Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| structure and variation of crispr and crispr-flanking regions in deleted-direct repeat region mycobacterium tuberculosis complex strains. | crispr and crispr-flanking genomic regions are important for molecular epidemiology of mycobacterium tuberculosis complex (mtbc) strains, and potentially for adaptive immunity to phage and plasmid dna, and endogenous roles in the bacterium. genotyping in the israel national mycobacterium reference center tel-aviv of over 1500 mtbc strains from 2008-2013 showed three strains with validated negative 43-spacer spoligotypes, that is, with putatively deleted direct repeat regions (deleted-dr/crispr r ... | 2017 | 28201993 |
| ca(2+)-dependent focal exocytosis of golgi-derived vesicles helps phagocytic uptake in macrophages. | the role of golgi apparatus during phagocytic uptake by macrophages has been ruled out in the past. notably, all such reports were limited to fcγ receptor-mediated phagocytosis. here, we unravel a highly devolved mechanism for recruitment of golgi-derived secretory vesicles during phagosome biogenesis, which was important for uptake of most cargos, except the igg-coated ones. we report recruitment of mannosidase-ii-positive golgi-derived vesicles during uptake of diverse targets, including latex ... | 2017 | 28174296 |
| characterization and function of mycobacterium tuberculosis h37rv lipase rv1076 (lipu). | lipids and lipases/esterases are essential for mycobacterium tuberculosis (mtb) survival and persistence, even virulence. mycobacterium tuberculosis h37rv rv1076 (lipu), a member of lipase family, is homologous to the human hormone sensitive lipase (hsl) based on the presence of conserved motif 'gxsxg'. to define the enzymatic characteristics of rv1076, the gene was cloned, and expressed in escherichia coli. the protein was purified for enzymatic characterization. lipu showed high specific activ ... | 2017 | 28164792 |
| rv0774c, an iron stress inducible, extracellular esterase is involved in immune-suppression associated with altered cytokine and tlr2 expression. | tuberculosis, one of the leading cause of death from infectious diseases, is caused by mycobacterium tuberculosis. the genome of m. tuberculosis has been sequenced and nearly 40% of the whole genome sequence was categorized as hypothetical. rv0774c was annotated as membrane exported hypothetical protein in tb database. in silico analysis revealed that rv0774c is a paralog of pe-pgrs multi gene family with 100 aa n-terminal domain similar to pe domain of pe-pgrs proteins. its c-terminal domain is ... | 2017 | 28161108 |
| role of reactive oxygen species (ros) in therapeutics and drug resistance in cancer and bacteria. | evading persistent drug resistance in cancer and bacteria is quintessential to restore health in humans, and impels intervention strategies. a distinct property of the cancer phenotype is enhanced glucose metabolism and oxidative stress. reactive oxygen species (ros) are metabolic byproducts of aerobic respiration and are responsible for maintaining redox homeostasis in cells. redox balance and oxidative stress are orchestrated by antioxidant enzymes, reduced thiols and nadp(h) cofactors, which ... | 2017 | 28135088 |
| rhodococcus erythropolis as a host for expression, secretion and glycosylation of mycobacterium tuberculosis proteins. | glycosylation is one of the most abundant posttranslational polypeptide chain modification in nature. although carbohydrate modification of protein antigens from many microbial pathogens constitutes important components of b cell epitopes, the role in t cell immunity is not completely understood. there is growing evidence about the importance of these modifications in host bacteria interactions in tuberculosis. it is known, that the sugars present in some mycobacterium tuberculosis glycoproteins ... | 2017 | 28103877 |
| engineering mycobacteria for the production of self-assembling biopolyesters displaying mycobacterial antigens for use as a tuberculosis vaccine. | tuberculosis (tb) is a disease caused by mycobacterium tuberculosis or mycobacterium bovis and still remains one of the world's biggest global health burdens. recently, engineered polyhydroxyalkanoate (pha) biobeads that were produced in both escherichia coli and lactococcus lactis and displayed mycobacterial antigens were found to induce significant cell-mediated immune responses in mice. we observed that such pha beads contained host cell proteins as impurities, which we hypothesized to have t ... | 2017 | 28087528 |
| anti-folates potentiate bactericidal effects of other antimicrobial agents. | synergies between sulfonamides and other antimicrobial agents have long been reported, but the reason still remains unclear. previously, vilchèze et al. found that, sulfamethoxazole (smx) could potentiate the bacterialcidal activity of isoniazid (inh) and rifampin (rif) in mycobacterium tuberculosis. to test if this was also the case in other bacteria, the ability to potentiate bactericidal effect of rif by smx was evaluated in escherichia coli, staphylococcus aureus, salmonella typhimurium and ... | 2017 | 28074051 |
| comparing galactan biosynthesis in mycobacterium tuberculosis and corynebacterium diphtheriae. | the suborder corynebacterineae encompasses species like corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as corynebacterium diphtheriae and mycobacterium tuberculosis, which cause devastating human diseases. a distinctive component of the corynebacterineae cell envelope is the mycolyl-arabinogalactan (mag) complex. the mag is composed of lipid mycolic acids, and arabinofuranose (araf) and galactofuranose (galf) carbohydrate residues. elucidat ... | 2017 | 28039359 |
| infections of the central nervous system after unrelated donor umbilical cord blood transplantation or human leukocyte antigen-matched sibling transplantation. | we analyzed the incidence, clinical characteristics, prognostic factors, and outcome of central nervous system (cns) infections in consecutive patients with receiving umbilical cord blood transplantation (ucbt) (n = 343) or hla-matched sibling donor stem cell transplantation (mst) (n = 366). thirty-four cns infections were documented at a median time of 116 days after transplantation (range, 7 to 1161). the cumulative incidence (ci) risk of developing a cns infection was .6% at day +30, 2.3% at ... | 2017 | 27794456 |
| strong anti-epstein barr virus (ebv) or cytomegalovirus (cmv) cellular immune responses predict survival and a favourable response to anti-tuberculosis therapy. | intact immune responses to cytomegalovirus (cmv) and epstein-barr virus (ebv) represent a biologically and clinically relevant correlate of 'immunological fitness' in humans. however, there is a lack of knowledge concerning anti-ebv or anti-cmv responses in patients with pulmonary tuberculosis (tb), in whom aberrant immune responses may promote progression of clinical disease. | 2017 | 28193504 |
