Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| progress in the surveillance of respiratory syncytial virus (rsv) in europe: 2001-2008. | respiratory syncytial virus (rsv) surveillance is important to get insight into the burden of disease and epidemic pattern of rsv infection. this information is useful for healthcare resource allocation as well as the timing of preventive messages and palivizumab prophylaxis. for influenza surveillance the european influenza surveillance scheme (eiss) was established in 1996, but no surveillance platform is available for rsv. to improve surveillance an rsv task group was established in 2003 and ... | 2009 | 19822120 |
| detection and hazard assessment of pathogenic microorganisms in medical wastes. | this study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. medical wastes collected from 5 major hospitals in south korea were classified and stored at three different temperatures (-20, 6, and 30 degrees c). presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and b ... | 2009 | 19827491 |
| [construction of minireplicon of human respiratory syncytial virus]. | the reverse genetics technology is an important way to develop genetically engineered attenuated living human respiratory syncytial virus (rsv) vaccine candidates. as the pilot experiment,it is necessary to prepare rsv minireplicon and investigate its biological activity. | 2009 | 19835166 |
| novel therapies for an old virus: treatment of rsv infections in the 21st century. | respiratory syncytial virus (rsv) is a pathogen whose existence has been known for decades, causing mild-to-severe upper and lower respiratory tract infections that bear the risk of subsequent asthma and can even lead to a fatal outcome. rsv infects all groups of patients and is a major cause of hospitalization in children and in the elderly. this review briefly summarizes the current status of rsv drug development and clinical trials for drugs available for the treatment of rsv infections. | 2009 | 19883332 |
| antigen-based assays for the identification of influenza virus and respiratory syncytial virus: why and how to use them in pediatric practice. | this article describes the clinical and socioeconomic relevance of influenza (iv) and respiratory syncytial virus (rsv) in pediatrics, the characteristics and limitations of currently available assays, and the impact of rapid diagnostic tests. this article shows that rapid tests for the detection and identification of iv and rsv in the respiratory secretions of infants and children are useful in the diagnosis of common, and possibly severe diseases, such as influenza and bronchiolitis. the tests ... | 2009 | 19892226 |
| respiratory syncytial virus vaccine development. | respiratory syncytial virus (rsv) is a clinically significant cause of respiratory tract disease, especially among high-risk infants and immunocompromised and elderly adults. despite the burden of disease, there is no licensed prophylactic rsv vaccine. the initial efforts to develop an rsv vaccine involved formalin-inactivated virus preparations that unexpectedly caused vaccine-enhanced disease in clinical trials in rsv-naive children. over the last 40 years, cautious and deliberate progress has ... | 2009 | 19892231 |
| [human metapneumovirus (hmpv) infections in young children]. | human metapneumovirus (hmpv) is a recently discovered respiratory virus. hmpv, the second after respiratory syncytial virus (rsv), is leading cause of upper and lower respiratory tract infections mainly in infants and children. hmpv infections have been reported in many countries in all the world, in patients in every age and gender, but children under 5 years are most likely to be susceptible to infections caused by hmpv. the frequency of hmpv infections require hospitalization in this group is ... | 2009 | 19899593 |
| the cost effectiveness of palivizumab in term inuit infants in the eastern canadian arctic. | canadian, inuit, full term infants have the highest rate of respiratory syncytial virus (rsv) infection globally, which results in substantial costs associated hospitalisation. | 2009 | 19900071 |
| late preterm infants and risk for rsv. | late preterm infants, born between 34 and 36 6/7 weeks gestation, are physiologically immature and at risk for a variety of complications including infection caused by respiratory syncytial virus (rsv). rsv infection that spreads to the lower respiratory tract results in hospitalization of these high-risk infants, where nurses provide nursing care focusing on suctioning, maintaining fluid balance, temperature control, and oxygenation. this article describes the risk and incidence of rsv infectio ... | 2009 | 19901700 |
| impairment of t cell immunity by the respiratory syncytial virus: targeting virulence mechanisms for therapy and prophylaxis. | worldwide, respiratory syncytial virus (rsv) causes severe bronchiolitis and pneumonia in children, the elderly and immuno-compromised individuals. moreover, rsv is the mayor cause of infant hospitalization due to lower respiratory infection, regardless socioeconomic status. accumulating data support the notion that immune responses elicited against naturally acquired rsv infections are non-lasting and inappropriate for efficient virus clearance. although there is consensus over the capacity of ... | 2009 | 19903147 |
| [investigation of respiratory syncytial virus by three different methods in children with lower respiratory tract infection]. | respiratory syncytial virus (rsv) is the most important viral agent leading to lower respiratory tract infection in infants and children. the aim of this study was to investigate the presence of rsv by direct immunofluorescence antibody (dfa), cell culture and polymerase chain reaction (pcr) in children with lower respiratory tract infection. nasotracheal aspirate specimens collected from 80 hospitalized patients aged between 0-24 months and clinically diagnosed as lower respiratory tract infect ... | 2009 | 19795618 |
| [the role of serum leptin in infants with wheezing after respiratory syncytial virus infected]. | to determine the role of serum leptin in infants with wheezing after respiratory syncytial virus infected. | 2009 | 19799014 |
| [transferability of trial results into clinical practice: examples taken from paediatric practice]. | since 2000 there has been sufficient evidence that chest x-rays are unnecessary in infants and children with uncomplicated lower respiratory tract infection. the reason is that neither the diagnosis nor the first therapeutic decisions will be influenced by the result of this procedure, and especially so if children have been infected with the respiratory syncytial virus. however, epidemiological studies in germany reveal an ongoing use of chest x-ray in these cases. this might suggest that the x ... | 2009 | 19839210 |
