxifloxacin resistance among mycobacterium tuberculosis isolates causing urinary tract infection in taiwan. | this study explored the prevalence of urinary tract tuberculosis (utb) and whether efflux pump activation accounts for resistance to moxifloxacin in taiwan. of 3034 patients with culture-confirmed tb from 2005-2012, 47 patients (1.5%) with utb were included in this study. minimum inhibitory concentrations (mics) of moxifloxacin were determined in the presence and absence of efflux pump inhibitors (epis), including verapamil, reserpine and carbonyl cyanide 3-chlorophenylhydrazone (cccp). epi resp ... | 2017 | 28668684 |
| cross-linking of dicyclotyrosine by the cytochrome p450 enzyme cyp121 from mycobacterium tuberculosis proceeds through a catalytic shunt pathway. | cyp121, the cytochrome p450 enzyme in mycobacterium tuberculosis that catalyzes a single intramolecular c-c cross-linking reaction in the biosynthesis of mycocyclosin, is crucial for the viability of this pathogen. this c-c coupling reaction represents an expansion of the activities carried out by p450 enzymes distinct from oxygen insertion. although the traditional mechanism for p450 enzymes has been well studied, it is unclear whether cyp121 follows the general p450 mechanism or uses a differe ... | 2017 | 28667013 |
| genomic epidemiology of a major mycobacterium tuberculosis outbreak: retrospective cohort study in a low-incidence setting using sparse time-series sampling. | since 1992, denmark has documented the largest outbreak of tuberculosis in scandinavia ascribed to a single genotype, termed c2/1112-15. as of spring 2017, the international reference laboratory of mycobacteriology in copenhagen has collected and identified isolates from more than a thousand cases belonging to this outbreak via routine mycobacterial interspersed repetitive units-variable number of tandem repeats typing. here, we present a retrospective analysis of the c2/1112-15 dataset, based o ... | 2017 | 28666374 |
| quaternary structure is an essential component that contributes to the sophisticated allosteric regulation mechanism in a key enzyme from mycobacterium tuberculosis. | the first enzyme of the shikimate pathway, 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (dah7ps), adopts a range of distinct allosteric regulation mechanisms in different organisms, related to different quaternary assemblies. dah7ps from mycobacterium tuberculosis (mtudah7ps) is a homotetramer, with the allosteric sites in close proximity to the interfaces. here we examine the importance of the quaternary structure on catalysis and regulation, by amino acid substitution targeting the tet ... | 2017 | 28665948 |
| polymerase chain reaction and its correlation with clinical features and treatment response in tubercular uveitis. | correlation of results of polymerase chain reaction for mycobacterium tuberculosis (mtb pcr) with clinical features and treatment response in tubercular uveitis. | 2017 | 28665743 |
| biochemical investigation of rv3404c from mycobacterium tuberculosis. | the causative agent of tuberculosis, mycobacterium tuberculosis, is a bacterium with a complex cell wall and a complicated life cycle. the genome of m. tuberculosis contains well over 4000 genes thought to encode proteins. one of these codes for a putative enzyme referred to as rv3404c, which has attracted research attention as a potential virulence factor for over 12 years. here we demonstrate that rv3404c functions as a sugar n-formyltransferase that converts dtdp-4-amino-4,6-dideoxyglucose in ... | 2017 | 28665588 |
| cd1b-mycolic acid tetramers demonstrate t-cell fine specificity for mycobacterial lipid tails. | mycobacterium tuberculosis synthesizes a thick cell wall comprised of mycolic acids (ma), which are foreign antigens for human t cells. t-cell clones from multiple donors were used to determine the fine specificity of ma recognition by human αβ t cells. most cd1-presented lipid antigens contain large hydrophilic head groups comprised of carbohydrates or peptides that dominate patterns of t-cell specificity. ma diverges from the consensus antigen motif in that it lacks a head group. using multipl ... | 2017 | 28665555 |