| the antibiotic resistance crisis, with a focus on the united states. | beginning with the discovery of penicillin by alexander fleming in the late 1920s, antibiotics have revolutionized the field of medicine. they have saved millions of lives each year, alleviated pain and suffering, and have even been used prophylactically for the prevention of infectious diseases. however, we have now reached a crisis where many antibiotics are no longer effective against even the simplest infections. such infections often result in an increased number of hospitalizations, more t ... | 2017 | 28246379 |
| "infectious supercarelessness" in discussing antibiotic-resistant bacteria. | many bacterial pathogens are exhibiting resistance to increasing numbers of antibiotics making it much more challenging to treat the infections caused by these microbes. in many reports in the media and perhaps even in discussions among physicians and biomedical scientists, these bacteria are frequently referred to as "bugs" with the prefix "super" appended. this terminology has a high potential to elicit unjustified inferences and fails to highlight the broader evolutionary context. understandi ... | 2017 | 28174759 |
| establishment of multiplex solid-phase strip pcr test for detection of 24 ocular infectious disease pathogens. | to establish and evaluate a new multiplex solid-phase strip polymerase chain reaction (strip pcr) for concurrent detection of common ocular infectious disease pathogens. | 2017 | 28282487 |
| prospective etiological investigation of community-acquired pulmonary infections in hospitalized people living with hiv. | the study of the etiological agents of community-acquired pulmonary infections is important to guide empirical therapy, requires constant updating, and has a substantial impact on the prognosis of patients. the objective of this study is to determine prospectively the etiology of community-acquired pulmonary infections in hospitalized adults living with hiv. patients were submitted to an extended microbiological investigation that included molecular methods. the microbiological findings were eva ... | 2017 | 28121925 |
| peptide 19 of porphyromonas gingivalis heat shock protein is a potent inducer of low-density lipoprotein oxidation. | although periodontal pathogens show a strong association with development of atherosclerosis, little is known about how a microorganism contributes to disease onset and progression. oxidation of low-density lipoprotein (ldl) is a major risk factor of atherogenesis. the principal objective of this study is to evaluate the ability of peptide 19 (pep19) of porphyromonas gingivalis (pg) heat shock protein (hsp) as a potent inducer of ldl oxidation, and a secondary objective is to compare this abilit ... | 2017 | 27712463 |
| chemical characterization and evaluation of antibacterial, antifungal, antimycobacterial, and cytotoxic activities of talinum paniculatum. | in this study, the bioactivity of talinum paniculatum was evaluated, a plant widely used in folk medicine. the extract from the t. paniculatum leaves (le) was obtained by percolation with ethanol-water and then subjecting it to liquid-liquid partitions, yielding hexane (hx), ethyl acetate (etoac), butanol (buoh), and aqueous (aq) fractions. screening for antimicrobial activity of the le and its fractions was evaluated in vitro through broth microdilution method, against thirteen pathogenic and n ... | 2017 | 26603226 |
| antimicrobial and cytotoxicity properties of the organic solvent fractions of clerodendrum myricoides (hochst.) r. br. ex vatke: kenyan traditional medicinal plant. | clerodendrum myricoides is a kenyan herbal plant used in the management of respiratory diseases. in the current study, we investigated in vitro antimicrobial activity, cytotoxicity, and phytochemical screening of c. myricoides. | 2017 | 27366347 |
| novel quinoxalinyl chalcone hybrid scaffolds as enoyl acp reductase inhibitors: synthesis, molecular docking and biological evaluation. | we report herein, first ever synthesis of series of novel differently substituted quinoxalinyl chalcones using claisen schmidt condensation, its molecular docking studies, and potential to be good anti-microbial, anti-tubercular and anti-cancer agents. the antimicrobial studies were carried out against staphylococcus aureus, escherichia coli and candida albicans using disc diffusion procedure. the selected chalcones were tested for anti-cancer and cytotoxicity activity against mcf-7 cancer cell ... | 2017 | 28372908 |
| antibiotic capture by bacterial lipocalins uncovers an extracellular mechanism of intrinsic antibiotic resistance. | the potential for microbes to overcome antibiotics of different classes before they reach bacterial cells is largely unexplored. here we show that a soluble bacterial lipocalin produced by burkholderia cenocepacia upon exposure to sublethal antibiotic concentrations increases resistance to diverse antibiotics in vitro and in vivo these phenotypes were recapitulated by heterologous expression in b. cenocepacia of lipocalin genes from pseudomonas aeruginosa, mycobacterium tuberculosis, and methici ... | 2017 | 28292982 |
| drivers of airborne human-to-human pathogen transmission. | airborne pathogens - either transmitted via aerosol or droplets - include a wide variety of highly infectious and dangerous microbes such as variola virus, measles virus, influenza a viruses, mycobacterium tuberculosis, streptococcus pneumoniae, and bordetella pertussis. emerging zoonotic pathogens, for example, mers coronavirus, avian influenza viruses, coxiella, and francisella, would have pandemic potential were they to acquire efficient human-to-human transmissibility. here, we synthesize in ... | 2017 | 27918958 |
| usefulness of lymphoid granulomatous inflammation culture obtained by endobronchial ultrasound-guided transbronchial needle aspiration in a fungal endemic area. | endobronchial ultrasound-guided transbronchial needle aspiration (ebus-tbna) is the procedure of choice for the evaluation of mediastinal/hilar lymph node enlargements. granulomatous inflammation of the mediastinal/hilar lymph nodes is often identified on routine histology. in addition, mediastinal lymphadenopathy may be present with undiagnosed infection. we sought to determine the usefulness of routine cultures and histology for infectious etiologies in a fungal endemic area when granulomatous ... | 2017 | 27503156 |