| [association of tumor necrosis factor-alpha-308g/a and interleukin-6-174g/c gene polymorphisms with the susceptibility of respiratory syncytial virus bronchiolitis]. | to study the association of tumor necrosis factor (tnf)-alpha-308g/a and interleukin (il)-6-174g/c gene polymorphisms with the susceptibility of respiratory syncytial virus (rsv) bronchiolitis. | 2009 | 19849941 |
| [detection of respiratory syncytial virus in children with respiratory tract infections by nucleic acid amplification fluorogenic quantitative assay]. | nucleic acid amplification (pcr) fluorogenic quantitative assay is used for the diagnosis of respiratory syncytial virus (rsv) infection. this study was designed to explore the sensitivity of pcr fluorogenic quantitative assay for ascertaining respiratory rsv infection and rsv infection conditions by detecting the presence of rsv-rna related sequences in children. | 2009 | 19849942 |
| respiratory syncytial virus infection facilitates acute colonization of pseudomonas aeruginosa in mice. | pseudomonas aeruginosa causes opportunistic infections in immunocompromised individuals and patients ventilated mechanically and is the major pathogen in patients with cystic fibrosis, in which it causes chronic infections. epidemiological, in vitro and animal data suggest a role for respiratory virus infections in facilitating colonization and infection with p. aeruginosa. a study was undertaken to determine whether respiratory syncytial virus (rsv) infection could facilitate the initiation of ... | 2009 | 19856469 |
| serum kl-6 levels as a biomarker of lung injury in respiratory syncytial virus bronchiolitis. | to evaluate whether kl-6 concentration is a useful biomarker of the severity of respiratory syncytial virus (rsv) bronchiolitis, we determined kl-6 concentrations in patients with rsv bronchiolitis with or without chronic heart disease (chd). we enrolled 52 patients who had been diagnosed with rsv bronchiolitis and required admission to the hospital at the department of pediatrics of fukushima medical university school of medicine from 2004 to 2005. these patients were divided into two groups: g ... | 2009 | 19856476 |
| potent high-affinity antibodies for treatment and prophylaxis of respiratory syncytial virus derived from b cells of infected patients. | native human abs represent attractive drug candidates; however, the low frequency of b cells expressing high-quality abs has posed a barrier to discovery. using a novel single-cell phenotyping technology, we have overcome this barrier to discover human abs targeting the conserved but poorly immunogenic central motif of respiratory syncytial virus (rsv) g protein. for the entire cohort of 24 subjects with recent rsv infection, b cells producing abs meeting these stringent specificity criteria wer ... | 2009 | 19841167 |
| pediatric obstructive sleep apnea: a potential late consequence of respiratory syncitial virus bronchiolitis. | to examine the hypothesis that children who suffered from severe respiratory syncitial virus (rsv) bronchiolitis during infancy may be at higher risk for obstructive sleep apnea (osa) later in childhood. | 2009 | 19911395 |
| il-9 regulates pathology during primary and memory responses to respiratory syncytial virus infection. | il-9 is a cytokine of great current interest associated with allergic/th2 responses. high levels of il-9 are present in bronchial secretions from infants with respiratory syncytial virus (rsv) bronchiolitis. to test its effects in rsv disease with a th2 profile, balb/c mice were vaccinated with recombinant vaccinia virus expressing the rsv g protein. on rsv challenge, immunized mice developed augmented disease characterized by enhanced pulmonary th2 and local il-9 production peaking on days 7-10 ... | 2009 | 19915054 |
| respiratory syncytial virus risk factors in late preterm infants. | the key role of respiratory syncytial virus (rsv) in causing infant morbidity and hospitalizations is worldwide well recognized. the late preterm infants (34-36 weeks of gestational age (wga)) showed a higher risk of hospitalization for rsv-induced infection as compared with full-term infants and similar to that seen in very preterm infants. in addition to the prematurity, a number of risk factors have been identified in 33-35 wga infants that are associated with rsv-hospitalization as demonstra ... | 2009 | 19925369 |
| relative impact of influenza and respiratory syncytial virus in young children. | we measured the relative impact of influenza and respiratory syncytial virus (rsv) infections in young children in terms of emergency department (ed) visits, clinical care requirements, and overall resource use. | 2009 | 19933730 |
| respiratory viruses in hospitalized children with acute lower respiratory tract infections in harbin, china. | this study investigated the prevalence of respiratory viruses, including respiratory syncytial virus (rsv), influenza virus types a and b (flu a/b), parainfluenza virus (para) 1-3, and adenovirus (ad), in hospitalized children with acute lower respiratory tract infections (alris). immunofluorescence assays identified viral etiology in 412 patients younger than 16 years old. the overall viral isolation rate was 63.1% (260/412). the rsv was detected in 25.0%, flu a/b in 19.4%, para 1-3 in 14.6%, a ... | 2009 | 19934539 |
| respiratory syncytial virus infection in recipients of allogeneic stem-cell transplantation: a retrospective study of the incidence, clinical features, and outcome. | respiratory syncytial virus (rsv) is a common cause of serious respiratory infections in hematopoietic stem-cell transplant (hsct) recipients. we aimed to determine the frequency, risk factors, and outcome of rsv infection in allo-hsct recipients. | 2009 | 19935377 |
| compliance with rsv prophylaxis: global physicians' perspectives. | respiratory syncytial virus (rsv) is a significant cause of morbidity in high-risk infants. palivizumab is proven to prevent serious rsv disease, but compliance with prophylaxis (monthly doses during the rsv season) is essential to ensure protection. we invited 453 pediatricians to participate in a survey to identify their perspectives of barriers to compliance and interventions to improve compliance with palivizumab prophylaxis schedules. one hundred physicians from five continents completed th ... | 2009 | 19936162 |
| evaluation of alternative respiratory syndromes for specific syndromic surveillance of influenza and respiratory syncytial virus: a time series analysis. | syndromic surveillance is increasingly being evaluated for its potential for early warning of increased disease activity in the population. however, interpretation is hampered by the difficulty of attributing a causative pathogen. we described the temporal relationship between laboratory counts of influenza and respiratory syncytial virus (rsv) detection and alternative groupings of emergency department (ed) respiratory diagnoses. | 2009 | 19943970 |