| plant-derived antimicrobials to fight against multi-drug-resistant human pathogens. | antibiotic resistance is becoming a pivotal concern for public health that has accelerated the search for new antimicrobial molecules from nature. numbers of human pathogens have inevitably evolved to become resistant to various currently available drugs causing considerable mortality and morbidity worldwide. it is apparent that novel antibiotics are urgently warranted to combat these life-threatening pathogens. in recent years, there have been an increasing number of studies to discover new bio ... | 2017 | 28660459 |
| incidental unilateral tuberculous sacroiliitis detected by (18)f-fdg pet/ct in a patient with abdominal tuberculosis. | tuberculosis is a systemic disease which involves skeletal and articular system very rarely. osteoarticular tuberculosis commonly occurs in the vertebral column and more rarely in the sacroiliac joints. in this study, we report a 44-years-old male patient with low-grade fever, malabsorption syndrome, abdominal and pelvic ascites and low-back pain, that underwent (18)f-fdg pet/ct for identifying the cause of signs and symptoms after a negative abdominal ct and negative thorax radiography. the stu ... | 2017 | 28660225 |
| rna profiling analysis of the serum exosomes derived from patients with active and latent mycobacterium tuberculosis infection. | tuberculosis (tb) has exceeded hiv as the most lethal infectious disease globally for two consecutive years. moreover, one third of the world's population is estimated to have latent tuberculosis infection (ltbi). this is mainly because of difficulties associated with diagnosis and treatment for both tb and ltbi patients. exosomes provide a promising research tool for tb diagnosis and treatment because they are released from various cells containing valuable biochemical information related to di ... | 2017 | 28659881 |
| bioa mutant of mycobacterium tuberculosis shows severe growth defect and imparts protection against tuberculosis in guinea pigs. | owing to the devastation caused by tuberculosis along with the unsatisfactory performance of the bacillus calmette-guérin (bcg) vaccine, a more efficient vaccine than bcg is required for the global control of tuberculosis. a number of studies have demonstrated an essential role of biotin biosynthesis in the growth and survival of several microorganisms, including mycobacteria, through deletion of the genes involved in de novo biotin biosynthesis. in this study, we demonstrate that a bioa mutant ... | 2017 | 28658275 |
| molecular modeling studies and anti-tb activity of trisubstituted indolizine analogues; molecular docking and dynamic inputs. | a series of trisubstituted indolizine analogues has been designed as a result of a fragment-based approach to target the inhibition of mycobacterial enoyl-acyl carrier protein reductase. anti-tuberculosis (tb) screening of the characterized compounds by a resazurin microplate assay method revealed that ethyl group at second position of indolizine nucleus exhibited activity against susceptible and multidrug-resistant strains of mycobacterium tuberculosis at concentration of 5.5 and 11.3 μg/ml, re ... | 2017 | 28657441 |
| determination of redox sensitivity in structurally similar biological redox sensors. | redox stimuli govern a variety of biological processes. the relative sensitivity of redox sensors plays an important role in providing a calibrated response to environmental stimuli and cellular homeostasis. this cellular machinery plays a crucial role in the human pathogen mycobacterium tuberculosis as it encounters diverse microenvironments in the host. the redox sensory mechanism in m. tuberculosis is governed by two component and one-component systems, alongside a class of transcription fact ... | 2017 | 28657321 |
| assessing pharmacodynamic interactions in mice using the multistate tuberculosis pharmacometric and general pharmacodynamic interaction models. | the aim of this study was to investigate pharmacodynamic interactions in mice infected with mycobacterium tuberculosis using population pharmacokinetics, the multistate tuberculosis pharmacometric (mtp) model, and the general pharmacodynamic interaction (gpdi) model. rifampicin, isoniazid, ethambutol or pyrazinamide were administered in monotherapy for 4 weeks. rifampicin and isoniazid showed effects in monotherapy, whereas the animals became moribund after 7 days with ethambutol or pyrazinamide ... | 2017 | 28657202 |