| an efficient system for deletion of large dna fragments in escherichia coli via introduction of both cas9 and the non-homologous end joining system from mycobacterium smegmatis. | accompanied with the internal non-homologous end joining (nhej) system, cas9 can be used to easily inactivate a gene or delete a fragment through introduction of dna double-stranded breaks (dsbs) in eukaryotic cells. while in most prokaryotes (e.g. escherichia coli), due to the lack of nhej, homologous recombination (hr) is required for repair of dsbs, which is less convenient. here, a markerless system was developed for rapid gene inactivation or fragment deletion in e. coli via introduction of ... | 2017 | 28257845 |
| madurastatin b3, a rare aziridine derivative from actinomycete nocardiopsis sp. ls150010 with potent anti-tuberculosis activity. | since the discovery of the first antibiotic, natural products have played an important role in chemistry, biology and medicine. to explore the potential of bioactive compounds from microbes isolated from the southeast of tibet, china, a crude extract library was constructed and screened against staphylococcus aureus. the strain nocardiopsis sp. ls150010 was scaled up and subjected to further chemical studies, resulting in the identification of n-salicyloyl-2-aminopropan-1,3-diol (2) and its rare ... | 2017 | 28181080 |
| development of antibacterial conjugates using sulfamethoxazole with monocyclic terpenes: a systematic medicinal chemistry based computational approach. | to develop 6 conjugate agents of the moribund antibiotic sulfamethoxazole (smz) joined to 6 individual monoterpenes, followed by protocols of medicinal chemistry as potent antibacterials, against multidrug resistant (mdr) human gruesome pathogenic bacteria. | 2017 | 28254074 |
| bacterial virulence factors: secreted for survival. | virulence is described as an ability of an organism to infect the host and cause a disease. virulence factors are the molecules that assist the bacterium colonize the host at the cellular level. these factors are either secretory, membrane associated or cytosolic in nature. the cytosolic factors facilitate the bacterium to undergo quick adaptive-metabolic, physiological and morphological shifts. the membrane associated virulence factors aid the bacterium in adhesion and evasion of the host cell. ... | 2017 | 28148975 |
| model systems for pulmonary infectious diseases: paradigms of anthrax and tuberculosis. | robert koch utilized animal model systems to put forward his postulates while discovering the etiological agents of anthrax and tuberculosis, bacillus anthracis and mycobacterium tuberculosis, respectively. after more than 130 years, we have achieved limited success towards understanding these two pestilences, which have propagated as scourge against humans. b. anthracis and m. tuberculosis are diverse organisms, which share a common evolutionary path in tropics. they adapt unique strategies to ... | 2017 | 28137237 |
| antimycobacterial, antimicrobial activity, experimental (ft-ir, ft-raman, nmr, uv-vis, dsc) and dft (transition state, chemical reactivity, nbo, nlo) studies on pyrrole-isonicotinyl hydrazine. | as part of a study of pyrrole hydrazone, we have investigated quantum chemical calculations, molecular geometry, relative energy, vibrational properties and antimycobacterial/antimicrobial activity of pyrrole-2-carboxaldehyde isonicotinyl hydrazone (pcinh), by applying the density functional theory (dft) and hartree fock (hf). good reproduction of experimental values is obtained and with small percentage error in majority of the cases in comparison to theoretical result (dft). the experimental f ... | 2017 | 28213139 |
| novel bacterial topoisomerase inhibitors with potent broad-spectrum activity against drug-resistant bacteria. | the novel bacterial topoisomerase inhibitor class is an investigational type of antibacterial inhibitor of dna gyrase and topoisomerase iv that do not have cross-resistance with the quinolones. here, we report the evaluation of the in vitro properties of a new series of this type of small molecules. exemplar compounds selectively and potently inhibited the catalytic activities of escherichia coli dna gyrase and topoisomerase iv but did not block the dna breakage-reunion step. compounds showed br ... | 2017 | 28223393 |
| the antibacterial activities of aditoprim and its efficacy in the treatment of swine streptococcosis. | aditoprim (adp) has potential use as an antimicrobial agent in animals. however, its pharmacodynamic properties have not been systematically studied yet. in this study, the in vitro antibacterial activities of adp and its main metabolites were assayed, and the in vivo antibacterial efficacy of adp for the treatment of swine streptococcosis was evaluated. it was shown that salmonella and streptococcus from swine, escherichia coli and salmonella from chickens, e. coli, streptococcus, mannheimia, p ... | 2017 | 28145487 |
| pathogenic mechanisms of intracellular bacteria. | we wished to overview recent data on a subset of epigenetic changes elicited by intracellular bacteria in human cells. reprogramming the gene expression pattern of various host cells may facilitate bacterial growth, survival, and spread. | 2017 | 28134679 |
| the role of universal stress proteins in bacterial infections. | universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ... | 2017 | 28120710 |
| refugees and antimicrobial resistance: a systematic review. | there is a large increase in the numbers of refugees and asylum seekers worldwide and a lack of data on the carriage of antimicrobial resistance in refugee/asylum seeking groups. | 2017 | 27919742 |
| rational design of reversible inhibitors for trehalose 6-phosphate phosphatases. | in some organisms, environmental stress triggers trehalose biosynthesis that is catalyzed collectively by trehalose 6-phosphate synthase, and trehalose 6-phosphate phosphatase (t6pp). t6pp catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to trehalose and inorganic phosphate and is a promising target for the development of antibacterial, antifungal and antihelminthic therapeutics. herein, we report the design, synthesis and evaluation of a library of aryl d-glucopyranoside 6-sulfates to se ... | 2017 | 28192710 |
| reoxidation of the thiol-disulfide oxidoreductase mdba by a bacterial vitamin k epoxide reductase in the biofilm-forming actinobacterium actinomyces oris. | post-translocational protein folding in the gram-positive biofilm-forming actinobacterium actinomyces oris is mediated by a membrane-bound thiol-disulfide oxidoreductase named mdba, which catalyzes oxidative folding of nascent polypeptides transported by the sec translocon. reoxidation of mdba involves a bacterial vitamin k epoxide reductase (vkor)-like protein that contains four cysteine residues c93/c101 and c175/c178 with the latter forming a canonical cxxc thioredoxin-like motif; however, th ... | 2017 | 28289087 |