| [the burden and outcomes of acute bronchiolitis among young children hospitalized in israel]. | acute bronchiolitis (ab) is a significant indication for hospitalization during the winter period. underlying conditions increase risk for severe manifestations. | 2009 | 20027974 |
| the level and duration of rsv-specific maternal igg in infants in kilifi kenya. | respiratory syncytial virus (rsv) is the major cause of lower respiratory tract infection in infants. the rate of decay of rsv-specific maternal antibodies (rsv-matab), the factors affecting cord blood levels, and the relationship between these levels and protection from infection are poorly defined. | 2009 | 19956576 |
| genetics, recombination and clinical features of human rhinovirus species c (hrv-c) infections; interactions of hrv-c with other respiratory viruses. | to estimate the frequency, molecular epidemiological and clinical associations of infection with the newly described species c variants of human rhinoviruses (hrv), 3243 diagnostic respiratory samples referred for diagnostic testing in edinburgh were screened using a vp4-encoding region-based selective polymerase chain reaction (pcr) for hrv-c along with parallel pcr testing for 13 other respiratory viruses. hrv-c was the third most frequently detected behind respiratory syncytial virus (rsv) an ... | 2009 | 20041158 |
| the burden of respiratory syncytial virus bronchiolitis on a pediatric inpatient service. | respiratory syncytial virus bronchiolitis is the single leading cause of pediatric admissions for infants in the first year of life, presenting regularly in epidemic proportions in the winter months and impacting in major way on pediatric inpatient services. | 2009 | 19960846 |
| [viral etiology of acute respiratory infections]. | acute respiratory infections (ari) are pathologies that affect the respiratory tract from the proximal pharynx to the lungs, with an evolution of less than 15 days. they constitute the most frequent cause of morbimortality in the world. with the purpose of identifying the viral agents associated to this type of infections in patients of zulia state, venezuela, between february 2005 and july 2006, a total of 102 samples of the respiratory tract (oropharyngeal swab, nasopharyngeal swab, nasopharyn ... | 2009 | 19961058 |
| crystal structure of a nucleocapsid-like nucleoprotein-rna complex of respiratory syncytial virus. | the respiratory syncytial virus (rsv) is an important human pathogen, yet neither a vaccine nor effective therapies are available to treat infection. to help elucidate the replication mechanism of this rna virus, we determined the three-dimensional (3d) crystal structure at 3.3 a resolution of a decameric, annular ribonucleoprotein complex of the rsv nucleoprotein (n) bound to rna. this complex mimics one turn of the viral helical nucleocapsid complex, which serves as template for viral rna synt ... | 2009 | 19965480 |
| [transcription factors in spinal dorsal root ganglion in rats recurrently infected with respiratory syncytial virus]. | to elucidate intracellular transcription factor activation of c(7) approximately t(5) dorsal root ganglia in rats recurrently infected with respiratory syncytial virus (rsv). | 2009 | 20045913 |
| [effect of an active component from forsythia suspensa (thunb.) vahl against respiratory syncytial virus in vitro]. | to study the extraction of an active component lc-4 from forsythia suspensa (thunb.) vahl and anti-respiratory syncytial virus (rsv) effect in vitro. | 2009 | 20047236 |
| nanoparticle vaccines against respiratory viruses. | influenza virus, respiratory syncytial virus (rsv), and parainfluenza type 3 virus (pi-3v) are the major viral agents which are consistently involved in causing lower respiratory tract disease in humans and animals. the virus infection begins in the upper respiratory tract, where immune responses are initiated, and then progresses to the lower respiratory tract where destruction of cells and tissues leads to bronchitis, bronchiolitis, and pneumonia, which is occasionally fatal. nanoparticle vacc ... | 2009 | 20049806 |
| motavizumab. | motavizumab (medi-524, numax) is a second generation monoclonal antibody (mab) derived from palivizumab (synagis) using affinity maturation techniques. motavizumab is currently undergoing us food and drug administration review as a treatment for respiratory syncytial virus (rsv) prophylaxis. it has been evaluated in large-scale clinical studies, and has demonstrated efficacy in reducing the disease burden of rsv in high-risk infant populations. | 2009 | 20065632 |
| [isolation and identification of avian leukosis virus-b from layer chickens infected with avian leukosis virus-j]. | two strains of avian leukosis virus subgroup b (alv-b) were isolated for the first time in china hy-line white on the cultured df-1 cells which were inoculated tissue samples from by an elisa assay, a histopathology examination and a pcr-based diagnosis. the results from the elisa assay indicated that the positive rate of serum antibodies to alv-b and alv-j virus were 16.3% (15/92) and 13% (12/92), respectively. the histopathological examination indicated that two types of tumor cells existed at ... | 2009 | 20077935 |
| [factors related to recurrent wheezing after hospitalization with rsv infection with the children who were aged three years old or younger: a questionnaire survey]. | respiratory syncytial virus (rsv) infections in infancy have been related to the subsequent recurrent wheezing and asthma. however, there are a few reports about the relationship between rsv infection and subsequent wheezing in japan. we sought to determine the contributing factors for wheezing illness after rsv infection in 99 japanese patients with rsv-associated hospitalizations by questionnaire and follow up survey. fifty eight patients, who were aged three years old or younger on admission ... | 2009 | 20168069 |
| anti-virus effect of traditional chinese medicine yi-fu-qing granule on acute respiratory tract infections. | yi-fu-qing granule is a traditional chinese medicine for the treatment of acute respiratory tract infections. the present study sought to investigate the anti-virus effects of yi-fu-qing granule on acute respiratory infections with respiratory syncytial virus (rsv) and human adenoviruses type 3 (ad3). the cytotoxicity of yi-fu-qing granule was evaluated by the neutral red assay on hela cells. the antiviral effect of yi-fu-qing granule was tested by observing the cytopathogenic effect (cpe) with ... | 2009 | 20103834 |
| variation of respiratory syncytial virus and the relation with meteorological factors in different winter seasons. | respiratory syncytial virus (rsv) is the most important viral agent causing severe respiratory disease in infants and children. in temperate climates, rsv activity typically peaks during winter. we have described the seasonal variation in rsv activity and investigated which meteorological variables are related to rsv outbreaks for different time lags. | 2009 | 20118684 |