| role of esat-6 in renal injury by regulating microrna-155 expression via tlr4/myd88 signaling pathway in mice with mycobacterium tuberculosis infection. | the study aims to investigate the underlying mechanism involved in the early secretory antigenic target-6 (esat-6) in renal injury through regulation of the expression of mir-155 through the oll-like receptor (tlr)-4 (tlr4)/myeloid differentiation factor 88 (myd88) signaling pathway in mycobacterium tuberculosis (mtb)-infected mice. sixty c57bl/6 mice with mtb-induced renal injury were randomly assigned into control, mtb, mimic, inhibitor, inhibitor + esat6, and inhibitor + esat6 + tak242 groups ... | 2017 | 28655852 |
| identification of host proteins predictive of early stage mycobacterium tuberculosis infection. | the objective of this study was to identify blood-based protein biomarkers of early stage mycobacterium tuberculosis (mtb) infection. we utilized plasma and serum specimens from tb patients and their contacts (age≥12) enrolled in a household contact study in uganda. in the discovery phase cross-sectional samples from 104 hiv-uninfected persons classified as either active tb, latent mtb infection (ltbi), tuberculin skin test (tst) converters, or persistent tst-negative were analyzed. two hundred ... | 2017 | 28655597 |
| preparation and evaluation of potent pentafluorosulfanyl-substituted anti-tuberculosis compounds. | the global fight to stop tuberculosis (tb) remains a great challenge, particularly with the increase in drug-resistant strains and a lack of funding to support the development of new treatments. to bolster a precarious drug pipeline, we prepared a focused panel of eight pentafluorosulfanyl (sf5 ) compounds which were screened for their activity against mycobacterium tuberculosis (mtb) h37rv in three different assay conditions and media. all eight compounds had sub-micromolar potency, and four di ... | 2017 | 28654200 |
| rifampicin-resistant mycobacterium tuberculosis among tuberculosis-presumptive cases at university of gondar hospital, northwest ethiopia. | various studies have reported that the emergence of drug-resistant tuberculosis poses a significant threat to tuberculosis-control programs worldwide. rifampicin resistance is a surrogate marker of multidrug-resistant tuberculosis, since it reveals the presence of greater than 90% isoniazid resistance. evidence on rifampicin-resistant mycobacterium tuberculosis is scarce in the literature. | 2017 | 28652786 |
| exploiting the synthetic lethality between terminal respiratory oxidases to kill mycobacterium tuberculosis and clear host infection. | the recent discovery of small molecules targeting the cytochrome bc1 :aa3 in mycobacterium tuberculosis triggered interest in the terminal respiratory oxidases for antituberculosis drug development. the mycobacterial cytochrome bc1 :aa3 consists of a menaquinone:cytochrome c reductase (bc1 ) and a cytochrome aa3 -type oxidase. the clinical-stage drug candidate q203 interferes with the function of the subunit b of the menaquinone:cytochrome c reductase. despite the affinity of q203 for the bc1 :a ... | 2017 | 28652330 |
| il-6 and il-18 cytokine gene variants of pulmonary tuberculosis patients with co-morbid diabetes mellitus and their household contacts in hyderabad. | association of cytokine genes reflects their susceptibility towards infection and disease in household contacts (hhc) of pulmonary tuberculosis (ptb) patients. hyperglycemia, a common factor in diabetics might influence their risk towards mycobacterium tuberculosis infection and disease development. this study determines the association of il-6 and il-18 cytokine gene variants of tb patients with diabetes mellitus (tbdm) and their hhc in hyderabad. | 2017 | 28652186 |
| [identification and evaluation of t cell epitopes of rv0585c from mycobacterium tuberculosis]. | objective: to investigate the human t cell epitopes of mycobacterium (m.) tuberculosis rv0585c protein antigen and their immunogenicity and provide evidence for the development of specific tuberculosis immune diagnostic techniques and tuberculosis vaccine. methods: we synthesized peptides from m. tuberculosis rv0585c protein antigen predicted by te-predict and iedb human t cell epitope prediction tool. the cellular immunoreactivity of the predicted peptides was evaluated through elispot assay wi ... | 2017 | 28651408 |