| chemical characteristics, antimicrobial and cytotoxic activities of the essential oil of egyptian cinnamomum glanduliferum bark. | the essential oil isolated from the bark of cinnamomum glanduliferum (wall) meissn that grown in egypt was screened for the first time. the chemical composition was analyzed by gc and gc/mass. the antimicrobial activity of the oil was assessed using agar-well diffusion method toward representatives for each of gram-positive bacteria, gram-negative bacteria, and fungi. the cytotoxic activity was checked using three human cancer cell lines. twenty seven compounds were identified, representing 99.0 ... | 2017 | 28190283 |
| facing the challenges of multiscale modelling of bacterial and fungal pathogen-host interactions. | recent and rapidly evolving progress on high-throughput measurement techniques and computational performance has led to the emergence of new disciplines, such as systems medicine and translational systems biology. at the core of these disciplines lies the desire to produce multiscale models: mathematical models that integrate multiple scales of biological organization, ranging from molecular, cellular and tissue models to organ, whole-organism and population scale models. using such models, hypo ... | 2017 | 26857943 |
| differential roles of iron storage proteins in maintaining the iron homeostasis in mycobacterium tuberculosis. | ferritins and bacterioferritins are iron storage proteins that represent key players in iron homeostasis. several organisms possess both forms of ferritins, however, their relative physiological roles are less understood. mycobacterium tuberculosis possesses both ferritin (bfrb) and bacterioferritin (bfra), playing an essential role in its pathogenesis as reported by us earlier. this study provides insights into the role of these two proteins in iron homeostasis by employing m. tuberculosis bfr ... | 2017 | 28060867 |
| targeting drug-sensitive and -resistant strains of mycobacterium tuberculosis by inhibition of src family kinases lowers disease burden and pathology. | in view of emerging drug resistance among bacterial pathogens, including mycobacterium tuberculosis, the development of novel therapeutic strategies is increasingly being sought. a recent paradigm in antituberculosis (anti-tb) drug development is to target the host molecules that are crucial for intracellular survival of the pathogen. we previously showed the importance of src tyrosine kinases in mycobacterial pathogenesis. here, we report that inhibition of src significantly reduced survival of ... | 2017 | 27303736 |
| epidemiology of rifampicin resistant tuberculosis and common mutations in rpob gene of mycobacterium tuberculosis: a retrospective study from six districts of punjab (india) using xpert mtb/rif assay. | xpert mtb/rif assay has revolutionized the diagnosis of tuberculosis (tb) by simultaneously detecting the bacteria and resistance to rifampicin (rif), a surrogate marker for multidrug-resistant tb (mdr-tb) in <2 h. the rif resistance pattern in malwa region of punjab, india, is not documented. here, we report the epidemiology of rif-resistant tb and mutations in rpob gene of mycobacterium tuberculosis (mtb). | 2017 | 27365918 |
| real-time imaging of mycobacterium tuberculosis, using a novel near-infrared fluorescent substrate. | slow growth of mycobacterium tuberculosis, the causative agent of tuberculosis, hinders advancement in all areas of research toward prevention and treatment. real-time imaging with reporter enzyme fluorescence (ref) that uses custom fluorogenic substrates for bacterial enzymes allows rapid and specific detection of m. tuberculosis in live animals. we have synthesized a novel ref substrate, cnir800, that carries a near-infrared (nir) fluorochrome irdye 800cw, with a quencher connected through the ... | 2017 | 27421748 |
| bronchoscopic diagnostic procedures and microbiological examinations in proving endobronchial tuberculosis. | to determine the proportional distribution of endobronchial tuberculosis (ebtb) subtypes and to evaluate the types of bronchoscopic diagnostic procedures that can prove granulomatous inflammation. | 2017 | 27383932 |
| 3d correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy. | the processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. here, we describe a workflow for 3d correlative light and electron microscopy (clem) of cell monolayers using fluorescence microscopy to identify and follow biological events, combined with serial blockface scanning electron microscopy to analyse the underlying ultrastructure. the workflow encompasses all steps from cell culture to sample processing, imaging strategy, and ... | 2017 | 27445312 |
| significance of immune response to mycobacterium tuberculosis culture filtrate protein antigens in cerebrospinal fluid of tuberculous meningitis patients: a search for diagnostic marker. | mycobacterium tuberculosis (h37ra) culture filtrate proteins (cfp) are explored as a diagnostic marker for tuberculous meningitis (tbm). cerebrospinal fluid (csf) samples from patients were categorized as confirmed (n = 47), suspected (n = 20), and non-tbm (n = 25) cases. immune response by western blot revealed tbm csf samples are having heterogeneous response to cfp. cfp elisa was 92% sensitive and 38.30% specific. ods of confirmed tbm and non-tbm cases were significantly different (p < 0.0001 ... | 2017 | 27454259 |
| evaluation of laboratory diagnosis for cutaneous tuberculosis. | cutaneous tuberculosis (ctb) is still difficult to diagnose due to its varied clinical presentation and limitations of diagnostic methods. the aim of this study was to evaluate the results of diagnostic laboratory tests available for ctb. | 2017 | 27510659 |
| comparison of quantiferon-tb gold in tube test versus tuberculin skin test for screening of latent tuberculosis infection in saudi arabia: a population-based study. | to compare quantiferon-tb gold in tube (qft-git) test with tuberculin skin test (tst) in detecting latent tuberculosis infection (ltbi) among a general population in saudi arabia. | 2017 | 27512509 |
| immune activation by mycobacterium tuberculosis in hiv-infected and -uninfected subjects. | this study investigates the influence of mycobacterium tuberculosis infection on immune activation biomarkers, both in hiv-infected and -uninfected subjects. | 2017 | 27532475 |