| the role of toll-like receptors in regulating the immune response against respiratory syncytial virus. | toll-like receptors (tlrs) play a distinct role in battling respiratory syncytial virus (rsv) infections. however, due to a lack of representative animal models and several early controversies, the field is unclear. in this systematic review, we have elucidated conflicting results and outlined important factors that might affect study outcomes. we reviewed studies that used different doses/viral strains, performed virus propagation in different cell lines, or used different mice strains. the fol ... | 2009 | 20121698 |
| [an etiological study on acute respiratory infection among inpatient children in suzhou]. | to investigate the pathogen of children with acute respiratory infection (ari) in suzhou and to provide some evidences for clinical diagnosis and treatment. | 2009 | 20137465 |
| [effects of respiratory syncytial virus upon expressions of mrna and protein of intercellular adhesion molecule-1 in human embryonic lung fibroblast cells]. | to explore the effects of respiratory syncytial virus (rsv) upon the expressions of mrna and protein of intercellular adhesion molecule-1 (icam-1) in human embryonic lung fibroblast cells. | 2009 | 20137698 |
| [human metapneumovirus]. | the human metapneumovirus (hmpv) is a new pneumovirinae related to the avian metapneumovirus type c. hmpv genome differs from human respiratory syncytial virus (rsv) genome by the gene order and the lack of nonstructural genes. two genetic sub-groups and four sub-types of hmpv are identified. hmpv infections evolve as regular winter outbreaks which have roughly the same size and overlaping rsv epidemics. among hospitalized children in caen, hmpv is detected in 9.7% of the cases after rsv (37%), ... | 2009 | 18515017 |
| pre-existing disease is associated with a significantly higher risk of death in severe respiratory syncytial virus infection. | 600,000 deaths worldwide are estimated to be directly or indirectly attributable to respiratory syncytial virus (rsv). | 2009 | 18653625 |
| infant exposure to fine particulate matter and traffic and risk of hospitalization for rsv bronchiolitis in a region with lower ambient air pollution. | few studies investigate the impact of air pollution on the leading cause of infant morbidity, acute bronchiolitis. we investigated the influence of pm(2.5) and other metrics of traffic-derived air pollution exposure using a matched case-control dataset derived from 1997 to 2003 birth and infant hospitalization records from the puget sound region, washington state. mean daily pm(2.5) exposure for 7, 30, 60 and lifetime days before case bronchiolitis hospitalization date were derived from communit ... | 2009 | 19211100 |
| a chimeric a2 strain of respiratory syncytial virus (rsv) with the fusion protein of rsv strain line 19 exhibits enhanced viral load, mucus, and airway dysfunction. | respiratory syncytial virus (rsv) is the leading cause of respiratory failure and viral death in infants. abundant airway mucus contributes to airway obstruction in rsv disease. interleukin-13 (il-13) is a mediator of pulmonary mucus secretion. it has been shown that infection of balb/c mice with the rsv line 19 strain but not with the rsv a2 laboratory strain results in lung il-13 and mucus expression. here, we sequenced the rsv line 19 genome and compared it to the commonly used a2 and long st ... | 2009 | 19211758 |
| school age outcome of hospitalisation with respiratory syncytial virus infection of prematurely born infants. | hospitalisation due to respiratory syncytial virus (rsv) infection in the first 2 years after birth has been associated with increased healthcare utilisation and associated costs up to 5 years of age in children born prematurely at less than 32 weeks of gestation who developed bronchopulmonary dysplasia (bpd). a study was undertaken to determine whether hospitalisation due to rsv infection in the first 2 years was associated with increased morbidity and lung function abnormalities in such childr ... | 2009 | 19213770 |
| micronutrient concentrations in respiratory syncytial virus and human metapneumovirus in yemeni children. | acute respiratory infections (ari) cause significant childhood mortality. nutritional homeostasis, particularly micronutrient levels, is important in modulating response to infection. more information is required regarding micronutrient levels in ari viral infections, especially newly identified viruses such as human metapneumovirus (hmpv). | 2009 | 19222932 |
| foreign glycoproteins can be actively recruited to virus assembly sites during pseudotyping. | retroviruses like human immunodeficiency virus type 1 (hiv-1), as well as many other enveloped viruses, can efficiently produce infectious virus in the absence of their own surface glycoprotein if a suitable glycoprotein from a foreign virus is expressed in the same cell. this process of complementation, known as pseudotyping, often can occur even when the glycoprotein is from an unrelated virus. although pseudotyping is widely used for engineering chimeric viruses, it has remained unknown wheth ... | 2009 | 19224995 |
| recognizing the importance of respiratory syncytial virus in chronic obstructive pulmonary disease. | acute exacerbations of chronic obstructive pulmonary disease (copd) are responsible for a large proportion of the health care dollar expenditure, morbidity, and mortality related to copd. respiratory infections are the most common cause of acute exacerbations, but recent evidence indicates that the importance of respiratory syncytial virus (rsv) infection in copd is under-appreciated. improved detection of rsv using techniques based on the polymerase chain reaction accounts for much of the incre ... | 2009 | 19229710 |
| respiratory syncytial virus: from primary care to critical care. | respiratory syncytial virus (rsv) is a common disease in pediatrics. certain subpopulations of children are at greatest risk for serious disease. however, previously healthy children also may become critically ill. in the clinic or the intensive care unit, children with rsv pose the challenge of how to treat a disease when evidence to support therapeutic options is severely limited. prophylaxis is an option for certain children, although many do not qualify. rsv has been implicated in continued ... | 2009 | 19232926 |