| computational elucidation of structural basis for ligand binding with mycobacterium tuberculosis glucose-1-phosphate thymidylyltransferase (rmla). | glucose-1-phosphate thymidylyltransferase (rmla) is one of the enzymes in rhamnose biosynthesis pathway, where rhamnose acts as linker of peptidoglycan and arabinogalacton in the cell wall, therefore rmla is a potential enzyme for the survival of mycobacterium tuberculosis (mtb). to go into the depth of the structure for exploring binding regions, homology model of rmla was built in prime, schrodinger v9.2. the model with lowest discrete optimized potential energy (dope) score of -35524.17 kcal/ ... | 2017 | 27633891 |
| multifocal tuberculous osteomyelitis in a 3-year-old child. | multifocal skeletal tuberculosis accounts for less than 1% of all tuberculous lesions in children. a 3-year-old unimmunised child presented with painless symmetrical swelling of both arms, pallor and hepatosplenomegaly. a skeletal survey demonstrated lytic lesions of the metadiaphysis of both humeri, a rib and the sacrum. the possibility of langerhans cell histiocytosis or multifocal fibrous dysplasia was considered; however, acid-fast bacilli were detected in the biopsy specimen and real-time p ... | 2017 | 27458046 |
| loop-mediated isothermal amplification assay for detection of mycobacterium tuberculosis complex in infertile women. | female genital tuberculosis (fgtb) has a profound impact on the reproductive health of patients including infertility. conventional diagnostic techniques have low sensitivity and specificity as well as long turnaround time. there is a need of developing newer, rapid and practically adaptable technique, especially in low-income countries. | 2017 | 27514954 |
| characterization of myma protein as a flavin-containing monooxygenase and as a target of isoniazid. | tuberculosis is a global health problem especially with the emergence of drug-resistant mycobacterium tuberculosis strains, creating an urgent need to identify new drug targets. the mycobacterial cell wall is an attractive target for chemotherapeutic agents. gene products of myma operon are known to be required for the maintenance of cell wall and play an important role in persistence, thus making them important drug targets. this study was undertaken to biochemically characterize the myma as a ... | 2017 | 27539124 |
| an easy and sensitive sandwich assay for detection of mycobacterium tuberculosis ag85b antigen using quantum dots and gold nanorods. | mycobacterium tuberculosis is a serious global infectious pathogen causing tuberculosis (tb). the development of an easy and sensitive method for the detection of m. tuberculosis is in urgent need due to complex and low specificity of the current assays. herein, we present a novel method for m. tuberculosis detection based on a sandwich assay via antigen-antibody interaction using silica-coated quantum dots (siqds) and gold nanorods (aunrs). a genetically engineered recombinant antibody (gbp-50b ... | 2017 | 27551994 |
| enolase of mycobacterium tuberculosis is a surface exposed plasminogen binding protein. | enolase, a glycolytic enzyme, has long been studied as an anchorless protein present on the surface of many pathogenic bacteria that aids in tissue remodeling and invasion by binding to host plasminogen. | 2017 | 27569900 |
| human cd40 ligand deficiency dysregulates the macrophage transcriptome causing functional defects that are improved by exogenous ifn-γ. | cd40 ligand (cd40l) deficiency predisposes to opportunistic infections, including those caused by fungi and intracellular bacteria. studies of cd40l-deficient patients reveal the critical role of cd40l-cd40 interaction for the function of t, b, and dendritic cells. however, the consequences of cd40l deficiency on macrophage function remain to be investigated. | 2017 | 27554817 |
| subunit vaccine h56/caf01 induces a population of circulating cd4 t cells that traffic into the mycobacterium tuberculosis-infected lung. | the capacity of cd4 t cells to protect against mycobacterium tuberculosis (mtb) is governed by their ability to localize to the lung site of infection. subunit vaccine h56/caf01, a liposome-adjuvanted fusion protein of mtb antigens ag85b, esat-6, and rv2660, conferred durable protection and elicited polyfunctional cd4 t cells that preferentially localized to the lung parenchyma. these lung-resident t cells had reduced klrg1 and increased cxcr3 expression, an intermediate state of th1 differentia ... | 2017 | 27554293 |
| diagnostic potential of multi-targeted lamp (loop-mediated isothermal amplification) for osteoarticular tuberculosis. | delay in diagnosing osteoarticular tuberculosis (oatb) contributes significantly to morbidity by causing disfiguration and neurological sequelae. the delay caused by conventional culture and the expertise and expense involved in other nucleic acid based tests, make lamp (loop-mediated isothermal amplification) assay a favorable middle path. we evaluated lamp assay using is6110 and mpb64 for rapid diagnosis of oatb by comparing with is6110 pcr and culture. lamp assay was performed on 140 synovial ... | 2017 | 27175946 |
| lppm impact on the colonization of macrophages by mycobacterium tuberculosis. | mycobacterium tuberculosis produces several bacterial effectors impacting the colonization of phagocytes. here, we report that the putative lipoprotein lppm hinders phagocytosis by macrophages in a toll-like receptor 2-dependent manner. moreover, recombinant lppm is able to functionally complement the phenotype of the mutant, when exogenously added during macrophage infection. lppm is also implicated in the phagosomal maturation, as a lppm deletion mutant is more easily addressed towards the aci ... | 2017 | 27220037 |
| molecular analysis of idiopathic subglottic stenosis for mycobacterium species. | idiopathic subglottic stenosis (isgs) is an unexplained obstruction involving the lower laryngeal and upper tracheal airway. persistent mucosal inflammation is a hallmark of the disease. epithelial microbiota dysbiosis is found in other chronic inflammatory mucosal diseases; however, the relationship between tracheal microbiota composition and isgs is unknown. given the critical role for host defense at mucosal barriers, we analyzed tissue specimens from isgs patients for the presence of microbi ... | 2017 | 27295947 |
| young adult hip: reactivation of dormant, previously undiagnosed mycobacterium tuberculosis infection following intra-articular steroid injection. | tuberculosis (tb) still remains a common problem in the uk and, with the increasing number of patients being offered arthroplasties; periprosthetic involvement is not uncommon anymore. however, the diagnosis of tb infected arthroplasties still remains difficult and misdiagnosis is common, therefore delaying treatment. | 2017 | 27299119 |