| intranasal immunization with a replication-deficient adenoviral vector expressing the fusion glycoprotein of respiratory syncytial virus elicits protective immunity in balb/c mice. | human respiratory syncytial virus (rsv) is a serious pediatric pathogen of the lower respiratory tract worldwide. there is currently no clinically approved vaccine against rsv infection. recently, it has been shown that a replication-deficient first generation adenoviral vector (fgad), which encodes modified rsv attachment glycoprotein (g), elicits long-term protective immunity against rsv infection in mice. the major problem in developing such a vaccine is that g protein lacks mhc-i-restricted ... | 2009 | 19233131 |
| murine host responses to respiratory syncytial virus (rsv) following intranasal administration of a protollin-adjuvanted, epitope-enhanced recombinant g protein vaccine. | immunization of mice with the g protein of respiratory syncytial virus (rsv) characteristically induces an immune response that is partially protective, but which can prime for pulmonary eosinophilia. we have shown previously that the n191a mutation in a recombinant rsv g protein fragment is associated with reduced pulmonary eosinophilic infiltration when administered with alum subcutaneously in balb/c mice followed by rsv challenge. we hypothesize that the performance of this "epitope enhanced" ... | 2009 | 19233722 |
| fas ligand is required for the development of respiratory syncytial virus vaccine-enhanced disease. | children immunized with a formalin-inactivated respiratory syncytial virus (rsv) vaccine experienced enhanced disease and exhibited pulmonary eosinophilia upon natural rsv infection. balb/c mice immunized with either formalin-inactivated rsv or a recombinant vaccinia virus (vacv) expressing the rsv attachment (g) protein develop extensive pulmonary eosinophilia after rsv challenge that mimics the eosinophilic response observed in the children during the 1960s vaccine trials. fas ligand (fasl) is ... | 2009 | 19234198 |
| subversion of pulmonary dendritic cell function by paramyxovirus infections. | lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hmpv) and respiratory syncytial virus (rsv) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the ag-presenting function of the lung which follow these infections. in this study, we investigated whether hmpv and rsv experimental infections alter the phenotype and function of dendritic cell (dc) subsets that are re ... | 2009 | 19234204 |
| epidemiological and clinical study of viral respiratory tract infections in children from italy. | impact of recently discovered viruses on epidemiology of acute respiratory tract infections (arti) is still unclear. we studied the impact of recently discovered human metapneumovirus (hmpv), human bocavirus (hbov), and new coronaviruses (hcov-nl63 and hku1) on the global epidemiology of arti. from october 2006 to april 2007, 237 pediatric patients affected by arti were enrolled in our study. specimens were tested for respiratory viruses by polymerase chain reaction. one hundred twenty-four out ... | 2009 | 19235872 |
| human metapneumovirus infections--biannual epidemics and clinical findings in children in the region of basel, switzerland. | human metapneumovirus (hmpv) epidemics vary in time and severity. we report findings for pcr for hmpv and respiratory syncytial virus (rsv) performed on nasopharyngeal aspirates (npa) of hospitalized and outpatient children with respiratory tract infections between october 2004 and april 2008. a total of 3,934 npas were tested for hmpv and 3,859 for rsv. of these, 198 (5%) were hmpv positive and 869 (23%) were rsv-positive. median age was 17 months and 9 months for hmpv and rsv, respectively. fi ... | 2009 | 19238433 |
| in very young infants severity of acute bronchiolitis depends on carried viruses. | rt amplification reaction has revealed that various single viruses or viral co-infections caused acute bronchiolitis in infants, and rv appeared to have a growing involvement in early respiratory diseases. because remaining controversial, the objective was to determine prospectively the respective role of rsv, rv, hmpv and co-infections on the severity of acute bronchiolitis in very young infants. | 2009 | 19240806 |
| characterization and subcellular localization of an rna silencing suppressor encoded by rice stripe tenuivirus. | rice stripe virus (rsv) is a single-stranded (ss) rna virus belonging to the genus tenuivirus. rsv is present in many east asian countries and causes severe diseases in rice fields, especially in china. in this study, we analyzed six proteins encoded by the virus for their abilities to suppress rna silencing in plant using a green fluorescent protein (gfp)-based transient expression assay. our results indicate that ns3 encoded by rsv rna3, but not other five rsv encoded proteins, can strongly su ... | 2009 | 19251298 |
| surface features of a mononegavirales matrix protein indicate sites of membrane interaction. | the matrix protein (m) of respiratory syncytial virus (rsv), the prototype viral member of the pneumovirinae (family paramyxoviridae, order mononegavirales), has been crystallized and the structure determined to a resolution of 1.6 a. the structure comprises 2 compact beta-rich domains connected by a relatively unstructured linker region. due to the high degree of side-chain order in the structure, an extensive contiguous area of positive surface charge covering approximately 600 a(2) can be res ... | 2009 | 19251668 |
| integrin alphavbeta1 promotes infection by human metapneumovirus. | human metapneumovirus (hmpv) is a recently described paramyxovirus that causes lower respiratory infections in children and adults worldwide. the hmpv fusion (f) protein is a membrane-anchored glycoprotein and major protective antigen. all hmpv f protein sequences determined to date contain an arg-gly-asp (rgd) sequence, suggesting that f engages rgd-binding integrins to mediate cell entry. the divalent cation chelator edta, which disrupts heterodimeric integrin interactions, inhibits infectivit ... | 2009 | 19164533 |
| an unusual respiratory syncytial virus nosocomial outbreak in an adult psychiatry ward. | herein we report our experience in containing an outbreak of nosocomial respiratory syncytial virus (rsv) infection in a psychiatric ward in central taiwan during a non-widespread rsv seasonal occurrence. a total of 8 patients and 4 healthcare workers in the psychiatric ward developed febrile illness or upper respiratory tract infection symptoms between august 23 and 29, 2005. rsv was identified by either viral culture or reverse transcriptase-polymerase chain reaction (rt-pcr) assay. rsv was is ... | 2009 | 19168963 |
| delayed exposure to infections and childhood lymphomas: a case-control study. | delayed exposure to common infections during childhood, have been implied to cause strong immunological response to a single infectious agent that eventually triggers leukemogenesis. the aim of the present study was to investigate whether decreased exposure to infections, as reflected in a more seronegative spectrum to several common infectious agents, is associated with increased risk for the development of childhood lymphomas. | 2009 | 19169895 |
| evidence that selective changes in the lipid composition of raft-membranes occur during respiratory syncytial virus infection. | we examined the structure of lipid-raft membranes in respiratory syncytial virus infected cells. cholesterol depletion studies using methyl-beta-cyclodextrin suggested that membrane cholesterol was required for virus filament formation, but not inclusion bodies. in addition, virus filament formation coincided with elevated 3-hydroxy-3-methylglutaryl-coenzyme a reductase expression, suggesting an increase in requirement for endogenous cholesterol synthesis during virus assembly. lipid raft membra ... | 2009 | 19178924 |
| comparison of cytokine responses in nasopharyngeal aspirates from children with viral lower respiratory tract infections. | to determine whether nasopharyngeal aspirates (npas) cytokine response is different according to the causative viruses in children with lower respiratory tract infections (lrti). | 2009 | 19183120 |
| respiratory syncytial virus outbreak in a long-term care facility detected using reverse transcriptase polymerase chain reaction: an argument for real-time detection methods. | to report an outbreak of respiratory synctyial virus (rsv) in a long-term care facility (ltcf) during ongoing routine respiratory illness surveillance. | 2009 | 19187415 |
| gene profiling studies in the neonatal ovine lung show enhancing effects of vegf on the immune response. | preterm and young neonates have an increased predisposition to respiratory distress syndrome (rds) associated with an immature development of lung surfactant. glucocorticoids (gcs) are the major immunomodulatory agents used to increase lung development and reduce the mortality and morbidity of preterm infants with rds. however, their safety remains uncertain, and the precise mechanisms by which they improve lung function are unclear. in previous studies, we found that vascular endothelial growth ... | 2009 | 19189846 |
| establishing a surveillance network for severe lower respiratory tract infections in korean infants and young children. | to reduce morbidity and mortality through integrated case management, a pilot study to detect respiratory viruses in patients with acute lower respiratory infections (alris) was designed as part of a nationwide surveillance for this disease in korea. the study population consisted of hospitalized patients under the age of 5 years with bronchiolitis, pneumonia, croup, or acute respiratory distress syndrome. a prospective 6-month study was performed. two hundred and ninety-seven nasopharyngeal sec ... | 2009 | 19190941 |
| human respiratory syncytial virus nonstructural protein ns2 antagonizes the activation of beta interferon transcription by interacting with rig-i. | a wide variety of rna viruses have been shown to produce proteins that inhibit interferon (ifn) production and signaling. for human respiratory syncytial virus (rsv), the nonstructural ns1 and ns2 proteins have been shown to block ifn signaling by causing the proteasomal degradation of stat2. in addition, recombinant rsvs lacking either ns1 or ns2 induce more ifn production than wild-type (wt) rsv in infected cells. however, the mechanisms by which the ns proteins perform this function are unkno ... | 2009 | 19193793 |
| mast cell degranulation is induced by a549 airway epithelial cell infected with respiratory syncytial virus. | respiratory syncytial virus (rsv), a major causative agent of respiratory tract infections, influences allergic diseases. mast cells, important effector cells in allergic disease, also express chemokine (c-x(3)-c motif) receptor 1 (cx(3)cr1). the rsv attachment glycoprotein (g protein) is structurally similar to cx(3)c ligand 1 (cx(3)cl1), the cx(3)cr1 ligand, suggesting that rsv directly interacts with and affects mast cell function, including degranulation. in this paper, the effect of rsv inf ... | 2009 | 19195674 |
| the burden of respiratory syncytial virus infection in young children. | the primary role of respiratory syncytial virus (rsv) in causing infant hospitalizations is well recognized, but the total burden of rsv infection among young children remains poorly defined. | 2009 | 19196675 |
| human piv-2 recombinant sendai virus (rsev) elicits durable immunity and combines with two additional rsevs to protect against hpiv-1, hpiv-2, hpiv-3, and rsv. | the human parainfluenza viruses (hpivs) and respiratory syncytial viruses (rsvs) are the leading causes of hospitalizations due to respiratory viral disease in infants and young children, but no vaccines are yet available. here we describe the use of recombinant sendai viruses (rsevs) as candidate vaccine vectors for these respiratory viruses in a cotton rat model. two new sendai virus (sev)-based hpiv-2 vaccine constructs were generated by inserting the fusion (f) gene or the hemagglutinin-neur ... | 2009 | 19200447 |
| optimization strategy to minimize wastage of palivizumab during the 2008 rsv season in são paulo, brazil. | palivizumab is currently recommended to high-risk children as a prophylaxis for respiratory syncytial virus (rsv) infection. however, it is still very expensive for developing countries like brazil. herein, we describe our strategy to minimize wastage of palivizumab during the 2008 rsv season. appointments were fixed for 304 children on 2 days of the week, so that a mean of 19.9 children received palivizumab per day. that allowed remaining volumes of palivizumab vials to be pooled and used for o ... | 2009 | 19203985 |
| pc4, a putative movement protein of rice stripe virus, interacts with a type i dnaj protein and a small hsp of rice. | rice stripe virus (rsv) infects rice and causes great yield reduction in some asian countries. in this study, rice cdna library was screened by a gal4-based yeast two-hybrid system using pc4, a putative movement protein of rsv, as the bait. a number of positive colonies were identified and sequence analysis revealed that they might correspond to ten independent proteins. two of them were selected and further characterized. the two proteins were a j protein and a small hsp, respectively. interact ... | 2009 | 19130198 |
| the causal direction in the association between respiratory syncytial virus hospitalization and asthma. | earlier studies have reported an increased risk of asthma after respiratory syncytial virus (rsv) hospitalization. other studies found that asthmatic disposition and propensity to wheeze increase the risk of rsv hospitalization. | 2009 | 19130934 |