| proteomic analysis of drug-resistant mycobacterium tuberculosis by one-dimensional gel electrophoresis and charge chromatography. | multidrug-resistant tuberculosis (mdr-tb) is a form of tb caused by mycobacterium tuberculosis (m. tuberculosis) that do not respond to, at least, isoniazid and rifampicin, the two most powerful, first-line (or standard) anti-tb drugs. novel intervention strategies for eliminating this disease were based on finding proteins that can be used for designing new drugs or new and reliable kits for diagnosis. the aim of this study was to compare the protein profiles of mdr-tb with sensitive isolates. ... | 2017 | 27417316 |
| structure and dynamics of the multi-domain resuscitation promoting factor rpfb from mycobacterium tuberculosis. | rpfb is multidomain protein that is crucial for mycobacterium tuberculosis resuscitation from dormancy. this protein cleaves cell wall peptidoglycan, an essential bacterial cell wall polymer formed by glycan chains of β-(1-4)-linked-n-acetylglucosamine (glcnac) and n-acetylmuramic acid (murnac) cross-linked by short peptide stems. rpfb is structurally complex being composed of five distinct domains, namely a catalytic, a g5 and three duf348 domains. here, we have undertaken a combined experiment ... | 2017 | 27420638 |
| mycobacteria and autophagy: many questions and few answers. | tuberculosis (tb) is an ancient disease caused by mycobacterium tuberculosis (mtb). tb is one of the world's deadliest diseases, with one-third of infected individuals falling ill each year especially in many developing countries. upon invading host cells, such as macrophages, mtb can replicate in infected cells by arresting phagosome maturation and then potentially escaping into the cytosol. host cells have a mechanism to control intracellular mtb by inducing autophagy, which is an elaborate ce ... | 2017 | 27443861 |
| nontuberculous mycobacteria among pulmonary tuberculosis patients: a retrospective belgian multicenter study. | currently, there are no european data about the frequency and clinical significance of nontuberculous mycobacteria (ntm) grown from respiratory samples during the treatment of tuberculosis (tb). we determined the frequency and clinical significance of ntm isolated before or during pulmonary tuberculosis treatment in belgian laboratories. | 2017 | 27345031 |
| review on toll-like receptor activation in myasthenia gravis: application to the development of new experimental models. | abnormal toll-like receptor (tlr) activation and uncontrolled resolution of inflammation are suspected to play a key role in the development of autoimmune diseases. acquired myasthenia gravis (mg) is an invalidating neuromuscular disease leading to muscle weaknesses. mg is mainly mediated by anti-acetylcholine receptor (achr) autoantibodies, and thymic hyperplasia characterized by ectopic germinal centers is a common feature in mg. an abnormal expression of certain tlrs is observed in the thymus ... | 2017 | 27207173 |
| perturbation of genome integrity to fight pathogenic microorganisms. | resistance against antibiotics is unfortunately still a major biomedical challenge for a wide range of pathogens responsible for potentially fatal diseases. | 2017 | 27217086 |
| screening for streptomycin resistance conferring mutations in mycobacterium tuberculosis isolates from iran. | point mutations in the rpsl and rrs genes can lead to development of streptomycin (str) resistance in mycobacterium tuberculosis. the aims of this study were to determine the frequency of mutations in str resistant m. tuberculosis isolates in iran and to analyze the possible relationship between bacterial genotype and str resistance. twenty-three m. tuberculosis samples comprising 9 multidrug-resistant (mdr) and 14 non-mdr isolates, recovered from tb patients in four regions: tehran (n = 14), is ... | 2017 | 27380108 |
| correction: locked nucleic acid probe-based real-time pcr assay for the rapid detection of rifampin-resistant mycobacterium tuberculosis. | [this corrects the article doi: 10.1371/journal.pone.0143444.]. | 2017 | 27271433 |
| [resistance to second-line anti-tuberculosis drugs among peruvian multidrug resistant mycobacterium tuberculosis strains]. | to determine the drug resistance profiles for quinolones: ciprofloxacin (cfx), ofloxacin (ofx), moxifloxacin (mfx), and gatifloxacin (gfx); and for injectables: kanamycin (kan), amikacin (amk), and capreomycin (cap) in multidrug resistant (mdr) strains. we also investigated the correlation between mutations in rrs, tlya and gyra/b genes, and the in vitro resistance to the second-line anti-tuberculosis drugs. | 2017 | 25597717 |
| effect of lagerstroemia tomentosa and diospyros virginiana methanolic extracts on different drug-resistant strains of mycobacterium tuberculosis. | mycobacterium tuberculosis (mtb) is the causative agent of tuberculosis. the increasing incidence of multi drug resistance tuberculosis (mdr-tb) and extensively drug resistance tuberculosis (xdr-tb) worldwide highlighted the urgent need to search for alternative antimycobacterial agents. more and more people in developing countries utilize traditional medicine for their major primary health care needs. it has been determined that pharmaceutical plant, lagerstroemia tomentosa and diospyros virgin ... | 2017 | 25657789 |
| surgical management of recalcitrant peripheral bronchopleural fistula with empyema: a preliminary experience. | peripheral bronchopleural fistula (bpf) and empyema from necrotising infections of the lung and pleural is difficult to treat resulting in increased morbidity and mortality rates. the aim of this study was to show the effectiveness of the latissimus dorsi muscle (ldm) flap and patch closure techniques in the management of recalcitrant peripheral bpfs with the aid of thoracotomy. | 2017 | 25657487 |
| recombinant bacille calmette-guerin coexpressing ag85b, cfp10, and interleukin-12 elicits effective protection against mycobacterium tuberculosis. | the tuberculosis (tb) pandemic remains a leading cause of human morbidity and mortality, despite widespread use of the only licensed anti-tb vaccine, bacille calmette-guerin (bcg). the protective efficacy of bcg in preventing pulmonary tb is highly variable; therefore, an effective new vaccine is urgently required. | 2017 | 25732698 |
| elbow mycobacterium tuberculosis in america. | 2017 | 25722623 | |
| evaluation of geno type mtbdrplus line probe assay for early detection of drug resistance in tuberculous meningitis patients in india. | molecular methods which allow for rapid and reliable detection of drug resistance have yet not been sufficiently evaluated for timely management of patients with tuberculous meningitis. | 2017 | 25722613 |
| serological follow-up of tuberculosis in a wild boar population in contact with infected cattle. | there is an increasing concern in several european countries over the role that tuberculosis (tb)-infected wild boar may play in the progress of bovine tb eradication campaigns. in 2004, as a consequence of the detection of a tb focus in wild boar from a national game reserve (ngr) located in southern catalonia, a surveillance programme based on post-mortem inspection for detection of macroscopic tb-like lesions (tbll) was initiated in the affected area. the source of infection for wild boar was ... | 2017 | 25944524 |
| mycobacterium tuberculosis: 2014 clinical trials in review. | 2017 | 25798148 | |
| first-line anti-tubercular drug resistance of mycobacterial strains from re-treatment cases that were smear-positive at 4(th) month onwards under the revised national tuberculosis control program. | programmatic management of drug-resistant tb (pmdt) under the rntcp is being implemented in west bengal in a phased manner since 2011. during the initial years mdr-tb cases were identified based on criteria a. this study examines the first line anti-tubercular drug resistance pattern of mycobacteria cultured from sputum samples of mdr suspects who were retreatment cases smear positive from 4(th) month onwards. | 2017 | 25814796 |
| contact investigations around mycobacterium tuberculosis patients without positive respiratory culture. | to evaluate the yield and effectiveness of contact investigations conducted around potentially infectious tuberculosis (tb) patients with no positive respiratory culture for mycobacterium tuberculosis in new york city (nyc). | 2017 | 25867495 |
| synthesis and biological activities of some benzimidazolone derivatives. | the reaction of 5-nitrobenzimidazolone with phenoxyethyl bromide in presence of potassium carbonate in dimethyl formamide obtained 6-nitro-1,3-bis(2-phenoxyethyl)-1,3-dihydro-2h-benzimidazol-2-one. it was reduced using stannous chloride to get 6-amino -1,3-bis(2-phenoxyethyl)-1, 3-dihydro-2h-benzimidazol -2-one, which was further treated with aromatic sulphonyl chloride to obtain benzimidazolone derivatives, 6a-k. these compounds were tested for antibacterial, antituberculosis and antifungal act ... | 2017 | 26009659 |
| the factors associated with tuberculosis recurrence in the northwest and west of iran. | identifying the factors associated with the recurrence of mycobacterium tuberculosis (mtb) can help in tuberculosis disease prevention and reducing the burden on the health care system. this study was designed to identify the factors associated with recurrence in mtb patients in five border provinces of iran. | 2017 | 25897280 |
| tuberculous osteomyelitis of the maxilla: a rarest of rare case report. | tuberculosis is a chronic granulomatous systemic infectious disease caused by mycobacterium tuberculosis. the oral lesions found in tuberculosis are relatively rare and may present as ulcers, erythematous patches, indurated lesions, nodules or as bony jaw lesions. oral tubercular lesions sometimes present a confusing clinical presentation and can be overlooked. hence, we document a case of tuberculous osteomyelitis of the maxilla in a 19-year-old female patient, who was initially treated for mul ... | 2017 | 25937732 |
| concurrent central nervous system and endobronchial tuberculosis mimicking a metastatic lung cancer. | early diagnosis and treatment of central nervous system tuberculosis are important because of high mortality and morbidity. airways must be carefully evaluated in differential diagnosis. | 2017 | 25919475 |
| diffuse miliary nodules in the bronchial mucosa observed by bronchoscopy: sarcoidosis or tuberculosis? | miliary tuberculosis is a form of tuberculosis that is characterized by a wide dissemination into lung fields. so far, no report has shown a miliary tuberculosis case where diffuse miliary nodules can be observed in airways by bronchoscopy. here we reported a case of military tuberculosis that can easily be misdiagnosed as sarcoidosis. | 2017 | 25918882 |
| rifapentine-proliposomes for inhalation: in vitro and in vivo toxicity. | oral therapy for pulmonary tuberculosis (tb) treatment suffers from the limitation of hepatic metabolism leading insufficient concentration of antitubercular (anti-tb) drugs in alveolar macrophage which harbors mycobacterium tuberculosis (mtb). targeted aerosol delivery of antituberculous drug to lung is efficient for treating local lung tb infection. | 2017 | 25948966 |
| microarray analysis of the in vitro granulomatous response to mycobacterium tuberculosis h37ra. | the hallmark of tuberculosis is the granuloma, an organized cellular accumulation playing a key role in host defense against mycobacterium tuberculosis. these structures sequester and contain mycobacterial cells preventing active disease, while long term maintenance of granulomas leads to latent disease. clear understanding on mechanisms involved in granuloma formation and maintenance is lacking. | 2017 | 26019382 |
| tubercular meningitis and lymphadenitis mimicking a relapse of burkitt's lymphoma on (18)f-fdg-pet/ct: a case report. | tuberculosis (tb) can present with various forms and can occasionally be mistaken for malignancy. hereby, we report a 53-year-old man diagnosed and treated for burkitt's lymphoma in 2009 who achieved a complete remission confirmed by a computed tomography (ct) scan. during the follow-up 2 years later, he complained of left hip pain that warranted investigation with magnetic resonance imaging and whole-body (18)f-fludeoxyglucose-positron emission tomography (fdg-pet)/ct which showed a benign lesi ... | 2017 | 26078742 |
| differential transcriptomic and metabolic profiles of m. africanum- and m. tuberculosis-infected patients after, but not before, drug treatment. | the epidemiology of mycobacterium tuberculosis (mtb) and m. africanum (maf) suggests differences in their virulence, but the host immune profile to better understand the pathogenesis of tuberculosis (tb) have not been studied. we compared the transcriptomic and metabolic profiles between mtb- and maf-infected tb cases to identify host biomarkers associated with lineages-specific pathogenesis and response to anti-tb chemotherapy. venous blood samples from mtb- and maf-infected patients obtained b ... | 2017 | 26043170 |
| utility of mpt64 antigen detection for rapid confirmation of mycobacterium tuberculosis complex. | rapid differentiation of the mycobacterium tuberculosis complex (mtbc) and mycobacteria other than tuberculosis (mott) is crucial to facilitate early and effective treatment of the patients. clinical presentation of mtbc and mott is not always very clear and routine conventional methods are time consuming. | 2017 | 26069425 |