| respiratory syncytial virus inhibits lung epithelial na+ channels by up-regulating inducible nitric-oxide synthase. | respiratory syncytial virus (rsv) infection has been shown to reduce na+-driven alveolar fluid clearance in balb/c mice in vivo. to investigate the cellular mechanisms by which rsv inhibits amiloride-sensitive epithelial na+ channels (enac), the main pathways through which na+ ions enter lung epithelial cells, we infected human clara-like lung (h441) cells with rsv that expresses green fluorescent protein (rra2). 3-6 days later patch clamp recordings showed that infected cells (i.e. cells expres ... | 2009 | 19131335 |
| genetic association study for rsv bronchiolitis in infancy at the 5q31 cytokine cluster. | the pathophysiological basis of severe respiratory syncytial virus (rsv) bronchiolitis in infancy is poorly understood and has hindered vaccine development. studies implicate the cell-mediated immune response in the pathogenesis of the disease. a recent twin study estimated a heritable contribution of 22% to rsv bronchiolitis. genetic epidemiology provides a new approach to identifying important immune determinants of disease severity. | 2009 | 19131452 |
| cigarette smoke alters respiratory syncytial virus-induced apoptosis and replication. | individuals exposed to cigarette smoke have a greater number and severity of viral infections, including respiratory syncytial virus (rsv) infections, than do nonsmokers, but the cellular mechanism is unknown. our objective was to determine the mechanism by which cigarette smoke augments viral infection. we hypothesize that cigarette smoke causes necrosis and prevents virus-induced cellular apoptosis, and that this is associated with increased inflammation and viral replication. primary airway e ... | 2009 | 19131644 |
| efficacy of oral ribavirin in lung transplant patients with respiratory syncytial virus lower respiratory tract infection. | respiratory syncytial virus (rsv) can cause severe lower respiratory tract infection (lri) and is a risk factor for the development of bronchiolitis obliterans syndrome (bos) after lung transplantation (ltx). currently, the most widely used therapy for rsv is inhaled ribavirin. however, this therapy is costly and cumbersome. we investigated the utility of using oral ribavirin for the treatment of rsv infection after ltx. | 2009 | 19134533 |
| lower levels of plasmacytoid dendritic cells in peripheral blood are associated with a diagnosis of asthma 6 yr after severe respiratory syncytial virus bronchiolitis. | plasmacytoid dendritic cells (pdc) play a crucial role in antiviral immunity and promoting th1 polarization, possibly protecting against development of allergic disease. examination of the relationship between peripheral blood plasmacytoid dc levels and manifestations of asthma and atopy early in life. we have isolated peripheral blood mononuclear cells (pbmc) from 73 children (mean age +/- sd: 6.6 +/- 0.5 yr old) participating in the rsv bronchiolitis in early life (rbel) study. flow cytometry ... | 2009 | 19140903 |
| comparison of viral isolation and multiplex real-time reverse transcription-pcr for confirmation of respiratory syncytial virus and influenza virus detection by antigen immunoassays. | we evaluated the prodesse proflu-1 real-time reverse transcription-pcr multiplex assay with the smartcycler instrument for the detection of human respiratory syncytial virus (rsv) and influenza a and b viruses in comparison to conventional cell culture and antigen immunoassays with the bd directigen a+b and binax now rsv assays over two successive respiratory virus seasons. ninety-two percent of the 361 specimens tested were nasopharyngeal aspirates obtained from individual patients, of which 11 ... | 2009 | 19129410 |
| dscg reduces rsv-induced illness in rsv-infected mice. | respiratory syncytial virus (rsv) is one of the pathogens generally associated with the common cold, lower respiratory infection, and exacerbation of asthma. disodium cromoglycate (dscg) is a safe and widely used drug for the prevention of bronchial asthma and allergic rhinitis attacks. the effect of dscg on acute upper respiratory tract viral infections remains controversial. the purpose of the study was to investigate the effects of dscg on parameters of rsv induced-illness. using a well-chara ... | 2009 | 19107959 |
| pulmonary v gamma 4+ gamma delta t cells have proinflammatory and antiviral effects in viral lung disease. | host defenses, while effecting viral clearance, contribute substantially to inflammation and disease. this double action is a substantial obstacle to the development of safe and effective vaccines against many agents, particularly respiratory syncytial virus (rsv). rsv is a common cold virus and the major cause of infantile bronchiolitis worldwide. the role of alphabeta t cells in rsv-driven immunopathology is well studied, but little is known about the role of "unconventional" t cells. during p ... | 2009 | 19124761 |
| temperature affects expression of symptoms induced by soybean mosaic virus in homozygous and heterozygous plants. | seven strains (g1 to g7) of soybean mosaic virus (smv) and 3 resistance loci (rsv1, rsv3, and rsv4) have been identified in soybean. the interaction of smv strains and host resistance genes results in resistant (symptomless), susceptible (mosaic), or necrotic (leaf and stem necrosis) reactions. the necrotic reaction may be gene dosage dependent and influenced by temperature. using a set of soybean isolines and hybrids containing homozygous or heterozygous alleles of rsv, rsv1, rsv1-n, rsv3, or r ... | 2009 | 19126638 |
| genetic diversity of the host and severe respiratory syncytial virus-induced lower respiratory tract infection. | respiratory syncytial virus (rsv)-induced lower respiratory tract disease is a common problem in children and adults in western societies. the clinical range of rsv infection from asymptomatic to respiratory distress syndrome is believed to be the outcome of viral and host immunity interactions. genes associated with immune response are of particular interest regarding genetic predisposition to severe rsv infection. several investigators have sought to identify genetic markers for high-risk pati ... | 2009 | 19106772 |
| respiratory syncytial virus neutralizing antibodies in cord blood, respiratory syncytial virus hospitalization, and recurrent wheeze. | respiratory syncytial virus (rsv) hospitalization is associated with wheeze. | 2009 | 19101023 |
| [lower respiratory tract infections with influenza and respiratory syncytial viruses in hospitalized elderly patients during the 2005-2006 winter season]. | to assess the proportion of lower respiratory tract infections (lrti) attributable to influenza virus and respiratory syncytial virus (rsv) during the 2005-2006 winter period, among hospitalized elderly in a geriatric unit of a french hospital near paris and and describe the characteristics of these infections. | 2009 | 19097850 |