| amperometric immunosensor for rapid detection of mycobacterium tuberculosis. | tuberculosis (tb) has been a major public health problem, which can be better controlled by using accurate and rapid diagnosis in low-resource settings. a simple, portable, and sensitive detection method is required for point-of-care (poc) settings. this paper studies an amperometric biosensor using a microtip immunoassay for a rapid and low cost detection of mycobacterium tuberculosis (mtb) in sputum. mtb in sputum is specifically captured on the functionalized microtip surface and detected by ... | 2017 | 26097292 |
| pattern of drug resistance of mycobacterium tuberculosis clinical isolates to first-line antituberculosis drugs in pulmonary cases. | mycobacterium tuberculosis (mtb), the human pathogen causes tuberculosis (tb). it is a highly infectious and globally pandemic disease. the severity increases when the mtb becomes resistant to antituberculosis drugs. india is reported to be in the second place, with the highest number of drug-resistant tb cases. the treatment of drug-resistant tb is even more complicated. | 2017 | 26180382 |
| formulation and characterization of pyrazinamide polymeric nanoparticles for pulmonary tuberculosis: efficiency for alveolar macrophage targeting. | pyrazinamide, a highly specific agent against mycobacterium tuberculosis is used as first-line drug to treat tuberculosis. the current work aims to formulate polymeric nanoparticles based drug delivery system to sustain the release profile and reduce the dosing frequency of pyrazinamide. further aim was to target the macrophages within body fluid. these polymeric nanoparticles were prepared by simultaneous double-emulsion (w/o/w) solvent evaporation/diffusion technique. the prepared dispersions ... | 2017 | 26180270 |
| multiple skin abscesses and myofibrosis of bilateral lower limbs following repeated intramuscular injection of pentazocine with concomitant tuberculous infection. | prescription drug abuse is a major health problem across the globe. various complications associated with repeated injection of pentazocine are reported in the literature, including skin fibrosis, skin abscesses and ulceration, abnormal skin pigmentation and fibrous myopathy. | 2017 | 26146644 |
| recent advances in the diagnosis and treatment of childhood tuberculosis. | despite over 2.3 million (26% of global burden) cases of tuberculosis (tb) in india the accurate diagnosis of childhood tb remains a major challenge. children with tb usually have paucibacillary disease and contribute little to disease transmission within the community. consequently the treatment of children with tb is often not considered a priority by tb control programmes. adequate and timely assessment of tb infection in childhood could diminish epidemiological burden as underdiagnosed pedia ... | 2017 | 26283820 |
| the role of laboratory investigations in evaluating abdominal tuberculosis. | tuberculosis (tb) continues to be a major health problem in developing countries like india. abdominal tb is defined as an infection of the peritoneum, or hollow or solid abdominal organs with mycobacterium tuberculosis (mtb). the gastrointestinal tract is one of the most frequent sites of extrapulmonary involvement in tb. the present study was undertaken to evaluate the role of laboratory investigations in the diagnosis of abdominal tb. | 2017 | 26392795 |
| a case of tuberculous granulomatous panniculitis without vasculitis. | we report a case of tuberculous granulomatous panniculitis without vasculitis in an 87-year-old female patient with b-cell chronic lymphocytic leukaemia. one month after starting chemotherapy with chlorambucil and prednisone she presented superficial erythematous plaques on the anterior side of the left leg. three weeks later erythematous painless deep nodules appeared on the left popliteal fossa and on the left thigh. cutaneous biopsy revealed granulomatous panniculitis without caseation necros ... | 2017 | 26351420 |
| 5-arylaminouracil derivatives as potential dual-action agents. | several 5-aminouracil derivatives that have previously been shown to inhibit mycobacterium tuberculosis growth at concentrations of 5-40 μg/ml are demonstrated to act also as noncompetitive non-nucleoside inhibitors of hiv-1 reverse transcriptase without causing toxicity in vitro (mt-4 cells) and ex vivo (human tonsillar tissue). | 2017 | 26483967 |
| dormancy models for mycobacterium tuberculosis: a minireview. | dormancy models for mycobacterium tuberculosis play important roles in understanding various aspects of tuberculosis pathogenesis and in the testing of novel therapeutic regimens. by simulating the latent tuberculosis infection, in which the bacteria exist in a non-replicative state, the models demonstrate reduced susceptibility to antimycobacterial agents. this minireview outlines the models available for simulating latent tuberculosis both in vitro and in several animal species. additionally, ... | 2017 | 26413043 |
| a model of shared mycobacteriology testing services: lessons learned. | the need for public health laboratories (phls) to prioritize resources has led to increased interest in sharing diagnostic services. to address this concept for tuberculosis (tb) testing, the new york state department of health wadsworth center and the rhode island state health laboratories assessed the feasibility of shared services for the detection and characterization of mycobacterium tuberculosis complex (mtbc). | 2017 | 26556934 |
| representativeness of tuberculosis genotyping surveillance in the united states, 2009-2010. | genotyping of mycobacterium tuberculosis isolates contributes to tuberculosis (tb) control through detection of possible outbreaks. however, 20% of u.s. cases do not have an isolate for testing, and 10% of cases with isolates do not have a genotype reported. tb outbreaks in populations with incomplete genotyping data might be missed by genotyping-based outbreak detection. therefore, we assessed the representativeness of tb genotyping data by comparing characteristics of cases reported during jan ... | 2017 | 26556930 |
| relevance of semen polymerase chain reaction positive for tuberculosis in asymptomatic men undergoing infertility evaluation. | male partners of infertile women with genital tuberculosis (tb) are often screened for genital tb. we aimed to evaluate the clinical significance of a positive screening semen polymerase chain reaction (pcr) for mycobacterium tuberculosis test (tb-pcr) in asymptomatic men undergoing infertility evaluation and determine the need for a detailed investigation and treatment for tb. | 2017 | 26538860 |
| molecular epidemiology of mycobacterium tuberculosis strains in the north-west and west of iran. | identifying mycobacterium tuberculosis (mtb) transmission type is a key step in the control of this disease. | 2017 | 26500790 |