| structural characterization of the rous sarcoma virus rna stability element. | in eukaryotic cells, an mrna bearing a premature termination codon (ptc) or an abnormally long 3' untranslated region (utr) is often degraded by the nonsense-mediated mrna decay (nmd) pathway. despite the presence of a 5- to 7-kb 3' utr, unspliced retroviral rna escapes this degradation. we previously identified the rous sarcoma virus (rsv) stability element (rse), an rna element downstream of the gag natural translation termination codon that prevents degradation of the unspliced viral rna. ins ... | 2009 | 19091866 |
| lack of antibody affinity maturation due to poor toll-like receptor stimulation leads to enhanced respiratory syncytial virus disease. | respiratory syncytial virus (rsv) is a leading cause of hospitalization in infants. a formalin-inactivated rsv vaccine was used to immunize children and elicited nonprotective, pathogenic antibody. immunized infants experienced increased morbidity after subsequent rsv exposure. no vaccine has been licensed since that time. a widely accepted hypothesis attributed the vaccine failure to formalin disruption of protective antigens. here we show that the lack of protection was not due to alterations ... | 2009 | 19079256 |
| excess drug prescriptions during influenza and rsv seasons in the netherlands: potential implications for extended influenza vaccination. | influenza and respiratory syncytial virus (rsv) infections are responsible for considerable morbidity, mortality and health-care resource use. for the netherlands, we estimated age and risk-group specific numbers of antibiotics, otologicals and cardiovascular prescriptions per 10,000 person-years during periods with elevated activity of influenza or rsv, and compared these with peri-season rates. data were taken from the university of groningen in-house prescription database (www.iadb.nl) and vi ... | 2009 | 19071185 |
| evaluation of nanogen mgb alert detection reagents in a multiplex real-time pcr for influenza virus types a and b and respiratory syncytial virus. | a multiplex real-time rt-pcr assay that detects influenza a, influenza b and respiratory syncytial virus (rsv) using the mgb alert influenza a&b/rsv detection reagent ruo (nanogen, san diego, ca) was developed. the nanogen detection reagents consist of pcr primers and minor groove binder-conjugated hybridization probes for each virus and an internal control. virus typing was determined by post-pcr melt curve analysis. a non-competitive armored rna internal control was co-extracted with each samp ... | 2009 | 19061916 |
| virus-specific ige enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice. | respiratory syncytial virus (rsv)-specific ige is a component of the host response to rsv infection, but its role in the subsequent enhancement of altered airway responsiveness is unknown. | 2009 | 19056111 |
| comparison of four nasal sampling methods for the detection of viral pathogens by rt-pcr-a ga(2)len project. | the aim of this study was to compare the efficacy and patient discomfort between four techniques for obtaining nasal secretions. nasal secretions from 58 patients with symptoms of a common cold, from three clinical centers (amsterdam, lodz, oslo), were obtained by four different methods: swab, aspirate, brush, and wash. in each patient all four sampling procedures were performed and patient discomfort was evaluated by a visual discomfort scale (scale 1-5) after each procedure. single pathogen rt ... | 2009 | 19041346 |
| association of respiratory virus activity and environmental factors with the incidence of invasive pneumococcal disease. | to correlate the incidence rate of invasive pneumococcal disease (ipd) with fluctuations in respiratory virus activity and environmental factors in christchurch, new zealand. | 2009 | 19042025 |
| identification of viral and atypical bacterial pathogens in children hospitalized with acute respiratory infections in hong kong by multiplex pcr assays. | acute respiratory tract infection is a leading cause of hospital admission of children. this study used a broad capture, rapid and sensitive method (multiplex pcr assay) to detect 20 different respiratory pathogens including influenza a subtypes h1, h3, and h5; influenza b; parainfluenza types 1, 2, 3, and 4; respiratory syncytial virus (rsv) groups a and b; adenoviruses; human rhinoviruses; enteroviruses; human metapneumoviruses; human coronaviruses oc43, 229e, and sars-cov; chlamydophila pneum ... | 2009 | 19031443 |
| respiratory syncytial virus infection in children admitted to hospital but ventilated mechanically for other reasons. | one thousand five hundred sixty-eight rsv infections were documented prospectively in 1,541 pediatric patients. of these, 20 (1.3%) had acquired the rsv infection while treated by mechanical ventilation for reasons other than the actual rsv infection (group ventilated mechanically). the clinical characteristics of children who were infected with respiratory syncytial virus (rsv) infection while ventilated mechanically for other reasons are described and compared with a matched control group. six ... | 2009 | 19031467 |
| respiratory syncytial virus activates innate immunity through toll-like receptor 2. | respiratory syncytial virus (rsv) is a common cause of infection that is associated with a range of respiratory illnesses, from common cold-like symptoms to serious lower respiratory tract illnesses such as pneumonia and bronchiolitis. rsv is the single most important cause of serious lower respiratory tract illness in children <1 year of age. host innate and acquired immune responses activated following rsv infection have been suspected to contribute to rsv disease. toll-like receptors (tlrs) a ... | 2009 | 19019963 |
| tlr4 and myd88 control protection and pulmonary granulocytic recruitment in a murine intranasal rsv immunization and challenge model. | an intranasal vaccine composed of toll-like receptor 2 (tlr2) ligand neisseria meningitidis outer membrane proteins and toll-like receptor 4 (tlr4) ligand shigella flexneri lipopolysaccharide (lps) (protollin) and enriched respiratory syncytial virus (rsv) proteins (ersv) has been demonstrated to promote balanced th1/th2 responses without eosinophil recruitment and to protect against challenge in mouse models. we used tlr2, tlr4 and myeloid differentiation factor 88 (myd88) knock-out (-/-) mice ... | 2009 | 19013492 |
| characterization of a persistent respiratory syncytial virus showing a low-fusogenic activity associated to an impaired f protein. | in this work we have characterized the virus (rsv(48)) present in passage 48 of a respiratory syncytial virus persistently infected murine macrophage-like cell culture. this virus was noncytopathic in macrophages and had a low-fusogenic activity in rsv-permissive cell lines, although the level of this activity varied among the different cell lines tested. the fusogenic activity of rsv(48) in vero cells, as evaluated by the number and size (nuclei per syncytium) of syncytia, was lower than that s ... | 2009 | 19014983 |